Ppm IBA Research Articles

Combined effects of auxin application and beneficial microorganisms on rooting and growth of Ficus benjamina L. air-layers

This study was conducted to define the best auxin application method with or without the inoculation with beneficial microorganism (Bacillus subtilis and arbuscular mycorrhizal fungi “AMF”) to improve rooting characteristics of F. benjamina air-layers. The treatments were arranged in a split-plot design. Seven combined treatments of auxin concentrations and application methods (control, 50 and 100 ppm IBA in rooting substrate, 1500 and 3000 ppm IBA by painting girdled zone, 1500 and 3000 ppm IBA in talc paste) were assigned to the main plots.

Effect of indole-3-butyric acid application type and concentration on rooting efficacy in three endemic rare-endangered Dianthus species with conservation priority

Efficient propagation of uniform starting material is a critical requirement for mass production of most ornamental plants. Conservation and protection of rare-threatened plants and sustainable exploitation of biodiversity can be achieved through ex situ conservation actions. Therefore, propagation experiments were carried out on three endemic Caryophyllaceae species with limited spread and conservation priority: Dianthus ingoldbyi, Dianthus juniperinus subsp. bauhinorum and Dianthus fruticosus subsp. occidentalis. Softwood top cuttings were used in the middle of winter for D. fruticosus (6-7 cm) and D. juniperinus (5-6 cm) and in early spring for D. ingoldbyi (4-5 cm). The base of cuttings was immersed for 1 min in solutions of four IBA concentrations (0, 1000, 2000 and 4000 ppm) and also dusted with 0.066% and 0.2% IBA (in powder form). The cuttings were placed in a peat: perlite (1:3) substrate on a heated greenhouse mist. The most appropriate treatment for D. ingoldbyi (7 weeks) was 2000 ppm IBA (15.07 roots3.06 cm long, 100% rooting). In D. juniperinus, 1000 ppm IBA gave the highest rooting (57.14%) with 39.75 roots 7.49 cm long (8 weeks). Rooting of D. fruticosus was more effective (45.33 roots 5.85 cm long, 28.57% rooting) with 1000 ppm IBA (8 weeks).

Asexual Propagation of Four Medicinal Greek Endemic Plants of Lamiaceae Family With Conservation Priority From The Collection of The Balkan Botanic Garden of Kroussia, N. Greece

Conservation of endemic rare-threatened plants and sustainable exploitation of biodiversity with emphasis on medicinal-aromatic plants and plants with horticultural/ornamental value can be achieved through ex situ conservation activities. For this purpose, propagation experiments with cuttings were performed on four local Ionian endemic species with conservation priority, Stachys ionica Halácsy, Teucrium halascyanum, Thymus holosericeus Celak and Thymus plasonii Adamovic (all Lamiaceae). For propagation, softwood tip cuttings (3-5 cm) were cut at early autumn from mother plants collected from the wild and maintained in open-air mother plantations. For experimentation, the base of cuttings was immersed for 1 min in solutions of four concentrations of IBA (0, 1000, 2000 and 4000 ppm). Cuttings were placed on a peat:perlite (1:3) substrate in the bench of greenhouse heated mist system. Most suitable treatment for T. halascyanum (3 ½ weeks) proved to be 1000 ppm IBA (32.13 roots 1.72 cm long, 100% rooting). Accordingly, 2000 ppm IBA gave 100% rooting for both S. ionica (28.5 roots 1.56 cm long,) (3 weeks) and Th. holosericeus (4.4 roots 1.76 cm long) (7 weeks). T. plasonii cuttings treated with 2000 ppm IBA gave 85.71% optimum rooting with 8.67 roots 1.78 cm long.

Vegetative propagation of Lisianthus genotypes through stem cuttings: a viable alternative to seed propagation

Lisianthus is a potential new flower crop that has been recently introduced in India. The propagation of lisianthus through seeds has emerged as a great challenge for its commercialization. In the present study we have explored the possibility of vegetative propagation of lisianthus crop through stem cuttings. The effect of different doses of growth regulators viz. Indole-3-butyric acid (IBA: 250&500 ppm) and naphthalene acetic acid (NAA: 250&500 ppm) either alone or in combinations on rooting of stem cuttings was assessed in seven lisianthus genotypes. Treatment of lisianthus cuttings with 250 ppm IBA for 5 minutes induced the maximum rooting. The number of roots per cutting, root length and fresh and dry weight of the root was found maximum when the cuttings were treated with 250 ppm IBA for 5 minutes. The genotypes Echo Double Pink Picotee, Echo Double White and Echo Double Blue exhibited more than seventy percent rooting indicating the scope for their vegetative propagation. Among the different lisianthus genotypes, the maximum rooting was observed in Echo Double Pink Picotee. The maximum number of roots per cutting (11.6) and the maximum root length (7.9 cm) was observed in Echo Double Blue. When the lisianthus crop raised through rooted cuttings and seedling methods were compared, it was observed that the crop raised through rooted cuttings performed better than the seedling raised crop. The vegetative propagation through stem cuttings can be a viable alternative for the propagation of lisianthus.

Effects of Auxin and Media Additives on the Clonal Propagation of Guava Cuttings (Psidium guajava L.) Var. Chinese Gola

Clonal propagation is a process of asexually reproducing plants by multiplication of one copy to produce several copies that are genetically identical. One of the best methods of clonal propagation is using the cuttings. There are many plants that can be propagated through cuttings and one of them is guava. Guava is popular in Pakistan. To ensure that a guava tree will produce fruits that belong to its original cultivar, this study was conducted. Softwood cuttings of guava were treated with IBA (1600 ppm and 2000 ppm), NAA (7000 ppm and 10,000 ppm) and IBA+NAA (1600+7000 ppm and 2000+10,000 ppm). There were five media used (Soil, Peatmoss+Soil, Sand+Soil, Baggasse+Soil and Soil+Peatmoss+Sand+Bagasse. The experiment was laid out using Completely Randomized Block Design (CRBD) with two-factor factorial arrangement. A total of 105 cuttings were used, each with 12 cm length and with 2-4 nodes. Media samples were collected and physio-chemically analyzed at the Soil and Water Testing Laboratory in Layyah, Pakistan to assess the properties and fertility status. Guava cuttings with 1600 ppm IBA showed the best results in terms of the number of roots, root length and days to sprout, followed by 7000 ppm NAA in Peat moss+soil and soil+peat mass+sand+bagasse. For the combination of growth regulators, the best results were observed in IBA 1600 ppm + NAA 7000 ppm. Poorest result in all parameters (days to sprout, number of roots, and root length) was recorded in control. The 1600 ppm IBA with 10 second dipping time for softwood cuttings of guava yielded best results in peatmoss+soil media for all the parameters tested. Hence, this study concluded that treatment with auxin (such as IBA) can yield best results and can help to solve the problem in propagating guava using cuttings.

Cutting propagation of European hazelnut cultivar ‘Tombul’

This research was conducted with the aim to improve the rooting efficiency in stem cuttings of the most cultivated Turkish hazelnut cultivar 'Tombul'. In the present study, different solution of 3-indolbutyric acid (IBA) (0, 1,000, 2,000, 3,000, 6,000, 8,000 and 10,000 ppm) were applied to hardwood cuttings by quick-dip method for five seconds. In addition, hardwood cuttings were dipped in 0, 1,000, 2,000 or 3,000 ppm IBA solutions adopting the same method and inserted into the perlite medium soaked with 0, 1, 2, 3 g L(-1) concentrations of polyvinyl alcohol (PVA). For softwood cuttings, quick-dip method with IBA solutions (0, 500, 1,000, 2,000 and 3,000 ppm) or soaking method with IBA solutions (0, 50, 100, 150, 200, 300, 400 and 500 ppm) during four hours have been applied. After eight weeks, average rooting rate, number of roots per cuttings, root length, rooting level (0.1-4.0), fresh and dry weights and shoot development were determined. The highest root rates (26.7-43.3%) and the root development of 'Tombul' hazelnut were obtained in softwood cuttings with 500, 1,000, 2,000 and 3,000 ppm concentrations of IBA by quick-dip method. Rooting has not been achieved in 'Tombul' hardwood cuttings using different concentrations of IBA with or without PVA.

Clonal propagation technique for mass multiplication of Noni (Morinda citrifolia) seedlings

A study was under taken to standardize the type of cutting, concentration of growth hormone and suitable rooting media to produce elite seedlings in Morinda citrifolia at Forest College and Research Institute, Mettupalayam. Investigation was carried out to standardize the treatments to induce root and shoot growth in different types of cuttings of Morinda citrifolia L. using two growth regulators (IAA, IBA) used in different concentrations (1000, 1500, 2000 and 2500 ppm) in two kinds growth medium (Sand and Coir) for different type of cuttings, 2500 ppm IBA treatment recorded its superiority in terms of maximum sprouting (85%), rooting (79%) and survival (70%) percentage, number of roots (15.83), root length (8.51 cm), number of shoots (4.00) and shoot length (9.43 cm) in leafy shoot cuttings, irrespective of rooting media used. From this current study it is concluded that, for macro clonal propagation of noni ( Morinda citrifolia L.), either leafy shoot cuttings or hard wood cuttings could be treated with 2500 ppm IBA or semi hard wood cuttings could be treated with 1500 ppm IAA and could be planted in coir pith medium for the production of elite planting materials.

Enhanced growth and development of Chrysanthemum morifolium in microponic system under light-emitting diodes

This paper investigated the growth and development of Chrysanthemum shoots in microponic systems in comparison with shoots in micropropagation system. A microponic culture system, combining micropropagation and hydroponics, could reduce the drawbacks of micropropagation system such as reduction of infection, saving of labor, material, space, etc. In this study, Chrysanthemum morifolium shoots with 3 cm in length were cultured in MC (microponic system with circular container - 12 cm diameter at top, 9 cm diameter at bottom and 8.5 cm in height), MR (microponic system with rectangular box - 8.5 cm in height, 35 x 28 cm at top and 30 x 25 cm at bottom) and micropropagation system (MO - rectangular plastic box with 800 ml of half-strength MS medium containing 30 g/l sucrose and 8 mg/l agar). The results indicated that shoots pretreated with 500 ppm IBA, cultured in MC (15 shoots per container) and ventilated with millipore membrane (MillisealTM, pore size 0.5 µm of diameter 2 cm) under 70% red LED combined with 30% blue LED gave thebest plant height, number of roots, fresh weight, and chlorophyll content (a, b and a+b) (5.18 cm, 12.50, 0.52 g, 28.19 µg/g, 13.56 µg/g and 41.75 µg/g, respectively). The survival rates of plants derived from MC and MR in the greenhouse were higher than those in MO (100%, 100% and 85%, respectively). This study indicated that MR was an effective and simple system for large-scale production of Chrysanthemum morifolium.

Effect of rooting hormone and media on root induction in poinsettia (Euphorbia pulcherrima Willd.)

An experiment was carried out to study the effect of different concentrations of IBA and rooting media on root induction of poinsettia (Euphorbia pulcherrima Willd). The experiment was laid out in factorial Completely Randomized Design (FCRD) with sixteen treatment combinations consisting four levels of IBA and four different types of media. Among the various levels of IBA, 4000 ppm IBA (C3) was most effective for obtaining number of sprouts per cutting (2.29), number of roots per cutting (16.42), length of shoot (6.85 cm), length of root (20.25 cm), number of leaves per cutting (14), fresh weight of root (1.56 g), fresh weight of shoot (11.98 g), dry weight of shoot (5.13 g), rooting percentage (76.40%) and survival percentage (74.19%) of rooted cuttings after 60 days of planting. Consequently, Red soil + Cocopeat (M4) media was found most promising for finding number of sprouts per cutting (3.30), number of roots per cutting (16.30), length of shoot (7.86 cm), length of root (23.02 cm), number of leaves per cutting (14.96), fresh weight of root (1.72 g), fresh weight of shoot (12.52 g), dry weight of shoot (5.49 g), rooting percentage (86.84%) and survival percentage (85.83%) for rooted cuttings after 60 days of planting. Cuttings planted in sand exhibited early sprouting as compared to other media. Interaction effect of different levels of IBA and rooting media was found non significant for all the characters.

Bakteri ve Oksin Uygulamalarının Kuşburnu Bitkisinin Çelikle Çoğaltılması Üzerine Etkileri

Rosa canina L. çelikleri sonbahar mevsiminde Ondokuz Mayıs Üniversitesi Kurupelit Kampüsü florasından temin edilmiştir. Yarı odunsu çelikler, sisleme ve alttan ısıtma sistemi olan köklendirme masasına, 1:1 oranında torf ve perlit karışımına dikilmiştir. Çalışmada 10 farklı rizobakteri (bitki gelişimini düzenleyen) izolatı tek başına ve 1000 ppm oksin (Indole-3-butyric acid-IBA) ile birlikte uygulanmıştır. Çelikler 6 saat bakteri uygulamasından sonra, dikim öncesi 10 saniye IBA çözeltisinde bekletilmiştir. Köklenme oranı, kök yumağı eni, kök boyu ve ana kök sayısı saptanmıştır. En yüksek köklenme oranı Bacillus megaterium, Bacillus megaterium ve Pseudomonas flourescens uygulamalarında %30 olarak saptanmıştır. Kontrolde %10 oranında köklenme görülürken, IBA bazı bakterilerin etkisini değiştirmemiş (Bacillus subtilis, Agrobacterium rubi, Paenibacillus polymyxa), bazılarında hafif düşüşe yol açmış (Bacillus megaterium), buna karşın tek başına hiç köklenme sağlamayan 3 bakteri izolatı (Bacillus megaterium, Bacillus subtilis, Pseudomonas putida) %10 oranında köklenme göstermiştir. Bakteri uygulamaları genel olarak, köklenme oranı dışında köklenme kalitesini (kök yumağı eni, kök boyu ve ana kök sayısı) artırmıştır.

Bakteri ve Oksin Uygulamalarının Kuşburnu Bitkisinin Çelikle Çoğaltılması Üzerine Etkileri

Rosa canina L. cuttings were obtained from the flora of Ondokuz Mayıs University campus of Kurupelit. Woody cuttings were placed into the medium of perlite and turf mixture with a ratio of 1:1 under mist propagation system with bottom heating system. Rosa canina cuttings were treated with 10 different plant growth promoting rhizobacteria (PGPR), alone or with the combination of 1000 ppm auxin (Indole-3-butyric acid-IBA), before planting into rooting medium. Cuttings were first kept in solution for 6 h and then treated with 1000 ppm IBA for 10 seconds. The rooting ratio, the diameter of rooting area, root length and the number of main roots were determined. According to the results, the highest rooting ratio was obtained from Bacillus megaterium (M-3), Bacillus megaterium (TV-60D) and Pseudomonas flourescens (TV-11D) bacteria treatments by 30% whereas rooting ratio was 10 % for control cuttings. IBA did not change the effects of some bacteria (Bacillus subtilis, Agrobacterium rubi, Paenibacillus polymyxa), slightly reduced the effects on some bacteria (Bacillus megaterium), whereas provided 10% rooting on some bacteria (Bacillus megaterium, Bacillus subtilis, Pseudomonas putida) which were not effective alone. In general, bacteria treatments improved the rooting quality (diameter of rooting area, root length and the number of primary roots) other than rooting ratio.

Effects of auxin doses onrooting of Juniperus L.

The present study was conducted to investigate the rooting ratios of cuttings taken from naturally grown Greek juniper trees of Gevas and Catak (Van) regions at different dates in 2013 and 2014 and under different hormone doses. The cuttings taken in October and December of 2013 and April of 2014 were immersed into water for 24 hours, then they were dipped into 3000 ppm, 5000 ppm and 7000 ppm NAA and IBA hormone solutions for 5 seconds. Cuttings were planted into growth mediums for rooting. For reliable rooting, the most proper hormone concentration was identified as 5000 ppm NAA and the most proper planting time was identified as April.

In Vitro Propagation of Temulawak (Curcuma xanthorrhiza Roxb.)

Problems encountered in the development of herbal medicine industry is that most of the raw materials (80%) came from the forest or natural habitats and the rest (20%) of the results of traditional cultivation. The research is focused to get the propagation techniques of temulawak by in vitro using various combinations of IBA and BAP concentration, each consisting of 0, 1, 2, 3, 4 ppm. The purpose of this research is to gain a plant propagation technique of temulawak through the use of IBA and BAP concentration so as to provide a quality seeds. The results showed that the highest shoots obtained at 1 ppm IBA and 3 ppm BAP treatment with a buds height of 15.9 cm. Treatment of 0 ppm IBA and 4 ppm BAP produces the highest buds number by 4 buds. While the highest number of leaves obtained in 2 ppm IBA and 3 ppm BAP treatment with total of 18 leafs. The highest length of leaf obtained in the treatment of 3 ppm IBA and 2 ppm BAP with a length of 14.6 cm. Variable highest number of roots was obtained at treatment of 4 ppm IBA and 1 ppm BAP with root number exceeds 30 pieces with very many hair roots and the highest root length was obtained at treatment of 2 ppm IBA and 0 ppm BAP with a length of 15.6 cm. Based on these results it can be concluded that the IBA and BAP treatment at various concentrations can affect the propagation and growth of temulawak explants.

Biomass Production of Root and Shoot of Talinum paniculatum Gaertn. by Liquid and Solid Ms Medium with Plant Growth Hormone IBA

Talinum paniculatum Gaertn. is one of traditional medicinal plant in Indonesia which has benefits such as for vitality and maintain blood circulation. The aim of this research is to obtain biomass production of root and shoot of T. paniculatum Gaertn. by liquid and solid MS medium with IBA. This research conducted to provide biomass as raw material for secondary metabolites test. Stems as explant were induced with four treatments (liquid MS, solid MS, liquid MS + 2 ppm IBA and solid MS + 2 ppm IBA) with five repetitions. Observation did for 28 days. The parameters are the percentage of explants which formed the root and shoot, morphology, fresh and dry biomass. Result shows that percentage of root and shoot have 100% in liquid and solid MS + 2 ppm IBA. Fresh and dry biomass of root and shoot in solid MS + 2 ppm IBA higher than the others. This research found callus in liquid and solid MS + 2 ppm IBA. Morphology of root in liquid MS has thin and friable, but thick in solid MS. Shoot in solid and liquid MS has thin, short and sturdy.

PENGARUH KONSENTRASI IBA TERHADAP KEMAMPUAN BERAKAR SETEK PUCUK Alstonia scholaris (L.) R. Br. SEBAGAI UPAYA PENYEDIAAN BIBIT UNTUK REVEGETASI

Alstonia scholaris (L.) R. Br. is an indigenous plant species in Indonesia which has been use for multiple purposes. This plant tolerant to poor nutrient and alkaline soil, therefore it is potential to explore as a revegetation plant. The study was conducted to identify effect of IBA concentrations on rooting ability of A. scholaris shoot cutting. Plant materials (shoot cutting) were collected from trubusan in Mandeh Area, Tarusan, West Sumatra. This study used Completely Randomized Design (CRD). The treatment was IBA concentrations (0 ppm, 50 ppm, 100 ppm, 150 ppm and 200 ppm). The result showed that IBA concentrations was significant different (P ? 0,05) for length, fresh weight and dry weight of root. The application of 150 ppm IBA was the best concentration to enhance the rooting ability of A. scholaris shoot cuttings.

Effect of node number and auxin concentration on propagation of ivy gourd (Coccinia cordifolia cogn) through stem cuttings

Field experiment was conducted during 2010-2011 to study the effect of number of nodes per cutting and concentrations of plant growth regulators (IBA and NAA) on regeneration of ivy gourd at Indian Institute of Vegetable Research, Varanasi. Cuttings with four nodes sprouted earliest (8.8 DAP) and enhanced the sprouting per cent, length and diameter of vine, number of branches per shoot, number of leaves per cutting, length and diameter of root and regeneration per cent than one, two and three nodes per cutting. Ivy gourd cuttings treated with 75 ppm IBA took minimum days to sprout and improved sprouting per cent, increased length and diameter of vine, number of branches per shoot, number of leaves per cutting, length and diameter of root and regeneration per cent than 75 ppm NAA and 25, 50, 100, 125 and 150 ppm IBA and NAA.

REGENERATION OF Pimpinella pruatjan THROUGH SOMATIC EMBRYOGENESIS

Pruatjan (Pimpinella pruatjan Molk.) is an Indonesian endangered plant which has various medicinal properties such as aphrodisiac, diuretic, and tonic. The plant is commonly harvested from its natural habitat, therefore it becomes endangered. Regeneration of pruatjan through organogenesis has been studied, but its shoot multiplication was very low (5 shoots per explant). The study aimed to investigate the best regeneration technique of pruatjan through somatic embryogenesis. This research was conducted at the tissue culture laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development in 2004-2005. Callus formation of pruatjan was induced from the petioles and leaves in Driver and Kuniyaki’s (DKW) based medium containing 2,4-D combined with picloram at the level of 0.5, 1.0, 1.5, and 1.5 ppm. Embryogenic calli were then transferred into embryo development medium in two ways. First, they were directly transferred into media containing IBA/NAA at the level of 0.5, 1.0, and 1.5 ppm. Second, they were indirectly transferred into media containing 2.0 ppm 2,4-D and 0.3% casein hydrolysate prior to the IBA/ NAA media. Parameters evaluated were fresh weight, dry weight, time initiation of embryogenic callus formation, and total number of embryos. The result showed that calli of pruatjan were successfully induced from the petioles and leaves. The best calli were induced from the leaves in the DKW medium containing 2.0 ppm 2,4-D and 0.5 ppm picloram. Embryo development of the calli was best if they were first grown in the media containing 2.0 ppm 2,4-D and 0.3% casein hydrolysate then transferred to the IBA/NAA media. The total number of somatic embryos was counted up to 103 on the medium containing 1.5 ppm IBA. This study indicated that pruatjan somatic embryogenesis regeneration required three different media, i.e. for callus induction, development and maturation, and for germination.

REGENERATION OF Pimpinella pruatjan THROUGH SOMATIC EMBRYOGENESIS

Pruatjan (Pimpinella pruatjan Molk.) is an Indonesian endangered plant which has various medicinal properties such as aphrodisiac, diuretic, and tonic. The plant is commonly harvested from its natural habitat, therefore it becomes endangered. Regeneration of pruatjan through organogenesis has been studied, but its shoot multiplication was very low (5 shoots per explant). The study aimed to investigate the best regeneration technique of pruatjan through somatic embryogenesis. This research was conducted at the tissue culture laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development in 2004-2005. Callus formation of pruatjan was induced from the petioles and leaves in Driver and Kuniyaki’s (DKW) based medium containing 2,4-D combined with picloram at the level of 0.5, 1.0, 1.5, and 1.5 ppm. Embryogenic calli were then transferred into embryo development medium in two ways. First, they were directly transferred into media containing IBA/NAA at the level of 0.5, 1.0, and 1.5 ppm. Second, they were indirectly transferred into media containing 2.0 ppm 2,4-D and 0.3% casein hydrolysate prior to the IBA/ NAA media. Parameters evaluated were fresh weight, dry weight, time initiation of embryogenic callus formation, and total number of embryos. The result showed that calli of pruatjan were successfully induced from the petioles and leaves. The best calli were induced from the leaves in the DKW medium containing 2.0 ppm 2,4-D and 0.5 ppm picloram. Embryo development of the calli was best if they were first grown in the media containing 2.0 ppm 2,4-D and 0.3% casein hydrolysate then transferred to the IBA/NAA media. The total number of somatic embryos was counted up to 103 on the medium containing 1.5 ppm IBA. This study indicated that pruatjan somatic embryogenesis regeneration required three different media, i.e. for callus induction, development and maturation, and for germination.

Respon Kunir Putih (Kaempferia rotunda) terhadap Pemberian IBA dan BAP pada Kultur In Vitro

<p><em>Kaempferia rotunda </em>that long ago been used to treat gastrointestinal disorders and also treat cancer, has<em> </em>promising opportunities to cultivate. There are several obstacles in cultivation today. Plant propagation by <em>in</em> <em>vitro </em>can be one solution.<em> </em>Basic media and plant growth promoting substance usage is an important factor which<em> </em>has to be concerned. Research aiming to produce high quality seed and in large numbers. Research has been carried out in Tissue Culture Laboratory, Agriculture Faculty, Sebelas Maret University, Surakarta, during July 2014 to August 2015. This research was used MS (Murashige and Skoog) media with combination treatment of each IBA and BAP concentration of 0, 1, 2, 3, and 4 ppm. The number of shoots that appear on the explant is the main observation variables. The results showed IBA and BAP are likely to affect the appearance of buds as much as 2 pieces in each explant at 0 ppm IBA treatment.</p>

Efisiensi Mikropropagasi Pisang Kepok Amorang melalui Modifikasi Formula Media dan Temperatur

<p>Micropropagation Efficiency of Banana cv Kepok<br />Amorang through Modifications of Culture Media and<br />Incubation Temperature. Yati Supriati. The budless<br />banana cv Kepok Amorang is potentially commercialized<br />due to its sweet taste and does not have flower bud, hence<br />reduced the potential of being infected by the blood disease<br />pathogen. Enhancement of banana industry needs continuous<br />supplies of large number banana seedlings. In vitro<br />culture enable the production of seedlings in a large scale,<br />uniform, quick. The research aims: (1) to formulate an<br />efficient medium for in vitro multiplication of cv Kepok<br />Amorang shoot, (2) to identify efficient growth environment<br />for in vitro culture of cv Kepok Amorang, and (3) to formulate<br />an efficient culture medium for roots inductions of cv<br />Kepok Amorang. The plant material used was in vitro culture<br />of Kepok cv Amorang, 2 cm in height without leaf and root.<br />The media formulation for shoot multiplication were full<br />strength, half strength, one fourth strength MS media,<br />supplemented with either 1, 3, or 5 ppm IBA. On optimization<br />step, the media tested were MS, Knop, Knop and<br />Heller, Hyponex N, Growmore N, and Rosasol N containing<br />of 1 ppm BA. The explants were incubated in culture room<br />with 8, 12, and 16 hours photoperiod with temperatures 30oC<br />(non air conditioned) and 25oC (air conditioned). The root<br />induction trial was done using MS, Knop, Knop and Heller,<br />Hyponex N, Growmore N, and Rosasol N media containing<br />of 1 ppm and 3 ppm IBA. The results showed that the best<br />medium formula for shoot multiplication was ¼ MS + 1 ppm<br />IBA. The best incubation condition was 16 hours photoperiods<br />at 30oC. The best media for root induction was<br />Hyponex 2 g/l + 1 ppm IBA. This culture method reduced<br />cost by Rp 261.7 per plantlet through efficiency of media<br />formulation and electricity use.</p>