Regions Of Croatia Research Articles

The Labin Region, an ecologically vulnerable geographical area in Croatia: Mortality characteristics in an area polluted by industrial over a 40-year period.

The history of the Labin region in Croatia includes intensive industrial development with repercussions of pollution on environment and health. Assuming that prolonged exposure to polluted environments causes qualitative changes in mortality, the aim was to analyse the mortality characteristics of the population of the Labin Region for the 1968-2008 period based on data from the Croatian Bureau of Statistics. Public health and social opportunities in this geographical area carry a long-term burden of exposure to an industrial polluted environment with outcomes expressed by mortality or/and morbidity in the population. This study includes data on 11,903 deaths, most of which due to diseases of the circulatory, respiratory and digestive systems as well as neoplasms. In the third and fourth decade of the study period, a group of neoplasms showed significant increases, while the increase in respiratory diseases were more gradual. The female population died mostly from diseases of the circulatory and endocrine system as well as neoplasms, while the male population mainly died from diseases of the digestive system and external causes. This research provides guidelines that could create better public health, raising the quality of life and contribute to a future environmental protection in local communities by targeted policies.

Physical Activity and Sedentary Behaviour in Croatian Preschool Children: A Population-Based Study

This study aimed to determine the differences in physical activity and sedentary behaviour in preschool children living in different geographical regions. Preschool children (N=1625) and their parents from different parts of Croatia, from urban and rural settlements, participated in this cross-sectional, population-based study. Parents completed the Netherlands Physical Activity Questionnaire (NPAQ), a measure of physical activity and sedentary behaviour in children. The main results of this study show differences in physical activity and sedentary behaviour in preschool children living in four geographical regions in Croatia (F=4.45; p<0.01). The least physically active are children from a continental area that gravitates to the capital city, while the most active are children from a southern coastal region. Sedentary behaviour is the greatest in the rural eastern Croatian continental region. Higher physical activity and lower sedentary activities in young children living in coastal compared to continen- tal regions show possible specific advantages of Mediterranean climate in general. The practical importance of information obtained in this study is a need for a specific intervention strategy for improving physical activity in continental preschool institutions.

Identification of powdery mildew resistance in wild grapevine (Vitis vinifera subsp. sylvestris Gmel Hegi) from Croatia and Bosnia and Herzegovina

Wild grapevine (Vitis vinifera subsp. sylvestris) is widely recognized as an important source of resistance or tolerance genes for diseases and environmental stresses. Recent studies revealed partial resistance to powdery mildew (Erysiphe necator, PM) in V. sylvestris from Central Asia. Here, we report resistance to PM of V. sylvestris collected from different regions of Croatia and in seedling populations established from in situ V. sylvestris accessions. Ninety-one in situ individuals and 67 V. sylvestris seedlings were evaluated for PM resistance according to OIV 455 descriptor. Three SSR markers (SC47-18, SC8-071-0014, and UDV-124) linked to PM resistance locus Ren1 were used to decipher allelic structure. Nine seedlings showed resistance in in vivo evaluations while leaf disk assays revealed three PM-resistant accessions. One V. vinifera cultivar used as a control for PM evaluations also showed high phenotypic resistance. Based on the presence of one or two resistance alleles that are linked to the Ren1 locus, 32 resistant seedlings and 41 resistant in situ genotypes were identified in the investigated set. Eight seedlings showed consistent phenotypic PM resistance, of which seven carried one or two alleles at the tested markers. This study provides the first evidence of PM resistance present within the eastern Adriatic V. sylvestris germplasm.

The Content of Cobalt, Silver and Vanadium in Raw Cow’s Milk in Croatia and Risk Assessment for Consumers

Concentrations of selected trace elements Ag, Co and V in raw milk sampled from four geographical regions in Croatia were measured. Silver, Co and V were detected above the limit of detection within the range of 9.52%-30.8%, 1.6%-12.1% and 12.4%-30.8%. Silver concentrations were not detected in milk samples from the Croatian Littoral and Mountainous Croatia (CL-MC) region. Similar Ag content was found in Southern, Eastern and Central Croatia. The lowest mean of Co and V of 33.2 and 83.8µg kg-1 were found in the CL-MC region while the highest of 49.8 and 136.9µg kg-1 was found in Central Croatia. There were no statistically significant differences in Ag, Co and V contents between the four regions. The estimated daily dietary intakes (EDI) of total mean and total 95th percentile values of Ag, Co and V showed lower values in comparison with available EFSA health-based limits.

Adherence to the Mediterranean Diet among Children and Youth in the Mediterranean Region in Croatia: A Comparative Study.

The Mediterranean diet (MD) is considered one of the healthiest dietary patterns. The aim of this study was to assess MD adherence in children and youth living in the Mediterranean region in Croatia and evaluate the differences in adherence to the MD among different educational stages. In total, 2722 individuals aged 2 to 24 years were enrolled in this study. Subjects were divided into different groups according to the Croatian educational system. Mediterranean Diet Quality Index (KIDMED) was used to assess adherence to the MD. In the total sample, the adherence to the MD was poor in 19.2%, average in 60.8%, and good in 20.1% of the study participants. The prevalence rate of poor adherence to the MD increased with higher educational stage, i.e., the highest prevalence rate of poor MD adherence was observed for college students (39.3%). Children having a higher number of snacks on days-off, those with lower physical activity, and not having breakfast together with a family are more likely to have poor MD adherence, while children having a higher number of snacks on working days are less likely to have a poor MD. The results of this study showed low adherence to the principles of the MD, confirming the need for improvement of adherence to the MD pattern in the studied population.

Application of spatial- and diachronic-process analysis of landscape development in Central Lika, Croatia from 1980 to 2012

The paper presents structural features and the process and intensity of landscape changes in the Central Lika Region of Croatia, in the transitional, post-socialist and post-war periods (1980–2012). The aim of this paper is to analyse regularities and interrelations between the changes in structural features and the intensity of the processes that cause change. By using GIS spatial analysis methods, the shape, position, and condition of landscape patches were defined for each year. The diachronic process analysis, with the application of a specially-developed index — the Landscape Development Index (di) — made it possible to determine the intensity of development of individual landscape elements. The landscape was observed using the concept of Land Use/Land Cover (LULC) and, for this purpose, data from the CORINE Land Cover database were used for 1980 and 2012. The results showed fragmentation and an increase in the number and complexity of patches, as well as a decrease of the core patch area. Consequently, the increase of the share of edge areas has increased vulnerability to external influences and changes. Applying the Landscape Development Index (di) showed advantages compared to a standard comparison of the two conditions or synthetic indices, enabling the intensity of each observed phenomenon/process to be determined, and allowing for deeper spatial and temporal analysis as well as better understanding of landscape development.

In the Quest of Sustainability Principle "Reuse": Awareness of Northern Croatia Adult Population

The world has found itself spinning ever faster and economies producing ever more. But this process cannot go on forever. The Earth we live on is one and only and it has limited resources in comparison with what the people are ready to use. Sustainability of the environment is bitten by recklessness of many but critical voices are being raised in recent decades. As Stahel (2016) noted, contrary to nature and its processes, people are still primarily functioning in linear way – "make, use, dispose". This is fundamentally supported by consumerism approach and the view that the economy prospers only by permanent growth driven by infinite desires of consumers (Rojek 2004). But critical voices emphasize the social role of entrepreneurship (Zahra and Wright 2016) with the conclusion that the entrepreneurship can benefit from lowering their environmental impact (wasteful resource usage) thus also resulting in personal and societal benefits, beside the economic value creation (see about the triple bottom line also in Klarin 2018). This also spurs emerging of new business opportunities (Korhonen, Honkasalo, and Seppälä 2018). As Zahra and Wright (2016) develop it further, the businesses have to "move from 'do no harm' to 'do good'". Sustainability can be promoted by the circular economy (CE). CE is a concept in which instead of linear flow, outputs from one part of the economic process are kept in this part longer (reused, repaired, refurbished) and eventually recycled as the input for the next part of the process. This reduces the waste for the landfill disposal and the needs for resources. (Geisendorf and Pietrulla 2018; Korhonen et al. 2018) Although the definitions clearly rely on the actions of the producers (e.g. companies), we cannot forget the market pull factor and the role of consumers. Raising awareness of the consumers about the consequences of their actions can be an important factor for hurrying up introduction of the CE principles, and especially increased recycling in their everyday operating practices (Abe et al. 2014). Since CE and its principles represent a complex phenomenon, in this paper the focus of research is on the "Reuse" principle and the awareness and willingness of the adult population in northern Croatia region to engage in the reusing the consumer goods. This region is economically strong with higher contribution of the manufacturing industry to the GDP compared to the rest of the country which makes it a good candidate for this preliminary study. Keywords: Sustainability, Circular Economy, Reuse, Reduce, Recycle

Essential and potentially toxic elements in raw milk from different geographical regions of Croatia and their health risk assessment in the adult population

Concentrations of selected elements were measured in raw cow milk collected from four geographical regions in Croatia. The highest mean concentrations of Al, Ba, Fe and Zn were measured in milk from Central Croatia. Milk from the Croatian Littoral and Mountain region showed the highest mean content of Cd, Cr, Cu, Ni and Pb. The highest mean concentrations of As and Se were found in milk from Southern Croatia, while Mn had the highest values in Eastern Croatia. Mercury was not determined above the detection limit in any samples. Significant differences in the concentrations of Al, Ba, Se and Zn were found among the regions. A decrease in the Pb content was recorded compared to previous research in milk in Croatia. Estimation of the potential harmful effects of toxic and trace elements through milk consumption by comparing the established highest mean with toxicological limits showed low exposure with regard to Al, As, Ba, Cr, Cu, Hg, Fe, Ni, Se, Pb and Zn in adult populations in Croatia. The only exception was Cd concentrations in milk, which gave a higher contribution to toxicological limits, especially for consumers consuming greater quantities of milk per week.

The Efficacy of Seed Treatments on Major Sugar Beet Pests: Possible Consequences of the Recent Neonicotinoid Ban

Sugar beet production remains unprotected after the ban on neonicotinoids, while pest pressure is increasing. Although the organic approach to agriculture is highly welcomed, the question remains whether it will be possible to grow sugar beet without pesticides. The aim of this study is to determine the efficacy of seed treatments with neonicotinoids on the main sugar beet pests, to determine the susceptibility of the pests under the specific climatic conditions and to discuss possible consequences of the ban of neonicotinoids on the future of sugar beet production in southeast Europe. The study was conducted in two different climatic regions in Croatia in two consecutive years. The tested variants were: seed coated with imidacloprid, seed coated with a combination of thiamethoxam and teflutrin and untreated control. Our results showed that seed coatings with imidacloprid and thiamethoxam provided satisfactory protection against wireworms, flea beetles and sugar beet weevils at low population pressure. These pests are regular pests of sugar beet in southern and eastern Europe and therefore need to be controlled. Caterpillars and aphids were present in low populations, so the efficacy of the insecticides tested cannot be determined with certainty. A further research program is needed to find alternative solutions and develop easily implementable strategies for all sugar beet pests. We would propose an authorization of neonicotinoids for seed treatment of sugar beet in the regions with high infestation pressure of the main sugar beet pests.

Mapping the Human Leukocyte Antigen Diversity among Croatian Regions: Implication in Transplantation.

In the present study, HLA allele and haplotype frequencies were studied using the HLA data of 9277 Croatian unrelated individuals, typed using high-resolution methods for the HLA-A, -B, -C, and -DRB1 loci. The total numbers of observed alleles were 47 for HLA-A, 88 for HLA-B, 34 for HLA-C, and 53 for HLA-DRB1. HLA-A∗02:01 (29.5%), B∗51:01 (10.5%), C∗04:01 (15.8%), and DRB1∗16:01 (10.4%) were the most frequent alleles in the Croatian general population. The three most frequent haplotypes were HLA-A∗01:01~C∗07:01~B∗08:01~DRB1∗03:01 (4.7%), HLA-A∗03:01~C∗07:02~B∗07:02~DRB1∗15:01 (1.7%), and HLA-A∗02:01~C∗07:01~B∗18:01~DRB1∗11:04 (1.5%). Allele and haplotype frequencies were compared between national and regional data, and differences were observed, particularly in the North Croatia region. The data has potential use in refining donor recruitment strategies for national registries of volunteer hematopoietic stem cell donors, solid organ allocation schemes, and the design of future disease and anthropological studies.

Klebsiella pneumoniae carbapenemase (KPC) in urinary infection isolates.

Recently, emergence of carbapenem-resistance, in particular due to Klebsiella pneumoniae carbapenemase (KPC), was observed among K. pneumoniae causing urinary tract infections in Croatia. The aim of the study was to characterize, antimicrobial susceptibility, carbapenem resistance, virulence traits and plasmid types of the urinary KPC positive isolates of K. pneumoniae. The antimicrobial susceptibility to a wide range of antibiotics was determined by broth microdilution method. The transferability of meropenem resistance was determined by conjugation (broth mating method) employing Escherichia coli J63 strain resistant to sodium azide. Genes encoding broad and extended-spectrum β-lactamases, plasmid-mediated AmpC β-lactamases, group A and B carbapenemases, and carbapenem hydrolyzing oxacillinases (blaOXA-48like), respectively, were determined by Polymerase chain reaction (PCR). In total 30 KPC-positive K. pneumoniae urinary isolates collected from different regions of Croatia were analysed. The isolates were uniformly resistant to all tested antibiotics except for variable susceptibility to gentamicin, sulphamethoxazole/trimethoprim, and colistin, respectively. Four isolates were resistant to colistin with MICs values ranging from 4 to 16mg/L. All tested isolates were susceptible to ceftazidime/avibactam. Sixteen isolates transferred meropenem resistance to E. coli recipient strain by conjugation. Other resistance markers were not co-transferred. PCR was positive for blaKPC and blaSHV genes in all isolates whereas 13 isolates tested positive also for blaTEM genes. PCR based replicon typing (PBRT) revealed the presence of FIIs in 13 and FIA plasmid in two strains. The study showed dissemination of KPC-producing K. pneumoniae in urinary isolates, posing a new epidemiological and treatment challenge. Sulphamethoxazole/trimethoprim, colistin, and ceftazidime/avibactam remain so far, as the therapeutic options.

Overall Seismic Risk in Bosnia-Herzegovina/Dalmatia Region of Croatia and Elements of Perfect Crisis in an Earthquake Aftermath

Bosnia-Herzegovina and southern-coastal/hinterland-region of Dalmatia, Republic of Croatia are a part of the same seismo-tectonic province and share a considerable and real threat of high-magnitude earthquakes. Numerous Magnitude 6 (Richter) and above earthquakes have been recorded in the past 500 years and some of them have resulted in a considerable loss of life, material and even prestige or geopolitical significance (e.g. the demise of Ragusa in the earthquake of 1667). Given the propensity of the region for destructive earthquakes, complex geomorphological framework and challenging infrastructure, still recovering in parts from the Yugoslav civil wars of the 1990s., the region may yield a “perfect” crisis in the aftermath of a major earthquake event. Taking into consideration unchecked development of several metropolitan areas, lack of oversight and permitting, decaying infrastructure as well as unresolved political ambiguities and territorial disputes, a potential destructive earthquake may create several cascading crises, especially if it coincides with some other challenging events (e.g. winter storms). This study is taking into consideration several scenarios, their possible effects and resulting conditions upon which cascading crises may arise in the aftermath of a magnitude 7+ earthquake affecting several major urban areas in southern and central Bosnia-Herzegovina and the southern Dalmatian region of Croatia. These scenarios are intended to provide training aids and risk assessments in countering the destructive forces after the earthquake, expected to test the current crisis-management models.

Behaviour of Uniform Drava River Sand in Drained Condition—A Critical State Approach

Numerous triaxial tests on sand and sand-like materials have been performed worldwide during the past several decades. Their results provided a development of the advanced soil constitutive models and laboratory testing devices, as well as the establishment of a worldwide database of different types of uniform sandy materials. From such research, the critical state and steady state theory has emerged as one of the most useful tool for the modelling of a soil behaviour. This paper presents the results of static drained tests performed on the uniform Drava River sand from the Osijek region in Croatia. The main aim was to determine the shear behaviour and critical state, given that these characteristics are mostly unknown for the tested sand material. A series of detail triaxial tests were performed in drained conditions for three different initial relative densities, DR, and two different loading directions; e.g., axial compression and axial extension. In total, 18 drained tests were performed. The study indicated that the value of 33.75∘ is the critical friction angle for the tested sand. The relative density of 57% is determined as the critical relative density. Additionally, the study confirmed the difference in critical state for compression and extension loading. In addition, the results indicate that the sample preparation procedure has an important impact on the critical state of loosely prepared sandy samples. These results give the first insights into the behaviour of the Drava River sand, which can generally contribute to the worldwide sand behaviour knowledge base.

Wolf diet and prey selection in Croatia

Tensions between humans and wolves have led to intensive worldwide studies of wolf feeding habits and their relative preference for domestic and wild ungulates. The aim of this study was to provide further insight into the diet composition of wolves in Croatia, based on stomach contents of dead wolves. We examined spatial variation in wolf diet and prey selection relative to availability of wild and domestic animals. Furthermore, we aimed to determine selectivity in feeding habits in relation to wolf gender and age. The study was conducted on the stomach contents of 42 gray wolves (18 females, 24 males). Samples were collected from three regions of Croatia with different ratios of domestic and wild prey availability. The density ratio of domestic to wild ungulates increased gradually from north-west (5.8), through central (11.6) to south-east (134) Croatia. Wolf diet followed this pattern with the ratio of domestic animals increasing from 0.7 to 1.3 and 5.3, respectively. The relative share of wild ungulates in wolf diet was significantly higher in all three regions of wolf range in Croatia, even where livestock availability was high. Female wolves ate birds, rodents, and dogs more than males and in the south, where wild ungulates were scarce. This study showed wolves’ selectivity for wild ungulate, rather than for abundant, but well-guarded livestock. The European idea of coexistence of humans and wolves in human-dominated landscape seems possible with some effort and understanding from humans’ side.

Nursing Student’s Knowledge about Understanding and Prevention of Needle Stick Injury

Introduction. Needle stick injury (NSI) is an occupational health and safety issue. Nursing students are prone to NSI due to lack of experience with handling needles and sharps. Aim. To determine the level of knowledge about the prevention of NSI and examine the level of knowledge about the post-exposure procedure and the students’ understanding of NSI. Methods. The study was conducted in 2017 at the University of Applied Health Sciences in Zagreb. The participants were nursing students from all regions of Croatia. The data was collected using a questionnaire containing 17 questions specifically designed for this study. One question had three subquestions and one had five subquestions regarding the knowledge of how to react if a needle stick injury occurs. The participants also responded to a questionnaire on their socio-demographic data. Results. The study included 149 students. The results show that 16 students have experienced NSI. A statistically significant difference was observed among students who have finished a secondary medical school in the answers about post-NSI interventions and in answers to the question of whether the needle should be recapped. The respondents from medical schools answered correctly. A statistically significant difference was observed among students from non-medical secondary schools in the answers about education on post-exposition procedures and in the answers about necessary action following a needle stick injury. The respondents from non-medical secondary schools had higher scores. Conclusion. The results of this study can be used to establish appropriate education strategies, increase the awareness of needle stick injuries and minimize the occurrence of these injuries among nursing students in Croatia.

Demographic Characteristics of Chemistry Teachers in Croatia Affecting the Use of Pre-laboratory Activities in the Classroom

Pre-laboratory activities are designed to focus the attention of students on some aspects of the experiment they are preparing to do during the week. Previous research has found that such activities reduce the cognitive load in laboratory time and tend to increase the efficiency of students' laboratory work. This research aims at comparing the importance of demographic characteristics affecting the teachers' use of pre-laboratory activities in a chemistry class. In the frame of the quantitative survey research, an online questionnaire was completed by 166 chemistry teachers from all regions in Croatia. In pre-laboratory sessions, the teachers most commonly used a pre-lab discussion and pre-lab worksheets whereas computer simulations were represented the least. Three characteristics affecting the teachers' use of pre-laboratory activities in chemistry classes were their gender, age and teaching subjects. The teachers' education, teaching experience and school types were nonsignificant characteristics.

Aflatoxins and ochratoxin A in dry-fermented sausages in Croatia, by LC-MS/MS

ABSTRACT The aim of this study was to develop a highly-sensitive liquid chromatographic – tandem mass spectrometric (LC-MS/MS) method to investigate the presence of aflatoxins (AFB1, AFB2, AFG1 and AFG2) and ochratoxin A (OTA) in traditional homemade sausages (n = 88) collected from small family farms situated in different regions in Croatia. Aflatoxins were not detected (<LOD) in any of the analysed samples, whereas the percentage of OTA-positive homemade sausages was 14.8%, consisting of 1.1% Istrian sausages, 7.9% Slavonian sausages and 5.7% Kulenova Seka. In Slavonian sausages, the mean OTA concentration was 0.27 ± 0.09 μg/kg with a maximum of 0.38 μg/kg, while in Kulenova Seka the mean concentration was 0.26 ± 0.14 μg/kg, rising to a maximum of 0.48 μg/kg. Statistically significant differences (p = .243) in OTA concentrations across different types of homemade sausage and sampling regions were not determined.

Otpornost autohtonih sojeva Bradyrhizobium japonicum na stres izazvan nedostatkom vlage

Bioloska fiksacija dusika uvelike ovisi o uvjetima tla, a nedostatak vode uzrokuje porast koncentracije soli i povisenje osmotskog tlaka u tlu sto

The role of vocational education in developing entrepreneurial competences of students

Entrepreneurship is defined as one of key lifelong competences and it represents ability to turn ideas into real projects. As such, it includes creativity, initiative, taking responsibility, taking risks, planning and managing projects. The development of entrepreneurial competences via formal education has become a priority for governments of a large number of European countries. Many EU documents emphasize the importance of investing into education that focuses on the development of entrepreneurial competences, as it directly affects students’ employment. However, this guideline represents a new challenge for the educational system – the need to change educational programs and organize them so that they influence the development of entrepreneurial competences, which enables students to become more employable and to cope with uncertainty, complexity and dynamics of the labor market. High unemployment rate of young people and challenges and demands of the labor market have led to a debate about the effectiveness and efficiency of educational programs at all levels. In this context, effectiveness and quality of vocational education and training (VET) are particularly important, since most vocational school students, after finishing secondary level, will not continue towards tertiary education, but will rather find themselves on the labor market. Therefore, vocational education should focus on the development of such skills (generic competences and vocational qualifications) that will correspond to the needs of the labor market, thus enabling easier and faster employment. The main purpose of this paper is to determine the extent to which vocational education contributes to development of students’ entrepreneurial competences. The data used in this paper comes from a survey that covered 1272 students from 15 secondary vocational schools in Osijek-Baranja County, one of the most underdeveloped regions in Croatia. The analysis of the results has shown that there is a positive correlation between extracurricular activities and entrepreneurial environment with the level of students’ entrepreneurial competences. No correlation between the formal educational program and the level of entrepreneurial competences has been found. The findings of this research may serve as the basis for the development of vocational education programs that would include and meet the labor market demands, thus increasing students’ employability.

Comparison of PAH Mass Concentrations in Aerosols of the Middle Adriatic Coast Area and Central Croatia

Polycyclic aromatic hydrocarbons (PAHs) are considered priority pollutants due to their distribution, persistence, bioaccumulation, and adverse effects on human health. The aim of this study was to obtain the first insight into PAHs mass concentrations bound to airborne particulate matter (PM) in the mid Adriatic coast area of Croatia, and compare them with the PAH levels in Croatia’s urban central area. Relatively low values of PAH mass concentrations were measured in the coastal area, compared to the continental urban region of Croatia impacted by increased emissions due to intensive traffic, industry, and residential heating. A high PM10 contribution of four-ring PAHs (Flu and Pyr) at Martinska site indicated that wood burning heating as well as open fire events, including waste incineration, could be important emission sources of PAHs in the mid Adriatic coast region.