Homogeneous Process Research Articles

Interactions, structures, and functions of pomegranate peel reinforced starch film

This work aimed to exploit the effects of pomegranate peel (PP) addition (10 %) and homogenization process (0–12,000 rpm) on the film-forming behavior and interactions of starch film to improve its microstructures and properties. Microrheology results showed that the viscosity of the film suspension was reduced by homogenization, and the amylopectin aggregation was restricted by PP particles under film formation. This helped improve the accessibility of starch and PP, increasing the hydrogen bond energy in the composites from 18.12 to 19.35 kJ/mol and promoting the ester bonds as confirmed by the FTIR results. As a result, the starch film reinforced by PP and homogenization showed a uniform and compact microstructure with reduced water activity, increased UV-blocking capacity (from 67.46 % to 90.11 %), enhanced mechanical resistance, and reduced water vapor permeability (from 4.70 to 2.21 × 10−10·g·m−1·s−1·Pa−1). Additionally, the starch film with 10 % PP also presented superior antimicrobial properties and antioxidant activity with rates of over 86 %, providing an effective strawberry preservation function.

Hot spots of resistance: Transit centers as breeding grounds for airborne ARG-carrying bacteriophages

The presence of pathogenic bacteria and antibiotic resistance genes (ARGs) in urban air poses a significant threat to public health. While prevailing research predominantly focuses on the airborne transmission of ARGs by bacteria, the potential influence of other vectors, such as bacteriophages, is often overlooked. This study aims to investigate the characteristics of phages and ARGs in aerosols originating from hospitals, public transit centers, wastewater treatment plants, and landfill sites. The average abundance of ARGs carried by phages in the public transit centers was 8.81 ppm, which was 2 to 3 times higher than that at the other three sites. Additionally, the abundance of ARGs across different risk levels at this site was also significantly higher than at the other three sites. The assembled phage communities bearing ARGs in public transit centers are chiefly governed by homogeneous selection processes, likely influenced by human movement. Furthermore, observations at public transit sites revealed that the average abundance ratio of virulent phages to their hosts was 1.01, and the correlation coefficient between their auxiliary metabolic genes and hosts' metabolic genes was 0.59, which were 20 times and 3 times higher, respectively, than those of temperate phages. This suggests that virulent phages may enhance their survival by altering host metabolism, thereby aiding the dispersion of ARGs and bacterial resistance. These revelations furnish fresh insights into phage-mediated ARG transmission, offering scientific substantiation for strategies aimed at preventing and controlling resistance within aerosols.

Modifications of biological membranes, fat globules and liposomes promoted by cavitation processes. Consequences and applications

Cavitation-based technologies, such as ultrasound (or acoustic cavitation, AC) and hydrodynamic cavitation (HC), are gaining interest among green processing technologies due to their cost effectiveness in operation, toxic solvent use reduction, and ability to obtain superior processed products, compared to conventional methods. Both AC and HC generate bubbles, but their effects may differ and it is difficult to make comparisons as both are based on different phenomena and are subject to different operational variables. AC is one of the most used techniques in extraction and homogenization processes at the laboratory level. However, upscaling to an industrial level is hard. On the other hand, HC is based on the passage of the liquid through a constriction (orifice plate, Venturi, throttling valve), which causes an increase in liquid velocity at the expense of local pressure, forcing the pressure around the contraction below the threshold pressure that induces the formation of cavities.Some applications of cavitation technologies, such as the production of liposomes or lipid nanoparticles (LNPs) allow the generation of delivery systems for biomedical applications.Many others (inactivation of pathogenic viruses, bacteria and algae for water purification, extraction procedures, third generation of biofuel production, green extractions) are based on the disruption of lipid membranes. There are also applications aimed at the modification of membranes (like the milk fat globule) for the development of innovative products. Process parameters, such as cavitation intensity, duration and temperature define the impact of the process on the physical, chemical, and biological characteristics of the membranes. Thus, the adequate implementation of cavitation processes requires understanding of interactions and synergistic mechanisms in complex systems and of their effects on membranes at the microscopic or molecular level. In the present work, the use of cavitation technologies for the generation of LNPs or nanostructured lipid carriers, and the effects of AC and HC treatments on several types of membrane systems (liposomes, solid lipid nanoparticles, milk fat globules, algae and bacterial membranes) are discussed, focusing on the structural and chemical modifications of lipidic structures under cavitation.

Effect of laser surface melting on microstructure and hardness of Mg–4Y–2Zn–1Zr-0.6Ca alloy

To improve the performance of magnesium and enhance its mechanical properties and microstructure, a laboratory-scale Mg alloy with the composition Mg–4Y–2Zn–1Zr-0.6Ca was developed. Subsequently, the alloy was subjected to homogenization and extrusion processes to reduce casting defects and the microstructure refinement. Additionally, surface modification was performed using a pulsed Nd:YAG laser. The findings of the experiment indicate that by controlling the laser processing parameters, it was possible to attain a microstructure devoid of any defects. Moreover, an examination of the microstructure and secondary phases was conducted through the utilization of characterization techniques such as the Optical Microscope (OM), Field Emission Scanning Electron Microscope (FESEM) equipped with Energy Dispersive Spectroscopy (EDS), and X-ray Diffraction (XRD). Furthermore, the micro hardness of the samples was evaluated using the Vickers micro hardness testing method. Consequently, the microstructures of all specimens exhibited equiaxed grains, predominantly composed of (α-Mg), (Mg24Y5+α-Zr), and the W-phase (Mg3Y2Zn3). In addition, the laser-melted surface revealed a noteworthy refinement of the grain structure, with an evolution in grain morphology from the top to the bottom of the melted area. Equiaxed grains were present in the centerline, whereas columnar grains were observed in the fusion line. Additionally, as the scanning speed increased, a finer microstructure was observed. Due to the rapid heating and cooling involved in the laser surface melting (LSM) process, the secondary phase (Mg3Y2Zn3) dissolved in the microstructure, and the (Mg24Y5+α-Zr) phase was uniformly distributed throughout the LSMed area. Ultimately, the micro hardness showed a significant increase due to the dual influence of grain refinement and the homogeneous dispersion of the secondary phase.

Effects of deforestation on multitaxa community similarity in the Brazilian Atlantic Forest.

Habitat loss can lead to biotic homogenization (decrease in β diversity) or differentiation (increase in β diversity) of biological communities. However, it is unclear which of these ecological processes predominates in human-modified landscapes. We used data on vertebrates, invertebrates, and plants to quantify β diversity based on species occurrence and abundance among communities in 1367 landscapes with varying amounts of habitat (<30%, 30-60%, or >60% of forest cover) throughout the Brazilian Atlantic Forest. Decreases in habitat amount below 30% led to increased compositional similarity of vertebrate and invertebrate communities, which may indicate a process of biotic homogenization throughout the Brazilian Atlantic Forest. No pattern was detected in plant communities. We found that habitat loss was associated with a deterministic increase in faunal community similarity, which is consistent with a selected subset of species being capable of thriving in human-modified landscapes. The lack of pattern found in plants was consistent with known variation between taxa in community responses to habitat amount. Brazilian legislation requiring the preservation of 20% of Atlantic Forest native vegetation may be insufficient to prevent the biotic homogenization of faunal communities. Our results highlight the importance of preserving large amounts of habitat, providing source areas for the recolonization of deforested landscapes, and avoiding large-scale impacts of homogenization of the Brazilian Atlantic Forest.

Novel oxidant-free VUV/Fe2+/oxalate process for high-efficiency removal of norfloxacin: Oxidation performance and synergistic mechanism

Based on Vacuum UV (VUV) in situ cracking water to generate reactive oxygen species (ROS) and promote Fe2+/Fe3+ redox cycle, a novel oxidant-free VUV/Fe2+/oxalate (Ox) homogeneous oxidation process was built to efficiently degrade norfloxacin (NOR). The performance and synergistic mechanism of VUV/Fe2+/Ox for NOR degradation under near-neutral conditions were investigated. Compared with sub-processes, VUV/Fe2+/Ox obviously accelerated NOR oxidation and reduced energy consumption, and its synergistic factor of NOR removal was 2.32. Effects of operating parameters Fe2+, Ox dose and solution pH on VUV/Fe2+/Ox and their mechanisms were analyzed. Synergistic mechanisms of NOR degradation via VUV/Fe2+/Ox, such as in situ generation of H2O2, Fe2+/Fe3+ redox cycle, ROS characterization and contribution analysis, NOR degradation pathways and its toxicity changes were also investigated. Through the use of ROS fluorescence characterizations and quenching experiments, hydroxyl radical and superoxide radical were identified as primary ROS in VUV/Fe2+/Ox, and their contributions to NOR degradation were 73.17 % and 20.91 %, respectively. Besides, synergistic mechanisms of VUV/Fe2+/Ox process, which mainly included the respective roles of VUV irradiation, Fe2+ and Ox, were also recommended. Furthermore, effects of chelators and water matrices on NOR degradation via VUV/Fe2+/Ox were also researched. VUV/Fe2+/Ox process showed satisfactory NOR degradation effects in actual waters, indicating its practical application potential. The degradation of NOR by novel VUV/Fe2+/Ox process significantly reduced its environmental and health hazards.

Study on the Crashworthiness of a Battery Frame Design for an Electric Vehicle Using FEM

This paper presents an optimized method for evaluating and enhancing the crashworthiness of an electric vehicle (EV) battery frame, leveraging finite element model (FEM) simulations with minimal computational effort. The study begins by utilizing a publicly available LS-DYNA model of a conventional Toyota Camry, simplifying it to include only the structures relevant to a side pole crash scenario. The crash simulations adhere to FMVSS214 and UNR135 standards, while also extending to higher speeds of 45 km/h to evaluate performance under more severe conditions. A dummy frame with virtual mass is integrated into the model to approximate the realistic center of gravity (COG) of an EV and to facilitate visualization. Based on the side pole crash results, critical parameters are extracted to inform the development of load cases for the EV battery. The proposed battery frame, constructed from aluminum, houses a representative volume of battery cells. These cells are defined through a homogenization process derived from individual and pack of cell crash tests. The crashworthiness of the battery frame is assessed by measuring the overall intrusion along the Y-axis and the specific intrusion into the representative volume. This method not only highlights the challenges of adapting conventional vehicle platforms for EVs or for dual compatibility with both conventional and electric powertrains but also provides a framework for developing and testing battery frames independently. By creating relevant load cases derived from full vehicle crash data, this approach enables battery frames to be optimized and evaluated as standalone components, offering a method for efficient and adaptable battery frame development. This approach provides a streamlined yet effective process for optimizing the crash performance of EV battery systems within existing vehicle platforms.

Bi-directional homogenization method for the design of multi-scale mechanical metamaterials

Inverse Homogenization (IH) is a classical concept for the Topology Optimization (TO) of metamaterials. Traditional IH design methods are mainly the single-scale TO within a Representative Volume Element (RVE), suffering from challenges like design inefficiency and under-utilization of the design space. To address the problems, a Bi-Directional Homogenization (BDH) method based on the multi-scale TO principle is proposed for the design of mechanical metamaterials. The general design framework includes a forward homogenization process from the microscale to the mesoscale, and an inverse design process from the macroscale to the mesoscale. Firstly, at the microscale, the Graded Microstructures (GMs) are generated via a multi-cut level set method. Then, by varying the relative densities, the microstructure instances are sampled and the mesoscopic equivalent properties are computed using the homogenization method. After that, a spectral decomposition-based interpolation model is used to predict the relationship between the relative densities and the elastic tensors. These preparations allow for the mesoscopic optimization of the GMs distribution, and the reconstruction of the graded multi-scale metamaterial structures by using a mapping transformation on the density field. Various types of auxetic metamaterials are performed to demonstrate the effectiveness and versatility of the proposed method.

Stochastic model of seed dispersal with homogeneous and non-homogeneous Poisson processes under habitat reduction conditions.

This study presents a stochastic model of seed dispersal based on a branching random walk (BRW) framework, incorporating both homogeneous and non-homogeneous Poisson point processes (PPP). Building on the model introduced by Coletti et al. (2023), we examine the effects of habitat reduction on seed dispersal dynamics. We analyze the phase transition behavior of the BRW model under varying conditions of habitat fragmentation, focusing on how these conditions influence the critical dispersal rate. Specifically, we study a BRW on the real line with a non-homogeneous PPP driven by a log-normal density, constrained between spatial barriers. Our simulations localize the critical dispersal rate with respect to barrier positions and compare this dependence between homogeneous and non-homogeneous models.

IMPROVEMENT OF THE HOMOGENIZER HEAD DESIGN

Summary. An analysis of the equipment used for the homogenization of milk raw materials was carried out. Technologies for obtaining homogenized milk are considered. Disadvantages of existing technological processes have been identified. The areas of improvement of homogenization processes are defined and the need for the development of equipment for their implementation is substantiated. The research methods of the homogenization process are substantiated, a description of the studied raw materials is given, and the design of the homogenizer head is proposed. The influence of the geometric parameters of the modernized homogenizer head on the efficiency of the homogenization process was determined, and the optimal parameters were determined experimentally. As a result of the conducted research, it can be concluded that the homogenization process is very important for the production of pharmaceutical emulsions. The calculation and modeling of the homogenizing head of the valve homogenizer was carried out. In order to carry out two-stage homogenization, it was proposed to modernize the homogenizing head. the previous design is inferior to the modernized one in terms of homogenization of food liquids.

Impact of Thermal Energy on Water and Ethylene Glycol (50:50)-Based Rotating Hybrid Nanofluid Past A Riga Surface with Radiation, Homogeneous and Heterogeneous Reactions

Hybrid nanofluid is crucial in improving the efficiency of heat transmission in thermal engineering applications, particularly the cooling of electrical equipment, heat exchangers, fuel cells, printing machines, etc. This study sought to investigate the augmentation of heat and mass transmission by the use of a rotating hybrid nanofluid consisting of a mixture of gold and silver nanoparticles suspended in water and ethylene glycol (50:50) past a heated Riga surface with velocity slip and suction. The energy equation is constructed using nonlinear radiation and heat consumption. The impact of both homogeneous and heterogeneous processes on the hybrid nanofluid is also explored. The governing mathematical model begins with partial differential equations that are converted into ordinary differential equations using a suitable conversion technique. The results of numerical computations are recorded as graphs and tables using the bvp4c scheme in MATLAB. It is noticed that both directional velocities undergo significant negative changes as a result of enhanced values of the rotational parameter. The higher levels of the radiation parameter resulted in significant enhancements in the thermal profile. The falling behavior of the nanoparticle concentration profile is recorded against a greater quantity of both chemical reaction parameters. Based on our analysis, when the Biot number changes from 0.4 to 0.8, the most significant heat transmission gradient occurs.

Selective diesterase-like activity of bioinspired FeIIINiII and FeIIIZnII complexes and their 3-aminopropyl silica composites: A homo- and heterogeneous catalytic study

In this study, the preparation and characterization of two new mixed-valence heterodinuclear complexes [FeIIINiII(BPPAMFF)(μ-OAc)2(H2O)]ClO4 (1) and [FeIIIZnII(BPPAMFF)(μ-OAc)2(H2O)]ClO4 (2) with the bioinspired ligand 2-[(N-benzyl-N-2-pyridylmethylamine)]-4-methyl-6-[N-(2-pyridylmethyl)aminomethyl)])-4-methyl-6-formylphenol (H2BPPAMFF) are reported. These compounds were immobilized in 3-aminopropyl silica (APS) to afford composites APS-1 and APS-2 successfully. The aldehyde-containing ligand provided a reactive functional group, which could serve as a cross-linking group to bind the complexes to the directly amino-modified SiO2 surface. The complexes’ chemical integrity on the APS inorganic platform were probed by spectroscopical techniques, such as FTIR, UV–Vis and EPR. Potentiometric and spectrophotometric titrations allowed the chemical species present in solution to be rationalized, and identified which of them were potentially active in the hydrolytic cleavage of phosphodiester 2,4-BDNPP. Kinetic studies showed that FeIIINiII species (1 and APS-1) presented higher catalytic efficiency (E = kcat/KM) than FeIIIZnII species (2 and APS-2). Catalytic mechanisms were proposed based on a series of kinetic experiments, in which all the catalysts tested behaved as selective phosphodiesterases. In addition, it was also demonstrated that the hydrolase activity of the immobilized catalytic centers, APS-1 and APS-2, was better than the homogeneous processes, where second coordination sphere effects may be involved in directing and stabilizing the transition state.

Simulation of homogenization behavior of compacted bentonite containing technological voids using modified penalty-based contact model

Low-density zones generated during the bentonite blocks/voids homogenization process in the repository may serve as potentially preferential paths for radionuclide leakage. More importantly, void closure during homogenization process involves complex contact problems, where the stiffness at the contact interface typically undergoes significant fluctuations. In this work, with contact interface stiffness addressed through a step function approach, a modified penal.ty-based contact model was proposed to simulate the contact behavior involved at the gap closure stage of the bentonite/gap assemblage homogenization process. Then, unsaturated infiltration swelling tests on bentonite block (1.7 Mg/m3)/gap (width: 2 mm) assemblages were performed, and the variation of dry density at different hydrated times (0, 24, 72, 168, and 720 h) in specific areas were measured. Based on the results, time-dependent swelling pressure profiles of the assemblage were acquired, while the homogenization process was evaluated. Results reveal that after approximately 40 h of hydration, the gap is completely closed, and the radial stress condition of the compacted bentonite transits progressively from the initial free swelling into a constant volume expansion state. The swelling pressure correspondingly develops quickly to a peak value at 1.8 MPa once the hydration starts, then decreases to a valley value of 1.4 MPa at the complete gap closure, and subsequently begins to increase to the final stable value of 1.8 MPa. Further examination reveals that as hydration advances, dry density of the assemblage converges to the expected final dry density with a maximum residual inhomogeneity of about 2 %. Finally, validations demonstrate that the proposed model can accurately reproduce deformations of the assemblage during the free swelling stage, and the swelling pressure profiles. A comparative analysis was made with the previous approach of identifying gaps as highly deformable materials, revealing that the proposed model overcomes the traditional limitations associated with the separation or penetration behavior occurring between the compacted bentonite and contact boundaries during the gap closure.

DUALITY: DETECTABILITY VERSUS STABILIZABILITY IN THE STOCHASTIC CONTEXT

The aim of this paper is to extend to the stochastic framework the well known duality relation between the detectability property and the stabilizability property of a finite dimensional, linear time invariant, deterministic system. We consider continuous time linear stochastic systems having the state space representation described by a system of Itˆo differential equations with periodic coefficients possibly subject to some stochastic perturbations modeled by a standard homogeneous Markov process with a finite number of states. It will be seen that in the case when the given system is affected by jump Markov perturba tions a state space representation of the dual triple may be rigorously defined if and only if the transition probability matrix of the Markov process is double stochastic (in the sense that the sums of all elements from its each row and each column are equal 1).

SWEET-Cat: A view on the planetary mass-radius relation

Aims. SWEET-Cat (Stars With ExoplanETs Catalog) was originally introduced in 2013, and since then, the number of confirmed exoplanets has increased significantly. A crucial step for a comprehensive understanding of these new worlds is the precise and homogeneous characterization of their host stars. Methods. We used a large number of high-resolution spectra to continue the addition of new stellar parameters for planet-hosting stars in SWEET-Cat following the new detection of exoplanets listed both in the Extrasolar Planets Encyclopedia and in the NASA exoplanet archive. We obtained high-resolution spectra for a significant number of these planet-hosting stars, either observed by our team or collected through public archives. For FGK stars, the spectroscopic stellar parameters were derived for the spectra following the same homogeneous process using ARES+MOOG as for the previous SWEET-Cat releases. The stellar properties were combined with the planet properties to study possible correlations that could shed more light on the star-planet connection studies. Results. We have increased the number of stars with homogeneous parameters by 232 (~25% – from 959 to 1191). We focus on the exoplanets that have had both mass and radius determined to review the mass-radius relation, and we find results consistent with the ones previously reported in the literature. For the massive planets, we also revisit the radius anomaly, confirming a metallicity correlation for the radius anomaly already hinted at in previous results.

Homogeneous Catalysts of RedOx Processes Based on Heteropolyacid Solutions. VI: Developing a Process for Low-Temperature Oxidation of CO with Oxygen

Research on the development of a homogeneous process for low-temperature oxidation of carbon monoxide in the presence of a “platinum group metal + vanadium-containing heteropolyacid (HPA)” catalytic system has been presented. The optimal reaction conditions, ensuring the maximum rate of CO oxidation to CO2, have been determined; for this reaction the kinetic features have been established, and its mechanism has been proposed. It has been shown that homogeneous systems based on PdII complex exhibit high activity and productivity, but have low stability and operate only at pH below 1,5. The stability of the catalyst can be increased by simultaneous introduction of σ- and π-donor ligands into the system; however, it is more effective to use the PtIV complex in the presence of catalytic amounts of palladium salt at a ratio of 100/1. The transition from HPA solutions with a low content of vanadium atoms (H7PMo8V4O40) to solutions of modified compositions (H10P3Mo18V7O84) ensures an increase in the activity and productivity of the system, with the kinetics of CO oxidation being maintained. The combined homogeneous catalyst PtIV + PdII + H10P3Mo18V7O84 remains stable during multi-cycle use without reducing activity, operates without an induction period and can be used at pH range of 1,7–2,0, which simplifies the process equipment.

Graphite Made from Coal by High-Temperature Treatment: An Insight into the Nanometric Carbon Structural Evolution

Graphite made from coal will not only widen the graphite mineral resource, but also significantly improve the value of coal utilization. In this study, anthracite coal was heated in the temperature range of 500 to 2900 °C to study the size increase of nanometric graphite crystallites from anthracite to real graphite. The carbon content rapidly increases to 99.2% when heated from room temperature to 1600 °C, and then gradually increases to 100% when the treated temperature increases to 2900 °C. The FTIR results show that methyl, methylene, and aromatic hydrocarbon, preexisting in the raw anthracite, were preserved in the JZS-500 sample, but that when the treated temperature ≥ 1000 °C, these C-H bonds almost disappear. The basic structural units (nano graphitic carbon) grow into distorted columns, and the basic structural units and micro-columns re-oriented and coalesced to form local molecular oriented domains with the temperature increase from anthracite to JZS-1500. When the temperature ≥ 1600 °C, amorphous carbon, onion-like carbon, turbostratic layers, and graphitic carbon co-occur within the graphitized coals. At the sub-micron scale, carbonization is a homogenous process, whereas graphitization is a heterogenous process. The average graphite crystalline size (La, lateral extension; Lc, stacking height) rapidly increases as the treatment temperature increases from 1600 to 2300 °C. Three coal structural transformation stages were classified according to the nanometric carbon structural evolution with temperature. This study will contribute to the efficient and value-added utilization of coal to make graphite materials.

Continental-scale sediment mixing and dispersal across northern Gondwana: detrital zircon U-Pb-O-Hf isotopic evidence from the Cambro-Ordovician sandstones overlying the Arabian Shield

ABSTRACT The juvenile Neoproterozoic basement of the Arabian Shield is overlain with angular unconformity by a voluminous Cambro-Ordovician cover sequence known as the Saq Formation and Wajid Group. Provenance studies of this vast siliciclastic cover over the Saudi Arabian part of the Arabian-Nubian Shield (ANS) have to date been based solely on U-Pb zircon data and heavy minerals. We present the first combined in-situ U-Pb, δ18O and Lu-Hf isotopic data for detrital zircon from the Saq and Wajid units exposed along the northeastern margin and southern part of the Arabian Shield. U-Pb age spectra reveal prominent age peaks at ca. 0.8–0.55 Ga and 1.1–0.9 Ga with subordinate peaks at ca. 2.2–1.7 Ga and 2.7–2.5 Ga. The δ18O secular variation mirrors global compilations with Archaean zircon defining a restricted δ18O range of ca. 4.0–8.0 ‰ and younger zircon showing wide variation in δ18O up to ca. 14 ‰. The ca. 0.8–0.55 Ga age peak has the largest variation in εHf(t) with about half of all these Neoproterozoic zircon grains being juvenile (εHf(t) > 5), which are interpreted to be sourced from the juvenile terranes of the ANS. Neoproterozoic zircon with evolved εHf(t) signatures require a more distal source beyond the ANS. As extensive ca. 1.1–0.9 Ga crust is lacking in the vicinity of the ANS, the ca. 1.1–0.9 Ga age peak is interpreted to be derived from either contemporaneous orogenic belts in Central Africa or recycled from older sedimentary rocks containing these age components. Extreme variations in δ18O of post–Archaean zircon, together with the evolved εHf(t), indicate crustal thickening and increased incorporation of supracrustal material associated with collisional orogenesis. The remarkable similarities in age spectra and isotopic compositions of the Saq Formation and Wajid Group sandstones with those from other regions in northern Gondwana indicate a continental–scale homogenization and dispersion process.

Application of Response Surface Methodology to Optimize Mononitrotoluen Aquatic Removal by Photo-fenton Process

In this study, the effectiveness of pH (1-5), hydrogen peroxide concentration (5-10 mM), ion Fe2+ concentration (0,1-0,5 mM), and reaction time on treatment of mononitrotoluen by homogeneous Photo-Fenton process was evaluated. The response surface methodology (RSM) was applied to design the experiment and determine the optimal treatment. The results indicated, at optimized pH=3, C0MNT=100 mg/L, CFe2+= 0.4 mM, CH2O2= 2.5 mM, the MNT treatment efficiency reached 99.4% after 5 minutes.

Long-read genome assembly of the insect model organism Tribolium castaneum reveals spread of satellite DNA in gene-rich regions by recurrent burst events.

Eukaryotic genomes are replete with satellite DNAs (satDNAs), large stretches of tandemly repeated sequences that are mostly underrepresented in genome assemblies. Here we combined nanopore long-read sequencing with a reference-guided assembly approach to generate an improved, high-quality genome assembly, TcasONT, of the model beetle Tribolium castaneum Enriched by 45 Mb in repetitive regions, the new assembly comprises almost the entire genome sequence. We use the enhanced assembly to conduct global and in-depth analyses of abundant euchromatic satDNAs. Unexpectedly, we show the extensive spread of satDNAs in gene-rich regions, including long arrays. The sequence similarity relationships between satDNA monomers and arrays indicate a recent exchange of satDNA arrays between different chromosomes. We propose a scenario of their genome dynamics characterized by repeated bursts of satDNAs spreading through euchromatin, followed by a process of elongation and homogenization of arrays. We find that suppressed recombination on the X Chromosome has no significant effect on the spread of satDNAs but the X rather tolerates the amplification of satDNAs into longer arrays. Analyses of arrays' neighboring regions show a tendency of one satDNA to be associated with transposable-like elements. Using 2D electrophoresis followed by Southern blotting, we prove Cast satDNAs' presence in the fraction of extrachromosomal circular DNA (eccDNA). We point to two mechanisms that enable this satDNA spread to occur: transposition by transposable elements and insertion mediated by eccDNA. The presence of such a large proportion of satDNA in gene-rich regions inevitably gives rise to speculation about their possible influence on gene expression.