Preparation Of Mounts Research Articles

Preparation of Temporary or Permanent Mounts of Microfungi with Undisturbed Conidia

Preparation of Temporary or Permanent Mounts of Microfungi with Undisturbed Conidia

Preparation of capillary mounts in a dry box

Preparation of capillary mounts in a dry box

Preparation of full-thickness flat mounts of rabbit cornea. A new approach.

Preparation of full-thickness flat mounts of rabbit cornea. A new approach.

Techniques for the rapid preparation of permanent slides of microscopic algae

Because most techniques for the preparation of permanent mounts of algae have hitherto involved long alcoholic dehydration series, which frequently result in cell collapse if cut short, a method has been developed which is anhydrous from the start and avoids these series. It can be used to produce rapidly, permanent, generally-stained mounts of most microscopic algae in which the degree of shrinkage of the cell or its contents is reduced to a minimum. A method for the cleaning and mounting of diatoms is also given.

An Improved Method for the Preparation of Whole Organ Mounts for Light Microscopy: Localization of Calcium Salts and Correlative Radiographic Technics using Whole Breasts

An Improved Method for the Preparation of Whole Organ Mounts for Light Microscopy: Localization of Calcium Salts and Correlative Radiographic Technics using Whole Breasts

Techniques for Preparing Slide Mounts of Female Genitalia of the Khapra Beetle,Trogoderma granarium, and Related Species2

Techniques for the preparation of slide mounts of female genitalia were developed during an investigation to determine taxonomic differences in the genitalia of the khapra beetle, Trogoderma granarium Everts, and related species.

A technique for the measurement of soft tissue pressures acting upon teeth

A recording system has been developed suitable for making simultaneous pressure records from two intra-oral sensors. The system showed a linear sensitivity of 1 mm base line deflection for 2.7 g/cm 2 change of pressure over a range of 0–220 g/cm 2. There was no temperature sensitivity over a range of 25 °–38 °C. Response time for a half scale deflection was approximately 16 msec. Sensors were made as small as 3.5 mm dia. by 1.5 mm thick and could be attached directly to teeth by warm gutta percha and Eastman 910 cement requiring no prior preparation of special mounts.

The Preparation of Skeletal Mounts

The Preparation of Skeletal Mounts

Imprégnations rapides à la Nigrosine applicables à la micro-ecologie des surfaces

The ecology of primary biological coatings (fouling films) is an important aspect of benthic ecology in general. Life in a fouling film is, to a large extent, governed by physico-chemical laws of contact surfaces and surface tension. These laws apply also to the microecology of the “mesopsammon” and surface microplankton. The conditions common to these ecological situations enforce common characteristics, both morphological and physiological, upon the organisms which inhabit them. Many of these organisms are therefore subject to certain general techniques. Nigrosin is employed as an impregnating agent, and the speed of activity of the micelles of nigrosin is under the control of 1) the colloidal nature of the medium, and 2) alteration of cytoplasmic structures by the action of formalin added to the solution as a fixative. The stain blackens a variety of organites having a filamentous ultrastructure: cilia, flagella, trichocysts, rhabdites, cnidocysts, spiny processes of thecae and shells, capsules, cuticles and cuticular setae. The excess nigrosin surrounding the specimen may be removed completely by simple washing. Two particular situations are discussed: that of microbiocoenoses which develop at the point of contact of water and air (for example, bacterial films) and that of glass slides immersed in water with a view to studying the organisms which colonize surfaces in a particular habitat. The method has been applied also for studies of plankton in general, to demonstrate the liberation of flagellate bodies by certain algae, and for preparation of mounts of various organisms such as rotifers, gastrotrichs and nematodes. The method is very useful for quickly obtaining a comprehensive picture of the protozoan fauna in ecological studies.

Preparation of Permanent Mounts of Stained and Unstained Urinary Sediments

Preparation of Permanent Mounts of Stained and Unstained Urinary Sediments

A Method for Studying the External Anatomy of Copepods

We have found lactic acid to be the best clearing agent for the preparation of temporary mounts of whole or dissected copepods. Fresh, alcoholic, or formalin fixed specimens become cleared within a few minutes to some hours, depending upon their size and the duration of preservation. When first placed in the un diluted acid, the copepods may become somewhat contracted, but soon regain, and thereafter retain, their normal size and shape. In order to avoid rupture, obese forms or specimens with a thin cuticle are best transferred through mixtures of the medium in which they have been kept and lactic acid. Since the latter is dense, layering the fluids in a small dish is usually satisfactory: the original medium will evaporate slowly, leaving the speci mens in the acid. Fluids with appreciable concentrations of dissolved salts, however, should be avoided, preferably by transferring the specimens first either to alcohol or to fresh-water.

The "periosteal spread" technic for study of Mast cells.

SummaryA technic is described for the study of mast cells on such “natural tissue spreads” as the external periosteum of the calvarium and the dura mater of small laboratory rodents. These membranes can be fixed as flat sheets, while still attached to their normal osseous base, without the use of the customary traumatic procedures incident to the preparation of artificial tissue mounts.

An improved Technique for Permanent Mounts of small Insects and Nematodes

An improved technique is described for the preparation of permanent mounts of small Arthropoda and Nematoda, using a phenol-balsam mounting medium; a method is also described for clearing live, or freshly killed, specimens in strong chloral hydrate solution. The essential feature of the mounting method is the transfer of the specimen to a slide, in a drop of phenol, to which dry, powdered balsam is added and allowed to dissolve in situ. The mount is then dried and later covered in the usual fashion.

STUDIES OF THE MOTILE CELLS OF CHYTRIDS. I. ELECTRON MICROSCOPE OBSERVATIONS OF THE FLAGELLUM, BLEPHAROPLAST AND RHIZOPLAST

RECENT OBSERVATIONS with the electron microscope have revealed considerable detailed information about flagellar structure. Three fungi have been studied: Saprolegnia ferax by Manton et al. (1951, 1952); Allomyces arbuscula and Olpidium brassicae by Greenwood and Clarke (in Manton et al., 1952). Of these, the holocarpic species, Olpidium brassicae, is in the Chytridiales. This paper presents a study of the structure of the flagellum, blepharoplast and rhizoplast in two eucarpic chytrids, Chytridium sp. and Rhizophidium sphaerotheca, and observations in certain other fungi and bacteria. METHODs.-All the species observed were in pure culture except Nowakowskiella sp. and Saprolegnia sp., which were unifungal. In the preparation of mounts for the electron microscope, zoospores either with or without fixation with the fumes of 2 per cent osmium tetroxide, were placed on collodion coated wire grids and dried either very rapidly or slowly. In order to photograph the bacterial flagella of Actinoplanes organized in a single tail, as in fig. 25, it was necessary to omit fixation and to rapidly dry a small drop of swimming spores by careful blotting while holding the wire grid in the breeze of a fan. The flagellar sheath in Chytridiuim sp. was found after similar treatment or after exposure to osmic acid fumes for 15-30 sec. only, followed by rapid drying. It was usually necessary to wash the dried preparations several times with distilled water to remove as much of the agar culture medium as possible. The preparations were taken to the Department of Engineering Research at North Carolina State College where they were examined and photographed with an RCA Type EMU-2c electron microscope. The magnification dial of the microscope was calibrated with polystyrene latex particles which have a diameter of 2,500 + 50 A. So much variation in magnification is encountered on different occasions from the dial readings that the magnifications given for the most part are not very reliable. Size measurements were made against the latex particles on unshadowed material. The figures included here are of preparations shadowed with chromium. CHYTRIDIUM SP.-Figure 1 shows an entire zoo-

Preparation of Copepod Mounts for Taxonomic Work and for Permanent Collections

DURING an investigation of the microfauna of the shore at Whitstable, Kent, it was necessary to examine and to identify the many Harpacticoid copepods which occurred there. At the same time it was desirable to build up a type collection of these animals.

A method for the preparation of permanent mounts of India ink-injected specimens

A method for the preparation of permanent mounts of India ink-injected specimens

Preparation of Insect Mounts

Other| March 01 1954 Preparation of Insect Mounts Mary Benita Pieper Mary Benita Pieper Search for other works by this author on: This Site PubMed Google Scholar The American Biology Teacher (1954) 16 (3): 69. https://doi.org/10.2307/4438589 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Mary Benita Pieper; Preparation of Insect Mounts. The American Biology Teacher 1 March 1954; 16 (3): 69. doi: https://doi.org/10.2307/4438589 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentThe American Biology Teacher Search This content is only available via PDF. Article PDF first page preview Close Modal You do not currently have access to this content.

Rapid permanent staining and mounting of skin scrapings and hair.

THERE has been in use in this laboratory for several months a procedure for staining and mounting skin scrapings and hairs which is exceedingly simple and rapid, yields preparations and produces slides of a quality equal to or better than those obtained by more complicated methods.1The use of polyvinyl alcohol-lactophenol-cotton blue2permits more rapid preparation of permanent mounts than does the new method, but fungus structures stained with the PVA medium lose their color quickly and cotton blue so applied penetrates clinical materials poorly. Tinea versicolor scales, prepared by the method about to be detailed, have remained in excellent condition for seven months (fig. 1), as have skin scrapings containing Trichophyton hyphae (fig. 2). The general procedure will be described first, followed by suggested modifications for different categories of clinical specimens. A. MATERIALS REQUIRED The following materials are required for the procedure: 1. Solution of methylene blue hydrochloride, prepared

Preparation of Slide Mounts of Lepidopterous Genitalia

One of the most essential procedures in the identification of many Lepidoptera is an examination of the genitalia, and for a most critical examination of these organs slide mounts must be prepared. In many cases, the preparation of slides is one of the most neglected arts in lepidopterology. This is to be deplored, for a well-prepared slide is not only handsome but also much more valuable taxonomically than a messily prepared one. For large and medium-sized specimens, I have found the following technique very gratifying in producing slides of uniformly high quality. Certain phases of the technique are not particularly well adapted for working with smaller moths.

SOME EFFECTS OF MALEIC HYDRAZIDE ON SUGAR BEET PLANTS

S OF THE PAPERS PRESENTED BEFORE THE BOTANICAL TEACHING, GENERAL, MICROBIOLOGICAL, PALEOBOTANICAL, PHYSIOLOGICAL, AND SYSTEMATIC SECTIONS OF THE BOTANICAL SOCIETY OF AMERICA, COLUMBUS, OHIO, SEPTEMBER 11 TO 13, 1950 BOTANICAL TEACHING SECTION (Abstracts of this section are arranged alphabetically according to author) DAVIS, DONALD E., Alabama Polytechnic Institute, Auburn, Ala. Objectives of a general botany course in an agricultural college.-The obj ective of the first term may be divided into two categories: one is strictly utilitarian and the other is cultural. These categories are not wholly independent nor are they incompatible. These are six main objectives in the utilitarian category: (1) microscope technique and preparation of fresh mounts; (2) basic knowledge of food synthesis and utilization; (3) some understanding of the mechanism and significance of movement of materials into, within, and out of plants; (4) general understanding of plant structure and growth; (5) names and characteristics of some common trees; and (6) a minimum of terminology. The six main objectives in the cultural category are: (1) understanding of the scientific method as applied to both botany and other fields, with special emphasis on evaluation and interpretation of data, formulation of hypotheses, and what constitutes proof; (2) ability to understand cause and effect relationships as distinguished from the idea of purposeful behavior; (3) proficiency in solving new problems involving plant phenomena; (4) knowledge of both the differences and similarities between green plants and animals; (5) appreciation of the dependence of man and other animals on green plants; and (6) familiarity with names and characteristics of some common trees. Since transfer of learning can not be assumed, a constant effort must be made to show the interrelation and application of the material in this course to other courses and to everyday life. Daily lesson plans, demonstrations, and tests should all be evaluated on the basis of how much they contribute to these objectives. JOHNSON, MINNIE MAY, Stephens College, Columbia, Mo. Aerial field trips: preparation, follow-up and value.-Aerial field trips constitute a part of the regular program in the botany classes at Stephens College. Two trips are made by each student: the first in the fall at the peak of autumn coloration and the second one in the spring as the leaves begin to develop. In preparation for these trips the students are shown kodachrome slides covering the area over which they will fly. They are encouraged to read or interpret each of the landscapes. Just before the flight each student is given a mimeographed copy of a resume of the high points of the trip. As a follow-up, the instructor discusses the trips with small groups of students. In this evaluation use is made of a Kodak table slide viewer and a slide view box which makes it possible for the students to observe kodachrome slides of the four seasons at one time. The students are agreed that these aerial field trips are valuable experiences. By this means they get an over-all picture of the seasonal aspects of vegetation, plant habitats, plant distribution, the relationship of the topography of the soil to agriculture, soil erosion, erosion control, and the effects of strip mining. SMALL, JOHN A., New Jersey College for Women,, New Brunswick, N. J. A state park as an adjunct in teaching general botany.-Taking a This content downloaded from 40.77.167.83 on Wed, 08 Jun 2016 05:32:07 UTC All use subject to http://about.jstor.org/terms