Proficiency Testing Research Articles

The Effect of Using Programmed Instruction on Students' Learning of Javelin Effectiveness on Third-Year Students at Department of Physical Education and Sports Sciences

Abstract:Teaching methods that actively engage the student and entrust him with learning tasks are among the important and successful methods of delivering the educational material to the student, and on this basis the researcher set out to test programmed education that allows the student to be effective and able to make decisions and advance learning according to his capabilities, and through the researcher's modest experience in Teaching the field and field subject I noticed the weakness of the skill performance of the third stage students in the javelin event because of the difficulty of learning that is characterized by this event, and to solve this problem the researcher used linear programmed education through designing an educational CD that displays the educational material and exercises that the student follows to learn performance The skills to see them and progress in their studies and apply exercises according to the speed of his learning and his possibilities to reach learning, under the supervision of the subject teacher. Where the researcher believes that the use of programmed learning may increase students' ability to achieve better learning through reading, follow-up, and performing exercises in the educational CD. The aim of the research is to identify the effect of using the programmed teaching method on learning the skill performance of the effectiveness of javelin throwing among students of the third stage / Department of Physical Education / College of Basic Education / University of Diyala. As for the research hypotheses, they were represented by the existence of statistically significant significant differences between each of the following: 1. The proficiency test before and after and for the control and experimental groups, in favor of the post test. 2. The post-skill test for the control and experimental groups, for the benefit of the experimental group. The researcher used the experimental method by designing the control and experimental groups for its suitability and the nature of the problem, as she selected the research community by the intentional method, which is the third stage in the Department of Physical Education, College of Basic Education. The research sample was represented by (15) students representing the experimental group and (15) students representing the control group. The control group studied using the traditional method, while the experimental group studied using programmed education. As for the most important findings of the research, they were thus represented 1- Programmed education has an effect on learning skill performance better than the used method. 2- The interaction of students in the experimental group in viewing and following up on the prepared tablet according to the programmed teaching. As for the most important recommendations, they were: - 1- Preparing educational tablets according to the programmed education for each of the square and field activities. 2- Using programmed education in games and other skills other than the field and the field

Evaluating methodological enhancements to the Yes/No Angoff standard-setting method in language proficiency assessment

This study evaluated the efficacy of two proposed methods in an operational standard-setting study conducted for a high-stakes language proficiency test of the U.S. government. The goal was to seek low-cost modifications to the existing Yes/No Angoff method to increase the validity and reliability of the recommended cut scores using a convergent mixed-methods study design. The study used the Yes/No ratings as the baseline method in two rounds of ratings, while differentiating the two methods by incorporating item maps and an Ordered Item Booklet, each of which is an integral tool of the Mapmark and the Bookmark methods. The results showed that the internal validity evidence is similar across both methods, especially after Round 2 ratings. When procedural validity evidence was considered, however, a preference emerged for the method where panelists conducted the initial ratings unbeknownst to the empirical item difficulty information, and then such information was provided on an item map as part of the Round 1 feedback. The findings highlight the importance of evaluating both internal and procedural validity evidence when considering standard-setting methods.

The influence of English language proficiency test scores on the academic success of ESL undergraduate students

ABSTRACT This study considers language proficiency test scores achieved by ESL students who subsequently entered a New Zealand university at the undergraduate level. Scores from IELTS and the university’s in-house English Proficiency Test are analysed to determine the predictive ability of overall scores on these two tests and scores for each of the four language skills on academic success. The results indicated that language proficiency had a significant effect on undergraduate academic achievement. Furthermore, analyses of the predictive ability of scores for listening, speaking, and reading were remarkably similar between the tests, with non-significant results of similar magnitude for each skill. On the other hand, IELTS writing scores demonstrated a non-significant negative effect on academic achievement, while EPT writing scores were strongly significantly predictive of academic achievement. The results suggest that universities may benefit from measuring ESL students’ academic writing proficiency in more authentic university-level writing tasks.

External proficiency testing exercises: challenges and opportunities.

External proficiency testing exercises: challenges and opportunities.

Laboratorian Interpretation of Drug Testing Results in Pain Management: Lessons From College of American Pathologists Proficiency Testing.

Accurate interpretation of drug test results is key to appropriate patient care in numerous settings including pain management. Despite recommendations that providers should consult laboratory professionals for guidance when necessary, literature demonstrating laboratorian expertise in drug test interpretation is lacking. To evaluate participating laboratories' performance on the case-based, interpretive ("dry") challenge included with each Drug Monitoring for Pain Management proficiency testing program from 2012-2023. All challenges (n = 23) required participants to identify if drug test results were consistent or inconsistent with prescribed medications in the case history. Relevant medications, presumptive and confirmatory drug test results, and participant responses were extracted from program summary reports and examined for performance and common themes. Overall, 91.8% (6821 of 7431) of participant responses correctly identified whether drug testing was consistent with medications. There were 8 challenges with participant scores below 91.8% (range, 59.8% [49 of 82 responses] to 88.9% [193 of 217 responses]). Common knowledge gaps identified in these challenges included false-positive presumptive (screening) results, minor metabolism of opiates, and recognizing that presence of a nonprescribed drug is inconsistent with prescribed medications. Although some participants repeatedly responded incorrectly, there were no associations between laboratory type, personnel responding, or analytical performance with incorrect responses to interpretative challenges. Program participants performed well overall, but several concerning educational gaps were identified. Laboratorians have a role in providing interpretative guidance for drug testing and should emphasize ongoing education to ensure competence in the setting of constantly changing prescribed and nonprescribed drug use.

Determination of urea in pet feed: assessing the suitability of different analytical techniques using proficiency test data

The determination of urea in pet feed at contaminant levels using the spectrophotometric method described in Commission Regulation (EC) No 152/2009 has been reported by several EU laboratories to lack the required selectivity. Whilst urea is not authorised as an additive in pet feed, the control of urea in pet feed is of economic importance, because the addition of urea may unlawfully increase the apparent protein content. To investigate the capabilities of different analytical techniques, a proficiency test was organised where the participants (EU official control laboratories, laboratories from the academia and private laboratories) were free to use their method of choice for analysing three dog feed test materials, two samples of which were spiked with urea. Twenty-one laboratories submitted results using the following techniques: spectrophotometry (Implementing Regulation (EC) No 152/2009), LC-MS/MS, HPLC-UV, enzymatic-colorimetry, gravimetry and an ‘in-house photometric’ method. Only two laboratories that used LC-MS/MS were able to quantify urea accurately in the test material containing a mass fraction of 18.9 mg kg−1 whereas satisfactory results at the level of 258.9 mg kg−1 were obtained by one participant that used an ‘in-house photometric method’ and one that used the enzymatic method, in addition to the five participants using LC-MS/MS. The technique that provided the highest success rate across the three test materials was LC-MS/MS, whereas spectrophotometry, the enzymatic-based and HPLC-UV methods led to overestimated results in addition to a dispersion of results not suitable for compliance analysis. To address the determination of urea in pet feed at low levels, a better performing method than the one described in the legislation is required.

Improvement of laboratory testing services by implementation of quality assurance scheme

Introduction: The National AIDS Control Organization (NACO), Government of India and the Uttar Pradesh State AIDS Control Society (UPSACS) established the State Referral Laboratory (SRL) in the Department of Microbiology, Motilal Nehru Medical College, Prayagraj for HIV testing and monitoring of linked HIV Counselling and Testing Services (HCTS) in Uttar Pradesh.Methods: This is a retrospective study conducted from 2008 to 2021. In this evaluation period 10144 serum samples (9580 HIV negative, 555 HIV positive and 9 discordant) were received for retesting at SRL. The Quality Control serum samples received from testing sites consisted of 5% negative and 20% positive from all tested samples collected in the first seven days in the External Quality Assurance Scheme (EQAS) quarter month i.e. January, April, July and October. All samples were tested using rapid diagnostic kits procured by NACO/UPSACS with NACO testing strategy. All linked centres with SRL received 4 coded serum samples for proficiency testing (PT) which were tested similarly.Results: Of 10144 serum samples, 99.92% reported correct results and 0.08% reported discrepancy due to various issues. Similarly in the proficiency testing programme, of 4072 samples tested between 2010 to 2021 by testing sites, 4061 (99.07%) reported concordant results and the remaining14 (0.34%) samples reported indeterminate results. Of the 4072 samples, 14 shows discordant results by three different rapid tests based on different principles. Hands on training were provided for technicians who reported indeterminate results in PT or retesting.Conclusion: The tremendous increase in quality testing at peripheral centrally linked centres increase the reliability of HIV diagnosis at ground level. The study also concluded that continuous monitoring and mentoring of technicians of the diagnostic laboratories under EQAS improves their quality for HIV diagnosis.

IgE Antibody Assay-Based Allergen Database(CLSI-IL/A-37)

There are 8 established allergen databases (IUIS/WHO ANDB, Allergome, AllFam, AllergenOnline, SDAP, COMPARE, IEDB and AllerBase).None of these provide a comprehensive listing of the internationally-accepted nomenclature codes and Linnean system descriptors of the~1000 extract and molecular allergens used world-wide in diagnostic single-and multi-plex IgE antibody assays. The new Diagnostic Allergen DataBase (DADB) has been prepared by an international scientific committee of the Clinical and Laboratory Standards Institute to specifically provide these data together in a readily-searchable Excel database. Using peanut as an example, the DADB lists the allergens’ unique assay working codes (F13), general category (food), principal IgE antibody assay method(s) where the allergen specificity is available, common name (peanut), taxonomical name (Arachis hypogaea), and the NCBI, NPU, LOINC, Allergome and IUIS/WHO descriptor codes for allergens and their assays. The DADB benefits manufacturers of allergen-specific IgE assays by providing unambiguous specificity descriptors. With the DADB, allergen extract manufacturers will have clearly defined specificity targets for allergenic product development. Government regulators will have clarity on the specificity of new allergen reagents that are being submitted for clearance. IgE antibody proficiency testing programs (e.g. CAP) will be able to clearly define the IgE antibody specificities being measured in their challenge sera. The clinician will precisely know the allergen specificity of IgE antibody that is measured in their reference laboratory. Finally, the allergic patient will be assured that their allergic sensitization is being assessed in an assay with a well-characterized allergen reagent.

Lesson Learned from the Initial Phase of the Development Process of CEFR-Based Uttaradit Rajabhat University Test of English Proficiency

Background and Aims: The development of the CEFR-Based Test of English Proficiency enriches the broader field of language assessment. By sharing valuable insights into the complexities of aligning assessments with international frameworks. Thus, this academic article aims to present the lesson learned during the initial phase of the development process of the CEFR-Based Uttaradit Rajabhat University Test of English Proficiency (URU-TEP). Linking the URU-TEP to the Common European Framework of Reference for Languages (CEFR), widely used as a reference in English as a Foreign Language (EFL) education, is crucial if test results are to be interpreted under the CEFR levels. Methodology: This study used the method of studying documents and related research, using thematic analysis and presenting descriptive information. Results: the finding is divided into four sections: the current state of the English proficiency test at URU, the process of developing the five-skill URU-TEP based on the CEFR, the process of obtaining test validity and reliability, and opportunities and challenges. Conclusion: The essence of the discovery is to enhance understanding in various areas. of the English proficiency testing process at Uttaradit Rajabhat University It also emphasizes consideration of the validity and reliability of the test to support quality and improve the examination process in the future.

Technical evaluation and standardization of the human thyroid microtissue assay.

The success and sustainability of U.S. EPA efforts to reduce, refine, and replace in vivo animal testing depends on the ability to translate toxicokinetic and toxicodynamic data from in vitro and in silico new approach methods (NAMs) to human-relevant exposures and health outcomes. Organotypic culture models employing primary human cells enable consideration of human health effects and inter-individual variability but present significant challenges for test method standardization, transferability, and validation. Increasing confidence in the information provided by these in vitro NAMs requires setting appropriate performance standards and benchmarks, defined by the context of use, to consider human biology and mechanistic relevance without animal data. The human thyroid microtissue (hTMT) assay utilizes primary human thyrocytes to reproduce structural and functional features of the thyroid gland that enable testing for potential thyroid-disrupting chemicals. As a variable-donor assay platform, conventional principles for assay performance standardization need to be balanced with the ability to predict a range of human responses. The objectives of this study were to (1) define the technical parameters for optimal donor procurement, primary thyrocyte qualification, and performance in the hTMT assay, and (2) set benchmark ranges for reference chemical responses. Thyrocytes derived from a cohort of 32 demographically diverse euthyroid donors were characterized across a battery of endpoints to evaluate morphological and functional variability. Reference chemical responses were profiled to evaluate the range and chemical-specific variability of donor-dependent effects within the cohort. The data-informed minimum acceptance criteria for donor qualification and set benchmark parameters for method transfer proficiency testing and validation of assay performance.

How do gene mutation diversity and disease severity scoring affect physical capacity and quality of life in children/adolescents with Familial Mediterranean Fever?

ObjectivesThe aim of this study is to examine how gene mutation diversity and disease severity affect physical capacity and quality of life in children/adolescents with Familial Mediterranean Fever (FMF). MethodsEighty children/adolescents (42 female, 38 male) diagnosed with FMF according to Tell-Hashomer diagnostic criteria were included in this study. Disease severity score (PRAS), running speed and agility and strength subtests of Bruininks-Oseretsky Test of Motor Proficiency Second Edition Short Form (BOT-2 SF), Physical Activity Questionnaire, Pediatric Quality of Life Inventory 3.0 Arthritis Module (PedsQL) was used for evaluation. Participants were divided into 2 groups as M694V and other mutations according to MEFV gene mutation and were divided into 3 groups as mild, moderate and severe according to PRAS. ResultsWhen the data were compared between groups; in terms of gene mutation, a significant difference was observed in treatment subtest of PedsQL-parent form in favor of the M694V gene mutation group (p<0.05). In terms of PRAS, significant difference was seen in the pain, treatment subtests and total score of the PedsQL-child form, and in the pain, treatment, worry subtests and total score of the PedsQL-parent form in favor of the mild group (p<0.05). ConclusionsMEFV gene mutations in children and adolescents with FMF did not differ on physical capacity and quality of life. PRAS was not effective on physical parameters, but quality of life decreased as the severity score increased. Encouraging children/adolescents with FMF to participate in physical activity and to support them psychosocially can be important to improve their quality of life.

Evaluation of the effects of lyophilization and post-reconstitution stability of plasma samples containing PT, APTT, and Fibrinogen parameters

Objective: To evaluate the effects of lyophilization and post-reconstitution stability of plasma samples containing PT (Prothrombin Time), aPTT (activated Partial Thromboplastin Time), and fibrinogen parameters. Research method: Experimental study was conducted on lyophilized plasma samples containing PT, aPTT, and fibrinogen parameters. The homogeneity of sample set before and after lyophilization was evaluated, then the stability of the sample set was assessed in variable time and temperature reservation conditions by Paired–samples t–test. Results: Plasma samples containing 3 parameters PT, aPTT and fibrinogen were not significantly affected by the lyophilization process, shown through repeated measurements with p-value of 0.912, 0.699 and 0.334 respectively. Lyophilized plasma samples after reconstitution are stored at -20°C to achieve stability for 8 weeks, at 2 - 8°C stable for 24 hours, at laboratory temperature stable for 8 hours with all PT, aPTT, and fibrinogen parameters. Conclusion: The freeze-drying process does not change the values of PT, aPTT, fibrinogen parameters in plasma samples, so it can be applied to produce coagulation proficiency testing samples by lyophilization. The reconstituted sample is stable in different time and reservation conditions, met the requierment of reservation condition of reconstituted sample. Key words: Lyophilization, reconstitution, PT, aPTT, fibrinogen.

Advancing precision in histocompatibility and immunogenetics: a comprehensive review of the UCLA exchange program.

Precise typing of human leukocyte antigens (HLA) is crucial for clinical hematopoietic stem cell and solid organ transplantations, transfusion medicine, HLA-related disease association, and drug hypersensitivity analysis. The UCLA Cell Exchange program has played a vital role in providing educational and proficiency testing surveys to HLA laboratories worldwide for the past 5 decades. This article highlights the significant contribution of the UCLA Cell and DNA Exchange Programs in advancing HLA antibody testing, genotyping, crossmatches, and, more recently, virtual crossmatches. Additionally, we discuss future directions of the UCLA Cell Exchange program to support histocompatibility testing to adapt to the fast-evolving field of immunotherapy, tolerance and xenotransplantation.

Distribution of the C9orf72 hexanucleotide repeat expansion in healthy subjects: a multicenter study promoted by the Italian IRCCS network of neuroscience and neurorehabilitation.

High repeat expansion (HRE) alleles in C9orf72 have been linked to both amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD); ranges for intermediate allelic expansions have not been defined yet, and clinical interpretation of molecular data lacks a defined genotype-phenotype association. In this study, we provide results from a large multicenter epidemiological study reporting the distribution of C9orf72 repeats in healthy elderly from the Italian population. A total of 967 samples were collected from neurologically evaluated healthy individuals over 70 years of age in the 13 institutes participating in the RIN (IRCCS Network of Neuroscience and Neurorehabilitation) based in Italy. All samples were genotyped using the AmplideXPCR/CE C9orf72 Kit (Asuragen, Inc.), using standardized protocols that have been validated through blind proficiency testing. All samples carried hexanucleotide G4C2 expansion alleles in the normal range. All samples were characterized by alleles with less than 25 repeats. In particular, 93.7% of samples showed a number of repeats ≤10, 99.9% ≤20 repeats, and 100% ≤25 repeats. This study describes the distribution of hexanucleotide G4C2 expansion alleles in an Italian healthy population, providing a definition of alleles associated with the neurological healthy phenotype. Moreover, this study provides an effective model of federation between institutes, highlighting the importance of sharing genomic data and standardizing analysis techniques, promoting translational research. Data derived from the study may improve genetic counseling and future studies on ALS/FTD.

Determination of N,N-Dialkylethanolamines Related to Chemical Warfare Agents as Benzylated Species in an OPCW Proficiency Test Soil Sample by Electron Ionization Gas Chromatography-Mass Spectrometry

In the field of chemical warfare agents (CWAs), ethanolamines are important building blocks for the preparation of nerve agents belonging to the V-series such as VX and VR as well as the nitrogen-based mustards. In this work, the determination of three members in this class of Schedule 2B reportable chemicals, namely N,N-dimethylethanolamine (DMEA), N,N-diethylethanolamine (DEEA), and N,N-diisopropylethanolamine (DIEA) by electron ionization gas chromatography-mass spectrometry (EI-GC-MS) is presented. The three dialkylethanolamines were spiked in a high organic content soil matrix featured during the 45th Organization for the Prohibition of Chemical Weapons (OPCW) proficiency test (PT) at two separate concentrations each (1 and 10 µg/g separately), simulating values often encountered during these tests. The N,N-dialkylethanolamines were O-alkylated using benzyl bromide and sodium carbonate at 55 °C and were detected as their benzylated versions by electron ionization GC-MS. The benzylation of each dialkylethanolamine yielded derivatives with higher molecular weight and therefore different GC-MS profiles such as longer retention times and sharper peak chromatography relative to their unmodified counterparts. These characteristics enable the analytical chemist to detect and confirm these important CWA-related chemicals specifically in instances where they are present in low concentrations (i.e. <10 µg/g) among more abundant interferences.

Current status and perspectives of the quality system in histocompatibility laboratories in Poland.

Allogeneic transplantation is a multi-step process involving many clinicians and laboratory personnel working together to achieve a common goal-to maximize the recipients' chance of survival and to improve their quality of life. One of the key elements of the process is to ensure high quality, accuracy, and reliability of histocompatibility testing. This manuscript presents: the development and organizational principles of the national system of supervision and control of histocompatibility laboratories in Poland, problems faced by these laboratories, availabe proficiency testing schemes, as well as suggestions and prospects for the future raised by members of the Polish histocompatibility community.

Measuring Variability in Proctor Decision Making on High‐Stakes Assessments: Improving Test Security in the Digital Age

AbstractRemote proctoring, or monitoring test takers through internet‐based, video‐recording software, has become critical for maintaining test security on high‐stakes assessments. The main role of remote proctors is to make judgments about test takers' behaviors and decide whether these behaviors constitute rule violations. Variability in proctor decision making, or the degree to which humans/proctors make different decisions about the same test‐taking behaviors, can be problematic for both test takers and test users (e.g., universities). In this paper, we measure variability in proctor decision making over time on a high‐stakes English language proficiency test. Our results show that (1) proctors systematically differ in their decision making and (2) these differences are trait‐like (i.e., ranging from lenient to strict), but (3) systematic variability in decisions can be reduced. Based on these findings, we recommend that test security providers conduct regular measurements of proctors’ judgments and take actions to reduce variability in proctor decision making.

The phosphylated butyrylcholinesterase-derived tetrapeptide GlyGluSerAla proves exposure to organophosphorus agents with enantioselectivity.

We herein present for the first time the phosphylated (*) tetrapeptide (TP)-adduct GlyGluSer198*Ala generated from butyrylcholinesterase (BChE) with proteinaseK excellently suited for the verification of exposure to toxic organophosphorus nerve agents (OPNA). Verification requires bioanalytical methods mandatory for toxicological and legal reasons. OPNA react with BChE by phosphonylation of the active site serine residue (Ser198) forming one of the major target protein adducts for verification. After its enzymatic cleavage with pepsin, the nonapeptide (NP) PheGlyGluSer*AlaGlyAlaAlaSer is typically produced as biomarker. Usually OPNA occur as racemic mixtures of phosphonic acid derivatives with the stereocenter at the phosphorus atom, e.g. (±)-VX. Both enantiomers react with BChE, but the adducted NP does not allow their chromatographic distinction. In contrast, the herein introduced TP-adducts appeared as two peaks when using a stationary reversed phase (1.8µm) in micro-liquid chromatography-electrospray ionisation tandem-mass spectrometry (µLC-ESI MS/MS) analysis. These two peaks represent diastereomers of the (+)- and (-)-OPNA adducted to the peptide that comprises chiral L-amino acids exclusively. Concentration- and time-dependent effects of adduct formation with (±)-VX and its pure enantiomers (+)- and (-)-VX as well as with (±)-cyclosarin (GF) were investigated in detail characterising enantioselective adduct formation, stability, ageing and spontaneous reactivation. The method was also successfully applied to samples from a real case of pesticide poisoning as well as to samples of biomedical proficiency tests provided by the Organisation for the Prohibition of Chemical Weapons.

Language Assessment at a Thai University: A CEFR-Based Test of English Proficiency Development

The increasing popularity of the Common European Framework of Reference (CEFR) in non-native English-speaking countries has generated a demand for concrete examples in the creation of CEFR-based tests that assess the four main English skills. In response, this research endeavors to provide insight into the development and validation of a CEFR-based test aimed at evaluating undergraduate students’ English proficiency for placement tests and exit exams. The CEFR served as the framework for item development while Classical Test Theory informed the test evaluation process. A sample of 2,248 first-year students participated in Testing 1 and 3,655 first- and second-year students took part in Testing 2. The results of the analysis of the multiple-choice listening and reading tests indicated favorable levels of item difficulty and discrimination indices, as well as high reliability coefficients obtained from Cronbach’s alpha, Kuder-Richardson, and split-half reliability. The correlation and regression analyses revealed close relationships between the subtests and between each subtest and the total score, supporting the test’s criterion validity. The study also demonstrated significant predictive validity on TOEIC scores. The findings of this study offer implications for the development of university-level English proficiency tests that integrate CEFR levels and CTT analysis.

Quartet metabolite reference materials for inter-laboratory proficiency test and data integration of metabolomics profiling

BackgroundVarious laboratory-developed metabolomic methods lead to big challenges in inter-laboratory comparability and effective integration of diverse datasets.ResultsAs part of the Quartet Project, we establish a publicly available suite of four metabolite reference materials derived from B lymphoblastoid cell lines from a family of parents and monozygotic twin daughters. We generate comprehensive LC–MS-based metabolomic data from the Quartet reference materials using targeted and untargeted strategies in different laboratories. The Quartet multi-sample-based signal-to-noise ratio enables objective assessment of the reliability of intra-batch and cross-batch metabolomics profiling in detecting intrinsic biological differences among the four groups of samples. Significant variations in the reliability of the metabolomics profiling are identified across laboratories. Importantly, ratio-based metabolomics profiling, by scaling the absolute values of a study sample relative to those of a common reference sample, enables cross-laboratory quantitative data integration. Thus, we construct the ratio-based high-confidence reference datasets between two reference samples, providing “ground truth” for inter-laboratory accuracy assessment, which enables objective evaluation of quantitative metabolomics profiling using various instruments and protocols.ConclusionsOur study provides the community with rich resources and best practices for inter-laboratory proficiency tests and data integration, ensuring reliability of large-scale and longitudinal metabolomic studies.