Contamination Of Products Research Articles

A Cu/β-cyclodextrin/reduced graphene oxide nanocomposite for efficient and multi-aflatoxin detection in rice, ginger and bean samples.

Aflatoxins (AFs) are some of the most important mycotoxins or fungal toxins that cause contamination of food products and are considered a threat to human and animal health. An efficient Cu/β-cyclodextrin/reduced graphene oxide nanocomposite (Cu/β-CD/rGO) has been prepared and applied as a new solid-phase extraction adsorbent for the separation and preconcentration of four AFs (B1, B2, G1, and G2) using high-performance liquid chromatography with fluorescence detection (HPLC-FLD). The successful synthesis of the prepared nanocomposite was confirmed using Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and transmission electron microscopy (TEM). The impacts of pH, amount of adsorbent, sample volume, desorption solvent volume, and salt concentration on the recovery of AFs were precisely investigated and optimized by central composite design (CCD). Under the optimal conditions, the introduced method demonstrated good linearity in the range of 0.4-5.4, 0.08-1.08, 0.4-5.4, and 0.08-1.08 ng g-1 for AFs B1, B2, G1 and G2, respectively. The limits of detection and quantification for the four AFs were obtained in the range of 0.06-0.53 and 0.20-1.62 ng g-1, respectively. The accuracy of the method was evaluated using recovery measurements in spiked real samples such as rice, bean, and ginger samples, and satisfactory recoveries were obtained in the range of 83.5-109.0% with good precision (RSDs between 2.4 and 8.6%). The results of this research revealed that our developed method is sensitive, highly effective, and convenient to perform for the trace analysis of AFs in different real samples.

Antibacterial Effect of Lime Juice Against Streptococcus suis and Other Bacteria in Minced Pork.

Streptococcus suis is a significant zoonotic pathogen, capable of causing severe illnesses such as septicemia and meningitis in both swine and humans. Its transmission through pork consumption necessitates effective food safety measures. Lime juice, known for its antimicrobial properties, presents a potential alternative to reduce S. suis contamination in pork products. This study investigated the antibacterial efficacy of lime juice specifically against S. suis and its potential to reduce bacterial contamination in minced pork, aiming to determine optimal treatment parameters for mitigating S. suis transmission through pork consumption. Seven strains of S. suis representing serotypes known to cause zoonotic disease were cultured, and lime juice was prepared. The minimum inhibitory concentration and minimum bactericidal concentration tests consistently demonstrated the antibacterial effect of lime juice against S. suis. Survival curve analyses showed significant bacterial reduction within 15 min at 25% (v/v) lime juice concentration. In minced pork, lime juice caused a notable decrease in total bacteria and S. suis counts after 15 min. This study demonstrates the potential of lime juice as an antibacterial agent against a representative strain of S. suis in pork. However, the results also highlight that lime juice alone may not eliminate all viable bacteria. Therefore, incorporating lime juice treatment together with proper cooking practices remains crucial to ensure safe consumption of pork products.

Tuning antibacterial efficacy against Pseudomonas aeruginosa by using green AgNPs in chitosan thin films as a plastic alternative

Nanotechnology advancements have facilitated the development of eco-friendly strategies to combat bacterial infections caused by antibiotic-resistant pathogens. This study promotes a green method for the synthesis of silver nanoparticles (AgNPs) utilizing Eucalyptus globulus leaf extracts as an alternative to traditional colloidal AgNPs obtained through chemical synthesis, investigating their antibacterial efficacy against Pseudomonas aeruginosa and their impact on the expression of bacterial virulence factors (pyocyanin, pyoverdine, rhamnolipids). This work demonstrates that: i. while colloidal AgNPs showed ineffective up to 120 μM, green AgNPs had a bactericidal effect already at 20 μM, without impacting bacterial virulence factors at sub-inhibitory concentrations; ii. the polyphenolic shell surrounding green AgNPs could play a crucial role in the antibacterial mechanisms, with a pro-oxidant action confirmed by a greater sensitivity to hydrogen peroxide (H2O2); iii. AgNPs improved the antibacterial properties of chitosan when incorporated into thin films. Consequently, an environmentally friendly nanocomposite film with antibacterial and antibiofilm properties was produced, which holds promise for application in food packaging to mitigate the emergence of microbial contamination in food products.

Deciphering Cronobacter sakazakii pathogenicity: Exploring virulence factors and host interactions": A short review

Opportunistic human pathogen called Cronobacter spp. can cause severe illnesses in immunocompromised individuals and newborns. These species are mostly responsible for disorders like meningitis, necrotizing enterocolitis, and bacteremia because they can survive in very dry foods like PIF and have also been identified in various environmental niches, including plants. These species' contamination is ascribed to biofilms, which are biotic or abiotic surface communities. Because they build biofilms on a variety of surfaces, identifying and eliminating them is extremely difficult. For the purpose of creating efficient treatment interventions and preventative measures, it is essential to comprehend the processes of virulence and the relationship between this pathogen and its host. A variety of virulence factors, such as adhesion factors, invasion proteins, biofilm formation components, and secreted toxins, contribute to Cronobacter spp. pathogenicity. Genes involved in capsule formation—such as those involved in exopolysaccharide synthesis—are essential to its pathogenicity because they are critical in desiccation resistance and resistance to host immunological defenses. Furthermore, genes that encode flagella, fimbriae, and outer membrane proteins are linked to host cell adherence and invasion. This review also explores the emerging link between Cronobacter spp. and plants, highlighting the bacterium's ability to survive and persist in the environment because Plants may act as reservoirs, contributing to the contamination of raw agricultural products, which can subsequently enter the food supply chain. Developing focused preventative measures and treatment approaches requires knowledge of the various biological as well as genetic factors that contribute to Cronobacter spp. virulence and how it affects the host. This review clarifies the effects of Cronobacter spp. on the host and investigates the genes linked to virulence in Cronobacter.

Health risk assessment of lead and cadmium in milk-based products of Iran

The primary objective of this study is to assess the health risks associated with lead (Pb) and cadmium (Cd) contamination in dairy products consumed in Iran. To conduct the research, 132 samples of cow milk, yogurt, cream, and doogh (a traditional Iranian beverage) were collected from both traditional and industrial livestock farms in Zanjan, Iran. The samples were microwave-digested, and an atomic absorption spectrophotometer equipped with a graphite furnace was used to determine toxic metals contents. The findings of the study revealed that the average concentrations of Pb in milk and dairy product exceeded the permitted limits. The mean ± se levels of Pb and Cd in milk, yogurt, doogh, and cream were 92.64 ± 8.73, 52.19 ± 8.42, 78.80 ± 12.89, 106.07 ± 8.94 μg/kg and 2.34 ± 0.22, 1.21 ± 0.20, 2.59 ± 0.57, 5.61 ± 0.63 μg/kg, respectively. For the purpose of exposure assessment, the chronic daily intake (CDI) was utilized, while the Hazard Quotient (HQ) and Total Hazard Quotient (THQ) were employed for non-carcinogenic risk assessment. Additionally, the Incremental Lifetime Cancer Risk (ILCR) was utilized for carcinogenic risk assessment. The results revealed that the highest exposure to Pb and Cd in the P 95th of CDI were observed in milk, followed by yogurt, doogh, and cream. According to our finding, the HQ and THQ values for potentially toxic elements (PTEs) related to the consumption of dairy products in the P 95th of cumulative probability were below 1. The results indicate that the ILCR of Cd (P 95th) due to the consumption of dairy products falls within the acceptable risk range (10−4 to 10−6). While our findings indicate levels of Cd in dairy products, it is important to note that there are currently no established standards for permissible Cd concentrations in Iran. Therefore, further research and regulatory frameworks are needed to ensure public safety.

Impact of anticoccidial treatment with Solo papaya seed powder on haematological parameters in Sasso broilers Komlan Mawouli Wampah 1, 3, Kosi M

Coccidiosis is a highly contagious and damaging poultry disease. Despite its effectiveness against coccidiosis, chemotherapy has not been able to overcome certain difficulties, in particular the phenomenon of bioresistance acquired by the parasites, the rising price of anticoccidials, and the contamination of poultry products by toxic residues that are dangerous for consumers. As an alternative solution, Carica papaya is emerging as an interesting and less costly natural substance for eradicating coccidiosis. Our study aimed to evaluate the anticoccidial efficacy of the seed of Solo papaya and its effects on haematological parameters in Sasso broilers. Six-hundred-day-old chicks of Sasso breed, broiler type, were divided into four batches with five replicates per batch: untreated batch (T-), batch treated with amprolium 20% (T+), and two batches treated with papaya seed powder (5%), incorporated into the feed for one and two consecutive days per month, noted P1 and P2 respectively. Determination of number of eggs per gram (EPG) was based on Mc Master technique. Haematological parameters were determined from blood collected in tubes containing EDTA using Mindray BC-3000/China automated haematological analyser. Papaya seed powder Treatment showed anticoccidial efficacy, expressed by a significant drop in EPG in the treated batches. In addition, haematological parameters improved, notably an increase in haematocrit and a decrease in the rate of various leucocytes. Anticoccidial treatment with papaya seed powder (5%) resulted in an appreciable reduction in parasite load and an improvement in the haematological profile. The result application could serve as a sustainable alternative to synthetic chemical anticoccidial drugs.

Inactivation of Aspergillus Species and Degradation of Aflatoxins in Water Using Photocatalysis and Titanium Dioxide

Aflatoxins (AF) are highly toxic secondary metabolites produced by various species of Aspergillus, posing significant health risks to humans and animals. The four most prominent types are aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), and aflatoxin G2 (AFG2). These mycotoxins are prevalent in various environments, including water sources and food products. Among these mycotoxins, AFB1 is recognized as the most toxic, mutagenic, and carcinogenic to humans. Consequently, most efforts to mitigate the impact of AF have been focused on AFB1, with photocatalysis emerging as a promising solution. Recent research has demonstrated that using semiconductor photocatalysis, particularly titanium dioxide (TiO2), combined with UV–visible irradiation significantly enhances the efficiency of AF degradation. TiO2 is noted for its high activity under UV irradiation, non-toxicity, and excellent long-term stability, making it a favorable choice for photocatalytic applications. Furthermore, TiO2 combined with visible light has demonstrated the ability to reduce AF contamination in food products. This article summarizes the working conditions and degradation rates achieved, as well as the advantages, limitations, and areas of opportunity of these methodologies for the degradation of AF and preventing their production, thereby enhancing food and water safety.

Detection of micro- and nanoplastic particles in leafy green vegetables by SERS coupled with gold-silver core-shell nanoparticles.

Asensitive and accurate method has been developedfor detecting and quantifying polystyrene (PS) and polyethylene (PE) in food samples using surface-enhanced Raman spectroscopy (SERS) with a simple preparation process. Themethod is designed to effectively detect and quantify mixtures of these polymers in varying ratios within the food matrix. By employing gold-silver core-shell nanoparticles (Au@Ag NPs) as the enhancing substrate, the SERS method demonstrated superior sensitivity in detecting trace amounts of micro- and nanoplastic particles (MNPs). For 1-µm PS microparticles, the limit of detection (LOD) values range from 12 to 50 mg/L or mg/kg in water, spinach, and kale, while for 100-nm PS nanoparticles, the LOD values range from 18 to 47 mg/L or mg/kg. For 1-µm PE microparticles, the LOD values range from 173 to 416 mg/L or mg/kg in the same matrices, whereas for 65-nm PE nanoparticles, the values range from 446 to 744 mg/L or mg/kg. The mixtures of PS and PE in varying ratios were also tested, with both plastics detectable even at trace levels, emphasizing the method's precision in detecting plastic contaminants. These findings highlight the potential of SERS as a powerful tool for monitoring MNP contamination in food products by detecting both individual plastics and their mixtures, enabling precise quantification of contamination and contributing to improved food safety.

Characterization of two Campylobacter jejuni phages and evaluation of their antibacterial efficacy with EDTA.

Campylobacter jejuni is a leading cause of foodborne illness worldwide. The application of bacteriophages offers a promising approach to specifically target and reduce C. jejuni contamination in food products. In this study, two C. jejuni phages were characterized, and their ability to inhibit bacterial growth in combination with ethylenediaminetetraacetic acid (EDTA) was investigated. Both phages exhibited tolerance to a wide range of temperature (4-60°C) and pH (3-9). Phage vB_CjeM-PC10 and vB_CjeM-PC22 were found to have a latent period of 30 min and 20 min and a burst size of 7 and 35 PFU/cell, respectively. Phage vB_CjeM-PC10 has a linear double-stranded DNA (dsDNA) genome of 51,148 bp with 77 ORFs and 29% GC content. Phage vB_CjeM-PC22 has a circular dsDNA genome of 32,543 bp with 56 ORFs and 28% GC content. At 42°C, the combination of these phages (MOI = 10) and EDTA decreased the count of viable C. jejuni by 5.2 log10 and inhibited the regrowth of resistant cells for 48 h. At 4°C, phage vB_CjeM-PC10 alone (MOI = 1000) reduced the count of viable C. jejuni by 3 log10 in brain heart infusion (BHI) broth and 2 log10 on chicken skin after incubation for 48 h. Although these phages were effective against C. jejuni, they cannot be utilized directly for food safety applications because they are lysogenic. Nevertheless, these findings expand the genome library of C. jejuni phages and enrich data resources by highlighting potential strategies for controlling C. jejuni infections.

Chlorella vulgaris as a food substitute: Applications and benefits in the food industry.

Chlorella vulgaris, a freshwater microalga, is gaining attention for its potential as a nutritious food source and dietary supplement. This review aims to provide a comprehensive discussion on C. vulgaris, evaluating its viability as a food substitute in the industry by exploring the nutritional value and application of C. vulgaris in the food industry. Rich in protein, lipids, carbohydrates, vitamins, and minerals, Chlorella offers substantial nutritional benefits, positioning it as a valuable food substitute. Its applications in the food industry include incorporation into smoothies, snacks, and supplements, enhancing the nutritional profile of various food products. The health benefits of Chlorella encompass antioxidant activity, immune system support, and detoxification, contributing to overall well-being. Despite these advantages, the commercialization of Chlorella faces significant challenges. These include variability in antibacterial activity due to strain and growth conditions, high production costs, contamination risks, and sensory issues such as unpleasant taste and smell. Additionally, Chlorella can accumulate heavy metals from its environment, necessitating stringent quality control measures. Future prospects involve improving Chlorella strains through genetic manipulation to enhance nutrient content, developing cost-effective culture systems, and exploring advanced processing techniques like pulsed electric fields for better digestibility. Addressing sensory issues through flavor-masking strategies and employing environmental management practices will further support Chlorella's integration into the food industry. Although C. vulgaris shows great potential as a nutritious food ingredient, overcoming existing challenges and optimizing production methods would be crucial for its successful adoption and widespread use.

Quantification of toxic metals contamination and health risk assessment in processed and raw dairy products in Abbottabad city

Toxic metal contamination (such as Cd, Cr and Pb) in processed and raw dairy products is an important environmental and public health concern. The accumulation of these metals in dairy products causes health threats to consumers. Various anthropogenic and natural activities discharge metals into the soil, where they are consumed by plants and become a part of the food chain. In addition, there is a considerable risk of metal contamination in dairy products during various business processes. The present study was carried out to determine the quantities of toxic metals found in dairy product samples. These dairy products have been classified into two groups: raw dairy products, which were self-extracted from cows and buffaloes employing adequate hygiene, and processed dairy products, which were bought from the Abbottabad market. These samples were generated using a double acid digestion approach, and toxic metals were quantified using atomic absorption spectrophotometry (AAS). For health risk assessment, the Health Risk Index (HRI), Target Hazard Quotient (THQ), and the Target Cancer Risk (TCR) indices were used. The relative levels of toxic metals in processed dairy product samples were as follows: Cd levels were highest in cheese (0.106±0.006 mg/kg), followed by butter and desi ghee (0.089±0.066 and 0.074±0.043 mg/kg, respectively). Cr concentrations were maximum in flavored milk (0.471± 0.290 mg/kg) and desi ghee (0.371± 0.01 mg/kg). Pb levels found in cheese were higher (1.753±0.194 mg/kg), then desi ghee and butter (1.025±0.01 and 0.652±0.397 mg/kg, respectively). Toxic metals in raw dairy products were measured in cow samples in the order of Cr ≥ Pb ≥ Cd. In a similar manner for buffalo samples, toxic metals followed the same trend as in cow samples. Though the concentrations of targeted metals in both raw and processed dairy product samples exceeded the threshold levels, the estimated levels of THQ and TCR were within the permissible range. However, our findings suggest that these metals in dairy products should be assessed on a continuous basis, as anthropogenic activities are contributing substantially to metal contamination.

Microbial profile of biofilms formed inside recovered plastic bottles and contamination of food products therein conditioned: Case of Hibiscus sabdariffa L. beverage

Recovered plastic bottles are reused in Cameroon as food packaging for the conditioning of certain foods called “traditional” based on production processes and structures. However, the recycling process of these bottles does not ensure the complete removal of contaminants present in the bottles, particularly biofilms. In the present study, focus was made on the contamination of Hibiscus sabdariffa beverage by biofilms formed inside recovered plastic bottles. Recovered and ready-to-be-reused plastic bottles were sampled from traditional food producers. The presence of biofilms inside bottles was screened and the microbial profile of biofilms was assessed. H. sabdariffa beverages prepared under aseptic conditions were conditioned in recovered plastic bottles and stored for 35 days at 4°C. During storage, the microbiological and physicochemical quality of the beverages was followed each 7th day. Biofilms were found in all bottles analyzed and their microflora were made of microorganisms belonging to genera Salmonella, Shigella, Vibrio, Escherichia, Pseudomonas, Klebsiella, Staphylococcus and Candida, with the dominance of the genus Pseudomonas (37.10 %) and Pseudomonas aeruginosa (21.35 %) at the level of species. Amongst the 135 isolates screened, the greatest biofilm-forming abilities were recorded for E. coli P5 (0.62±0.02), Staphylococcus aureus P13 (0.67±0.05), Klebsiella pneumoniae P15 (0.66±0.03), and Vibrio cholerae P7 (0.67±0.01). The microbial loads of beverages stored at 4°C in recovered plastic bottles collected from the producers were all out of norm on day 7, thus suggesting a reduced shelf-life of up to 7 days. The presence of microorganisms in beverages conditioned in bottles cleaned in the lab while applying the good hygiene practices suggests that, although there was an improvement with significant reduction of biofilms and thus microbial contamination, further improvements of the cleaning process through response surface methodology are needed.

A dual-action strategy of propenyl guaethol: pilY-mediated biofilm inhibition and augmenting aminoglycoside antibiofilm activity against Pseudomonas aeruginosa through in vitro and in silico studies

Flavoring compounds are natural or synthetic substances that enhance the food flavor. Research studies have demonstrated that flavoring compounds may have biological activities. In food industry, P. aeruginosa dominates spoilage and contamination of food products. Human exposure to P. aeruginosa may lead to serious infections. P. aeruginosa forms complex biofilms with extracellular slime matrix, providing protection against antimicrobial agents. The present study investigates the role of a flavouring food additive, propenyl guaethol (PG) against Pseudomonas aeruginosa biofilms. Our results demonstrate a significant impact of PG on biofilm forming ability, bacterial attachment, and motility phenotypes. The polystyrene tube assay demonstrates notable inhibition of biofilm formation by P. aeruginosa at 50 and 25 µg/ml (p < 0.01). PG showed marked inhibition of biofilms in combination with gentamicin, kanamycin, and streptomycin. Additionally, PG inhibits twitching, swarming, and swimming motility of P. aeruginosa (p < 0.01). Scanning electron microscopy, fluorescent microscopy, and light microscopy showed thinner biofilms with low exopolysaccharide matrix (EPS) in the presence of PG. Moreover, the role of PG was also evaluated using molecular docking and molecular dynamics simulation to understand the interaction of PG with bacterial type-IV pili subunit, PilY1. PG showed favourable interactions and stable complex formation with type-IV pili subunit (PilY1). The present study highlights the antibiofilm properties of PG, suggesting its potential as a biofilm control flavoring compound.

Non-invasive detection of pesticide residues in freshly harvested olives using hyperspectral imaging technology

Pesticides play a crucial role in boosting the overall yield and productivity of agricultural produce by controlling pests, insects, and various plant diseases. However, excessive use of pesticides has led to contamination of food products and water bodies, as well as disruption of ecological and environmental systems. Global health authorities have set limits for pesticide residues in individual food items to ensure the availability of safe foods in the supply chain and to assist farmers in developing optimal agronomic practices for crop production. In Spain, specifically regarding olive cultivation, the Ministry of Agriculture, Fisheries, and Food establishes a safety period that farmers must observe from the application of the pesticide until the fruit is harvested. This period ensures that the batch of olives will comply with the maximum residue level allowed. This article proposes a methodology based on hyperspectral imaging to detect whether the olives have been sprayed with pesticide products and, if so, when the spraying occurred. The proposed methodology operates at the pixel level, where each pixel of the hyperspectral image is an instance. The pesticides evaluated were Diflufenican, Oxyfluorfen, Deltamethrin, λ-Cyhalothrin, and Tebuconazole. The results are promising and the success rates achieved over 80% accuracy for most pesticides in controlled laboratory conditions, with individual performance varying according to each pesticide's chemical properties and stability on the olive surface. While the results are promising, the scalability of this approach for larger and more diverse batches of olives requires validation under field conditions, where variations in environmental factors, olive variety, and ripeness may impact the detection accuracy. Furthermore, the study highlights key wavelengths around 750 nm and 550 nm as effective discriminators, suggesting potential for cost-effective, simplified imaging systems. Although hyperspectral imaging shows potential as an accessible, in-line monitoring solution for cooperative use, further analysis of implementation costs is recommended to confirm its feasibility on an industrial scale.

The Brief Case Burkholderia cepacia infection in the neonatal unit: a cautionary tale of contaminated EEG skin preparation

Burkholderia cepacia isolated from a scalp wound of a neonate prompted investigation to identify potential sources. The same organism was isolated from numerous batches of gel used to prepare skin prior to electroencephalography (EEG), prompting a recall of this product. This case highlights the potential for contamination of equipment and products used for EEG.

Ozonation as an emerging technique for inactivating microorganisms and mitigating pesticides in apple juice

ABSTRACT Microorganisms and pesticide residues are among the major health concerns associated with food products. Although ozone (O3) is currently being employed in various applications in apple processing, there are few studies investigating the effects of O3 treatments on microorganisms and pesticides in apple juice. The purpose of this study was to investigate the effects of O3 treatment on microorganisms, pesticide residues, and physicochemical properties of apple juice and to compare with that of classical pasteurization. O3 was as effective as pasteurization against E. coli and coliform bacteria; the counts of these microorganisms were below the detectable level after a treatment for 15 min. O3 was also effective in reducing the counts of total aerobic mesophilic bacteria and yeast-mold, but longer treatments were required for total inactivation. All the pesticides investigated were degraded by O3, but at different rates. Reduction rates were quite high (75–100%) for methoxyfenozide, dimethoate, and pyrimethanil, however, acetamiprid and imidacloprid were degraded by only 7.1% and 14.5%, respectively. O3 treatment caused drastic decreases in color and turbidity values, total phenolic content, and antioxidant activity of apple juice. Results revealed that the use of ozone in juice processing has potential as a technological solution to product contamination problem.

Characteristics and pollution indices of leachates from municipal solid waste landfills in Iranian metropolises and their implications for MSW management

Leachate from municipal solid waste landfills poses a significant threat to aquatic ecosystems due to poor management practices. This study evaluated thirty leachate samples from Iranian metropolises using the Leachate Pollution Index (LPI). Various parameters, including BOD₅, COD, TDS, pH, EC, heavy metals, turbidity, PAHs, phthalates, and humic acid, were analysed. The BOD₅ levels ranged from 350 to 20,000 mg/L, and the COD levels ranged from 2,000 to 90,000 mg/L. The TDS content varied between 14.7 and 67 g/L, while the turbidity ranged from 15 to 186 NTU. Heavy metals were present but within standard limits. The phthalate concentrations ranged from 6 to 150.8 mg/L, and the humic acid concentrations ranged from 135 to 2,200 mg/L. Naphthalene was the most frequent hydrocarbon detected. The LPIs were less than 30 for all the samples, with the highest in Ahvaz and the lowest in the treated samples from Tehran. This study highlights the presence of persistent organic and hazardous contaminants in Iran’s municipal landfills, emphasizing the need for effective leachate treatment and improved waste management practices. Enhanced final disposal methods, increased waste recovery, and improved solid residue separation are crucial for preventing further leachate production and environmental contamination.

Fibrous foes: First report on insidious microplastic contamination in dietary fiber supplements

Regular consumption of health supplements to balance dietary intake has gained popularity worldwide. One such supplement that has gained popularity among consumers is dietary fibers. Microplastic (MPs) contamination in various food products is being reported worldwide. However, there is a paucity of understanding of the occurrence of MPs in dietary supplements. This study addresses this gap by investigating the degree of MPs contamination in dietary fiber supplements. Nine commonly consumed (powder and gummy-based) over-the-counter dietary fiber supplements in Australia were tested in this study. Microscopic examination revealed the presence of MPs fibers and fragments in all the tested products. Further categorization showed that MPs particles were of various colours, including black, blue, red, green, and white. The order of polymer abundance was Polyamide > Polydiallyl Phthalate > polyethylene polypropylene diene > Polyurethane = Polyethylene terephthalate > Polyethylene = Ethylene acrylic acid copolymer. Among the supplements, powder-based samples had higher MPs (at the adult dosage suggested by the manufacturer) than gummy-based product. The average predicted ingestion of microplastics from these supplements (all nine samples) was 5.89 ± 2.89 particles day−1. The dietary exposure for children and adults ranged from 0.1–0.48 and 0.18–4.08 particles day−1, respectively. Based on the microplastic contamination factor (MCF), among the nine samples tested, 69.81% exhibited a moderate level, while 20.76% showed a significant level of microplastic contamination. The polymer risk index (pRi) indicates products with very high and high-risk categories. The possible sources of MPs contamination in the products were studied. To our knowledge, this is the first study to record and quantify the presence of MPs in dietary fiber supplements, which is a direct source of MPs exposure to humans via., ingestion.

Stannous Chloride-Modified Glass Substrates for Biomolecule Immobilization: Development of Label-Free Interferometric Sensor Chips for Highly Sensitive Detection of Aflatoxin B1 in Corn.

This study presents the development of stannous chloride (SnCl2)-modified glass substrates for biomolecule immobilization and their application in fabricating sensor chips for label-free interferometric biosensors. The glass modification process was optimized, identifying a 5% SnCl2 concentration, a 45 min reaction time, and a 150 °C drying temperature as conditions for efficient protein immobilization. Based on the SnCl2-modified glass substrates and label-free spectral-phase interferometry, a biosensor was developed for the detection of aflatoxin B1 (AFB1)-a highly toxic and carcinogenic contaminant in agricultural products. The biosensor realizes a competitive immunoassay of a remarkable detection limit as low as 26 pg/mL of AFB1, and a five-order dynamic range. The biosensor performance was validated using real corn flour samples contaminated with Aspergillus flavus. The proposed approach not only provides a powerful tool for AFB1 detection for food safety monitoring but also demonstrates the potential of SnCl2-modified substrates as a versatile platform for the development of next-generation biosensors.

Environmental Contaminants in Fish Products: Food Safety Issues and Remediation Strategies.

The intentional or accidental presence of environmental contaminants, such as persistent organic pollutants, metals, and microplastics, can harm the aquatic ecosystem and their living organisms, as well as consumers of seafood. This study provides an overview of marine pollution caused by various chemicals and their toxicity to both the environment and humans. In addition to regulatory limits established for some contaminants, monitoring and management policies should mandate activities such as bioremediation and the use of carbon-based composite photocatalysts to reduce or eliminate these compounds.