Polyherbal Formulation Research Articles

Polyherbal Syrup for Coronavirus Infection: Formulation and Evaluation

Background: SARS-CoV-2 emerged in Wuhan in December 2019, and after that, it spread quickly around the world. The virus could spread to millions of individuals since there were no particular treatments or preventative measures. The COVID-19 infection is often treated with current drugs such as Remdesivir, steroids, tocilizumab, favipiravir, and ivermectin. However, the immunosuppressive effects of these medicines might worsen COVID-19 symptoms and put the lives of immunocompromised individuals in peril. Thus, it is important to sustain a robust immune system when undergoing therapy for COVID-19. Herbal treatment has the potential to accomplish this objective. Objective: The current investigation involves the preparation of polyherbal syrup containing various medicinal plants such as ephedra, diascorea, ginger, echinacea, garlic, rhubarb, and glycyrrhiza for the effective control of the COVID-19 infection. Methods: All varieties of the individual plant powders (200 g) were treated to a 7 day maceration in aqueous ethanol (70:30) in a percolator at room temperature with intermittent vigorous shaking at room temperature and storage of the extract in a dark room. The mixture was run through a muslin cloth and then a Whatman qualitative grade 1 filter paper to produce the filtrate. The filtrate was evaporated to a thick paste-like consistency at 370 °C under decreased pressure in a rota evaporator connected to a vacuum pump. After that, each individual extract was collected and kept in airtight jars at 4°C. According to the Indian Pharmacopoeia, simple syrup (66.67% w/v) of polyherbal extract was prepared. The oral administration of polyherbal syrup was carried out at varied doses of 0.5 ml, 1 ml, and 1.5 ml to infected golden Syrian hamsters from the 7th day for one week after infection reached its peak. Results: When compared to the infection control group, the results revealed that the viral load was significantly reduced by 79.1% when treated with polyherbal syrup. A histological examination of the infected hamster lung on days 7, 10, and 13 demonstrated that polyherbal syrup significantly decreased viral load in a dose-dependent manner. Conclusion: It is inferred that the polyherbal syrup formulation demonstrates efficacy in the prevention of COVID-19 infection during its first stages and may serve as a potential contender for SARSCoV- 2 due to its immunomodulatory properties.

Safety and efficacy of a polyherbal formulation from traditional Persian medicine in patients with calcium kidney stones: A randomized, double-blinded clinical trial.

10%-15% of the world's population suffers from kidney stones. Nearly 50% increase was observed in diagnosing and treating nephrolithiasis in the last decades. Effective medical treatment for the disease is not yet well established. Moreover, there is an increasing global demand to manage diseases using complementary and alternative medicine. This study aimed to formulate and assess the safety and efficacy of a multi-ingredient formulation from traditional Persian medicine (TPM) known as Mofatet powder in patients suffering from calcium kidney stones. The aqueous extract of Mofatet powder was prepared, freeze-dried, and formulated as capsules. 26 patients in the drug group and 25 patients in the placebo group used 500 mg capsules of the drug/placebo twice daily for 5 weeks. Ultrasonography/kidney, ureter and bladder imaging, urine analysis, and biochemical parameters were evaluated before and after the intervention. The imaging results showed a 60.73% decrease (P < 0.001) in stone size in the drug group. Moreover, the urinary calcium decreased (P = 0.02) and the urinary magnesium increased (P < 0.001) in the drug group. No remarkable changes were observed in the placebo group in these parameters. No significant effect was observed in aspartate transaminase, alanine transaminase, serum creatinine, and blood urea nitrogen levels in none of the groups. This study suggests that Mofatet powder was effective in reducing calcium kidney stones size with no potential nephro/hepatotoxicity. After confirming these results in larger clinical trials with longer duration, this formulation can be considered a treatment for nephrolithiasis.

Deciphering the molecular mechanisms underlying anti-pathogenic potential of a polyherbal formulation Enteropan® against multidrug-resistant Pseudomonas aeruginosa.

Anti-pathogenic potential of a polyherbal formulation Enteropan® was investigated against a multidrug-resistant strain of the bacterium Pseudomonas aeruginosa. Growth, pigment production, antibiotic susceptibility, etc., were assessed through appropriate in vitro assays. Virulence of the test pathogen was assessed employing the nematode worm Caenorhabditis elegans as a model host. Molecular mechanisms underlining the anti-pathogenic activity of the test formulation were elucidated through whole transcriptome analysis of the extract-exposed bacterial culture. Enteropan-pre-exposed P. aeruginosa displayed reduced (~70%↓) virulence towards the model host C. elegans. Enteropan affected various traits like biofilm formation, protein synthesis and secretion, quorum-modulated pigment production, antibiotic susceptibility, nitrogen metabolism, etc., in this pathogen. P. aeruginosa could not develop complete resistance to the virulence-attenuating activity of Enteropan even after repeated exposure to this polyherbal formulation. Whole transcriptome analysis showed 17% of P. aeruginosa genome to get differentially expressed under influence of Enteropan. Major mechanisms through which Enteropan exerted its anti-virulence activity were found to be generation of nitrosative stress, oxidative stress, envelop stress, quorum modulation, disturbance of protein homeostasis and metal homeostasis. Network analysis of the differently expressed genes resulted in identification of 10 proteins with high network centrality as potential targets from among the downregulated genes. Differential expression of genes coding for five (rpoA, tig, rpsB, rpsL, and rpsJ) of these targets was validated through real-time polymerase chain reaction too, and they can further be pursued as potential targets by various drug discovery programmes.

Safety and Hepatoprotective Assessments of Polyherbal Formulation in Lead and Acetaminophen-induced Hepatotoxicity in Rats

Liver disease is a major health problem as it continues to lead to so many deaths worldwide. The cost of treatment for liver diseases is very costly, thus treatment options become available to only a select few.

Standardization of Kandankathiriyaadhy Kyazham: A Classical Siddha Drug

Kandankathiriyaadhy Kyazham is one of the polyherbal formulation mentioned in siddha system of medicine. The main objective of the study was to evaluate the quality of formulation Kandankathiriyaadhy Kyazham by conducting Physicochemical, Phytochemical evaluation through advanced analytical techniques. The organoleptic nature of the drug provides the purity and quality of the formulation. The results obtained from physicochemical evaluation shows that the total ash value of Kandankathiriyaadhy Kyazham was “2.8±0.3%”, in which the acid insoluble ash was “0.041±0.008%”, loss on drying at 105ºC of the formulation Kandankathiriyaadhy Kyazham was noted to be “7.4±0.88” in which water soluble extract value and alcohol soluble extract value was “20.2±1.53 and 8.333=1.15% “respectively. High performance Thin Layer Chromatography analysis of reveals the appearance Kandankathiriyaadhy Kyazham of ten prominent peaks corresponds to presence of ten versatile phyto components present with in it.Rf value of the peaks ranges from “0.13 to 0.91”. Phyto chemical analysis of Kandankathiriyaadhy Kyazham reveals the emergence of phytocomponents like Alkaloids, Flavonoids, Steroids, Triterpenoids, Coumarin, Phenol, Tannin, Protein, Saponins, Sugar, Betacyanin. In conclusion, the polyherbal formulation of Kandankathiriyaadhy Kyazham possess significant phytocomponents and have beneficial effects towards treating various disorders.

Standardization Quality Control and Development of Poly Herbal Formulation for the Management of Type-2 Diabetes Mellitus

In the Ayurvedic system of medicine, Herbal Drugs For Diabetes Mellitus as mentioned in ancient Indian books like Charak Samhita, Mahdhav Nidan and Astang Sanghra, there are about 600 plants, which are stated to have antidiabetic property Polyherbal antidiabetic formulation consists of six herbs viz., Nigella sativa (seed), Moringa oleifera (seed), Linum usitatissimum (seed), Trogonella foenum (seed), Cinnamum zeylanicum (bark) and Macrotylom auniflorum (seed). Crospovidone, Microcrystalline cellulose, Colloidal Silicon dioxide, PVP Magnesium stearate, (Polyvinyl pyrrolidone). Polyherbal formulation of the extracts of all selected plants was subjected to freeze drying process. The extracts were dried for a period of time according to their rate of drying .Diluents like, Microcrystalline cellulose, Magnesium stearate, Lactose, starch were dried. All active ingredients were weighed according to the formula, mixed with MCC followed by diluents and Glidant like aerosil and magnesium stearate as lubricant as specified in formula were mixed well. The mixture was blended thoroughly for 30minutes. Then the powder was transferred to the polythene bags and labeled for further studies.

Ethnobotany of Washim District (M.S.) India

Present paper documents 106 plant species belonging to 44 families used by rural and tribal people of Washim district. Eighty one species are used to treat human ailments – 56 species being used in monoherbal treatments while, 31 species are used in polyherbal formulations. Ten specis are used as veterinary medicine. Eighteen species are edible; while six are used for miscellaneous purposes.

Comparative Study of Phytochemicals present in a Polyherbal Anti-diabetic Formulation using Chemical methods and HPTLC methods

The important challenge to Herbal products for their worldwide acceptance is developing the standard methods for their quality assurance. Qualitative and quantitative analysis of the phytochemicals present in any herbal product plays very important role in its further standardisation. BSL-150 is an Indian polyherbal, anti-diabetic tablet formulation which majorly contains leaves of Gymnemasylvestre, seeds of Syzygiumcumini, seeds of Phyllanthusemblica and bark of Tinospora cordifolia. The phytochemical analysis of the marketed tablets and in-house formulation by standard chemical methods showed presence of Alkaloids, Flavanoids, Steroids, Triterpenoids, Saponins, Tannins, Phenols, Phytosterols, Proteins, Anthraquinones, Gums and mucilages. The phytochemical analysis using HPTLC methods showed presence of Flavanoids, Steroids, Triterpenoids, Saponins, Tannins, Phenols, sterols, Anthraquinones, Essential oils, Bitter principles and coumarins. On comparison of the chemical method and HPTLC method for phytochemical screening, the chemical methods are less time consuming but they are not plant specific and general. Whereas, HPTLC methods can be plant specific and can be developed further for polyherbal formulation. Also, on the basis of intensity and Rf of a band, the selection of the phytochemicals as chemical marker for standardisation of the polyherbal product becomes easy. Thus Modern instrumental method of phytochemical screening has more advantages over the chemical method.

Subacute Oral Toxicity Studies of Pankajakasthuri Orthoherb Tablets Formulated for Managing Pain and Inflammation of Joints

Objectives The objective of this investigation was to assess the subacute oral toxicity of the Pankajakasthuri Orthoherb Tablets, a polyherbal formulation used to manage pain and inflammation due to various pathological reasons, including osteoarthrosis, polyarthritis, rheumatoid arthritis, and other chronic degenerative disorders. Materials and Methods The subacute toxicity studies of Pankajakasthuri Orthoherb Tablets were conducted using Wistar albino rats of both sexes, according to Organization for Economic Cooperation and Development-approved procedures. In this study, rats were orally administered Pankajakasthuri Orthoherb Tablets daily at doses of 250, 500, and 1000 mg/kg body weight for 28 days. Throughout the study period, general behavior, mortality, and adverse effects were recorded. Body weight, selected hematological and clinical chemistry parameters were determined at the end of the investigation period. Finally, histopathological analysis was performed to determine whether the study drug was inducing any alternations in the vital organs. Results Daily administration of Pankajakasthuri Orthoherb Tablets for a continuous 28 days did not record any major toxicity indications. Additionally, no significant variation in food and water consumption, body weight, relative organs and hematology and clinical chemistry parameters was recorded in both sexes compared to the control. Histopathological investigation of the vital organs also revealed normal architecture, which suggests no morphological alterations after the administration of Pankajakasthuri Orthoherb Tablets. Conclusion The outcomes of this investigation confirmed that Pankajakasthuri Orthoherb Tablets did not have the potential to induce toxicity signs in the experimental rats. The No observed Adverse Effect Level (NOAEL) for female and male rats of Pankajakasthuri Orthoherb Tablets under these experimental conditions was 500 mg/kg body weight. Based on the NOAEL, the human equivalent dose of the Pankajakasthuri Orthoherb Tablet was calculated at 5.195 g/day.

Simultaneously quantification of eight marker compounds by HPLC, and HPTLC analysis for the marker-based shelf-life kinetic study for the standardization of polyherbal drug AYUSH SG-5, medicinal properties and computational studies

The herbal or alternative medicines having their own significances in each country. The major problem behind the non-acceptance of the alternative medicines is the poor standardization and quality control. Maximum times, it’s impossible to quantify the complex matrix of polyherbal medicines. The pharmacopeial standards of these medicines are limited to physicochemical and pharmacognostic analysis only, which are insufficient for the proper quality control of ingredients used for the preparation of polyherbal formulations. Multi-markers-based analysis of alternative medicines and their polyherbal formulations by using the sophisticated analytical techniques like HPLC, and LC-MS is the demand of current scenario. In our recent report we have reported pharmaceutical standard operating procedure for a coded polyherbal formulation (CPF i.e. AYUSH-SG-5) which was prepared for the rheumatoid arthritis. The marker-based quantification and identification of major metabolites and shelf-life (marker-based) studies were not reported in that study. In this report we have quantified eight markers (6-Gingerol, 8-Gingerol, Guggulsterone-E, Guggulsterone-Z, Resveratrol, Salicin, Colchicine, and Caffeic acid) simultaneously by HPLC chromatography. To identify the phytochemicals in polyherbal formula (CPF), LC-QTOF-MS/MS analysis was performed, where 32 main phytochemicals were identified out of 80 peaks. In herbal industry, HPTLC is the main tool for drug quantification, so we have reported a HPTLC method for the real time shelf-life study. In HPTLC study, we have used three major markers (6-Gingerol, Guggulsterone-E, Guggulsterone-Z) that are reproducible in hydroalcoholic extract and not in aqueous and methanolic extracts of CPF. The kinetic study-based shelf-life of CPF formulation was performed, the observed shelf-life of CPF was more than 41 months. In addition, evaluation of antioxidant activity like total phenolic (TPC), total flavonoid (TFC) and DPPH assays were performed, and results exhibited that hydroalcoholic extract having more values than aqueous and methanolic extracts of CPF. The phytochemicals observed in LC-QTOF-MS/MS studies were tested for in silico anti-inflammatory activity and anti-arthritic activities, which need to be explored after the detailed pharmacological and biological studies. Overall, analytical studies result of present study could use to standardize the CPF as well as the all relevant polyherbal formulations where these raw drugs have been used.

Evaluation of In vitro Antioxidant and Antidiabetic activity of a Polyherbal formulation

Recent years have seen significant improvements in the identification, management, and study of metabolic illnesses, particularly diabetes. In addition to the present synthetic medicines, current research is focused on developing an alternate source of medication from natural resources for managing diabetes. Objective: To investigate the antioxidant and antidiabetic efficacy of a poly-herbal formulation (PHF) by in vitro models. Methods: The Polyherbal formulation was screened for the presence of phytochemical constituents. The total phenolic and flavonoids content of the formulation was also analysed. The in vitro antioxidant and antidiabetic activity of the formulation (PHF) were calculated. Results: The Polyherbal formulation showed presence of phenols, flavonoids, steroids, and saponins. The IC50 value of PHF4 was found to be 42.70µg/ml in DPPH radical scavenging assay. It has shown high levels of phenols (172.6±0.68 mg/g), flavonoids (137.3±0.91 mg/g) and had IC50 values of 422.50 µg/ml and 438.71 µg/ml for amylase and glucosidase, respectively. Conclusion: The results indicate that Polyherbal Formulation (PHF4) had significant antioxidant activity as well as a strong inhibitory effect on α -amylase and α -glucosidase. However, for further validation of the above findings, in vivo antidiabetic study of polyherbal formulation will be carried out using animal models.

Qualitative and quantitative analysis of siddha herbal Formulation Kabasura kudineer in various concentrations

Introduction: Siddha system is one of the ancient systems coming practised in southern part of India, North and Eastern parts of Sri Lanka. The Siddha medicines are categorised as Aga marundhugal (Internal Medicine) and Pura marundhugal (External Medicine).One among them is kudineer (Decoction). Kabasura kudineer chooranam (KSK) is a polyherbal formulation and it contains 15 ingredients. It is indicated for Iyyasuram and it used for fever with Respiratory disease. Objective and Methods: To analyse the presents of different phyto constituents, develop HPTLC fingerfrint and quantify the amount of phenols and alkaloids in Kabasura Kudineer prepared at 1/4 (sample A), 1/8 (Sample B) and 1/16 (Sample C) reduction concentrations. Result: Phytochemical analysis of Kabasura Kudineer revealed the presence of alkaloid, phenol, flavonoid and tannin in Sample A, B and C. The Rf value obtained by TLC and HPTLC fingerprint for Sample A are 0.02, 0.04, 0.79, Sample B is 0.04, 0.80 and Sample C is 0.04, 0.43, 0.051 and 0.71. The amount of Alkaloid present in Sample A, B, C 1.61 ,1.03,1.44 and phenol present in Sample A, B, C 17.62 ,19.71,18.60 Conclusion: The Analytical parameters along with TLC photo documentation, HPTLC fingerprinting profile and UV Spectrophometer in various concentration of Kabasura Kudineeer are different in different reduction concentration ratio. Further specific chemical markers can be quantified using HPTLC and the therapeutic efficacy can be explored in further studies.

Investigation of the effects of polyherbal formulations against the combination of a high-fat cafeteria diet along with exercise modalities

The aim of the present study was to make a polyherbal formulation of three herbs that would offset the negative effects of the high-fat cafeteria diet on rats and the beneficial effects of exercise. The ingredients for this herbal remedy included a water extract of Garcinia cambogia fruit, Trigonella foenum-graecum seed, and Glycyrrhiza glabra root in a 1:1:1 ratio. Nine groups of six male Wistar albino rats were chosen. Animals in Group I were normal and fed a standard diet; animals in Group II were given HFCD to prevent disease; animals in Group III were given HFCD plus exercise; while animals in Groups IV and V were given PHF at doses of 400 and 200 mg/kg p. o. Daily, respectively, in addition to HFCD; while animals in Groups VI and VII were exercised obese rats prevented from gaining more weight had their serum glucose, total cholesterol, triglycerides, low-density lipoprotein cholesterol, and very low-density lipoprotein cholesterol levels lowered. HDL cholesterol was raised. Further, the liver biopsy showed increased mononuclear cells; fat globules numbered less; and the enzymes and cells that are regenerating were at lower levels. The polyherbal formulation plus exercise exhibited significant anti-obesity efficacy at 400 mg/kg p. o.; the effect was equivalent to orlistat plus exercise. The research employed HFCD-induced obese rats as a model to investigate the joint effects of PHF and exercise against obesity.

Fermented Polyherbal Formulation Restored Ricinoleic Acid-Induced Diarrhea in Sprague Dawley Rats and Exhibited In Vitro Antibacterial Effect on Multiple Antibiotic-Resistant Gastrointestinal Pathogens.

The involvement of multiple antibiotic-resistant gastrointestinal pathogens in diarrhea aggravates the disease condition uncontrollably. The current study aimed to find and develop a suitable formulation utilizing multiple natural components from known plant sources to augment the current therapeutic outcomes. The hydroethanolic extraction method was applied through boiling and fermentation on ancient observation-based efficacious plant parts for developing the antidiarrheal polyherbal formulation AP-01. An animal study model of diarrhea was used to evaluate the safety and efficacy of the formulation. The formulation was tested in vitro on four different multiple antibiotic-resistant gastrointestinal pathogens collected from the national repository. The formulation depicted no cytotoxicity on normal gut cells and was efficacious at 10 ml/kg single dose in relieving symptoms of diarrhea by 79.71%, compared with the standard drug showing a reduction of symptoms by 83.01%. AP-01 exhibited delayed gastric motility. The symptoms of diarrhea ceased to occur within 320.66 ± 5.05 minutes with AP-01, whereas the standard drug took 308 ± 6.63 minutes. AP-01 was found successful at a viable dosage regimen of 75 μl/ml v/v to 100 μl/ml v/v in inhibiting the growth of different pathogens from the Enterobacteriaceae family possessing resistance against several classes of antibiotics in culture media. Chemical analysis revealed different alkaloids, flavonoids, and polyphenols that probably work in unison through multiple modes of action to arrest diarrhea and inhibit pathogens simultaneously. These promising results shown by AP-01 should evoke an effort to dive deep into research and development for better therapeutic formulations for infectious diarrhea by harvesting nature's arsenal.

In silico network pharmacology study on Glycyrrhiza glabra: Analyzing the immune-boosting phytochemical properties of Siddha medicinal plant against COVID-19.

Immunosenescence is a pertinent factor in the mortality rate caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The changes in the immune system are strongly associated with age and provoke the deterioration of the individual's health. Traditional medical practices in ancient India effectively deal with COVID-19 by boosting natural immunity through medicinal plants. The anti-inflammatory and antiviral properties of Glycyrrhiza glabra are potent in fighting against COVID-19 and promote immunity boost against the severity of the infection. Athimadhura Chooranam, a polyherbal formulation containing Glycyrrhiza glabra as the main ingredient, is recommended as an antiviral Siddha herb by the Ministry of AYUSH. This paper is intended to identify the phytoconstituents of Glycyrrhiza glabra that are actively involved in preventing individuals from COVID-19 transmission. The modulated pathways, enrichment study, and drug-likeness are calculated from the target proteins of the phytoconstituents at the pharmacological activity (Pa) of more than 0.7. Liquiritigenin and Isoliquiritin, the natural compounds in Glycyrrhiza glabra, belong to the flavonoid class and exhibit ameliorative effects against COVID-19. The latter compound displays a higher protein interaction to a maximum of six, out of which HMOX1, PLAU, and PGR are top-hub genes. ADMET screening further confirms the significance of the abovementioned components containing better drug-likeness. The molecular docking and molecular dynamics method identified liquiritigenin as a possible lead molecule capable of inhibiting the activity of the major protease protein of SARS-CoV-2. The findings emphasize the importance of in silico network pharmacological assessments in delivering cost-effective, time-bound clinical drugs.

Pharmacognostic standardization and HPTLC fingerprinting analysis of Sahacharadi Kashayam: a classical ayurvedic polyherbal formulation

Multiherbal compositions are widely practiced in ayurvedic medical system, and ka??yam (decoction) is one such dose. Sahacharadi Kashayam is a traditional ayurvedic polyherbal medicine recommended by ayurvedic doctors for chronic and acute back pains, lower limb disorders, and conditions that mainly affect the nutrient level in the blood and the bone. The aim of this study was to develop pharmacognostic parameters for the standardization and quality evaluation of the Sahacharadi Kashayam. In-house polyherbal formulation was prepared as per the previously prescribed method, and physicochemical parameters were evaluated as per standard protocol and showed loss on drying at 99.31%, total ash content at 4.911 ± 0.120%, water-soluble ash at 3.022, and acid-insoluble ash at 0.489. Total phenolic content was estimated with FC reagent and expressed as gallic acid equivalent (9.95 + 0.004 mg/g). High-performance thin-layer chromatography (HPTLC) fingerprint profiling was obtained, and the quantity of total tannins was estimated as tannic acid equivalent using HPLC (0.47%). The results obtained from the study could be utilized as a reference for the quality control and quality assurance of Sahacharadi Kashayam.

Evaluation of In Vitro Acid Neutralizing Capacity of Bilwadi Vati

Herbs have been used for thousands of years in traditional medicine systems, such as Ayurveda, to treat various ailments, including hyperacidity. The aim of this study was to investigate the acid neutralizing properties of Bilwadi Vati, a polyherbal formulation used to treat the intestinal disorders. A total of 14 herbal ingredients of Bilwadi Vati were tested using an in vitro assay to determine their Preliminary antacid test andacid neutralizing capacity. The results showed that Bilwadi Vati as a whole and all the herbal ingredients tested exhibitacid neutralizing properties to varying degrees. Among the tested herbal ingredients Sunthi (Zingiber officinale), Maricha (Piper nigrum), Nata (Valerianawalichi), Haridra (Curcuma longum) and Daruharidra (Berberis aristata) five ingredients demonstrated the significantly higher acid neutralizing capacity. This study supports and sheds light on the purported digestive and antiacidic properties of Bilwadi Vati and its individual ingredients. Further study to determine the in vitro neutralizing and buffering capacity of ingredients is in process.

Antidiabetic herbal drugs and polyherbal formulation used for diabetes: A review

Antidiabetic herbal drugs and polyherbal formulation used for diabetes: A review

Anti-inflammatory and pro-apoptotic properties of the polyherbal drug, MAT20, in MCF-7 cells

Breast cancer remains a serious health issue with approximately 2.3 million women diagnosed with breast cancer in 2020. Standard treatment approaches include surgery, chemotherapy, and radiation. However, these treatments have side effects such as drug resistance, metastasis, and relapse. Therefore, it is necessary to investigate alternative therapy approaches that may mitigate the adverse effects of standard therapies. The emerging field of complementary and alternative medicine (CAM) presents an intriguing possibility of using natural products as therapeutics. The main objective of this study was assessing the anticancer efficacy of MAT20, a polyherbal formulation with extracts of moringa, amla, and tulsi. In this study, we examined the anti-inflammatory properties of MAT20, along with phorbol 12-myristate 13-acetate (PMA) and 5-fluorouracil (5-FU), on the MCF-7 breast cancer cell line. Specifically, we assessed the expression of the COX2 gene, a key marker of inflammation. We hypothesized that the addition of MAT20, targeting COX2 downregulation, may have a dual impact by not only reducing inflammation but also regulating programmed cell death pathways by apoptotic gene expression studies. Our results demonstrated that MAT20 decreased the PMA-induced COX2 expression in MCF-7 cells. MAT20 treatment in MCF-7 cells showed upregulation of BAX and BAD (pro-apoptotic genes) and downregulation of BCL2 and BCL2L1 (anti-apoptotic genes) in MCF-7 cells. Overall, our results showed that MAT20 may have anti-inflammatory and pro-apoptotic effects on MCF-7 cells.

Determination of phytochemical markers andrographolide, eugenol and zingerone in nilavembu kudineer by RP-HPLC method

In the present research, the reverse phase (RP)-high performance liquid chromatography (HPLC) method was developed and validated to determine phytochemical markers andrographolide, eugenol, and zingerone in nilaveembu kudineer (NK) a Siddha polyherbal formulation. There are no RP-HPLC methods reported for the simultaneous estimation of these marker compounds in any herbal formulation. In this study, the optimized chromatographic conditions were C18 Column (150 × 4.6 × 5 μm) mobile phase containing Acetonitrile and 0.1% Ortho Phosphoric acid 30:70, flow rate 1.0 ml/minutes, at 254 nm using a ultravoilet detector, column temperature of 40°C and run time of 20 minutes. In the linearity study, a linear relation was observed from 25 to 200 μg/ml with a correlation coefficient of 0.9990. The optimized method is successfully applied for the estimation of these phytochemical markers in NK formulation. The suggested approach is an excellent quality control tool for the simultaneous quantitative assessment and detection of phytochemical markers present in polyherbal formulations and in traditional medicines such as ayurveda, siddha, unani, and homeopathic formulations.