Pulegium Extract Research Articles

Mentha pulegium ekstraktı güçlü in vitro antimikrobiyal ve sitotoksik etki göstermiştir

Mentha pulegium is a medicinally important and well-known plant and used for various purposes such as medicinal, nutritional and spice. We are analyzed to observe the antimicrobial, antioxidant and cytotoxic properties of M. pulegium extracts in this study. The antimicrobial activity of M. pulegium was tested using the agar well method. MIC, MBC and antimicrobial activity were tested on Staphylococcus aureus (ATCC 25923), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25322), Bacillus megaterium (DSM32) and Candida albicans (FMC17) microorganisms. Clindamycin (2 µg) and Amoxicillin (30 µg) were used as positive control and Dimethylsulfoxide (DMSO) as negative control. Cytotoxic activity of extracts at different concentrations obtained from solvents such as acetone, chloroform and methanol using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test method; µg/ml cytotoxic activity and cell death patterns after exposure to different concentrations of the extracts on human breast cancer (MDA-MB-231), human pancreatic cancer (PANC1), human ovarian cancer (OVCAR3) and human lung cancer (A549) cell lines determined accordingly. As a result, it was determined that M. pulegium extract has a strong antimicrobial activity spectrum and cytotoxic effect.

Six Algerian plants: Phenolic profile, antioxidant, antimicrobial activities associated with different simulated gastrointestinal digestion phases and antiproliferative properties

IntroductionMediterranean countries, including Algeria, have medicinal plants used in folk medicine, such as Artemisia campestris L., Artemisia herba-alba Asso, Juniperus phoenicea L., Juniperus oxycedrus L., Mentha pulegium L., and Lavandula angustifolia Mill. It is noteworthy that the bioaccessibility of their polyphenolic ingredients have not been critically evaluated before. MethodsThe bioactive properties of the aqueous extracts of Artemisia campestris L., Artemisia herba-alba Asso, Juniperus phoenicea L., Juniperus oxycedrus L., Mentha pulegium L., and Lavandula angustifolia Mill. were investigated. They were subjected to a simulated gastrointestinal digestion to evaluate the antioxidant, antimicrobial activities and total polyphenol contents. Their antiproliferative activities against three human cancer cell lines (MCF-7, T47D, and Caco-2) were also performed using the aqueous extracts. The phenolic profile was also studied by reversed-phase high-performance liquid chromatography coupled to ultraviolet spectrometry detector (RP-HPLC-UV). ResultsThe results indicated that bioaccessibility of total phenolic compounds after the post-gastric, intestinal, and colon phases was in the ranges of 4.34–11.57 %, 0–0.55 %, and 3.92–9.24 %, respectively when compared with their aqueous extracts. Intestinal and colon phase samples collected from intestinal digestion of six plant extracts did not have any antioxidant activity. Juniperus spp. extracts had the highest antiproliferative activity compared to the results obtained from other tested species. ConclusionsThe high antioxidant and cytotoxic activities of Juniperus samples may be attributed to the higher levels of total phenols, as well as the catechins, rutin and caffeic acid, whilst M. pulegium extract with the lowest phenolic content and lower amounts of these metabolites, having p-coumaric acid as a predominant phenolic compound, was shown to be less effective as an antioxidant and anticancer agent.

Bioengineered synthesis of phytochemical-adorned green silver oxide (Ag2O) nanoparticles via Mentha pulegium and Ficus carica extracts with high antioxidant, antibacterial, and antifungal activities

Silver oxide nanoparticles have various biomedical and pharmaceutical applications. However, conventional nanofabrication of Ag2O is associated with the use of toxic chemicals and organic solvents. To circumvent this hurdle, herein silver oxide quantum dots (Ag2O-QDs) were synthesized quickly (3 min) via the use of ultrasonic irradiation and plant-extract. Additionally, due to ultrasonic irradiation's effect on cell-wall destruction and augmentation of extraction efficiency, ultrasonic was also used in the preparation of Mentha pulegium and Ficus carica extracts (10 min, r.t) as natural eco-friendly reducing/capping agents. The UV–Vis result indicated a broad absorption peak at 400–500 nm. TEM/SEM analysis showed that ultrasound introduced a uniform spherical particle and significantly reduced particle size compared to the conventional heating method (∼ 9 nm vs. ∼ 100 nm). Silver and oxygen elements were found in the bio-synthesized Ag2O by EDS. The FTIR and phenol/flavonoid tests revealed the presence of phenol and flavonoid associated with the nanoparticles. Moreover, nanoparticles exhibited antioxidant/antibacterial/antifungal activities. The MIC and MBC results showed the Ag2O QDs synthesized with M. pulegium extract have the highest antibacterial activity against E. coli (MBC = MIC:15.6 ppm), which were significantly different from uncoated nanoparticles (MBC = MIC:500 ppm). The data reflects the role of phyto-synthesized Ag2O-QDs using ultrasonic-irradiation to develop versatile and green biomedical products.

Green synthesis of multifunctional ZnO/chitosan nanocomposite film using wild Mentha pulegium extract for packaging applications

Following the global corona virus pandemic and environmental contamination caused by chemical plastic packaging, awareness of the need for environmentally friendly biofilms and antibacterial coatings is increasing. In this study, a biodegradable hybrid film, comprising of green-synthesized zinc oxide nanoparticles (ZnO NPs) with a chitosan (CS) matrix, was fabricated using a simple casting procedure. The ZnO NPs were synthesized using wild Mentha pulegium extract, and the synthesized NPs and films were characterized using different approaches. The structural, morphological, mechanical, antibacterial, and optical properties, as well as the hydrophilicity, of the prepared samples were investigated using various techniques. Gas chromatography-mass spectrometry measurements revealed the presence of phenolic compounds in the M. pulegium extract. In addition, a strong coordination connection between Zn2+ and the chitosan matrix was confirmed, which resulted in a good dispersion of ZnO in the chitosan film. The surface of the composite films was transparent, smooth, and uniform, and the flexible bio-based hybrid films exhibited significant antibacterial and antioxidant characteristics, strong visible emission in the 480 nm region, and UV-blocking properties. The ZnO/CS films displayed a potential to extend the shelf life of fruits by up to eight days when stored at 23°C, and also acted as an acceptable barrier against oxygen and water. The biodegradable ZnO/CS film is expected to keep fruit fresher than general chemical plastic films and be used for the packaging of active ingredients.

Molecular Interaction Studies and Phytochemical Characterization of Mentha pulegium L. Constituents with Multiple Biological Utilities as Antioxidant, Antimicrobial, Anticancer and Anti-Hemolytic Agents.

Multiple biological functions of Mentha pulegium extract were evaluated in the current work. Phytochemical components of the M. pulegium extract were detected by Gas Chromatography-Mass Spectrometry (GC-MS) and High-performance liquid chromatography (HPLC). Moreover, M. pulegium extract was estimated for antioxidant potential by 2,2-Diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical scavenging, antimicrobial activity by well diffusion, and anticoagulant activity via prothrombin time (PT) and activated partial thromboplastin time (APTT). GC-MS analysis detected compounds including cholesterol margarate, stigmast-5-en-3-ol, 19-nor-4-androstenediol, androstan-17-one, pulegone-1,2-epoxide, isochiapin B, dotriacontane, hexadecanoic acid and neophytadiene. Chrysoeriol (15.36 µg/mL) was followed by kaempferol (11.14 µg/mL) and 7-OH flavone (10.14 µg/mL), catechin (4.11 µg/mL), hisperdin (3.05 µg/mL), and luteolin (2.36 µg/mL) were detected by HPLC as flavonoids, in addition to ferulic (13.19 µg/mL), cinnamic (12.69 µg/mL), caffeic (11.45 µg/mL), pyrogallol (9.36 µg/mL), p-coumaric (5.06 µg/mL) and salicylic (4.17 µg/mL) as phenolics. Antioxidant activity was detected with IC50 18 µg/mL, hemolysis inhibition was recorded as 79.8% at 1000 μg/mL, and PT and APTT were at 21.5 s and 49.5 s, respectively, at 50 μg/mL of M. pulegium extract. The acute toxicity of M. pulegium extract was recorded against PC3 (IC50 97.99 µg/mL) and MCF7 (IC50 80.21 µg/mL). Antimicrobial activity of M. pulegium extract was documented against Bacillus subtilis, Escherichia coli, Pseudomonas aureus, Candida albicans, Pseudomonas aeruginosa, but not against black fungus Mucor circinelloides. Molecular docking was applied using MOE (Molecular Operating Environment) to explain the biological activity of neophytadiene, luteolin, chrysoeriol and kaempferol. These compounds could be suitable for the development of novel pharmacological agents for treatment of cancer and bacterial infections.

Generation and Characterization of Silver Nanoparticles in Mentha pulegium Extract and Evaluation of Biological Activities of the Prepared Extract

The aim of the present study was to synthesize silver nanoparticles (AgNPs) using Saudi Mentha pulegium leaves, to characterize the physicochemical properties of the resulting AgNPs, and to evaluate the biological activities of the resulting AgNP‐containing extract. The formation of AgNPs in M. pulegium extract was indicated by a change in color following the addition of silver nitrate and was confirmed using UV‐visible spectroscopy with a maximum absorbance at 425 nm. Energy dispersive X‐ray spectroscopy (EDX) indicated that the anisotropic AgNPs were spherical, and Fourier transform infrared spectroscopy (FTIR) spectral analysis indicated that the aqueous M. pulegium extracts were responsible for reducing Ag+ to Ag0. The secondary metabolite contents of the methanolic M. pulegium extract corresponded to 17 mg GAE/g DW. DPPH and ABT radical‐scavenging assays indicated that the M. pulegium extracts possessed antioxidant activity (IC50 = 6 and 3 μg/mL, respectively). Disc and broth dilution assays revealed that the extracts exerted significant antibacterial activity, with the inhibition zone diameters and minimal inhibition concentrations of 17–24 mm and 0.08–0.62 mg/mL, respectively. These findings clearly indicate that modified plant extracts have high biological importance and potential use as preservatives in the pharmaceutical and food industries.

Green Fabrication of Bioactive Silver Nanoparticles Using Mentha pulegium Extract under Alkaline: An Enhanced Anticancer Activity.

Fabrication of silver nanoparticles (AgNPs) using Chinese herbal medicine is popular as the bioactive components included in them would generate potential synergistic effect with the metal nanoparticles. The leaf of Mentha pulegium, whose extract contains a range of phytochemicals and exhibits a wide spectrum of bioactivities, is used as Chinese herbal medicine after drying naturally. Thus, the green synthesis of AgNPs using Mentha pulegium has aroused interests from analysts. However, the biosynthesis of AgNPs under alkaline conditions and the biological activities remain elusive, where alkaline conditions may influence the physicochemical properties and the biological activities of biosynthesized AgNPs. In this study, we were stimulated to fabricate bioactive AgNPs using Mentha pulegium extract under alkaline conditions, accompanied by a systematic evaluation on the effect of biosynthesis parameters on the formation, average size, and polydispersity of AgNPs. Our results showed that alkaline conditions could accelerate the formation of AgNPs with a small average size but at a disadvantage to the polydispersity. Additionally, the as-prepared AgNPs had a hexagonal structure and spherical shape with an average size of 15.7 ± 0.1 nm, existing in the monodispersed form and revealing a high degree of stability. The AgNPs exhibited potent antioxidant and significant inhibitory activity for both bacterial and cancer cell lines. The MIC values of AgNPs for Staphylococcus aureus and Escherichia coli were both 50.0 μg·mL–1, and the IC50 values for HCT116, HepG2, and HeLa cells were 9.0, 14.5, and 31.5 μg·mL–1, respectively. The AgNPs biosynthesized using M. pulegium under alkaline conditions, which had a smaller size and more surface loads, are entirely different with those synthesized under acidic conditions, and the anticancer activity increased significantly. The internalization of AgNPs inside these five cells displayed a variant trend with variable AgNPs concentrations, suggesting the different mechanism of cell death. For two pathogens, HCT116 and HepG2 cancer cell lines, both cell wall and intracellular damage may be responsible for the cell death. However, for Hela cell line the cell death may be rooted in oxidative stress or intracellular penetration. These results confirmed that the AgNPs biosynthesized from M. pulegium extract under alkaline conditions would act as better anticancer agents in biomedicine.

Evaluation of Antimicrobial Potential and Comparison of HPLC Composition, Secondary Metabolites Count, and Antioxidant Activity of Mentha rotundifolia and Mentha pulegium Extracts.

In the present study, the relationship between the phenolic counts, chemical composition, and biological activities of two Mentha species (Mentha rotundifolia (MR) and Mentha pulegium (MP)) was analyzed. The characterization of the action mode against pathogenic bacteria and the inhibition of spore germination of two fungal species using prepared methanolic extracts were studied here for the first time. The obtained data highlighted the presence of positive correlation between the secondary metabolites contents and the biological activities of the investigated extracts. In fact, HPLC analysis showed that the major components in both the extracts were eriocitrin and rosmarinic acid (25 and 20 mg/ml and 12 and 8 mg/ml in methanolic extracts of MR and MP, respectively). Moreover, the MR extract was rich in polyphenols and presents the highest antioxidant activity than MP ones. In addition, both extracts possess an antimicrobial activity against four Gram-positive and five Gram-negative bacteria and one yeast species (Candida albicans) and were able to inhibit the spore germination of two fungi species (Aspergillus niger and Aspergillus flavus). But, the significant activity was observed in the presence of MR methanolic extract. The effect of time on cell integrity of E. coli and L. monocytogenes determined by time-kill and bacteriolysis assays showed that the MR extract had a rapid bacteriolytic effect compared to the MP extract, and their capacities were significant against Gram-negative bacteria than positive ones. Based on the obtained data, it can be concluded that Saudi Mentha species have high pharmacological and industrial importance and they can be used in preparation of food or drugs.

Photoprotective, antioxidant, anticholinesterase activities and phenolic contents of different Algerian Mentha pulegium extracts

In this study, extracts of chloroform, ethyl acetate, n-butanol, and water were prepared from leaves of Mentha pulegium L. To assess their potential application as a source of bioactive compounds, the different extracts have been studied for their polyphenol content and for certain biological properties that might confer. Total phenolic (TPC) and total flavonoid (TFC) contents were estimated using colorimetric methods. 1,1-diphenyl-2-picrylhydrazyl (DPPH), [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] (ABTS), galvinoxyl radical (GOR), Cupric reducing antioxidant capacity (CUPRAC), reducing power, β-carotene bleaching, and O-phenanthroline assays were used to evaluate the antioxidant activity of the extracts. The inhibitory capacities of the extracts against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), as well as their photoprotective effect, were determined. The highest TPCs and TFCs were recorded in the extract of n-butanol (451.22 ± 1.54 μg GAE/mg of extract and 268.88 ± 2.65 μg QE/mg of extract, respectively), which had the highest antioxidant activity followed by ethyl acetate and water extracts. Most of the extracts studied were found to be inactive against AChE and BChE and only the chloroform extract showed a moderate inhibitory effect with percentages of inhibition of 13.16 ± 1.64 and 24.34 ± 1.12%, respectively at 200 μg/mL. High values of sun protection factor (SPF) were recorded with the extracts ranging from 33.86 ± 0.25 to 36.31 ± 0.40 corresponding respectively to the chloroform and aqueous extracts. The results of this work have shown that the phenolic extracts of Mentha pulegium can constitute a good choice for their application as antioxidant and photoprotective phytoconstituents.

Wound-healing activity of Algerian Lavandula stoechas and Mentha pulegium extracts: from traditional use to scientific validation

The aim of the present study has been to evaluate the antioxidant, antimicrobial and wound healing activity of Algerian Lavandula stoechas and Mentha pulegium methanol extracts (LE and ME, respectively) in order to scientifically validate their traditional use. After a preliminary phytochemical screening, a polyphenol profile characterization was carried out by RP-LC-DAD-ESI-MS/MS analysis. Antioxidant activity was evaluated by different in vitro assays such as DPPH, TEAC, FRAP and ORAC whereas antimicrobial activity was carried out against representative standard ATCC and clinical Gram-positive and Gram-negative strains as well as against Candida albicans. The wound healing activity of ointments containing 5% and 10% of LE and ME was investigated on Wistar albino rats. Different polyphenol classess (flavones, flavonols and phenolic acids) were identified with rosmarinic acid that was the most abundant compound in both extracts (5658.40 and 1201.35 mg/100 g of LE and ME, respectively). LE and ME have strong antioxidant activity, whereas they showed antimicrobial activity only against S. aureus. However, both 5% and 10% LE and ME ointments significantly accelerated the wound healing process in comparison with negative control, providing a scientific rationale for the traditional use of L. stoechas and M. pulegium aerial parts in the treatment of wounds.

In vitro activities of Traganum nudatum and Mentha pulegium extracts combined with amphotericin B against Candida albicans in production of hydrolytic enzymes.

Background and Purpose : Candida albicans is an important microorganism in the normal flora of a healthy subject; however, it has an expedient pathogenic character that induces hydrolytic virulence. Regarding this, the present study aimed to find an in vitro alternative that could reduce the virulence of this yeast.Materials and Methods: For the purpose of the study, the effect of amphotericin B (AmB) combined with the extract of Traganum nudatum (E1) or Mentha pulegium (E2) was evaluated against the hydrolytic activities of esterase, protease, and phospholipase. This effect was determined by calculating the minimum inhibitory concentration (MIC), used to adjust the extract/AmB mixtures in culture media.Results: The evaluated Pz values, which corresponded to the different enzymatic activities, showed a decrease in the hydrolytic activities of C. albicans strains after the addition of E1/AmB and E2/AmB combinations at descending concentrations (lower than the obtained MICs).Conclusion: Based on the findings, it would be possible to reduce the pathogenesis of this species without destabilizing the balance of the flora.

Hydro-Ethanolic Extract of Mentha pulegium Exhibit Anthelmintic and Antioxidant Proprieties In Vitro and In Vivo.

During recent decades, the emergence of chemoresistance among synthetic anthelmintic drugs has increased the interest in screening novel natural anthelmintic compounds derived from plants. The current study is aimed to determine the chemical profile, anthelmintic and antioxidant properties of Mentha pulegium hydro-ethanolic extract. Two tests were used to assess the in vitro anthelmintic activity of the hydro-ethanolic extract of M. pulegium against Haemonchus contortus; egg hatch assay (EHA) and adult worm motility (AWM) assay. M. pulegium extracts at the doses of 500, 1000, 2000 and 4000 mg/kg were evaluated in vivo in mice infected with Heligmosomoides polygyrus. The anthelmintic efficacy was monitored using faecal egg count reduction (FECR) and total worm count reduction (TWCR). The antioxidant activity of M. pulegium extract was evaluated by testing the total antioxidant capacity and the DPPH free radical-scavenging ability. Chromatographic characterization of M. pulegium composition using RP-HPLC revealed the presence of phenolic acids such as syringic acid, ferulic acid and the presence of flavonoid compounds, such as isorhamnetin-3-O-glucoside and kaempferol-3-O-rutinoside. We observed 91.58% inhibition in the EHA at 8 mg/mL after 48 h of incubation (IC50=1.82 mg/mL). In the AWM assay, M. pulegium extract achieved 65.2% inhibition at 8 mg/mL after 8 h. The highest dose (4000 mg/kg) showed a significant nematicidal effect 7 days post-treatment by inducing 60.39% FECR and 71.6% TWCR. We also report strong in vivo antioxidant capacity of the extract, as revealed by a significant increase of the enzymatic activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzymes in mice infected with H. polygyrus. Together, the results in this paper suggest that M. pulegium possesses anthelmintic properties and could be a potential source of novel compounds for the control of helminth parasites as well as its associated oxidative damage.

The Effect of Mentha Pulegium on Healing of Burn Wound Injuries in Rat

BACKGROUNDBurn is one of the most common injuries and the 4th common cause of trauma globally. This study was designed to evaluate the effect of the Mentha pulegium extract on second degree burn injuries in rats assessing histopathologic and macroscopic.METHODS M. Pulegium extract dressings was used as a treatment to deep dermal contact burns in rats, compared with two control groups of vaseline dressing and simple dressing by normal saline. After creating second-degree burn on the dorsum of rats, the treatments were applied for 15 min in three groups. Wound dressings were performed on days 1, 3, 7, 14 and 21st and at the same time macroscopic assessment was performed using a digital camera and software processing of photos. Pathologic evaluation of skin specimens was undertaken on days 1, 3,7, 14 and 21st. Wound healing parameters such as epithelialization, angiogenesis, granulation tissue formation, inflammatory cells were compared between these 3 groups.RESULTSApplication of M. Pulegium extract on second degree burn wounds significantly decreased burn surface area and increased fibroblasts in comparison to simple dressing. There was not any statistically significant relationship between M. Pulegium extract treated group and vaseline treated or simple dressing groups on other wound healing parameters. CONCLUSIONThis study delineated that M. Pulegium extract had a positive effect on healing process of second degree burns.

The Effect of Mentha Pulegium on Healing of Burn Wound Injuries in Rat

Burn is one of the most common injuries and the 4th common cause of trauma globally. This study was designed to evaluate the effect of the Mentha pulegium extract on second degree burn injuries in rats assessing histopathologic and macroscopic.M. Pulegium extract dressings was used as a treatment to deep dermal contact burns in rats, compared with two control groups of vaseline dressing and simple dressing by normal saline. After creating second-degree burn on the dorsum of rats, the treatments were applied for 15 min in three groups. Wound dressings were performed on days 1, 3, 7, 14 and 21st and at the same time macroscopic assessment was performed using a digital camera and software processing of photos. Pathologic evaluation of skin specimens was undertaken on days 1, 3,7, 14 and 21st. Wound healing parameters such as epithelialization, angiogenesis, granulation tissue formation, inflammatory cells were compared between these 3 groups.Application of M. Pulegium extract on second degree burn wounds significantly decreased burn surface area and increased fibroblasts in comparison to simple dressing. There was not any statistically significant relationship between M. Pulegium extract treated group and vaseline treated or simple dressing groups on other wound healing parameters.This study delineated that M. Pulegium extract had a positive effect on healing process of second degree burns.

Antimicrobial Effects of Mentha Pulegium Extract against Staphyloccocus Aureus Bacteria

The effects of aqueous and alcoholic of extracts Mentha pulegium leaves were studied on the growth of strain Staphylococcus aureus that isolated from burns. The Phytochemical analysis of The aqueous and alcoholic extracts of Mentha pulegium leaves show the presence of bioactive constituents like comarins, steroids, tannins, glycosides, saponins, alkaloids, flavonoids, phenols and essential oil. the ethanolic extract show an inhibition zone larger than aqueous extract.Results show that the ethanolic extract inhibits bacterial growth with inhibition zone(30.33mm) but the aqueous extract give an inhibition zone(8mm). In this study, minimum inhibitory concentration (MIC) was detected and the result varies according to the type of extract.

Mentha pulegium crude extracts induce thiol oxidation and potentiate hemolysis when associated to t-butyl hydroperoxide in human's erythrocytes.

Mentha pulegium (Lamiaceae) tea has been used as a traditional medicine; however, the modulatory effect of M. pulegium extracts on damage to human erythrocytes associated to t-butyl hydroperoxide (t-BHP) exposure remains to be investigated. Accordingly, we perform this study in order to test the hypothesis that aqueous and ethanolic extracts of M. pulegium could modulate the hemolysis associated to t-BHP exposure, non-protein thiol (NPSH) oxidation and lipid peroxidation (measured as thiobarbituric acid reactive substances - TBARS) in human erythrocytes. Samples were co-incubated with t-BHP (4 mmol/L) and/or aqueous or ethanolic extracts (10-1000 mg/mL) during 120 min to further analysis. We found that both extracts, when associated to t-BHP, potentiate NPSH oxidation and hemolysis. Moreover, both extracts significantly prevents against t-BHP-induced TBARS production. A significant correlation among hemolysis and NPSH levels was found. Taking together, our data points that the association of M. pulegium extracts with t-BHP culminates in toxic effect to exposed erythrocytes, besides its protective effect against t-BHP-induced TBARS production. So, we infer that the use of this extract may exert negative effect during painful crisis in sickle cell anemia. However, more studies are still necessary to better investigate/understand the mechanism(s) involved in the toxic effect resultant from this association.

Efficacy of Mentha pulegium extract in the treatment of functional dyspepsia: A randomized double-blind placebo-controlled clinical trial

Ethnopharmacological evidenceMentha pulegium L. leaves are used in the Iranian traditional medicine for the treatment of functional dyspepsia. Aim of study: To study the efficacy and safety of M. pulegium in the treatment of functional dyspepsia patients fulfilling the Rome III criteria. Materials and methodsThe efficacy and safety of a standardized Mentha pulegium leaf extract (drug extract ratio: 15.9:1, extraction solvent: 70% v/v aqueous ethanol) (330mg three times daily taken for 2 months) as add-on to one famotidine 40mg tablet per day in the treatment of 50 functional dyspepsia patients were compared with those of a parallel placebo group (n =50). ResultsThe extract significantly decreased the total dyspepsia score measured by the Hong Kong dyspepsia index compared to the placebo and baseline (P=0.011 and P<0.001 respectively). The stomach pain, upper abdominal bloating, upper abdominal dull ache, belching and total dyspepsia scores were decreased from baseline in the extract group significantly compared to the placebo (P<0.001, P<0.001, P=0.003, P<0.001 and P<0.001 respectively). However, the decreases of other dyspepsia symptoms scores from baseline in the extract group were not significant compared to the placebo (P>0.05). The extract improved the quality of life measured by the SF-36 questionnaire significantly compared to the placebo and baseline (P=0.003 and P<0.001 respectively). Moreover, the extract lowered the rate of H. pylori infection determined by the urease test significantly compared to the placebo and baseline (P=0.001 and P<0.001 respectively). The extract did not significantly affect the complete blood count and liver and kidney function tests (P>0.05). The patients did not experience any adverse drug effect. ConclusionsM. pulegium extract (genuine drug extract ratio: 19.4:1; extraction solvent: 70% v/v aqueous ethanol) 270mg three times daily taken for 2 months as adjunct to one famotidine 40mg tablet per day seems safe, improves dyspeptic symptoms and quality of life and eradicates H. pylori in functional dyspepsia patients.

An Investigation of Compositions and Effects of Local Herbal Glycyrrhiza glabra and Mentha pulegium extracts on Helicobacterpylori and Cell Line of stomach Cancer (AGS) by MTT assays

An Investigation of Compositions and Effects of Local Herbal Glycyrrhiza glabra and Mentha pulegium extracts on Helicobacterpylori and Cell Line of stomach Cancer (AGS) by MTT assays

Green synthesis of silver nanoparticles using Mentha pulegium and investigation of their antibacterial, antifungal and anticancer activity.

A simple and eco-friendly method for efficient synthesis of stable colloidal silver nanoparticles (AgNPs) using Mentha pulegium extracts is described. A series of reactions was conducted using different types and concentrations of plant extract as well as metal ions to optimize the reaction conditions. AgNPs were characterized by using UV-vis spectroscopy, transmission electron microscopy, atomic force microscopy, dynamic light scattering, zetasizer, energy-dispersive X-ray spectroscopy (EDAX) and Fourier transform infrared spectroscopy (FTIR). At the optimized conditions, plate shaped AgNPs with zeta potential value of -15.7 and plasmon absorption maximum at 450 nm were obtained using high concentration of aqueous extract. Efficient adsorption of organic compounds on the nanoparticles was confirmed by FTIR and EDAX. The biogenic AgNPs displayed promising antibacterial activity on Escherichia coli, Staphylococcus aureus, and Streptococcus pyogenes. The highest antibacterial activity of 25 µg mL-1 was obtained for all the strains using aqueous extract synthesized AgNPs. The aqueous extract synthesised AgNPs also showed considerable antifungal activity against fluconazole resistant Candida albicans. The cytotoxicity assay revealed considerable anticancer activity of AgNPs on HeLa and MCF-7 cancer cells. Overall results indicated high potential of M. pulegium extract to synthesis high quality AgNPs for biomedical applications.

LC-MS-based metabolite profiling of methanolic extracts from the medicinal and aromatic species Mentha pulegium and Origanum majorana.

There has been increasing interest dedicated to the phenolic compounds with a view to their antioxidant and healthy properties. Recent studies have focused on plants from the Lamiaceae family with special interest in phenolic compounds antioxidant potential. The metabolite profile of methanolic extracts from two Lamiacea medicinal plants was investigated. Mentha pulegium and Origanum majorana methanolic extracts were analysed using reversed-phase ultra-high-performance liquid chromatography (RP-UHPLC) coupled to electrospray ionisation quadrupole time-of-flight mass spectrometry (ESI-QTOF-MS) detection in the negative ion mode. A total of 85 metabolites were characterised from different families, such as organic acids and derivatives, amino acids and derivatives, nucleosides, phenolic compounds as well as other polar metabolites, by using the MS and MS/MS information provided by the QTOF-MS. However, the total phenols and flavonoids were also quantified spectrophotometrically and they registered higher amounts in Mentha pulegium than in Origanum majorana extract. Gallocatechin was the major compound in M. pulegium extract whereas quercetin dimethyl ether, jaceidin and dihydrokaempferide were the major ones in O. majorana extract. The distribution of phenolic compounds in the methanolic extract showed a variation among studied plants. Mentha pulegium can be considered as a source of gallocatechin.