Repetitive Application Research Articles

Culture-based and molecular investigation of antibiotic and metal resistance in a semi-arid agricultural soil repeatedly amended with urban sewage sludge

Unsustainable agricultural intensification and climate change effects have caused chronic soil depletion in most arid and semi-arid croplands. As such, the land application of urban sewage sludge (USS) has been regulated in several countries as an alternative soil conditioner with recycling benefits. However, the risks of multi-contamination have made its agricultural reuse debatable. Accordingly, this study explored the long-term the impact of repetitive USS applications with increasing rates (0, 40, 80, and 120 t ha−1 year−1) on a sandy soil properties. A special focus was on the spread of antibiotic-resistant bacteria, metal-resistant bacteria and corresponding resistance genes in soil (ARB, MRB, ARGs and MRGs, respectively). The outcomes showed a dose-dependent variation of different soil parameters including the increase of heavy metal content and total heterotrophic bacteria (THB) up to the highest sludge application rate. Besides, the two last sludge lots applied in fall 2019 and 2020 contained cultivable ARB for all addressed antibiotics at much higher counts than in corresponding treated soils. Interestingly, the average index of antibiotic resistance (ARB/THB) increased in the USS used in fall 2020 compared to 2019 (from 6.2% to 9.4%). This indicates that factors such as fluctuations in wastewater quality, treatments operations, and extensive antibiotic use following the outbreak of the COVID-19 pandemic in early 2020 could have caused this variation. The molecular assessment of bacterial resistance resulted in the identification of three ARGs (mefA, sul1 and sul2), one MRG (czcA) and one integron (intI1). This might have implications on resistance co-selection, which can pose a threat to human health via contaminated crops.

Data scrambler knight tour algorithm

Nowadays, data scrambling remains a vital technique to protect sensitive information by shuffling it in a way that makes it difficult to decipher or reverse-engineer while still maintaining its usability for legitimate purposes. As manipulating the usability of the scrambled data remains a challenge on the background of risking losing data and getting them re-identified by attackers, scrambling and descrambling should be accomplished faster by not increasing data loss and re-identification risks. A scrambling algorithm must have a linear time complexity, still shuffling the data to minimize the risks further. A promising approach is based on the knight open tour problem, whose solutions appear like a random series of knight positions. Hence, a knight open tour algorithm is formalized, by which the knight seems to move chaotically across the chessboard. The formalization is presented as an indented pseudocode to implement it efficiently, whichever programming language is used. The output is a square matrix representing the knight open tour. Based on the knight tour matrix, data scrambler and descrambler algorithms are presented in the same manner. The algorithms have a linear time complexity. The knight-tour scrambling has a sufficiently low guess probability if an appropriate depth of scrambling is used, where the data is re-scrambled repetitively. The scrambling depth is determined by repetitive application of the chessboard matrix, whose size usually increases as the scrambling is deepened. Compared to the pseudorandom shuffling of the data along with storing the shuffled indices, the knight-tour descrambling key is stored and sent far simpler yet ensures proper data security.

Influence factors and improvement scheme on the breakdown behavior of pseudospark switch

Abstract High-power pulse generators are widely used in civil and military fields. The main switch directly determines the output characteristics of the high-power pulse generators, such as the voltage front time (tf). Pseudospark switches (PSS) show a promising future for middle voltage, high repetitive frequency pulse power applications. However, how to further improve the breakdown behavior without reducing its advantages is a challenging task. In this paper, the influence of operating parameters (anode voltage UA and gas pressure p) and structural parameter (number of cathode holes) on the breakdown behavior are investigated, the related mechanism are explained, and specific improvement schemes are proposed. It is found that the tf of the single channel PSS (SCPSS) decreased significantly with increasing p, but hardly varied with UA under moderate p. However, it is not a sound solution to increase the p excessively to reduce tf. Besides, increasing the number of cathode holes can obtain a shorter tf at low pressures (which implies superior repetition frequency performance). However, at 25 Pa, the jitter (which is defined as the standard deviation of tf in multiple tests) of the 2-channel PSS is larger than that of the SCPSS. And the jitter of the 4-channel and 8-channel PSS is also greater than 6 ns and 2 ns, respectively. Through experimental and simulation analyses, it can be explained as the stepwise penetration of the virtual anode and the non-simultaneous ignition of the channels. A scheme to increase the trigger energy (ε) has been adopted to improve the simultaneous ignition probability, while shortening tf and reducing jitter. After optimization, the good ignition probability of the 4-channel PSS has been improved to 82% and the jitter has been reduced to less than 1 ns at 25 Pa and 14.7 mJ.

Evaluation of pulsed field ablation lesion characteristics using an in vitro vegetable model.

In vitro models to evaluate cardiac pulsed field ablation (PFA) have not been well established. We sought to create a standardized vegetable model and staining protocol for assessing unipolar PFA using a surface electrode. We exposed potato slabs to unipolar PFA in a saline bath using a 3.5mm electrode catheter and grounding pad connected to a custom-built high-voltage generator. Lesions were clearly visualized after staining with 2,3,5-triphenyltetrazolium chloride (TTC) using a timed protocol to reveal a necrotic center and a periphery of electroporated cells with intact mitochondria. Lesion volume increased linearly with increasing voltage and logarithmically with repetitive PFA applications. The findings observed in this vegetable model using a TTC staining protocol are consistent with findings observed with cardiomyocytes.

Evaluation of the Effects of Repetitive Anaesthesia Administration on the Brain Tissues and Cognitive Functions of Rats with Experimental Alzheimer's Disease.

Introduction: We evaluated the effects of repeated ketamine, propofol, and ketamine + propofol administration on cognitive functions and brain tissue of elderly rat models with streptozotocin-induced Alzheimer's disease. Materials and Methods: Thirty elderly male Wistar Albino rats were divided into five groups: control (Group C), Alzheimer's (Group A), Alzheimer's + ketamine (Group AK), Alzheimer's + propofol (Group AP), and Alzheimer's + propofol + ketamine (Group APK). Alzheimer's disease was induced in Groups A, AK, AP, and APK via intracerebroventricular streptozotocin. Four weeks after surgery, ketamine, propofol, and ketamine + propofol were administered intraperitoneally for 3 days to Groups AK, AP, and APK, respectively. The radial arm maze test (RAMT) was performed in the initial, 1st, 2nd, 3rd, and 4th weeks after surgery and daily following anaesthesia. Blood and brain tissue samples were obtained. Results: The RAMT results of Groups A, AK, AP, and APK decreased compared to Group C 2 weeks after Alzheimer's disease onset. Compared to Group A, the RAMT results increased in Groups AK and APK after the first anaesthesia, and in Group AP after the second anaesthesia. Brain tissue paraoxonase-1 (PON-1) and catalase (CAT) activities were low, and the thiobarbituric acid reactive substance (TBARS) level was high in Group A compared to Group C. TBARS levels of Groups AP and APK were lower than Group A, while CAT activity was higher. PON-1 activity was higher in Groups AK, AP, and APK than in Group A. Histopathological changes decreased in Groups AP and AK. A decrease in p53 was found in Group C compared to Group A. Ketamine and propofol were found to be effective at Bcl-2 immunoexpression, but a decrease in Caspase-3 was observed in Group APK. GFAP immunoexpression increased in Group A compared to Group C and in Group AP compared to Group AK. Conclusions: Repetitive anaesthesia application was found to positively affect cognitive functions. This was supported by histopathological and biochemical markers.

Optimizing of heat transfer and flow characteristics within a roughened solar air heater duct with compound turbulators

AbstractThermal systems for solar air heating have been widely used in both industrial and residential contexts, and are essential for converting and recovering solar energy. Thermal performance in solar air heaters (SAHs) can be improved through the repetitive application of artificial roughness to the surfaces. This research work includes a numerical evaluation of SAH performance with artificial rough surfaces made up of combined transverse trapezoidal ribs and chamfered grooves. The ANSYS Fluent software version 2023 R1 was used to simulate SAH with varying relative roughness pitch (), relative roughness heights (), and Reynolds number (). The RNG model was chosen to forecast an enhancement in Nusselt number (), friction factor (), and thermohydraulic performance factor (TPF) for the proposed roughness. Out of multiple roughness parameters analyzed, it was determined that the compound turbulator with and , were the most effective. The TPF for this scenario was determined to be 2.12 at . Finally, a numerical based empirical correlations for and in terms of Re, , and were developed.

Periscapular Strength Profile Changes in Collegiate Baseball Pitchers Over the Course of a Season.

Repetitive application of high forces to the shoulder and scapular musculature during the pitching motion over the course of a collegiate baseball season may lead to changes in strength and increased fatigue, potentially predisposing pitching athletes to injury. The purpose of this study was to investigate periscapular strength profiles of Division I collegiate baseball players over the course of a season. This study was a retrospective data analysis of 18 Division I baseball pitchers. Isometric scapular plane abduction (scaption), external rotator, internal rotator, middle trapezius, and lower trapezius isometric strength was measured on throwing arm of each subject using a MicroFET 2 handheld dynamometer. Data were collected in a single session at the preseason, midseason, and postseason of the college baseball season, which spanned a five-month period. A repeated measures ANOVA was utilized to determine if there was a significant change in periscapular strength across the baseball season. Over the three time-points, all strength values decreased for each muscle group, with decreases ranging from 3-14%. Changes in scaption strength values were statistically significant (p=0.018, partial eta squared =0.284) with an 8% reduction over the entire season, specifically with a 5% decline from mid-season to post-season. While external rotator, internal rotator, middle trapezius, and lower trapezius strength values all decreased over the course of the season (range 0.02kg to 1.8kg), these differences were not statistically significant. However, the middle trapezius strength value change (1.3kg loss) exceeded the minimal detectible change. Periscapular muscles in baseball pitchers diminish in strength over the course of a collegiate baseball season. Understanding strength changes over the course of a season may influence training and therapeutic interventions. 3b.

Preclinical Study of Pulsed Field Ablation of Difficult Ventricular Targets: Intracavitary Mobile Structures, Interventricular Septum, and Left Ventricular Free Wall.

Endocardial catheter-based pulsed field ablation (PFA) of the ventricular myocardium is promising. However, little is known about PFA's ability to target intracavitary structures, epicardium, and ways to achieve transmural lesions across thick ventricular tissue. A lattice-tip catheter was used to deliver biphasic monopolar PFA to swine ventricles under general anesthesia, with electroanatomical mapping, fluoroscopy and intracardiac echocardiography guidance. We conducted experiments to assess the feasibility and safety of repetitive monopolar PFA applications to ablate (1) intracavitary papillary muscles and moderator bands, (2) epicardial targets, and (3) bipolar PFA for midmyocardial targets in the interventricular septum and left ventricular free wall. (1) Papillary muscles (n=13) were successfully ablated and then evaluated at 2, 7, and 21 days. Nine lesions with stable contact measured 18.3±2.4 mm long, 15.3±1.5 mm wide, and 5.8±1.0 mm deep at 2 days. Chronic lesions demonstrated preserved chordae without mitral regurgitation. Two targeted moderator bands were transmurally ablated without structural disruption. (2) Transatrial saline/carbon dioxide assisted epicardial access was obtained successfully and epicardial monopolar lesions had a mean length, width, and depth of 30.4±4.2, 23.5±4.1, and 9.1±1.9 mm, respectively. (3) Bipolar PFA lesions were delivered across the septum (n=11) and the left ventricular free wall (n=7). Twelve completed bipolar lesions had a mean length, width, and depth of 29.6±5.5, 21.0±7.3, and 14.3±4.7 mm, respectively. Chronically, these lesions demonstrated uniform fibrotic changes without tissue disruption. Bipolar lesions were significantly deeper than the monopolar epicardial lesions. This in vivo evaluation demonstrates that PFA can successfully ablate intracavitary structures and create deep epicardial lesions and transmural left ventricular lesions.

Repetitive dynamic matrix control for systems with periodic specifications

This paper proposes a Repetitive Dynamic Matrix Control (RDMC) for systems with periodic specifications. The new algorithm is able to track periodic references and reject repetitive disturbances with a known period based on a modified prediction error. A repetitive version of the Generalized DMC (GDMC) is also proposed such that it can be applied to control open-loop unstable systems. Only the step-response coefficients are required to describe the dynamical system such that the RDMC preserves the modeling simplicity of the Dynamic Matrix Control (DMC). The proposed solution can be interpreted as an extension of the DMC for repetitive control applications. A data-driven filter design is proposed in order to ensure null prediction steady-state error in the presence of periodic disturbances even for unstable open-loop systems. Two case studies are presented to show the usefulness of the proposed strategy for control systems with periodic specification and to illustrate the typical advantages and drawbacks of the proposed repetitive control extension of the DMC algorithm.

Correction: Takahashi et al. Estimating the Dominant Life Phase Concerning the Effects of Battery Degradation on CO2 Emissions by Repetitive Cycle Applications: Case Study of an Industrial Battery System Installed in an Electric Bus. Energies 2023, 16, 1508

In the original publication [...]

Composing MPC With LQR and Neural Network for Amortized Efficiency and Stable Control

Model predictive control (MPC) is a powerful control method that handles dynamical systems with constraints. However, solving MPC iteratively in real time, i.e., implicit MPC, remains a computational challenge. To address this, common solutions include explicit MPC and function approximation. Both methods, whenever applicable, may improve the computational efficiency of the implicit MPC by several orders of magnitude. Nevertheless, explicit MPC often requires expensive pre-computation and does not easily apply to higher-dimensional problems. Meanwhile, function approximation, although scales better with dimension, still requires pre-training on a large dataset and generally cannot guarantee to find an accurate surrogate policy, the failure of which often leads to closed-loop instability. To address these issues, we propose a triple-mode hybrid control scheme, named Memory-Augmented MPC, by combining a linear quadratic regulator, a neural network, and an MPC. From its standard form, we derive two variants of such hybrid control scheme: one customized for chaotic systems and the other for slow systems. The proposed scheme does not require pre-computation and is capable of improving the <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">amortized</i> running time of the composed MPC with a well-trained neural network. In addition, the scheme maintains closed-loop stability with <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">any</i> neural networks of proper input and output dimensions, alleviating the need for certifying optimality of the neural network in safety-critical applications. <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Note to Practitioners</i> —This article was motivated by the need to reduce the amortized cost of MPC in repetitive industrial robotic applications, where long-term operational cost is important and safety is critical. Examples of such applications include factory robotic arm manipulation and fixed-route quadcopter payload transport. Unlike explicit MPC or function approximation, our approach does not require any pre-computation or pre-training. Rather, it attains task proficiency over time by learning a surrogate neural network on the spot and by gradually replacing the costly MPC with the more efficient surrogate model so long as safety permits. Consequently, the proposed scheme incurs a learning cost during the initial phase of the deployment but usually becomes more adept on the task afterwards, leading to amortized efficiency.

Mathematical Modeling of PI3K/Akt Pathway in Microglia.

The motility of microglia involves intracellular signaling pathways that are predominantly controlled by changes in cytosolic Ca2+ and activation of PI3K/Akt (phosphoinositide-3-kinase/protein kinase B). In this letter, we develop a novel biophysical model for cytosolic Ca2+ activation of the PI3K/Akt pathway in microglia where Ca2+ influx is mediated by both P2Y purinergic receptors (P2YR) and P2X purinergic receptors (P2XR). The model parameters are estimated by employing optimization techniques to fit the model to phosphorylated Akt (pAkt) experimental modeling/in vitro data. The integrated model supports the hypothesis that Ca2+ influx via P2YR and P2XR can explain the experimentally reported biphasic transient responses in measuring pAkt levels. Our predictions reveal new quantitative insights into P2Rs on how they regulate Ca2+ and Akt in terms of physiological interactions and transient responses. It is shown that the upregulation of P2X receptors through a repetitive application of agonist results in a continual increase in the baseline [Ca2+], which causes the biphasic response to become a monophasic response which prolongs elevated levels of pAkt.

Mechanical stimulation and electrophysiological monitoring at subcellular resolution reveals differential mechanosensation of neurons within networks

A growing consensus that the brain is a mechanosensitive organ is driving the need for tools that mechanically stimulate and simultaneously record the electrophysiological response of neurons within neuronal networks. Here we introduce a synchronized combination of atomic force microscopy, high-density microelectrode array and fluorescence microscopy to monitor neuronal networks and to mechanically characterize and stimulate individual neurons at piconewton force sensitivity and nanometre precision while monitoring their electrophysiological activity at subcellular spatial and millisecond temporal resolution. No correlation is found between mechanical stiffness and electrophysiological activity of neuronal compartments. Furthermore, spontaneously active neurons show exceptional functional resilience to static mechanical compression of their soma. However, application of fast transient (∼500 ms) mechanical stimuli to the neuronal soma can evoke action potentials, which depend on the anchoring of neuronal membrane and actin cytoskeleton. Neurons show higher responsivity, including bursts of action potentials, to slower transient mechanical stimuli (∼60 s). Moreover, transient and repetitive application of the same compression modulates the neuronal firing rate. Seemingly, neuronal networks can differentiate and respond to specific characteristics of mechanical stimulation. Ultimately, the developed multiparametric tool opens the door to explore manifold nanomechanobiological responses of neuronal systems and new ways of mechanical control.

Evaluation of the effect of intravitreal injections on corneal epithelial, scleral and limbal region changes in diabetic retinopathy by AS-OCT.

The aim of the study is to evaluate the effects of diabetic retinopathy and intravitreal injections on the corneal, limbal and scleral areas. Patients with diabetes mellitus at different diagnosis and treatment levels were compared among themselves and with the control group in terms of corneal, limbal and scleral aspects with the help of anterior segment optical coherence tomography. In addition, clinical tests such as tear break-up time, Schirmer test-I and ocular surface disease index questionnaire were applied to the patients and the difference between the groups was investigated. When the groups were examined in terms of BUT, SCH-I and OSDI, there was a statistically significant difference between control group and diabetic group(p < 0.05). In the limbal region, all measurements are higher than in patients with diabetic eye involvement. Thinning was detected in the scleral area with intravitreal injection (p < 0.05). It should be known that DM may cause undesirable changes in the limbal region, and the importance of non-invasive detection with AS-OCT should not be forgotten. Since intravitreal injections for DME cause thinning of the sclera, it can cause various complications, and it may be recommended to change the quadrant in repetitive injection applications.

Life Parameters and Physiological Reactions of Cotton Aphids Aphis gossypii (Hemiptera: Aphididae) to Sublethal Concentrations of Afidopyropen

The massive and repetitive application of synthetic insecticides for the management of cotton pests results in the accumulation of resistance in Aphis gossypii Glover, a destructive pest worldwide. New chemistries are needed for pest management. Afidopyropen exhibits high efficacy against piercing-sucking pests and has been applied as a complementary alternative insecticide against aphids. This study was conducted to investigate the lethal and sublethal effects of afidopyropen on the life parameters and physiological responses of A. gossypii. Detoxifying enzyme activities and expression levels of P450 genes were compared after exposure to three generations of afidopyropen. Bioassay results indicate that afidopyropen possessed the highest toxicity, with a LC50 value of 0.30 mg/L. Sublethal concentrations (LC5 and LC10) caused adverse impacts on the F0 generation, reducing adult longevity and fecundity. A high concentration (LC10) also caused adverse effects on the F1 generation, while a low concentration (LC5) stimulated the fecundity. After continuous treatments with afidopyropen, the susceptibility decreased. GSTs and P450 were induced through sublethal concentrations; moreover, their activities in the F3 generation were higher than that in the F0 generation. Furthermore, the expression levels of 12 P450 genes in the F3 generation were higher than those in F0 generation. In conclusion, afidopyropen has excellent acute toxicity and continuous control effects on A. gossypii. GSTs and P450 may play important roles in the resistance of A. gossypii to afidopyropen.

Potential use of nanofibrillated cellulose-loaded cationic starch solutions as coating formulation for recycled fluting papers

Effects of cellulose nanofibers (CNF) and cationic starch (CS) were evaluated as coating components relative to the physical and mechanical properties of fluting papersheets fabricated from recycled corrugated cardboard fibers. Fabricated fluting papers were subjected to size press applications by three different coating blends. Coating suspensions were prepared at various concentrations of CNF (0.5%, 1%, 2%, 3%, and 4%) and 4 wt% CS, and the same amounts of CS/CNF. The paper sheets were fabricated using size press machine as three-time repetitive applications, followed by one-time drying section, and compared to uncoated, CS-coated, and CNF-coated papers. The application of CNF suspensions increased tensile indices up to 11.7%. Moreover, CS/CNF suspensions resulted in a 67.2% increase in tensile index values. The coating of CS/CNF suspensions increased the burst index values by 163% at the CS+1%CNF concentration when compared to the control pulp. Surface application of prepared suspensions reduced the porosity of the samples under all conditions. The highest reduction in the air permeability was observed in the CS+4%CNF-coated samples as 91.5%. It can be concluded that the superficial applications of CNF on the physical and mechanical properties of recycled fluting paper was more effective in the presence of CS.

Weed control with saturated steam in organic highbush blueberry

Weed management is often a predominant and costly problem in the production of organic blueberries. Geotextile weed fabrics of woven polyethylene are widely used in organic blueberry fields to suppress weeds growing within the rows. Weeds, such as Convolvulus arvensis L., grow at the base of the blueberry plants or through openings and around the edges of the weed fabric, thus requiring hand weeding. This study evaluates the integration of saturated steam (SS), a rotary brush (RB), and organic herbicides for weed control in blueberries. Dose–response studies indicated that SS applied at 121°C and at 7.4 m3 ha−1 of steam (3,655 MJ ha−1) resulted in over 90% control and a reduction in the dry weights of C. arvensis. When treatments were directed to the base of the blueberry plants, SS at 7.4 m3 ha−1 provided 80% control of C. arvensis 28 days after treatment (DAT) and was comparable to hand weeding. Both of these treatments outperformed capric plus caprylic acid (CC) (33.2 kg ai ha−1) or ammonium nonanoate (AN) (24.3 kg ai ha−1) applications, despite C. arversis regrowth being observed. Four repetitive basal applications of SS of up to 29.6 m3 ha−1 over two consecutive years caused minimal and transient damage to new basal shoots of ‘Elliot’ and ‘Duke’ blueberries; basal shoot cross-sectional area compared with the non-treated was unaffected. In contrast, basal application of AN treatments damaged or killed basal shoots. When treatments were applied to the edge of the weed fabric, SS (7.4 m3 ha−1) reduced weed biomass by 42% to 93% at 28 DAT compared with the non-treated. The RB treatment reduced weed biomass from 72% to 99% in all experiments, while CC and AN reduced biomass by 18% to 54%. A partial budget analysis indicated that SS and the RB were 3- and 6.5-fold less expensive than organic herbicides, respectively. Integrating physical (SS) and mechanical (RB) treatments improved weed control. The latter, however, damaged the weed-suppressing fabric where preexisting holes were present, generated dust, and increased the chance of fruit contamination. The SS was safe for the weed-suppressing fabric and the blueberry, but weed regrowth following treatment and copious water requirements hindered its feasibility.

Investigating change of discomfort during repetitive force exertion though an exoskeleton cuff

This article investigates discomfort development for forces exerted repetitively and for extended durations through a rigid cuff.Three force patterns, chosen to mimic exoskeleton use, were applied to the thigh of 15 healthy participants for 30 min. Changes in perceived comfort and skin effects were recorded.Discomfort was detected at normal forces ranging from 40 to >230 N. Repetitive force application triggered discomfort after a median of 4.1 min (normal force only) and 5.4 min (normal and shear force) respectively. Discomfort increased over time but the repetitive force applications did generally not result in pain and there were no significant differences between repetitive loading patterns.Exoskeleton design and use should be informed by comfort thresholds specific to prolonged repetitive loading. Large interindividual differences in perception of discomfort limit the possibilities for generally applicable comfort thresholds. Further research is needed to investigate how patient groups perceive such repetitive loading.

Co-exposure of dimethomorph and imidacloprid: effects on soil bacterial communities in vineyard soil.

In Taiwan, the pesticides dimethomorph and imidacloprid are recommended for pest control in vineyards. Therefore, tank-mixing of these two pesticides is usually a routine practice before application. This study analyzed the influence of vineyard soil microbial flora under the recommended and high dosages (100 times the recommended dosage) of dimethomorph and imidacloprid. Individual and combined applications of pesticides were also tested through batches of soil incubation experiments. Four treatments-control (C), dimethomorph (DT), imidacloprid (IM), and mixed application of dimethomorph and imidacloprid (ID)-were used in the experimental design. From the soil metabolism, no significant reaction was observed after 2 months in the recommended dosage group, regardless of whether the pesticides were being applied individually or combined. For the high dosage, imidacloprid showed a higher effect than the co-exposure treatments, showing a possible prolonged effect after its repetitive application. From PCoA analysis, pesticide treatments altered the soil ecology after 2 months, and the effect of imidacloprid can be explicitly observed at high dosages. At the phylum level, Acidobacteria can indicate pesticide application around the recommended dosage. It was inhibited by ID on day 7 and was augmented by all pesticides on day 63. The effect of the recommended dosage of pesticide mixtures after 2 months of incubation was revealed in the minor families Gemmataceae and Pirellulaceae, while the high dosage treatments affected both the core and the minor families. Our findings verified the changes in the composition of microbial communities upon pesticide application, which would affect carbon, nitrogen, sulfur, phosphorous cycles, and contaminant removal ability within the vineyard.

Analysis and Design of a Novel LLC CCPS for Repetitive Pulsed Power Applications

This paper presents a novel type of capacitor charging power supply (CCPS) based on LLC resonant DC/DC converter for repetitive pulsed power applications, such as pulsed lasers. Because the load of CCPS is almost capacitive, leading to a quickly varying of output voltage during the charging period, conventional methods are not appropriate and not accurate enough for analyzing the transient characteristics of this proposed LLC CCPS. Thus, an equivalent resistance method based on first harmonics analysis (FHA) method is proposed and parasitic capacitor effect is analyzed too. In order to improve the stability and robustness of the current control system of the proposed LLC CCPS, a PI+LPF type controller combining LUT-based feed-forward is applied. Based on the theories above, a prototype with 200V input and maximum 1kV/2kW output is demonstrated. A peak efficiency of 97.85% and 2A peak average output charging current is achieved and experiments of repetitive pulsed power with 1Hz are carried out. The results prove the superiority of the proposed LLC CCPS. This text explores new topology options for CCPS and aims to improve their performance, the proposed LLC CCPS shows great engineering application value and potential on repetitive pulsed power applications.