Event Abstract Back to Event PDGF-A activated signaling pathways and molecular mediators in glioma growth and migration Juhi Singh1*, Kedarlal Sharma1 and Prakash P. Pillai1 1 The Maharaja Sayajirao University of Baroda, Division of Neurobiology, Department of Zoology, Faculty of Science, India Gliomas contribute to 50% of the brain tumors characterised by its highly invasiveness, aggressive tumorigenicity and resistance to chemotherapy and radiation. The molecular mechanisms governing these characteristics involve numerous physiological and genetic alterations. One of the common genetic alteration or defect observed in gliomas is the presence of PDGF (Platelet Derived Growth Factor) autocrine loop marked by the co-expression of the PDGF and its receptor. PDGFR amplification in glioblastoma has been frequently linked to its abnormal proliferation and metastasis. Stimulation of PDGFR by its ligand results in the activation of several signalling pathways such as ERK1/2, PI3K and ROCK pathways causing aberrant growth, proliferation and migration. However, the role of PDGF mediated intracellular signalling in glioma growth and migration has not been much explored. The objective of the present study focuses on the intracellular pathways involved in PDGF receptor inhibition mediated suppression of Rat C6 glioma growth and migration. To study the effect of PDGFR inhibition on MAPK-ERK and ROCK signalling, pERK and pMLC levels were determined in C6 cells exposed to PDGFR inhibitor or PDGF-A. We found reduction in pERK and pMLC levels in PDGFR inhibitor group compared to control and PDGF-A treated group and it suggested MAPK-ERK and ROCK signalling act downstream of PDGFR. Further, growth and migration of C6 cells were studied and found to be reduced in PDGFR inhibitor, MEK inhibitor and ROCK inhibitor compared to control and PDGF-A treated groups. Also, PDGFR inhibition lled to up-regulation of BDNF (Brain Derived Neurotrophic factor), MeCP2 (Methyl CpG binding Protein 2) and GFAP (Glial Fibrillary Acidic Protein) expressions which were found to be associated in regulation of glioma progression. Thus, the study suggests PDGFR inhibition regulates the growth and migration by modulating ERK-ROCK signalling which can be a future potential target for the treatment for glioma. Keywords: Migration, therapy, molecular genetics, Signalling pathway, Glioma growth Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: Poster Presentation Session Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Singh J, Sharma K and Pillai P (2016). PDGF-A activated signaling pathways and molecular mediators in glioma growth and migration. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00198 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Ms. Juhi Singh, The Maharaja Sayajirao University of Baroda, Division of Neurobiology, Department of Zoology, Faculty of Science, Vadodara, Gujarat, India, juhivsingh@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Juhi Singh Kedarlal Sharma Prakash P. Pillai Google Juhi Singh Kedarlal Sharma Prakash P. Pillai Google Scholar Juhi Singh Kedarlal Sharma Prakash P. Pillai PubMed Juhi Singh Kedarlal Sharma Prakash P. Pillai Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.