BackgroundThis study investigates the effects of Ginkgo Diterpene Lactone Meglumine (GM) combined with Clopidogrel (CLO) on hemodynamics, neurocytokines, and inflammatory responses in patients with cerebral infarction (CI) complicated by coronary heart disease (CHD).MethodsA total of 152 patients diagnosed with CI complicated by CHD, admitted to our hospital between January 2024 and October 2024, were enrolled in the study. Among them, 81 patients received CLO monotherapy (control group), while the remaining 71 patients were treated with a combination of CLO and GM (observation group). Hemodynamic parameters, including plasma viscosity (PV), whole blood high (WBHSV) and low shear viscosity (WBLSV), as well as reduced viscosity (RV), were measured before and after treatment. Platelet adhesion test (PAdT) and platelet aggregation test (PAgT) were also performed. Inflammatory markers and neurocytokines were assessed using enzyme-linked immunosorbent assays, and adverse reactions during treatment were documented.ResultsAfter treatment, both groups exhibited significant reductions in PAdT, PAgT, PV, WBHSV, WBLSV, and RV compared to baseline (P<0.05). However, PAdT, PAgT, WBHSV, WBLSV and RV were lower in the observation group compared to the control group (P<0.05). Additionally, the observation group showed lower levels of neuron-specific enolase, glial fibrillary acidic protein, tumor necrosis factor-a, and hypersensitive C-reactive protein, along with higher levels of brain-derived neurotrophic factor, compared to the control group (P<0.05). No significant difference was observed in the incidence of adverse reactions between the two groups (P>0.05).ConclusionsThe combination of GM and CLO is more effective than CLO monotherapy in improving hemodynamics, enhancing neurological function, and mitigating inflammatory responses in patients with CI complicated by CHD.

A syndiotactic poly(substituted methylene) (st-PM) featuring (2-methoxyethoxy)carbonyl side chains (st-PMECM) exhibited considerably superior antithrombogenicity compared to a typical conventional antithrombogenic polyacrylate comprising the same side chain (i.e., poly(2-methoxyethyl acrylate); PMEA). The number of adherent platelets on the surface of the st-PMECM films was 0.8 × 103 mm-2, which was one-fourth of that on the PMEA films (3.2 × 103 mm-2), and 75% of these platelets were in the lowest activation state. During the platelet adhesion test, the st-PMECM films maintained surface wettability on the substrates, addressing the commonly observed dewetting issue in the practical applications of conventional antithrombogenic polyacrylates. In addition, the st-PMECM films retained their surface structure upon exposure to plasma, as indicated by the unchanged water contact angle over time, unlike in the case of polyacrylates. These characteristics of st-PMECM are attributed to the formation of liquid crystal structure, with the backbone arranged into a two-dimensional lattice and with the side chains stretched at an angle to the backbone axis. Owing to this structure, the methoxy moieties at the ends of the side chains were exposed to the outermost surface and hydrated with water, enabling the st-PMECM films to possess a larger amount of intermediate water on the surface than the PMEA films. The results demonstrate that st-PMs more effectively enhance the functionality of the side chains than polyacrylates featuring the same side chains, enabling st-PMECM to exhibit superior antithrombogenicity attributed to methoxy moieties. To the best of our knowledge, st-PMECM is the first inherently solid-like polymer that simultaneously exhibits outstanding antithrombogenicity.

Magnesium alloy represents a typical category of biodegradable medical materials. However, the poor corrosion resistance and rapid degradation have significantly hindered the clinical adoption of magnesium alloy implants. This paper puts forward a method to improve the corrosion resistance of magnesium alloy by using an Fe-based composite coating. The microstructure and composition of the coating were analyzed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive spectroscopy (EDS). The corrosion resistance was evaluated through potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) measurements conducted in simulated body fluid, while the degradation behavior of the samples was evaluated by examining the hydrogen evolution volume and corrosion morphology during immersion tests. The results indicate that the composite coating exhibits a dual-layer structure, consisting of an amorphous carbon–fluorine transition layer and an iron-rich surface layer. After coating treatment, the corrosion current density of magnesium alloy decreased from 1.38 × 10−4 to 3.41 × 10−6 A/cm2. Throughout a 28-day immersion period, the composite-coated sample demonstrated a remarkably low hydrogen evolution rate and maintained a smooth, intact surface. Furthermore, hemolysis and platelet adhesion tests confirmed the outstanding blood compatibility of the composite-coated magnesium alloy, showing an ultralow hemolysis rate of 0.1% and minimal platelet adhesion with well-preserved morphology.

ABSTRACT The membrane oxygenator is very important in extracorporeal membrane oxygenation (ECMO) systems, which are equivalent to the lung to achieve the gas exchange of O 2 and CO 2 for the blood. The membrane oxygenator should be highly hemocompatible and a good barrier between the gas and blood. Herein, novel composite membranes with ultra‐thin defect‐free dense layers were constructed successfully on the porous polyacrylic fibre (PAN) substrate by the fluororubber materials for the ECMO system. The O 2 and CO 2 permeance of the defect‐free PAN/fluororubber composite membranes was very high (J O2 = 2054 GPU, J CO2 = 3491 GPU), which was very beneficial for the exchange of breathing gases and much higher than the commercial PMP membranes (J O2 = 131 GPU, J CO2 = 376 GPU). Simultaneously, the PAN/fluororubber composite membranes exhibited good biocompatibility through conducting biocompatibility experiments (protein adsorption evaluation, coagulation tests and platelet adhesion test). Above all, the novel composite membranes are very promising for application in ECMO oxygenators.

Thromboembolic complications still arise on blood contacting surfaces. Surface charge and topography influence the subsequent deposition of proteins and platelets, potentially leading to thrombi. Research showed a correlation of surface charge and nanoscale roughness, and a negative surface charge as well as a smooth surface finish are associated with lower thrombogenicity. The aim of this study was to compare the platelet adhesion on titanium with different nanoscale roughnesses and to examine if those roughness variations caused a change in surface charge. Titanium samples were polished and roughened to four different nanoscale roughness levels. Platelet adhesion (covered surface area (CSA), N = 8) was tested in flow chambers with human whole blood using fluorescence imaging. ζ-potential was measured over a broad range of pH-values and interpolated to obtain the ζ-potential for pHBlood (7.4). Platelet adhesion tests were evaluated in terms of p-values and the Wilcoxon test effect size and the trend of the ζ-potential at pHBlood and the CSA was compared. Ra-values ranged between 35 (polished) and 156 nm. Regarding platelet adhesion, the polished sample showed the lowest mean CSA with a medium or strong effect size compared to the roughened samples. The interpolated ζ-potentials for pHBlood follow a similar trend as the CSA, with the lowest ζ-potential measured for the polished surface. These findings suggest that the decreasing ζ-potential due to lower nanoscale roughness might be an additional explanation for the improved hemocompatibility besides the smoother topography.

In this work, we have demonstrated agar and oxidized bacterial cellulose cryogels as a potential hemostatic dressing material. TEMPO-oxidized bacterial cellulose (OBC) was incorporated into the agar matrix, improving its mechanical and hemostatic properties. The oxidation of bacterial cellulose (BC) was evidenced by chemical characterization studies, confirming the presence of carboxyl groups. The in vitro blood clotting test conducted on agar/OBC composite cryogels demonstrated complete blood clotting within 90 seconds, indicating their excellent hemostatic efficacy. The cryogels exhibited superabsorbent properties with a swelling degree of 4200%, enabling them to absorb large amounts of blood. Moreover, the compressive strength of the composite cryogels was appreciably improved compared to pure agar, resulting in a more stable physical structure. The platelet adhesion test proved the significant ability of the composite cryogels to adhere to and aggregate platelets. Hemocompatibility and cytocompatibility tests have verified the safety of these cryogels for hemostatic applications. Finally, the material exhibited remarkable in vivo hemostatic performance, achieving clotting times of 64 seconds and 35 seconds when tested in the rat tail amputation model and the liver puncture model, respectively. The experiment results were compared with those of commercial hemostat, Axiostat, and Surgispon, affirming the potential of agar/OBC composite cryogel as a hemostatic dressing material.

Blood-contacting medical devices such as biodegradable metallic bone implant materials are expected to show excellent hemocompatibility both in vitro and in vivo. Different approaches are being studied and used to modify biomaterial surfaces for enhanced biocompatibility and hemocompatibility. However, the composition of degradable biomaterial must address several drawbacks at once. Iron-reinforced zinc material was used as a metallic substrate with improved mechanical properties when compared with those of pure zinc. Poly(lactic) acid (PLA) or polyethylenimine (PEI) was selected as a polymeric matrix for further doping with antibiotic ciprofloxacin (CPR) and marine-sourced polysaccharide fucoidan (FU), which are known for their antibacterial and potential anticoagulant properties, respectively. Radiofrequency air plasma was employed to induce metallic/polymer-coated surface activation before further modification with FU/CPR. Sample surface morphology and composition were studied and evaluated (contact angle measurements, AFM, SEM, and FT-IR) along with the hemolysis ratio and platelet adhesion test. Successful doping of the polymer layer by FU/CRP was confirmed. While PEI induced severe hemolysis over 12%, the PLA-coated samples exhibited even lower hemolysis (∼2%) than uncoated samples while the uncoated samples showed the lowest platelet adhesion. Moreover, gradual antibiotic release from PLA determined by the electrochemical methods using screen-printed carbon electrodes was observed after 24, 48, and 72 h, making the PLA-coated zinc-based material an attractive candidate for biodegradable material design.

The development of innovative vascular substitutes has become increasingly significant due to the prevalence of vascular diseases. In this study, we designed a biofunctionalized electrospun vascular scaffold by chemically conjugating heparin molecules as an antithrombotic agent with an endothelial cell (EC)-specific antibody to promote in situ endothelialization. To optimize this biofunctionalized electrospun vascular scaffolding system, we examined various parameters, including material compositions, cross-linker concentrations, and cross-linking and conjugation processes. The findings revealed that a higher degree of heparin conjugation onto the vascular scaffold resulted in improved antithrombotic properties, as confirmed by the platelet adhesion test. Additionally, the flow chamber study demonstrated that the EC-specific antibody immobilization enhanced the scaffold's EC-capturing capability compared to a nonconjugated vascular scaffold. The optimized biofunctionalized vascular scaffolds also displayed exceptional mechanical properties, such as suture retention strength and tensile properties. Our research demonstrated that the biofunctionalized vascular scaffolds and the directed immobilization of bioactive molecules could provide the necessary elements for successful acellular vascular tissue engineering applications.

In-stent restenosis and thrombosis remain to be long-term challenges in coronary stenting procedures. The objective of this study was to evaluate the in vitro biological responses of trimethylsilane (TMS) plasma nanocoatings modified with NH3 /O2 (2:1 molar ratio) plasma post-treatment (TMS + NH3 /O2 nanocoatings) on cobalt chromium (CoCr) alloy L605 coupons, L605 stents, and 316L stainless steel (SS) stents. Surface properties of the plasma nanocoatings with up to 2-year aging time were characterized by wettability assessment and x-ray photoelectron spectroscopy (XPS). It was found that TMS + NH3 /O2 nanocoatings had a surface composition of 41.21 ± 1.06 at% oxygen, 31.90 ± 1.08 at% silicon, and 24.12 ± 1.7 at% carbon, and very small but essential amount of 2.77 ± 0.18 at% nitrogen. Surface chemical stability of the plasma coatings was noted with persistent O/Si atomic ratio of 1.292-1.413 and N/Si atomic ratio of ~0.087 through 2 years. The in vitro biological responses of plasma nanocoatings were studied by evaluating the cell proliferation and migration of porcine coronary artery endothelial cells (PCAECs) and smooth muscle cells (PCASMCs). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay results revealed that, after 7-day incubation, TMS + NH3 /O2 nanocoatings maintained a similar level of PCAEC proliferation while showing a decrease in the viability of PCASMCs by 73 ± 19% as compared with uncoated L605 surfaces. Cell co-culture of PCAECs and PCASMCs results showed that, the cell ratio of PCAEC/PCASMC on TMS + NH3 /O2 nanocoating surfaces was 1.5-fold higher than that on uncoated L605 surfaces, indicating enhanced selectivity for promoting PCAEC growth. Migration test showed comparable PCAEC migration distance for uncoated L605 and TMS + NH3 /O2 nanocoatings. In contrast, PCASMC migration distance was reduced nearly 8.5-fold on TMS + NH3 /O2 nanocoating surfaces as compared to the uncoated L605 surfaces. Platelet adhesion test using porcine whole blood showed lower adhered platelets distribution (by 70 ± 16%), reduced clotting attachment (by 54 ± 12%), and less platelet activation on TMS + NH3 /O2 nanocoating surfaces as compared with the uncoated L605 controls. It was further found that, under shear stress conditions of simulated blood flow, TMS + NH3 /O2 nanocoating significantly inhibited platelet adhesion compared to the uncoated 316L SS stents and TMS nanocoated 316L SS stents. These results indicate that TMS + NH3 /O2 nanocoatings are very promising in preventing both restenosis and thrombosis for coronary stent applications.

Polysaccharide-based (kappa carrageenan/carboxymethyl chitosan) nanofibrous membrane loaded with antifibrinolytic drug for rapid hemostasis- in vitro and in vivo evaluation

A phosphorylcholine polymer (poly(MPC–co–BMA–co–TSMA), PMBT) was prepared by free radical polymerization and coated on the surface of the polymethylpentene hollow fiber membrane (PMP–HFM). ATR–FTIR and SEM analyses showed that the PMBT polymer containing phosphorylcholine groups was uniformly coated on the surface of the PMP–HFM. Thermogravimetric analysis showed that the PMBT had the best stability when the molar percentage of MPC monomer in the polymer was 35%. The swelling test and static contact angle test indicated that the coating had excellent hydrophilic properties. The fluorescence test results showed that the coating could resist dissolution with 90% (v/v%) ethanol solution and 1% (w/v%) SDS solution. The PMBT coating was shown to be able to decrease platelet adherence to the surface of the hollow fiber membrane, and lower the risk of blood clotting; it had good blood compatibility in tests of whole blood contact and platelet adhesion. These results show that the PMBT polymer may be coated on the surface of the PMP–HFM, and is helpful for improving the blood compatibility of membrane oxygenation.

Suitable biomechanical properties, good biocompatibility, and osteoconductivity of a degradable magnesium (Mg) alloy make it a potential material for orthopedic implants. The main limitation of Mg is its high corrosion rate in the human body. Surface modification is necessary to improve the Mg corrosion resistance. In this work, a polymeric layer of gelatin/nanohydroxyapatite (Gel/nHA) was coated on a ZK60 Mg alloy by dip coating and spin coating to test the corrosion resistance and biocompatibility in vitro and in vivo. The results from the in vitro test revealed that the coated groups reduced the corrosion rate with the corrosion current density by 59 and 81%, from 31.22 to 12.83 μA/cm2 and 5.83 μA/cm2 in the spin coating and dip coating groups, respectively. The dip coating group showed better corrosion resistance than the spin coating group with the lowest released hydrogen content (17.5 mL) and lowest pH value (8.23) and reducing the current density by 45%. In vitro, the relative growth rate was over 75% in all groups tested with MG63, demonstrating that the Mg substrate and coating materials were within the safety range. The dip coating and spin coating groups enhanced the cell proliferation with significantly higher OD values (3.3, 3.0, and 2.5, respectively) and had better antihemolysis and antiplatelet adhesion abilities than the uncoated group. The two coating methods showed no difference in the cellular response, cell migration, hemolysis, and platelet adhesion test. In in vivo tests in rats, the dip coating group also showed a higher corrosion resistance with a lower corrosion rate and mass loss than the spin coating group. In addition, the blood biochemistry and histopathology results indicated that all materials used in this study were biocompatible with living subjects. The present research confirmed that the two methods have no noticeable difference in cell and organ response but the corrosion resistance of dip coating was higher than that of spin coating either in vitro or in vivo.

Problems of rapid degradation and poor biocompatibility (endothelialization and hemocompatibility) limit magnesium (Mg) alloy’s further applications in vascular stents. To solve these problems, a novel composite coating was designed on Mg alloy via a two-step method. First, a Mg alloy sample was immersed in hydrofluoric acid. Then, a poly-l-lactic acid (PLLA) coating was made by ultrasonic atomization spraying with 5 and 10 layers (referred to as PLLA(5)-HF-Mg and PLLA(10)-HF-Mg). Characterizations were analyzed from the microstructure, element distribution, and wettability. The degradation behavior was tested with an electrochemical test and immersion test. Endothelialization was investigated using human umbilical vein endothelial cells (HUVECs). Hemocompatibility was examined with a platelet adhesion test. The results showed that the PLLA coating could not only cover the surface, but also could permeate through and cover the holes on the MgF2 layer, mechanically locked with the substrate. Thus, the composite coating had higher corrosion resistance. The PLLA/MgF2 coating, especially on PLLA(10)-HF-Mg, enhanced HUVECs’ viability and growth. While incubated with platelets, the PLLA/MgF2 coating, especially on PLLA(10)-HF-Mg, had the lowest platelet adhesion number and activity. Taken together, the novel PLLA/MgF2 coating controls Mg alloy’s degradation by spraying different layers of PLLA, resulting in better endothelialization and hemocompatibility, providing a promising candidate for cardiovascular stents.

Gelatin/calcium chloride electrospun nanofibers for rapid hemostasis.

Abstract Surface heparinization is an effective solution to resolve low endothelialization, poor anticoagulation, and hemocompatibility of polyurethane (PU) used as materials of small‐diameter vascular grafts. Here, the effects of polydopamine (PDA) and poly (acrylic acid) (PAA) as crosslinking agents on the surface heparinization were explored. The PU membranes grafted with heparin (Hep) via dopamine (PU/PDA‐Hep) showed better hydrophilicity and stability, compared to heparinized PU membranes via acrylic acid (PU/PAA‐Hep). The results of X‐ray photoelectron spectroscopy demonstrated that heparin was successfully grafted onto the PU surface and the grafting efficiency was high when PDA as a cross‐linking agent. The grafted heparin aggregated and formed nanoparticles, and increased the surface roughness of PU membranes. The heparinized membranes demonstrated good anti‐adhesion of bovine serum albumin and fibrin protein. In addition, no activated platelets or educts on heparinized PU were found by platelet adhesion tests, implying that heparin‐immobilized surfaces had good hemocompatibility. Moreover, the in vitro cytocompatibility assessment showed that the PU/PDA‐Hep significantly improved the proliferation of L929 cells and was superior to PU/AA‐Hep. These results demonstrated that PDA‐assisted surface heparinization was an effective method to improve the anticoagulant and biocompatibility of PU small‐diameter vascular materials and could be extended to other implantable materials.

Thrombogenicity, which is commonly encountered in artificialheartvalves after replacement surgeries, causes valvular failure. Evenlife-long anticoagulant drug use may not be sufficient to preventthrombogenicity. In this study, it was aimed to develop a heart valveconstruct with antithrombogenic properties and suitable mechanicalstrength by combining multiwalled carbon nanotubes within a decellularizedbovine pericardium. In this context, the decellularization processwas performed by using the combination of freeze–thawing andsodium dodecyl sulfate (SDS). Evaluation of decellularization efficiencywas determined by histology (Hematoxylin and Eosin, DAPI and Masson’sTrichrome) and biochemical (DNA, sGAG and collagen) analyses. Afterthe decellularization process of the bovine pericardium, compositepericardial tissues were prepared by incorporating −COOH-modifiedmultiwalled carbon nanotubes (MWCNTs). Characterization of MWCNT incorporationwas performed by ATR-FTIR, TGA, and mechanical analysis, while SEMand AFM were used for morphological evaluations. Thrombogenicity assessmentswere studied by platelet adhesion test, Calcein-AM staining, kineticblood clotting, hemolysis, and cytotoxicity analyses. As a resultof this study, the composite pericardial material revealed improvedmechanical and thermal stability and hemocompatibility in comparisonto decellularized pericardium, without toxicity. Approximately 100%success is achieved in preventing platelet adhesion. In addition,kinetic blood-coagulation analysis demonstrated a low rate and slowcoagulation kinetics, while the hemolysis index was below the permissiblelimit for biomaterials.

Abstract Poly(3,4‐ethylenedioxythiophene) (PEDOT) and its derivatives have demonstrated potential in the development of bioelectrodes because of their superior conductivity. However, developing reliable implanted bioelectrodes requires improvements in biocompatibility and the prevention of nonspecific adhesion. In this study, a six ethylene glycol (EG)‐functionalized EDOTs with three different EG lengths (tri‐EG, tetra‐EG, and hexa‐EG) and two types of end groups, hydroxyl (−OH) and methoxy (−OCH3) is synthesized and systematically investigated. By coating them on gold electrodes using electropolymerization, the surface and electrochemical properties of these functionalized PEDOT‐coated electrodes are investigated. Although PEDOT with −OH groups on the surface is more hydrophilic, those with −OCH3 groups on the surface exhibit higher electrochemical activity and lower impedance. The increase in EG units and −OCH3 groups on the surface effectively reduces the adhesion between the PEDOT and atomic force microscopy tips. PEDOT with longer EG lengths and −OCH3 groups exhibits relatively few adhered platelets, and the results of the analysis of hydrated states through differential scanning calorimetry are consistent with those of the platelet adhesion test. This study suggests that a tetra(EG)‐functionalized PEDOT with −OCH3 groups on the surface is a promising coating for implanted bioelectrode applications.

Native grafts such as internal mammary artery and saphenous vein are the main choice for coronary artery bypass graft. However, due to the limitations associated with their availability and rapid failure caused by hyperplasia, small diameter tissue-engineered vascular grafts (TEVGs) with sufficient post-implantation patency are urgently demanded as artificial alternatives. In our previous work, we innovatively fabricated a bilayer vascular graft providing appropriate structural and biological properties using electrospinning and freeze-drying methods. It was proved that the mechanical properties of the proposed graft enhanced in comparison with using either of methods individually. Here, we adopted the same methods and incorporated an anticoagulant internal layer (inner diameter 4 mm), comprised of co-electrospun fibers of silk fibroin (SF) and heparinized thermoplastic polyurethane (TPU), and an external highly porous hydrogel fabricated by freeze-drying method. The electrospun layer exhibited strong mechanical properties including superior elastic modulus (4.92 ± 0.11 MPa), suture retention force (6.73 ± 0.83 N), elongation at break (196 ± 4%), and comparable burst pressure (1140 ± 12 mmHg) while the external hydrogel provided SMCs viability. The heparin was released in a sustain manner over 40 days, and the cytocompatibility and blood compatibility of scaffold were approved using MTT assay and platelet adhesion test. Thus, the proposed graft has a potential to be used as an artificial blood vessel scaffold for later in-vivo transplantation.

Drug eluting stents (DES) can efficiently reduce the atherosclerosis and restenosis issues of coronary artery as compared to bare metal stents due to the presence of pharmaceutically active agent on their surface. Nevertheless, the arising safety concerns of DES such as delayed healing and late in stent restenosis and thrombus, has stirred the research efforts to improve the outcomes of the DES. In this connection, attention is being shifted from the use of synthetic drug to natural drug for DES. In the present work, natural compound loaded polymeric films were synthesized and their antioxidant and anticoagulation capabilities were assessed through in vitro testing. The potential of the drug loaded polymeric films to curb the production of free radicals was evaluated by carrying out antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The in vitro platelet adhesion was investigated through static platelet adhesion test while effect of synthesized films on intrinsic coagulation pathway was investigated through activated partially thromboplastin time (APTT). Moreover, to further evaluate the blood compatibility of the developed drug loaded films, in vitro hemolytic and anti-thrombolytic assays were carried out. The obtained results indicated that, incorporating herbal compounds such as ginger, magnolol and curcumin, in polymeric matrix (PVA) has significantly improved the blood compatibility of the polymeric films. Hence, it can be concluded that the synthesized drug loaded polymeric films have the potential capability to be used as a potential coating material for coating biomedical implants with good anticoagulation and antioxidant property to cater the cardiovascular issues such as atherosclerosis, restenosis and thrombus formation.

PurposeThe purpose of this paper is to fabricate pre-existing geometries of the stents using solvent cast 3D printing (SC3P) and encapsulation of each stent with heparin drug by using aminolysis reaction.Design/methodology/approachThe iron pentacarbonyl powder and poly-ɛ-caprolactone blend (PCIP) were used to print stent designs of Art18z, Palmaz-Schatz and Abbott Bvs1.1. The properties of antithrombosis, anticoagulation and blood compatibility were introduced in the stents by conjugation of heparin drug via the aminolysis process. The aminolysis process was confirmed by energy dispersive X-ray spectroscopy and Fourier transform infrared spectroscopy due to presence of amide group and nitrogen peak in the respective analysis. Biological studies were performed to depict the cell viability, hemocompatibility and antithrombotic properties. Besides, mechanical behaviors were analyzed to study the behavior of the stents under radial compression load and bending load.FindingsThe amount of heparin immobilized on the Art18z, Palmaz-Schatz and Abbott Bvs1.1 stents were 255 ± 27, 222 ± 30 and 212 ± 13 µg, respectively. The cell viability studies using L929 fibroblast cells confirmed the cytocompatibility of the stents. The heparinized SC3P printed stents displayed excellent thrombo-resistance, anticoagulation properties and hemocompatibility as confirmed by blood coagulation analysis, platelet adhesion test and hemolysis analysis. Besides, mechanical behavior was found in context of the real-life stents. All these assessments confirmed that the developed stents have the potential to be used in the real environment of coronary arteries.Originality/valueVarious customized shaped biodegradable stents were fabricated using 3D printing technique and encapsulated with heparin drug using aminolysis process.