Ppm Benzylaminopurine Research Articles

Pathogen‐tested material of grapevine varieties and rootstocks

Pathogen‐tested material of grapevine varieties and rootstocks

Establishment of western white pine shoots invitro using needle fascicles

The foliage of 4-year-old western white pine (Pinusmonticola Dougl.) containerized seedlings was treated with 450 ppm benzylaminopurine weekly, for 8 weeks, to induce the development of fascicle buds. Fascicle buds were cultured invitro on five different media. Gresshoff and Doy 1 medium and Gresshoff and Doy 1 with an increased concentration of potassium nitrate (1500 mg L−1 KNO3 was added) yielded greater bud elongation and secondary needle growth than other media tested. An invitro dip in 2000 ppm naphthaleneacetic acid promoted the formation of white glassy callus, potential root primordia, and root growth in 33% of the explants. Only 10% of the explants developed elongated roots 120 days after initial culture.

Influence of Temperature, Water Stress and BA on Vegetative and Reproductive Growth of Schlumbergera truncata1

Abstract The number of flower buds formed on plants of Schlumbergera truncata (Haw.) Moran (Thanksgiving cactus) was reduced by high water stress during flower initiation and was unaffected by night temperature (13° vs. 17°C) under short days. Application of 100 ppm benzylamino purine (BA) increased flower bud number by 40% when sprayed 2 weeks after the initiation of a short day treatment and increased phylloclade number up to 150% when applied during long day photo-periods.

In Vitro Propagation of Anigozanthos manglesii, Anigozanthos flavidus, and Macropidia fuliginosa1

Abstract Rapid in vitro propagation of Anigozanthos manglesii D. Don. was achieved through the initiation and proliferation of shoots from lateral bud explants using a modified Murashige and Skoog medium, or Gamborg, Miller and Ojima B5 medium, supplemented with 0.5 ppm benzylaminopurine (BA) and 0.5 ppm naphthaleneacetic acid (NAA). Continued proliferation was obtained by sub-culture either on the same media or media containing 0.25 ppm BA plus 0.25 ppm NAA. Rapid rooting of shoots occured following transfer to a half strength B5 medium containing 0.2 ppm NAA. Plantlets were successfully transplanted onto a University of California (UC) potting mix under mist and reduced light intensity. The method has also been successfully employed with Anigozanthos flavidus Redoute and with Macropidia fulginosa (Hook.) Druce.