Larsen, S, de Zee, M, Kristiansen, EL, and van den Tillaar, R. A biomechanical comparison between a high and low barbell placement on net joint moments, kinematics, muscle forces, and muscle-specific moments in 3 repetition maximum back squats. J Strength Cond Res 38(7): 1221-1230, 2024-This study aimed to investigate the impact of a high barbell vs. low barbell placement on net joint moments, muscle forces, and muscle-specific moments in the lower extremity joints and muscles during maximum back squats. Twelve recreationally trained men (age = 25.3 ± 2.9 years, height = 1.79 ± 7.7 m, and body mass = 82.8 ± 6.9 kg) volunteered for the study. A marker-based motion capture system and force plate data were used to calculate the net joint moments, and individual muscle forces were estimated using static optimization. Muscle forces were multiplied by their corresponding internal moment arms to determine muscle-specific moments. Statistical parametric mapping was used to analyze the effect of barbell placement as time-series data during the concentric phase. The 3 repetition maximum barbell load lifted by the subjects was 129.1 ± 13.4 kg and 130.2 ± 12.7 kg in the high bar and low bar, which were not significantly different from each other. Moreover, no significant differences were observed in net joint moments, muscle forces, or muscle-specific moments for the hip, knee, or ankle joint between the low- and high bar placements. The findings of this study suggest that barbell placement plays a minor role in lower extremity muscle forces and moment-specific moments when stance width is standardized, and barbell load lifted does not differ between barbell placements among recreationally resistance-trained men during maximal back squats. Therefore, the choice of barbell placement should be based on individual preference and comfort.