Rate In Sheep Research Articles

Lupins and energy-yielding nutrients in ewes. II. Responses in ovulation rate in ewes to increased availability of glucose, acetate and amino acids.

An experiment, using a total of 320 Merino ewes, over two successive breeding seasons was conducted to investigate the separate effects of energy-yielding and protein-yielding nutrients on ovulation rate in sheep. The available energy-yielding or protein-yielding nutrients in the blood of sheep fed a maintenance pelleted ration were increased by either intravenous infusions of metabolites (acetate, glucose or acetate + glucose), feeding lupin grain, modification of the pattern of rumen fermentation to increase propionate production using the ionophore, lasalocid, or by feeding of ruminally undegradable protein (formaldehyde-treated casein). All treatments were given for 9 days prior to and including expected time of ovulation. Ovulation rates were measured by laparoscopy. In the first season (the 1984 experiment) when the effects of lupin grain, glucose + acetate and formaldehyde-treated casein were compared, ovulation rates were increased significantly in the group fed lupin (29%, P less than 0.001) and the group infused with glucose + acetate (24%, P less than 0.01). There was an apparent (17%) but statistically non-significant increase in the group fed casein. In comparisons between lupin grain, glucose, acetate, glucose + acetate, formaldehyde-treated casein and lasalocid in the 1985 experiment there were similar significant increases in ovulation rates in the groups fed lupin, and those infused with glucose or glucose + acetate (approximately 25%, P less than 0.001). The increase in the group infused with acetate was lower at 14% (P less than 0.05) and the increases of 7% in the groups fed casein or lasalocid were not significant.(ABSTRACT TRUNCATED AT 250 WORDS)

Wool growth and sulfur amino acid entry rate in sheep fed roughage based diets supplemented with bentonite and sulfur amino acids

In two experiments, sheep were offered a roughage-based diet supplemented with either cysteine or bentonite as a solid, or bentonite, cysteine or methionine added to their drinking water. Supplementation with cysteine as a solid had no effect on wool growth, while supplementation via drinking water had no effect on wool growth or cysteine entry rate into the blood. Supplementation with methionine via drinking water increased the entry rate of methionine into blood by 69% (P< 0.05) as measured by a continuous infusion of [35S]-methionine. This coincided with subsequent increases in wool growth of 16% (P< 0.05) compared to sheep fed a basal diet alone. Compared with the basal diet alone, supplementation with 30 g/day bentonite as a dry powder or 60 g/day as a suspension in drinking water increased wool growth by 19 and 20% respectively. Bentonite given as sole supplement did not increase the entry rate of either cysteine or methionine into the blood of sheep. When bentonite and sulfur amino acids were complexed or mixed, wool growth was not increased above that for bentonite or the amino acid alone.

Gastroduodenal motor activity associated with gastric emptying rate in sheep.

1. Gastric emptying rate was calculated from dye dilution and was measured as the net flow over periods of 5 days through an electromagnetic probe inserted into a T-cannula, 5 cm beyond the pylorus in conscious hay-fed sheep. The net aboral flow was related to the motor activity of the antrum and duodenal bulb which was recorded via chronically fixed strain-gauge transducers. Nichrome wire electrodes were also implanted in order to quantify the electrical activity of the musculature associated with the abomasal outflow in sheep sustained on a liquid diet infused at fixed rates. 2. The abomasal outflow occurred as gushes of 2.1-9.2 ml associated with antroduodenal contractions during 90-95% of the recording time. In the sheep eating 0.7 kg/day hay, the daily outflow of chyme was 5.1 l which increased to 7.5 l of less viscous chyme when the sheep was fed 1 kg/day lucerne hay. During gastric infusion of 3.9 and 7.9 l of the liquid diet, the outflow was 5.7 and 8.3 l/day respectively of low-viscosity chyme propelled as gushes of 5.9-6.9 ml during 95% of the recording time. 3. Increasing the viscosity of the liquid diet with guar-gum caused a reduction in both antroduodenal motility and flow. Dilution of the liquid diet with saline doubled the flow as a result of an increase in the amplitude of the antral contractions. Circadian variations in outflow were also related to concomitant variations in antral motility. 4. It is concluded that the minute-to-minute flow rate of chyme through the pylorus could be satisfactorily measured at the duodenal bulb level and related to the motor activity of the antrum and duodenum. The rate of abomasal outflow depended primarily upon the strength of antral contractions, while the viscosity of the chyme also appeared capable of exerting a major influence on the outflow rate.

Effects of Manipulating Rumen Fermentation and Outflow Rate in Sheep on Microbial Protein Yield as Estimated from Allantoin Excretion

New schemes describing the protein nutrition of ruminants consider both host and microbe requirements for nitrogenous compounds, truly absorbed amino N (TAAN) and rumen degradable N (RDN) respectively. This has made the prediction of the energetic efficiency of rumen microbial protein yield (E,MiN) crucial. However there is little guidance concerning the prediction of E,MiN since there are few consistent relationships in the literature. We face the dilemma that current techniques for measuring E,MiN are costly, invasive and laborious which makes it difficult to conduct large, designed and replicated experiments to investigate sources of variation. We have adopted the much simpler techniques of measuring urinary allantoin excretion to investigate sources of variation in E,MiN. Our objective was to investigate effects on E,MiN of varying rumen outflow rates by nutritional (level of intake and diet digestibility), and non-nutrition (supplementary NaHCO3) methods.

Plasma follicle stimulating hormone, ovulation rate and fertility in Merino ewes treated with bovine follicular fluid

Charcoal-treated bovine follicular fluid (bFF) given as four 5-ml subcutaneous injections to 13 Merino-Border Leicester ewes around the time of natural luteolysis suppressed ( P<0.01) plasma levels of follicle stimulating hormone (FSH) [from 1.08 ± 0.05 to 0.41 ± 0.03, mean ± s.e.m. of log e ( ng+ 1) / ml plasma]. This was followed ( P < 0.01) by hypersecretion or a rebound of FSH (to 1.46 ± 0.11) lasting 32 h in 10 of the treated ewes, and then by a further fall (to 0.73 ± 0.03, P < 0.05) before the surge (1.21 ± 0.07, P < 0.05) associated with the preovulatory surge of luteinizing hormone (LH). Plasma FSH at 56–72 h before the LH surge (i.e., at the time of the FSH rebound) was correlated with the subsequent ovulation rate ( n=13, r= + 0.73, P < 0.01). Fewer ewes treated with four injections of 2 or 5 ml of bFF than control ewes (injected with bovine plasma) became pregnant (28 of 41 vs. 38 of 41, χ 2 = 4.05, P < 0.05), although plasma progesterone was similar at Day 11 in treated and control ewes. It is concluded that plasma FSH during such a rebound influences the subsequent ovulation rate in sheep.

Plasma concentrations of FSH, LH and progesterone in sheep immunized against an androstenedione-protein conjugate.

A significant increase in the plasma concentrations of FSH (P less than 0.05) and LH (P less than 0.001) was observed during the luteal (Days 9-11) phase but not during the subsequent cloprostenol-induced follicular phase in androstenedione-immunized ewes compared to those in control ewes. Over the same time period the geometric mean (and 95% confidence limits) androstenedione antibody titres in the immunized ewes was 1/305 (1/158, 1/590) whereas they were not detectable in the controls. In the subsequent cycle, the ovulation rates were 1.6 +/- 0.2 for the immunized ewes and 1.1 +/- 0.1 for the control ewes (P less than 0.05) and the luteal progesterone concentrations were significantly higher in the immunized ewes compared to the controls (P less than 0.01). Collectively, these results suggest that active immunization against androstenedione leads to an increase in the plasma concentrations of both FSH and LH. The results are consistent with the hypothesis that FSH plays a central role in determining the ovulation rate in sheep.

Immunization against an inhibin subunit produced by recombinant DNA techniques results in increased ovulation rate in sheep.

Seven Merino-Border Leicester cross-bred ewes were immunized with a purified fusion protein, produced by recombinant DNA methods, of the alpha subunit of bovine inhibin. Four animals were immunized with the fusion protein alone and three with a conjugate made by coupling the fusion protein to keyhole limpet haemocyanin (KLH) using glutaraldehyde. Each animal received four injections of the fusion protein over 93 days. The animals were synchronized using progestagen sponges and subjected to laparoscopy for the determination of ovulation rates in two consecutive cycles (days 115 and 135). The immunized animals had overall mean ovulation rates for each cycle of 3.4 and 3.4 which was significantly (P less than 0.001) above the rates of 1.1 and 1.4 determined for the controls, which had either received no treatment (n = 5) or had been immunized with 300 micrograms KLH (n = 4). Analysis of antisera taken on day 115 showed significant fusion protein antibodies and iodinated inhibin-binding capacity in the test but not control groups. Furthermore, antisera to the fusion protein in four out of seven ewes neutralized the inhibin bioactivity of ovine follicular fluid in an in-vitro bioassay. These data demonstrate that neutralization of inhibin can be effected by immunization with bovine inhibin alpha subunit and that such immunization results in increased ovulation rates as predicted from the biological role of inhibin as a suppressor of FSH.

A note on the effects of health environment on heterosis for growth rate in pigs

Heterosis for growth rate in pigs is usually expressed, and reviews of European and North American studies have been given by Sellier (1976) and Johnson (1981). Kennedy and Moxley (1980) observed heterosis of 0·044 to 0·067 for weight at 154 days and attributed some of the crossbred advantage to heterosis for resistance to atrophic rhinitis. Similarly, McGuirk, Bourke and Manwaring (1978) attributed some of the heterosis for growth rate in sheep to heterosis for resistance to pneumonia. Although little attention has been paid to interactions between heterosis and health environment, Barlow (1981) concluded from an extensive review of studies involving farm and laboratory animals that heterosis is environment dependent and is enhanced by increased stress. Sellier (1976) commented that it would be useful to examine the degree of heterosis with ‘specific pathogen free’ pigs raised in a healthy environment, and this study examined heterosis for days to 90 kg in Yorkshire × Landrace pigs under three different environments with respect to health conditions.

Overfeeding during early pregnancy reduces peripheral progesterone concentration and pregnancy rate in sheep.

The 330 Merino ewes used in the study were placed with rams at a synchronized oestrus and, on Days 2-14 after mating, the ewes were placed in a feed lot and fed daily a low, medium or high ration (25%, 100% or 200% of maintenance respectively). Progesterone supplement was given to some ewes on Days 8-14 after mating by using a device containing 340 mg progesterone. Blood samples were taken from all ewes on Day 12 for measurement of plasma progesterone concentrations. On Day 14 after mating all ewes were returned to pasture. Pregnancy rate was determined by returns to oestrus and was later confirmed using ultrasound. There was a decline in the peripheral progesterone concentrations with increasing ration. The pregnancy rate in ewes fed a high ration was significantly reduced when compared with those of ewes fed a medium or low ration (48% vs 68 and 67% respectively; P less than 0.05). In ewes fed the high ration exogenous progesterone increased the pregnancy rate from 48 to 76% (P less than 0.01). Progesterone treatment did not influence pregnancy rates in ewes fed medium or low rations. The number of fetuses per ewe pregnant was not influenced by level of nutrition or progesterone treatment.

Ovarian features contributing to the variability of PMSG-induced ovulation rate in sheep.

In Exp. 1, ovulation rate was measured in three groups of Romanov ewes given two injections of 600 i.u. PMSG 3 weeks apart with the ewes intact (Group I, N = 8), a similar treatment with the ewes intact at the first injection and unilaterally ovariectomized at the second (Group II, N = 8), or unstimulated ewes which were hemispayed at the same time as Group II ewes (Group III, N = 6). In Exp. 2, the follicular population of one ovary was correlated with the number of ovulations induced by 600 i.u. PMSG in the contralateral ovary (10 Romanov ewes). From 8.4 +/- 1.8 (Group I) and 8.2 +/- 3.3 (Group II) CL at the first injection, PMSG-induced ovulation rate at the second injection decreased to 3.9 +/- 1.8 and 3.7 +/- 1.2 in Groups I and II respectively, a value similar for ewes with 1 or 2 ovaries. Furthermore, despite no major changes in the number of antral follicles after the first injection, there was no correlation (r = -0.09) between the response to the two successive injections in intact ewes. Comparison of the ovarian status of the ovary removed before the PMSG injection (Group II ewes of Exp. 1, ewes of Exp. 2) to the number of CL found in the remaining ovary demonstrated that PMSG-induced ovulation rate was not correlated with the overall antral follicle population (r = 0.62 in Exp. 1, r = 0.49 in Exp. 2).(ABSTRACT TRUNCATED AT 250 WORDS)

Growth Hormone-Releasing Factor Effects on Pituitary Function, Growth, and Lactation

Growth and lactation are complex processes controlled by several metabolic hormones such as insulin, glucagon, and thyroid hormones but most notably growth hormone. Growth hormone secretion is regulated by two hypothalamic hormones, somatostatin, an inhibitory regulatory factor, and growth hormone-releasing factor. The quantity and pattern of growth hormone secretion is ultimately regulated in concert by the secretion of both regulatory factors from the hypothalamus through the hypothalamo-hypophyseal portal system, where they exert their actions at unique pituitary receptors. Because the potential use of exogenous growth hormone administration for the stimulation of growth efficiency and lactation has been demonstrated, recent efforts have been directed toward the enhancement of production through manipulation of endogenous growth hormone secretion via its releasing factor. Thus far, releasing factor-stimulated growth and lactation has not been achieved to the same extent as that of exogenous growth hormone. Growth hormone-releasing factor has stimulated growth in two growth hormone-deficient children, as well as female, but not male, rats. Although all food-producing species tested to date respond to growth hormone-releasing factor with the appropriate growth hormone response, continuous or pulsatile administration of releasing factor has not resulted in increased growth rate in sheep, chicks, or hogs. Despite levels of circulating growth hormone that would be expected to produce a 30% increase in milk production if given exogenously to dairy cows, releasing factor-stimulated growth hormone secretion has resulted in only a 3 to 9% increase. It is clear from these studies that further developments are necessary to demonstrate the practical application of growth hormone-releasing factor.

Mitotic rate maturation in the Peyer's patches of fetal sheep and in the bursa of Fabricius of the chick embryo.

The metaphase arrest technique was used to quantitate the mitotic rate in sheep Peyer's patches (PP) and in the chicken bursa of Fabricius. It was shown that the mitotic rate in these tissues was the same whether or not the animal had been exposed to extrinsic antigen. The mitotic rate was calculated from the rate of accumulation of metaphase cells in tissue sections taken up to 4 h after i.v. vincristine. The follicular B cells in PP of fetal sheep at 3-5 days before birth, and in the bursas of chick embryos 2-3 days before hatching, none of which had experienced extrinsic antigen, entered mitosis at a rate of 5.2%/h and 7.5%/h, respectively. A similar analysis was done on animals at approximately 6 weeks of age, at which stage the PP and bursa had received chronic exposure to antigen from the gut. The mitotic rates were not significantly different from those in fetuses, i.e., 5.4%/h for PP and 6.0%/h for the bursa. In the follicles of both young lambs and juvenile chickens, the mitotic rate was 2-3 times greater in the outer, or cortical zone, than in the inner, or medullary zone. In a similar study of the thymus, no significant differences were found between the mitotic rates in fetal sheep and young lambs. The mitotic rate for the thymus was 15-20-fold less than for the PP. It is proposed that sheep PP, the chicken bursa and the thymus have in common the important characteristic of establishing a maximal rate of lymphocyte division prior to being exposed to antigen.

Increasing ovulation rate and lambing rate in sheep by treatment with a steroid enzyme inhibitor.

Treatment of ewes with a 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) inhibitor (Epostane) resulted in a significant increase in both ovulation rate and in the mean number of lambs per ewe lambing. The progestagen sponge plus 3 beta-HSD inhibitor treatment also caused a significant increase in oestrous cycle duration of approximately 1.5 days. Treatment of ewes with the 3 beta-HSD inhibitor caused a significant decrease in peripheral progesterone concentrations, which were reduced even further when 3 beta-HSD inhibitor treatment was given to ewes after insertion of a progestagen sponge. However, mean oestradiol concentrations were significantly higher in the two treatment groups, both at the end of the luteal phase and during the follicular phase of the oestrous cycle. These results demonstrate that ovulation rate and the production of lambs per ewe lambing can be significantly increased by 3 beta-HSD inhibitor treatment.

Measurement of volatile fatty acid production rates in sheep given roughage

Measurement of the irreversible loss rate (ILR) of acetate, propionate and butyrate by radioisotope dilution of the respective tracers in sheep offered chopped dried grass from continuous belt type feeders gave 'plateau samples', the specific radioactivity of which had unacceptably high variance (coefficient of variation [cv] of the five or six samples taken from any one animal over the last four hours of 10 hours infusion ranged from 16 to 57 per cent). In an experiment which attempted to identify the source of this variability the distribution of the liquid phase marker 51Cr EDTA into different areas of the rumen was examined during its administration either in liquid form or impregnated on paper. The paper provided better mixing but incurred considerable spillage problems which would be unacceptable where calculations of ILR were required. Radiographic observations were made to examine the mixing of radio-opaque liquid and solid phase markers in the rumen. A better mixing of both was achieved with intermittent than continuous feeding. When 1-14C- and 2-14C-labelled acetate were each infused into the rumen of four sheep given two levels of chopped dried grass by means of hourly feeders, samples of rumen liquor gave much lower variability of the mean plateau specific radioactivity values than was obtained in experiment 1 (cv less than 15 per cent in all but one sheep). The ILRs of acetate and the transfer quotients of carbon from acetate to butyrate obtained using 1-14C- and 2-14C-labelled acetate were similar.(ABSTRACT TRUNCATED AT 250 WORDS)

Ovulation rate in sheep selected for weaning weight or litter size.

Ovulation rate was measured by laparoscopy at two consecutive cycles on 366 ewes 2 yr old and over and 85 yearling ewes of five lines of Targhees from the base base population; 53 yearling linecross ewes were also included. The lines were two unselected controls (HCl and DC), two selected for 21 yr for increased 120-d weight (HW and DH) and one selected for 19 yr for multiple births (T). Ewes were synchronized in late July or early August at the start of the normal breeding season with intravaginal pessaries impregnated with 60 mg methylacetoxyprogesterone and examined at first and second estrus. Ovulation had occurred in both cycles in 327 (89%) and 177 (85%) of the mature and yearling ewes, respectively. Overall mean numbers of corpora lutea at first and second estrus were 1.42 and 1.63, respectively for ewes 2 yr and over and 1.20 and 1.44 for yearlings, indicating an effect of synchronizing treatment, season, flushing, or a combination of these. Among mature ewes, ovulation rate was higher (P less than .05) in DH (+.20), HW (+.19) and T (+.16) than in controls at first estrus, and in HW (+.29) and T (+.21) but not DH (-.04) at second estrus. Among yearlings, differences were significant only at second estrus (HW, +.40; T, +.35) and again not for DH (+.08). The failure of line DH to increase in ovulation rate from first to second estrus as did other lines was transmitted to linecross progeny. Body weight within line affected ovulation rate significantly, with a greater effect at second estrus, in both age groups. Adjustment for body weight removed the difference between HW and controls but not between T and controls. Repeatability of corpora lutea count was .27 and .25 for mature and yearling ewes, respectively.

Amniotic fluid volume and fetal swallowing rate in sheep.

To investigate amniotic fluid (AF) dynamics and volume regulatory mechanisms, we measured the concentration of radioiodinated (125I) serum albumin (RISA), 51Cr-labeled red blood cells (Cr-RBC), and 103Ru-labeled microspheres after injection into the amniotic cavity and determined AF volume and fetal swallowing rate in 22 singleton pregnant sheep. Under normal conditions 2-3 h were required for complete mixing of RISA and Cr-RBC within AF; however, when the fetus was dead only 3-5 h were required. AF volume of 17 sheep on the 5th postoperative day averaged 975 +/- 128 ml by RISA and 986 +/- 130 ml by Cr-RBC. AF volume determined with RISA and Cr-RBC correlated well. In contrast, AF volume measurement with microspheres produced erratic results. The disappearance rate of the labels in 17 ewes on the 5th postoperative day averaged 4.9 +/- 0.7%/h for RISA and 5.5 +/- 0.7 for Cr-RBC, and the calculated rates of fetal swallowing were 935 +/- 78 ml/day by RISA and 1,085 +/- 102 by Cr-RBC. In dead fetuses the disappearance rates were almost zero, suggesting that the labels disappear mainly by swallowing. Absolute volume swallowed and swallowed volume per fetal weight correlated with gestational age. AF volume correlated with fetal weight. Radiolabeled albumin or red blood cells may be used to simultaneously measure amniotic fluid volume and the rate of fetal swallowing. Furthermore it appears that fetal swallowing increases with gestational age.

Rumen fermentation pattern, postruminal protein flow and wool growth rate of sheep on a high-barley diet

Variations in the wool growth rate of sheep fed diets containing cereal grains were investigated. In experiment 1, eight sheep consuming similar quantities of a pelleted diet of barley grain and lucerne chaff (60:40 by weight) were selected for high (n = 4) or low (n = 4) wool growth rate. These wool growth differences were eliminated by feeding a high-protein, predominantly roughage diet. Subsequent reintroduction of the high-grain diet regenerated wide between-sheep variance, but there was little evidence of repeatability of performance on this ration. These variations in wool growth were examined in experiment 2 in terms of rumen fermentation pattern and postruminal protein flow. Ten sheep with simple cannulae in the rumen and abomasum were given a pelleted lucerne chaff diet for 14 weeks and the high-grain diet of experiment 1 for a further 16 weeks. The variance of all measured characters was low on the lucerne diet, but the high-barley diet generated wide variance in rumen fermentation pattern, protein flow from the rumen and wool growth rate. Fermentations characterized by high ammonia level, high minimum fluid pH and high butyrate molar proportion were associated with high urinary nitrogen output, high diet digestibility and low postruminal protein flow. Wool growth rate was closely related to protein flow on the high-grain diet alone (r2 = 0.77, P &lt; 0.01) and for the high-grain and lucerne diets considered together (r2= 0.86, P &lt; 0.001).

Fecal pH and Defecation Rates of Eight Ruminants Fed Known Diets

The effects of five rations and four dates postcollection on fecal pH and defecation rates were determined for eight ruminant species in penned enclosures. Only axis deer (Axis axis) had a uniquely different fecal pH (P < 0.05). Species differences and date of analysis resulted in 37.9 and 25.0%, respectively, of the observed variation in fecal pH, whereas ration accounted for only 15.4%. Fecal pH decreased as time postcollection increased. Fecal pH was of limited utility in identifying specific origin of fecal pellets. Whitetailed deer (Odocoileus virginianus) had a higher defecation rate (X = 19.6 groups/day, SE = 2.3) (P < 0.05) than did any other species. Our data suggest that previous estimates of defecation rate for white-tailed deer may be low. J. WILDL. MANAGE. 48(3):807-813 The fecal pellet group count is a common method of assessing population trends and habitat use for wild ruminants. However, problems arise when a range is occupied simultaneously by two or more ruminants because of the inability to distinguish between fecal pellets of different species. Several techniques for testing specific origin of fecal pellets have been proposed, including size/shape comparisons (Sanders 1963, Ogren 1964), fecal pH (Howard 1967, Nagy and Gilbert 1968, Howard and DeLorenzo 1974, Krausman et al. 1974, Hansen 1978, Beasom et al. 1982), scale patterns of ingested hairs (Beasom et al. 1981), and electrophoresis (Scribner et al. 1984). Differentiation by fecal pH has been the most popular approach to date, but consistency of results has been sporadic (see Beasom et al. 1982). Indeed, the inability to separate pH variation because of diet similarities from those due to inherent physiological differences has confounded most attempts (Peek and Keay 1979, MacCracken 1980, Beasom et al. 1982). MacCracken (1980) and Beasom et al. (1982) concluded that fecal pH was primarily a function of diet. Thus, if the species of interest exhibit appreciable dietary overlap, fecal pH probably will not be an accurate method of species identification (Hansen 1978, MacCracken 1980, Beasom et al. 1982). The inability to identify the specific origin of fecal pellets is especially troublesome on the Edwards Plateau of Texas, where rangelands commonly are grazed by white-tailed deer, sheep, goats, and several species of exotic ungulates. While documenting habitat use by white-tailed deer following brush removal (Rollins 1983), we encountered as many as eight sympatric ruminant species. In addition to sheep, goats, and white-tailed deer, exotic axis, fallow (Cervus dama) and sika deer (C. nippon), aoudads (Ammotragus lervia), and blackbuck antelope (Antelope cervicapra) were present. Defecation rates are another aspect of pellet group methodology that have been contested in recent years. Accurate estimates of defecation rates are necessary for 'Manuscript T-9-336, Coll. of Agric. Sci., Texas Tech Univ. 2 Present address: Department of Agronomy, Oklahoma State University, Stillwater, OK 74078. 3Present address: Department of Entomology, Texas A&M University, College Station, TX 778432475. J. Wildl. Manage. 48(3):1984 807 This content downloaded from 207.46.13.124 on Wed, 22 Jun 2016 06:00:46 UTC All use subject to http://about.jstor.org/terms 808 FECAL PH AND DEFECATION RATES * Rollins et al. computing deer-days use or other population indices. Early reports suggested that the defecation rates of white-tailed and mule deer (0. hemionus) were about 13 times/day (McCain 1948, Eberhardt and Van Etten 1956). Smith (1964) suggested a slightly higher rate (13-14 times/day), whereas Rogers et al. (1958) reported a mean rate of 15 times/day. Collins and Urness (1981) reported a rate of 21-23 times/day for mule deer. Factors that may affect defecation rate were summarized by Neff (1968) and include forage intake (Rogers et al. 1958), forage moisture content (Longhurst 1954), abrupt changes in diet and age of animal (Smith 1964), and, possibly, stress (Neff 1964). Collins and Urness (1981) and Irby (1981) found that animal activity stimulated defecation. Few reports are available on the defecation rates for ruminants other than white-tailed or mule deer. Ogren (1964) reported a defecation rate of 12.7 times/ day for aoudads. Bailey and Putman (1981) reported that fallow deer defecated about 10 times/day, whereas Dinerstein and Dublin (1982) found a much higher defecation rate in captive axis deer (9 = 28 times/day). Longhurst (1954) found that defecation rate in sheep varied from 13-16 times/day depending on forage moisture content. We are not aware of any published estimates of defecation rates for Spanish or Angora goats, sika deer, or blackbuck antelope. We initiated a penned study in April 1982 to determine the effects of different diets on fecal pH values and defecation rates of eight ruminants. We wish to thank C. S. Schreiner, IV, for use of animals and facilities, D. Frantzen for providing assistance during the feeding trial, and D. B. Wester for assistance with statistical analyses. R. L. Preston, R. Pritchard, and R. J. Warren reviewed the manuscript.

Spectral analysis of fetal heart rate in sheep: The occurrence of respiratory sinus arrhythmia

Respiratory sinus arrhythmia is a pattern of rhythmic variation in the heart rate that occurs at the frequency of respiration and is mediated principally by the vagus nerve. Spectral analysis can decompose the variance of a series of sequential measures into constituent frequencies to measure and verify whether there is respiratory sinus arrhythmia in utero in the fetal lamb. Recordings of heart period were obtained from electrodes implanted under fetal skin in six chronic preparations. Respiratory rate and heart period were recorded immediately after delivery and daily for the next 5 days. Respiratory sinus arrhythmia was clearly demonstrated in the neonatal lambs, and the same frequency of respiratory sinus arrhythmia was observed in the fetus and in the newborn lamb (0.8 to 0.1 Hz). There was a reproducible pattern of change in respiratory sinus arrhythmia from 27 days before delivery until term, with a decline in the amplitude of respiratory sinus arrhythmia 4 to 8 days before delivery. We conclude that respiratory sinus arrhythmia was demonstrated in fetal sheep and may serve as an indicator of the integrity of the central nervous system in the fetus and the neonate.

Effects of pasture type on the growth and wool production of weaner sheep during summer and autumn

The productivity of weaner sheep grazing lucerne, white clover, red clover or Persian clover and pastures sown with subterranean clover and perennial ryegrass, cocksfoot or tall fescue, was measured in summer-autumn trials incorporating four soil types in each of three years. During summer and autumn, the mean growth rate of sheep grazing lucerne, white clover or Persian clover pastures was 50 g/d compared with 1 g/d for sheep grazing perennial grass-subterranean clover pastures. Sheep grazing strawberry clover on reclaimed swampland, and sheep in a feedlot (fed hay and lupin grain) averaged 100 and 75 g/d, respectively. Sheep grazing red clover averaged 45 g/d over years 1 and 2. In year 2, a subterranean clover-dominant pasture was included in the study. Its feeding value was similar to that of the perennial grass-subterranean clover pastures. Sheep grazing legume pastures, and sheep in the feedlot, consistently produced more wool (up to 700 g/head) than sheep grazing the perennial grass-subterranean clover pastures. The concentration of neutral detergent fibre in herbage from legume pastures was frequently lower (P&lt; 0.05) and the concentration of crude protein was frequently higher (P&lt; 0.05) than in herbage from grass pastures. The dry matter digestibility of legume herbage was higher (P&lt;0. 05) than that of grass herbage on only one occasion. Measurements of crude protein and neutral detergent fibre of pasture in December accounted for 67% of the variation in liveweight gain (P&lt;0.01). Ewe weaners grazing red clover pasture exhibited an increase (P&lt; 0.05) in teat length of 3.9 mm in 21d of grazing. Oestrous activity of ewe weaners was related to liveweight gain (r = 0.77). The implications of the results for agricultural systems are discussed.