The study of the impact of urbanization on river ecosystems is an important part of constructing sustainable cities. How to quantitatively study the impact of urbanization on river ecosystems is the difficulty of urban ecological research. This study quantitatively investigated the effects of LULC on water quality and diatom assemblages in urban streams by correlation analysis, multivariate analysis, and path analysis. The results showed that (1) the percentage of LULC type in buffer 600m of reference stream sites is significantly different from that of urban stream sites. In reference sites, the average percentage of green was 94.2%, barren 3.3%, and impervious surface 2.5%. In urban upstream sites, the average percentage of impervious surface was 63.1%, green 32.8%, water 3.3%, and barren 0.8%. In urban downstream, the average percentage of impervious surface was 59.0%, green 36.5%, water 2.7%, and barren 1.8%. (2) One-way analysis results showed that water quality variables were significantly differences among the sites. The correlation analysis results indicated that LULC had a significant influence on water quality. Green had a significant negative correlation with high concentrations of NO3-N, NH4-N, and Cond. but positively correlated with MSUBST. RDA results showed that the selected water quality variables, MSUBST, and LULC types have a significant impact on the spatial patterns of the diatom assemblages. (3) Path analysis results showed that both LULC types and water quality variables exerted significant effects on diatom assemblages. This study first clarifies the quantitative relationships among LULC types, water quality, and diatom assemblages in the Beijing area. And green land was positively correlated with water quality and river ecosystems. We believed that increasing green space in urban core areas is an effective measure for improving water quality and restoring river ecosystems in the urban area.
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