Reaction Of Inulin Research Articles

Bioresponsive inulin‐azobenzene nanostructures for targeted drug delivery to colon

AbstractHere, a series of amphiphilic inulin‐azobenzene (IAb) conjugates has been synthesized by the reaction of inulin, a naturally occurring polysaccharide, with varying amounts of 6‐(3‐(4‐[4‐dimethylaminophenylazo]‐phenyl)‐thioureido)‐hexanoic acid (Azo‐ahx), an azobenzene linker. The resulting IAb conjugates (IAb‐1, IAb‐2 and IAb‐3 with 1, 3, and 5% substitution of the linker, respectively) self‐assembled into nanostructures in aqueous environment, which were then used to encapsulate the hydrophobic drug molecules, ornidazole (OZ) and 5‐fluorouracil (FU). Drug loaded and unloaded nanostructures were subjected to characterization by spectroscopic and dynamic light scattering (DLS) techniques. The sustained drug release behavior of these nanostructures was studied by dialysis bag method at different pH values. Enzyme‐responsive drug release behavior was investigated under the influence of inulinase. Besides, the best formulation, IAb‐1, not only showed antioxidant potential but also exhibited good wound healing properties as evidenced by in vitro scratch assay in HaCaT cells. The self‐assembled nanostructures of IAb‐1 were found be non‐toxic to cells even up to 700 μg/ml as tested by MTT assay on HEK 293 and HeLa cells. In conclusion, the potential of self‐assembled drug loaded inulin‐azo nanostructures has been demonstrated for the targeted drug delivery applications.

Synthesis of inulin and starch derivatives with a 2,6-diisobornyl-4-methylphenol (dibornolTM) fragment

UDC 577.151.4 Water-soluble polysaccharide derivatives containing covalently bound 2,6-diisobornyl-4-methylphenol (dibornol TM ) were synthesized by a Williams reaction of inulin and starch with 2,6-diisobornyl-4bromomethylphenol. The conversion of 2,6-diisobornyl-4-bromomethylphenol was 85–95%.

Inulin–iron complexes: A potential treatment of iron deficiency anaemia

The aim of this work was that to synthesize macromolecular derivatives based on inulin able to complex iron and useful in the treatment of iron deficiency anaemia. Carboxylated or thiolated/carboxylated inulin derivatives were obtained by single or double step reactions, respectively. The first one was obtained by reaction of inulin (INU) with succinic anhydride (SA) alone obtaining INU–SA derivative; the second one was obtained by the reaction of INU with succinic anhydride and subsequent reaction of INU–SA with cysteine; both derivatives were treated with ferric chloride in order to obtain the INU–SA–Fe III and INU–SA–Cys–Fe III complexes. Both complexes showed an excellent biodegradability in the presence of inulinase and pronounced mucoadhesion properties; in particular, thiolated derivative INU–SA–Cys showed greater mucoadhesive properties than polyacrylic acid chosen, as a positive reference polymer, and a good iron release profile in condition mimicking the intestinal tract. These results suggest the potential employment of such systems in the oral treatment of iron deficiency anaemia or as supplement of iron in foods.

Improved Synthesis and Physicochemical Properties of Alkoxylated Inulin

Inulin, a polydisperse reserve polysaccharide extracted from chicory, has been modified by alkoxylation in a water-free medium using a basic catalyst. The reaction of inulin with ethylene oxide, as well as propylene oxide, was performed in an organic solvent, N-methylpyrrolidinone, with triethylamine as basic catalyst in almost quantitative yields. The reaction resulted in a range of products with very specific properties such as highly increased water solubility, moderate surface activities and very high cloud points in electrolyte media.

Polymeric surfactants based on inulin, a polysaccharide extracted from chicory. 1. Synthesis and interfacial properties.

Inulin, the polydisperse reserve polysaccharide from chicory, has been modified by carbamoylation in organic solvents. The reaction of inulin with a range of alkyl isocyanates resulted, after crystalization, in a variety of carbamoylated inulins from which the interfacial properties were determined. The medium and long chain carbamoylated inulins showed a good to very good reduction of the interfacial tension which makes these biopolymers interesting in the field of biodegradable surface active agents.

In vitro biodegradation study of acetyl and methyl inulins by Bifidobacteria and inulinase

The purpose of this study was to investigate if acetylated and methylated inulins can be degraded by inulinase from Aspergillus niger and by Bifidobacteria, in order to determine their potential in colonic drug delivery. Methyl and acetyl inulins were synthesized by the reaction of inulin (Raftiline HP) with methyl sulfate and acetic anhydride, respectively. The degree of substitution (DS) and the structure of the reaction products were confirmed by 13C-NMR. Degradation by inulinase was investigated in a citrate buffer (0.05 M, pH 4) or with a mixture of citrate buffer and DMSO at 37°C. Biodegradation by Bifidobacteria was investigated under anaerobic conditions using an in-house prepared broth at 37°C for 2 days. The resulting products were analyzed chromatographically; the formation of short chain fatty acids was followed by measuring the pH of the incubation media. The results obtained suggest that acetylated and methylated inulins are not biodegradable, since no degradation product could be detected after incubation; a decrease in pH was clearly observed in control samples (pure fructose and inulin), but not in the derivatized inulin samples. The results can probably be explained by a change in conformation of inulin due to derivatization.

Synthesis of Carbamoylethyl Inulin and Carboxyethyl Inulin

Inulin etherified with carbamoylethyl groups and with carboxyethyl groups is described. Both materials were prepared by hydrolysis of the nitriles of cyanoethyl inulin, which was obtained from reaction of inulin with acrylonitrile.

A Modification of the Diphenylamine Method for Determination of Inulin

Colorimetric methods for the determination of inulin in blood serum or plasma and urine based on the reaction of inulin with diphenylamine have been described in recent years. The most widely used procedure is that of Alving, Rubin and Miller,1 in which the inulin-containing solutions are heated in closed tubes with diphenylamine and hydrochloric acid dissolved in ethyl alcohol. Tightly capped tubes are necessary because of the volatile solvent, and because the solution must be heated for 60 minutes at 100°C to obtain the maximum color. In this laboratory as well as in others, this method has been troublesome and the results not consistently accurate.For this reason a modification has been devised in which a less volatile solvent is used, thus permitting the use of open tubes. With this reagent a shorter period of heating is necessary for maximum color development and accurate results have been obtained in the determination of inulin in blood serum or heparinized plasma in concentrations as low as 4 mg pe...