Rapid Determination Of Histamine Research Articles

Rapid determination of histamine in cheese by employing fluorescence enhancement of quantum dots-molecularly imprinted polymer

A fluorescent surface imprinted polymer was prepared by grafting Cadmium Telluride (CdTe) quantum dots into the methacrylic acid polymerization on the surface of silica. And a rapid turn-on fluorescence biosensor based on fluorescence enhancement effect was developed for the rapid and sensitive detection of histamine, which only took 8 min with a sensitive detection limit of 14.3 μmol/L. The cheese samples were tested by employing the fluorescent imprinted polymer, and the recovery was obtained in the range of 92.1–102%. The proposed histamine biosensing method shows great advantages, including no need for derivatization and reduced consumption of organic reagents. This has an important guiding role in the rapid detection of biological amines and other fluorescent responsive substances in complex systems.

Tryptophan capped gold–silver bimetallic nanoclusters-based turn-off fluorescence sensor for the determination of histamine

Tryptophan capped gold-silver bimetallic nanoclusters (Trp-Au/Ag BNCs) based turn-off fluorescence sensor has been developed for the simple and rapid determination of histamine. Sensor parameters such as the effect of solvent, irradiation time etc. were optimized and further studies were done using these results. Fluorescence quenching of BNCs happens with the incremental addition of histamine. This might be due to the aggregation of BNCs induced by the electrostatic force of attraction between the negatively charged BNCs and amino functional groups of histamine. The sensor showed good sensitivity for the determination of histamine with a linear range of 8.5 × 10−5 M to 4.0 × 10−6 M and a limit of detection (LOD) of 9.0 × 10−7 M. Practical utility of the proposed sensor has been proved through spike recovery analysis in synthetic physiological fluids.

Rapid determination of histamine and tyramine content in milk by azo dye-spectroscopy

Rapid determination of histamine and tyramine content in milk by azo dye-spectroscopy

Determination of Histamine in Wine Samples by Flow-Injection Analysis and Multivariate Calibration

A flow injection method for the rapid determination of histamine in wines is proposed. This method is based on the reaction of the analyte with 1,2-naphthoquinone-4-sulfonate to form a derivative detected by UV-Vis absorption spectroscopy. The amine derivatization is carried out at pH 9.2 in a PTFE coil heated at 60°C. Experimental conditions are not able to provide selectivity for histamine with respect to other amino compounds so that its response is interfered with by other amines and amino acids present in the wine matrix. Here, multivariate calibration methods have been used to overcome the lack of selectivity. As a result, wines can be directly analyzed without removing interferences. For this purpose, spectra taken at the peak maximum have been used as multivariate data to be further analyzed with partial least square regression. The number of significant latent variables to be used in the construction of the calibration models as well as a preliminary evaluation of the prediction performance have been estimated by leave-one-out cross validation. The quantification of histamine concentrations in wine samples is satisfactory with an overall prediction error about 5%.

A rapid determination of histamine and 3-methylhistamine in human plasma by high performance liquid chromatography

Histamine plays an important pathophysiological role in allergy, inflammation, gastric acid secretion, microcirculation and neurotransmission. 3-Methylhistamine is a prominent metabolite of histamine. Different methods for determination of histamine in biological fluids have been developed. In the present study, a simple, simultaneous determination of histamine and 3-methylhistamine by HPLC (precolumn derivatization with fluorescamine) was developed in human plasma using fluorescence detection with 1-methylhistamine as the internal standard. Linear regression analysis of the ratios of the concentrations of histamine and 3-methylhistamine (X) against peak height ratios (Y) yielded the following: y = 0.0073x − 0.0096 (R 2 = 0.990) and y = 0.0077x − 0.0111 (R 2 = 0.989). In conclusion, it was possible to detect histamine and 3-methylhistamine below 5 ng mL−1 in 1 mL plasma.

水産物中のヒスタミン簡易測定法の開発

水産物中のヒスタミン簡易測定法の開発

Rapid determination of histamine and its metabolites in mice hair by ultra-performance liquid chromatography with time-of-flight mass spectrometry

The rapid determination of histamine (HA) and several metabolites, i.e., 1-methylhistamine (MHA), imidazole-4-acetic acid (IAA), and 1-methyl-4-imidazole-acetic acid (MIAA), in mice hairs was performed by ultra-performance liquid chromatography with time-of-flight mass spectrometry (UPLC-TOF-MS). HA and MHA, having a primary amino group ( NH 2) in their structures, were first labeled with 4-( N, N-dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F) at 60 °C for 45 min in the mixture of 0.1 M borax (pH 9.3) and acetonitrile (CH 3CN). On the other hand, 4-( N, N-dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ) was used for the labeling of a carboxylic acid group ( COOH) in IAA and MIAA in the presence of 2,2′-dipyridyl disulfide (DPDS) and triphenylphosphine (TPP). The reaction with DBD-PZ was completed at 50 °C after 2 h. The resulting derivatives of HA and the metabolites were perfectly separated using an ACQUITY UPLC™ BEH C 18 column (1.7 μm, 100 × 2.1 mm, i.d.) with the mixture of 20 mM HCOONH 4 and CH 3CN (8:2). The structures of HA and the metabolites were identified from the protonated-molecular ions [M + H] + and the de-protonated-molecular ions [M − H] − of authentic compounds, obtained from TOF-MS measurement. A good linearity was achieved from the calibration curves, obtained by plotting the peak area ratios of the analytes relative to the internal standard (IS), i.e., histamine-α,α,β,β- d 4 (HA- d 4) or 4-imidazolecarboxylic acid (ICA), against the injected amounts of each compound (1.0–25 pmol, r 2 = 0.998). The detection limits of HA and the metabolites were less than 1 pmol. The proposed method was applied to the determination in the hair shafts of C3H mice. The average concentrations of HA, MHA, IAA and MIAA in 1 mg of the hair shafts were 16.3 pmol ( n = 7), 21.6 pmol ( n = 3), 6.6 pmol ( n = 3) and 7.1 pmol ( n = 3), respectively. Because the proposed method provides good mass accuracy and the trace detection of HA and several metabolites in hair, this analytical technique seems to be applicable for the determination of various biological compounds in hair.

Simple and rapid determination of histamine in food using a new histamine dehydrogenase from Rhizobium sp.

A colorimetric enzyme assay for the quantitative analysis of histamine in food has been developed using a new histamine dehydrogenase (HDH) from Rhizobium sp. The HDH specifically catalyzes the oxidation of histamine but not other biogenic amines such as putrescine and cadaverine. The principle of our photometric assay is as follows. The HDH catalyzes the oxidative deamination of histamine in the presence of 1-methoxy PMS (electron carrier), which converts WST-8 (tetrazolium salt) to a formazan. This product is measured in the visible range at 460 nm. The correlation between the histamine level and absorbance was acceptable, ranging from 0 to 96 μM with histamine standard solutions, corresponding to 0 to 30 μM of the reaction solution ( r = 1.000, CV = 1.0% or less). Assays of canned tuna (in oil and soup) and raw tuna with 45–675 μmol/kg histamine added showed good recoveries of 96–113, 98–108, and 100–106%. The histamine contents of a commercial canned tuna and fish meal containing histamine at high concentrations were determined using the new method and other reference methods (HPLC method, Association of Official Analytical Chemists official method, and two commercial enzyme immunoassay test kits). This simple and rapid enzymatic method is as reliable as the conventional methods.

Fluorimetric Determination of Histamine in Fish Using Micellar Media and Fluorescamine as Labelling Reagent

An analytical method based on the use of fluorescamine to produce a fluorescent derivative with histamine and combined with micellar-enhanced fluorescence detection of the formed complex is developed for the sensitive and rapid determination of histamine in fishes. The fluorescence properties of the obtained complex in water and micellar solutions of sodium dodecyl sulfate (SDS), cetyltrimethylammonium chloride (CTAC) and brij-700 are reported. Physicochemical variables influencing the sensitivity of the method (pH, micellar, fluorescamine and NaCl relative concentrations) have been optimized. The stability of the formed complex, as shown by kinetic study, depends on the pH of the solution. Linear calibration curves allowing an effective histamine determination were established with large linear dynamic range (LDR), and low limits of detection (LOD) between 0.5 and 33 ng mL-1, according to the solvent. Application to the analysis of fish samples (sardines) yielded satisfactory results. The method seems to be suitable for environmental fish quality control.

Improved enzymatic method for the rapid determination of histamine in wine

Fermented foods are frequently contaminated by histamine generated by microorganisms possessing histidine decarboxylase activity. The ingestion of large amounts of histamine can cause serious toxicological problems in man. Thus, it becomes important to set a reliable method for rapid histamine quantification in foods. The detection of bacteria exhibiting histidine decarboxylase activity is also important to estimate the risk of contamination of food. Previous enzymatic methods used to quantify histamine in fish gave erroneously high values due to interference when applied to wine. A new enzymatic method is described that allows the direct determination of histamine concentrations in this type of sample. It can be used for the detection of histamine in synthetic media, grape must or wine (white, rosé, red) without polyphenols or sugar interferences. This new enzymatic method shows a good correlation (R 2 = 0.996, p<0.001) between the histamine concentrations and absorbances in the interval 0.4–160 mg l−1. Comparison between this enzymatic method and a high-performance liquid chromatography method showed a high correlation (R 2 = 0.9987, p<0.001). A miniaturized enzymatic method is also proposed, which is particularly useful when high numbers of samples must be analysed.

Immunoassay method for detection of histamine in foods

A novel screening immunoassay for histamine was used for detection of histamine in different foodstuffs. The detection limit of this assay was 20 µg kg-1. The concentration of histamine varied between 182-982 µg kg-1 in sauerkraut, cheese and fish samples and 26-18433 µg l-1 in milk, sparkling wine and wines. The applied competitive enzyme immunoassay (ELISA) seemed a reliable technique for simple and rapid determination of histamine in food.

Selective determination of histamine by flow injection analysis

A flow injection analysis (FIA) method for the determination of histamine is described. Control of reaction timing allows exploitation of a transient, chemical-kinetic increase in selectivity that occurs when o-phthalaldehyde reacts with histamine. The molar fluorescence ratio (selectivity) of histamine/histidine reaches a maximum value of 800 in 32 s, precluding the need for separation of histamine from histidine, spermidine, and other potential interferences in biological samples. On-line dilution prevents matrix effects and affords a linear response up to approximately 4.45 mM histamine, or 500 mg of histamine free base/100 g. Under these conditions the detection limit (3 times peak-to-peak baseline noise) is 5.5 pg (corresponding to 0.60 mg of histamine free base/100 g of sample) and throughput is 60 injections per hour. The high sensitivity and high selectivity of the method allow the rapid determination of histamine in fish with minimal sample conditioning and will find application in the determination of endogenous histamine as well, such as in blood plasma and brain tissue.