Blue Sheep Research Articles

Prediction of potential habitat suitability of snow leopard (Panthera uncia) and blue sheep (Pseudois nayaur) and niche overlap in the parts of western Himalayan region

AbstractThe snow leopard (Panthera uncia) and blue sheep (Pseudois nayaur) are the inhabitants of remote areas at higher altitudes with extreme geographic and climatic conditions. The habitats of these least‐studied species are crucial for sustaining the Himalayan ecosystem. We employed the Maximum Entropy (MaxEnt) species distribution model to predict the potential habitat suitability of snow leopards and blue sheep and extracted common overlapped niches. For this, we utilised presence location, bio‐climatic and environmental variables, and correlation analysis was applied to reduce the negative impact of multicollinearity. A total of 134 presence locations of snow leopards and 64 for blue sheep were selected from the Global Biodiversity Information Facility (GBIF). The annual mean temperature (Bio1) was found to be the most useful and highly influential factor to predict the potential habitat suitability of snow leopards. Annual mean temperature, annual precipitation and isothermality were the major influencing factors for blue sheep habitat suitability. Highly influential bio‐climatic, topographic and environmental variables were integrated to construct the model for predicting habitat suitability. The area under the curve (AUC) values for snow leopard (0.87) and blue sheep (0.82) showed that the models are under good representation. Of the total area investigated, 47% was suitable for the blue sheep and 38% for the snow leopards. Spatial habitat assessment revealed that nearly 11% area from the predicted suitable habitat class of both species was spatially matched (overlapped), 48.6% area was unsuitable under niche overlap and 40.5% area was spatially mismatched niche. The presence of snow leopards and blue sheep in some highly suitable areas was not observed, yet such areas have the potential to sustain these elusive species. The other geographical regions interested in exploring habitat suitability may find the methodological framework adopted in this study useful for formulating an effective conservation policy and management strategy.

PRNP Sequences of Tibetan Antelope, Blue Sheep, and Plateau Pika from the Qinghai-Tibet Plateau and Reactivity of PrP Proteins to Rodent-Adapted Scrapie Strains in RT-QuIC and PMCA

Background: Tibetan antelope (Rhinopithecus), blue sheep (Pseudois nayauris), and plateau pika (Ochotona curzoniae) are wild animals living on the Qinghai-Tibet Plateau. There have been no reports of naturally-occurring transmissible spongioform encephalopathies (TSEs) involving these animals. Furthermore, the PRNP genes have not been described in the literature. Methods: The PRNP genes from 21 Tibetan antelopes, 4 blue sheep, and 3 plateau pikas were obtained and sequenced. The recombinant proteins were then prepared. Using scrapie strains (263K, 139A, ME7, and S15) as the seeds, the reactivity of the PrP proteins from sheep (rSheepPrP25-234) and pika (rPikaPrP23-230) were tested using real-time quaking-induced conversion (RT-QuIC). Protein misfolding cyclic amplification (PMCA) tests of the brain homogenates from domestic sheep and rabbits were performed with the seeds of strains 263K and ME7. Results: The PRNP genes of bovids were 771 bp long and encoded 256 amino acids (aa), showing 100% homology with the wild-type sheep prion protein (PrP) aa sequence. The PRNP gene of pika was 759 bp long and encoded 252 amino acids, showing 92.1% homology with the aa sequence of domestic rabbits. The sheep and pika proteins revealed positive reactions in 10-5 diluted seeds. Only rPikaPrP23-230 produced positive curves in 10-7 diluted seeds. The PMCA tests failed to produce proteinase K (PK)-resistant PrP (PrPres). Conclusion: This is the first description of PRNP genes and PrP aa sequences of Tibetan antelope, blue sheep, and plateau pike from the Qinghai-Tibet Plateau. In the presence of rodent prions, the PrPs of sheep and pika efficiently induce fibrillation in RT-QuIC, but do not generate PrPres in PMCA. Our results indicate that pika, as one of the important links in the Qinghai-Tibet Plateau biological chain, may play an important role in the prion circulation. Pika PrP deserves further analysis for its potential application value in assays for human prion disease.

基于最大熵模型的青海祁连山雪豹生境适宜性评价

PDF HTML阅读 XML下载 导出引用 引用提醒 基于最大熵模型的青海祁连山雪豹生境适宜性评价 DOI: 10.5846/stxb202009192440 作者: 作者单位: 作者简介: 通讯作者: 中图分类号: 基金项目: 国家重点研发项目课题(2017YFC0506405) Habitat suitability assessment of Panthera uncia in Qilian Mountains of Qinghai based on MAXENT modeling Author: Affiliation: Fund Project: National Key RESEARCH and Development Project (2017YFC0506405) : The second batch of Forestry Reform and Development Fund in 2018, the Rare and endangered wildlife protection and Assistance Project of Qilian Mountain National Park (Qinghai Region) 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:近年来,各种人为和自然因素导致了青海祁连山山区局部出现了严重的生态破坏,加剧了祁连山雪豹生境的退化,如何有效地对雪豹生境进行适宜性评价是恢复和改善祁连山生态环境的重要途径。本研究基于3S技术,在青海祁连山雪豹分布区利用红外相机监测、日常巡护监测以及雪豹粪便、脚印等痕迹获得其分布点数据并综合考虑气候、地形、植被、人为干扰等关键环境变量,运用MAXENT模型对青海祁连山区域雪豹生境适宜性进行分析评价。研究结果表明:岩羊密度、降雨和海拔是影响雪豹生境选择的关键因子,适宜生境总面积为13935.98 km2,占研究区域的37%,而大约48%的适宜生境分布在保护区外。现有功能区下雪豹保护的关键区域为位于保护区中部及西北部区域的祁连山中段疏勒南山-托勒南山区域,另有部分最适宜区位于保护区管辖范围之外的祁连县北部的沙龙滩管护站、托勒河源管护站及瓦乎斯管护站所在区域。 Abstract:Severely ecological damage in the Qilian Mountains is aggravating the fragmentation and degradation of the vulnerable snow leopard's habitat. This damage in recent years comes from both human and natural factors. Evaluating this habitat is a key component to improve, and perhaps restore, the creature's home. The data from infrared cameras, snow leopard excrement, and snow leopard footprints was integrated with climate, terrain, and disturbance factors. The MAXENT model was used to evaluate the habitat's suitability for the snow leopard. The results show that the rainfall, altitude, and the density of bharal (blue sheep) are critical factors for the habitat's suitability. About 37% (~14000 km2) of the study area was found to be suitable. Unfortunately, not all this suitable area was protected by the nature reserve. Snow Leopard protection is most critically needed in the Shulenan Mountain and Tuolenan Mountain areas within in the Qilian Mountain range, located in the central and northwestern region of the reserve.Outside of the reserve, Sharon Beach Management Station, Tuole River Headwater Management Station, and Wahusi Management and Protection Station in the northern part of Qilian County are also critical areas needing protection for the Snow Leopard. 参考文献 相似文献 引证文献

Anti-predator strategies of blue sheep (naur) under varied predator compositions: a comparison of snow leopard-inhabited valleys with and without wolves in Nepal

Context In Nepal, naur are usually the staple wild prey for the snow leopard, a solitary stalker hunter, and in some cases, for the wolf who hunts in a pack. We assumed that naur would adapt their anti-predatory responses to the presence of chasing and ambushing predators in the Manang Valley, where there are snow leopards and wolves, and in the Nar Phu valley, an area where there is only the snow leopard. Aims The aim of this study was to determine if there were differences in anti-predator strategies (vigilance, habitat selection and escape terrain) of naur in two valleys over two seasons, spring and autumn. Methods In spring 2019, we conducted a reconnaissance survey on the status of the naur and its habitat in the Manang and Nar Phu valleys of the Annapurna Conservation Area, Nepal. In spring and autumn 2020 and 2021, we observed 360 focal naur individuals (180 individuals in each valley), using the vigilance behaviour methodology to examine the behaviour of the naur. Key results There was little difference in the size of the naur groups between the Manang and Nar Phu valleys. The naur were twice as vigilant in Manang (15%), where there are snow leopards and wolves, as they were in Nar Phu (9%), with only snow leopards. The distance from the naur to escape cover was significantly shorter in Manang than in Nar Phu valley. Naur used significantly more rolling terrain in Nar Phu than in Manang. Conclusions The return of wolves to the Manang valley may have resulted in an increase in the level of naur vigilance. Most likely, the wolves in Manang have already had an effect on the female-to-young-ratio, and this effect will possibly have important consequences for the naur population, as well as at the ecosystem level in the future. Other key determining factors, such as the climate crisis and changes in local resources, could have a significant impact on the naur population, indicating the need for more research. Implications The findings of this study would provide valuable baseline information for the design of a science-based conservation strategy for conservation managers and scientists on naur, snow leopards and wolves.

Influences of Climate Change and Land Use Change on the Habitat Suitability of Bharal in the Sanjiangyuan District, China.

One of the biggest dangers to the degradation of biodiversity worldwide is climate change. Its oscillations in the future could result in potential alterations to species populations and habitat structure. With Sanjiangyuan District as the study site, an uncrewed aerial vehicle (UAV) was utilized to investigate the number and location of the bharal (Pseudois nayaur). The Maximum Entropy model and the Minimum Cumulative Resistance model (MaxEnt-MCR) were coupled to simulate the distribution of wildlife. On this basis, the future geographical distribution of bharal under different climate scenarios was simulated, and the ecological corridor and habitat centroid of bharal were revealed. The results showed that the suitable area of the bharal habitat was 4669 km2, which was mainly concentrated in the Maduo, Qumalai, and Gonghe counties. The potential distribution of the species under different future climate scenarios had a decreasing trend. Under the SSP-245 scenario, the habitat area of bharal in 2030 and 2050 decreased by 25.68 and 44.61% compared with the present situation and cumulatively decreased by 1199 and 2083 km2, respectively. Under the SSP-585 scenario, the habitat area of bharal in 2030 and 2050 decreased by 27.5 and 48.44%, with a total reduction of 1284 and 2262 km2, respectively. Furthermore, a complete loss of habitat was predicted in Gonghe County by 2050. In addition, it was observed that the landscape structure in Sanjiangyuan District would be more fragmented and complex. The continued climate change will seriously affect the habitat distribution of this species. Therefore, preventive measures, such as protecting habitat areas and establishing ecological corridors for bharal, should be implemented in the Sanjiangyuan District. Such measures should not focus solely on the potential degradation but should also be extended to include potential distribution areas for future migration.

Wildlife conservation in Myanmar: trade in wild sheep and goats for meat, medicine, and trophies, with links to China, India, and Thailand

In Myanmar, the hunting and trade of wildlife are increasingly recognised as a major threat to the persistence of species. We here focus on the trade and conservation of wild sheep and goats (Caprinae; Antilopinae) as these species are indeed hunted and traded for a variety of reasons. Seizure reports from 2000 to 2020 and 20 visits to four wildlife markets between 1998 and 2017 resulted in records of ~ 2,000 body parts, the equivalent of ~ 1,200 wild sheep and goats. When combined with data from previous surveys conducted over the same period, the number of wild sheep and goats recorded in trade increase substantially, i.e. serow (the equivalent of 1,243 animals), goral (213 animals), takin (190 animals), blue sheep (37 animals), and Tibetan antelope (10 animals). With records from 10 out of 15 States, trade appears to be widespread and persistent over time. There was poor concordance between seizure data and trade observations, but data from various surveys are largely in agreement. The most prevalent body parts in trade were horns, followed by plates (the frontal portion of the skull with horns still attached) and heads of freshly killed animals. These parts are offered for sale both for decorations and for their purported medicinal properties. Meat, fat, and rendered oils were observed frequently but because of mixture with other wildlife, it was challenging to confirm species identify or to convert this to number of animal equivalents. Tongues and eyes were offered for sale as medicine. In order to better protect wild sheep and goats in Myanmar, it is imperative that the illegal trade in their parts is more effectively curbed than at present. This is the responsibility of both the Myanmar authorities and, given the high prevalence of trade in border towns, their international partners, including China and Thailand.

Potential impact of trophy hunting on vigilance and flight behaviour in Blue Sheep (Bharal: Pseudois nayaur)

Conservation management is often integrated into a broader tourism context, and under some conditions allows wildlife trophy hunting to support its goals. In such cases it is generally acknowledged that the direct impact of hunting requires careful monitoring and regulation with respect to the size and dynamics of hunted populations. However, hunting may also affect the behaviour of local wildlife, including their reaction to the approach of humans. Thus, hunting may have broader consequences on tourism and conservation management if animals respond by changing their behaviour in a way that makes them more difficult to monitor or for tourists to observe. We examined the potential impact of trophy hunting on vigilance and flight behaviour of Blue Sheep (Bharal: Pseudois nayaur) in Nepal, by comparing their behavioural responses in conservation areas with contrasting management approaches: the Dhorpatan Hunting Reserve (DHR) where male Blue Sheep have been trophy hunted since the 1980 s, and the Annapurna Conservation Area (ACA) where hunting is forbidden. Blue Sheep in the DHR had higher levels of vigilance than sheep in the ACA (10 % versus 8 % of their time respectively). Sheep in the DHR were also much more difficult to approach on foot, with Blue Sheep groups in the DHR having an average flight initiation distance of 96 ± 7 m versus 39 ± 3 m for the ACA, and subsequently moving much greater distances when disturbed (flight movement distance in the DHR versus ACA: 79 ± 3 m versus 26 ± 2 m respectively). These results suggest that hunting impacts on tourism and conservation may extend well beyond the population dynamic consequences of trophy animal removal. These behavioural effects suggest additional consideration is required when balancing wildlife hunting and observation tourism activities in the same area. It would also be valuable to assess the impacts of hunting-induced behaviour changes on the effectiveness of wildlife monitoring in such areas.

Mitochondrial cytochrome b indicates the presence of two paraphyletic diverged lineages of the blue sheep Pseudois nayaur across the Indian Himalaya: conservation implications.

Populations exhibit signatures of local adaptive traits due to spatial and environmental heterogeneity resulting in microevolution. The blue sheep is widely distributed across the high Asian mountains and are the snow leopard's principal prey species. These mountains differ in their evolutionary history due to differential glaciation and deglaciation periods, orography, and rainfall patterns, and such factors causes diversification in species. Therefore, we assess the phylogeographic status of blue sheep using the mitochondrial cytochrome b gene (220bp) across the Indian Himalayan region (IHR) and its relationship with other populations. Of the observed five haplotypes, two and three were from the western Himalayas (WH) and eastern Himalayas (EH) respectively. One of the haplotypes from WH was shared with the population of Pamir plateau, suggesting historical maternal connectivity between these areas. The phylogenetic analyses split the blue sheep into two paraphyletic clades, and western and eastern populations of IHR were within the Pamir and Tibetan plateau clades, respectively. We observed a relatively higher mean sequence divergence in the EH population than in the WH. We propose five 'Evolutionary Significant Units' across the blue sheep distribution range based on observed variation in the species' ecological requirements, orography, climatic conditions, and maternal lineages, viz.; Western Himalaya-Pamir plateau (WHPP); Eastern Himalaya-Tibetan plateau (EHTP); Qilian mountains; Helan mountains and Hengduan mountains population. Despite the small sample size, population divergence was observed across the IHR, therefore, we suggest a transboundary, collaborative study on comparative morphology, anatomy, ecology, behaviour, and population genetics using harmonized different genetic markers for identifying the overall taxonomic status of the blue sheep across its range for planning effective conservation strategies.

Blue sheep strongly affect snow leopard relative abundance but not livestock depredation in the Annapurna Conservation Area, Nepal

Large carnivores play key roles in their ecosystems, but their protection is a major challenge in biodiversity conservation due to conflicts with human interests. The snow leopard ( Panthera uncia ) is the top predator of Asian high-altitude landscapes and faces various threats including wild prey depletion and illegal killings as a consequence of livestock depredation. As the interactions between snow leopards, wild prey, and livestock are still insufficiently understood, we studied the effects of 1) wild prey (blue sheep Pseudois nayaur and Himalayan marmots Marmota himalayana ) and domestic prey on snow leopard relative abundance, and of 2) these ecological parameters and intervention applications on livestock depredation by snow leopards. In the Annapurna Conservation Area, Nepal, we monitored wildlife populations and livestock along transects (490.8 km) in 82 grid cells (4 ×4 km) in 2019 and 2021 and conducted questionnaire surveys to determine livestock depredation between 2018 and 2021 (n = 479 households). We applied generalized linear models (GLMs) and sample comparison testing to examine the effects of prey densities and other environmental and anthropogenic predictors on snow leopard relative abundance and livestock depredation. Blue sheep density strongly positively affected snow leopard relative abundance, which also increased with terrain ruggedness and decreased with increasing densities of livestock and the human population. The size of livestock holdings shaped depredation events of large livestock (yak, cattle and horse), whereas depredation events of sheep and goats, which accounted for most (68.6%) depredated animals, decreased with increasing human population density and marmot presence. The strong impact of blue sheep on snow leopard relative abundance supports demands for integrating this ungulate into conservation and management plans, including wild prey recovery and translocation. The rather weak evidence for effects of blue sheep on depredation events suggests that conflicts over livestock depredation by snow leopards would neither be inflicted nor solved by increasing wild prey abundance. This demonstrates the need to improve intervention strategies in the Annapurna region, such as predator-proofing corrals and optimizing daytime herding practices. We suggest further exploring the effects of marmots and other secondary prey on livestock depredation rates, and testing the suitability of additional interventions, e.g., dogs and deterrents, as conflict mitigation tools. Our results will support wildlife managers in setting conservation priorities to promote the long-term co-existence of local people and snow leopards. • Blue sheep strongly shape snow leopard relative abundance in the Annapurna region. • Livestock losses in the Annapurna region are mainly associated with husbandry factors. • Wild prey restoration and improved husbandry practices should benefit snow leopards.

Molecular characterization and zoonotic potential of Enterocytozoon bieneusi in ruminants in northwest China

This study was conducted to examine the molecular characteristics and assess the zoonotic potential of Enterocytozoon bieneusi in ruminants in northwest China. A total of 1581 fresh fecal samples were collected from eight categories of ruminants. The E. bieneusi was screened and genotyped via nested polymerase chain reaction (PCR) amplification targeting the internal transcribed spacer (ITS) of the small subunit rRNA (ssu rRNA) gene. The result indicated that the average infection rate of E. bieneusi in ruminants was 16.0% (253/1581), with infection rates of E. bieneusi in Mongolian sheep, Mongolian goats, Chifeng cattle, red deer, alpine musk deer, and blue sheep at 21.8% (169/777), 8.2% (46/561), 25.9% (28/108), 13.2% (5/38), 20.0% (4/20), and 6.3% (1/16), respectively. The infections of E. bieneusi varied by different categories. For the different age groups, the infection rates in lambs (29.3%, 108/369) and calves (57.1%, 8/14) were significantly higher than that in ewes (21.1%, 215/1020) and cows (21.3%, 20/94). For the molecular characterization, diverse E. bieneusi ITS genotypes were identified, with a total of 13 genotypes were observed, including 10 known genotypes (BEB6, COS-I, J, CHC8, I, CHG1, BEB4, CHG3, CHS7, and NCF2) and 3 novel genotypes (CNR1 to CNR3). Genotype BEB6 was predominant (59.7%, 151/253). Phylogenetic analysis revealed that most E. bieneusi ITS genotypes clustered into group 2 and only one (NCF2) genotype belonged to group 1. The zoonotic genotypes identified in ruminants in the present study indicated the zoonotic potential of E. bieneusi. In addition, simultaneous identification of genotypes, such as BEB6, COS-I, and BEB4, in the same eco-geographical system indicated some host multiplicity transmission potential of E. bieneusi.

Coexistence mechanisms of sympatric ungulates: Behavioral and physiological adaptations of blue sheep (Pseudois nayaur) and red deer (Cervus elaphus alxaicus) in Helan Mountains, China

Studying the coexistence mechanisms of sympatric wildlife helps to shed light on why the earth has so many different species. When ungulates share ranges, food and habitat requirements may partially or fully overlap. Therefore, the aim of this study was to determine how sympatric ungulates share limited resources. Carcasses of 27 adult blue sheep (Pseudois nayaur) and three adult red deer (Cervus elaphus alxaicus) were collected in the Helan Mountains, China. Nutritive indices of plant species foraged and morphometric measurements of the digestive system of the two sympatric ungulates were determined. In addition, 120 passive, infrared motion-triggered cameras recorded spatial overlap and temporal overlap between the two species. Camera trapping revealed relatively limited spatial overlap and significantly different activity rhythms between blue sheep and red deer. Differences were also observed in stomach weight, surface enlargement factor of the rumen, and intestine length between the two species. However, the combined relative weight of the stomach and intestine was not different between species. The low spatiotemporal overlap decreased opportunities for encounters between sympatric blue sheep and red deer, and significant differences in digestive systems allowed the two species to consume different plant species or different parts of the same species. Thus, the two sympatric ungulates coexist harmoniously in the Helan Mountains because of long-term evolutionary behavioral and physiological adaptations that eliminate negative effects on the survival of the other species.

Landscape use and co-occurrence pattern of snow leopard (Panthera uncia) and its prey species in the fragile ecosystem of Spiti Valley, Himachal Pradesh.

The snow leopard (Panthera uncia) plays a vital role in maintaining the integrity of the high mountain ecosystem by regulating prey populations and maintaining plant community structure. Therefore, it is necessary to understand the role of the snow leopard and its interaction with prey species. Further, elucidating landscape use and co-occurrence of snow leopard and its prey species can be used to assess the differential use of habitat, allowing them to coexist. We used camera trapping and sign survey to study the interactions of snow leopard and its prey species (Siberian Ibex- Capra sibrica and Blue sheep-Pseudois nayaur) in the Spiti valley Himachal Pradesh. Using the occupancy modelling, we examined whether these prey and predator species occur together more or less frequently than would be expected by chance. To understand this, we have used ten covariates considering the ecology of the studied species. Our results suggest habitat covariates, such as LULC16 (barren area), LULC10 (grassland), ASP (aspect), SLP (slope) and DW (distance to water), are important drivers of habitat use for the snow leopard as well as its prey species. Furthermore, we found that the snow leopard detection probability was high if the site was used by its prey species, i.e., ibex and blue sheep. Whereas, in the case of the prey species, the probability of detection was low when the predator (snow leopard) was present and detected. Besides this, our results suggested that both species were less likely to detect together than expected if they were independent (Snow leopard—Ibex, Delta = 0.29, and snow leopard—blue sheep, Delta = 0.28, both the values are <1, i.e., avoidance). Moreover, despite the predation pressure, the differential anti-predation habitat selection and restriction of temporal activities by the prey species when snow leopard is present allows them to co-exist. Therefore, considering the strong link between the habitat use by the snow leopard and its prey species, it is imperative to generate quantitative long-term data on predator-prey densities and the population dynamics of its prey species in the landscape.

Dietary diversity and niche partitioning of carnivores across the Qinghai–Tibetan Plateau of China using DNA metabarcoding

Abstract Understanding predator guild resource use is vital for carnivore species preservation and ecosystem function in high-altitude landscapes. We describe the dietary composition, similarity, and niche overlap for seven carnivore species across three regions of the Qinghai–Tibetan Plateau (Qilian Shan, East Burhanbuda Mountain, Yushu) using DNA metabarcoding of 760 scat samples. Analyses found 33 unique prey items representative of the classes Mammalia, Aves, and Actinopterygii. Blue sheep (Pseudois nayaur) and pika (Ochotona sp.) were most frequently detected. Livestock in carnivore diets ranged from 4% to 7% depending on site, but accounted for 21.7% of Tibetan wolf (Canis lupus) diet. Eurasian lynx (Lynx lynx), Tibetan fox (Vulpes ferrilata), Pallas’s cat (Otocolobus manul), and snow leopard (Panthera uncia) had highly uneven diets. Intraguild predation of mesocarnivores by apex carnivores was found. Analysis of dietary niche overlap using Pianka’s index showed that overlap was significantly greater than expected among all carnivores examined and between apex and mesocarnivores, though significant correlations between carnivore body mass and prey body mass suggested broad separation in consumed prey. Snow leopard and Tibetan wolf diets were consistently the most similar of any two given species pairs. This study provides evidence of high dietary overlap. Protection for species consumed by a wide variety of carnivores will benefit predator guilds in the area and assist in maintaining the relatively low frequency of livestock predation. This work provides insight into the dynamics of carnivore guilds living in an area that has worldwide impacts, and methods herein could be applied on global scales for conservation efforts.

Feeding Patterns of Three Widespread Carnivores—The Wolf, Snow Leopard, and Red Fox—in the Trans-Himalayan Landscape of India

The Trans-Himalayan landscape is an extreme cold desert with limited diversity and a low density of wild prey. The landscape has three widespread carnivores—the wolfCanis lupus chanco, snow leopardPanthera uncia, and red foxVulpes vulpes—competing for similar prey. We studied predation patterns, dietary competition, and niche segregation of three sympatric carnivores in the Indian Trans-Himalayas. The Himalayan or wooly wolf is one of the oldest lineages and has adapted to extreme cold. We further studied the prey selection of the wolf in the high-altitude environment. We collected 1,600 carnivore scats (wolf = 542, snow leopard = 31, and red fox = 1,027), and additional data on 573 snow leopard scats from literature was used in the analyses. We found that livestock was a major contributor to the wolf (56.46%) and snow leopard diet (30.01%). The wolf consumed blue sheep (8.26%) and ibex (3.13%), whereas the snow leopard subsisted on blue sheep (30.79%) and ibex (17.15%), relatively more frequently. The red fox preyed upon small species like pika and marmots (18.85 %); however, livestock carrions (16.49%), fruits and seeds (15.05%), and human-derived material (11.89%) were also consumed frequently. The dietary niche of three carnivore species highly overlapped (Pianka's index = 0.503, simulated mean = 0.419,p= 0.15) due to livestock. The carnivores segregated their diet in prey items originating from the wild. We tested a relationship between carnivore diet variations and prey/predator functional traits using RLQ ordination. Predator morphology traits like body size, weight, and habitat preference significantly affected the prey selection (p&amp;lt; 0.05). The wolf and snow leopard avoided the competition through habitat selection, while the red fox coexisted by exploiting a broader niche. The wolf showed site-specific variation in prey preferences albeit no prey selection at the landscape level (G2 = 6.79,df= 12,p= 0.87). The wolf preferred wild prey over domestic species at the wild prey rich site. The carnivores in this pastoralist landscape have adapted to exploit livestock resources despite facing persecution. Therefore, managing livestock and simultaneously restoring wild prey is crucial for the conservation of the carnivore guild in the Trans-Himalayan ecosystem.

Spatial separation of prey from livestock facilitates coexistence of a specialized large carnivore with human land use

AbstractThere is an increasing emphasis in conservation strategies for large carnivores on facilitating their coexistence with humans. Justification for coexistence strategies should be based on a quantitative assessment of currently remaining large carnivores in human‐dominated landscapes. An essential part of a carnivore's coexistence strategy has to rely on its prey. In this research, we studied snow leopards Panthera uncia whose habitat mainly comprises human‐dominated, unprotected areas, to understand how a large carnivore and its primary prey, the bharal Pseudois nayaur, could coexist with human land use activities in a large proportion of its range. Using a combination of livestock census, camera trapping and wildlife surveys, across a broad gradient of livestock grazing intensity in a 363 000 km2 landscape on the Tibetan Plateau, we found no evidence of livestock grazing impacts on snow leopard habitat use, bharal density and spatial distribution, even though livestock density was 13 times higher than bharal density. Bharal were found to prefer utilizing more rugged habitats at higher elevations with lower grass forage conditions, whereas livestock dominated in flat valleys at lower elevations with higher productivity, especially during the resource‐scarce season. These findings suggest that the spatial niche separation between bharal and livestock, together with snow leopards' specialized bharal diet, minimized conflicts and allowed snow leopards and bharal to coexist in landscapes dominated by livestock grazing. In recent years, reduced hunting and nomadic herder's lifestyle changes towards permanent residence may have further reinforced this spatial separation. Our results indicated that, for developing conservation strategies for large carnivores, the niche of their prey in relation to human land‐use is a key variable that needs to be evaluated.

Effect of seasonal changes on the innate immunity of wild Pseudois nayaur: potential reason for its endangerment.

Seasonal climate change may impose stress on mammal's immune system, but this has never been connected to wildlife endangerment reasons. This study focuses on Pseudois nayaur, a rare Chinese sheep facing a severe situation of disappearing. Wild Pseudois nayaurs in Sichuan, China were observed in this research by an integrated method including habitat environment, foraging rules, and breeding activities investigation in ecology filed, accompanied with immunity research by peripheral blood, including erythrocyte, neutrophils, natural killer cells, and macrophages immunity by means of erythrocyte rosette, phagocytosis, lactate dehydrogenase release and acid alpha-naphthyl acetate esterase method, respectively. The results show that Pseudois nayaurs's innate and red blood cell immunity in autumn and winter Pseudois nayaurs and it may be the results of energy reallocation between reproduction and the immune system. In addition, the environment pressure found in Zhu Balong, Sichuan, China including food reduction, human disturbance, low temperature, and severe drought still existed, which may prevent the Pseudois nayaur from normal immunity enhancement before winter. In conclusion, the innate immunosuppression found in this study especially at their breeding season in autumn and winter may be the ignored cause of wild Pseudois nayaur's endangerment and should be considered in their protection strategy.

Expanding or shrinking? range shifts in wild ungulates under climate change in Pamir-Karakoram mountains, Pakistan.

Climate change is expected to impact a large number of organisms in many ecosystems, including several threatened mammals. A better understanding of climate impacts on species can make conservation efforts more effective. The Himalayan ibex (Capra ibex sibirica) and blue sheep (Pseudois nayaur) are economically important wild ungulates in northern Pakistan because they are sought-after hunting trophies. However, both species are threatened due to several human-induced factors, and these factors are expected to aggravate under changing climate in the High Himalayas. In this study, we investigated populations of ibex and blue sheep in the Pamir-Karakoram mountains in order to (i) update and validate their geographical distributions through empirical data; (ii) understand range shifts under climate change scenarios; and (iii) predict future habitats to aid long-term conservation planning. Presence records of target species were collected through camera trapping and sightings in the field. We constructed Maximum Entropy (MaxEnt) model on presence record and six key climatic variables to predict the current and future distributions of ibex and blue sheep. Two representative concentration pathways (4.5 and 8.5) and two-time projections (2050 and 2070) were used for future range predictions. Our results indicated that ca. 37% and 9% of the total study area (Gilgit-Baltistan) was suitable under current climatic conditions for Himalayan ibex and blue sheep, respectively. Annual mean precipitation was a key determinant of suitable habitat for both ungulate species. Under changing climate scenarios, both species will lose a significant part of their habitats, particularly in the Himalayan and Hindu Kush ranges. The Pamir-Karakoram ranges will serve as climate refugia for both species. This area shall remain focus of future conservation efforts to protect Pakistan's mountain ungulates.

Diet of Snow Leopard Panthera uncia in Kanchanjunga Conservation Area

The population of Snow Leopard is declining due to anthropogenic activities and prey scarcity. Snow Leopard’s preferred prey species are Tibetan Argali, Blue Sheep, Himalayan Tahr, Himalayan Marmot, Royle’s Pika, rodents, and livestock such as Yak, Horse, Lulu Cow, Goat and Sheep. Due to livestock depredation by Snow Leopards and other carnivores, local people have a negative attitude towards carnivores’ conservation leading to retaliatory killing. This study identified whether Snow Leopards prey on wildlife or livestock based on micro-histological study of Snow Leopard’s scats. Micro-histological analysis of 39 scat samples of Snow Leopard confirmed that Blue Sheep is the major prey species in Kanchanjunga Conservation Area (KCA). The scat analysis also showed that Snow Leopards prey on livestock mainly to Chauri in KCA, however the number and incidences were few. The herders also confirmed the occasional hunting of their livestock depredation by Snow Leopard in KCA. People have positive attitudes towards Snow Leopard conservation because they are less suffering from Snow Leopard in KCA. If wild prey species are abundant in the wild, livestock depredation might be low.

A Glimpse of Blue Sheep (Pseudois nayaur) Hunting in Api Nampa Conservation Area (ANCA) Nepal

Higher altitudes of Api Nampa Conservation Area have become a hunting destination for the local people bringing a threat to blue sheep and other wildlife. Blue sheep has been a national concern in Nepal due to being the main prey species of snow leopard and trophy hunting. The purpose of this paper is to examine the impact of hunters in Api Nampa Conservation Area (ANCA), Darchula, Nepal. This study was carried out around Api Nampa Conservation Area in 2016. Mixed methods of questionnaires were developed to collect data, and quantitative analysis was done by applying SPSS 16. A hundred percent of hunters like to hunt in this protected area. The hunted animals were goral, blue sheep, barking deer, boar and tahr. Site-specific characteristics like vegetation density, terrain and rugged land topography often vary markedly across surveys. Due to the regular and yearly use of grazing by the livestock in the area, it becomes imperative to study their feeding habits and document their food plants. Consequently, poaching and livestock grazing are important challenges for the conservation of blue sheep. Hunting, deforestation, forest fires, grazing and shifting agriculture are identified as major threats to the wildlife of Api Nampa Conservation Area. Envisioning, critical thinking, building partnership in decision-making, awareness, and stringent action against hunting activities must be adopted early. Api Nampa Conservation Area has received little research attention from grassland ecologists and specialists in pastoral management.

The Discontinuous Elevational Distribution of an Ungulate at the Regional Scale: Implications for Speciation and Conservation.

Simple SummaryThe Himalaya blue sheep (Pseudois nayaur) in Baima Snow Mountain is found to exhibit a distinctive bimodal distribution along elevation gradient contrasting the unimodal distributions of most species. The first distributional peak of Himalaya blue sheep was in scree habitat around 4100 m a.s.l., while the second peak was in the dry-hot valley around 2600 m. Geographic separation from the original population along elevation suggested that population at lower elevation could be a separate species ecologically, or a taxa ongoing differentiation. Invasive species Opuntia ficus-indica, which colonized the region six hundred years ago, may have formed new foraging niche to support population at lower elevation. Our results suggested conservation measures should pay attention to taxa ongoing differentiation, and consider the possible active effect of biological invasion.The elevational range where montane species live is a key factor of spatial niche partitioning, because the limits of such ranges are influenced by interspecies interaction, abiotic stress, and dispersal barriers. At the regional scale, unimodal distributions of single species along the elevation gradient have often been reported, while discontinuous patterns, such as bimodal distributions, and potential ecological implications have been rarely discussed. Here, we used extensive camera trap records to reveal the elevation distribution of Himalaya blue sheep (Pseudois nayaur) and its co-existence with other ground animal communities along a slope of Baima Snow Mountain, southwest China. The results show that Himalaya blue sheep exhibited a distinctive bimodal distribution along the elevation gradient contrasting the unimodal distributions found for the other ungulates in Baima snow mountain. A first distributional peak was represented by a population habituating in scree habitat around 4100 m, and a second peak was found in the dry-hot valley around 2600 m. The two distinct populations co-existed with disparate animal communities and these assemblages were similar both in the dry and rainy seasons. The extremely low abundance of blue sheep observed in the densely forested belt at mid-elevation indicates that vegetation rather than temperature is responsible for such segregation. The low-elevation population relied highly on Opuntia ficus-indica, an invasive cactus species that colonized the region six hundred years ago, as food resource. Being the only animal that developed a strategy to feed on this spiky plant, we suggest invasive species may have formed new foraging niche to support blue sheep population in lower elevation hot-dry river valleys, resulting in the geographic separation from the original population and a potential morphological differentiation, as recorded. These findings emphasize the important conservation values of role of ecological functions to identify different taxa, and conservation values of apparent similar species of different ecological functions.