A general survey of the literature pertaining to the relation of algae to free atmospheric nitrogen reveals the fact that comparatively few forms have been experimented with under conditions which render the conclusions reached free from objection. The principal fault which may be found with most of the work done is that the experiments were carried out with impure cultures. Representatives from not more than four or five genera of green alga have thus far been studied in pure culture, and while the general conclusion reached is that these forms are unable to fix free atmospheric nitrogen either in the presence or in the absence of combined nitrogen and energyfurnishing materials, it is by no means certain that forms do not exist which, under one or all of these conditions, are able to utilize elementary nitrogen. This thought is especially justified when the small number of free-nitrogen-fixing species among the bacteria is considered. In the present investigation, therefore, an attempt has been made to extend the observations over a greater variety of forms in pure culture, -understanding by the latter a single species of alga free from all other organisms.
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