Porcine Model Of Injury Research Articles

Creating an Educational Program in the Endovascular and Hybrid Intervention; Experiences from the Japanese Society of Diagnostic and Interventional Radiology in Emergency, Critical Care, and Trauma (DIRECT)

Diagnostic and interventional radiology (IR) techniques have recently shown significant efficacy in trauma care when combined with surgical procedures. A multifaceted approach to traffic accidents has reduced the number of trauma patients in Japan, which has necessitated simulation education to provide practical experience and ensure proficiency. The objective of this paper is to report the educational development of endovascular and hybrid workshops in Japan. The Japanese Society of Diagnostic and Interventional Radiology in Emergency, Critical care, and Trauma (DIRECT) was established in 2011 to maximize the benefit of diagnostic and IR in emergency or trauma settings. DIRECT conducts trauma endovascular workshops for emergency medicine physicians, acute care surgeons, and IR physicians. From July 2011 to June 2016, DIRECT has conducted 14 simulator workshops, six endovascular workshops using a porcine model, and four hybrid trauma management workshops, of which two were conducted with porcine models. The simulation workshop was designed for novice learners and included resuscitative endovascular balloon occlusion of the aorta (REBOA) deployment using a pressurized silicone model, catheter and guidewire manipulation with a three-dimensional (3D) vessel silicone model and virtual fluoroscopic simulator, and metallic coil deployment. Porcine simulations for intermediate-level physicians were conducted and endovascular tools included different occlusion materials. In the hybrid strategy model, by using a porcine model of injury, the participants combined both surgical and endovascular procedures such as REBOA, selective balloon occlusion, and N-butyl cyanoacrylate embolization. DIRECT is a vital bridge between emergency/trauma and IR, and workshops are essential for improved trauma care.

Ductular and proliferative response of esophageal submucosal glands in a porcine model of esophageal injury and repair.

Esophageal injury is a risk factor for diseases such as Barrett's esophagus (BE) and esophageal adenocarcinoma. To improve understanding of signaling pathways associated with both normal and abnormal repair, animal models are needed. Traditional rodent models of esophageal repair are limited by the absence of esophageal submucosal glands (ESMGs), which are present in the human esophagus. Previously, we identified acinar ductal metaplasia in human ESMGs in association with both esophageal injury and cancer. In addition, the SOX9 transcription factor has been associated with generation of columnar epithelium and the pathogenesis of BE and is present in ESMGs. To test our hypothesis that ESMGs activate after esophageal injury with an increase in proliferation, generation of a ductal phenotype, and expression of SOX9, we developed a porcine model of esophageal injury and repair using radiofrequency ablation (RFA). The porcine esophagus contains ESMGs, and RFA produces a consistent and reproducible mucosal injury in the esophagus. Here we present a temporal assessment of this model of esophageal repair. Porcine esophagus was evaluated at 0, 6, 18, 24, 48, and 72 h and 5 and 7 days following RFA and compared with control uninjured esophagus. Following RFA, ESMGs demonstrated an increase in ductal phenotype, echoing our prior studies in humans. Proliferation increased in both squamous epithelium and ESMGs postinjury with a prominent population of SOX9-positive cells in ESMGs postinjury. This model promises to be useful in future experiments evaluating mechanisms of esophageal repair.NEW & NOTEWORTHY A novel porcine model of injury and repair using radiofrequency ablation has been developed, allowing for reproducible injury to the esophagus to study repair in an animal model with esophageal submucosal glands, a key anatomical feature and missing in rodent models but possibly harboring progenitor cells. There is a strong translational component to this porcine model given the anatomical and physiological similarities between pigs and humans.