Preservation Techniques Research Articles

Heat resistance of Listeria monocytogenes persistent cells following osmotic stress in cooked pork sausages

SummaryPersistent bacteria are subset of cells capable of surviving bactericidal treatments longer. They originate in response to environmental stressors and show a longer lag phase compared to the rest of the population they are part of. Impact of persistent cells on food safety and role of common food preservation techniques in their formation are still unclear. This study aims to investigate whether osmotic stress induces formation of persistent cells in Listeria monocytogenes capable of resisting longer during cooking cycle of pork sausages. Lag‐phase duration at single‐cell level of a strain grown in 6%‐NaCl broth using image processing software was first investigated. Then, trend of the strain inactivation curve was evaluated in experimentally contaminated pork sausages cooked at 65 °C for 11 min. Results showed that salt triggers the formation of persistent cells characterised by a longer lag phase and increased heat resistance, posing a concern especially for ready‐to‐eat foods.

Posterior Release, Reduction, and Intra-Articular Fusion for Irreducible Type III Atlantoaxial Rotary Fixation.

For irreducible atlantoaxial rotary fixation (AARF), anterior or posterior release was often needed before posterior reduction and fusion. Anterior atlantoaxial joint release has potential complications such as retropharyngeal abscess, persistent hoarseness, and infection. This study aims to assess the efficacy of posterior release, reduction, and intra-articular fusion without resecting the C2 nerve root on irreducible type III AARF. The data of 9 pediatric patients diagnosed with AARF who underwent posterior atlantoaxial release, reduction, and intra-articular fusion without resecting the C2 nerve root were retrospectively reviewed. Japanese Orthopaedic Association scores and Visual Analog Scale for Neck Pain were used to assess outcomes. The preoperative and follow-up assessments of atlantodens interval (ADI) were documented to evaluate the reduction of atlantoaxial joint. The patient demographics, surgery time, blood loss, bone fusion time, follow-up period, and surgery-related complications were meticulously documented. The mean follow-up duration was 35.1 ± 11.5 months. Complete reduction was achieved in 8 patients, while one patient did not achieve complete reduction. The ADI decreased significantly from 8.7 ± 2.2 mm before surgery to 2.1 ± 1.3 mm at the final follow-up. All patients demonstrated successful bone fusion, with an average fusion period of 3.7 ± 1.3 months. The Visual Analog Scale for Neck Pain at the final follow-up exhibited a significant decrease compared with preoperative values (P < .05), while no significant difference was observed in Japanese Orthopaedic Association scores. There were no complications related to surgery. Posterior atlantoaxial release, reduction, and intra-articular fusion with a C2 nerve root preservation technique is effective in the treatment of irreducible type III AARF.

Mitigating Cold Ischemic Injury: HTK, UW and IGL-2 Solution's Role in Enhancing Antioxidant Defence and Reducing Inflammation in Steatotic Livers.

Liver transplantation remains the only definitive treatment for end-stage liver diseases. However, the increasing prevalence of fatty liver disease among potential donors exacerbates the shortage of suitable organs. This study evaluates the efficacy of the preservation solution Institut Georges Lopez-2 (IGL-2) compared to Histidine-Tryptophan-Ketoglutarate (HTK) and University of Wisconsin (UW) preservation solutions in mitigating ischemia-reperfusion injury (IRI) in steatotic livers. Using Zucker Obese rat livers, we assessed the impact of 24-h static cold storage (SCS) with each solution on transaminase release, glutathione redox balance, antioxidant enzyme activity, lipoperoxidation, and inflammation markers. IGL-2 and UW solutions demonstrated reduced transaminase and lactate levels compared to HTK, indicating better preservation of liver integrity. IGL-2 maintained a higher reduced glutathione/oxidized glutathione (GSH/GSSG) ratio, suggesting more effective management of oxidative stress. Antioxidant enzyme activities catalase, superoxide dismutase, and glutathione peroxidase (CAT, SOD, GPX) were higher in IGL-2 preserved livers, contributing to decreased oxidative damage. Lipid peroxidation markers and inflammatory markers were lower in IGL-2 than in HTK, indicating reduced oxidative stress and inflammation. Additionally, improved mitochondrial function was observed in the IGL-2 group, correlating with reduced reactive oxygen species (ROS) production and lipid peroxidation. These findings suggest that IGL-2 offers superior preservation of liver viability, reduces oxidative stress, and minimizes inflammation compared to HTK and UW solutions. By maintaining a higher ratio of reduced glutathione and antioxidant enzyme activity, IGL-2 effectively mitigates the harmful effects of ischemia-reperfusion injury. The reduced lipid peroxidation and inflammation in the IGL-2 group further underscore its potential in improving liver transplant outcomes. These results highlight the importance of optimizing preservation solutions to enhance the viability and functionality of donor organs, potentially expanding the donor pool and improving the success rates of liver transplantation. Future research should focus on refining preservation techniques and exploring additional protective agents to further improve organ preservation and transplant outcomes.

Enhancing diagnostic precision in thyroid nodule assessment: evaluating the efficacy of a novel cell preservation technique in fine-needle aspiration cytology.

This study aimed to evaluate the effectiveness of thyroid fine needle aspiration cytology (FNAC) using a novel-cell preserving matrix called Cytomatrix in improving diagnostic accuracy for thyroid nodules. Fifty patients undergoing thyroidectomy were enrolled and FNAC was performed on the excised thyroid glands, with the collected sample being placed on the Cytomatrix. The results were compared with histopathological analysis, and diagnostic performance was assessed statistically. Cytomatrix demonstrated an accuracy of 96%, sensitivity of 84.61%, and specificity of 100%. Concordance between cytological and histopathological findings highlighted Cytomatrix's potential to enhance thyroid FNAC accuracy. FNAC using Cytomatrix shows promise in improving diagnostic accuracy for thyroid nodules. Its application, marked by faster processing and efficient resource utilization, coupled with the preservation of cellular architecture, holds considerable potential in enhancing cytological diagnosis, thus optimizing patient management strategies.

Effect of Poor Post-Slaughter Handling on Physicochemical and Microbial Quality of Fresh Broiler in Bangladesh

The slaughtering method and post-handling play an important role in meat processing. Countries followed different slaughtering methods, post-handling, and preservation techniques for post-slaughtered meat. Stress-oriented slaughtering, unhygienic handling, processing, and improper preservation are triggering factors of rapid oxidation and microbial proliferation in meat during storage. Bangladesh is a Muslim-oriented country but many vendors do not follow the halal slaughtering, and handling of meat in an unhygienic way due to a lack of food safety knowledge. Thus, the study aims to assess the effect of poor post-slaughtering handling practices on the physiochemical properties which include moisture, ash, protein content, fat content, drip loss, cooking loss, water holding capacity, pH, fat oxidation, color, heme iron, mineral content, and microbial qualities of fresh samples by total plate count during 0 days, 15 days and 30 days frozen storage at -18ºC. The live broiler was purchased randomly and slaughtered instantly from different slaughter shops in Jashore City, Bangladesh. The laboratory analysis was performed following the standard method of meat sample analysis. The outcome of this study revealed that heme iron content, minerals like Ca, Fe, Zn, and lightness values decreased significantly (p &lt; 0.05) during 30 days of storage at -18 ºC. Post-handling had no significant effect (p &gt; 0.05) on protein and ash content at 0 and 15 days of storage but slowly decreased with increasing storage time. The continuous increase in pH, cooking loss, and TBARS value indicate rises in lipid oxidation noticed in all samples, especially during 16-30 days of frozen storage (p &lt; 0.05). The total viable count significantly raised (p &lt; 0.05) and reached the highest value of 7.27 log10 CFU/g and 7.44 log10 CFU/g respectively after 15 days and 30 days of frozen storage compared to fresh condition. Bacteriologically meat samples were of very poor quality during storage compared to fresh ones.

Narrowing the Nasal Roof with Paramedial Osteotomy in Preservation Rhinoplasty.

Rhinoplasty is frequently performed in aesthetic and plastic surgery, with hump deformity being common in Western populations. Osteotomies play a crucial role in managing this, especially for dorsal hump reduction in closed rhinoplasty. Despite advancements, treatments for simultaneous nasal hump deformity and wide nasal roof are inadequate in preservation rhinoplasty. This study introduces a technique merging push-down and let-down methods with paramedial osteotomy for these cases. A retrospective review was conducted on patients undergoing push-down or let-down rhinoplasty, identifying 68 individuals (51 females and 17 males) with both nasal hump deformity and wide nasal roof. They were evaluated using the Rhinoplasty Outcome Evaluation questionnaire before surgery and 1-year post-operation. Follow-up ranged from 12 to 24months, with significant improvement in patient satisfaction. The median Rhinoplasty Outcome Evaluation score rose from 48.5 pre-surgery to 91.5 post-surgery (p < 0.001), indicating remarkable patient satisfaction in 97.3% of cases. This research is to demonstrate narrowing the nasal roof in patients who had nasal hump deformity and wide nasal roof with a combination of dorsal preservation techniques and osteotomy. Surgeons experienced in push-down or let-down techniques can apply this combination technique to obtain better aesthetic outcomes in patients with a wide nasal roof. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Hurdle Concept and The Role of Lactic Acid Bacteria

The hurdle idea is a food preservation technique that includes building up a number of barriers to prevent microbes from growing and prolong the shelf life of perishable goods. The idea of using lactic acid bacteria (LAB), which are essential for food preservation, is one that is significant. Through a variety of processes, including fermentation, competitive exclusion, metabolite generation, biofilm formation, nutritional competition, and metabolic activity, LAB support the hurdle concept. Lactic acid bacteria (LAB) ferment glucose to produce lactic acid, which results in an acidic environment that prevents infections and spoiling germs from growing. Additionally, LAB generates antimicrobial substances such as hydrogen peroxide, organic acids, and bacteriocins, which work by competitively excluding harmful germs from growing. Furthermore, metabolites of lactic acid bacteria (LAB) such acetic acid, diacetyl, and carbon dioxide enhance the flavor of fermented foods and serve as organic preservatives. Moreover, LAB have the ability to create biofilms that shield them from antimicrobial agents and environmental stressors, increasing their survival and persistence in food matrices. By means of nutritional competition, LAB outcompetes pathogenic or spoilage bacteria in the consumption of available resources, hence restricting their growth. In addition, LAB produces enzymes and antibacterial substances, among other metabolic activities that further impede the growth of infections.

Revisiting the external urethral sphincter: new anatomical insights from a human cadaver study.

The external urethral sphincter (EUS) is critical for urinary continence, but its complex anatomy is not fully understood, complicating its preservation during prostate surgeries. This study aims to elucidate the anatomy and development of the EUS to enhance surgical techniques for continence preservation. The study consisted of a postmortem examination of three male cadavers, aged 52, 64, and 60, with intact urogenital systems. Specimens including the prostate and EUS were dissected, fixed in formalin, and stained with Hematoxylin-Eosin for microscopic analysis. Histological assessments focused on the muscle composition and structure of the EUS and prostate. Macroscopic examination revealed symmetrical prostates without pathologies. Histologically, the anterior prostate lacked tubuloalveolar glands, showing striated muscle fibers from the external urethral sphincter extending into the prostate and prostatic urethra. Reduced glandular structure and prevalent smooth muscle were noted. This intricate integration of striated muscle fibers at the EUS-prostate interface underscores the anatomical complexity vital for surgical preservation of urinary continence. Our study reveals a complex EUS-prostate relationship, challenging the view of the EUS as merely a circular muscle. The findings demonstrate the importance of the EUS's extension into the prostate for urethral stabilization and continence. Recognizing this anatomy is crucial for maintaining urinary continence in prostate surgeries and enhancing postoperative outcomes.

Preventing Fungal Spoilage from Raw Materials to Final Product: Innovative Preservation Techniques for Fruit Fillings.

Spoilage fungi are a significant cause of financial loss in the food and beverage industry each year. These fungi thrive in challenging environments characterized by low acidity, low water activity and high sugar content, all of which are common in fruit fillings used in pastry products. Fruit fillings are therefore highly susceptible to fungal spoilage. Fungal growth can cause sensory defects in foods, such as changes in appearance, odor, flavor or texture, and can pose health risks due to the production of mycotoxins by certain mold species. To reduce food loss and waste and extend product shelf-life, it is critical that we prevent fungal spoilage. Synthetic chemicals such as sorbic acid and potassium sorbate are commonly used as preservatives to prevent fungal spoilage. However, with consumer demand for 'natural' and 'chemical-free' foods, research into clean-label preservative alternatives to replace chemical preservatives has increased. The objectives of this review are (i) to provide an overview of the sources of fungal contamination in fruit filling production systems, from pre-harvest of raw materials to storage of the final product, and to identify key control factors; and (ii) to discuss preservation techniques (both conventional and novel) that can prevent fungal growth and extend the shelf-life of fruit fillings.

Implementation and Mathematical Modelling of Data Sharing and Privacy Preserving Technique in a Blockchain Network by Using Machine Learning Algorithms

Blockchain technology has emerged as a reliable and secure decentralized network with multifaceted applications in banking, finance, insurance, healthcare, and business domains. Recent trends within blockchain communities indicate a growing interest in deploying machine learning models to extract valuable insights from extensive, geographically dispersed datasets owned by individual participants. To enable learning models without centralized data repositories, extensive research has focused on developing machine learning algorithms tailored for blockchain networks. However, despite numerous proposals, privacy and security concerns remain inadequately addressed, revealing vulnerabilities in architecture and operational efficiency limitations. The proposed ensemble machine learning model presents a pioneering solution, aiming to systematically resolve privacy, security, and performance issues within blockchain systems. The novelty of this approach lies in its targeted resolution of critical challenges at the intersection of blockchain technology and machine learning. While prior research has delved into integrating machine learning into blockchain networks, this model stands out by introducing a privacy-centric methodology that systematically addresses the core issues of privacy, security, and performance. Moreover, the proposed model promises enhanced resilience against adversarial attacks compared to other aggregation rules within a differentially private scenario. The innovative ensemble machine learning model for blockchain exhibits a significant 20% improvement in data privacy, a 15% boost in security, and a notable 25% enhancement in operational performance.

Middle Vault Dorsal Augmentation in Rhinoplasty as "Push-up" Preservation Technique or as "Modified Dorsal Split Extended Push-up" Procedure.

Primary or secondary dorsal augmentation rhinoplasty addresses aesthetic and functional issues related to a deficient, under-projected, or depressed nasal dorsum, frequently in middle vault. Traditional treatments often involve costal cartilage grafts, which can result in palpable irregularities and additional morbidity. Implementing dorsal preservation techniques in selected patients may improve predictability and outcomes of dorsal augmentation. We conducted a case series involving 12 patients with saddle nose deformities and significant nasal function impairment. All patients underwent either "push-up" preservation technique or "modified dorsal split extended push-up" technique. The techniques were evaluated for feasibility, safety, and efficacy. Functional outcomes were assessed using the nasal obstruction symptom evaluation (NOSE) scale preoperatively and three months postoperatively. Eight patients underwent "push-up" technique, and four patients required "dorsal split extended push-up" technique due to extensive scarring and mucosal adhesions from previous surgeries. All patients demonstrated significant postoperative improvement in nasal function as indicated by a reduction in NOSE scores (p < 0.05). Aesthetic outcomes showed a naturally augmented and smooth dorsum with no palpable irregularities. Patient satisfaction was rated very high (9 or 10 out of 10) in 10 patients and high (7 or 8 out of 10) in 2 patients. Follow-up was 6months. "Push-up" preservation technique and its extended variant provide a new surgical alternative for primary and secondary rhinoplasty with costal cartilage. These techniques offer improved and highly predictable aesthetic outcomes of the nasal dorsum with decreased morbidity, demonstrating significant potential for clinical practice and future research. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Herbarium specimens as tools for exploring the evolution of fatty acid-derived natural products in plants.

Plants synthesize natural products via lineage-specific offshoots of their core metabolic pathways, including fatty acid synthesis. Recent studies have shed light on new fatty acid-derived natural products and their biosynthetic pathways in disparate plant species. Inspired by this progress, we set out to develop tools for exploring the evolution of fatty-acid derived products. We sampled multiple species from all major clades of euphyllophytes, including ferns, gymnosperms, and angiosperms (monocots and eudicots), and we show that the compositional profiles (though not necessarily the total amounts) of fatty-acid derived surface waxes from preserved plant specimens are consistent with those obtained from freshly collected tissue in a semi-quantitative and sometimes quantitative manner. We then sampled herbarium specimens representing 57 monocot species to assess the phylogenetic distribution and evolution, of two fatty acid-derived natural products found in that clade: beta-diketones and alkylresorcinols. These chemical data, combined with analyses of 26 monocot genomes, revealed a co-occurrence (though not necessarily a causal relationship) between whole genome duplication and the evolution of diketone synthases from an ancestral alkylresorcinol synthase-like polyketide synthase. Limitations of using herbarium specimen wax profiles as proxies for those of fresh tissue seem likely to include effects from loss of epicuticular wax crystals, effects from preservation techniques, and variation in wax chemical profiles due to genotype or environment. Nevertheless, this work reinforces the widespread utility of herbarium specimens for studying leaf surface waxes (and possibly other chemical classes) and reveals some of the evolutionary history of fatty acid-derived natural products within monocots.

Lipidomics unveils changes in oxidised lipids in human breast milk by high‐pressure processing

SummaryHuman breast milk is a complex fluid rich in bioactive molecules crucial for infant growth and development. Global health organisations recommend exclusive breastfeeding to optimise infant health. In cases where maternal milk is unavailable, donor breast milk from human milk banks (HMBs) serves as an essential alternative. Currently, human breast milk preservation relies on Holder pasteurisation (HoP), which, while effective at pathogen inactivation, can compromise breast milk's nutritional and biological properties. High‐pressure processing (HPP) is an alternative pasteurisation method that utilises high pressure to kill pathogens without degrading nutrition. This study examined HPP's impact on donor breast milk lipids, sugars and antioxidants. Metabolomics reveals diverse bioactive compounds, including lipids, polyphenols and polyketides, elucidating breast milk's complexity. Some lipid subclasses respond to HPP; ceramides decreased under both treatments, while fatty aldehydes and esters increased under specific HPP conditions. Additionally, we quantified oxidised lipids, notably dietary oxysterols (DOxS), known for pro‐oxidant and pro‐inflammatory properties. These findings enhance understanding of HPP targeted approaches and breast milk's complexity, contributing to innovative milk preservation techniques that could advance specialised infant nutrition and assist donor milk banking systems.

Effects of ultrasound with slightly acid electrolytic water on storage of sweet potato: Physiological, nutritional, sensory and microstructural characteristics

Effects of ultrasound (US, 300, 400 and 500 W) combined with slightly acid electrolytic water (SAEW) at 25, 40 and 55 °C on physiological, nutritional, sensory and microstructural characteristics of sweet potato tuberous roots (TRs, Pushu 32) during 10, 30 and 60 d of storage were investigated, to explore a potential postharvest preservation technique for TRs. Sweet potato TRs by US500+SAEW50 exhibited the strongest PAL activity (10.63 U g−1) at 30 d of storage and SOD activity (83,707.42 U g−1) at 60 d of storage, suggesting the activation of strong defense system. Sweet potato TRs by US500+SAEW50 also showed integral cell walls, and presented the highest sensory acceptability (73.45). In addition, rotting rate of the TRs treated by US500+SAEW50 was reduced by 55% after 6 months of storage as compared to the untreated TRs in industrial application. Therefore, US500+SAEW50 could be a promising postharvest preservation technique for sweet potato TRs.

Extension of chicken fillets shelf life using genetically improved probiotic strain and chitosan

Fresh poultry meat products are perishable and need to be protected from spoilage. New or modified preservation techniques are needed to overcome the limitations of the traditional techniques. Therefore, six different coating solutions were evaluated to extend the shelf life of chicken fillets under refrigeration condition. The novel chitosan-based mix (T6) containing a bacteriocins-hyperproducer fusant, EDTA and sodium acetate was the treatment of choice since it improved film properties such as the reduction of water vapor permeability and the enhancement of the antimicrobial effect. Coating chicken fillets with the T6 mix resulted in improving both of the quality and shelf life of fillets. T6 recorded the lowest TBA value (1.25) compared to 4.77 mg malondialdehyde Kg−1 of the control at the end of the cold storage. Also, it extended the microbial shelf life up to 35 days by extending both of the lag phase and generation time of spoilage microorganisms by 6 days and 44.31 h, respectively. At the same time, T6 samples exhibited the highest sensory scores. It is strongly believed that such novel preservation system could be easily applied to extend the shelf life of many of the fresh foods since it was successfully applied to extend the shelf life of fresh chicken fillets by more than 31 days compared to control with high quality.

In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity

The ovarian cortical reserve of follicles is vital for fertility. Some medical treatments are toxic to follicles, leading to premature ovarian insufficiency. Ovarian tissue cryopreservation is an established method to preserve fertility in adults and even applied in prepuberty despite unproven efficacy. Here, we analyze transcriptomes of 120 cortical follicles from children and adults for detailed comparison. We discover heterogeneity with two main types of follicles in both age groups: one with expected oocyte-granulosa profiles and another with predicted role in signaling. Transcriptional changes during growth to the secondary stage are similar overall in children and adults, but variations related to extracellular matrix, theca cells, and miRNA profiles are found. Notably, cyclophosphamide dose correlates with interferon signaling in child follicles. Additionally, morphology alone is insufficient for follicle categorization suggesting a need for additional markers. Marker genes for early follicle activation are determined. These findings will help refine follicular classification and fertility preservation techniques across critical ages.

From Waste to Wealth: Exploring the Bioactive Potential of Wine By-Products-A Review.

The present paper explores the biological potential of bioactive compounds present in wine industry wastes, highlighting their valorization to promote sustainability and circular economy. Wine by-products, such as grape pomace and vine shoots, contain a high concentration of polyphenols, flavonoids, anthocyanins and other phytochemicals with antioxidant, anti-inflammatory and anticarcinogenic properties. Both conventional extraction methods, such as solid-liquid extraction, and emerging technologies, including enzyme-assisted extraction, ultrasound-assisted extraction, supercritical fluid extraction, microwave-assisted extraction, pressurized liquid extraction, high-hydrostatic-pressure extraction, and deep natural solvent-assisted extraction (NaDES), are discussed. In addition, the preservation of polyphenolic extracts by microencapsulation, a key technique to improve the stability and bioavailability of bioactive compounds, is addressed. The combination of advanced extraction methods and innovative preservation techniques offers a promising perspective for the valorization of bioactive compounds from wine residues, driving sustainability and innovation in the industry.

Mechanisms of slow-release antibacterial properties in chitosan‑titanium dioxide stabilized perilla essential oil Pickering emulsions: Focusing on oil-water interfacial behaviors

Perilla essential oil (PLEO) offers benefits for food preservation and healthcare, yet its instability restricts its applications. In this study, chitosan (CS) and TiO2 used to prepare composite particles. TiO2, after being modified with sodium laurate (SL), was successfully introduced at 0.1 %–3 % into the CS matrix. The resulting CS-SL-TiO2 composite particles can be formed by intertwining and rearranging through intramolecular and intermolecular interactions, and form an O/W interface with stability and viscoelasticity. The Pickering emulsions stabilized by these particles exhibit non-Newtonian pseudoplastic behavior, shear-thinning properties, and slow-release characteristics, along with antibacterial activity. Emulsions with 0.5 % and 1 % CS-SL-TiO2 composites demonstrated superior antibacterial effects against Escherichia coli and Staphylococcus aureus. The study revealed that all emulsions undergo Fickian diffusion and a sustained release of PLEO, with the Ritger-Peppas model best describing this release mechanism. The slow-release behaviors positively correlates with interfacial pressure, composite particle size, composite particle potential, composite contact angle, emulsion particle size and emulsion potential, but negatively correlates with diffusion rate, penetration rate, release kinetics and release rate. The findings lay groundwork for developing slow-release antimicrobial emulsions within polysaccharide matrices, showcasing promise for antimicrobial packaging solutions and enhanced food preservation techniques.

Employing Cryptographic Techniques for Data Security and Privacy Preservation

Employing Cryptographic Techniques for Data Security and Privacy Preservation

Implementing Privacy Preserving Techniques in Distributed Big Data Framework on Cloud Computing

Cloud-based deployment architectures have emerged as the preferred model for Big Data operations due to their scalability, flexibility, and cost-effectiveness. However, this shift raises new security concerns as data is no longer directly under the user's control. Securing Big Data in cloud environments is crucial for widespread adoption, but developing a comprehensive security plan is challenging without a thorough analysis of potential vulnerabilities.To address this, a novel security-by-design framework for Big Data deployment on cloud computing, termed Big Cloud, is proposed in this article. The framework leverages systematic security analysis methodologies and automated assessment tools to map domain-specific security knowledge to design best practices. Validation of the framework was conducted through an Apache Hadoop stack use case, demonstrating its effectiveness in enhancing security awareness and reducing design time.The study also evaluates the framework's strengths and limitations, identifying key challenges in the Big Cloud domain.