Ultrafiltration (UF) is a cost-effective technology for water reclamation, however, its use in tertiary filtration in cold climates is impacted by changes in secondary effluent characteristics and membrane properties under low temperatures. In the present study, a series of filtration tests with the effluents from sequencing batch reactors (SBR) were conducted to evaluate the impact of SBR operating temperature and UF filtration temperature on fouling of membranes used for tertiary treatment. The results demonstrated increased total membrane resistances of 27%, 45% and 82% over multiple filtration cycles when the SBR operating temperature was 20, 14 and 8 ℃, respectively. The greater total membrane resistance at low SBR operating temperatures was attributed to increased hydraulically reversible and irreversible resistances. Moreover, a reduction in filtration temperature induced 55% and 122% increases in total membrane resistance when compared to a filtration temperature of 20 ℃, with the effluents from the SBRs operated at 14 and 8 ℃, respectively. The greater total membrane resistance at low filtration temperature was attributed to increased intrinsic resistance and the greater retention of hydraulically reversible and irreversible foulants. Pearson correlation revealed that the fouling of membranes used for tertiary treatment was governed by both high and low molecular weight organics, which were generated to a greater extent at low SBR operating temperatures. In addition, the generation of biomass associated products (BAP) was greatly affected by SBR operating temperature resulting in higher fouling potential at lower temperatures. The outcomes of the present study provide insights into the effects of operating temperature on biological and filtration processes that influence the fouling behavior of membranes used for tertiary treatment and can be employed to assist with the design of fouling mitigation measures in cold climates.