Prebiotics In Diet Research Articles

Nanotechnology impacting probiotics and prebiotics: a paradigm shift in nutraceuticals technology

This is proven for a long that the incorporation of probiotics and prebiotics in diet exhibits beneficial effects on intestinal and intrinsic health. Nevertheless, this may encounter loss of vitality all along the absorption in the gastrointestinal tract, leading to meager intestinal delivery of probiotic active ingredients. In recent times, nanotechnology has been passionately used to escalate the bioavailability of active ingredients. Versatile forms of nanoparticles (NPs) are devised to be used with probiotics/prebiotics/synbiotics or their different combinations. The NPs currently in trend are constituted of distinctive organic compounds like carbohydrates, proteins, fats, or inorganics such as oxides of silver and titanium or magnesium etc. This review critically explicates the emerging relationship of nanotechnology with probiotics and prebiotics for different applications in neutraceuticals. Here in this review, formulations of nanoprobiotics and nanoprebiotics are discussed in detail, which behave as an effective drug delivery system. In addition, these formulations exhibit anti-cancerous, anti-microbial, anti-oxidant and photo-protective properties. Limited availability of scientific research on nanotechnology concerning probiotics and prebiotics implies dynamic research studies on the bioavailability of loaded active ingredients and the effective drug delivery system by including the safety issues of food and the environment.

The prospects of probiotics and prebiotics in the diet of military personnel in extreme conditions

The prospects of using probiotics and prebiotics in the diets of military personnel in extreme conditions have been studied. Domestic and foreign publications were used as the research material using the database of the United States (US) National Library of Medicine, Medline, and PubMed search engine. The nutrition of military personnel in extreme conditions should aim to improve diets by including functional nutrition products in their composition, such as probiotics and prebiotics. Herein, presented the modern concept of targeted probiotics, as well as the advantages of metabolic probiotics (metabiotics). The study results of the combined prebiotic in military personnel under environmental and professional stress, as well as in crew members in a closed hermetic object, are presented. The positive effect of probiotics and prebiotics in persons of dangerous professions against the background of excessive physical exertion or having a reduced nutritional status was revealed. The study of the metabiotic Bactistatin confirmed its effectiveness as a means of nutritional and metabolic rehabilitation of military personnel with inadequate body weight. The inclusion in the diets of products that normalize the microbiom is recognized as one of the directions for improving the nutrition of the North Atlantic Treaty Organization military personnel. Probiotics and prebiotics in the US Army diets contributed to intestinal infection prevention and immune system strengthening, thereby increasing endurance, physical, and cognitive performance during combat stress, and thus improving adaptation in extreme conditions. Domestic and foreign experience in the use of probiotics and prebiotics in diets has shown the prospects of their use as a means to improve the health and the military-professional of military personnel in extreme conditions of life.

Inclusions of probiotics and prebiotics in diet for coral trout, Plectropomus leopardus nursery

Coral trout is one of marine fishery commodities with high economic value. This grouper fish has limited ability in the utilization of artificial feed. The aim of this study was to obtain information on the effectiveness of probiotics and prebiotics inclusions in diet to increase growth of coral trout. Fish was reared in fiber tanks 300-L with a density of 30 fish/tank. Fish weight and length were 3.7±0.1 g and 6.3±0.1 cm, respectively. Each tank was equipped with a flowing water system and aeration as a source of oxygen supply. The feed was formulated with 48% protein and 9% lipid. Treatments were inclusion of probiotics in diet (diet A), inclusion of mannan oligosaccharide/MOS (diet B), inclusion of probiotic + mannan oligosaccharide/MOS (diet C), and diet D as control. The study was conducted in a complete randomized design (CRD) with 4 treatments and 4 replications. Fish fed trial diets with a frequency of 3 times a day at satiation. The parameters observed were growth, feed efficiency, feed digestibility, bacteria density and immunity. The data obtained were analysed with anova followed by LSD. The results showed that diet with the probiotic content promoted better growth and feed efficiency of coral trout compared to the other treatments. The highest growth of coral trout fingerlings was obtained by fish fed diet A (added with probiotics) with a weight gain of 794.86%. While fish fed diet containing probiotics and prebiotic (diet C) showed a lower growth response (weight gain 641.26%) than those fed diet with probiotics and prebiotics (MOS) separately. Survival during the study for all treatments ranged from 90.00% to 93.33%.

Preliminary evaluation of purified lignin and hemicellulose as prebiotics candidates for Atlantic Salmon, Salmo salar L.

ABSTRACTA study was conducted to investigate the potential of purified lignin and hemicellulose as prebiotics in diets for Atlantic salmon, Salmo salar L., postsmolt in seawater (30 ppt) at 14.9 ± 1.2°C. Triplicate groups of fish (initial individual body mass of 101.6 ± 1.2 g) were fed either the fishmeal-dense (32% of fishmeal) control diet (A) or one of the nine diets (15% fishmeal) supplemented with lignin:hemicellulose ratios (% diet:% diet) of 0.05:0 (B), 0.25:0 (C), 0.50:0 (D), 1.00:0 (E), 0:0.05 (F), 0:0.25 (G), 0:0.50 (H), 0.05:0.25 (I), and 0.25:0.50 (J) for 12 weeks in a complete randomized experimental design. At the end of the experiment, the thermal-unit growth coefficient (TGC) was significantly lower in salmon fed diets with 0.50% and 1.00% purified lignin than those fed the control diet, while there was no significant difference between the other treatments and the control diet (P < 0.05). The feed efficiency (FE) was significantly lower in salmon fed diets with 0.50% and 1.00% purified lignin and 0.05% hemicellulose than those fed the control diet, while there was no significant difference between the other treatments and the control diet (P < 0.05). The hepatosomatic index (HSI), intestinal villus length, and apparent digestibility coefficient (ADC) of nutrients in diets were similar (P > 0.05) among all the diets. This study demonstrated that inclusions of up to 0.25% of dietary purified lignin alone, and 0.25%–0.50% of dietary hemicellulose alone or in combination with dietary lignin can be considered as candidate prebiotics in Atlantic salmon nutrition.

Human Gut Microbiota in Health and Alzheimer's Disease.

Gut microbiota plays a crucial role in human health and disease. The alterations in the composition of gut microbiota may cause the onset of certain human pathologies. One of these is Alzheimer's disease (AD). High-fat diets, administration of antibiotics, lack of probiotics and/or prebiotics in diet increase the risk of AD. On the other hand, modulation of the composition of gut microbiota may decrease the risk of AD and be able to slow down the progression of AD.

Protective effects of prebiotics inulin and lactulose from cytotoxicity and genotoxicity in human colon adenocarcinoma cells

The accepted role of dietary prebiotics is to improve probiotic survival and growth in the lower intestine, yet few studies have investigated the possibility that prebiotics may offer benefits beyond their role with probiotics. This study investigates the protective effects of the prebiotics inulin and lactulose against cytotoxic and genotoxic effects in HT-29 human adenocarcinoma colon cells caused by human faecal water, bile acids and 4-nitroquinoline-1-oxide (4-NQO). Using the MTT assay, deoxycholic acid, lithocholic acid and faecal water 50% v/v reduced cell viability by 75, 74 and 50% respectively. In the presence of inulin and lactulose (0.5–2.0%) cell survival increased by 100% for deoxycholic acid, by 30% for lithocholic acid and by 40% for faecal water. Both faecal water (50% v/v) and 4-NQO (1–2g/ml) were found to be highly genotoxic using the SOS chromotest, however incubation with inulin and lactulose (2%) significantly reduced the bacterial assay SOS inducing potency (SOSIP) by 70% and 57% respectively for faecal water and by up to 29 and 74% for 4-NQO. Similar protection against genotoxicity was observed with 4-NQO using the Ames fluctuation test. These tests show that prebiotics, in the absence of probiotics, were able to protect mammalian cells (HT-29 cells) against cytotoxicity and reduce the genotoxicity of known mutagens in bacterial mutation assays. Reproduction of these results in vivo would suggest that the inclusion of prebiotics in diet may directly confer health benefits beyond those associated with probiotic survival and growth.

Prebiotic effects of fermentable carbohydrate polymers may be modulated by faecal bulking of non‐fermentable polysaccharides in the large bowel of rats

SummaryThis study investigated the impact of supplementary fermentable polysaccharides inulin (a prebiotic) and barley β‐glucan on the large bowel, when consumed against a background high or low intake of mixed non‐starch polysaccharide. Rats were fed for 28 days with diets containing high (15%) and low (5%) background dietary fibre (BDF; cellulose plus sugar beet fibre), with or without 5% supplementary fermentable fibre (SFF; inulin or barley β‐glucan). High‐BDF diets unsupplemented with inulin or barley β‐glucan caused greater improvement than supplemented low‐BDF diets in several parameters linked to gut health: they increased colonic Bifidobacterium spp., butyric acid concentration in the caecum, and colonic crypt depth more than inulin and barley β‐glucan. For these parameters, inulin and barley β‐glucan added little to the effects of the high‐BDF diet alone. Furthermore, by allowing for dilution due to the greater faecal bulk induced by the mixed fibre compared with inulin or barley β‐glucan, the total quantity of butyric acid in the colon of BDF‐fed rats was greatly amplified. Interpretation of the effects of prebiotics in diets containing dietary fibre requires concurrent analysis of the multiple effects of non‐fermentable bulk.