Raw Sugar Beet Juice Research Articles

Investigating of artificial neural network potential to predict the properties of refined raw sugar beet juice by electrocoagulation process

Investigating of artificial neural network potential to predict the properties of refined raw sugar beet juice by electrocoagulation process

Modeling the efficiency of microfiltration process in reducing the hardness, improvement the non-sugar component rejection and purity of raw sugar beet juice

Modeling the efficiency of microfiltration process in reducing the hardness, improvement the non-sugar component rejection and purity of raw sugar beet juice

Bioethanol production from raw sugar beet cossettes in horizontal rotating tubular bioreactor.

Fossil fuels are still major energy sources, but the search for renewable energy sources has been encouraged. Bioethanol has been recognized as an alternative to fossil fuels and nowadays it represents more than 90% of the global biofuel production. Bioethanol production from raw sugar beet cossettes as a semi-solid substrate was studied. The study was carried out in the horizontal rotating tubular bioreactor (HRTB) with Saccharomyces cerevisiae as a microbial production strain. The impact of different combinations of HRTB operational parameters such as, rotation speed (5-15min-1), rotation type [constant or interval (3-15minh-1)] and working volume (ratio V W/V T=0.2-0.7) on the bioethanol production was examined. In this study, the highest bioprocess efficiency parameters ([Formula: see text]=0.47gg-1, E=87.36% and Pr=0.618gL-1h-1) were observed at 0.20 V W/V T, interval rotation of 12minh-1 and rotation speed of 15min-1. It has to be pointed out that bioethanol production efficiency in the HRTB was on the similar level as observed by bioethanol production from the raw sugar beet juice. Naturally present microorganisms of sugar beet could have a significant impact on bioethanol production. Higher yeast inoculation rate could reduce contaminant activities and, consequently, the bioethanol production efficiency would be improved.

Better Lime Purification of Raw Sugar Beet Juice by Advanced Fenton Oxidation Process

ABSTRACTThis work discusses the effects of Fenton oxidation pre-treatment on the lime purification of raw sugar beet juice using iron powder and hydrogen peroxide. During Fenton oxidation, particular attention was paid to the effect of reaction time and dosage of Fenton′s reagent to improve purification indexes of the raw juice throughout the clarification process. The total concentration of lime used for the purification was varied from 4.0 to 16.0 g of CaO/100 mL of juice. The results showed that higher color and total phenolic removal were achieved with an increase in H2O2 dosage and reaction time. At an initial pH of less than 6.2 and H2O2 concentration of 7000.0 ppm, color removal reached 85% and approximately 81% of total phenolic removal was achieved at a reaction time of 30 min (Treatment 5). It suggests that the quantity of CaO required for the efficient juice purification may be decreased from 16.0 g/100 mL for the control juice to approximately 12.0 g/100 mL for the juice obtained from Treatment 5. Fenton oxidation process improved the quality indexes of the purified juice, and can be combined with a conventional clarification process to achieve juice with high purity and low color.

Purification of Raw Sugar Beet Juice by Electrocoagulation

Purification of Raw Sugar Beet Juice by Electrocoagulation

Ozonation as an effective way to stabilize new kinds of fermentation media used in biotechnological production of liquid fuel additives.

BackgroundIntermediates from processing sugar beets are considered an attractive feedstock for ethanol fermentation due to their high fermentable sugar content. In particular, medium prepared from raw sugar beet juice seems to be suitable for use in fermentation processes, but it is microbiologically unstable and requires sterilization.ResultsThis study investigates the effect of ozone treatment on the activity of microbial cells from Bacillus subtilis,Leuconostoc mesenteroides, Geobacillus stearothermophilus, Candida vini, and Aspergillus brasiliensis in raw sugar beet juice. Raw sugar beet juice contaminated with 105 cfu/mL of the microbial strains was treated with gaseous ozone (ozone concentration in the oxygen stream 0.1 g O3/L O2, flow rate 6 L/h, 10–30 min, 18–20 °C). The number of microflora decreased to 0 cfu/mL after 30 min of ozone treatment in all studied samples.ConclusionsMedium prepared from raw sugar beet juice and sterilized by ozonation is suitable for use in fermentation processes.

Dead end ultra-filtration of sugar beet juice expressed from cold electrically pre-treated slices: Effect of membrane polymer on fouling mechanism and permeate quality

Abstract Purification of raw sugar beet juice expressed at ambient temperature (20 °C) from pretreated sugar beet slices by pulsed electric field (E = 600 V·cm− 1, tPEF = 10 ms, T = 20 °C) was studied on the laboratory scale by dead-end ultrafiltration tests. Polyethersulfone (PES) and Regenerated Cellulose (RC) membranes with the same nominal molecular weight cut-off of 30 kDa were used. Experiments were carried out in unstirred and stirred (at rotation speed of 500 rpm) mode at constant trans-membrane pressure of 2 bar. The effects of the membrane polymer (Polyethersulfone and Regenerate Cellulose) on the filtration flux and the permeate quality were studied. In order to identify the fouling mechanism, the filtration kinetics was modeled using combined fouling models. Results showed that the filtration productivity (filtration flux) and selectivity (rejection ratio of impurities) depended on the membrane polymer. The juice filterability was better with Regenerated Cellulose (RC) membrane while the polyethersulfone (PES) membrane ensured a better retention of impurities (colorants, proteins and colloids), leading to a higher juice purity. Experimental and models data adjustment showed that combined models were preferable to investigate the fouling mechanism for both unstirred and stirred filtration. The desirable sugar purity (95–96%) of filtrate implies the potential application of a novel process (PEF treatment-cold pressing-ultrafiltration) for sugar industrial production. Industrial relevance Pre-treatment by pulsed electric field PEF allowed cold or mild extraction of sucrose from sugar beet roots. The combination of PEF and ultra-filtration allowed high yield sucrose extraction and purification with less energy consumption. Membrane fouling analysis led to better filtration producibility. The obtained data can be useful for optimization of the sucrose production with high yield in industrial extraction processes.

Optimization of Fenton Oxidation Process for the Degradation of Color Precursors in Raw Sugar Beet Juice

The degradation of tyrosine and ferulic acid, via the Fenton process, was investigated in sucrose solution and raw sugar beet juice. Central composite design and response surface methodology were used to evaluate the relationships between degradation of the color precursors and the process parameters (i.e., initial concentration of the color precursors, solution pH, dosages of Fenton’s reagent, and reaction time). According to the statistical analysis, the optimized conditions for each variable in aqueous sucrose solutions including an initial concentration of the color precursors equal to 45.0 mg L−1 were as follows: reaction time: 30 min, pH 5.8, Fe2+ dosage: 1.34 mM, and H2O2 dosage: 53.6 mM. Under these conditions, 61.8 % of the color precursors and less than 2.5 % of sucrose were degraded after 30 min of reaction. The highest decolorization efficiency of the raw juice was obtained about 55 % with the addition of 1.34 mM Fe2+ and 53.6 mM H2O2 at an initial pH of 6.2. Using the same conditions, approximately 50 % of tyrosine was degraded in treated juice with Fenton’s reagent. Gel permeation chromatography analysis confirmed that the concentration of low molecular weight impurities in decolorized juice was considerably reduced. According to the results, Fenton oxidation process can be considered as a promising method for degradation of the color precursors and preparation of raw sugar beet juice with low color content.

Bentonite, temperature and pH effects on purification indexes of raw sugar beet juice to production of inverted liquid sugar

The present study deals with modeling of the effects of processing temperature (30–95°C), concentration of Bentonite (Bent) (1–5g/L) and pH (3.5–6) on color, turbidity, purity, ash and invert sugar content of purified raw sugar-beet juice. The used methodology was the face central composite design of response surface to optimize the key parameters (temperature of processing, concentration of Bent and pH) of the process. The main goal was to focus on the possibility to produce invert sugar directly from raw sugar-beet juice in conjunction with an alternative purification method and also comparison with the common conventional lime processing in the industry. In this way, second-order polynomial models were developed for dependent responses using least-square fit of regression analysis. The results of analysis of variance indicated that all three investigated independent parameters have significant influence on purity indexes of raw beet-juice. The optimum condition was a constant reaction time (30min) at temperature of 75°C, pH4.47 and Bent concentration of 1.7g/L which were determined on minimization of color, turbidity and ash content and maximization of invert sugar and purity. At this optimum point: color, turbidity, purity, ash and invert sugar content were found to be 1664 ICU420, 6.3 NTU, 93.9%, 0.55% and 1.6%, respectively. Also the clarified juice quality obtained by Bent method was improved greatly when compared with conventional limed–carbonated as the color and turbidity in clarified juice were removed by the rate of 35.55% and 76.09%, respectively.

Evaluation of ethanol fermentation parameters for bioethanol production from sugar beet pulp and juice

The aim of this study was to investigate the efficient utilization of sugar beet pulp, as well as raw, concentrated raw and thick sugar beet juice, for bioethanol production. Different fermentation conditions were examined. The influence of raw material pre-treatment (pasteurization or sterilization), type of batch culture process (stationary or shaken) as well as the type of Saccharomyces cerevisiae yeast preparation on the yields of the process were studied. Moreover, the fermentation process effectiveness was examined in connection with the quality of the obtained distillates. Sterilization, stationary batch culture and Safdistil C-70 yeast preparation were identified as the most profitable factors for sugar beet pulp fermentation, providing a high fermentation efficiency and ethanol yield (87.7% of theoretical ethanol yield). Concentrated raw beet juice resulted in a value of 94.2% of theoretical yield, and thick juice a 92.6% yield. The results suggest that bioethanol production from sugar beet pulp and sugar beet juice has promise as an alternative fuel. The raw spirits obtained from the sugar beet juice were characterized as having the lowest quantity of volatile by-products. Copyright © 2014 The Institute of Brewing & Distilling

Optimization of Raw Sugar Beet Juice Purification Process Conditions Using Bentonite by Response Surface Methodology

In this study, response surface methodology was used to determine the optimum raw sugar beet juice purification process conditions using bentonite to produce inverted liquid sugar. For this purpose, impact of factors on the purification process such as bentonite concentration (1-5 gr/li), pH (3.5-6) and temperature (35-95 ˚C) was investigated. For each response, a second-order polynomial model was developed using multiple linear regression analysis. Correlation coefficients of fitted regression models of color, turbidity, ash, adjusted purity and invert sugar for juice purification process were determined as 0.95, 0.89, 0.90, 0.91 and 0.96 respectively. Results showed that while increasing the bentonite concentration increased the turbidity content but the adjusted purity decreased, and had no significant effect on other parameters. At lower pH levels, separation of color and turbidity causes by bentonite, but was in creased it decreased separation of ash and also increased invert sugar levels. With increasing temperature from 35˚C, to 95˚C, Separation of color and turbidity causes and invert sugar and adjusted purity content as well as increased, while this variable has no significant effect on syrup ash content. The optimum conditions of raw sugar beet juice purification process using bentonite was determined to obtain minimum color, turbidity and ash with maximum invert sugar and adjusted purity which were verified experimentals were found to be bentonite concentration of 1.70 gr/li, pH of 4.47 and temperature of 75˚C. At this optimum point, color, turbidity, ash, adjusted purity and invert sugar content were found to be 1664 ICU420, 6.3 NTU, 0.55 %, 93.9 % and 1.60 %, respectively.

Economic purification of raw sugar in beet sugar factories

The refining of raw sugar in existing beet sugar factories after the regular campaign has become more popular to increase the white sugar output. Even the processing of a mixture of both raw cane sugar syrup and beet juice during the regular beet sugar campaign or afterwards with stored thick juice has already being practiced. Whereas the sugar house equipment can be used with almost no changes, the juice purification and the filtration need to be customized. The carbonatation principle is also applicable for the purification of the cane sugar syrup. The filtration can be performed in conventional two steps or as direct PKF (filter press) filtration eliminating the thickening filters. The paper presents different possibilities for improved and economical raw cane sugar syrup purification (carbonatation and filtration) by using already existing equipment in combination with new technology.

The effect of high electric field pulses on activity of sugar beet o-diphenol oxidase and yeast invertase

Activities of Saccharomyces cerevisiae invertase and sugar beet o-diphenol oxidase were relatively resistant to treatment with high electric field pulses (HEFP) irrespective of the conductivity, pH and concentration of their solutions and the number of electric pulses. The electric field strength ranged between 3 and 60 kV/cm and the number of pulses varied from 1 to 30. The yeast invertase was relatively resistant to HEFP treatment when the electric field strength ranged between 3 and 15 kV/cm since it lost only 10%–20% initial activity. When the electric field strength was increased to 17, 20 and 60 kV/cm, the activity of invertase was decreased by approximately 30%, 90% and 100%, respectively. Activity of o-diphenol oxidase contained in the raw sugar beet juice was not reduced by HEFP treatment.

The potential of microfiltration and ultrafiltration process in purification of raw sugar beet juice

In this paper, the effect of operation parameters of microfiltration (MF) and ultrafiltration (UF) processes on the performance of purification and de-colorization of raw juice have been studied. Experiments were carried out using the pilot plant MF/UF membrane system equipped to a tubular module with a ceramic membrane and spiral module with a polymeric membrane, respectively. The results showed that the clarified juice quality obtained from MF/UF method was improved greatly as compared to conventional limed–carbonated clarified juice in color, turbidity and hardness in the rate of 49.12%, 78.26% and 6.65%, respectively.

Sugar Beet Juice Fermentation byZymomonas mobilis Attached to Stainless Steel Wire Spheres

The fermentation of sugar beet juice as well as juice syrup medium by Zymomonas mobilis inoculum attached to stainless steel wire spheres was investigated. A semi-synthetic sucrose medium enriched with mineral salts and yeast extract was used as the control. It was established that raw sugar beet juice ensured good Zymomonas mobilis culture growth and slightly decreased ethanol synthesis applying both flame-burned and TiCl4-treated wire spheres as carriers (Qx = 0.05—0.06 g/l × h; Qeth = 1.02—1.22 g/l × h). High ethanol yield was also observed in juice medium (Y = 0.45-0.46 g/g), however, levan synthesis with this medium decreased. The application of juice syrup brought about less growth effect and ethanol synthesis as compared to juice medium. The use of semi-synthetic sucrose medium resulted in high levan production (Qlev = 0.6—0.7 g/l × h), however, reduced ethanol production by 40%. In conclusion, sugar beet juice or syrup is recommendable for the preparation of Zymomonas mobilis inoculum. The levan production stage has to be realized using an optimized semi-synthetic sucrose medium. The installed wire spheres filled with inocula provided the possibility for a repeated batch fermentation process, which could be recommended for both juice and semi-synthetic sucrose medium fermentation.

Scale up of fuel ethanol production from sugar beet juice using loofa sponge immobilized bioreactor

Production of fuel ethanol from sugar beet juice, using cells immobilized on loofa sponge was investigated. Based on ethanol productivity and ease of cell immobilization, a flocculating yeast strain, Saccharomyces cerevisiae IR2 was selected for ethanol production from sugar beet juice. It was found that raw sugar beet juice was an optimal substrate for ethanol production, requiring neither pH adjustment nor nitrogen source supplement. When compared with a 2 l bubble column bioreactor, mixing was not sufficient in an 8 l bioreactor containing a bed of sliced loofa sponges and consequently, the immobilized cells were not uniformly distributed within the bed. Most of the cells were immobilized in the lower part of the bed and this resulted in decreased ethanol productivity. By using an external loop bioreactor, constructing the fixed bed with cylindrical loofa sponges, dividing the bed into upper, middle and lower sections with approximately 1 cm spaces between them and circulating the broth through the loop during the immobilization, uniform cell distribution within the bed was achieved. Using this method, the system was scaled up to 50 l and when compared with the 2 l bubble column bioreactor, there were no significant differences ( P>0.05) in ethanol productivity and yield. By using external loop bioreactor to immobilize the cells uniformly on the loofa sponge beds, efficient large scale ethanol production systems can be constructed.