Pumpkin Seed Oil Research Articles

Influence of Wall Material Composition on Microencapsulation Efficiency of Cold Pressed Pumpkin Seed Oil by Freeze-Drying

Influence of Wall Material Composition on Microencapsulation Efficiency of Cold Pressed Pumpkin Seed Oil by Freeze-Drying

Encapsulation of Pumpkin Seed Oil in Alginate Capsules

Encapsulation of Pumpkin Seed Oil in Alginate Capsules

AMELIORATIVE EFFECT OF PUMPKIN SEED OIL AGAINST THE BISPHENOL-A ADVERSE EFFECTS IN MALE MICE.

The present study was conducted to evaluate the ameliorative role of pumpkin seed oil (PSO) against potential adverse effects of bisphenol-A (BPA) in male mice. BPA was administered to the mice orally at a dose of 50 mg/kg body weight once a day for 28 successive days. While, PSO was administered to the mice orally at 1 mL/kg b.w. either before, with or after treatment of BPA, once a day for 28 successive days. The studied parameters were DNA damage evaluation using comet assay in liver and testes cells and micronucleus test in bone marrow; and histopathological examination of liver and testes tissues. Results revealed that BPA induced DNA damage in tested cells and marked histopathological alterations in liver and testes. In contrast, PSO treatments alleviated DNA damage and improved the histopathological alterations in liver and testes tissues. Furthermore, administration of mice with the PSO before BPA treatment was the best regimen in the alleviation of the adverse effects of BPA, followed by administration of PSO after then with treatment of BPA. It can be concluded that PSO may has a protective role against BPA genotoxicity and histopathological alterations in male mice.

PROTECTIVE EFFECT OF PUMPKIN SEED OIL AGAINST LEAD ACETATE TOXICITY IN MALE MICE

The goal of the present work was to investigate the protective effect of pumpkin seed oil (PSO) against lead acetate toxicity in male mice. lead acetate (20 mg/kg b.w.) was administered orally to mice once a day for 30 successive days. Whereas, PSO was administered to the mice orally at 1.5 mL/kg b.w. once a day for 30 successive days concurrently with treatment of lead acetate. The studied parameters were relative organs weights, DNA damage evaluation using comet assay in liver and kidney cells and micronucleus test in bone marrow and biochemical assessment of the liver and kidney function. Results showed that, lead acetate caused DNA damage in tested cells and significant increase in the levels of biochemical measurements. In contrast, PSO administration plus lead acetate effectively alleviated DNA damage in tested cells and improved the biochemical alterations. It can be concluded that PSO may has a protective role against lead acetate toxicity in male mice.

Trans-perineal pumpkin seed oil phonophoresis as an adjunctive treatment for chronic nonbacterial prostatitis.

BackgroundA significant number of men who are younger than 50 years visit urologists for interminable prostatitis. This study aimed to thoroughly investigate the effect of pumpkin seed oil (PSO) phonophoresis on chronic nonbacterial prostatitis (CNBP).Subjects and methodsSixty patients with CNBP were randomly assigned to three groups: Group A, wherein patients were treated with PSO using phonophoresis; Group B, where patients underwent transperineal continuous low-intensity ultrasound (LIUS); and Group C, wherein patients underwent placebo LIUS. All three groups received their corresponding treatments daily for up to 3 weeks. The NIH-Chronic Prostatitis Symptom Index (NIH-CPSI), residual urine determined by urodynamic measurements, and flow rate were used to analyze study outcomes. The white blood cell (WBC) count in the prostatic secretion was determined.ResultsComparisons of the intragroup mean values of all measurements in Groups A and B before and after the end of the treatment showed a significant improvement in residual urine, flow rate, WBC count, and NIH-CPSI (p < 0.05), whereas no significant change was found in Group C (p > 0.05). Between-group comparisons of all variables showed a significant difference was found after intervention (p < 0.05). Postintervention comparisons between Groups A and B showed a significant difference in all measurements, except for WBC, in favor of Group A. Comparing the changes between Groups A and C, a significant difference was found in all measurements (p < 0.05). Furthermore, all parameters differed significantly when comparing Groups B and C (p < 0.05).ConclusionThe current study showed that PSO phonophoresis can produce a significant effect in the management of CNBP and can, therefore, be considered a safe, noninvasive method for the treatment of CNBP.

New cosmetic formulations with broad photoprotective and antioxidative activities designed by amaranth and pumpkin seed oils nanocarriers

Plant oils are widely recognized for their beneficial effects in the cosmetic industry, possessing many biological activities. These natural oils provide multiple advantages such as superior antioxidant, nutraceutical, anti-aging, anti-inflammatory, antimicrobial and emollient properties. This study aims to consider the great importance of the various fractions of plant oils in the development of innovative sunscreen formulations based on nanostructured lipid carriers (NLCs) which act as delivery systems for three antioxidant and anti-UV bioactives. Amaranth oil (AO) – Amaranthus cruentus and pumpkin seed oil (PSO) – Cucurbita seed spp were fitted in the lipid NLCs core, forming new delivery systems able to simultaneously entrap two UVA and UVB filters (diethylamino hydroxybenzoyl hexyl benzoate – DHHB, 2-ethylhexyl salicylate – EHS), and an antioxidant (hesperidin – HES). The dimensional investigation showed that the type of vegetable oil had no significant influence on NLC size, the main diameters being ranged between 110 and 125 nm. The evaluation of physical stability during 3 months has revealed extremely electronegative zeta potential values, e.g. between −44 and −54 mV, reflecting an excellent physical stability. A unique feature of the developed nano-delivery systems is that they provide a differentiated release of the three active principles, e.g. a sustained release of the natural antioxidant versus maintaining significant amounts of UVA and UVB filters in the formulation. The antioxidant properties, evaluated by two in vitro techniques, showed that NLCs exhibit a high percent of short-time life radical inhibition, respectively 93–98%. The most effective scavenging system of oxygen free radicals has proved to be the NLC prepared with AO that contains 82% Squalene, while a better ability to inhibit ABTS cation radicals was detected for NLC prepared with an amaranth fraction of 34% Squalene. The entrapment process had a great influence on the UV-absorptive properties, the SPF and UVA-PF values being remarkable, even the amount of UV filters was extremely reduced in comparison with that one existent in commercial creams; the hydrogels based on NLC-EHS-DHHB-HES assure an absorption of 99% UVB radiations (SPF = 46/50.5) and have an ability tocombat 83% of UVA radiation (UVA-PF ranging between 13.9 and 15.5). In conclusion, this study provides an innovative and non-invasive design of herbal cosmetic formulations with superior photoprotection and enhanced antioxidant properties that could reduce the occurrence of skin cancer and delay the process of photoaging.

Quality evaluation of biscuits produced from composite blends of pumpkin seed oil press cake and wheat flour

AbstractPumpkin seed oil press cake (PSOPC) is a by‐product of pumpkin oil production, and after cold pressing, a significant amount of oil still remains in the press cake. The aim of this study was to investigate the possible use of PSOPC flour as a substitute for wheat flour in biscuit production and, consequently, for reducing shortening usage. Biscuits have been produced from composite blends of plain white flour and PSOPC flour in ratios 100:0, 80:20, 60:40 and 40:60, respectively. Shortening addition was gradually reduced depending on the PSOPC flour addition. Biscuit baking quality was determined by width, thickness, spread factor, volume and textural properties. Computer vision was used to evaluate biscuit colour, and sensory evaluation was conducted for colour, taste, texture and overall acceptance. Results showed that PSOPC flour decreased biscuit diameter, height and volume and also caused a softer texture of biscuit. PSOPC flour contributed to the greenish colour of biscuits and the pleasant taste of roasted pumpkin seed. PSOPC flour can be successfully used as a functional and nutritionally valuable substitute for wheat flour and shortening, even in quantities up to 60%, without significant deterioration of the technological quality of biscuits.

Effect of Chemical Interesterification on the Physicochemical Characteristics and Fatty Acid Profile of Bakery Shortening Produced from Shea Butter and Fluted Pumpkin Seed Oil Blend

The objective of this work was to evaluate the effect of chemical interesterification, as a modification process on the physicochemical properties and fatty acid profile of bakery shortening produced from a blend of Shea stearin and fluted pumpkin seed oil. The Shea nut oil was fractionated, and the solid stearin fraction blended with fluted pumpkin seed oil in the following ratios: 30:70, 40:60, 50:50,and 60:40 (Shea stearin: fluted pump seed oil), The blends were stabilized with recommended additives such as; distilled monoglyceride (E471) and preservatives such as; citric acid (E330), BHT (E321), homogenized by continuous stirring and crystalized by cooling at 17°C, then tempered at 23 – 25°C for 48h, to attain a stable polymorphic form, used as non-interesterified blends (NIEBs). Another set of 30:70; 40:60; 50:50; and 60:40 (Shea stearin: fluted pumpkin seed oil), were chemically interesterified with sodium methoxide (CH3ONa) as catalyst, crystalized, stabilized, and used as chemically interesterified blends (CIEBs).The chemical properties, physical characteristics (including solid fat content), and the fatty acid profile of all the chemically interesterified (CIEB) and the non-interesterified (NIEB) samples were determined. There was no significant (p>0.05) change in the peroxide value, iodine value, and the refractive index of NIEBs and there corresponding CIEBs. The acid value, free fatty acids, slip melting point, density, and solid fat content were significantly (p<0.05) reduced after interesterification. Interesterification caused rearrangement of triacylglycerol species, resulting in increased unsaturated fraction and reduction in the saturated fraction. Interesterification gave fats with wide plastic range suitable for bakery fats, icing-shortening, filler fats, and all-purpose shortenings.

Optimization of simultaneous determination of metals in commercial pumpkin seed oils using inductively coupled atomic emission spectrometry

In this work, the inductively coupled plasma atomic emission spectrometry (ICP-AES) was developed and validated for determination of 27 metals in nine pumpkin oils from two production processes (cold pressed and roasted). A microwave assisted digestion was applied for the dissolution of the samples. To evaluate plasma robustness and analytical performance, the Mg II 280.270 nm/Mg I 285.213 nm (Mg II/Mg I) line intensity ratio was used. The robust plasma conditions in axial/radial view mode were reached at an RW power of 1150 W and an argon nebulizer flow rate of 0.5 L/min. The method is evaluated by application of the standard addition method and by recovery test. In general, the recoveries for all elements in pumpkin seed oils were between 90% and 110% except, only for Cd, Pb and As they were <90% and >110%. The most abundant element is K followed by, Mg, P, Ca and Na. Pumpkin seed oils were also found to be a good source of Fe, Zn, Cu and Mn. The highest levels of analyzed elements were found in pumpkin seed oils producing from the roasted pumpkin seed paste. In all samples, the content of heavy metals was below the recommended tolerable levels proposed by Joint FAO/WHO Expert Committee on Food Additives and does not a pose a health risk for the consumer. The levels of the metals in pumpkin seed oils were also compared with those reported by the literature. Cluster analysis (CA) of the analytical data confirmed the observed differences between oils from two production processes.

Schistosomicidal, antifibrotic and antioxidant effects of Cucurbita pepo L. seed oil and praziquantel combined treatment for Schistosoma mansoni infection in a mouse model.

Despite the seriousness of schistosomiasis, its treatment depends only on praziquantel (PZQ), which has begun to lose its efficacy against the emergent Schistosoma mansoni-resistant strains. Therefore, the discovery of a novel schistosomicidal drug is an urgent priority. This study was designed to evaluate treatment with Cucurbita pepo L. (pumpkin) seed oil (PSO) alone and combined with PZQ against S. mansoni in experimentally infected mice. The study involved five groups: GI was the normal control; GII was the infected control; GIII was treated with an oral dose of PZQ of 500 mg/kg/day for two successive days, starting in the sixth week post infection; GIV was treated with an oral dose of PSO of 50 mg/kg/day for four weeks, starting in the fourth week post infection; and GV was treated with combined PSO-PZQ. Worm burden, tissue egg load and oogram pattern were estimated, and the ultrastructure alterations were examined. Histopathological examination of granuloma diameters, collagen deposition (Picro Sirius red stain), and angiogenesis (immunohistochemical expression of CD34+) was conducted and serum liver enzymes were measured to assess the liver condition. Moreover, the oxidative stress was evaluated by determining the amounts of malondialdehyde and superoxide dismutase in liver homogenates. The results revealed significant changes in all the assessed parameters with PSO administration. However, PZQ was significantly more effective as an antiparasitic agent, whereas PSO was better in terms of fibrosis and oxidative stress. The most significant results were obtained in group V, which may be attributed to a synergy between PZQ and PSO, with antiparasitic, antioxidant and antifibrotic properties.

Cold-pressed pumpkin seed (&lt;em&gt;Cucurbita pepo&lt;/em&gt; L.) oils from the central Anatolia region of Turkey: Characterization of phytosterols, squalene, tocols, phenolic acids, carotenoids and fatty acid bioactive compounds

There is a growing interest in cold-pressed oils because they present high contents of bioactive compounds. These oils have the characteristic properties of seeds and are specific products of their regions. The aim of this study was to determine the compositions and contents of fatty acids, phytosterols, squalene, tocols, phenolic acids, carotenoids and phenolic bioactives, and the free radical scavenging as well as antioxidant activities of cold-pressed pumpkin (Cucurbita pepo L.) seed oils. Oil samples from raw pumpkin seeds cultivated in four different central Anatolia regions of Turkey were prepared using a laboratory screw-pressing machine. The results indicate that cold-pressed pumpkin seed oils (PSO) have excellent quality with high contents of polyunsaturated fatty acids (%ΣPUFAs) (53.60 ±0.06-53.73 ±0.05), total phytosterols (782.1±9.7–805.2 ±11.3 mg/100 g oil), squalene (591.3±10.6–632.5±11.4 mg/100 g oil), tocols (97.79 ±0.76?94.29 ±0.34 mg/100 g oil), phenolic acids (22.73 ±0.41–23.98 ±0.46 mg/100 g oil), carotenoids (6.95 ±0.03–7.60 ±0.03 mg/100 g oil), total phenolics (3.96 ±0.13 –5.82±0.15 mg GAE/100 g), free radical-scavenging activity (5.70 ±0.13?7.35 ±0.15 mg GAE/100 g) and total antioxidant activity (26.67±0.97-38.89±1.41 mg GAE/100 g) values. Thus, this study demonstrates that the cold- pressed PSOs from the central Anatolia regions of Turkey are an excellent source of natural bioactive compounds, free of chemical contaminants and nutritious.

Cucurbita maxima Pumpkin Seed Oil: from the Chemical Properties to the Different Extracting Techniques

Pumpkin seed oils are rich in bioactive compounds such as tocopherols, sterols, β-carotene, and lutein that have, along with some fatty acids, high nutritional value factors. In addition, it has so far been proven that these compounds have a positive effect on human health. The present study mainly aimed at evaluating the chemical composition and the bioactive compounds of pumpkin (Cucurbita maxima) seed oil of the ‘Béjaoui’ Tunisian cultivar using both cold pressing and solvent extraction methods. The seed oils contained substantial amounts of unsaturated fatty acids, particularly oleic and linoleic acids, with values ranging respectively from 28.19% for cold pressed pumpkin seed oil to 30.56% for pumpkin seed oil extracted by pentane and from 43.86% for pumpkin seed oil extracted by pentane to 46.67% for cold pressed pumpkin seed oil of the total amount of fatty acids. Investigations of different seed oils revealed that extraction techniques had significant effects on the antioxidant activity and the γ-tocopherol. Cold pressed pumpkin seed oil revealed the highest γ-tocopherol content (599.33 mg kg-1) and the highest oxidative stability (3.84 h). However, the chloroform/methanol extracted pumpkin seed oil, which is rich in total phenolics (54.41 mg Gallic Acid Equivalent kg-1), was a more effective scavenger of DPPH radicals (250 µmoles Trolox kg-1) than the poor phenolic pumpkin seed oils extracted by hexane and pentane (110 µmoles Trolox kg-1 vs 100 µmoles Trolox kg-1). Based on its unique seed oil features, cold press extracted pumpkin seed may add great value to product innovation in the industrial, nutritional, cosmetic, and pharmaceutical fields.

Improved effect of pumpkin seed oil against the bisphenol-A adverse effects in male mice.

The present study was conducted to evaluate the ameliorative role of pumpkin seed oil (PSO) against potential adverse effects of bisphenol-A (BPA) in male mice. BPA was administered to the mice orally at a dose of 50 mg/kg body weight once a day for 28 successive days. While, PSO was administered to the mice orally at 1 mL/kg b w either before, with or after treatment of BPA, once a day for 28 successive days. The studied parameters were DNA damage evaluation using comet assay in liver and testes cells and micronucleus test in bone marrow; and histopathological examination of liver and testes tissues. Results revealed that BPA induced DNA damage in tested cells and marked histopathological alterations in liver and testes. In contrast, PSO treatments alleviated DNA damage and improved the histopathological alterations in liver and testes tissues. Furthermore, administration of mice with the PSO before BPA treatment was the best regimen in the alleviation of the adverse effects of BPA, followed by administration of PSO after then with treatment of BPA. It can be concluded that PSO may has a protective role against BPA genotoxicity and histopathological alterations in male mice.

Comparative Assessment of the Physicochemical Properties and Fatty Acid Profile OF Fluted Pumpkin Seed Oil with Some Commercial Vegetable Oils in Rivers State, Nigeria

Comparative Assessment of the Physicochemical Properties and Fatty Acid Profile OF Fluted Pumpkin Seed Oil with Some Commercial Vegetable Oils in Rivers State, Nigeria

Prospective Evaluation of Pumpkin Seed Oil as an Alternative Fuel - A Novel Experimentation

Prospective Evaluation of Pumpkin Seed Oil as an Alternative Fuel - A Novel Experimentation

Changes in Physicochemical Characteristics and Microbiological Quality of Bakery Shortening Formulated with Shea Butter and Fluted Pumpkin Seed Oil During Storage

The objective of this work was to evaluate the changes that occur in physicochemical characteristics and microbiological qualityof bakery shortening formulated with Shea stearin and fluted pumpkin seed oil blends during storage, so as to ascertain its storage stability. Edible oils were extracted from Shea nuts ( Vitellaria paradoxa Geartner) and Fluted pumpkin ( Telfairia occidentalis Hook ) seeds. The extracted Shea butter and fluted pumpkin seed oil samples were refined and modified using two methods namely fractionation and chemical interesterification to produce bakery fats. The Shea butter was fractionated, and the solid stearin fraction blended with fluted pumpkin seed oil in the following ratios: 30:70, 40:60, and 50:50, (Shea stearin: fluted pump seed oil), The blends were stabilized with recommended additives such as; distilled monoglyceride (E471) and preservatives such as; citric acid (E330), BHT (E321), and sodium benzoate (E211), homogenized by continuous stirring and plasticized by chilling to 17°C, then tempered at 23 – 25°C for 48h, to attain a stable polymorphic form, used as fractionated blends (FRBs). Another set of 30:70; 40:60; and 50:50 (Shea stearin: fluted pumpkin seed oil), were chemically interesterified with sodium methoxide (CH3ONa) as catalyst, crystalized, stabilized, and used as chemically interesterified fractionated blends (CIEFBs). The products were packed and sealed in opaque plastic cups and stored at room temperature (28±2°C) for 90 days. Changes in physicochemical properties (including solid fat content) and microbiological quality (yeast and moulds count) were determined. Peroxide value (PV) and acid value (AV) of the bakery fats formulated with fractionated blends increased slightly after 90 days of storage. There were no significant (P>0.05) increase in PV of the interesterified samples after storage for 90 days. No significant change was recorded in the iodine value, slip melting point, and smoke point of the products on storage, except the 30:70 fractionated blend which reduces in iodine value (IV) and smoke point from 94.60g/100g to 92.50g/100g, and 200.30°C – 198.00°C, respectively. The products were microbiologically stable throughout the period of storage.

Potencijal izolata proteina uljane pogače bundeve u kreiranju hleba od prosa

Potencijal izolata proteina uljane pogače bundeve u kreiranju hleba od prosa

Innovative technology pumpkin oil production

Innovative technology pumpkin oil production

Effect of Supplementation Different Levels of Vitamin E and Pumpkin Seed Oil to the Diet on Productive, Physiological and Reproductive Performance of Japanese Quail

The principal goal of this study was to evaluate whether dietary vitamin E and pumpkin seed oil would affect the productive, physiological and reproductive performance of Japanese quail by using 120 birds at 22 weeks of age. The birds were randomly distribution into five dietary groups 24 birds each group contain three replicated pens, as follow: First group: kept on basal diet without any addition as control group, second and third groups were fed the basal diet supplemented with 150 and 200 mg/ kg vitamin E / kg of diet, fourth and fifth groups were fed the basal diet supplemented with 15 and 30 ml/kg pumpkin seed oil /kg of diet respectively. The results showed a significant improvement in egg production (H.D%), egg number, egg weight and egg mass produced for the vitamin E (200mg/ kg of diet) compared with the other groups. Additionally, the results showed that vitamin E (200mg/ kg of diet) significantly increased gonads relative weight , testes development, egg characterizes and serum testosterone, estrogen, FSH and LH hormones compared with other groups. Also , the birds were fed on 15 and 30 ml seed pumpkin oil/ kg of diet were significantly increased in the most parameter compared with control group. It can be concluded that the addition of vitamin E (200 mg/kg of diet) and pumpkin seed oil (30ml of diet), had positive effects on gonads, testicular histology, egg production and serum testosterone, estrogen, FSH and LH hormones concentration.

Effect of Supplementation Different Levels of Vitamin E and Pumpkin Seed Oil to the Diet on Productive, Physiological and Reproductive Performance of Japanese Quail

&#x0D; The principal goal of this study was to evaluate whether dietary vitamin E and pumpkin seed oil would affect the productive, physiological and reproductive performance of Japanese quail by using 120 birds at 22 weeks of age. The birds were randomly distribution into five dietary groups 24 birds each group contain three replicated pens, as follow: First group: kept on basal diet without any addition as control group, second and third groups were fed the basal diet supplemented with 150 and 200 mg/ kg vitamin E / kg of diet, fourth and fifth groups were fed the basal diet supplemented with 15 and 30 ml/kg pumpkin seed oil /kg of diet respectively. The results showed a significant improvement in egg production (H.D%), egg number, egg weight and egg mass produced for the vitamin E (200mg/ kg of diet) compared with the other groups. Additionally, the results showed that vitamin E (200mg/ kg of diet) significantly increased gonads relative weight , testes development, egg characterizes and serum testosterone, estrogen, FSH and LH hormones compared with other groups. Also , the birds were fed on 15 and 30 ml seed pumpkin oil/ kg of diet were significantly increased in the most parameter compared with control group. It can be concluded that the addition of vitamin E (200 mg/kg of diet) and pumpkin seed oil (30ml of diet), had positive effects on gonads, testicular histology, egg production and serum testosterone, estrogen, FSH and LH hormones concentration.&#x0D;