Pseudomonas fluorescens biovars were isolated from Oreochromis niloticus collected from Qaroun Lake fish farms, Egypt. Microorganisms were added to basal diet with 30 % protein to assess their probiotic properties on growth-performance, hematological parameters, enzymatic activities and survival rate. Tilapia (2.93 ± 0.22 g) were distributed into four equal groups, three replicates each. First group were fed on basal diet as control group, 2 nd , 3 rd and 4 th groups were fed on diet supplemented with Pseudomonas fluorescens biovar I, II & III respectively; fish were fed twice daily at the rate of 5-7 % of their body weight for 45 days. The groups were challenged intra-peritoneal with Aeromonas hydrophila (1.8 x 10 8 CFU ml −1 ) and observed for 7 days. The growth rate was significantly higher in groups fed on Pseudomonas fluorescens in the diet than control group. Hematological and non-specific immune parameters were significantly higher than control group. The highest protein, globulin, glucose, Plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenases activity were observed at 3 rd group which fed on Pseudomonas fluorescens biovar II. The challenged fish exhibited lowest cumulative mortality rate in 3 rd group (10%) compared to control group (90%). So, it could be concluded that non-pathogenic pseudomonas isolates from marine or brackish water could confer beneficiary effects for Nile tilapia through improving growth performance, immunity enhancement and resistance against diseases.
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