Memory Recovery Research Articles

Effects of energized treatment on electro trigger shape memory behavior

The shape memory polymers (SMPs) can be programmed to take temporary shapes and return to their original shape when subjected to external stimuli. Electrically triggered SMP has become an important branch of shape memory materials because of its advantages such as remote heating, fast response and local control. In this paper, a novel shape memory composite structure is prepared by using polylactic acid (PLA) as substrate and short carbon fiber (SCF) as conductive filler. The electrification treatment during the preparation process has a beneficial effect on the SCF arrangement. Compared with the sample without electrification treatment, the highest conductivity can be increased from 43.96 S/m to 66.67 S/m, and the performance is improved by 51.66 %. When the voltage is increased from 3 V to 8 V, the time to reach the glass transition temperature (Tg) is reduced by 62.22 %, and the shape memory recovery time is reduced by 53.68 %. The intelligent fixture based on the composite structure can realize the remote grasp of 3D printed balls with a diameter of 40 mm and a weight of 18.35 g under pressure, which fully proves the reliable electrically triggered shape memory performance of the composite structure and the great application potential in the field of robot gripper.

The study on the properties of a metamaterial composed of star-shaped auxetic and curved-beam multi-stable structure

This article presents the design of a new metamaterial with adjustable Poisson’s ratio and multi-stability, which combines two common metamaterial structures: star-shaped auxetic and multi-stability. The range of negative Poisson’s ratio variation for the unit structure is 0.2433. The negative Poisson’s ratio shows an increasing trend followed by a decrease with the increase in compression displacement. The peak compression displacement is 12 mm, and the peak negative Poisson’s ratio is −0.1499. Simultaneously, the structure exhibits good shape memory properties, with a shape memory recovery ratio of R rx = 94.18% in the y-axis direction and R ry = 91.20% in the x-axis direction. The shape memory recovery time is 35.10 s. The combination of experimental testing and finite element analysis was employed to verify the functionality of the adjustable Poisson’s ratio in the structure. Special attention was given to investigating the structural parameters such as the type of curved-beam, the angle of the star-shaped structure, the width of the star-shaped structure, and the connection method for their effects on the shape memory effect and the range, peak value, and peak compression displacement of the negative Poisson’s ratio in the unit structure. Based on the characteristics of the novel metamaterial, including a significant increase in stiffness upon compression and controllable deformation sequence, it can be used as a buffering device in protective engineering and civil engineering applications.

Relationship between personal recovery, autobiographical memory, and clinical recovery in people with mental illness in the acute phase

AimNarratives are important in psychiatric rehabilitation. People with a psychiatric diagnosis find it difficult to recall specific autobiographical memories of events that lasted less than a day. Although personal narratives play a central role in personal recovery, the factors influencing personal and clinical recovery, such as psychiatric symptoms and cognitive function, have not been fully explored. Therefore, this study examined the associations between personal recovery and autobiographical memory, age, psychiatric symptoms, and neurocognitive function. MethodThe Self-Identified Stage of Recovery, Parts A and B (SISR-A, SISR-B), Autobiographical Memory Test (AMT), Japanese version of the Brief Assessment Scale of Schizophrenia Cognitive Function, and Brief Psychiatric Symptom Rating Scale were administered to 40 individuals with psychiatric disorders who were undergoing psychiatric rehabilitation. ResultsA significant positive correlation was found between the total number of specific memories in the AMT and total SISR-B scores. A binary logistic regression analysis revealed that the total number of specific memories, especially high responsiveness to negative cue words, significantly predicted greater personal recovery. Age, psychiatric symptoms, and neurocognitive function did not significantly predict higher personal recovery. ConclusionIn psychiatric rehabilitation, negative episodes should be treated with caution; however, they may also facilitate personal recovery.

Recovered memories in psychotherapy: a survey of practicing psychotherapists in Germany

ABSTRACT We report on a survey of 258 psychotherapists from Germany, focusing on their experiences with memory recovery in general, suggestive therapy procedures, evaluations of recovered memories, and memory recovery in training and guidelines. Most therapists (78%) reported instances of memory recovery encompassing negative and positive childhood experiences, but usually in a minority of patients. Also, most therapists (82%) reported to have held assumptions about unremembered trauma. Patients who held these beliefs were reported by 83% of the therapists. Both therapist and patient assumptions reportedly occurred in a minority of cases. Furthermore, 35% of participants had used therapeutic techniques at least once to recover presumed trauma memories. Only 10% reported assuming trauma in most patients and recovering purported memories in a majority of the attempts. A fifth believed memory recovery was a task of psychotherapy. This belief correlated with trauma assumptions, memory recovery attempts, and recovery frequency. Psychodynamic therapists more often reported to assume trauma behind symptoms and agreed more with problematic views on trauma and memory. No differences showed regarding suggestive behaviour in therapy. Most participants expressed interest in receiving support on dealing with memory recoveries. This interest should be taken up, ideally during therapist training.

Enhancing mechanical and shape memory properties of polylactide/polyamide elastomer blends via reactive blending with a chain extender

AbstractThe structure and properties of incompatible polylactide (PLA)/polyamide elastomer (PAE) blends were tailored by a chain extender specifically the styrene–glycidyl acrylate copolymer Joncryl ADR4368 (ADR). Various PLA/PAE/ADR blends with different compositions were prepared by melt blending, and their morphology, crystallization behavior, and mechanical and the shape memory properties were systematically investigated. The results showed a uniform dispersion of PAE particles in the PLA matrix for the PLA blends with a reduction in particle size upon the addition of ADR. The crystallization of PLA was retarded, which was confirmed by a decrease in the melt crystallization temperature and an increase in cold crystallization temperature in the PLA/PAE/ADR blends. Rheological analysis showed an improvement in the melt elasticity of the PLA/PAE binary blend due to the presence of ADR, possibly attributed to the formation of long‐chain‐branched copolymers at the interface. Notably, the PLA/PAE/ADR blend exhibited superior toughness, featuring an elongation at break of 288% and a notched impact strength of 37 kJ·m−2, along with a high shape memory fixation rate and recovery rate when the ADR content was 1 wt%. Furthermore, the underlying toughening mechanism was elucidated. This work may offer an industrially scalable relevant model to fabricate high‐performance PLA materials.

Targeting pyroptosis with nanoparticles to alleviate neuroinflammatory for preventing secondary damage following traumatic brain injury.

Posttraumatic neuroinflammation is a key driver of secondary injury after traumatic brain injury (TBI). Pyroptosis, a proinflammatory form of programmed cell death, considerably activates strong neuroinflammation and amplifies the inflammatory response by releasing inflammatory contents. Therefore, treatments targeting pyroptosis may have beneficial effects on the treatment of secondary brain damage after TBI. Here, a cysteine-alanine-glutamine-lysine peptide-modified β-lactoglobulin (β-LG) nanoparticle was constructed to deliver disulfiram (DSF), C-β-LG/DSF, to inhibit pyroptosis and decrease neuroinflammation, thereby preventing TBI-induced secondary injury. In the post-TBI mice model, C-β-LG/DSF selectively targets the injured brain, increases DSF accumulation, and extends the time of the systemic circulation of DSF. C-β-LG/DSF can alleviate brain edema and inflammatory response, inhibit secondary brain injury, promote learning, and improve memory recovery in mice after trauma. Therefore, this study likely provided a potential approach for reducing the secondary spread of TBI.

La transmisión transgeneracional de situaciones traumáticas: papel de la psicología en el proceso de recuperación de la memoria colectiva de la dictadura franquista en España

In this work, some considerations are made about memory and forgetting, complex individual and family emotional processes that give rise to certain collective psychological states that are usually configured under dictatorships and totalitarian states are described. These reflections are framed in the context of Spain's recent history and will help to understand, at least in part, the enormous delay that the recovery of memory has had in our country. These contributions are supported by historical, sociological, and literary sources and based on psychological theory and clinical practice applied to individual, family, and group treatments.

Utilizing Music Therapy for Enhanced Recovery from Neurologic Disease Complications

Neurologic music therapy (NMT) has proven instrumental in aiding recovery from complications in patients with a diverse range of neurologic diseases. Integrating music and virtual reality with standard rehabilitation therapies enhances patient compliance and makes therapy more enjoyable. The act of listening to music not only reduces epileptiform discharges but also amplifies brain plasticity. Moreover, music induces discernible variations in brain anatomy between musicians and non-musicians. As a cost-effective intervention, music therapy significantly contributes to the accelerated and efficient recovery of post-stroke patients, particularly when applied promptly after the event. Substantial evidence supports the integration of music into rehabilitation programs, facilitating the recovery of hand function, dexterity, spatial movement, cognitive function, mood, coordination, stride length, and memory. Techniques such as learning words as lyrics, melodic intonation therapy, and singing play crucial roles in expediting recovery for aphasic patients. Recognized by the World Rehabilitation Federation, NMT therapists are valuable members of the rehabilitation team. The approval of NMT as an effective, evidence-based treatment method underscores its significance in enhancing patient outcomes.

Effect of thermomechanical training on recovery stress of Fe-based shape memory alloy

This research explores the impact of thermomechanical treatment on the recovery stress induced by the shape memory effect in an Fe-based shape memory alloy with the composition of Fe-17Mn-5Si-10Cr-4Ni-1(V,C) wt.%. Fe-based shape memory alloys have gained considerable attention for their potential applications in vibration control, energy dissipation, and structural strengthening or re-centering within the construction industry. To evaluate the recovery stress behaviour of this Fe-based shape memory alloy, specimens underwent a two-step aging heat treatment process, involving initial aging at 600 °C for 20 hours, followed by subsequent aging at 680 °C for 8 hours. Then heat-treated samples subjected to a cyclic thermomechanical treatment, involving a combination of deformation up to 2% and heating at 200 °C for 30 min in each cycle. The microstructure and mechanical properties were assessed using techniques such as scanning electron microscopy, as well as tensile tests. The results revealed that the thermomechanical treatment had a notable impact on the microstructure and mechanical properties of the Fe-based shape memory alloy. It resulted in an enhancement of the shape memory effect and recovery stress. These improvements were associated with an increase in the martensitic phase fraction. Additionally, the presence of VC precipitates in the treated samples contributed to the enhancement of the shape memory effect.

Therapeutic potential of Simvastatin and Doxycycline combination for Alzheimer’s disease

Alzheimer’s disease (AD) is a progressive neurodegenerative brain disorder resulting from Amyloid β (Aβ) accumulation. It leads to a decrease in memory and an increase in the production of reactive oxygen species and reactive nitrogen species and also increases Tubulin-associated unit phosphorylation. During AD, the cholesterol levels in the brain increase, and these regions are called lipid rafts. These lipid rafts are hosts for β and γ secretases. As a result, mono fibrils develop, which leads to Aβ oligomerization and, eventually, Aβ accumulation. Hence, the present study was designed to evaluate the protective effect of Simvastatin (cholesterol synthesis inhibitor) and Doxycycline (anti-amyloidogenic drug) for Aβ clearance in an Aβ-induced AD model, in female C57BL/6 mice. The mice were evaluated for spatial memory and olfactory sensation using the Morris water maze and Buried pellet test, respectively. The group treated with the combination of Simvastatin and Doxycycline at two different concentrations showed a recovery of memory and olfactory sensation when compared to the control group. The results have shown that the high-dose combination of Simvastatin and Doxycycline exhibits cholesterol lowering property by blocking the 3-hydroxy-3-methylglutaryl coenzyme A reductase pathway, thereby regulating cholesterol, and eventually resulting in anti-Alzheimer’s activity.

Collective Memory in the Diaspora as Represented in Crossing the River by Caryl Phillips and Small Island by Andrea Levy

The current study seeks to investigate how Crossing the River (1993) by Caryl Phillips and Small Island (2004) by Andrea Levy engage with what is known today as postcolonial counter-discourse to show how colonialism, slavery and racism shape the collective memory of African and Afro-Caribbean diaspora. It is also significant to understand how such a process allows both writers to use tangible or intangible forms of collective memory as tools in representing, reassessing and documenting the diaspora history from the perspective of the oppressed other. Moreover, a chance will be given to understand how they abrogate/dismantle essentialist hegemonic Western assumptions through collective forms of remembrance in multicultural contexts. In that sense, the analysis will draw on the concept of collective memory as defined by Maurice Halbuach, Aleida Assmann and Linda Shortt's description of memory as a powerful agent of change and Pierre Nora's sites of memory to explore how the diaspora characters remember or deal with their colonial past and its aftermath beyond their national borders. The study concludes that the process of memory recovery and transmission from one diasporic generation to the other, as representative subjects of multiple movements and dwellings, resulted in acquiring problematic senses of belonging due, in large part, to the pervasiveness of ethnic and racial oppression in the new world. At this stage, each diasporic member would consider such traumatic social environments as their premise to define and select what should be remembered or forgotten from a past marked with constant transformations. This selectivity is triggered by their impossible return to a place once called home in their collective diasporic consciousness.

Effect of Cerium Addition on the Microstructure and Shape Memory Properties of Austenitic Fe–Mn–Si–Cr–Ni Alloys

The addition of rare earth elements, such as cerium, to austenitic Fe–Mn–Si‐based shape memory alloys has been shown to improve both corrosion resistance and shape recovery. However, the mechanisms underlying the effect of Ce on shape recovery are still unclear. This study investigates the influence of the addition of small amounts of Ce (0.18, 0.42, and 0.96 wt%) on the microstructure and shape recovery of an austenitic Fe–13.50Mn–3.98Si–9.54Cr–4.51Ni alloy. Ce additions induce the formation of a large number of Ce‐rich particles, which act as austenitic grain refiners. Both grain refinement and the formation of Ce‐rich particles contribute to the strengthening of the matrix at 0.42 wt% Ce addition. In addition, Ce additions alter the MS temperature, which increases with Ce additions. Total shape recovery improves with 0.18 and 0.42 wt% Ce additions, but decreases with 0.96 wt% Ce addition. The beneficial effect of Ce addition in improving the shape recovery of the austenitic Fe–Mn–Si–Cr–Ni alloy is related to the enhancement of the elastic shape recovery component of the total shape recovery. However, the shape memory recovery due to the shape memory effect always decreases with the increase of the Ce content.

From the Truth Commission Report to the Stage and the Museum: The Artistic Dislocation of Violence from the Ecuadorian Chapter of 1983 to 2008

Are commemorative plaques enough to collectively and symbolically amend the horror of human rights violations? And if not, why is this strategy still the most common way for governments to pay their due respects to the families of the victims? This paper analyzes the suitability of the arts to address the reparation of the victims of human rights-related crimes perpetrated in Ecuador between 1983 and 2008, as investigated by the ad-hoc Truth Commission installed there afterwards. National and constitutional law, as well as international jurisprudence, will help to illuminate this study objective. Furthermore, in order to unpack the collective and individual dimensions of the right to memory of the victims, their families and their broader social fabric in which they are embedded, the notion of ‘memory sites’ is incorporated into the analysis. To contribute to the establishment of interconnections between the law and the arts, several initiatives for the recovery of memory in Ecuador are reviewed, from a critical stance. In this vein, the text foregrounds a centralizing proposal to consolidate an Ecuadorian aesthetics of memory, through institutionalization and museography, to address the governmental duty for reparations; but more importantly, to finally permeate the consciousness of an entire country whose past is still waiting to be incorporated into its present. By intertwining the legal, the artistic and the political layers present in this discussion, we advance in the direction of an aesthetics of memory that challenges the dominant narratives of genocidal violence.

Kapβ2 Inhibits Perioperative Neurocognitive Disorders in Rats with Mild Cognitive Impairment by Reversing the Nuclear-Cytoplasmic Mislocalization of hnRNPA2/B1.

Harmful stimuli trigger mutations lead to uncontrolled accumulation of hnRNPA2/B1 in the cytoplasm, exacerbating neuronal damage. Kapβ2 mediates the bidirectional transport of most substances between the cytoplasm and the nucleus. Kapβ2 guides hnRNPA2/B1 back into the nucleus and restores its function, alleviating related protein toxicity. Here, we aim to explore the involvement of Kapβ2 in neurodegeneration in rats with MCI following sevoflurane anesthesia and surgery. Firstly, novel object recognition test and Barnes maze were conducted to assess behavioral performances, and we found Kapβ2 positively regulated the recovery of memory and cognitive function. In vivo electrophysiological experiments revealed that the hippocampal theta rhythm energy distribution was disrupted, coherence was reduced, and long-term potentiation was attenuated in MCI rats. LTP was greatly improved with positive modulation of Kapβ2. Next, functional MRI and BOLD imaging will be employed to examine the AFLL and FC values of dynamic connectivity between the cortex and hippocampus of the brain. The findings show that regulating Kapβ2 in the hippocampus region enhances functional activity and connections between brain regions in MCI rats. WB results showed that increasing Kapβ2 expression improved the expression and recovery of cognitive-related proteins in the hippocampus of MCI rats. Finally, WB and immunofluorescence were used to examine the changes in hnRNPA2/B1 expression in the nucleus and cytoplasm after overexpression of Kapβ2, and it was found that nucleocytoplasmic mis location was alleviated. Overall, these data show that Kapβ2 reverses the nucleoplasmic misalignment of hnRNPA2/B1, which slows neurodegeneration towards dementia in MCI after sevoflurane anesthesia and surgery. Our findings may lead to new approaches for perioperative neuroprotection of MCI patients.

The Framework and Memristive Circuit Design for Multisensory Mutual Associative Memory Networks.

In this work, we propose a multisensory mutual associative memory networks framework and memristive circuit to mimic the ability of the biological brain to make associations of information received simultaneously. The circuit inspired by neural mechanisms of associative memory cells mainly consists of three modules: 1) the storage neurons module, which encodes external multimodal information into the firing rate of spikes; 2) the synapse module, which uses the nonvolatility memristor to achieve weight adjustment and associative learning; and 3) the retrieval neuron module, which feeds the retrieval signal output from each sensory pathway to other sensory pathways, so that achieve mutual association and retrieval between multiple modalities. Different from other one-to-one or many-to-one unidirectional associative memory work, this circuit achieves bidirectional association from multiple modalities to multiple modalities. In addition, we simulate the acquisition, extinction, recovery, transmission, and consolidation properties of associative memory. The circuit is applied to cross-modal association of image and audio recognition results, and episodic memory is simulated, where multiple images in a scene are intramodal associated. With power and area analysis, the circuit is validated as hardware-friendly. Further research to extend this work into large-scale associative memory networks, combined with visual-auditory-tactile-gustatory sensory sensors, is promising for application in intelligent robotic platforms to facilitate the development of neuromorphic systems and brain-like intelligence.

The Alzheimer multifactorial therapeutic agent E2F4DN prevents long‐term potentiation and cognitive loss and improves neuronal survival in homozygous 5xFAD mice

AbstractBackgroundDespite many decades of work, we still lack effective therapies against Alzheimer’s disease (AD), likely due to its complex etiology, which requires a multifactorial therapeutic approach. Among the variety of proposed etiological factors, synaptic failure has proof to be an early hallmarks of AD (Nat. Neurosci. 2016;19:443‐453), which precedes neuronal‐death (J. Neurosci. 2006;26:10129‐10140). We have recently shown, using both transgenic mice and gene therapy, that E2F4 is a multifactorial therapeutic target against AD (Neurotherapeutics 2021;18:2484‐2503, Mol. Neurobiol. 2022;59:3016‐3039), likely due to its homeostatic function (Int. J. Mol. Sci. 2022;23:12093). The phosphorylation of two conserved Thr residues of E2F4 is necessary to induce cognitive loss in AD (Neurotherapeutics 2021;18:2484‐2503). Therefore, we have developed a novel therapy consisting in neuronal expression of a dominant negative form of E2F4 (E2F4DN), which cannot be phosphorylated in the conserved Thr residues (Neurotherapeutics 2021;18:2484‐2503, Mol. Neurobiol. 2022;59:3016‐3039). In this work we wanted to verify whether the expression of E2F4DN could correct AD‐associated synaptic dysfunction and neuronal loss in 5xFAD mice, a known mouse model of AD.MethodWe expressed E2F4DN (intravenous administration of AAV‐E2F4DN) in 6 weeks old control and homozygous 5xFAD mice, and stereological, electrophysiological and behavioral studies were performed at 6 months. Cell counting was achieved by unbiased stereology in the CA1 hippocampal region. Cognition was analyzed through novel‐object location and contextual fear conditioning test. Synaptic function (basal synaptic transmission, presynaptic function and long‐term potentiation (LTP)) were measured by electrophysiological recordings of field excitatory postsynaptic potentials in hippocampal CA3‐CA1 synapses. Afterwards, the mechanism underlying memory recovery was evaluated analyzing protein levels of different subunits of the synaptic receptors AMPA and NMDA.ResultE2F4DN expression in neurons prevents neuronal death in the hippocampus (CA1) of homozygous 5xFAD mice, reaching similar values of cell number and CA1 volume to those observed in control mice. Moreover, E2F4DN prevents LTP loss leading to cognitive improvement, as 5xFAD mice treated with our therapy present a noticeable improvement hippocampal‐dependent memory. This effect correlates with the increase of key LTP regulators.ConclusionE2F4‐Based Gene Therapy improves neuronal survival, LTP and cognition, being a safe multifactorial approach for AD treatment.

The Correlation Between Anorexia Nervosa and Depression, Anxiety, and Memory

Neuropsychological functioning became an area of interest, researchers tended to gain more understanding and therapy options in anorexia nervosa (AN) based on the results of neuropsychology of anorexia nervosa. The study investigated the neuropsychological deficits in individuals with anorexia nervosa in comparison to healthy individuals. The main direction is to explore the effects of AN on the symptoms of depression, anxiety and memory performance in AN patient. Existing evidence on depression, anxiety and memory symptoms for outcomes related to AN was reviewed. The findings from current studies identify extensive evidence for the correlation between anorexia nervosa and depressive, anxious and memory symptoms. Anxiety and short-term memory demonstrated close relationships with AN. Most of the research found there was a positive correlation between the symptoms of depression and AN. The experiments about the recovery of memory in AN patient after treatment drew different conclusions. Some present controversies and areas in future studies are determined.

Memory Recovery Effect of a New Bioactive Innovative Combination in Rats with Experimental Dementia.

Alzheimer's disease manifests as a complex pathological condition, with neuroinflammation, oxidative stress and cholinergic dysfunction being a few of the many pathological changes. Due to the complexity of the disease, current therapeutic strategies aim at a multitargeted approach, often relying on a combination of substances with versatile and complementary effects. In the present study, a unique combination of α-lipoic acid, citicoline, extracts of leaves from olive tree and green tea, vitamin D3, selenium and an immune-supporting complex was tested in scopolamine-induced dementia in rats. Using behavioral and biochemical methods, we assessed the effects of the combination on learning and memory, and elucidated the mechanisms of these effects. Our results showed that, compared to its components, the experimental combination was most efficient in improving short- and long-term memory as assessed by the step-through method as well as spatial memory as assessed by T-maze and Barnes maze underlined by decreases in AChE activity (p < 0.05) and LPO (p < 0.001), increases in SOD activity in the cortex (p < 0.05) and increases in catalase (p < 0.05) and GPx (p < 0.01) activities and BDNF (p < 0.001) and pCREB (p < 0.05) levels in the hippocampus. No significant histopathological changes or blood parameter changes were detected, making the experimental combination an effective and safe candidate in a multitargeted treatment of AD.

Vitamin B12-folic acid supplementation improves memory by altering mitochondrial dynamics, dendritic arborization, and neurodegeneration in old and amnesic male mice

Memory impairment during aging and amnesia is attributed to compromised mitochondrial dynamics and mitophagy and other mitochondrial quality control mechanisms. Mitochondrial dynamics involves the continuous process of fission and fusion of mitochondria within a cell and is a fundamental mechanism for regulating mitochondrial quality and function. An extensive range of potential nutritional supplements has been shown to improve mitochondrial health, synaptic plasticity, and cognitive functions. Previous findings revealed that supplementation of vitamin B12-folic acid reduces locomotor deficits and mitochondrial abnormalities but enhances mitochondrial and neuronal health. The present study aims to explore the impact of combined vitamin B12-folic acid supplementation on mitochondrial dynamics, neuronal health, and memory decline in old age and scopolamine-induced amnesia, which remains elusive. The results demonstrated that supplementation led to a noteworthy increase in recognition and spatial memory and expression of memory-related protein BDNF in old and amnesic mice. Moreover, the decrease in the fragmented mitochondrial number was validated by the downregulation of mitochondrial fission p-Drp1 (S616) protein and the increase in elongated mitochondria by the upregulation of mitochondrial fusion Mfn2 protein. The increased spine density and dendritic arborization in old and amnesic mice upon supplementation were confirmed by the enhanced expression level of PSD95 and synaptophysin. Furthermore, supplementation reduced ROS production, inhibited Caspase-3 activation, mitigated neurodegeneration, and enhanced mitochondrial membrane potential, ATP production, Vdac1 expression, myelination, in old and amnesic mice. Collectively, our findings imply that combined supplementation of vitamin B12-folic acid improves mitochondrial dynamics and neuronal health, and leads to recovery of memory during old age and amnesia.

Mitochondrial protein prohibitin promotes learning memory recovery in mice following intracerebral hemorrhage via CAMKII/CRMP signaling pathway

Prohibitin (PHB) is a mitochondrial inner membrane protein with neuroprotective, antioxidant, and apoptosis-reducing effects. This study aimed to explore the role of PHB in pathological symptoms, behavioral deficits, and cognitive impairment in a collagenase-IV-induced intracerebral hemorrhage (ICH) murine model. In this study, mice that received collagenase IV injection were pretreated with PHB or saline 21 days prior to modeling. The role of PHB in memory and learning ability was monitored using the Morris water maze, Y-maze, and rotarod, social, startle, and nest-building tests. The effect of PHB on depression-like symptoms was examined using the forced swimming, tail suspension, and sucrose preference tests. Subsequently, mouse samples were analyzed using immunohistochemistry, western blotting, Perls staining, Nissl staining, and gene sequencing. Results showed that collagenase IV significantly induced behavioral deficits, brain edema, cognitive impairment, and depressive symptoms. PHB overexpression effectively alleviated memory, learning, and motor deficits in mice with ICH. PHB markedly inhibited the number of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling-positive cells and protein levels of ionized calcium-binding adapter molecule 1, glial fibrillary acidic protein, and interleukin-1β in the perihematomal region of ICH mice. PHB overexpression also remarkably promoted production of neurologin1 (NLGL1), and upregulated levels of Ca2+-calmodulin-dependent kinase II (CaMKII) and collapsin response mediator protein-1 (CRMP1) proteins. In conclusion, PHB overexpression can effectively alleviate the neurological deficits and neurodegeneration around the hematoma region. This may play a protective role by upregulating the expression of NLGL1 and promoting expression of CaMKII and CRMP1.