Subjective Ratings Research Articles

Fatigued or bored? Investigating the effect of different types of mental fatigue on 3 km running performance

Mental fatigue has been highly cited as having a negative impact on endurance performance. Few, however, have investigated whether different types of mental fatigue, namely active and passive fatigue, might affect endurance performance differently. This study used a repeated-measures design where 11 participants completed a 3 km run after three 32-min conditions: an actively fatiguing task (Task-load Dual-back; TloadDback); a passively fatiguing task (Mackworth Clock); and a control task (Documentary). Subjective ratings and performance on a second task (Flanker task) were taken before and after the 32-min tasks, while ratings of perceived effort, motivation and workload were taken during the 3 km run. Results showed that both fatigue conditions were mentally fatiguing, with the TloadDback rated as more demanding and the Mackworth Clock more boring and less motivating. Performance on subsequent tasks showed different effects: the TloadDback condition had the slowest responses on the post-test Flanker task, while the Mackworth Clock condition had the slowest completion time on the 3 km run, though this difference in completion times was non-significant. These results suggest that different cognitive tasks lead to different types of mental fatigue, which can lead to different outcomes on subsequent cognitive tasks but non-significant differences on subsequent physical tasks. This study highlights that to understand the influence of mental fatigue on physical performance, it is important to understand the cognitive tasks used and how different cognitive and physical tasks interact. Future work should examine whether performance outcomes in other areas commonly affected by mental fatigue, like technical and tactical sporting skills, are affected differently by active and passive fatigue.

The PLET (Portable Laparoscopic Endo-Trainer) study: a randomized controlled trial of home- versus hospital-based surgical training

PurposeThe purpose of this study was to assess the effect of training with a personal, portable laparoscopic endo-trainer (PLET) on residents’ laparoscopic skills.MethodsThe study took place at a tertiary-care academic university hospital in Switzerland. All participants were randomized to either a home- or hospital-based PLET training group, and surgical skill performance was assessed using five laparoscopic exercises. 24 surgical residents, 13 females and 11 males, were enrolled at any training stage. Nine residents completed the assessments. Endpoints consisted of subjective and objective assessment ratings as well as exercise time and qualitative data up to 12 weeks. The primary outcome was the difference in exercise time and secondary outcomes included performance scores as well as qualitative data.ResultsThe hospital-based training group performed exercises number 1, 3 and 4 faster at 12 weeks than at baseline (p = .003, < 0.001 and 0.024). Surgical skill performance was not statistically significantly different in any of the endpoints between the hospital- and home-based training groups at 12 weeks. Both the subjective and objective assessment ratings significantly improved in the hospital-based training group between baseline and 12 weeks (p = .006 and 0.003, respectively). There was no statistically significant improvement in exercise time as well as subjective and objective assessment ratings over time in the home-based training group. The qualitative data suggested that participants who were randomized to the hospital-based training group wished to have the PLET at home and vice versa. Several participants across groups lacked motivation because of their workload or time constraints, though most believed the COVID-19 pandemic had no influence on their motivation or the time they had for training.ConclusionThe PLET enhances laparoscopic surgical skills over time in a hospital-based training setting. In order to understand and optimize motivational factors, further research is needed.Trial registrationThis trial was retrospectively registered on clinicaltrials.gov (NCT06301230).

Collaborative imagination synchronizes representations of the future and fosters social connection in the present

From close friends to people on a first date, imagining a shared future appears fundamental to relationships. Yet, no previous research has conceptualized the act of imagination as a socially constructed process that affects how connected we feel to others. The present studies provide a framework for investigating imagination as a collaborative process in which individuals cocreate shared representations of hypothetical events-what we call collaborative imagination. Across two preregistered studies (N = 244), we provide evidence that collaborative imagination of a shared future fosters social connection in novel dyads-beyond imagining a shared future individually or shared experience in general. Subjective ratings and natural language processing of participants' imagined narratives illuminate the representational features of imagined events shaped by collaborative imagination. Together, the present findings have the potential to shift how we view the structure and function of imagination with implications for better understanding interpersonal relationships and collective cognition.

Defining Criteria for Disease Activity States in Systemic Juvenile Idiopathic Arthritis Based on the Systemic Juvenile Arthritis Disease Activity Score.

Our objective was to develop and validate cutoff values in the systemic Juvenile Arthritis Disease Activity Score 10 (sJADAS10) that distinguish the states of inactive disease (ID), minimal disease activity (MDA), moderate disease activity (MoDA), and high disease activity (HDA) in children with systemic juvenile idiopathic arthritis, based on subjective disease state assessment by the treating pediatric rheumatologist. The cutoff definition cohort was composed of 400 patients enrolled at 30 pediatric rheumatology centers in 11 countries. Using the subjective physician rating as an external criterion, six methods were applied to identify the cutoffs: mapping, calculation of percentiles of cumulative score distribution, the Youden index, 90% specificity, maximum agreement, and receiver operating characteristic curve analysis. Sixty percent of the patients were assigned to the definition cohort, and 40% were assigned to the validation cohort. Cutoff validation was conducted by assessing discriminative ability. The sJADAS10 cutoffs that separated ID from MDA, MDA from MoDA, and MoDA from HDA were ≤2.9, ≤10, and >20.6, respectively. The cutoffs discriminated strongly among different levels of pain, between patients with and without morning stiffness, and among patients whose parents judged their disease status as remission or persistent activity or flare or were satisfied or not satisfied with current illness outcome. The sJADAS cutoffs revealed good metrologic properties in both definition and validation cohorts and are therefore suitable for use in clinical trials and routine practice.

Measuring aviator workload using EEG: an individualized approach to workload manipulation.

Measuring an operator's physiological state and using that data to predict future performance decrements has been an ongoing goal in many areas of transportation. Regarding Army aviation, the realization of such an endeavor could lead to the development of an adaptive automation system which adapts to the needs of the operator. However, reaching this end state requires the use of experimental scenarios similar to real-life settings in order to induce the state of interest that are able to account for individual differences in experience, exposure, and perception to workload manipulations. In the present study, we used an individualized approach to manipulating workload in order to account for individual differences in response to workload manipulations, while still providing an operationally relevant flight experience. Eight Army aviators participated in the study, where they completed two visits to the laboratory. The first visit served the purpose of identifying individual workload thresholds, with the second visit resulting in flights with individualized workload manipulations. EEG data was collected throughout both flights, along with subjective ratings of workload and flight performance. Both EEG data and workload ratings suggested a high workload. Subjective ratings were higher during the high workload flight compared to the low workload flight (p < 0.001). Regarding EEG, frontal alpha (p = 0.04) and theta (p = 0.01) values were lower and a ratio of beta/(alpha+theta) (p = 0.02) were higher in the baseline flight scenario compared to the high workload scenario. Furthermore, the data were compared to that collected in previous studies which used a group-based approach to manipulating workload. The individualized method demonstrated higher effect sizes in both EEG and subjective ratings, suggesting the use of this method may provide a more reliable way of producing high workload in aviators.

Guided By AI: Navigating Trust, Bias, and Data Exploration in AI‐Guided Visual Analytics

AbstractThe increasing integration of artificial intelligence (AI) in visual analytics (VA) tools raises vital questions about the behavior of users, their trust, and the potential of induced biases when provided with guidance during data exploration. We present an experiment where participants engaged in a visual data exploration task while receiving intelligent suggestions supplemented with four different transparency levels. We also modulated the difficulty of the task (easy or hard) to simulate a more tedious scenario for the analyst. Our results indicate that participants were more inclined to accept suggestions when completing a more difficult task despite the ai's lower suggestion accuracy. Moreover, the levels of transparency tested in this study did not significantly affect suggestion usage or subjective trust ratings of the participants. Additionally, we observed that participants who utilized suggestions throughout the task explored a greater quantity and diversity of data points. We discuss these findings and the implications of this research for improving the design and effectiveness of ai‐guided va tools.

Null subject pronouns in Papiá Kristang (Malacca Creole Portuguese)

This paper investigates variation in the subject pronoun expression (SPE) in Papi&amp;aacute; Kristang (Malacca Creole Portuguese), a language without verb inflection. Previous accounts of Kristang (Baxter, 1988, 2012b; Hancock, 1969, 1973; Knowlton, 1964; R&amp;ecirc;go, 1942) present the language as predominantly displaying overt subject pronouns. However, the overall null subject pronoun rate in the oral corpus contemplated is substantial, registering a frequency of 45.7% (input pr. 0.32). As such, the paper focusses on the distribution of null subject pronouns and the constraints directing that distribution. The study considers the effects of priming, structural location, verb semantic class, and tense-mood-aspect (TMA), and addresses the effect of the individual speaker. It is found that while null subject pronoun use in Papi&amp;aacute; Kristang resembles, with minor differences, many attributes of this mechanism in other languages, it displays one notable trait: TMA particles favor null subject pronoun use. Extralinguistic constraints on SPE within the Papi&amp;aacute; Kristang speech community are seen to concern more the characteristics of individual speakers, and clusters of individuals, rather than predictors based on a pan-community age and gender classification.

The effect of exercise on gastrointestinal symptoms and body composition at different phases of the menstrual cycle

Women commonly experience bloating, stomach cramps, water retention, and weight gain at the beginning of their menstrual cycle, which cause fluctuations in body composition through extracellular changes, this may affect osmotic pressure, leading to gastrointestinal (GI) symptoms. The aim of this study was to investigate the effect of the menstrual cycle phase on extracellular changes and GI symptoms in females in response to exercise. Seven healthy premenopausal female recreational athletes (maximal O2 consumption: 50 ± 5.8 ml·kg-1) performed two running trials in an experimental crossover design during two distinct phases of the menstrual cycle, early follicular phase (EFP) and the mid-luteal phase (MLP). Each trial consisted of 120 min of running, 60 min at 110% lactate threshold and 60 min at 90%. Determination of cycle was calculated from calendar-based counting and urinary ovulation detection kits. The EFP was determined by self-reporting onset of menses. The day before each trial, participants followed a low FODMAP diet and completed a gastrointestinal symptom rating scale questionnaire. On the morning of the trial, a standard breakfast was consumed, upon arrival at the laboratory, body composition was recorded. Each hour, finger prick samples were taken to measure lactate and ensure performance did not exceed maximum, every 15 minutes, a visual analog scale (VAS) of subjective ratings between 0-10 cm of gut comfort was taken. Data was analysed using mean and standard deviation (subsequent statistical analysis will be conducted if an n = 10 is reached prior to the conference). During exercise, participants experienced a higher prevalence of GI issues throughout the EFP compared with the MLP. The most common complaints reported from the VAS were nausea; EFP 1 ± 1 cm, MLP 0 ± 0 cm. Flatulence; EFP 4 ± 3 cm, MLP 1 ± 1 cm, and stomach cramps; EFP 3 ± 2 cm, MLP 1 ± 1 cm. Total body weight (TBW) differences were noted between the EFP and MLP. TBW EFP pre-exercise was higher than MLP pre-exercise phase EFP pre-exercise: 38.7 ± 2.8 L compared to MLP pre-exercise at 35.8 ±5.5 L. Similar differences were demonstrated with extra cellular water pre-exercise EFP; 16.1 ± 1.1 L MLP; 15.2 ±1.0 L. During the EFP fluid retention is known to be higher than the MLP, and results are as expected. While the data suggests a seemingly minimal variation in Gl symptoms, individually, there appears to be a higher level of difference. More gastrointestinal symptoms were reported during exercise, which is consistent with the findings. There is a slight difference in GI symptoms between the EFP and MLP at this stage, indicating a slight shift in total body water.

Virtual reality assessment of a high-calorie food bias: Replication and food-specificity in healthy participants

BackgroundTheoretical models and behavioural studies indicate faster approach behaviour for high-calorie food (approach bias) among healthy participants. A previous study with Virtual Reality (VR) and online motion-capture quantified this approach bias towards food and non-food cues in a controlled VR environment with hand movements. The aim of this study was to test the specificity of a manual approach bias for high-calorie food in grasp movements compared to low-calorie food and neutral objects of different complexity, namely, simple balls and geometrically more complex office tools. MethodsIn a VR setting, healthy participants (N = 27) repeatedly grasped or pushed high-calorie food, low-calorie food, balls and office tools in randomized order with 30 item repetitions. All objects were rated for valence and arousal. ResultsHigh-calorie food was less attractive and more arousing in subjective ratings than low-calorie food and neutral objects. Movement onset was faster for high-calorie food in push-trials, but overall push responses were comparable. In contrast, responses to high-calorie food relative to low-calorie food and to control objects were faster in grasp trials for later stages of interaction (grasp and collect). Non-parametric tests confirmed an approach bias for high-calorie food. ConclusionA behavioural bias for food was specific to high-calorie food objects. The results confirm the presence of bottom-up advantages in motor-cognitive behaviour for high-calorie food in a non-clinical population. More systematic variations of object fidelity and in clinical populations are outstanding. The utility of VR in assessing approach behaviour is confirmed in this study by exploring manual interactions in a controlled environment.

Sex-based dyad differences on informant reports of participants’ daily functioning

Functional assessment in neurocognitive evaluation is often provided via informant reports. These subjective reports can vary based on the characteristics of informants and their relationships with participants, such as informant sex. However, whether informant sex intersects with participant sex to impact subjective ratings of participants’ daily functioning, and whether such effects mirror observed patterns in neuropsychological performance, has not been adequately examined with ethnoracially diverse samples. We examined differences among participant-informant sex-based dyads on subjective informant reports of participants’ daily functioning (assessed via the Functional Activities Questionnaire [FAQ]), and whether any observed differences on reported functioning corresponded to differences in objective participant performance on neuropsychological performance, among middle-aged and older Hispanic/Latino (n = 543), non-Hispanic Black (NHB; n = 1030), and non-Hispanic White (NHW; n = 5356) adults in the National Alzheimer’s Coordinating Center cohort (n = 6929). Analysis of covariance (ANCOVA) tests revealed significant dyad differences on FAQ scores in the NHB (p<.001) and NHW subsamples (p<.05), but not in the Hispanic/Latino subsample (p>.05). For the Hispanic/Latino and NHB subsamples, ANCOVA tests revealed no significant effects of dyad on neuropsychological performance (ps>.01), whereas for the NHW subsample, ANCOVA tests revealed significant dyad differences on performance in multiple cognitive domains (ps<.01). Nevertheless, the pattern of dyad differences on neuropsychological performance did not mirror the pattern of observed differences on FAQ scores in the NHW subsample. Findings and their implications, including potential contributions of other informant characteristics on observed dyad differences on reported functioning, are discussed.

Subjective Affective Responses to Natural Scenes Require Understanding, Not Spatial Frequency Bands.

It is debated whether emotional processing and response depend on semantic identification or are preferentially tied to specific information in natural scenes, such as global features or local details. The present study aimed to further examine the relationship between scene understanding and affective response while manipulating visual content. To this end, we presented affective and neutral natural scenes which were progressively band-filtered to contain global features (low spatial frequencies) or local details (high spatial frequencies) and assessed both affective response and scene understanding. We observed that, if scene content was correctly reported, subjective ratings of arousal and valence were modulated by the affective content of the scene, and this modulation was similar across spatial frequency bands. On the other hand, no affective modulation of subjective ratings was observed if picture content was not correctly reported. The present results indicate that subjective affective response requires content understanding, and it is not tied to a specific spatial frequency range.

Ingestible pills reveal gastric correlates of emotions.

Although it is generally held that gastrointestinal (GI) signals are related to emotions, direct evidence for such a link is currently lacking. One of the reasons why the internal milieu of the GI system is poorly investigated is because visceral organs are difficult to access and monitor. To directly measure the influence of endoluminal markers of GI activity on the emotional experience, we asked a group of healthy male participants to ingest a pill that measured pH, pressure, and temperature of their GI tract while they watched video clips that consistently induced disgust, fear, happiness, sadness, or a control neutral state. In addition to the objective physiological markers of GI activity, subjective ratings of perceived emotions and visceral (i.e. gastric, respiratory and cardiac) sensations were recorded, as well as changes in heart rate (HR), heart rate variability (HRV) and spontaneous eyes blinks as non-gastric behavioral and autonomic markers of the emotional experience. We found that when participants observed fearful and disgusting video clips, they reported to perceive not only cardiac and respiratory sensations but also gastric sensations, such as nausea. Moreover, we found that there was a clear relation between the physiology of the stomach and the perceived emotions. Specifically, when disgusting video clips were displayed, the more acidic the pH, the more participants reported feelings of disgust and fear; the less acidic the pH, the more they reported happiness. Complementing the results found in the deep gastric realm, we found that disgusting stimuli induced a significant increase in HRV compared to the neutral scenarios, and together with fearful video clips a decrease in HR. Our findings suggest that gastric signals contribute to unique emotional states and that ingestible pills may open new avenues for exploring the deep-body physiology of emotions.

Prospective Effects of Self-Rated Health on Dementia Risk in Two Twin Studies of Aging

Subjective health ratings are associated with dementia risk such that those who rate their health more poorly have increased risk for dementia. The genetic and environmental mechanisms underlying this association are unclear, as prior research cannot rule out whether the association is due to genetic confounds. The current study addresses this gap in two samples of twins, one from Sweden (N = 548) and one from Denmark (N = 4,373). Using genetically-informed, bivariate regression models, we assessed whether additive genetic effects explained the association between subjective health and dementia risk as indexed by a latent variable proxy measure. Age at intake, sex, education, depressive symptomatology, and follow-up time between subjective health and dementia risk assessments were included as covariates. Results indicate that genetic variance and other sources of confounding accounted for the majority of the effect of subjective health ratings on dementia risk. After adjusting for genetic confounding and other covariates, a small correlation was observed between subjective health and latent dementia risk in the Danish sample (rE = − .09, p < .05). The results provide further support for the genetic association between subjective health and dementia risk, and also suggest that subjective ratings of health measures may be useful for predicting dementia risk.

JOLT: A Noninvasive Solution for the Correction of the Jowl.

Sagging and loss of elasticity of the facial skin and tissues leads to jowling and an aged appearance. Jowling is a common reason for aesthetic surgical consultations. Prospective open-label study of the JOLT (jowl lifting) technique using hyaluronic acid (HA) fillers in healthy women 40-65 years of age with Fitzpatrick skin phototypes II-IV (n = 8). To be eligible, patients had jowling grade 2-3 on the jowl appearance scale as assessed by both the physician and patient. Treatment was administered at the baseline visit and consisted of an average of 6 mL of HA filler, 3 mL per side. Efficacy was assessed at baseline, week 4, and week 12. Assessment measures included the jawline rating scale (JRS) and a five-point global aesthetic improvement scale performed by two independent observers. Success was defined as an improvement of 1-2 grades on JRS. The mean JRS was improved from 2.75 at baseline to 1.53 at 90 days, which was considered a significant improvement. Mean global aesthetic improvement scale scores at 90 days were 1.44 ± 0.66 (range 1-3), indicating a "very much improved" appearance compared with baseline on both investigator and subject ratings. The procedure was well tolerated by all patients. Injection-related side effects (bruising and localized swelling) resolved spontaneously within 1 week of treatment. No other adverse events were reported. The JOLT technique effectively uses HA fillers to lift and tighten the lower face/neck and camouflage the remaining jowl, diminishing marionette lines, defining the mandibular border, and improving jowl sagging.

Research on the physiological and psychological impacts of extraordinary nature on emotions and restorative effects for young adults

Research indicates that exposure to natural environments positively impacts both physiological and psychological well-being. However, extraordinary, awesome landscapes, such as the mesmerizing Arctic and mysterious underwater scene, particularly contribute to enhancing emotional well-being. More exploration needs to be done exploration into the specific emotional and healing recovery effects of extraordinary natural landscapes. This study employs a combination of objective physiological measurements and subjective evaluations to investigate the therapeutic benefits of extraordinary natural landscapes. Sixty-two university volunteers experienced three ordinary natural landscapes and four extraordinary natural landscapes in a virtual reality setting. Participants wore wearable devices to collect physiological data, including EEG and HRV valves. Subjective questionnaires, semantic differential scales to assess the extraordinariness of landscapes, perceived restorativeness scale, and PAD emotional scale were utilized to evaluate recovery effects and emotional impacts. Results showed that: 1) Compared to ordinary nature, extraordinary nature demonstrates more significant recovery and emotional improvement effects. 2) The semantic differential scale evaluated the extraordinariness of natural landscapes across five dimensions: awe, remoteness, mystery, complexity, and uniqueness, with uniqueness being the predominant influencing factor. 3) As the extraordinariness of natural landscapes increases, the recovery effect also improves gradually. However, excessive exposure to extraordinary natural landscapes shows a diminishing therapeutic effect. These findings underscore the superior recovery effects of extraordinary nature, validated by physiological data and subjective ratings. The study highlights the potential of extraordinary nature as therapeutic material, offering a fresh perspective and quantitative data to study therapeutic landscapes.

EEG, EOG, Likert Scale, and Interview Approaches for Assessing Stressful Hazard Perception Scenarios

This study aimed to detect stressful hazard perception scenarios subjectively and objectively when using intelligent driving systems. We used electrooculography (EOG), electroencephalography (EEG), subjective ratings, and interviews to identify potential stressful hazard perceptions and record improvements in an intelligent navigation-guided pilot (NGP) system. Moreover, we analyzed electrophysiological data. Our study contributes to the use of engagement, concentration, and phase locking value connectivity based on EEG to support previous research methodologies using beta power, pupil size, fixation ratio, fixation duration, and subjective evaluations for investigating hazard perception. Our analyses showed that stressful hazard perception scenarios occurred mainly when encountering broken and solid lines, frequent lane changes, cars approaching suddenly, several cars driving in parallel, and the decision to change lanes but immediately pulling back upon using the NPG system. Our findings shed light on obtaining accurate results based on subjective and objective evaluations for developing intelligent driving systems.

Correlation between HOMA-IR value and urinary albumin excretion rate in nondiabetic young adult subjects

Correlation between HOMA-IR value and urinary albumin excretion rate in nondiabetic young adult subjects

Pupillometry as a Potential Objective Measurement of Pain Assessment in Healthy Volunteers.

Pain leads to activation of the autonomic nervous system and thus, among other things, to pupillary reflex dilation (PRD). Previous studies have already confirmed a correlation between the perception of pain and the pupillary reaction, measured using pupillometry. However, the previous study populations were under the influence of medication for analgesia in perioperative setting or suffered from pain. This study examines the relationship between pupillary reaction and pain perception in healthy controls and addresses the question of whether endogenous pain inhibition, clinically tested by conditioned pain modulation (CPM), can be quantified using pupillometry. Forty-two healthy volunteers (21 females, 21 males, mean age 27.9 ± 5.8 years, range 20-39 years) were included in this study. The PRD, as a measure of the pupillary reaction (variance from the base diameter in percent), was investigated during baseline, heat application and during CPM testing and results compared to the reported pain intensity on the numerical rating scale (NRS). The volunteers showed higher variances under painful conditions compared to the measurement at rest corresponding to higher sympathetic activity during pain. Volunteers with a higher variance, ie a stronger pupillary reaction, gave higher pain ratings than subjects with a lower pupil variance. However, there was no correlation between the NRS and PRD. PRD and pain ratings during CPM were significantly lower compared to heat pain application alone. However, there was no correlation between the calculated CPM effect and the PRD. Pupillometry is capable of objectively reflecting the pain response, eg pain relief through CPM testing. However, the CPM effect calculated from the subjective pain ratings and the objective PRD measurements is not associated suggesting that both measure different aspects of pain perception. It must be discussed whether the CPM effect can be the correct measure for the functionality of the pain system.

Use of Force by Male and Female Officers: Rates, Modalities, Effectiveness, and Injuries

The source article examined sex differences in: (1) use-of-force rates, (2) the use of different intervention options, (3) the effectiveness of different intervention options, and (4) subject and officer injury rates. Female officers were less prone to using force and prefer techniques requiring less physical strength (e.g., intermediate weapons), resulting in fewer injuries to suspects but a higher likelihood of sustaining injuries themselves. Police services may want to consider increased training in control techniques, like those used in jiujitsu, which are effective regardless of someone’s strength or build. There is a benefit associated with intermediate weapons, such as conducted energy weapons (CEWs), in achieving a balance between effectiveness and injury rates. Police services should equip officers with intermediate weapons and ensure officers receive proper training on their use.

Low-dose radiation as a preconditioning regimen for dendritic cell vaccines.

e14519 Background: Dendritic cells are potent antigen-presenting cells that induce the adaptive immune system to protect against cancer. While anti-tumor immunity can be improved with dendritic cell vaccines, it is not fully known what approved preconditioning agents, if any, improve dendritic cell vaccine-induced antitumor T-cell response and tumor elimination. We explored the effect of low-dose radiation (LDR), cyclophosphamide, and paclitaxel as preconditioning regiments for a dendritic cell vaccine. Methods: KP tumors were engineered with ovalbumin and orthotopically injected subcutaneously (SQ) into the flank of wild type B6 mice. Preconditioning regiments of a control, LDR (2 Gy), cyclophosphamide (20mg/kg), LDR (2 Gy) + cyclophosphamide (20mg/kg), and paclitaxel (20mg/kg) were given on day 4. Bone marrow dendritic cells (DCs) were electroporated with ovalbumin peptide SIINFEKL and then one million cells were injected i.v. on day 5 and 8. Blood collection was done and ovalbumin-specific T-cell populations were examined by tetramer staining for SIINFEKL-specific TCR over time. Tumor size was measured over time and survival data was analyzed accordingly. Results: LDR effectively produced a significant increase in CD8+ SIINFEKL tetramer+ T-cell count per uL of blood compared to the non-preconditioned group. In addition, this LDR preconditioning regiment produced a significantly improved tumor response over time. The overall survival rate of subjects receiving LDR was significantly improved compared to all other groups. Conclusions: Here, we find that LDR is most effective as a preconditioning regimen prior to dendritic cell vaccination for inducing an antitumor T-cell response and achieving tumor control over time. LDR provides a promising method to supplement dendritic cell vaccination and potentially improve patient outcomes. Further study is needed to confirm its efficacy.