Method For Quantitative Analysis Research Articles

Pengaruh Kepemimpinan terhadap Peningkatan Kinerja dan Motivasi Pegawai Bapedda Kabupaten Penukal Abab Lematang Ilir

Mulya Happenny. This study aims to get an in-depth picture of the effect on improving the performance and motivation of Bappeda employees of Penukal Abab Lematang Ilir (PALI) Regency. The place that became the object of research was the Bappeda of Penukal Abab Lematang Ilir Regency which was located on Merdeka Street, Handayani Mulya Village, Talang Ubi District. The background of the problem in this study is the decline in employee performance from 2019 to 2021. Since 2020 in PALI Regency there are many echelon 2 positions. The Head of Division at Bappeda also holds the position of Head of Service in another place. So the researchers tried to examine whether there is an effect on improving the performance and motivation of employees under these conditions. The method used is a quantitative analysis method using the SPSS v 25 application. Data collection uses a Likert questionnaire. The number of samples taken was 50 employees using the saturated sample method or using the total population. Data analysis used is validity and reliability test, classical assumption test, simple linear regression analysis, and multiple linear regression as well as t-test, F test, and coefficient of determination test (Adjusted R Square). The results of the study indicate that leadership has a varied effect on increasing employee motivation and performance. Leadership in the Infrastructure Sector on improving performance with the magnitude of the influence of 25.67%, 74.5% from the Head of the Ecososbud Sector, and 26.6% from the Head of the Program. The difference in the magnitude of the effect is also shown in the employee motivation variable, namely 27.6% from the Head of the Infrastructure Division, 25.6% from the Division Head, and 30% from the Program Head. The simultaneous influence shows that leadership will affect performance improvement by 90% and 33.4% effect on employee motivation. From the results of the study, it can be suggested to the leadership to improve the quality of good leadership and make improvements in terms of coordination and communication with subordinates.

Research on the Relationship between Urban and Rural Educational Resources Allocation and Educational Equity

This comprehensive study delves deeply into the relationship between the allocation of educational resources in urban and rural areas and educational equity. It takes the unbalanced distribution of educational resources between urban and rural regions as its starting point and employs quantitative analysis and field research methods to explore this complex relationship. The findings reveal that the uneven allocation of educational resources has a significant negative impact on educational equity, resulting in a substantial disparity between urban and rural students in terms of access to high-quality education. Policy attention deviation, an imperfect management mechanism, and insufficient investment in education are identified as the key reasons for this unbalanced allocation. Consequently, this study recommends that policymakers increase investment in rural education, reform the management mechanism, and optimize resource allocation to promote the realization of educational equity. The results of this research hold great significance for understanding the unequal distribution of urban and rural educational resources and providing valuable references for policymaking.

Scientific Article Writing Training for Teachers of State Elementary School 2 Liligundi

The purpose of this training activity is to improve the understanding and skills of teachers at SDN 2 Liligundi in writing scientific articles. The number of participants in this training is 10 people. This training activity will be carried out using a face-to-face method. The data collection method used to measure the success of this training is the training assignment method and questionnaire. While the data analysis method used is the quantitative descriptive data analysis method. The indicator of the success of this training is the increasing ability of teachers at SDN 2 Liligundi in compiling scientific articles, especially scientific articles from research they have conducted at school. The results of this training are to improve the understanding and skills of teachers at SDN 2 Liligundi in writing scientific articles, especially scientific articles sourced from Classroom Action Research that has been carried out by teachers. It can be concluded that the scientific article writing training activity for teachers at SDN 2 Liligundi has gone very well and is beneficial for improving teacher professionalism in compiling scientific articles.

Program for forming psychological readiness for professional communication in future pre-school education teachers during the process of studying at university

Introduction. The article deals with the problem of preparing specialists of the preschool education system for future professional activity in the process of studying at higher school. The authors present the basic concept for the creation and implementation of the main stages of the program for the formation of psychological readiness among students of preschool education for professional communication with all subjects of the educational process. The modular principle of designing a program based on the main components of the structure of psychological readiness is discussed, and the need to increase the practical training of future teachers of preschool education is noted. Materials and Methods. The research used: theoretical methods: analysis of scientific publications, psychological and pedagogical literature; synthesis and interpretation, method of generalization and systematization of information, modeling of the process of formation of psychological readiness for professional communication; empirical research methods: psychological and pedagogical experiment, testing, direct and indirect observation, proximetric techniques; data analysis methods: methods of mathematical statistics, quantitative and qualitative analysis of the results. Results. The authors substantiate and concretize the scientific and theoretical foundations, approaches and principles, organizational, psychodidactic and psychological-pedagogical conditions, present a detailed approbation of the program, assess the dynamics of the formation of psychological readiness for professional communication among future teachers of preschool education. The program allows undergraduate students to consistently master general cultural and general psychological communicative competencies; skills, abilities, actions of planning, designing, building, accumulating individual experience of interpersonal and professional communication with different subjects of the educational process; building personalized communication models with different target groups and subjects of education; to form the initial style of professional communication of the future teacher, his value-semantic, a substantial and structural-organizationaloutline. Discussion and Conclusions. The performed research has shown the need and possibility of systematic and systematic formation of psychological readiness for professional communication among students of preschool education in the process of studying at a university. The psychological readiness of students of preschool education has its own characteristics and correlates with the requirements for the professional competencies of a future teacher of preschool education.

UBIQUITY OF WORKPLACE DIVERSITY AND INCLUSION FOR EMPLOYEE WELL-BEING

Building a sense of belonging in the workplace is essential for both organizational efficacy and employee well-being in a world that is progressively more interconnected and globalized. Through the perspectives of diversity, equality, and inclusion (DEI), this study explores the various facets entailed in developing such a culture. The study examines the effects of DEI initiatives on employee engagement, satisfaction, and productivity using both qualitative and quantitative research methods of analysis. Through extensive surveys and in-depth interviews with professionals from a range of industries, this study pinpoints critical tactics and industry best practices that businesses can use to improve DEI. The results demonstrate how important varied representation, fair policies, and inclusive leadership are in fostering a feeling of community among staff members. Additionally, the study examines the difficulties and impediments that organizations have when promoting DEI and provides workable methods to get past them. KEYWORDS: Diversity, Equality, Inclusion, Wellbeing.

An integrated quantitative method of risk analysis and decision making for safety manipulation of a forest firefighting aircraft by using physical models of multi-hazard factors

Although firefighting aircrafts are effective for fighting forest fires, they are affected by a variety of risks related to forest fires during flight operations such as thermal radiation, temperature rise, decrease in air density, and changes in updraft aerodynamics. Quantifying these influences poses a significant challenge. Our research reveals that safety standards predominantly hinge on technical indicators like flight height, speed, and angle of attack, which makes it possible to quantify the above effects. A general method framework for analysing firefighting flight safety criteria was constructed in this study. Using relevant physical models, such as heat radiation, temperature, air density, and updraft in a forest fire environment, safety criteria with an analytical form were established. The corresponding safety constraints of each criterion were calculated quantitatively, and the most stringent constraint was determined based on multiple factors to obtain a comprehensive safety constraint boundary composed of the critical height, stall speed, and critical angle of attack. Finally, the effectiveness of the method is validated through a simulation case. The research results show that the constrained boundary calculation method proposed in this study can provide a scientific foundation for developing technical specifications for forest aviation safety and aviation fire task plan.

A method for quantitative spatial analysis of immunolabeled fibers at regenerative electrode interfaces

BackgroundRegenerative electrodes are being explored as robust peripheral nerve interfaces for neuro-prosthetic control and sensory feedback. Current designs differ in electrode number, spatial arrangement, and porosity which impacts the regeneration, activation, and spatial distribution of fibers at the device interface. Knowledge of sensory and motor fiber distributions are important in optimizing selective fiber activation and recording. New MethodWe use confocal microscopy and immunofluorescence methods to conduct spatial analysis of immunolabeled fibers across whole nerve cross sections. ResultsThis protocol was implemented to characterize motor fiber distribution within 3 macro-sieve electrode regenerated (MSE), 3 silicone-conduit regenerated, and 3 unmanipulated control rodent sciatic nerves. Total motor fiber counts were 1485 [SD: +/- 50.11], 1899 [SD: +/- 359], and 5732 [SD: +/- 1410] for control, MSE, and conduit nerves respectively. MSE motor fiber distributions exhibited evidence of deviation from complete spatial randomness and evidence of dispersion and clustering tendencies at varying scales. Notably, MSE motor fibers exhibited clustering within the central portion of the cross section, whereas conduit regenerated motor fibers exhibited clustering along the periphery. Comparison with Existing MethodsPrior exploration of fiber distributions at regenerative interfaces was limited to either quadrant-based density analysis of randomly sampled subregions or qualitative description. This method extends existing sample preparation and microscopy techniques to quantitatively assess immunolabeled fiber distributions within whole nerve cross-sections. ConclusionsThis approach is an effective way to examine the spatial organization of fiber subsets at regenerative electrode interfaces, enabling robust assessment of fiber distributions relative to electrode arrangement.

Content Analysis of the Effects of Domestic Violence on Child Victims in the Movie Precious

Domestic violence is a dynamic phenomenon that can occur in all walks of life. Given the role of film as a tool to convey ideas and influence the audience, research on the effects of domestic violence in the movie Precious is interesting to do. The rampant news about domestic violence makes this research relevant because this movie can provide an overview of the destructive impact of domestic violence on children as victims. The aim of this research is to find out what the physical, psychological, sexual, and social effects are due to domestic violence experienced by the main character in the film Precious. This research uses a quantitative content analysis method with a descriptive approach. Data will be measured using a measurement table. The research population is the movie Precious with a duration of 1 hour 50 minutes, with a sample of scenes containing the effects of domestic violence. The results showed that Precious depicts various effects of domestic violence, such as loss of self-confidence, anxiety, lack of attention from parents, withdrawal from the family, use of harsh words, stress, depression, self-isolation, not given living expenses, embarrassment to meet others, problematic education, trauma, pregnancy, children replacing parental roles, loss of jobs, imagining the violence experienced, bruises, bumps on the body, and injuries.

Utilizing HPLC-DAD, multivariate analysis, molecular docking, and in vitro validation methods to investigate phenolic compounds with potential antidiabetic properties in Fraxinus mandshurica

This comprehensive study utilized response surface methodology (RSM) to optimize extraction conditions for total flavonoids and phenols from F. mandshurica. Optimal conditions for flavonoids were 75 % ethanol, 63 °C, and 84 min, yielding 14.98 ± 0.13 mg/g. For phenols, it was 70 % ethanol, 66 °C, and 87 min, resulting in 26.04 ± 0.22 mg/g. UPLC-Q-TOF-MS/MS identified 68 constituents in F. mandshurica, categorized into groups. HPLC-DAD established a method for quantitative analysis of 12 target compounds. Geographical distribution analysis highlighted the northeast region as the main production area. Fingerprinting of 30 samples and multivariate analysis identified five active compounds. Hierarchical cluster analysis (HCA) showed differences in active compounds based on origin. In vitro antidiabetic evaluation demonstrated α-glucosidase inhibitory effects. Molecular docking studies were conducted between 12 active compounds and the antidiabetic-related protein 8A27. Esculetin had the highest binding affinity. The study examined extraction methods, analyzed samples, and explored antidiabetic effects, providing data support and insights for F. mandshurica as a natural industrial product and for extracts and active compounds as antidiabetic sources.

The turnover intention of internal controllers of Vietnamese commercial banks in the context of COVID-19

Internal control plays an important role in managing the risks of banking system activities. Controllers are always under pressure from control activities due to the bank’s diverse activities. The resignation of internal controllers is relatively common in commercial banks. It is also a matter of concern for the banking and finance industry, which coordinates economic resources for the country, especially when the internal control period occurs again with the pandemic. The study aims to explore the turnover intention of internal controllers in the context of COVID-19 in Vietnamese commercial banks. The study uses the convenience sampling method to collect data from 339 respondents who are working or worked as internal controllers in commercial banks. The study was surveyed in 2023. The study uses quantitative methods for exploratory factor analysis. Ordinary least squares process the data to test the hypotheses. Some factors affect the turnover intention of internal controllers, such as occupational awareness, employment conditions, office environment, and career opportunities, in the context of COVID-19. The result is a useful resource for researchers to comprehensively understand issues related to the turnover intention of internal controllers in the banking industry. The study supports managers of commercial banks in planning the strategic objectives of human resource stabilization for the stable growth of the banking system.

A method for the quantitative analysis of Lycium barbarum polysaccharides (LBPs) using Fourier-transform infrared spectroscopy (FTIR): From theoretical computation to experimental application

Lycium barbarum polysaccharides (LBPs) is one of the most important active substances in Lycium barbarum (LB). It is a challenge to quantitatively determine the content due to their complex structures and lack of suitable reference standard in practice. In this study, a quantitative analysis method of LBPs in LB was established based on Fourier-transform infrared spectroscopy (FTIR). The stretching vibration of CO on the pyranose ring of saccharide at 921 cm−1 was selected as the characteristic absorption band by theoretical calculation, which can’t be impacted by the preparation methods and interfered by the component monosaccharides. The molecular weight CRM of dextran (Mw 63.3 kDa) served as the reference standard. The introducing internal standard (KSCN) can obtain a good precision (RSD = 1.10 %) and effectively compensate for the analysis errors caused by the environment, quality loss and uneven distribution during the tablet pressing processes. The methodological verification suggested that the method had good accuracy according to the recovery rate (96.61 %–105.45 %) and the blank recovery (92.39 %–99.37 %), respectively. The LOD and LOQ of CRMD were 0.10 mg and 0.32 mg, respectively. The polysaccharide content of LB from 24 different regions (0.50–2.54 %) and 10 batches of LB extracts (7.09–10.56 %) determined by the developed method less than the ones using phenol–sulfuric acid assay (1.95 %–4.83 % for LB and 9.83–15.53 % for extracts, respectively). The established method based on FTIR could be served as a supplement to phenol–sulfuric acid assay and a rapid quantitative assay for polysaccharides products. In additional, this study provided a new idea for the quantitative analysis of plant polysaccharides.

Teachers’ Familiarity, Perceptions, and Training Needs on the Use of ChatGPT in Mathematics Instruction

The rapid development of artificial intelligence (AI) technology has led to a significant change in the way that these tools are used – namely, to improve learning outcomes and teaching approaches in mathematics. This research investigated integrating ChatGPT into mathematics education, focusing on the familiarity, perceptions, and training needs of 110 mathematics teachers in Metro Manila, Philippines. Utilizing a sequential mixed-methods approach, the study used quantitative and qualitative analytical methods to examine teachers' engagement with ChatGPT through online surveys that explored demographic information, usage familiarity, perceptions of its utility in education, and perceived training needs. Findings reveal a widespread unfamiliarity with ChatGPT among educators, alongside a substantial demand for technical and pedagogical training to effectively integrate ChatGPT in teaching. The result also showed that, despite concerns about becoming overly dependent on technology, there is cautious excitement about ChatGPT's ability to improve learning outcomes and engage students. The study contributes to understanding the educational potential of AI in mathematics teaching, highlighting the urgent need for comprehensive professional development programs tailored to equip teachers with the necessary skills and knowledge to integrate AI tools like ChatGPT into the curriculum effectively.

Efficient Elimination of Matrix Effects for the Rapid Screening of β-Agonist Residues in Porcine Urine by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS)

An efficient and innovative clean-up method was developed for the rapid screening of β-agonists residues in porcine urine by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). 1% Aqueous ammonium hydroxide was shown to be effective for the removal of matrix components as a wash step in solid phase extraction (SPE). A significant reduction in matrix effects was observed with values from 81% to 108%. Furthermore, under the optimized conditions, the recoveries of all analytes at 0.3, 0.6, and 3.0 μg/L ranged from 70.1% to 109.0% when employing the external standard method for quantitative analysis, rather than relying on matrix-matched standards or isotope-labelled internal standards to mitigate matrix effects. The limits of detection (LOD) and limits of quantification (LOQ) were 0.1 μg/L and 0.3 μg/L, respectively. This method has been successfully applied to screen β-agonists in porcine urine and is a promising tool for multi-residue analysis.

Sample Preparation for Pharmaceuticals using A Bioanalytical Method: A Review

The process of developing a method that will allow a compound of interest to be located and measured in a biological matrix is known as bioanalytical method development. A substance can frequently be measured using a variety of techniques, and selecting an analytical technique requires careful thought. Different extraction techniques, such as liquid-liquid extraction, solid phase extraction (SPE), and protein precipitation, are used to analyse drugs and their metabolites in a biological matrix. Samples from these extraction methods are spiked with calibration (reference) standards and quality control (QC) samples. The process of establishing if a quantitative analytical method is suitable for biomedical applications is known as bioanalytical method. includes all processes that will be presented measuring analytes quantitatively in particular biological samples such blood plasma, serum, or urine. To evaluate a drug's effectiveness and safety, clinical and non-clinical toxicokinetic and pharmacokinetic studies are used. Therefore, in order to produce accurate results, it is crucial that the applied bioanalytical procedures utilised are thoroughly defined, verified, and documented to a suitable quality. An overview of the development and validation of bioanalytical methods is given in this article, along with key considerations for each stage of method validation. The created procedure is then verified. Evaluation and interpretation of bioavailability, bioequivalence, pharmacokinetic, and pharmacodynamic investigations heavily rely on bioanalytical validations. where various parameters are carried out, including accuracy, precision, selectivity, sensitivity, reproducibility, and stability.

The influence of perceived classroom environment on the learning effectiveness of the association of southeast Asian international students studying in China: The mediating role of learning engagement

This study aims to analyze and comprehend the influence of the classroom environment on the learning effectiveness of the Association of Southeast Asian (ASEAN) students in China especially the significance of learning engagement. Confirmatory factor analysis, descriptive analysis, reliability analysis, correlation analysis and regression analysis were used in this study. 503 useful questionnaires were analyzed through SPSS 22 and AMOS. Both classroom environment cognition and learning engagement could improve ASEAN students' learning effectiveness and learning engagement played a certain mediating effect. The classroom environment and learning engagement are the two primary factors that need to be taken into consideration if ASEAN students in China are to increase their learning efficacy. The more welcoming the classroom environment, the more they can concentrate on participating in various learning activities and the better their learning effectiveness will be. The research object is the ASEAN students in Guangxi Province using the quantitative analysis method of a questionnaire survey. Such as interview methods and other means to further grasp the classroom environment, learning engagement and learning effectiveness of each ASEAN student in China. No studies were conducted on students from ASEAN in other provinces. The cognitive classroom environment can fully affect the learning effectiveness of students from ASEAN countries in China which brings certain theoretical conditions for improving the teaching quality of global students. This study finds out the relationship between classroom environment, learning effectiveness and learning engagement and understands that learning engagement can show a mediating effect which provides certain support for the subsequent in-depth analysis.

REFLECTIONS OF TFRS 16 LEASES STANDARD ON FINANCIAL STATEMENTS APPLICATIONS ON AIRLINE COMPANIES

This study aims to identify reflections of Turkish Financial Report 16 Leases Standard (TFRS 16) on financial statements of two airlines companies. In other words, it was tried to identify changes resulting from TFRS 16 in the study. In the study, quantitative and qualitative analysis methods were used. While quantitative method is ratio analysis, qualitative method is document analysis. To perform the ratio analysis, various assumptions were used. For example, it was assumed floating interest rate was out of the question for lease payments. To perform the analyses, information was obtained from company reports. Prior to performing the ratio analysis, the balance sheets and comprehensive income statements of two prominent airlines in Turkey for the year 2017. Specifically, it focused on converting off-balance sheet liabilities associated with operational leases into capitalised amounts. Based on results of the analyses, it was determined that due to TFRS 16. Based on the TFRS 16, the revised balance sheets show no change in current assets. Non-current assets, current liabilities, and non-current liabilities have all experienced a growth, but equity has decreased. There has been a decline in the cost of sales. This condition has resulted in a rise in gross profit.

A simple and efficient method for betalain quantification in RUBY-expressing plant samples.

The RUBY reporter system has demonstrated great potential as a visible marker to monitor gene expression in both transiently and stably transformed plant tissues. Ectopic expression of the RUBY reporter leads to bright red pigmentation in plant tissues that do not naturally accumulate betalain. Unlike traditional visual markers such as β-glucuronidase (GUS), luciferase (LUC), and various fluorescent proteins, the RUBY reporter system does not require sample sacrifice or special equipment for visualizing the gene expression. However, a robust quantitative analysis method for betalain content has been lacking, limiting accurate comparative analyses. In this work, we present a simple and rapid protocol for quantitative evaluation of RUBY expression in transgenic plant tissues. Using this method, we demonstrate that differential RUBY expression can be quantified in transiently transformed leaf tissues, such as agroinfiltrated Nicotiana benthamiana leaves, and in stable transgenic maize tissues, including seeds, leaves, and roots. We found that grinding fresh tissues with a hand grinder and plastic pestle, without the use of liquid nitrogen, is an effective method for rapid betalain extraction. Betalain contents estimated by spectrophotometric and High-Performance Liquid Chromatography (HPLC) analyses were highly consistent, validating that our rapid betalain extraction and quantification method is suitable for comparative analysis. In addition, betalain content was strongly correlated with RUBY expression level in agroinfiltrated N. benthamiana leaves, suggesting that our method can be useful for monitoring transient transformation efficiency in plants. Using our rapid protocol, we quantified varying levels of betalain pigment in N. benthamiana leaves, ranging from 110 to 1066 mg/kg of tissue, and in maize samples, ranging from 15.3 to 1028.7 mg/kg of tissue. This method is expected to streamline comparative studies in plants, providing valuable insights into the effectiveness of various promoters, enhancers, or other regulatory elements used in transgenic constructs.

Universally Applicable Methods for Comprehensive Risk Assessment of Elemental Impurities in Vitamin A and D Preparations

Background: Vitamin A and D deficiency in children is a common public health problem. In China, almost all children are administered vitamin A and D preparations daily and over the long term. Children are sensitive to elemental impurities, including heavy metals. However, there are no regulatory requirements or reports on the risk assessment of elemental impurities in marketed vitamin A and D preparations. Objective: The aim of this study was to propose an accurate and efficient method suitable for samples from different manufacturers to detect elemental impurities and conduct risk assessments according to the ICH Q3D guidelines. Methods: We developed a universally applicable digestion method for capsules and an ICP–MS method for quantitative analysis of 28 elemental impurities in vitamin A and D formulations. These methods were validated according to the USP 233 guidelines. Elemental impurities in 15 batches of vitamin A and D products from 10 manufacturers and their capsule shells were determined, and risk assessment was conducted according to the International Council for Harmonization Q3D guidelines. Results: All elemental impurities of toxicological concern met the Q3D requirements. However, the concentrations of various elements, including those of lead (not detected to 4.1 µg/g), arsenic (not detected to 7 µg/g), aluminum (not detected to 888 µg/g), and palladium (not detected to 36 µg/g), varied markedly. Moreover, the lead content in one batch from one manufacturer exceeded the control threshold. Conclusion: In this study, we successfully developed an effective digestion method for processing capsules containing samples from different sources and a sensitive ICP–MS method for quantitative determination of 28 elemental impurities in vitamin A and D preparations. ICP–MS should be implemented for the evaluation and control of elemental impurities in vitamin A and D preparations. This will ensure safe long-term vitamin A and D supplementation in children

Post mortem chiral analysis of MDMA and MDA in human blood and hair

Drug-related fatalities in the EU are predominantly associated with opioids. MDMA (Ecstasy) consumption results in fewer lethal intoxications despite its widespread use. This study investigates MDMA-related fatalities, focusing on enantiomer ratios of MDMA and its metabolite MDA to explore the role of metabolism in fatal outcomes. MDMA induces euphoria, increased empathy, and physiological effects such as tachycardia, hypertension, and hyperthermia. Metabolism mainly involves CYP1A2 and CYP2D6, with polymorphism of the latter influencing metabolism rates. Our institute observed several MDMA-related fatalities, which prompted an investigation into the potential role of inefficient drug metabolism in these cases. A novel quantitative chiral analysis method was developed and validated for MDMA, MDA, amphetamine and methamphetamine enantiomers in human blood. Analysis of post mortem blood samples from eleven MDMA-related fatalities exhibited a wide range of concentrations and enantiomer ratios. Variability in R/S MDMA ratios, however, could be linked to the time period of metabolism. Hair analysis revealed high MDMA concentrations in all segments, irrespective of prior drug abuse anamnesis. Therefore, hair analysis may not be suitable for the assessment of past drug use in ecstasy-related fatalities. The results indicated that elevated levels of the MDMA enantiomer are correlated with longer survival times in cases of intoxication. However, there was no clear evidence for slowed MDMA metabolism as a cause of lethal intoxications. While challenges remain due to the diversity of cases, this study contributes valuable insights into ecstasy intoxications, aiding future interpretation of post mortem analysis.

A New Highly Effective Development and Validation of Trastuzumab and Hyaluronidase-Oysk in Bulk and Pharmaceutical Dosage form by Using RP-HPLC Method

A simple, rapid, precise, sensitive, and reproducible Reverse phase High-performance liquid chromatography (RP-HPLC) method has been developed for the quantitative analysis of Trastuzumab and Hyaluronidase-OYSK in the pharmaceutical dosage form. Chromatographic separation of Trastuzumab and Hyaluronidase-OYSK was achieved on Waters Alliance-e2695by using Agilent Eclipse XDB (250x 4.6mm, 5µ) column and the mobile phase containing Acetonitrile: Ammonium formate pH-3.0/OPA in the ratio of 30:70% v/v. The flow rate was 1.0 ml/min; detection was carried out by absorption at 228nm using a photodiode array detector at ambient temperature. The number of theoretical plates and tailing factor for Trastuzumab and Hyaluronidase-OYSK were NLT 2000 and should not be more than 2 respectively. % Relative standard deviation of peak areas of all measurements always less than 2.0. The proposed method was validated according to ICH guidelines. The method was found to be simple, economical, suitable, precise, accurate & robust method for quantitative analysis of Trastuzumab and Hyaluronidase-OYSK study of its stability.