Quantitative Determination Of Carbamazepine Research Articles

Application of TLC-smartphone method for the analysis of carbamazepine in plasma

A new, simple, rapid, low-cost, and robust thin layer chromatography-smartphone method has been developed for the qualitative and quantitative determination of carbamazepine in human plasma. Liquid–liquid extraction (LLE) with dichloromethane was used for sample purification. The drug of interest was separated on TLC aluminum coated with silica gel; and a mixture of cyclohexane, chloroform, and acetic acid (4:4:2 v/v/v) was used as the mobile phase. The sample spot was visualized under a UV chamber at 254 nm then photographed by a smartphone. The intensity of each spot was quantified by videodensitometry. The method was validated according to the guideline of the ICH Q2 (R1). The results showed good linearity (R > 0.99), high sensitivity, and reliable robustness. For accuracy and precision, acceptable relative standard deviations (RSD) were reached (<15%). The limits of detection and quantification were 0.94 µg/mL and 2.8 µg/mL, respectively. The comparison to HPLC method didn’t show a significative difference (p = .314). The analysis of carbamazepine in samples charged with several interferences showed good recovery (>90%), except for phenobarbital (recovery = 72%). It was concluded that the proposed TLC-smartphone is a viable method that can be used for the analysis of carbamazepine in human plasma.

DEVELOPMENT AND VALIDATION OF A METHOD USING INSTRUMENTAL PLANAR CHROMATOGRAPHY FOR QUANTITATIVE ANALYSIS OF CARBAMAZEPINE IN SALIVA

An instrumental planar chromatography method for quantitative analysis of carbamazepine in saliva was developed. The linearity, precision, detection and quantification limits, accuracy and selectivity of the method were validated. The method was linear between 0.50 and 15.00 ng/spot, with a regression coefficient of 0.999. The intra-assay variation (repeatability) was between 5.1% and 7.4%, and the inter-assay (reproducibility) was between 5.6% and 7.4%. The detection limit was 0.18 ng, and the quantification limit was 0.54 ng. The method proved accurate, with a recovery percentage of 109.8%, and it was selective for the active principle tested. In conclusion, is a good method for the quantitative determination of carbamazepine in saliva

Liquid Chromatographic Determination of Carbamazepine in Tablets

A simple and rapid liquid chromatographic method is described for the qualitative and quantitative determination of carbamazepine in tablet composites and individual tablets, using the internal standard technique. Analyses were performed on a C-18 reverse-phase column with tetrahydrofuran-methanol-water (8 + 37 + 55) as the mobile phase. A linear relationship was obtained between detector responses at 254 nm and amounts of carbamazepine injected ranging from 0.2 to 1.7 micrograms. The coefficient of variation for 10 consecutive injections of a standard preparation was 0.4%. Recoveries of carbamazepine from 100 and 200 mg tablets averaged 101.4 and 99.7%, respectively. Assay results for commercial tablets analyzed by the proposed method agreed favorably with those obtained by the method of USP XXI. The assay results for individual tablets indicated that deviations from the average value and the range of individual values are much wider with the compendial method than with the proposed method.

Quantitative determination of carbamazepine and carbamazepine-10,11-epoxide in rat brain areas by multiple ion detection mass fragmentography

A specific and sensitive method is described for the simultaneous determination of carbamazepineand carbamazepine-10,11-epoxide in biological specimens by combined gas chromatography-mass spectrometry. Cytenamide is used as the internal standard for quantitation. Kinetics of the distribution of the drug and its metabolite in plasma and in different areas of rat brain are reported. Determinations are possible at the nanogram level.

Quantitative determination of carbamazepine and carbamazepine-10,11-expoxide in rat brain areas by multiple ion detection mass fragmentography

A specific and sensitive method is described for the simultaneous determination of carbamazepineand carbamazepine-10,11-epoxide in biological specimens by combined gas chromatography-mass spectrometry. Cytenamide is used as the internal standard for quantitation. Kinetics of the distribution of the drug and its metabolite in plasma and in different areas of rat brain are reported. Determinations are possible at the nanogram level.