Protein Yield Research Articles

Nitrogen modulates the ozone response of Mediterranean wheat: Considerations for ozone risk assessment

The experiment was conducted in an Open Top Chamber facility located in the Mediterranean basin to investigate how nitrogen (N) fertilization affects the response of wheat to ozone (O3) exposure. The study considered the response of Artur Nick, a modern wheat cultivar commonly used in the area, to three O3 exposure levels (ambient and elevated ambient, +20 and +40 nL L−1 O3), and two N fertilization doses (100 and 200 kg ha−1). Measurements included leaf gas exchange, leaf chlorophyll content, leaf and grain N content, plant growth and yield parameters. Ozone × N interactive effects were studied and quantified based on accumulated O3 concentrations above a 40 nL L−1 threshold (AOT40) and phytotoxic O3 dose (POD) indices, which are used in O3-risk assessments, from which critical levels (CL) for a 5 % effect were derived.Results revealed that O3 impacts on growth and yield parameters were stronger under the highest N fertilization dose. In consequence, O3 Critical Levels (CL) were as much as 3–4 times lower for grain yield in the high-N compared to the low-N treatment. Interestingly, O3 limited the fertilizer stimulus, strongly reducing the N use efficiency for grain yield and the agronomic efficiency of N for protein yield. Another important aspect was that 71 % of the POD was accumulated before anthesis, stressing the potential importance of O3 exposure during the vegetative phase of wheat under Mediterranean conditions, which is usually considered less important than post-anthesis exposure.In conclusion, this study suggests the need to consider crop N management in the derivation of O3 CLs, due to its effect on dose-response relationships used for CL derivation, including the potential O3 effects in N use efficiency. Therefore, N modulation could be considered in the O3-risk assessment methodology to be applied in risk exercises when negotiating air pollution abatement policies.

The growth-promoting effect of water extract of Chuanminshen violaceum stem and leaf on broilers

Currently, developing non-antibiotic growth promoters is a broad consensus in broiler industry, which is one of the effective ways to reduce drug-resistant strains. Chuanminshen violaceum is a traditional Chinese medicinal herb that is commonly used for its roots, while the stems and leaves are often discarded, resulting in a huge amount of waste. This study optimized the preparation process of water extract of Chuanminshen violaceum stems and leaves (CVSLE) by response surface analysis based on the yields of polysaccharide and protein. The CVSLE and herbal powder (CVSL) were then processed into granules before being used as feed additives. The Macleaya cordata powder was used as positive control. The results showed that the addition of CVSLE (0.5% of the feed) showed the highest growth-promoting activity than other CVSLE groups (0.2% and 1%), 1% CVSL group and positive control (0.05%). CVSLE at the dosage of 0.5% could significantly increase the ADG and reduce the FCR from d 21 to 42, d 0 to 42. The HI antibody titers against Newcastle disease virus and avian influenza virus were significantly enhanced at 21, 28 and 42d. CVSLE did not affect the slaughtering performances, but could significantly elevate the spleen, thymus and bursa of Fabricius indices and the transcriptional levels of IL-2, IL-4, IL-10 and IFN-γ in spleen. The intestinal barrier function of broilers was significantly enhanced by increased levels of immune barrier (sIgA), physical barrier (ZO-1, OCL and Muc-2) and flora barrier (Lactobacillus and Bifidobacterium). These results suggest that CVSLE was a promising herbal additive candidate for broilers.

Examining performance, milk, and meat in ruminants fed with macroalgae and microalgae: A meta-analysis perspective.

This meta-analysis consolidates various related studies to identify patterns in the impact of feeding algae on performance aspects, including milk fat, milk protein, and carcass yield in several ruminant species, such as cattle, sheep, and goats. The data were collected from 67 articles that examined factors such as the type of algae (macro- and microalgae), algal species, and animal breed. Barki sheep, Moghani sheep, and Zaraibi goats demonstrated an increased average daily gain (P < 0.05) when fed with both macro- and microalgae. Conversely, sheep such as Canadian Arcott and Ile-de-France showed adverse effects on the feed conversion ratio (FCR) (P < 0.05). Elevated FCR values were observed across castrated and young animals (P < 0.05). Algae extract notably increased the hot carcass weight (P < 0.001), particularly among Moghani sheep (P < 0.001). Raw algae significantly reduced the milk fat content (P < 0.001), particularly in cattle and sheep (P < 0.001). A decrease in milk fat was particularly noticeable in lactating females of Assaf sheep, Damascus goats, and Holstein cows (P < 0.001). Overall, algae inclusion tended to decrease the milk protein content (P < 0.05), leading to reduced milk production (P < 0.001) with cumulative algae feeding in Assaf sheep. However, conjugated linoleic acid (CLA; C18:2 c9,t11-CLA and C18:2 c12,t10-CLA) and docosahexaenoic acid (DHA; C22:6n-3) mostly increased in meat and milk from Holstein cow, Assaf sheep, Dorset sheep, and Ile-de-France sheep (P < 0.01). This meta-analysis highlights the necessity for additional research aimed at optimizing the sustainable use of algae in feed for ruminants, despite the demonstrated improvements in performance and the levels of CLA and DHA found in meat and milk.

Differences in Lactation Performance, Rumen Microbiome, and Metabolome between Montbéliarde × Holstein and Holstein Cows under Heat Stress.

Heat stress negatively affects lactation performance and rumen microbiota of dairy cows, with different breeds showing varying levels of heat tolerance. This study aimed to compare the lactation performance of Montbéliarde × Holstein (MH, n = 13) and Holstein (H, n = 13) cows under heat stress, and 16S rRNA sequencing and liquid chromatography-mass spectrometry (LC-MS) were used to determine the rumen microbiome and metabolome in experimental cows. The results indicated that during heat stress, milk yield (p = 0.101), milk fat yield, milk protein yield, milk protein, and milk lactose (p < 0.05) in Montbéliarde × Holstein cows were higher than those in Holstein cows, whereas milk yield variation and somatic cell counts (p < 0.05) were lower than those in Holstein cows. The sequencing results indicated that the rumen of Montbéliarde × Holstein cows was significantly enriched with beneficial bacteria, such as Rikenellaceae, Allobaculum, and YRC22 (p < 0.05). In addition, correlations were observed between specific ruminal bacteria and lactation performance. Ruminal metabolites related to antioxidant and anti-inflammatory properties were significantly higher (p < 0.05) in Montbéliarde × Holstein cows than in Holstein cows. Overall, Montbéliarde × Holstein cows showed higher production efficiency under heat stress, which may be related to the different rumen mechanisms of crossbred and Holstein cows in adapting to heat stress.

Improved production of class I phosphatidylinositol 4,5-bisphosphate 3-kinase

Phosphatidylinositol 4,5-bisphosphate 3-kinases (PI3K) are a family of kinases whose activity affects pathways needed for basic cell functions. As a result, PI3K is one of the most mutated genes in all human cancers and serves as an ideal therapeutic target for cancer treatment. Expanding on work done by other groups we improved protein yield to produce stable and pure protein using a variety of modifications including improved solubility tag, novel expression modalities, and optimized purification protocol and buffer. By these means, we achieved a 40-fold increase in yield for p110α/p85α and a 3-fold increase in p110α. We also used these protocols to produce comparable constructs of the β and δ isoforms of PI3K. Increased yield enhanced the efficiency of our downstream high throughput drug discovery efforts on the PIK3 family of kinases.

Novel Sustainable Bio-fertilizer Formulated with Mangrove-associated Bacteria Enhances Duckweed Growth and Protein Content

Duckweed is a future food and a source of affordable protein that has the potential to replace animal protein. This study aims to formulate a bio-fertilizer consisting of mangrove-associated bacteria to boost the growth and protein of duckweeds as a sustainable approach to increase plant-based protein yields. The culture-depending technique was performed by using Aleksandrow agar, Pikovskaya’s agar, and Jensen agar to screen potassium-solubilizing bacteria, phosphate-solubilizing bacteria and nitrogen-fixing bacteria, respectively, from mangrove soil sediments. Mangrove-associated bacteria that are close to &lt;i&gt;Acinetobacter radioresistens&lt;/i&gt;, &lt;i&gt;Brachybacterium paraconglomeratum&lt;/i&gt;, and &lt;i&gt;Enterobacter cloacae&lt;/i&gt;, which are known as nitrogen-fixing bacteria, &lt;i&gt;Klebsiella quasipneumoniae&lt;/i&gt;, &lt;i&gt;Bacillus tropicus&lt;/i&gt;, and &lt;i&gt;Paenibacillus pasadenensis&lt;/i&gt; known as potassium-solubilizing bacteria, and &lt;i&gt;Bacillus cereus&lt;/i&gt; and &lt;i&gt;Bacillus thuringiensis&lt;/i&gt; known as phosphate-solubilizing bacteria were identified through 16S rRNA gene sequencing. After that, three sets of bio-fertilizers were randomly formulated. Each set consisted of nitrogen-fixing bacteria, potassium- and phosphate-solubilizing bacteria, as well as commercial compost as a carrier. These formulated bio-fertilizers were evaluated for plant growth promotion and protein production on duckweed plants under temperatures between 26 and 30°C. The results showed that each set of our formulated bio-fertilizer can increase the nitrogen (N), phosphorus (P), and potassium (K), duckweed growth, and protein content when compared to the control group. It indicates that bio-fertilizers formulated with mangrove-associated bacteria and high NPK contents could enhance the growth of duckweed as well as its protein content, which could supply our future plant-based protein sustainably.

Effect of Different Fertilizer and Silicon Levels on Grain Quality of Maize (Zea mays L.)

A field experiment with spilt plot design was conducted to study the ‘effect of fertility and silicon levels on the nutritional quality of maize’ during Kharif 2022 and 2023 at Rajasthan College of Agriculture (RCA), MPUAT, Udaipur, Rajasthan, India. The main plot treatments consist of four levels of Compounded NPK fertilizer (F0: Control, F50: 50% RDF, F75: 75% RDF and F100: 100 % RDF) and the sub-plot treatments consist of four levels of soil application of Diatomaceous Earth (Si0, Si100, Si200 and Si300). Pratap Makka-9 of maize variety was used for research purpose. The protein content, protein yield, carbohydrate and energy in maize grain were higher under 100% RDF than other levels of fertilizer. Application of 300 kg Diatomaceous Earth per hectare recorded higher protein content, protein yield, carbohydrate and energy in maize grain over the rest of silicon levels. The crude fat in maize grain was lower in 100% RDF. Application of 300 kg Diatomaceous Earth per hectare recorded lower crude fat in maize grain. Significantly higher protein yield was recorded under the combined application of 100% RDF and 300 kg diatomaceous earth per hectare. However, protein yield was at par in the application of 75% RDF with 300 kg Diatomaceous Earth per hectare and 100% RDF with 200 kg Diatomaceous Earth per hectare. The lowest crude fat was recorded under the combined application of 100% RDF and 300 kg Diatomaceous Earth per hectare over the rest of treatment combinations. Application of 100% RDF with 300 kg DE ha-1 (F100Si300) was recommended treatment for nutritional high quality maize grain.

Towards Self-regeneration: Exploring the Limits of Protein Synthesis in the Protein Synthesis Using Recombinant Elements (PURE) Cell-free Transcription-Translation System.

Self-regeneration is a key function of living systems that needs to be recapitulated in vitro to create a living synthetic cell. A major limiting factor for protein self-regeneration in the PURE cell-free transcription-translation system is its high protein concentration, which far exceeds the system's protein synthesis rate. Here, we were able to drastically reduce the nonribosomal PURE protein concentration up to 97.3% while increasing protein synthesis efficiency. Although crowding agents were not effective in the original PURE formulation, we found that in highly dilute PURE formulations, addition of 6% dextran considerably increased protein synthesis rate and total protein yield. These new PURE formulations will be useful for many cell-free synthetic biology applications, and we estimate that PURE can now support the complete self-regeneration of all 36 nonribosomal proteins, which is a critical step toward the development of a universal biochemical constructor and living synthetic cell.

Capillary mediated vitrification is a novel technique that enables storage of antibody critical reagents at ambient temperature: Impact on binding, structure, and laboratory sustainability

Antibodies and antibody conjugates are essential components of life science research, but their inherent instability necessitates cold storage or lyophilization, posing logistical and sustainability challenges. Capillary-mediated vitrification has shown promise as a tool for improving biomolecule stability. In this study, we assess the feasibility of shipping and storing CMV-stabilized antibody reagents at ambient temperature using a purified rabbit polyclonal as a model system. The conditions tested included a simulated temperature excursion, ambient shipping, and storage for approximately two months at room-temperature. Antibody function was measured by both ELISA and Octet bio-layer interferometry kinetic measurements. Yield, aggregation, and thermal stability were assessed by UV/VIS, Size Exclusion Chromatography (SEC), thermal melting, and thermal aggregation studies. Results indicate >97 % protein yield and no impact on the binding activity. No evidence of aggregation or oligomer formation was detected. Addition of the vitrification buffer to the sample matrix resulted in an increase in the aggregation on-set temperature, indicating enhanced thermostability. A slight shift in both the SEC retention time for the main peak and a difference in aggregation behavior at high temperatures were noted post-vitrification. We hypothesize that these differences are related to the interaction of the protein with the saccharide component of the vitrification matrix and the stabilization mechanism of sugars. The cumulative data supports the use of Capillary Mediated Vitrification as a viable alternative to frozen reagent storage, with the potential to significantly impact reagent stability, assay performance, laboratory operations, and sustainability initiatives.

Effect of Postbiotic Supplementation on Nutrient Digestibility and Milk Yield during the Transition Period in Dairy Cows.

The metabolites secreted by probiotics or released after their lysis are called postbiotics. They provide physiological benefits to the host, preventing the colonisation of pathogens by improving the intestinal environment for beneficial commensal bacteria, which reduces the incidence of digestive disorders and improves the immune system. The aim of this work was to evaluate the addition of postbiotics to dairy cow rations during the transition period on nutrient digestibility, composition, and milk yield. The effects of two postbiotics were evaluated in twelve Friesian cows from 30 days before calving to two months of lactation. The animals were randomly allocated to two treatment groups: control (CT) and supplemented with postbiotics (PC and PR). Feeding was ad libitum with 60/40 of forage/concentrate ratio on dry matter basis. Daily feed intake and milk production were recorded individually throughout the study. Two digestibility balances were performed, one before parturition and one after parturition. Colostrum was sampled at first milking and milk was sampled weekly. Data were analysed using a mixed mode in R software 4.4.1. The results suggest that postbiotic supplementation in late gestation and early lactation increase the voluntary intake of dry matter, especially in the PR treatment, with higher apparent total tract digestibility of dry matter, organic matter and neutral detergent fibre. Both treatments including postbiotics induced an increase in colostral immunoglobulin concentration. Milk production of cows receiving the PC treatment was the highest, with high fat and protein yields and a higher persistence of the production curve throughout the lactation.

Phenotypic and Genetic Analyses of Mastitis, Endometritis, and Ketosis on Milk Production and Reproduction Traits in Chinese Holstein Cattle.

Mastitis (MAS), endometritis (MET), and ketosis (KET) are prevalent diseases in dairy cows that result in substantial economic losses for the dairy farming industry. This study gathered 26,014 records of the health and sickness of dairy cows and 99,102 data of reproduction from 13 Holstein dairy farms in Central China; the milk protein and milk fat content from 56,640 milk samples, as well as the pedigree data of 37,836 dairy cows were obtained. The logistic regression method was used to analyze the variations in the prevalence rates of MAS, MET, and KET among various parities; the mixed linear model was used to examine the effects of the three diseases on milk production, milk quality, and reproductive traits. DMU software (version 5.2) utilized the DMUAI module in conjunction with the single-trait and two-trait animal model, as well as best linear unbiased prediction (BLUP), to estimate the genetic parameters for the three diseases, milk production, milk quality, and reproductive traits in dairy cows. The primary findings of the investigation comprised the following: (1) The prevalence rates of MAS, MET, and KET in dairy farms were 20.04%, 10.68%, and 7.33%, respectively. (2) MAS and MET had a substantial impact (p < 0.01) on milk production, resulting in significant decreases of 112 kg and 372 kg in 305-d Milk Yield (305-d MY), 4 kg and 12 kg in 305-d Protein Yield (305-d PY), and 6 kg and 16 kg in 305-d Fat Yield (305-d FY). As a result of their excessive 305-d MY, some cows were diagnosed with KET due to glucose metabolism disorder. The 305-d MY of cows with KET was significantly higher than that of healthy cows (205 kg, p < 0.01). (3) All three diseases resulted in an increase in the Interval from Calving to First Service (CTFS, 0.60-1.50 d), Interval from First Service to Conception (FSTC, 0.20-16.20 d), Calving Interval (CI, 4.00-7.00 d), and Number of Services (NUMS, 0.07-0.35). (4) The heritabilities of cows with MAS, MET, and KET were found to be low, with values of 0.09, 0.01, and 0.02, respectively. The genetic correlation between these traits ranged from 0.14 to 0.44. This study offers valuable insights on the prevention and control of the three diseases, as well as feeding management and genetic breeding.

Evaluation of forage quality in various soybean varieties and high-yield cultivation techniques

ContextThe rising demand for animal protein has intensified forage shortages and restricted pasture availability, underscoring the critical need for effective dual-purpose animal feeds. ObjectiveThis study aims to evaluate the potential of vegetative soybeans as animal feed by assessing their nutritional value and productivity. Specifically, it explores the feasibility of using late-maturing soybean varieties, adapted to low latitudes, as forage in high-latitude regions and examines the impact of different planting dates on their growth and effectiveness during limited growing seasons. MethodsTo achieve these objectives, a series of field and controlled experiments were conducted. The first experiment assessed 418 mid-maturing soybean varieties from the Huang-Huai-Hai Basin in China for their nutritional value as forage during 2020 and 2021. The second experiment tested late-maturing and photoperiod-sensitive soybean varieties, including "ZGDD" and the methionine-enhanced material "Ox-CGS," under tropical conditions with extended daylight. The third experiment evaluated forage soybeans sown on June 19th, July 14th, and August 8th, 2022, to determine their yield and protein content. ResultsResults showed that the first experiment yielded an average protein content of 24.7 %, ether extract content of 1.9 %, neutral detergent fiber content of 41.9 %, and acid detergent fiber content of 27.3 %, with a relative feeding value of 153, surpassing local standards for high-quality leguminous forage. The second experiment demonstrated that extended daylight increased crude protein yield by 3.7 times compared to Chinese soybean seed yields, and "Ox-CGS" showed a 24.2 % increase in methionine content. The third experiment revealed that forage soybeans achieved a crude protein yield of 0.95 t/ha within 60 days, exceeding the 2022 average of 0.68 t/ha for seed soybeans in China. ConclusionsThe findings indicate that photoperiod-sensitive soybeans can be effectively used as forage over extended periods, utilizing artificial lighting or cultivation in higher latitudes, and that short growth cycles enhance crude protein accumulation. Implications or significanceThis research highlights the potential of soybeans to alleviate forage shortages, improve land use efficiency, and contribute to food security and agricultural development, especially on marginal lands and in regions with limited growing seasons.

Dry fractionation of chickpea flour: Impact of de-oiling and flow aids

Air classified chickpea protein concentrates often lack the separation efficiency of other starch-rich pulses. We subjected chickpea flour to de-oiling or to blending with different types and concentrations of silica flow aids to reduce its cohesion and to increase its dispersibility in air. Air classification enriched protein to a content of 45%. Initial protein yields below 25% increased to 70% upon extensive de-oiling, and this de-oiled ingredient containing 51% protein was enriched to 56% by electrostatic separation. Alternatively, nanosized flow aids doubled the initial protein yield. SEM-EDS image analysis revealed that higher silica concentrations increased the accumulation of nanoparticles onto flour particles, which lowered the flour's basic flow energy and moderately increased its dispersibility. This reduced fouling during air classification, but the protein yield levelled off with increasing silica concentrations because the microstructure was not disentangled further. Silica addition during milling rendered post-milling blending unnecessary, without compromising the protein yield.

Effects of partial or full replacement of soybean meal with urea or coated urea on intake, performance, and plasma urea concentrations in lactating dairy cows.

We expected mitigation of the hypophagic effects of urea (U) with a coated urea (CU) product that aimed to partially shift urea supply to the post-ruminal gastrointestinal tract. Ruminal release and post-ruminal digestibility of CU was evaluated in vitro, followed by a randomised complete block experiment (54 Holstein-Friesian cows; 177 ± 72 days in milk). Soybean meal (SBM) was partially (PR) or fully (FR) replaced on an isonitrogenous basis by beet pulp and U or CU. Urea sources were included at 12 (U-PR, CU-PR) and 19 (U-FR, CU-FR) g/kg dietary dry matter (DM). Hypophagic effects were similar for U-PR and CU-PR (-11% vs. -7%), and for U-FR and CU-FR (-13% vs. -12%) compared with SBM (average 25.8 kg DM intake/d). Compared with SBM, U-PR and CU-PR reduced yields of milk (-8%) and protein (-12%), U-PR reduced yield of fat (-9%) and fat- and protein-corrected-milk (FPCM; -9%), and CU-PR tended to reduce FPCM yield (-5%). Compared with SBM, U-FR and CU-FR respectively reduced yields of milk (-21%, -22%), protein (-25%, -26%), fat (both -14%), lactose (-20%, -21%), and FPCM (-17%, -19%), and lowered N (-15%, -12%) and feed (-8%, trend, -9%) efficiency. Human-edible protein efficiency approximately doubled with U-PR and CU-PR and approximately tripled with U-FR and CU-FR compared with SBM. Milk composition and plasma urea concentration were similar between U and CU, except for a trend for a greater plasma urea concentration with U-PR compared with CU-PR. Dry matter intake patterns differed for CU-PR compared with U-PR and for CU-FR compared with U-FR, suggesting effects of urea release rate or location on feeding behaviour. Overall, replacing SBM with U or CU reduced DM intake and milk production and affected nutrient efficiencies. Coated urea influenced DM intake pattern but did not affect total DM intake or milk production compared with U.

Mammary gland responses to altering the supply of de novo fatty acid substrates and preformed fatty acids on the yields of milk components and milk fatty acids

The objective of our study was to evaluate the effect of altering the dietary supply of acetate, palmitic acid (PA), and cottonseed on the yields of milk components and milk fatty acids (FA) in lactating dairy cows. Thirty-two multiparous Holstein cows (133 ± 57 d in milk, 50.5 ± 7.2 kg/d milk) were used in a 4 × 4 Latin square split plot design with a 2 × 2 factorial arrangement of subplot treatments. Cows were blocked by energy-corrected milk (ECM) yield and allocated to a main plot receiving a basal diet (n = 16) with no supplemental PA (Low PA) or a basal diet (n = 16) with 1.5% inclusion of a FA supplement containing ~85% PA (High PA). In each main plot, the following subplots of treatment diets were fed in a Latin square arrangement consisting of 14-d periods: 1) a control diet (CON), 2) the control diet supplemented with 3% sodium acetate (AC), 3) the control diet supplemented with 12% whole cottonseed (CS), and 4) the control diet supplemented with 3% sodium acetate and 12% whole cottonseed (CS+AC). The PA supplement and sodium acetate replaced soyhulls, and whole cottonseed replaced cottonseed hulls and meal. All diets were balanced for 30% neutral detergent fiber (NDF), 23% forage NDF, 28% starch, and 17% crude protein (CP). Sources of FA were classified as de novo (<16 carbons), mixed (16-carbon), and preformed (>16 carbons). The statistical model included the random effect of cow nested within basal diet and fixed effect of period, basal diet, acetate, cottonseed, and their interactions. Three-way interactions among basal diet, acetate, and cottonseed were observed for the yields of milk fat, 3.5% fat-corrected milk (FCM), and the molar yields of de novo FA, mixed FA, and preformed FA. In the Low PA diets, AC and CS+AC increased the yields of milk fat and FCM compared with CON and CS, whereas, in the High PA diets, CS+AC increased the yields of milk fat and FCM compared with the other treatments and AC increased milk fat yield compared with CON and CS. Compared with Low PA, High PA increased milk fat content, mixed FA yield, and tended to increase C4:0 yield. Diets containing acetate increased DMI and the yields of milk fat, ECM, FCM, de novo FA, mixed FA, and preformed FA compared with diets without acetate. Diets containing cottonseed increased the yields of milk and preformed FA, tended to increase the yields of FCM and protein, and decreased DMI and the yields of de novo FA and mixed FA compared with diets without cottonseed. In summary, in high PA diets, the inclusion of acetate plus cottonseed increased milk fat yield compared with the other treatments. The CON diet in High PA increased milk fat yield to the same extent as AC and CS+AC in Low PA suggesting PA is important for initiating milk TG synthesis. Balancing the supply of de novo FA substrates and preformed FA is important for increasing the synthesis of milk fat triglycerides and milk fat production.

Maltodextrin concentration on the encapsulation efficiency of tempeh protein concentrate from Jack Bean (Canavalia ensiformis): physical, chemical, and structural properties

ABSTRACT Jack Bean tempeh, a highly nutritious protein source, is underutilized in the food industry. As a protein concentrate, the protein hydrolyzate has a limited shelf life, necessitating encapsulation for preservation. Encapsulation, mainly through freeze-drying, is a promising solution. This study, which explores the impact of different maltodextrin concentrations on the physical and chemical properties of encapsulated Jack Bean protein concentrate, has the potential to advance food technology and nutrition significantly. The methodology involved descriptive analysis and the addition of maltodextrin at three distinct concentrations: 10%, 20%, and 30% (w/w), relative to the weight of the ingredients. Meanwhile, the parameters investigated included protein content, yield, total solids, water and oil absorption, encapsulation effectiveness, and microstructure, which were studied using Scanning Electron Microscopy (SEM). The results revealed that the concentration of maltodextrin significantly influenced the encapsulation process. The protein content, water absorption, oil absorption, porosity, and encapsulation efficiency reached their peak at a maltodextrin concentration of 10%, with values of 27.26 ± 0.18%, 2.20 ± 0.05 g/g, 2.15 ± 0.02 ml/g, 0.52 ± 0.5%, and 73.19 ± 0.3%, respectively. Conversely, a 30% concentration of maltodextrin led to an increase in the yield to 61.33 ± 0.35% and the total solids to 92.52 ± 0.13%. The freeze-drying process resulted in non-uniform shapes in the microstructure in all treatments.

Parent of origin genetic effects on milk production traits in a population of Iranian Holstein cows.

The aim was to estimate the relative contribution of imprinting effects from both paternal and maternal sides to phenotypic variation in milk production traits including 305 days milk yield (MY), average daily milk production (ADM), fat percentage (F%), protein percentage (P%), 305 days fat yield (FY), 305 days protein yield (PY), ratio of fat percentage to protein percentage (F:P) and somatic cell score (SCS) in Iranian Holstein cows. To do this, each trait was analysed with a series of four animal models, which were identical for fixed and additive genetic effects but differed for combinations of paternal and maternal imprinting effects. The log-likelihood ratio test (LRT) and Akaike's information criteria (AIC) were used to select the best model for each trait. Correlations between traits due to additive and imprinting effects were estimated by bivariate analyses. For all traits studied, fitting the imprinting effect led to a better data fit. Also, it resulted in a noticeable decrease in additive genetic variance from 8% (SCS) to 28% (F:P). A significant maternal imprinting effect was detected on all traits studied. Estimates of maternal imprinting heritability ( ) were 0.07 ± 0.02, 0.04 ± 0.01, 0.06 ± 0.01, 0.05 ± 0.01, 0.5 ± 0.01, 0.09 ± 0.02, 0.07 ± 0.02 and 0.06 ± 0.01 for MY, ADM, F%, P%, FY, PY, F:P and SCS, respectively. For F:P, in addition to the maternal imprinting effect, a significant paternal imprinting component was also detected with a 7% contribution to phenotypic variance of F:P. Estimates of direct heritability ( ) were 0.29 ± 0.02, 0.17 ± 0.01, 0.22 ± 0.02, 0.11 ± 0.01, 0.18 ± 0.02, 0.22 ± 0.02, 0.15 ± 0.04 and 0.06 ± 0.01 for MY, ADM, F%, P%, FY, PY, F:P and SCS, respectively. Maternal imprinting correlations (rmi) were in a wide range between -0.75 ± 0.15 (P%-SCS) and 0.95 ± 0.11 (MY-ADM). Additive genetic correlations (ra) ranged between -0.54 ± 0.05 (MY-P%) and 0.99 ± 0.01 (MY-ADM) and phenotypic correlations (rp) ranged from -0.30 ± 0.01 (MY-F%) to 0.93 ± 0.01 (MY-ADM). The Spearman's correlation between additive breeding values including and excluding imprinting effects deviated from unity especially for top-ranked animals implying re-ranking of top animals following the inclusion of imprinting effects in the model. Since including imprinting effects in the model resulted in better data fit and re-ranking of top animals, including these effects in the genetic evaluation models for milk production traits was recommended.

Influence of Nitrogen Fertilization on Quality Parameters and Heat Use Efficiency of Basmati Rice

ABSTRACT Physical, milling, cooking, and chemical qualities of basmati cultivars had great concern for researchers, producers, traders, and consumers as instability index of production is higher. Quality may be enhanced by good N optimization to responsive basmati varieties. Therefore, a field experiment was conducted for two consecutive years under split-plot design. Results revealed that application of 120 kg N ha−1 improved heat use efficiency (4.85 & 4.91 kg ha−1 degree-day−1), milling quality traits, physical quality, and chemical quality while aroma score (up to 2.50) improved only up to 60 kg N ha−1. Highest protein (7.79 & 7.77%) and least amylose (21.88 & 22.24%) content were recorded with highest N rates while highest protein yield (414.18 & 402.66 Kg ha−1) reported at 120 Kg N ha−1. Basmati varieties, HUBR 10–9 and HUBR 2–1, were superior in most of quality traits along heat use efficiency (4.62–4.70 kg ha−1 degree-day−1). However, short-grained aromatic variety HUR 917 recorded higher head rice recovery (64.34 & 63.21%), protein (7.61 & 7.63%), and amylose content over others. Most important trait, i.e. aroma (1.83–2.38) score, was higher with basmati varieties. Overall, basmati (HUBR 10–9 and HUBR 2–1) grown with 120 kg N ha−1 proved superiority. This study achieved better heat use efficiency along with improved quality of basmati rice which found to be positively correlated with most quality traits of basmati rice. The low amylose in basmati is preferable, and amylose found to be negatively correlated with heat use efficiency.

Response surface methodology guided approach for optimization of protein isolate from Faba bean. Part 1/2

Ultrasound-assisted extraction (UAE) was evaluated as a green procedure to produce faba beans protein isolates from faba beans. Magnetic stirring was performed as conventional extraction. A three-level five-factor Box-Behnken Design (BBD) was applied to obtain the optimal UAE conditions to concurrently maximize extraction yield and protein content. The response surface methodology (RSM) showed a quadratic curvature for extraction yield and protein. The optimal extraction conditions were determined as: Power of 123 W, solute/solvent ratio of 0.06 (1:15 g/mL), sonication time of 41 min, and total volume of 623 mL with a desirability value of 0.82. Under these conditions, the extraction yield of 19. 75 ± 0.87 % (Protein yield of 67.84 %) and protein content of 92.87 ± 0.53 % were obtained for optimum ultrasound extraction. Control samples using magnetic stirring under similar conditions without ultrasound treatment showed an extraction yield of 16.41 ± 0.02 % (Protein yield of 54.65 %) and a protein content of 89. 88 ± 0.40 %. This shows that BBD can effectively be used to optimize the extraction of proteins from faba beans using optimal extraction conditions, resulting in a higher extraction yield and protein purity.

Understanding the protein extraction potential of tomato leaves (Solanum lycopersicum) through mass balance modelling

Tomato leaves, the main by-product from tomato production, are a potential protein source. In this study, proteins were extracted from tomato leaves collected from different positions of the plant (top, middle and bottom) using three different purification methods: acid precipitation, ethanol precipitation and dialysis. The protein extraction process was evaluated in terms of protein yield and purity. In addition, mass balances were established to estimate the potential additional protein recovery as well as to identify the limiting factors of the extraction. Significant protein loss (0.62 g/g total proteins) occurred during the initial processing steps, which was mainly attributed to the water absorbed by the fibrous pulp and the presence of large quantities of insoluble proteins. Part of the loss may be recovered from the pulp and thus considered attainable. However, most of these proteins were inevitably lost, due to their insolubility. In general, low protein yields were found for all leaves when aiming for a protein extract with high purity, with some variations in different leaves. Top (young) leaves resulted in the highest protein yield, suggesting the presence of more intact proteins. Acid precipitation resulted in the highest protein purity, suggesting a high selectivity for proteins.