High-yield Process Research Articles

Formation of N ε-Carboxymethyl-Lysine and N ε-Carboxyethyl-Lysine in Pacific Oyster (Crassostrea gigas) Induced by Thermal Processing Methods.

Advanced glycation end products (AGEs) are important endogenous hazardous substances produced during the thermal processing of foods, which have attracted much attention due to the potential health risks. The current research first investigated the effect of different thermal processing methods (steaming, boiling, sous vide (SV), and sterilizing) on the formation of two typical markers of AGEs, including Nε-carboxymethyl-lysine (CML) and Nε-carboxyethyl-lysine (CEL), in Pacific oyster (Crassostrea gigas). The compositions, lipid oxidation, di-carbonyl compounds, and AGEs in 12 kinds of processed oysters were detected, and the Index values (total Z-score) were calculated. The SV treatment at 70°C caused higher processing yield and lower CEL level while sterilizing in oil at 121°C greatly resulted in the formation of CML. The Index value of SV-treated oysters was much lower than steamed, boiled, and sterilized ones. Correlation analysis showed that the CML and CEL levels were positively correlated with fat content, a* and b* value (p < 0.05), and negatively correlated with moisture content and L* value (p < 0.05). Besides, thiobarbituric acid reactive substances had a negative correlation with CML (r = −0.63, p < 0.05) while no significant correlation with CEL (p > 0.05), suggesting that lipid oxidation had a greater effect on the formation of CML but less on the formation of CEL. In summary, SV treatment at 70°C within 15 min was a recommended thermal processing method to reduce the formation of AGEs in oysters.

Perovskite quantum dot-coated YAG:Ce composites for warm white light-emitting diodes

Widespread application of YAG:Ce-based white light-emitting diodes (WLEDs) is being challenged due to the lack of red emission, leading to their poor color rendering index (Ra) and high correlated color temperature (CCT). Perovskite quantum dots (QDs), presenting high photoluminescence quantum yield and simple fabrication process have been recognized as promising red-emitting component for warm WLEDs. However, conventional method by mechanically mixing QDs with YAG:Ce always results in undesirable dispersion due to their huge size difference. Moreover, the two components display different sinking behavior in silicone, which would further deteriorate the color uniformity of WLED. To address these issues, in this study, red-emitting MAPbBr3-xIx QDs-coated YAG:Ce (A-YAG@QDs) composites were successfully prepared to further fabricate WLED with compensated red emission. The chemical coordination interactions between QDs and functionalized YAG:Ce make the two components behave as a whole, which effectively avoids the dispersion and sinking-related issues. The WLED, which combined blue LED chip and A-YAG@QDs composite, exhibited warm white light with low CCT of 4474 K, high Ra of 90.1 and high value of saturated red color component R9 as 95. This study demonstrated a promising strategy to prepare QDs-coated phosphor composites for the generation of warm white light for indoor lighting application.

Development of 3D Wafer Level Hermetic Packaging with Through Glass Vias (TGVs) and Transient Liquid Phase Bonding Technology for RF Filter.

The development of 5G mobile communication created the need for high-frequency communication systems, which require vast quantities of radio frequency (RF) filters with a high-quality factor (Q) and low inband losses. In this study, the packaging of an RF filter with a through-glass via (TGV) interposer was designed and fabricated using a three-dimensional wafer-level package (3D WLP). TGV fabrication is a high-yielding process, which can produce high precision vias without masking and lithography and reduce the manufacturing cost compared with the through silicon via (TSV) solution. The glass interposer capping wafer contains Cu-filled TGV, a metal redistribution layer (RDL), and the bonding layer. The RF filter substrate with Au bump is bonded to the capping wafer based on Au-Sn transient liquid phase (TLP) bonding at 280 °C with a 40 kN (approximately 6.5 MPa) bonding force. Experimental results show that shear strengths of approx. 54.5 MPa can be obtained, higher than the standard requirement (~6 MPa). In addition, a comparison of the electrical performance of the RF filter package after the pre-conditional level three (Pre-Con L3) and unbiased highly accelerated stress (uHAST) tests showed no difference in insertion attenuation across the passband (<0.2 dB, standard value: <1 dB). The final packages passed the reliability tests in the field of consumer electronics. The proposed RF filter WLP achieves high performance, low cost, and superior reliability.

Simple reaction to prepare a heat-resistant and insensitive explosive (2-nitro-[1,2,4]triazolo[1,5-a][1,3,5]triazine-5,7-diamine) and its derivatives

• An energetic molecule (NTTD) was obtained by one-step reaction with high yield. • NTTD can act as a heat-resistant and insensitive explosive. • The reaction has many advantages, which are conducive to large-scale preparation. • The derivatives of NTTD were synthesized through simple derivatization reactions. Low cost, high yield, good safety, and simple synthesis process are the problems that must be solved in the engineering preparation of energetic compounds, even beyond the pursuit of high energy level to some extent. In this work, a novel 5,6-fused energetic molecule 2-nitro-[1,2,4]triazolo[1,5- a ][1,3,5] triazine-5,7-diamine (NTTD) was designed and easily obtained. By the introduction of oxidants (HNO 3 and HClO 4 ) and high-energy groups (–NHNO 2 ), the derivatives of NTTD were prepared, some of which exhibited good comprehensive properties. Importantly, NTTD features excellent thermostability and low mechanical sensitivities. The whole preparation process has advantages of simple operation, short time, cheap and simple reagents, good repeatability, and high yield, which are conducive to large-scale preparation, indicating that NTTD can be a potential candidate of heat-resistant and insensitive explosives.

Rapid, Multianalyte Detection of Opioid Metabolites in Wastewater.

By monitoring opioid metabolites, wastewater-based epidemiology (WBE) could be an excellent tool for real-time information on the consumption of illicit drugs. A key limitation of WBE is the reliance on costly laboratory-based techniques that require substantial infrastructure and trained personnel, resulting in long turnaround times. Here, we present an aptamer-based graphene field effect transistor (AptG-FET) platform for simultaneous detection of three different opioid metabolites. This platform provides a reliable, rapid, and inexpensive method for quantitative analysis of opioid metabolites in wastewater. The platform delivers a limit of detection 2-3 orders of magnitude lower than previous reports, but in line with the concentration range (pg/mL to ng/mL) of these opioid metabolites present in real samples. To enable multianalyte detection, we developed a facile, reproducible, and high-yield fabrication process producing 20 G-FETs with integrated side gate platinum (Pt) electrodes on a single chip. Our devices achieved the selective multianalyte detection of three different metabolites: noroxycodone (NX), 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), and norfentanyl (NF) in wastewater diluted 20× in buffer.

Optical Fiber, Nanomaterial, and THz-Metasurface-Mediated Nano-Biosensors: A Review.

The increasing use of nanomaterials and scalable, high-yield nanofabrication process are revolutionizing the development of novel biosensors. Over the past decades, researches on nanotechnology-mediated biosensing have been on the forefront due to their potential application in healthcare, pharmaceutical, cell diagnosis, drug delivery, and water and air quality monitoring. The advancement of nanoscale science relies on a better understanding of theory, manufacturing and fabrication practices, and the application specific methods. The topology and tunable properties of nanoparticles, a part of nanoscale science, can be changed by different manufacturing processes, which separate them from their bulk counterparts. In the recent past, different nanostructures, such as nanosphere, nanorods, nanofiber, core–shell nanoparticles, nanotubes, and thin films, have been exploited to enhance the detectability of labelled or label-free biological molecules with a high accuracy. Furthermore, these engineered-materials-associated transducing devices, e.g., optical waveguides and metasurface-based scattering media, widened the horizon of biosensors over a broad wavelength range from deep-ultraviolet to far-infrared. This review provides a comprehensive overview of the major scientific achievements in nano-biosensors based on optical fiber, nanomaterials and terahertz-domain metasurface-based refractometric, labelled and label-free nano-biosensors.

Upcycling Cotton Textile Waste into Bio-Based Building Blocks Through an Environmentally Friendly and High-Yield Conversion Process

Upcycling Cotton Textile Waste into Bio-Based Building Blocks Through an Environmentally Friendly and High-Yield Conversion Process

Production of &amp;lsquo;kedondong&amp;rsquo; (Spondias cytherea Sonnerat) powder as affected by different drying methods [pdf

BACKGROUND ‘Kedondong’ fruit is regarded as an exotic fruit that is gaining popularity due to its deliciousness and pleasant flavour. However, this fruit has a short shelf life, leading to problems with postharvest loss. In order to prevent losses, the fruit could be produced as a value-added product. In this study, the ‘kedondong’ fruit was preserved by drying into powder using different drying methods. METHODS The kedondong powder was dried using five methods: convection oven drying, vacuum drying, spray drying, drum drying and freeze drying. The physical properties, flowability and DPPH radical scavenging ability of dried kedondong powder were examined. RESULTS Spray-dried powder provided the significantly (p ≤ 0.05) highest process yield, which was 54.93%. All the powder produced had a low moisture content (3.03 to 5.66%) and water activity (0.19–0.37). Visually, whitish and fine powders were observed on spray-dried and freeze-dried samples, while convection oven-dried and vacuum-dried powder appeared yellowish and coarse. The pH of the reconstituted powders varied from 2.71 to 2.83, where drum-dried powder was the most acidic. Spray-dried powder showed the highest wettability and shortest dissolution time, which was 172.65 s and 10.55 s, respectively. With the exception of drum-dried powder, all the dried powders were classified as non-caking powders. The bulk and tapped density of the powders ranged from 0.32 to 0.70 g/mL and 0.38 to 0.86 g/mL, respectively. Vacuum-dried powder had very good flowability, convection oven-dried and drum-dried powder had good flowability, while spray-dried and drum-dried powder had fair flowability. Antioxidant assay showed that freeze-dried powder exhibited the highest free radical scavenging activity (IC50 = 701.29 μg/mL). CONCLUSIONS This study indicates that spray-dried kedondong powder has great potential in the food industry due to its high process yield and better powder quality. Meanwhile, freeze drying best preserved the antioxidant properties of the powder, which could potentially be used as a functional ingredient as a result. This study is important for the fruit processing industry as it offers an alternative for the farmer to produce kedondong fruit powder because the fruit has a short shelf life. Converting the fruit into powder can diversify the resulting produce into different applications, such as fruit juice, beverages, jam and other food products.

Production of &lsquo;kedondong&rsquo; (Spondias cytherea Sonnerat) powder as affected by different drying methods.

&lsquo;Kedondong&rsquo; fruit is regarded as an exotic fruit that is gaining popularity due to its deliciousness and pleasant flavour. However, this fruit has a short shelf life, leading to problems with postharvest loss. In order to prevent losses, the fruit could be produced as a value-added product. In this study, the &lsquo;kedondong&rsquo; fruit was preserved by drying into powder using different drying methods. The kedondong powder was dried using five methods: convection oven drying, vacuum drying, spray drying, drum drying and freeze drying. The physical properties, flowability and DPPH radical scavenging ability of dried kedondong powder were examined. Spray-dried powder provided the significantly (p &le; 0.05) highest process yield, which was 54.93%. All the powder produced had a low moisture content (3.03 to 5.66%) and water activity (0.19&ndash;0.37). Visually, whitish and fine powders were observed on spray-dried and freeze-dried samples, while convection oven-dried and vacuum-dried powder appeared yellowish and coarse. The pH of the reconstituted powders varied from 2.71 to 2.83, where drum-dried powder was the most acidic. Spray-dried powder showed the highest wettability and shortest dissolution time, which was 172.65 s and 10.55 s, respectively. With the exception of drum-dried powder, all the dried powders were classified as non-caking powders. The bulk and tapped density of the powders ranged from 0.32 to 0.70 g/mL and 0.38 to 0.86 g/mL, respectively. Vacuum-dried powder had very good flowability, convection oven-dried and drum-dried powder had good flowability, while spray-dried and drum-dried powder had fair flowability. Antioxidant assay showed that freeze-dried powder exhibited the highest free radical scavenging activity (IC50 = 701.29 &mu;g/mL). This study indicates that spray-dried kedondong powder has great potential in the food industry due to its high process yield and better powder quality. Meanwhile, freeze drying best preserved the antioxidant properties of the powder, which could potentially be used as a functional ingredient as a result. This study is important for the fruit processing industry as it offers an alternative for the farmer to produce kedondong fruit powder because the fruit has a short shelf life. Converting the fruit into powder can diversify the resulting produce into different applications, such as fruit juice, beverages, jam and other food products.

Promoter-Driven Overexpression in Chromobacterium vaccinii Facilitates Access to FR900359 and Yields Novel Low Abundance Analogs.

Access to the cyclic depsipeptide FR900359 (FR), a selective Gq/11 protein inhibitor of high pharmacological interest and a potential lead molecule for targeted therapy of cancers with oncogenic GNAQ or GNA11 mutations (encoding Gq and G11 respectively), has been challenging ever since its initial discovery more than three decades ago. The recent discovery of Chromobacterium vaccinii as a cultivable FR producer enables the development of approaches leading to a high-yielding, scalable and sustainable biotechnological process for production of FR, thereby removing this bottleneck. Here we characterize different promoters in exchange of the native promoter of the FR assembly line, resulting in an overexpression mutant with significantly increased production of FR. Thereby, the isolation and structure elucidation of novel FR analogs of low abundance is enabled. Further, we explore the antiproliferative activities of fifteen chromodepsins against uveal melanoma cell lines harboring Gq/11 mutations and characterize the major metabolite of FR formed in plasma.

Trio exome sequencing is highly relevant in prenatal diagnostics.

ObjectiveAbout 3% of newborns show malformations, with about 20% of the affected having genetic causes. Clarification of genetic diseases in postnatal diagnostics was significantly improved with high‐throughput sequencing, in particular through whole exome sequencing covering all protein‐coding regions. Here, we aim to extend the use of this technology to prenatal diagnostics.MethodBetween 07/2018 and 10/2020, 500 pregnancies with fetal ultrasound abnormalities were analyzed after genetic counseling as part of prenatal diagnostics using WES of the fetus and parents.ResultsMolecular genetic findings could explain ultrasound abnormalities in 38% of affected fetuses. In 47% of these, disease‐causing de novo variants were found. Pathogenic variants in genes with autosomal recessive or X‐linked inheritance were detected in more than one‐third (70/189 = 37%). The latter are associated with increased probability of recurrence, making their detection important for further pregnancies. Average time from sample receipt to report was 12 days in the recent cases.ConclusionTrio exome sequencing is a useful addition to prenatal diagnostics due to its high diagnostic yield and short processing time (comparable to chromosome analysis). It covers a wide spectrum of genetic changes. Comprehensive interdisciplinary counseling before and after diagnostics is indispensable.

Fuelling the future of sustainable sugar fermentation across generations.

Biomanufacturing in the form of industrial sugar fermentation is moving beyond pharmaceuticals and biofuels into chemicals, materials, and food ingredients. As the production scale of these increasingly consumer-facing applications expands over the next decades, considerations regarding the environmental impact of the renewable biomass feedstocks used to extract fermentable sugars will become more important. Sugars derived from first-generation biomass in the form of, for example, corn and sugarcane are easily accessible and support high-yield fermentation processes, but are associated with the environmental impacts of industrial agriculture, land use, and competition with other applications in food and feed. Fermentable sugars can also be extracted from second- and third-generation feedstocks in the form of lignocellulose and macroalgae, respectively, potentially overcoming some of these concerns. Doing so, however, comes with various challenges, including the need for more extensive pretreatment processes and the fermentation of mixed and unconventional sugars. In this review, we provide a broad overview of these three generations of biomass feedstocks, outlining their challenges and prospects for fuelling the industrial fermentation industry throughout the 21st century.

Metabolic Engineering of &lt;i&gt;Escherichia coli&lt;/i&gt; for Production of Ectoine

Ectoine, a cyclic amino acid derivative is produced by some moderately halophilic microorganisms. It is widely used in the field of medicine and cosmetics; however its high cost, low yield and complicated production process restrict its use in an industry. The present study was carried out to construct an engineered Escherichia coli for the heterologous ectoine production. Firstly, the ectoine synthesis cluster (ectABC genes) from Halomonas elongata were introduced into E. coli for the ectoine production in heterologous system. Secondly, the effect of deletion for lysA and thrA on producing ectoine was investigated. Thirdly, the bioconversion conditions for synthesizing ectoine were optimized. In this study, nine strains of E.coli BW25113 were constructed, and an engineering strain ABC (∆lysA)/BW25113 with ectoine synthesis ability was screened out. An improved yield of ectoine was obtained with optimized conditions like 100 mmol/L sodium aspartate, 100 mmol/L glycerol, 80 mmol/L glucose and 120 mmol/L KCl at temperature 30°C, and the pH 7.0. A yield 2.08 g/L of ectoine was obtained. After preliminary optimization, the ABC (∆lysA)/BW25113 catalytic synthesis of ectoine increased by 2 times, indicating that optimized reaction conditions can increase the yield of ectoine. Here, we successfully constructed an engineered Escherichia coli for the heterologous production of ectoine. Our study herein provides a feasible and valuable biosynthesis pathway of ectoine with a potential for large-scale industrial production using simple and cheap feedstocks.

Epitope-coated polymer particles elicit neutralising antibodies against Plasmodium falciparum sporozoites

The current Malaria RTS,S vaccine is based on virus-like particles (VLPs) comprising the NANP repetitive epitopes from the cicumsporozoite protein (CSP) of Plasmodium falciparum. This vaccine has limited efficacy, only preventing severe disease in about 30% of vaccinated individuals. A more efficacious vaccine is urgently needed to combat malaria. Here we developed a particulate malaria vaccine based on the same CSP epitopes but using biopolymer particles (BPs) as an antigen carrier system. Specific B- and T-cell epitope-coated BPs were assembled in vivo inside an engineered endotoxin-free mutant of Escherichia coli. A high-yield production process leading to ~27% BP vaccine weight over biomass was established. The epitope-coated BPs were purified and their composition, i.e., the polymer core and epitope identity, was confirmed. Epitope-coated BPs were used alongside soluble peptide epitopes and empty BPs to vaccinate sheep. Epitope-coated BPs showed enhanced immunogenicity by inducing anti-NANP antibody titre of EC50 > 150,000 that were at least 20 times higher than induced by the soluble peptides. We concluded that the additional T-cell epitope was not required as it did not enhance immunogenicity when compared with the B-cell epitope-coated BPs. Antibodies specifically bound to the surface of Plasmodium falciparum sporozoites and efficiently inhibited sporozoite motility and traversal of human hepatocytes. This study demonstrated the utility of biologically self-assembled epitope-coated BPs as an epitope carrier for inclusion in next-generation malaria vaccines.

Phase II exponential charts for monitoring time between events data: performance analysis using exact conditional average time to signal distribution

In this paper, we study the performance properties of the phase II exponential chart with an unknown in-control (IC) rate parameter, used to monitor high-yield processes. The average time to signal (ATS) is used as the chart performance criterion instead of the usual average run length (ARL). Based on the IC conditional ATS (CATS) distribution, we examine the properties of both the equal-tailed and the ATS-unbiased exponential charts with estimated control limits and adjust the limits so that a nominal IC ATS performance is achieved. Two perspectives are investigated: the unconditional, under which the average of IC CATS distribution is set equal to a nominal ATS and, the conditional so that the IC CATS is set to or exceed a nominal ATS with a high probability. It is shown that the ATS-unbiased exponential chart under the conditional perspective has a better IC performance.

The bis(Biphenyl)phosphorus Fragment in Trivalent and Tetravalent P-Environments

Diaryl substituted phosphorus (III) compounds are commonly used motifs in synthesis. Although the basic synthetic routes to these molecules starting from PCl3 are well reported, sterically hindered aryl substituents can be difficult to introduce, especially if the P atom is in ortho position to another group. This work explores the chemistry of the bis(biphenyl)phosphorus(III) fragment. As third substituents, H, M, Cl, NR2, two group 14 element substituents and also Li were introduced in high-yielding processes offering a wide chemical variety of the bis(biphenyl) phosphine motif. In addition, also a tetravalent phosphine borane adduct was isolated. All structures were thoroughly investigated by heteronuclear NMR spectroscopic analysis. Furthermore, the reaction conditions are discussed in connection with the structures and four crystal structures of the aminophosphine, phosphine, phosphine borane and phosphide are provided. The latter crystallized as a dimer with a unique planar P2Li2 ring, which is stabilized by the non-covalent C⋯Li interaction arising from the biphenyl motif and represents a rare example of a donor-free planar P2Li2 ring.

Systematic Study of the Effects of High Shear Granulation Parameters on Process Yield, Granule Size, and Shape by Dynamic Image Analysis.

The aim of the work was to analyze the influence of process parameters of high shear granulation on the process yield and on the morphology of granules on the basis of dynamic image analysis. The amount of added granulation liquid had a significant effect on all monitored granulometric parameters and caused significant changes in the yield of the process. In regard of the shape, the most spherical granules with the smoothest surface were formed at a liquid to solid ratio of ≈1. The smallest granules were formed at an impeller speed of 700 rpm, but the granules formed at 500 rpm showed both the most desirable shape and the highest process yield. Variation in the shape factors relied not only on the process parameters, but also on the area equivalent diameter of the individual granules in the batch. A linear relationship was found between the amount of granulation liquid and the compressibility of the granules. Using response surface methodology, models for predicting the size of granules and process yield related to the amount of added liquid and the impeller speed were generated, on the basis of which the size of granules and yield can be determined with great accuracy.

Open hardware microsecond dispersive transient absorption spectrometer for linear optical response

An open hardware design and implementation for a transient absorption spectrometer are presented that has microsecond time resolution and measures full difference spectra in the visible spectral region from 380 to 750 nm. The instrument has been designed to allow transient absorption spectroscopy measurements of either low or high quantum yield processes by combining intense sub-microsecond excitation flashes using a xenon lamp together with stroboscopic non-actinic white light probing using LED sources driven under high pulsed current from a capacitor bank. The instrument is sensitive to resolve 0.15 mOD flash-induced differences within 1000 measurements at 20 Hz repetition rate using an inexpensive CCD sensor with 200 μm pixel dimension, 40 K electrons full well capacity and a dynamic range of 1800. The excitation flash has 230 ns pulse duration and the 2 mJ flash energy allows spectral filtering while retaining high power density with focussing to generate mOD signals in the 10–4–10–1 ΔOD range. We present the full electronics design and construction of the flash and probe sources, the optics as well as the timing electronics and CCD spectrometer operation and modification for internal signal referencing. The performance characterisation and example measurements are demonstrated using microsecond TAS of Congo red dye, as an example of a low quantum yield photoreaction at 2% with up to 78% of molecules excited. The instrument is fully open hardware and combines inexpensive selection of commercial components, optics and electronics and allows linear response measurements of photoinduced reactions for the purpose of accurate global analysis of chemical dynamics.Graphical abstract

Influence of temperature and time of exposure to temperature on the yield of the obtaining process of ethanolic and hydroethanolic extracts from Syzygium malaccense (L.) Merr. &amp; L. M. Perry leaves

The present study aimed to obtain alcoholic and hydroalcoholic (70% v/v) extract of Syzygium malaccense (L.) Merr. &amp; L. M. Perry (red jambo fruit tree) leaves and investigated the influence of temperature and time of exposure to temperature on the extraction yield. It was applied a 2x2 factorial design, considering temperature and time of exposure as independent variables with two levels each: 30 or 60 °C for temperature and 60 or 120 minutes for time. For both ethanolic and hydroethanolic extraction, higher process yields were observed at higher temperature and time (24.26% and 19.00%, respectively), however, no significant difference was noticed when increasing the time of exposure to temperature.

H-BN nanosheets obtained by mechanochemical processes and its application in lamellar hybrid with graphene oxide

Nowadays, hexagonal boron nitride nanosheets (h-BNNS) have shown promising results among 2D nanomaterials. A great effort has been made in recent years to obtain h-BNNS with a high-yield process to enable its large-scale application in industrial plants. In this work, we developed a mechanochemical method for obtaining h-BN nanosheets assisted by NaOH aqueous solution as process aid and aimed the ideal balance between yield, quality and process sustainability. Images obtained by transmission electron microscope suggested a great exfoliation of the h-BNNS in the range of 12–38 layers observed for well dispersed nanosheets. The macroscopic stability study, the polydispersity index, hydrodynamic diameter, and Zeta potential measurements suggested that material prepared in autoclave and ball milling followed by tip sonication process at 40 °C (h-BNNS-T40) could be considered the most promising material. The process used in this case reached a yield of about 37% of nanosheets with an optimal balance between quality and practicality. A hybrid lamellar material was also prepared by drop-casting and dip-coating techniques. An increase on thermal stability in oxidizing atmosphere was observed with respect to the pure graphene oxide (GO). Fourier transformation infrared spectroscopy and RAMAN suggested the presence of chemical interactions between h-BNNS and GO in the hybrid. This fact supports the interest of extending the study of this hybrid (which has an easy preparation method) to further explore its applicability.