Reduced Graphene Oxide-silver Nanoparticle Research Articles

In Situ Synthesis, Characterization and Photocatalytic Efficacy of Silver-Enhanced MXene and Graphene Nanocomposites

The emergence of 2D materials has significantly expanded the wide range of nanomaterials with diverse applications. Notably, their high conductivity, catalytic efficiency, and hydrophobicity have fueled heightened research interests for water treatment applications. This research aimed to investigate the synthesis and characterization of MXene and reduced graphene oxide (rGO) nanocomposites with silver nanoparticles (Ag) for enhanced catalytic activity in the decomposition of Direct Blue-24 dye. In this study, we employed well-established methods, previously documented in the literature, to prepare two distinct nanocomposites. Novel nanocomposites, namely reduced graphene oxide–silver nanoparticles (rGO–Ag) and MXene–silver nanoparticles (MXene–Ag), were synthesized using the hydrothermal and direct reduction method with an ammoniacal solution (aqueous solution). Comprehensive characterization using advanced tools revealed that the introduced Ag particles integrated seamlessly onto the parent nanofilms of the Carbon derivatives, forming a secondary phase with enhanced catalytic functionality. These nanocomposites demonstrated significant improvements in the catalytic decomposition reactions in simulated wastewater. Verification involved the reduction reaction of Direct Blue-24 dye at known nanocomposite concentrations. The results indicated that MXene–Ag exhibited a superior catalytic activity of 98% in 10 min compared to the rGO–Ag nanocomposite films, which achieved 96% in 35 min. The results indicated that MXene–Ag nanocomposites exhibited a 20–25% increase in catalytic efficiency compared to the rGO–Ag nanocomposites. The outcomes of this research hold promise for practical applications in textile wastewater management and various industrial sectors dealing with mutagenic and carcinogenic chemicals containing azo and/or phthalocyanine products.

Synthesis of nanocomposites reduced graphene oxide-silver nanoparticles prepared by hydrothermal technique using sodium borohydride as a reductor for photocatalytic degradation of Pb ions in aqueous solution

Heavy metals are pollutants that are harmful to living things and the environment can be degraded by microbes or understood by other living things so that they can cause health problems. One of the heavy metals that is often found in wastewater is lead. Lead is widely used in the manufacture of batteries, metal products such as ammunition, cable coatings, Polyvinyl Chloride (PVC) tubing, solder, chemicals and dyes This use causes humans to be exposed to large amounts of lead. One method to deal with lead pollution is to use photocatalysts. Photocatalysts react with heavy metals and reduce them so that the level of toxicity becomes lower than before through photocatalytic reactions. In this study, synthesis of reduced graphene oxide/silver nanoparticle nanoparticles was performed by facile hydrothermal methods for photocatalytic degradation of Pb ion. The characterization results indicate that the synthesis has been successfully carried out. The successful result of rGO/AgNPs nanocomposites synthesis was proved by several techniques such as X-ray diffraction analysis, Raman, UV-Vis spectroscopy, Scanning Electron Microscopy (SEM) and Energy Dispersive X-Ray analysis (EDX). This indicates the presence of these groups in the graphene oxide and rGO/AgNPs samples, respectively. The resulting rGO/AgNPs nanocomposite has an absorbance peak at a wavelength of 267 nm. The diffraction peaks for nanocomposites rGO/AgNPs and their Miller indices were 38.08° (111), 44.16° (200), 64.44° (220), and 77.44° (311). The Raman spectra of rGO/AgNPs exhibits D bands at 1334,13 with intensity of 630,60 cm−1 and G band at 1594,61 with intensity of 477,29 cm−1. The ID/IG ratio rGO/AgNPs-NaBH4 is ~1,32. Furthermore, the photocatalytic activity test results showed that the rGO/AgNPs nanocomposite was able to reduce Pb2+ to Pb with a maximum exposure time of 1.5 hours

Reduced Graphene Oxide-Silver Nanoparticles for Optical Pulse Generation in Ytterbium- and Erbium-Doped Fiber Lasers

This work has demonstrated the potential of a reduced graphene oxide silver/polyvinyl alcohol (rGO-Ag/PVA) film as a saturable absorber (SA) in ytterbium and erbium based Q-switched optical fiber lasers. The facile hydrothermal method was used to synthesize the nanocomposite between rGO and Ag nanoparticles. This was followed by a simple solution method to form the rGO-Ag film using PVA as the host polymer. From nonlinear absorption characterization, the rGO-Ag/PVA SA was determined to have a modulation depth of 30%, a nonsaturable loss of 70%, and a saturable intensity of 0.63 kW/cm2. Stable self-starting Q-switched pulses were obtained at the threshold pump power of 72.76 mW and 18.63 mW in the ytterbium-doped (YDFL) and erbium-doped fiber laser (EDFL) cavities respectively. The center operating wavelengths were observed at 1044.4 nm and 1560 nm for the two cavities. The shortest pulse width and maximum repetition rate of the YDFL and EDFL were 1.10 µs and 62.10 kHz and 1.38 µs and 76.63 kHz respectively. This work has demonstrated that the rGO-Ag/PVA film is suitable as an SA for pulse generation in the 1.0 and 1.5 μm regions and would have many potential photonics applications.

Novel electrochemical aptasensor with dual signal amplification strategy for detection of acetamiprid

In this work, a novel dual signal amplification strategy for aptasensor employing reduced graphene with silver nanoparticles and prussian blue-gold nanocomposites was developed for detection of acetamiprid. To improve the sensitivity of aptasensors, reduced graphene oxide-silver nanoparticles (rGo-AgNPs) were modified on a bare glassy carbon electrode surface, which provided a large specific surface area for subsequent material immobilization and amplified current signal. The electrical signal output and sensitivity of the aptasensor was significantly improved after the immobilization of prussian blue-gold nanoparticles (PB-AuNPs) as a catalyst for the redox reaction. The analysis experiment exhibited that it had super-high sensitivity with a detection limit of 0.30 pM (S/N = 3), which met the requirements of the vast majority of daily leaf vegetable testing. Under optimized conditions, the proposed aptasensor showed a wide linear detection range from 1 pM to 1 μM. This aptasensor also had good stability and high selectivity for acetamiprid detection without an interfering effect of some other pesticides. The proposed aptasensor displayed good recovery rates in real samples, which proposed a new method for constructing electrochemical sensors and provided a novel tool for rapid, sensitive analysis of pesticides with low cost.

Green synthesized rGO-AgNP hybrid nanocomposite – An effective antibacterial adsorbent for photocatalytic removal of DB-14 dye from aqueous solution

In this study, a facial synthesis of a reduced graphene oxidesilver nanoparticle hybrid nanocomposite (rGO-AgNP) was carried out from aqueous extract of Brassica nigra by the reduction of graphene oxide and silver ions simultaneously. The synthesized nanocomposite was characterized using UV–vis spectroscopy, X-ray diffraction, scanning electron microscope, energy dispersive x-ray spectroscopy, and Fourier transform infrared spectroscopy. The antibacterial activity examination of rGO-AgNP nanocomposite against both gram negative and gram positive bacterial strains ensured enhanced antibacterial effect. The phytosynthesized nanocomposite acts as a photocatalytic agent for the removal of dye thereby finding its way in water treatment. Direct blue-14 (DB-14) dye was used to study the adsorption efficiency of synthesized nanocomposites. The parameters that influence the rate of adsorption such as effect of time, pH, light source and adsorbent dosage were investigated. The perfect recovery of adsorbent after reaction and its unchanged efficiency for cyclic use showed that it will be an economically and environmentally friendly photocatalyst. Isotherm models like Langmuir and Freundlich were used to assess the capability of adsorbent on adsorption. The outcomes show that the rGO-AgNP composites are effectual in adsorbing dye and have a promising effect on water treatment.

Fabrication of Reduced Graphene Oxide-Ag Nanocomposites and Analysis on the Interaction with BSA

Graphene is an excellent platform to support and stabilize silver nanoparticles (AgNPs). The reduced graphene oxide-silver nanoparticles (rGO-AgNPs) were synthesized by the chemical reduction method and characterized by using ultraviolet-visible (UV-vis) absorption, transmission electron microscopy (TEM), X-ray diffractometer (XRD), and scanning probe microscopy (SPM). The binding reaction of rGO-AgNPs with bovine serum albumin (BSA) was investigated by using fluorescence spectrometry and SPM. As the concentration of AgNPs increased, the fluorescence spectrum was quenched, and the quenching process of rGO-AgNPs and BSA was static quenching. Thermodynamic parameters of the absorption process were evaluated at different temperatures, and the negative values of Gibbs free energy ( Δ G ) showed that this process was spontaneous. The main type of interaction was hydrophobic interaction according to the values of changes in standard enthalpy ( Δ H ) and entropy ( Δ S ). In addition, the morphology changes of proteins interacting with nanomaterials were detected by SPM.

Electrochemical detection of L-dopa using crude Polyphenol oxidase enzyme immobilized on electrochemically reduced RGO-Ag nanocomposite modified graphite electrode

In the present work, we report the selective and sensitive electrochemical detection of L-dopa using crude Polyphenol oxidase enzyme (PPO) immobilized on electrochemically reduced graphene oxide–silver nanoparticles (RGO-Ag) nano composite modified graphite (Gr) electrode. The Gr electrode is first modified through the electrochemical reduction of graphene oxide - silver (GO-Ag) nano composite to RGO-Ag nano composite on graphite electrode. The crude extract of PPO from Manilkara Zapota (sapota) fruit is later immobilized onto Gr/RGO-Ag modified electrode. Modification of Gr electrode at each step is confirmed by cyclic voltammetry (CV) and Electrochemical impedance (EIS) techniques. The surface characterization of modified electrode is performed by Scanning electron microscopy (SEM). The developed sensor exhibited excellent electrocatalytic activity towards the detection of L-dopa in the concentration range of 1–150 µM with low detection limit and high sensitivity. Further, the practical applicability of the developed biosensor is examined for the detection of L-dopa in urine samples.

Graphene Oxide-Silver Nanocomposite Enhances Cytotoxic and Apoptotic Potential of Salinomycin in Human Ovarian Cancer Stem Cells (OvCSCs): A Novel Approach for Cancer Therapy.

The use of graphene to target and eliminate cancer stem cells (CSCs) is an alternative approach to conventional chemotherapy. We show the biomolecule-mediated synthesis of reduced graphene oxide–silver nanoparticle nanocomposites (rGO–Ag) using R-phycoerythrin (RPE); the resulting RPE–rGO–Ag was evaluated in human ovarian cancer cells and ovarian cancer stem cells (OvCSCs). The synthesized RPE–rGO–Ag nanocomposite (referred to as rGO–Ag) was characterized using various analytical techniques. rGO–Ag showed significant toxicity towards both ovarian cancer cells and OvCSCs. After 3 weeks of incubating OvCSCs with rGO–Ag, the number of A2780 and ALDH+CD133+ colonies was significantly reduced. rGO–Ag was toxic to OvCSCs and reduced cell viability by mediating the generation of reactive oxygen species, leakage of lactate dehydrogenase, reduced mitochondrial membrane potential, and enhanced expression of apoptotic genes, leading to mitochondrial dysfunction and possibly triggering apoptosis. rGO–Ag showed significant cytotoxic potential towards highly tumorigenic ALDH+CD133+ cells. The combination of rGO–Ag and salinomycin induced 5-fold higher levels of apoptosis than each treatment alone. A combination of rGO–Ag and salinomycin at very low concentrations may be suitable for selectively killing OvCSCs and sensitizing tumor cells. rGO–Ag may be a novel nano-therapeutic molecule for specific targeting of highly tumorigenic ALDH+CD133+ cells and eliminating CSCs. This study highlights the potential for targeted therapy of tumor-initiating cells.

Reduced graphene oxide-silver nanoparticles/nitrogen-doped carbon nanofiber composites with meso-microporous structure for high-performance symmetric supercapacitor application

The electrochemical performance of carbon nanofibers (CNFs) as the electrode material of the supercapacitor should be improved to meet requirements of renewable energy systems. We report here the synthesis of nitrogen-doped carbon nanofibers (RGO-AgNP/N-CNF) with a meso-microporous structure by the supplement of reduced graphene oxide–silver nanoparticles (RGO-AgNPs) into N-CNFs. The usage of polyacrylonitrile (PAN) is aimed to supply nitrogen doped in carbon bulk, which can enhance fast migration of electrolyte ions during the electrochemical process. Decoration of AgNPs on RGO surface improves electrical conductivity of RGO-AgNP/N-CNF composites and alleviates the aggregation of AgNPs. The addition of RGO-AgNPs elevates the specific surface area and mesoporous content of CNFs. The assembled RGO-AgNP/N-CNF symmetric supercapacitor possesses high specific capacitance of 188.0 F g−1 at a current density of 0.5 A g−1, delivers an ultrahigh energy density of 26.1 Wh kg−1 and displays an excellent cycling performance (97.5% retention after 5000 cycles), which suggest its big potential as energy storage devices.

Novel biomolecule lycopene-reduced graphene oxide-silver nanoparticle enhances apoptotic potential of trichostatin A in human ovarian cancer cells (SKOV3).

BackgroundRecently, there has been much interest in the field of nanomedicine to improve prevention, diagnosis, and treatment. Combination therapy seems to be most effective when two different molecules that work by different mechanisms are combined at low dose, thereby decreasing the possibility of drug resistance and occurrence of unbearable side effects. Based on this consideration, the study was designed to investigate the combination effect of reduced graphene oxide-silver nanoparticles (rGO-AgNPs) and trichostatin A (TSA) in human ovarian cancer cells (SKOV3).MethodsThe rGO-AgNPs were synthesized using a biomolecule called lycopene, and the resultant product was characterized by various analytical techniques. The combination effect of rGO-Ag and TSA was investigated in SKOV3 cells using various cellular assays such as cell viability, cytotoxicity, and immunofluorescence analysis.ResultsAgNPs were uniformly distributed on the surface of graphene sheet with an average size between 10 and 50 nm. rGO-Ag and TSA were found to inhibit cell viability in a dose-dependent manner. The combination of rGO-Ag and TSA at low concentration showed a significant effect on cell viability, and increased cytotoxicity by increasing the level of malondialdehyde and decreasing the level of glutathione, and also causing mitochondrial dysfunction. Furthermore, the combination of rGO-Ag and TSA had a more pronounced effect on DNA fragmentation and double-strand breaks, and eventually induced apoptosis.ConclusionThis study is the first to report that the combination of rGO-Ag and TSA can cause potential cytotoxicity and also induce significantly greater cell death compared to either rGO-Ag alone or TSA alone in SKOV3 cells by various mechanisms including reactive oxygen species generation, mitochondrial dysfunction, and DNA damage. Therefore, this combination chemotherapy could be possibly used in advanced cancers that are not suitable for radiation therapy or surgical treatment and facilitate overcoming tumor resistance and disease progression.

Combination of graphene oxide-silver nanoparticle nanocomposites and cisplatin enhances apoptosis and autophagy in human cervical cancer cells.

BackgroundCisplatin (Cis) is a widely used chemotherapeutic drug for treating a variety of cancers, due to its ability to induce cell death in cancer cells significantly. Recently, graphene and its modified nanocomposites have gained much interest in cancer therapy, due to their unique physicochemical properties. The objective of this study was to investigate the combination effect of Cis and a reduced graphene oxide–silver nanoparticle nanocomposite (rGO-AgNPs) in human cervical cancer (HeLa) cells.Materials and methodsWe synthesized AgNPs, rGO, and rGO-AgNP nanocomposites using C-phycocyanin. The synthesized nanomaterials were characterized using various analytical techniques. The anticancer properties of the Cis, rGO-AgNPs, and combination of Cis and rGO-AgNPs were evaluated using a series of cellular assays, such as cell viability, cell proliferation, LDH leakage, reactive oxygen species generation, and cellular levels of oxidative and antioxidative stress markers such as malondialdehyde, glutathione, SOD, and CAT. The expression of proapoptotic, antiapoptotic, and autophagy genes were measured using real-time reverse-transcription polymerase chain reaction.ResultsThe synthesized AgNPs were well dispersed, homogeneous, and spherical, with an average size of 10 nm and uniformly distributed on graphene sheets. Cis, GO, rGO, AgNPs, and rGO-AgNPs inhibited cell viability in a dose-dependent manner. The combination of Cis and rGO-AgNPs showed significant effects on cell proliferation, cytotoxicity, and apoptosis. The combination of Cis and rGO-AgNPs had more pronounced effects on the expression of apoptotic and autophagy genes, and also significantly induced the accumulation of autophagosomes and autophagolysosomes, which was associated with the generation of reactive oxygen species.ConclusionOur findings substantiated rGO-AgNPs strongly potentiating Cis-induced cytotoxicity, apoptosis, and autophagy in HeLa cells, and hence rGO-AgNPs could be potentially applied to cervical cancer treatment as a powerful synergistic agent with Cis or any other chemotherapeutic agents.

Prepare poly-dopamine coated graphene@silver nanohybrid for improved surface enhanced Raman scattering detection of dyes

A novel hybrid of reduced graphene oxide-silver nanoparticle composite (rGO-Ag) wrapped by a thin layer of polydopamine (PDA) (rGO-Ag@PDA) was prepared as a highly active substrate for surface enhanced Raman scattering detection of aromatic dyes. rGO-Ag prepared by one-pot citrate reduction was coated with PDA through the self-polymerization of dopamine to produce the rGO-Ag@PDA hybrid. The morphology, component, absorption ability and SERS activity of the products were characterized by SEM, EDS, FTIR, UV–vis absorption, and Raman spectroscopy. Due to the PDA coating, the rGO-Ag@PDA hybrid shows enhanced adsorption ability to the dye molecules, and subsequently leads to stronger SERS signal of dyes than that obtained on widely reported rGO-Ag hybrid substrate. SERS detection of crystal violet, methylene blue, and malachite green on the rGO-Ag@PDA can be down to 0.1nM, 0.1μM and 1nM, respectively. Moreover, the rGO-Ag@PDA also displayed enhanced stability and improved recyclability than those of rGO-Ag. The proposed substrate may be used for SERS analysis and environmental monitoring.

Terminalia bellirica mediated one-pot green synthesis of reduced graphene oxide-silver nanoparticle (RGO/AgNP) nanocomposite and its catalytic activity towards p-nitrophenol reduction in water

Terminalia bellirica mediated one-pot green synthesis of reduced graphene oxide-silver nanoparticle (RGO/AgNP) nanocomposite and its catalytic activity towards p-nitrophenol reduction in water

Green Synthesis of Reduced Graphene Oxide-Silver Nanoparticles Using Environmentally Friendly L-arginine for H2O2 Detection

The paper reports on the preparation of reduced graphene oxide/silver nanoparticles (rGO/AgNPs) nanocomposite through the reduction of graphene oxide (GO) and silver nitrate (AgNO3) using environmentally friendly L-arginine (Arg). The chemical composition of the resulting nanocomposite has been analyzed using transmission infrared (FTIR) spectroscopy, Raman spectroscopy and X-ray photoelectron spectroscopy (XPS). The results indicated that Arg was incorporated in the rGO matrix. The morphology of the rGO/Arg/AgNPs composite has been characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Ag NPs with an average diameter of 20 nm are homogeneously distributed on crumpled graphene nanosheets. The electrocatalytic activity of the rGO/Arg/AgNPs hybrid nanomaterial has been investigated for the nonenzymatic detection of hydrogen peroxide (H2O2) in PBS solution. The sensor displayed a detection limit of ≈50 μM over a wide linear range from 0.2 to 2.5 mM with a sensitivity of 1.34 mA mM−1 cm−2. In addition, the sensor also showed good selectivity for H2O2 detection and long-term stability.

Dissolved oxygen detection by galvanic displacement-induced graphene/silver nanocomposite

This paper proposed a simple, efficient and sensitive electrochemical sensor for dissolved oxygen (DO) detection based on a galvanic displacement synthesized reduced graphene oxide–silver nanoparticles (RGO/Ag) composite modified grassy carbon electrode (GCE). The synthesized RGO/Ag nanocomposite was characterized by UV–vis spectroscopy, Raman spectroscopy, scanning electron microscopy (SEM) and X-ray diffraction (XRD). The results indicate the graphene oxide (GO) has been successfully reduced during the galvanic displacement process and the average size of Ag nanoparticle is 52 nm. The RGO/Ag nanocomposite-modified GCE showed a significant enhancement of DO detection compared with bare and RGO-modified GCEs. Moreover, the proposed DO sensor also exhibited an excellent repeatability, reproducibility and anti-interference ability.

A facile fabrication of large-scale reduced graphene oxide–silver nanoparticle hybrid film as a highly active surface-enhanced Raman scattering substrate

Larger-scale reduced graphene oxide–silver nanoparticle hybrid film can serve as a novel SERS-active substrate for detecting R6G and MA.

Reduced graphene oxide-silver nanoparticle composite as visible light photocatalyst for degradation of colorless endocrine disruptors.

Sunlight-induced degradation of organic pollutants is an ideal approach for environmental pollution control and wastewater treatment. Although a variety of photocatalysts have been designed toward this goal, efficient degradation of colorless organic pollutants by visible light is a challenging issue. Here, we show that a reduced graphene oxide (rGO)-based composite with silver nanoparticle (rGO-Ag) can act as an efficient visible-light photocatalyst for the degradation of colorless organic pollutants. We have developed a simple, large-scale synthesis method for rGO-Ag and used it for the degradation of three well-known endocrine disruptors (phenol, bisphenol A, and atrazine) under UV and visible light. It is found that photocatalytic efficiency by rGO-Ag under visible light is significantly higher compared to that of rGO or silver nanoparticles. It is proposed that Ag nanoparticles offer visible-light-induced excitation of silver plasmons, and conductive rGO offers efficient charge separation and thus induces oxidative degradation of the organic pollutant. This approach can be extended for sunlight-induced degradation of different organic pollutants.

Preparation and Electrocatalytic Properties of Polydopamine Functionalized Reduced Graphene Oxide-Silver Nanocomposites

Polydopamine functionalized reduced graphene oxide-silver nanoparticle (PDA-RGO/Ag NP) nanocomposites were successfully prepared by a simple and mild procedure. Graphene oxide (GO) sheets were firstly coated with PDA via a self-polymerization process which provided an excellent interface for in-situ growing silver nanoparticles. Fourier transform infrared spectroscopy (FTIR) confirmed the successful coating of PDA and informed the reduction of the surface functional groups of GO. The formation of reduced GO and silver NPs was further evidenced by UV-Vis and X-ray diffraction spectroscopy. The as-prepared PDA-RGO/Ag nanocomposites could greatly enhance the electrochemical reduction of hydrogen peroxide (H2O2). This excellent performance was attributed to the increased effective electrode surface area due to the deposition of nano-sized Ag particles and graphene. The PDA-RGO/Ag-based electrochemical sensor displayed a rapid amperometric response for H2O2 measurement with a wide linear range from 0.5 μM to 8 mM and a low detection limit of 2.07 μM.

Identifying efficient natural bioreductants for the preparation of graphene and graphene-metal nanoparticle hybrids with enhanced catalytic activity from graphite oxide

The implementation of green approaches towards the preparation of graphene and graphene-based materials with enhanced functionality from graphite oxide has been relatively little explored. Particularly, the use of bioreductants and the testing of their relative efficacies is an incipient area of research. Here, a pool of 20 environmentally friendly, natural antioxidants have been tested for their ability to reduce graphene oxide. These antioxidants were mostly vitamins, amino acids and organic acids. By establishing a protocol to systematically compare and optimize their performance, several new efficient bioreductants of graphene oxide have been identified, namely, pyridoxine and pyridoxamine (vitamin B6), riboflavin (vitamin B2), as well as the amino acids arginine, histidine and tryptophan. These biomolecules were used to prepare reduced graphene oxide–silver nanoparticle hybrids that displayed colloidal stability in water in the absence of additional dispersants. Particularly, hybrids prepared with pyridoxamine exhibited a combination of long-term colloidal stability and exceptionally high catalytic activity among silver nanoparticle-based catalysts in the reduction of p-nitrophenol with NaBH4. Thus, in addition to expanding substantially the number of green reductants available for graphene oxide reduction, the present results underline the idea that proper selection of bioreductant can be relevant to achieve graphene-based materials with improved performance.

Reduced Graphene Oxide–Silver Nanoparticle Composite as an Active SERS Material

Selectivity and enhanced sensitivity for SERS measurements are highly desirable for environmental and analytical applications. Interaction of a target molecule with SERS substrate plays a pivotal role in determining the magnitude of enhancement and spectral profile of the SERS signal. A reduced graphene oxide–Ag nanoparticle (RGO-Ag NP) composite has been designed to boost SERRS sensitivity of a porphyrin derivative. Complexation between 5,10,15,20-tetrakis(1-methyl-4-pyridinio)porphyrin tetra(p-toluenesulfonate) (TMPyP) porphyrin and the RGO-Ag NP composite is evidenced by a red-shifted porphyrin absorption band. Results indicate complexation is influential in improved surface-enhanced resonance Raman (SERRS) signal for TMPyP and thus offers an advantage for target molecule detection at low concentration levels. The combined effects of RGO and Ag NPs in the enhancement of SERS signal of TMPyP are discussed.