Pore Size Gradient Research Articles

Experimental Study on the Effect of Carbon Graphitization Degree and Pore Structure on the Electrochemical Durability of Gas Diffusion Layers

Gas diffusion layers (GDLs) in high-temperature, high-humidity, and high-electric-potential environments can be affected by the carbon corrosion and degradation of Polytetrafluoroethylene (PTFE) network structures, resulting in reduced reliability and hydrophobicity. By using cyclic voltammetry and offline characterization, a high-potential scanning of 1–1.5 V is applied to the GDL in the three-electrode system, considering the role of gradient graphitization degree and pore size structure in corrosion. Accelerating the electrochemical corrosion process of carbon and PTFE allows the identification of corrosion location, extent, and determinants. The results indicate that after 800 cycles of high-potential triangulation scanning, the graphitization of gas diffusion base has the most significant impact on the GDL’s durability. On the other hand, the durability of the GDL’s microporous layer is influenced by its small pore size structure rather than its graphitization degree. Furthermore, the corrosion process of GDLs with a small pore size structure tends to be relatively slow, providing a basis for GDL selection and durability prediction.

Continuous printing of bone-inspired hierarchical porous objects using video projection-based stereolithography

Bone-inspired porous structures have attracted intense attention in biomedical and mechanical applications due to their unique combination of strength, toughness, and low weight. Although layer-by-layer-based additive manufacturing techniques can build structures with high geometric complexity, it remains challenging to fabricate porous objects with gradient porosity and pore size ranging from nano- to micro-scale. This research investigates the video projection-based stereolithography (VP-SL) process, which is a continuous 3D printing process, also known as Continuous Liquid Interface Production (CLIP), for fabricating hierarchical porous structures with nano-, micro- and macro-scale pores and surface features. This work tested the hypothesis that tuning the printing speed during the VP-SL process can generate an imbalance between curing and resin recoating, resulting in pores in cured polymer. First, the VP-SL process, setup, and the mechanisms of forming micropores and nanowrinkles were introduced. Then, the printing range of the VP-SL process was characterized using curing depth experiments. To validate the efficiency and feasibility of the proposed approach, various three-dimensional hierarchical structures were fabricated with homogeneous and gradient porosity, and the effect of porosity distribution on mechanical properties was studied. Test cases were printed and characterized to validate the feasibility and efficiency of the proposed approach. The results demonstrated the capability of the VP-SL process and its potential in the production of gradient porous objects for various applications, such as scaffolds for custom bone implants.

Influence of structural parameters of 3D-printed triply periodic minimal surface gyroid porous scaffolds on compression performance, cell response, and bone regeneration.

In this study, multi-scale triply periodic minimal surface (TPMS) porous scaffolds with uniform and radial gradient distribution on pore size were printed based on the selective laser melting technology, and the influences of porosity, pore size and radial pore size distribution on compression mechanical properties, cell behavior, and bone regeneration behavior were analyzed. The results showed that the compression performance of the uniform porous scaffolds with high porosity was similar to that of cancellous bone of pig tibia, and the gradient porous scaffolds have higher elastic modulus and compressive toughness. After 4 days of cell culture, cells were distributed on the surface of scaffolds mostly, and the number of adherent cells was higher on the small pore size porous scaffolds; After 7 days, the area and density of cell proliferation on the scaffolds were improved; After 14 days, the cells on the small pore size scaffolds tended to migrate to adjacent pores. Animal implantation experiments showed that collagen fiber osteoid was intermittent on scaffolds with high porosity and large pore size, which was not conducive to bone formation. The appropriate pore size and porosity of bone regeneration were 792 um and 83%, respectively, and the regenerative ability of gradient pore size was better than that of uniform pore size. Our study explains the rules of TPMS gyroid structure parameters on compression performance, cell response and bone regeneration, and provides a reference value for the design of bone repair scaffolds for clinical orthopedics.

An improved vapor chamber with enhanced two-phase transport by using structured surfaces

The novel type of vapor chamber (VC) with combined superhydrophilic/superhydrophobic surface holds great potential in hot spot cooling. In this study, an improved VC was developed by implementing structured surfaces on both condenser and evaporator. The thermal resistance, condenser temperature uniformity, and gravity independence were discussed. It was found that sintering an appropriate copper mesh on condenser could reduce the thermal resistance but would cause a slight deterioration in the temperature uniformity of the condenser. On the evaporator, the thermal performance of VC was further improved by introducing micro grooves on the wick in order to enhance bubble discharge of the boiling working fluid. Moreover, to strengthen the liquid recirculation on the evaporator, pore size gradient was adopted in the evaporator wick. Compared to the axial gradient of pore size, the radial gradient of pore size was more efficient in accelerating the working fluid recirculation. Among all the results reported in this study, the combination of sintering copper mesh on the condenser and implementing micro-grooves and pore size gradient on the evaporator wick yields optimal results, with the minimum thermal resistance of the VC reaching 0.041 K/W and the critical heat flux exceeding 213.3 W/cm2, which shows excellent thermal performance.

Experimental Investigation on Thermal Performance of Vapor Chambers with Diffident Wick Structures

In this paper, a type of vapor chamber with a gradient pore size wick (VC-G) was developed, and its thermal performance was experimentally tested and compared with two types of VCs with uniform pore size wick (which can be defined as VC-U (200) and VC-U (50) as the powder size of the wick is 50-mesh and 200-mesh, respectively) and a VC without a wick (VC-N). In addition, a VC heat transfer ability experiment platform was built, and the thermal resistance, temperature distribution and thermal response time of the VC with different wick structures were experimentally investigated. The experiment results show that the capillary driving force provided by gradient pore size wick increases gradually from outside, which can not only promote the condensation fluid to gather in the central heat source but also facilitate the vapor to spread around. Therefore, compared with VC-U (200), VC-U (50) and VC-N, VC-G shows the best heat transfer performance, temperature uniform performance and start-up performance.

The effect of particle size on the sublimation behavior of butylhydroxytoluene as antioxidant in tablets during storage and coating

The effect of particle size on the sublimation behavior of butylhydroxytoluene (BHT) was investigated when BHT was included as antioxidant in tablets. Sublimation of pure BHT was found to be independent of its particle size, with pore formation on the surface of all tablets after storage at room temperature and above. Moreover, a higher residual BHT content after storage was detected in tablets containing a larger size fraction. X-ray µCT scans revealed the formation of peripherally larger pores at higher BHT particle sizes, implying a slower sublimation rate in the tablet core. A stability study indicated an increase in the extent of BHT sublimation at higher temperature and longer exposure time for all size fractions. The influence of BHT particle size was more pronounced when the tablets were stored at higher temperature, but the effect receded with longer exposure time. Similar trends were seen in film-coated tablets. Due to the short exposure time to elevated temperatures, a gradient in pore size was also observed at smaller particle sizes, with peripheral pores being larger in uncoated tablets. Superficial pores disappeared when a film coating was deposited onto the tablets. After storage of the film-coated tablets, less BHT had sublimated compared to the uncoated tablet. The coating layer did not prevent sublimation, but the process was slowed down.

Machine learning, experiments and molecular simulation demonstrate the adsorption mechanism of acetone on porous carbon at different pressures

The scientific design and preparation of porous carbon with high VOCs (such as acetone) adsorption capacity are crucial for waste gas treatment. However, it is still challenging to explain the microscopic mechanism of acetone uptake on porous carbon because of the different adsorption conditions, as well as various chemical properties and different pore structures of porous carbon. Here, the prediction model of the adsorption conditions, nitrogen/oxygen groups and pore structures on acetone adsorption capacity was established by machine learning, and the contribution of the nitrogen/oxygen groups and pore structures of porous carbons to the acetone adsorption capacity at different adsorption pressures was discussed. The results display that the nitrogen/oxygen groups and micropore volume are the main factors affecting the adsorption capacity of acetone at relatively low pressure, while the adsorption capacity at relatively high pressure is determined by total pore volume. Subsequently, three kinds of porous carbon with different oxygen content and gradient pore size distribution were synthesized, and acetone adsorption isotherms were tested. The effects of chemical properties and pore structure on acetone adsorption at different pressure were studied, and the results were consistent with those of machine learning. However, the results are challenging to illustrate the effect of single pore size and functional group type on acetone adsorption performance. Finally, molecular simulation was used to calculate acetone adsorption isotherms with various nitrogen/oxygen groups and different pore sizes, further revealing the adsorption mechanism of acetone with nitrogen/oxygen groups and pore size. Based on machine learning and molecular simulation results, new insights into the adsorption behavior of acetone were revealed, providing theoretical support for the sustainable development of carbon-based adsorbents for acetone waste gas treatment.

Two-Stage Microporous Layers with Gradient Pore Size Structure for Improving the Performance of Proton Exchange Membrane Fuel Cells.

In this paper, we report the preparation of a gas diffusion layer (GDL) with different gradient pore size structures. The pore structure of microporous layers (MPL) was controlled by the amount of pore-making agent sodium bicarbonate (NaHCO3). We investigated the effects of the two-stage MPL and the different pore size structures in the two-stage MPL on the performance of proton exchange membrane fuel cells (PEMFC). The conductivity and water contact angle tests showed that the GDL had outstanding conductivity and good hydrophobicity. The results of the pore size distribution test indicated that introducing a pore-making agent altered the pore size distribution of the GDL and increased the capillary pressure difference within the GDL. Specifically, there was an increase in pore size within the 7-20 μm and 20-50 μm ranges, which improved the stability of water and gas transmission within the fuel cell. The maximum power density of the GDL03 was increased by 37.1% at 40% humidity, 38.9% at 60% humidity, and 36.5% at 100% humidity when compared to the commercial GDL29BC in a hydrogen-air environment. The design of gradient MPL ensured that the pore size between carbon paper and MPL changed from an initially abrupt state to a smooth transition state, which significantly improved the water and gas management capabilities of PEMFC.

3D printed PCL/nHAp scaffolds: Influence of scaffold structural parameters on osteoblast performance in vitro

AbstractScaffolds play a key role in bone tissue engineering (BTE) as they provide a mechanically and biologically supportive template to treat bone defects. Yet, the ideal scaffold structures are far from certain, leaving a lot to be discovered in terms of the scaffold structure–performance relationships. In this study, we investigated the influence of pore size and internal structure on osteoblast performance in vitro. Three‐dimensional (3D) scaffolds were printed from polycaprolactone (PCL) reinforced with 30% (wt.) nano‐hydroxyapatite (nHAp), with two different internal structures (lattice and staggered) and four pore sizes (0.280, 0.380, 0.420, and 0.550 mm). Scaffolds were seeded with pre‐osteoblast cells (MC3T3‐E1). Metabolic activity of cells, osteoblast differentiation, and capability of osteoblasts to deposit mineralized matrix were examined in vitro. Staggered scaffolds better supported cellular performance. The pore size of 0.280 mm was more favorable to support cell proliferation while the pore size ≥0.420 mm was more effective to promote osteoblast differentiation and mineralization. Findings revealed that osteoblast activities were affected differently by the pore size. Our study further suggests that the structure with a gradient pore size would be better than a single size in terms of supporting cell proliferation, differentiation, and secretion of a mineralized matrix.

Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure.

Titanium alloy scaffolds with a porous structure have attracted much attention in bone defect repair. However, which pore structure is more beneficial to bone defect repair is controversial. In the present research, the Ti6Al4V alloy porous scaffolds with gradient pore sizes were designed and fabricated. The microstructure characterization, tests of mechanical properties, and in vitro and in vivo experiments have been performed to systematically evaluate the effect of pore size on osteoinduction and osteogenesis. The results revealed that the contact angle with water, compressive strength, and elastic modulus of the Ti6Al4V alloy porous scaffolds decreased gradually with the increase of pore size. However, there were obvious drops when the pore size of the porous scaffold was around 600μm. As the pore size increased, the proliferation and integrin β1 of RAW 264.7 macrophages seeded on Ti6Al4V alloy porous scaffolds increased at first, reaching a maximum value at a pore size of around 600μm, and then decreased subsequently. The proliferation, integrin β1, and osteogenic gene-related expressions of Bone marrow mesenchymal stem cells (BMSCs) seeded on Ti6Al4V alloy porous scaffolds with different pore sizes all exhibited similar variations which rose with increased pore size firstly, obtaining the maximum value at pore size about 600μm, and then declined. The in vivo experiments confirmed the in vitro results, and the Ti6Al4V alloy porous scaffold with a pore size of 600μm possessed the better capability to induce new bone formation. Therefore, for the design of Ti6Al4V alloy with a regular porous scaffold, the surface morphology, porosity, strength, and elastic modulus should be considered systematically, which would determine the capability of osteoinduction and osteogenesis.

Effect of protein fouling on filtrate flux and virus breakthrough behaviors during virus filtration process.

Virus filtration process is used to ensure viral safety in the biopharmaceutical downstream processes with high virus removal capacity (i.e., >4 log10 ). However, it is still constrained by protein fouling, which results in reduced filtration capacity and possible virus breakthrough. This study investigated the effects of protein fouling on filtrate flux and virus breakthrough using commercial membranes that had different symmetricity, nominal pore size, and pore size gradients. Flux decay tendency due to protein fouling was influenced by hydrodynamic drag force and protein concentration. As the results of prediction with the classical fouling model, standard blocking was suitable for most virus filters. Undesired virus breakthrough was observed in the membranes having relatively a large pore diameter of the retentive region. The study found that elevated levels of protein solution reduced virus removal performance. However, the impact of prefouled membranes was minimal. These findings shed light on the factors that influence protein fouling during the virus filtration process of biopharmaceutical production.

Green-Solvent-Processable Composite Micro/Nanofiber Membrane with Gradient Asymmetric Structure for Efficient Microfiltration.

Electrospinning technology has attracted extensive attention in recent decades and is widely used to prepare nanofiber membranes from hundreds of polymers. Polyvinyl formal acetal (PVFA), as a polymer with excellent properties such as high strength and heat resistance, is not reported on the electrospun water treatment membrane. In this paper, the preparation process of electrospun PVFA nanofiber membrane is optimized, and the effect of sodium chloride (NaCl) addition on the physical and mechanical properties and microfiltration performance of nanofiber membrane is also explored. And the hydrophobic PVFA nanofiber filter layer is then combined with a hydrophilic nonwoven support layer to construct a composite micro/nanofiber membrane with a pore-size gradient structure and a hydrophilic/hydrophobic asymmetric structure. Finally, unidirectional water transport and water treatment performance are further investigated. The results show that the tensile breaking strength of the composite membrane can reach up to 37.8 MPa, the retention rate for particles with the size of 0.1-0.3 µm is 99.7%, and the water flux is 513.4 L m-2 h-1 under the hydrostatic pressure. Moreover, it still has a retention of more than 98% after three repeated uses. Therefore, the electrospun PVFA composite membrane has a great potential in microfiltration.

FLIM imaging revealed spontaneous osteogenic differentiation of stem cells on gradient pore size tissue-engineered constructs

BackgroundThere is an urgent clinical need for targeted strategies aimed at the treatment of bone defects resulting from fractures, infections or tumors. 3D scaffolds represent an alternative to allogeneic MSC transplantation, due to their mimicry of the cell niche and the preservation of tissue structure. The actual structure of the scaffold itself can affect both effective cell adhesion and its osteoinductive properties. Currently, the effects of the structural heterogeneity of scaffolds on the behavior of cells and tissues at the site of damage have not been extensively studied.MethodsBoth homogeneous and heterogeneous scaffolds were generated from poly(L-lactic acid) methacrylated in supercritical carbon dioxide medium and were fabricated by two-photon polymerization. The homogeneous scaffolds consist of three layers of cylinders of the same diameter, whereas the heterogeneous (gradient pore sizes) scaffolds contain the middle layer of cylinders of increased diameter, imitating the native structure of spongy bone. To evaluate the osteoinductive properties of both types of scaffold, we performed in vitro and in vivo experiments. Multiphoton microscopy with fluorescence lifetime imaging microscopy was used for determining the metabolic states of MSCs, as a sensitive marker of cell differentiation. The results obtained from this approach were verified using standard markers of osteogenic differentiation and based on data from morphological analysis.ResultsThe heterogeneous scaffolds showed improved osteoinductive properties, accelerated the metabolic rearrangements associated with osteogenic differentiation, and enhanced the efficiency of bone tissue recovery, thereby providing for both the development of appropriate morphology and mineralization.ConclusionsThe authors suggest that the heterogeneous tissue constructs are a promising tool for the restoration of bone defects. And, furthermore, that our results demonstrate that the use of label-free bioimaging methods can be considered as an effective approach for intravital assessment of the efficiency of differentiation of MSCs on scaffolds.

Performance of polymer electrolyte fuel cells with gradient pore microporous layers at moderate/high humidity

In this paper, carbon black and acetylene black are used as conductive materials of microporous layer (MPL) in GDL, and four MPLs with different materials and structures are prepared by spray method. Respectively, GDL prepared using carbon black, acetylene black, blend of carbon black and acetylene black, double-microporous layer structure are used. In double MPLs, MPL near carbon paper side is acetylene black, and MPL near catalyst layer side is carbon black. The polarization curve, power density, electrochemical impedance spectroscopy, water permeability, pore size distribution and water contact angle of the four samples are tested. It indicated that GDL with double MPL structure has maximum current and power density. When humidity is 60%, limiting current density increased by 41%, and power density increased by 30% (Relative to GDL without gradient pore size). When humidity is 100%, limiting current density increased by 41.3%, power density increased by 50% (Relative to GDL without gradient pore size).

Influence of pore size distribution on pool boiling heat transfer in porous artery structure

To enhance the boiling heat transfer (BHT) performance is of great significance for high heat flux heat dissipation. Through effective liquid/vapor separation mechanism, porous artery structures are proved to significantly enhance the BHT performance. However, such structures are mainly focused on adjusting the liquid/vapor distribution on the macroscale, and the engineering of liquid/vapor distribution inside the porous structure is still missing. To facilitate the vapor escape and also the liquid replenishment inside the porous structure, porous artery structures with four types of pore size distribution, i.e., mono-porous, bi-porous, gradient-aperture, and gradient aperture and bi-porous distributions, were fabricated and experimentally investigated. Experimental results show that porous artery structures with both bi-porous and gradient-aperture distributions exhibit superior heat transfer performance compared to that with mono-porous distribution due to i) reduced liquid and vapor flow resistance; ii) increased liquid replenishment via capillary suction; and iii) efficient liquid/vapor phase separation. The maximum heat flux reached up to 554 W/cm2 for the optimized bi-porous distribution, which is increased by 269% compared to that on a smooth copper surface. In addition, the effects of particle size and the amount of additive PMMA on the BHT performance are investigated, and the underlying physical mechanisms are discussed.

Enhancing the performance of proton exchange membrane fuel cell using nanostructure gas diffusion layers with gradient pore structures

The gas diffusion layer (GDL) plays a vital role in water management of proton exchange membrane fuel cells. In this study, GDLs with nanoscale gradient pore sizes (nano-GDLs) are fabricated using electrospinning to explore the influence of the gradient layer number and degree of the pore size gradient on the water transport performance. The breakthrough pressure and water saturation tests indicate that nano-GDLs with uniform and gradient structures have higher breakthrough pressures and lower water saturation compared with those of the commercial Toray GDL, which facilitates back diffusion of water through the membrane and water discharge in the GDL. Specifically, among the nano-GDLs with a gradient structure, the three-layer medium-gradient nano-GDL sample (3-MG) possesses the maximum breakthrough pressure and minimum water saturation ratio. The fuel cell test determined that the maximum power density of the 3-MG nano-GDL sample is 14% higher than that with commercial Toray GDL without cathode humidification.

Visualization of porosity and pore size gradients in electrospun scaffolds using laser metrology.

We applied a recently developed method, laser metrology, to characterize the influence of collector rotation on porosity gradients of electrospun polycaprolactone (PCL) widely investigated for use in tissue engineering. The prior- and post-sintering dimensions of PCL scaffolds were compared to derive quantitative, spatially-resolved porosity 'maps' from net shrinkage. Deposited on a rotating mandrel (200 RPM), the central region of deposition reaches the highest porosity, ~92%, surrounded by approximately symmetrical decreases to ~89% at the edges. At 1100 RPM, a uniform porosity of ~88-89% is observed. At 2000 RPM, the lowest porosity, ~87%, is found in the middle of the deposition, rebounding to ~89% at the edges. Using a statistical model of random fiber network, we demonstrated that these relatively small changes in porosity values produce disproportionately large variations in pore size. The model predicts an exponential dependence of pore size on porosity when the scaffold is highly porous (e.g., >80%) and, accordingly, the observed porosity variation is associated with dramatic changes in pore size and ability to accommodate cell infiltration. Within the thickest regions most likely to 'bottleneck' cell infiltration, pore size decreases from ~37 to 23 μm (38%) when rotational speeds increased from 200 to 2000 RPM. This trend is corroborated by electron microscopy. While faster rotational speeds ultimately overcome axial alignment induced by cylindrical electric fields associated with the collector geometry, it does so at the cost of eliminating larger pores favoring cell infiltration. This puts the bio-mechanical advantages associated with collector rotation-induced alignment at odds with biological goals. A more significant decrease in pore size from ~54 to ~19 μm (65%), well below the minimum associated with cellular infiltration, is observed from enhanced collector biases. Finally, similar predictions show that sacrificial fiber approaches are inefficient in achieving cell-permissive pore sizes.

Influence of structural features in the performance of bioceramic-based composite scaffolds for bone engineering applications: A prediction study

Despite recent years' remarkable progress in medical procedures for bone reconstruction, the implementation of newly developed biomedical materials is still facing technical drawbacks, such as, lack of mechanical strength, impaired cellular proliferation and differentiation, limited osteogenesis and issues related to bone infection, osseointegration and vascularization in large defects.This paper presents a methodology to predict the potential behaviour of bioceramic-based composite scaffolds for bone engineering applications, based on the evaluation of its morphological features, mechanical properties, and mechanical stimulus' input to cells. Chitosan/biphasic calcium phosphates (CH/BCP) composite scaffolds with different structural parameters, namely pore size, filament thickness and layer overlapping, pore size gradient and irregularity on the filament positioning were considered. The finite element simulation was validated against experimental results on 3D printed scaffolds, being proposed an in silico model for the characterization of the scaffolds' material composition. With this study, the influence of the CH/BCP bulk material mechanical properties on the scaffolds mechanical behaviour was demonstrated. CH/BCP show to behave according to a Yeoh hyperelastic material model. Scaffolds with a regular structure and 500 μm filament distance (PL500) or irregular architecture models (IS2 scaffold) demonstrated better equilibrium among the analysed features, namely to promote cell adhesion and migration, nutrient and oxygen flow, vascularization, mechanical capacity to support the load without experiencing premature failure and initial bone formation through the transmission of bone growth mechanical stimulus. A good correlation between this study and literature data was obtained, which highlighted the suitability of the proposed methodology to infer on the scaffold biomechanical behaviour and allowing the scaffold improvement at an early stage of conceptualization.

Hydrogel-Bondable Asymmetric Planar Membranes with Hierarchical Pore Structures for Cell Scaffolding and Encapsulation.

Biomaterials for cell replacement therapy could facilitate the delivery, function, and retrieval of transplanted therapeutic cells. However, the limited capacity to accommodate a sufficient quantity of cells in biomedical devices has hindered the success of clinical application, resulting from the suboptimal spatial organization of cells and insufficient permeation of nutrients in the materials. Herein, through the immersion-precipitation phase transfer (IPPT) process from polyether sulfone (PES), we develop planar asymmetric membranes with a hierarchical pore architecture spanning from nanopores (∼20 nm) in the dense skin and open-ended microchannel arrays with gradient pore size increasing vertically from microns to ∼100 μm. The nanoporous skin would be an ultrathin diffusion barrier, while the microchannels could support high-density cell loading by acting as separate chambers allowing uniform distribution of cells in the scaffold. Alginate hydrogel could permeate into the channels and form a sealing layer after gelation, which could slow down the invasion of host immune cells into the scaffold. The hybrid thin-sheet encapsulation system (∼400 μm thick) could protect allogeneic cells over half-year after intraperitoneal (IP) implantation in immune-competent mice. Such structural membranes and plastic-hydrogel hybrids of thin dimensions could find important applications in cell delivery therapy.

A flexible design framework to design graded porous bone scaffolds with adjustable anisotropic properties

Since the success of bone regenerative medicine depends on scaffold morphological and mechanical properties, numerous scaffolds designs have been proposed in the last decade, including graded structures that are suited to enhance tissue ingrowth. Most of these structures are based either on foams with a random pore definition, or on the periodic repetition of a unit cell (UC). These approaches are limited by the range of target porosities and obtained effective mechanical properties, and do not permit to easily generate a pore size gradient from the core to the periphery of the scaffold. In opposition, the objective of the present contribution is to propose a flexible design framework to generate various three-dimensional (3D) scaffolds structures including cylindrical graded scaffolds from the definition of a UC by making use of a non-periodic mapping. Conformal mappings are firstly used to generate graded circular cross-sections, while 3D structures are then obtained by stacking the cross-sections with or without a twist between different scaffold layers. The effective mechanical properties of different scaffold configurations are presented and compared using an energy-based efficient numerical method, pointing out the versatility of the design procedure to separately govern longitudinal and transverse anisotropic scaffold properties. Among these configurations, a helical structure exhibiting couplings between transverse and longitudinal properties is proposed and permits to extend the adaptability of the proposed framework. In order to investigate the capacity of common additive manufacturing techniques to fabricate the proposed structures, a subset of these configurations is elaborated using a standard SLA setup, and subjected to experimental mechanical testing. Despite observed geometric differences between the initial design and the actual obtained structures, the effective properties are satisfyingly predicted by the proposed computational method. Promising perspectives are offered concerning the design of self-fitting scaffolds with on-demand properties depending on the clinical application.