Postharvest Quality Traits Research Articles

Understanding the Genetic Basis of Blueberry Postharvest Traits to Define Better Breeding Strategies.

Blueberry (Vaccinium spp.) is among the most-consumed soft fruit and has been recognized as an important source of health-promoting compounds. Highly perishable and susceptible to rapid spoilage due to fruit softening and decay during postharvest storage, modern breeding programs are looking to maximize quality and extend the market life of fresh blueberries. However, it is uncertain how genetically controlled postharvest quality traits are in blueberries. This study aimed to investigate the prediction ability and genetic basis of the main fruit quality traits affected during blueberry postharvest to create breeding strategies for developing cultivars with an extended shelf life. To achieve this goal, we carried out target genotyping in a breeding population of 588 individuals and evaluated for several fruit quality traits after one day, one week, three weeks, and seven weeks of postharvest storage at 1 °C. Using longitudinal genome-based methods, we estimated genetic parameters and predicted unobserved phenotypes. Our results showed large diversity, moderate heritability, and consistent predictive accuracies along the postharvest storage for most of the traits. Regarding fruit quality, firmness showed the largest variation during postharvest storage, with a surprising number of genotypes maintaining or increasing their firmness even after seven weeks of cold storage. Our results suggest that we can effectively improve blueberry postharvest quality through breeding and use genomic prediction to maximize the genetic gains in the long term. We also emphasize the potential of using longitudinal genomic prediction models to predict fruit quality at extended postharvest periods by integrating known phenotypic data from harvest.

Postharvest Quality of Citrus medica L. (cv Liscia-Diamante) Fruit Stored at Different Temperatures: Volatile Profile and Antimicrobial Activity of Essential Oils.

Citron (Citrus medica L. cv. Liscia-diamante), cultivated in the "Riviera dei Cedri" (southern Italy), is mainly utilized in the production of candied fruit and essential oils (EOs). Up to now, no information regarding the effect of storage temperatures on citron has been reported. Here, citron samples, after harvesting, were stored at different temperatures (5, 10 and 20 °C at 70% relative humidity) for two weeks, and the main postharvest quality parameters were evaluated. Moreover, EOs extracted from the stored samples were chemically characterized to reveal changes in the volatiles profile and antimicrobial activity. The EOs presented monoterpene hydrocarbons (87.1 to 96.3% of the total oil profile) as the most abundant compounds, followed by oxygenated metabolites ranging from 9.7 to 3.1% of the total pattern. Postharvest quality traits showed a good retention of green peel color during storage at 5 °C, while EOs from samples stored for 7 and 14 days at 10 and 20 °C, respectively, showed the highest antimicrobial activity against most assayed strains. The results indicated storage at 10 °C for 7 days as the most suitable for the preservation of the postharvest quality of the fruit and the antimicrobial activity of the extracted EOs.

Genetic architecture of post-harvest tuber quality traits in bush yam (Dioscorea praehensilis Benth.) germplasm through association mapping

IntroductionBush yam (Dioscorea praehensilis Benth.) is an important semi-domesticated food crop in West Africa. Limited information on the genetic architecture and its poor post-harvest tuber quality traits significantly hinder its use as food and source of income. Hence, dissecting the genetics underlying the expression of its post-harvest tuber quality traits is essential for establishing proper breeding schemes.MethodsIn this study, 138 D. praehensilis accessions collected in Ghana were sequenced using Diversity Array Technology (DArTSeq). The materials were profiled for dry matter content (DMC), tuber flesh oxidation (TBOXI) and for tuber flesh hardness (TBhard) during two cropping seasons.Results and discussionDiversity assessment using population structure, principal component analysis and hierarchical clustering methods revealed the presence of three major groups. Six genetic models were used for the trait association analysis using multiple random locus mixed linear model (MrMLM). Sixteen SNP markers distributed across the yam genome were identified to be associated with the evaluated traits. The associated SNP markers displayed a phenotypic variance ranged from 4.22% in TBHard to 16.92% in TBOXI. A total 25 putative candidate genes were identified around the SNP markers. The putative genes were identified to play key roles in tuber bulking, oxidative browning and starch hydroxylase. This study provides a valuable insight on the genetics underlying tuber quality traits in bush yam and opens avenues for developing genomic resources to improve D. praehensilis.

Definition of sensory and instrumental thresholds of acceptability for selection of cassava genotypes with improved boiling properties.

Consumers of boiled cassava in Africa, Latin America and Asia use specific preference criteria to evaluate its cooking quality, in terms of texture, colour and taste. To improve adoption rates of improved cassava varieties intended for consumption after boiling, these preference criteria need to be determined, quantified and integrated as post-harvest quality traits in the target product profile of boiled cassava, so that breeding programs may screen candidate varieties based on both agronomic traits and consumer preference traits. Surveys of various end-user groups identified seven priority quality attributes of boiled cassava covering root preparation, visual aspect, taste and texture. Three populations of contrasted cassava genotypes, from good-cooking to bad-cooking, in three countries (Uganda, Benin, Colombia) were then characterized according to these quality attributes by sensory quantitative descriptive analysis (QDA) and by standard instrumental methods. Consumers' preferences of the texture attributes mealiness and hardness were also determined. By analysis of correlations, the consumers' preferences scores were translated into thresholds of acceptability in terms of QDA scores, then in terms of instrumental measurements (water absorption during boiling and texture analysis). The thresholds of acceptability were used to identify among the Colombian and Benin populations promising genotypes for boiled cassava quality. This work demonstrates the steps of determining priority quality attributes for boiled cassava and establishing their corresponding quantitative thresholds of acceptability. The information can then be included in boiled cassava target product profiles used by cassava breeders, for better selection and adoption rates of new varieties. © 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Multivariate Analysis for Post Harvest Quality and Yield Attributes in Tomato (Solanum lycopersicum L.)

Aim: To study the multivariate analysis, genetic parameters and correlation for post harvest quality and yield traits in tomato.
 Study Design: The variability in the twenty genotypes of tomato (Solanum lycopersicum L.) was evaluated for 18 yield attributes and post harvest quality traits using randomized block design and analyzed with multivariate methods.
 Place and Duration of Study: Twenty genotypes of tomato augmented from Indian Institute of Vegetable Research, Varanasi were sown during rainy season at Horticulture Research Farm of Banaras Hindu University.
 Methodology: The unweighted pair group method of the average linkage (UPGMA) cluster analysis and principal component analysis (PCA) were used to analyze the data. Canonical discriminant analysis showed the contribution of each trait to the classification of the tomato accessions into different cluster groups.
 Results: The first seven principal components (PC) explained 87.83% of total variation and has eigen values >1. The traits that mainly contributed for this variation in PC1 and PC2 are fruit yield, plant height, number of flower clusters per plant, number of fruits per plant, days to first fruit set, number of fruits per cluster. High (>20%) genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) was observed for number of fruits/cluster, fruit shape index, number of fruits/plant, fruit yield and shelf life. The correlation coefficients of number of primary branches/plant, days to first fruit set, number of flower clusters/plant, number of fruits/cluster, fruit width (cm), number of fruits/plant, fruit weight (g) and lycopene (mg/100 g) were positively and significantly correlated to fruit yield/plant.
 Conclusion: Number of flower clusters/plant, fruit weight and number of fruits per plant had contributed for maximum variation. These traits also had high heritability, high genetic gain and significant correlation with fruit yield.

Genome-wide association identifies key loci controlling blackberry postharvest quality.

Blackberry (Rubus subgenus Rubus) is a soft-fruited specialty crop that often suffers economic losses due to degradation in the shipping process. During transportation, fresh-market blackberries commonly leak, decay, deform, or become discolored through a disorder known as red drupelet reversion (RDR). Over the past 50 years, breeding programs have achieved better fruit firmness and postharvest quality through traditional selection methods, but the underlying genetic variation is poorly understood. We conducted a genome-wide association of fruit firmness and RDRmeasured in 300 tetraploid fresh-market blackberry genotypes from 2019-2021 with 65,995 SNPs concentrated in genic regions of the R. argutus reference genome. Fruit firmness and RDR had entry-mean broad sense heritabilities of 68% and 34%, respectively. Three variants on homologs of polygalacturonase (PG), pectin methylesterase (PME), and glucan endo-1,3-β-glucosidase explained 27% of variance in fruit firmness and were located on chromosomes Ra06, Ra01, and Ra02, respectively. Another PG homolog variant on chromosome Ra02 explained 8% of variance in RDR, but it was in strong linkage disequilibrium with 212 other RDR-associated SNPs across a 23 Mb region. A large cluster of six PME and PME inhibitor homologs was located near the fruit firmness quantitative trait locus (QTL) identified on Ra01. RDR and fruit firmness shared a significant negative correlation (r = -0.28) and overlapping QTL regions on Ra02 in this study. Our work demonstrates the complex nature of postharvest quality traits in blackberry, which are likely controlled by many small-effect QTLs. This study is the first large-scale effort to map the genetic control of quantitative traits in blackberry and provides a strong framework for future GWAS. Phenotypic and genotypic datasets may be used to train genomic selection models that target the improvement of postharvest quality.

Postharvest Quality Traits of Chestnut (Castanea sativa Mill.) Fruit as Affected by Methyl Jasmonate During Cold Storage

Postharvest Quality Traits of Chestnut (Castanea sativa Mill.) Fruit as Affected by Methyl Jasmonate During Cold Storage

Pre and postharvest treatments of methyl jasmonate: Maintain quality and shelf-life of Kinnow mandarin fruit during cold storage

• Pre and Postharvest MeJA application maintained the quality of Kinnow mandarin fruit during cold storage. • 0.5 mM MeJA application reduced weight loss, retained higher fruit firmness, flavonoids, carotenoids, Vitamin C, total phenols, antioxidant activity, peroxidase enzyme activity. • 0.5 mM MeJA foliar application gives better results over postharvest treatment with regard to various desirable postharvest quality traits of Kinnow mandarin fruit. Kinnow mandarin fruit were subjected to pre and postharvest treatment with four concentrations (0.1, 0.3, 0.5 and 0.7 mM) of methyl jasmonate (MeJA). Treated fruits were stored at 6 °C and 85–95% relative humidity for 40 days (d). Comparatively, pre-harvest application, retained higher moisture content, fruit firmness and TSS/acidity ratio over the after harvest treated fruits on 40 days of storage. Moreover, preharvest harvest application of MeJa (0.5 mM) maintained 2.01-fold higher flavonoids and 1.40-fold higher Vitamin C over the control. An increase in total phenolic content, antioxidant activity and peroxidase enzyme activity were reported in pre-harvest treated fruit over postharvest treatment and control at the end of storage. The principal components analysis has also shown comparatively better response of pre-harvest MeJA application to most of the postharvest desirable fruit traits over postharvest treatments. From this study, it is concluded that pre-harvest MeJA (0.5 mM) application -40 days before harvesting is more effective over postharvest aqueous application.

Genome Editing Technology for Genetic Amelioration of Fruits and Vegetables for Alleviating Post-Harvest Loss.

Food security and crop production are challenged worldwide due to overpopulation, changing environmental conditions, crop establishment failure, and various kinds of post-harvest losses. The demand for high-quality foods with improved nutritional quality is also growing day by day. Therefore, production of high-quality produce and reducing post-harvest losses of produce, particularly of perishable fruits and vegetables, are vital. For many decades, attempts have been made to improve the post-harvest quality traits of horticultural crops. Recently, modern genetic tools such as genome editing emerged as a new approach to manage and overcome post-harvest effectively and efficiently. The different genome editing tools including ZFNs, TALENs, and CRISPR/Cas9 system effectively introduce mutations (In Dels) in many horticultural crops to address and resolve the issues associated with post-harvest storage quality. Henceforth, we provide a broad review of genome editing applications in horticulture crops to improve post-harvest stability traits such as shelf life, texture, and resistance to pathogens without compromising nutritional value. Moreover, major roadblocks, challenges, and their possible solutions for employing genome editing tools are also discussed.

Combining disease resistance and postharvest quality traits by early marker-assisted backcrossing in carioca beans

Common bean is a worldwide important crop. The development of varieties with durable resistance to diseases is a major challenge in common bean breeding. The present study aimed at evaluating the phenotypic and molecular selection of anthracnose resistance in a population obtained by assisted backcrossing from IAC Formoso (resistant, donor parent) × BRS Pérola (susceptible, recurrent parent). Nine microsatellites (SSRs) and one Sequence Tagged Sites (STS) markers previously linked to ANT resistance were used to genotype this progeny, and the results showed that the selection of the genotypes closest to the donor parent in the BC1F1 population decreased the number of backcrossing cycles necessary to obtain advanced isogenic lines, potentiating the use of this tool for early selection of resistant cultivars. A total of 31 % of the BC1F1 progeny was selected and backcrossed again. The progeny derived from the second backcross (BC2F3) was selected for the Carioca grain ideotype, and 42 % of the genotypes showed high resistance to anthracnose under controlled conditions of infection for races 65 and 81. Superior resistant plants were selected and evaluated under natural conditions of infection to fusarium wilt and angular leaf spot, allowing the selection of two inbred lines with higher resistance to anthracnose, fusarium wilt, angular leaf spot and postharvest quality traits such as yield, 100 seed weight, L value at seed harvest grain darkening and cooking time. The approach outlined in this paper proved to be effective to simultaneously select for disease resistance without losing technological quality aspects of the bean.

Fertilizer type and harvest maturity index on nutritive content and proximate composition of tomato fruit

ABSTRACT There may be a link between pre-harvest treatment and the post-harvest quality traits of tomato (Solanum lycopersicum L) fruit. The experiment was undertaken to determine the relationship between year of production, fertilizer type, and fruit-ripening index on nutritive and biochemical contents of tomato fruits sourced from the Directorate of University Farms, Federal University of Agriculture, Abeokuta, Nigeria. Data were collected on: proximate content, total soluble solids (TSS), Vitamin C, lycopene, pH and β-carotene. Fruits harvested at full ripe stage had the highest ash, moisture content, crude fiber, crude protein, fat, ß-carotene, TSS and Vitamin C contents. Year of production did not affect proximate content except for crude fiber. Fertilizer type did not affect proximate content except for ash content. Harvest maturity stage influenced proximate, nutritive, and biochemical content of tomato fruit. Fertilizer type used for cultivation and the stage of maturity at harvest are important factors determining tomato fruit nutritive contents and proximate composition. Knowledge of the right stage of maturity at harvest will affect supply of good quality tomato for the fresh market and food processing.

Sample size for postharvest quality traits of ‘Palmer’ mangoes

Abstract The objective of this study was to determine the most efficient sample size required to estimate the mean of postharvest quality traits of ‘Palmer’ mangoes harvested in two growing seasons. A total of 50 mangoes were harvested at maturity stage 2, in winter (June 2020) and spring (October 2020), and evaluated for weight, length, ventral and transverse diameter, skin and pulp L*, C* and hº, dry matter, firmness, soluble solids (SS), titratable acidity (TA) and the SS/TA ratio. According to the results, the coefficient of variation (CV) of fruit quality traits ranged from 2.1% to 18.1%. The highest CV in both harvests was observed for the SS/TA ratio, while the lowest was reported for pulp hº. In order to estimate the mean of physicochemical traits of ‘Palmer’ mangoes, 12 fruits are needed in the winter and 14 in the spring, considering an estimation error of 10% and a confidence interval of 95%. TA and the SS/TA ratio required the highest sample size, while L* and hº required the lowest sample size. In conclusion, the variability was different among physicochemical traits and seasons, implying that different sample sizes are required to estimate the mean of different quality traits in different growing seasons.

Innovative breeding technologies in lettuce for improved post-harvest quality.

Societal awareness of healthy eating is increasing alongside the market for processed bagged salads, which remain as one of the strongest growing food sectors internationally, including most recently from indoor growing systems. Lettuce represents a significant proportion of this ready-to-eat salad market. However, such products typically have a short shelf life, with decay of post-harvest quality occurring through complex biochemical and physiological changes in leaves and resulting in spoilage, food waste and risks to health. We review the functional and quantitative genetic understanding of lettuce post-harvest quality, revealing that few findings have translated into improved cultivar development. We identify (i) phytonutrient status (for enhanced antioxidant and vitamin status, aroma and flavour) (ii) leaf biophysical, cell wall and water relations traits (for longer shelf life) (iii) leaf surface traits (for enhanced food safety and reduced spoilage) and (iv) chlorophyll, other pigments and developmental senescence traits (for appearance and colour), as key targets for future post-harvest breeding. Lettuce is well-placed for rapid future exploitation to address postharvest quality traits with extensive genomic resources including the recent release of the lettuce genome and the development of innovative breeding technologies. Although technologies such as CRISPR/Cas genome editing are paving the way for accelerated crop improvement, other equally important resources available for lettuce include extensive germplasm collections, bi-parental mapping and wide populations with genotyping for genomic selection strategies and extensive multiomic datasets for candidate gene discovery. We discuss current progress towards post-harvest quality breeding for lettuce and how such resources may be utilised for future crop improvement.

Cytological changes and some postharvest quality traits of leeks grown in soil incorporated with partially burnt rice hull as a low-cost silicon source

Cytological changes and some postharvest quality traits of leeks grown in soil incorporated with partially burnt rice hull as a low-cost silicon source

Stakeholders’ Perception and Preferences of Post-harvest Quality Traits of Tomato in Ghana

The high levels of tomato consumption coupled with wide production levels in Ghana make the crop economically viable. Food preferences vary among individuals and geographical locations. Traits to select during crop improvement therefore, depend on the target beneficiaries. Breeders sometimes fail to consider preferences of end users probably because they are oblivious of them. This study used Participatory Rural Appraisal (PRA) and surveys to identify preferences and perceptions of end users in tomato value chain, for making proper breeding choices according to the information collected. The strategy employed for this research was descriptive survey. The target population included tomato farmers, market players and consumers. A multi-stage sampling was used to select the study sample. More than half (57.3%) of the respondents had been involved in tomato production for over 10 years. Sixteen variables were discussed with each group. The first three principal components (PCs) with Eigen values greater than 1.0 together explained 100% of total variation in the data set. Scores for ranking popular vegetable and ranking causes for poor shelf life were not significantly associated with any of the components. The Focus Group Discussion (FGDs) also established that, seven main vegetable crops were cultivated and produced by the communities and ranked tomato as number one. Similarly, six important quality traits of tomato were mentioned by stakeholders as their favorite. Respondents showed extreme preferences for firmness and shelf life as the most important quality traits of tomato. Seventy-seven-point five percent (77.5%) of the consumers use tomatoes every day in their food preparation. The study revealed stakeholders’ desire and plea for firmness and extended shelf life of tomato fruits in Ghana. Consequently, the survey validated the need to regulate tomato breeding goals to develop high yielding tomatoes with improved fruit quality and prolonged shelf life.

Combining ability and gene action studies of some post-harvest and nutritional quality traits in tomato (Solanum lycopersicum L)

Combining ability and gene action studies of some post-harvest and nutritional quality traits in tomato (<i>Solanum lycopersicum</i> L)

Fruit color and post-harvest shelf life in tomato affected by the ogc, nor A, and rin alleles

Abstract: The objective of this work was to evaluate the effects of the mutant alleles alcobaça (nor A ), ripening inhibitor (rin), and old gold crimson (og c ), in heterozygosity or homozygosity, on the expression of color and on the postharvest quality of fruit of experimental tomato hybrids. Fourteen hybrids with contrasting genotypic constitutions in the nor A , rin, and og c loci were evaluated in a randomized complete block design with four replicates. The following fruit postharvest quality traits were evaluated: firmness in the breaker stage, color, and soluble solids content. The rin +/rin and nor +/nor A genotypes increased firmness of tomato fruit at harvest (breaker stage). The rin + /rin genotypes displayed the worse internal fruit color. There was a positive effect of og c+ /og c in improving the internal color of rin + /rin and nor + /nor A fruit, making the color similar to that of the normal genotypes. The combination of the og c /og c rin + /rin nor + /nor A genes is effective to improve tomato fruit firmness, besides maintaining or improving internal color.

Effects of 1-Methylcyclopropene on postharvest quality traits, antioxidant activity and ascorbic acid content of mature-ripe mango fruits

Effects of 1-Methylcyclopropene on postharvest quality traits, antioxidant activity and ascorbic acid content of mature-ripe mango fruits

Genetic divergence among processing tomato hybrids and formation of new segregating populations

ABSTRACT Tomato is the most important vegetable species and has a strong bottleneck effect in its domestication and evolution. In exploring the existing genetic variability in commercial germplasm, germplasm has been proven to be an excellent alternative to obtain inbred lines in order to provide superior new hybrids in the future. In this sense, the objective of this study was to estimate the genetic distance among commercial processing tomato hybrids via agronomical and quality postharvest fruit traits with the aim of suggesting promising crosses for the formation of base populations for tomato breeding. Ten hybrids of processing tomato were evaluated in a complete randomized block design with three replicates. In total, eleven agronomic and postharvest fruit quality traits were evaluated. The genetic distances were estimated between the hybrids using the generalized Mahalanobis () and Gower () distances. The genetic distance among tomato hybrids was determined using a graphic projection of the first two canonical variables. The presence of significant genetic variability among the hybrids (P &lt;0.05) was demonstrated and was sufficient for the selection of the best hybrids before the breeding process. The hybrid Laura stood out for its postharvest characteristics and was the most divergent genotype compared to the others evaluated. The most promising crossings for the formation of segregating populations with superior genetic merit are Kátia x Laura, Vênus x Laura, Fascínio x Laura, AP-533 x Laura, Tinto x Laura, AP-529 x Laura, Supera x Laura, Granadero x Laura, Granadero x AP533, Granadero x Ap529 and Granadero x Kátia.

Postharvest and Sensory Evaluation of Selected ‘Hass’ × ‘Bacon’ and ‘Bacon’ × ‘Hass’ Avocado Hybrids Grown in East-Central Florida

Avocado (Persea americana Mill.) is a high-value fruit that continues to increase in consumer demand. A population of ‘Hass’–‘Bacon’ hybrids was planted at USDA-ARS, Fort Pierce, as part of a study to find selections with good horticultural and postharvest quality traits for Florida. Extensive phenotypic data on quality were collected over 3 years. Ten selections were identified in 2014 and 2015 with promising fruit quality and postharvest shelf life characteristics and were tested in sensory panels using store-bought ‘Hass’ as the standard. In general, the selections had fruit quality similar to commercial ‘Hass’. Avocados that were most liked were described as creamy in texture with buttery and nutty flavor. Only one selection (R7T54 in 2014) and one store-bought control (‘Hass’ in 2015) were disliked, which was associated with greater firmness at the time of evaluation, likely relating to insufficient postharvest conditioning. Furthermore, CA ‘Hass’ commercial requirements for minimum dry matter (20.8%) were generally achieved by these selections under Florida conditions, ranging from 18.4% to 25.7%. This study identified 10 selections with composition and sensory quality similar to ‘Hass’ that are suitable for further testing and development in Florida.