Pine Bark Research Articles

English

Tomato production under greenhouse conditions needs vigorous seedlings to reduce the loss of plants after the transplant. Different local substrates such as agave bagasse, pine bark and coconut fiber complemented with peat, expanded clay, vermiculite and pure peat for tomato seedlings production were tested. The seed germination, root ball compaction, height of the seedling, stem diameter, and total dry weight were quantified. The stem diameter observed in Agave - bagasse + peat + expanded - clay + vermiculite (30:60:5:5 v/v) (BMTAEV3), Agave - bagasse + pine - bark + peat + expanded - clay + vermiculite (30:30:30:5:5) (BMCPTAEV4), Agave - bagasse + peat + expanded - clay + vermiculite (50:30:10:10) (BMTAEV5), Sphagnum peat (SUNSHINE® 3) 100% (M3SC), and Agave - bagasse + peat + expanded - clay + vermiculite (40:40:10:10) (BMTAEV6) treatments were in acceptable parameter range to commercial use, but BMTAEV3, BMTAEV5, and BMTAEV6 treatments show better germination, root ball compaction, a bigger height and diameter, getting tomato seedlings with vigor to the transplant. The local substrata overcome the commercial growth medium parameters in seedlings evaluation. It is shown that mixtures of agave-bagasse with expanded clay, and vermiculite are a substrate alternative for tomato seedlings production, reducing the use of expensive and scarce material such as peat by up to between 40 and 60% (v/v). Key words: Agave by-product, parameters, substrate, transplant, tomato seedlings.

Valorisation of non-timber by-products from maritime pine (Pinus pinaster, Ait) for particleboard production

Maritime pine (Pinus pinaster, Ait.) is one of the most important trees in the Iberian Peninsula forest. Its wood is mainly used in the wood-based panels production and for pulp and paper factories. The aim of this work was the valorisation of the main forest by-products of the P. Pinaster: bark, pinecones and needles. These organic by-products were widely used in small rural farms, but nowadays are mostly left in the forest, contributing to the forest fires problem. P. pinaster by-products were evaluated bearing in mind its wettability and chemical composition (evaluated by FTIR-ATR). Extracts were obtained from the bark, pinecones, and needles using a steam pressure extraction method (2% NaOH)”, a simple extraction method used for the first time in the valorisation of biomass for its application in biomaterials. The structures of resorcinol, catechin and phloroglucinol appear in pine needles, pinecones and bark extracts, respectively. Extracts self-condensation curing process was evaluated by differential scanning calorimetry, and an automatic bonding evaluation system. For the first time particleboards were manufactured at a specific pressure of 4 MPa and 190 °C of press temperature for 3 min, with different percentages of the three maritime pine by-products, using a melamine-urea-formaldehyde resin and citric acid as particle binder. Due to the fast reactivity between polyphenols and formaldehyde, the influence of each by-product on the formaldehyde emission was also studied. The results show that it is possible to improve water resistance and internal bonding strength, and to control formaldehyde emission of particleboards, by using underutilized Maritime pine-based by-products in particleboards, replacing wood particles up to 20 % by weight.

Tannery sludge and urban waste compost in the production of Solanum melongena L. through regression and multivariate analysis

In vegetable production, seedling propagation is one of the most important steps as it directly influences the final performance of the plants. In order for such condition, the propagation substrate must be in good condition. The objective of this work was to evaluate the effect of different combinations of tannery sludge and urban waste compost as substrates in comparison to the commercial substrate Maxfértil® on the growth and quality of eggplant seedlings. The experiment was conducted in a greenhouse using a randomized block design with eight treatments and six replications. The commercial substrate was Maxfértil®, a crushed pine bark, with limestone, and seven treatments with increasing proportions of tannery sludge/decreasing urban waste compost (0/100; 10/90; 30/70; 50/50; 70/30; 90/10 and 100/0 v/v). Characteristics of emergence, growth, quality and leaf nitrogen (N) content were evaluated. No difference was found in the emergence of eggplant seedlings among the different treatments used; however, the growth characteristics, quality and N content featured significant effects when the seeds were propagated in alternative substrates. Regression adjustments pointed to an optimal range of 41–49% of tannery sludge associated with urban waste compost for the evaluated characteristics. The strong correlation between SPAD and N content shows its potentiality in detecting N deficiencies in seedlings. The use of tannery sludge residues associated with urban waste compost seems to be an appropriate and sustainable practice for recycling with high agronomic potential.

Assesment of trap color and trap height above the ground on the capture of Ips sexdentatus and Thanasimus formicarius

The six-toothed pine bark beetle Ips sexdentatus is one of the most devastating bark beetles of Eurasian pine forests. Pheromone traps are used to monitor and control Ips sexdentatus populations. In this study, the effect of trap color and trap height on the capture of Ips exdentatus and its predator the ant beetle Thanasimus formicarius was investigated. The research was conducted in Pinus sylvestris stands within Yayla Forest Enterprise Chief (Kastamonu-Daday) in Turkey. In the study area, 25 Scandinavian type three-funnel traps of 5 different colors (yellow, white, green, black, and red traps with five replications) were used. Traps were placed at the same height, 1.5 m above ground. For the second part of the study, a total of 20 traps (5 per height category) was placed 1.0 m, 1.5 m, 2.0 m, and 2.5 m above ground. Traps were controlled at intervals of 7-10 days and captured Ips exdentatus and Thanasimus formicarius were counted. The results of the study were as follows: 1) Significant differences in the number of captures by trap color, and 2) No significant differences in the numbers of Ips sexdentatus and Thanasimus formicarius captured between the trap heights.

Tree bark characterization envisioning an integrated use in a biorefinery

Tree barks are considerable waste streams in forest industries, and a potentially interesting resource for biorefineries. Barks of six relevant tree species, namely poplar (Populus × canadensis), black locust (Robinia pseudoacacia), red oak (Quercus rubra), willow (Salix sp.), Corsican pine (Pinus nigra subsp. laricio), and larch (Larix decidua), were anatomically and chemically characterized. Anatomical analysis illustrated the structural heterogeneity of the different barks with occurrence of various cell types and their arrangement in phloem, periderm, and rhytidome. Summative chemical analysis showed a high extractive content (14–30 wt%) for all barks. Black locust bark presented a substantial suberin content (10 wt%). The lignin content was similar for most barks (22–29 wt%), except for Corsican pine bark (45 wt%). Analytical pyrolysis demonstrated that softwood bark lignin was mostly G type with a small amount of H units, whereas for hardwood species S, G, and H units were present, with low S/G ratios (0.3–0.7). The cell wall structural polysaccharides were rather low. Total monosaccharides ranged from 35 to 44 wt%, with glucose being predominant for almost all barks, followed by xylose in hardwood and mannose in softwood barks. Identification of the lipophilic extractives highlighted the predominance of resin acids for softwood barks, and fatty acids and triterpenoids for all barks. Analysis of the polar bark extracts revealed large variations in the content of phenolics (265–579 mg gallic acid equivalent/g extract), flavonoids (177–391 mg catechin equivalent/g extract), and condensed tannins (88–670 mg catechin equivalent/g extract). Furthermore, the polar extracts presented a high antioxidant potential (500–1209 mg trolox equivalent/g extract), as determined by the FRAP assay. Additionally, a very strong antioxidant activity (AAI > 2), as evaluated by the DPPH assay, was observed for all barks. In summary, the results highlight the marked anatomical and chemical interspecies variability in barks, thus suggesting the need for tailored biorefining approaches.Graphical abstract

Non-domestic wastewater treatment with fungal/bacterial consortium followed by Chlorella sp., and thermal conversion of the generated sludge.

The online version contains supplementary material available at 10.1007/s13205-021-02780-1.

090 White pine bark extract modulates cell-to-cell communication pathway for a lighten skin and age spot benefit

090 White pine bark extract modulates cell-to-cell communication pathway for a lighten skin and age spot benefit

Valorization of Pinus taeda bark: source of phenolic compounds, tannins and fuel

Pine bark is a byproduct of wood processing which is usually burnt for energy. This article analyzes the liquid–solid extraction of Pinus taeda bark to obtain phenolic compounds by using response surface methodology to determine extraction conditions. The independent variables studied were temperature, ethanol concentration and solid–liquid ratio, and the variables to be optimized were total extractives yield, phenolic content, antioxidant capacity and condensed tannins yield. In addition, the extract was characterized by Fourier transform infrared spectroscopy and UV–Vis spectroscopy. Furthermore, extraction kinetics were modeled, and mass transfer mechanisms were studied. The extraction condition that maximizes extraction yields was defined at 50 °C, with a solid–liquid ratio of 1/10 and with an ethanol concentration of 50%. The condensed tannins yield was 4.01 g catechin equivalent (CE)/100 g pine bark dry base (d.b.), the total extractive yield was 9.83 g extract/100 g pine bark d.b. and the Stiasny number was 77. The extract showed a FRAP antioxidant concentration of 20.89 mmol ascorbic acid equivalent (AAE)/100 g pine bark d.b and 0.35 mmol trolox (TRE)/g pine bark d.b. for ABTS assay. The results showed that extended Fick’s law was adequate to describe the extraction kinetics. The extraction did not significantly affect the calorific value of bark (21 kJ/g d.b.). After extraction, the ashes were reduced by 13% and potassium (K) by 48%. The extraction of Pinus taeda to obtain phenolic content is technically feasible, and this paper provides the scientific ground for further scaling the process.

Blockage of the JAK/STAT3 signaling pathway in multiple myeloma by leelamine

BackgroundLeelamine (LEE) is a lipophilic diterpene amine phytochemical, which can be naturally extracted from pine's bark trees. It has been extensively studied recently for its promising chemopreventive and anti-cancer effects against various cancers such as that of prostate and breast. HypothesisWe examined the potential impact of LEE in affecting the activation of signal transducer and activator of transcription 3 (STAT3) and promoting apoptosis in human multiple myeloma (MM) cells. MethodsWe evaluated the effect of LEE on STAT3 signaling pathway in MM cells by using Western blot analysis and reverse transcription polymerase chain reaction (RT-PCR). Thereafter, apoptosis was evaluated using cell cycle analysis and Annexin V assay. ResultsWe noted that LEE could attenuate the phosphorylation of STAT3 and other up-stream signaling molecules such as JAK1, JAK2, and Src activation in U266 and MM.1S cells. It also diminished STAT3 translocation into the nucleus and enhanced the expression of protein-tyrosine phosphatase epsilon (PTPε). Additionally, LEE caused cell cycle arrest and synergistically augmented the apoptotic actions of bortezomib against MM cells. ConclusionsOur data indicates that LEE could block STAT3 signaling cascade linked to tumorigenesis and can be used in combination with approved anti-cancer agents in attenuating MM growth and survival.

Effects of different antioxidants on quality of meat patties treated with in-package cold plasma

Effects of antioxidants butylated hydroxytoluene (BHT, 0.02%), carnosine (1%), and plant extracts rosemary (RE, 1%), pine bark (PBE, 1%) and pomegranate (PGE, 1%) were investigated on total plate counts, pH, color, and lipid oxidation (LPO) in chicken breast meat patties packed in modified atmosphere and treated with dielectric-barrier-discharge (DBD) cold plasma (CP). The effect on mesophilic inactivation (> 50% reduction) by CP in stored patties varied with antioxidants. No effect was noted for BHT and PBE. RE and PGE enhanced it by >65%, but carnosine suppressed it completely. Antioxidants did not affect CP-induced pH reduction. Plant extracts significantly influenced CIEL*a*b* measurements and prevented CP effects on them during storage. BHT, carnosine, RE, and PGE reduced LPO by more than 18% in CP-treated, stored samples. PBE completely suppressed LPO in CP-treated, and PGE in non-CP-treated, stored patties. Natural plant extracts could improve microbial inactivation and inhibit LPO in CP-processed meat. Industrial relevanceAdding antioxidants in food matrix affects antimicrobial inactivation and prevents oxidative damages by in-package cold plasma (IPCP). The effectiveness varies with the antioxidant. Natural antioxidants rosemary, pomegranate, and pine bark extracts, can be used to enhance antimicrobial inactivation, suppress lipid oxidation, and maintain appearances of IPCP-processed meat products.

MUDAS DA PALMEIRA BURITI TEM BAIXA EXIGÊNCIA HÍDRICA EM VASOS COM SOLO

The swamp palm (Mauritia flexuosa L.f.) is a palm tree that occurs in the Brazilian Amazon, Pantanal and Cerrado biomes and occupies wetlands such as the Cerrado swamps. Because of this association and its high size, swamp palm has been used as an indicator of preserved areas. As it is still explored extractively, little has been investigated about its propagation. Seedlings of 90 days developed from seeds in cylindrical tubes (290 cm3) with substrate (composted pine bark) were prepared. An essay was carried out in a completely randomized design with five treatments, 10 repetitions and one seedling per plot (pot). The maximum water retention capacity (MWRC) of a red dystrophic oxisol, with 20% clay, was estimated in a 12-liter pot. The sifted soil was incubated with lime for 30 days with irrigation for 60% of the MWRC and fertilized with N, P, K, S and B. The treatments were flood, 100%, 75%, 50% and 25% of MWRC. The pots were kept in a greenhouse with plastic cover and irrigated daily to replace the water after weighing. Biometric variables were evaluated after four months: stem diameter, shoot length, fresh and dry mass, shoot root ratio, Dickson quality index and water content. Swamp palms irrigated with 25% of MWRC presented the lowest stem diameter, fresh and dry mass, Dickson quality index and water content. The supply of water with 50% of the MWRC allowed swamp palm seedlings to grow as well as those that received more water: 75, 100% of the MWRC or flood. In conclusion, the swamp palm seedlings (M. flexuosa) developed properly with daily irrigations with 50% of MWRC in 12-liter pot with soil.

Antimicrobial and wound healing properties of cotton fabrics functionalized with oil-in-water emulsions containing Pinus brutia bark extract and Pycnogenol® for biomedical applications.

Topical formulations containing 1-2% of Pinus brutia bark extract and Pycnogenol® have been prepared to investigate the effect of flavonoids on the stability of O/W emulsions, which were subjected to physicochemical and thermal stability tests. The formulations have been applied to cotton fabrics to evaluate antimicrobial properties against Staphylococcus aureus, Escherichia coli, Candida albicans and Aspergillus brasiliensis. Furthermore, prepared cotton fabrics have been tested on keratinocytes seeded in cell culture inserts for wound healing. Results of freeze thaw cycle test indicated enhanced thermo-stability with no major changes in pH and viscosity, likewise the results of centrifugation assay. However, the addition of Pycnogenol® has tremendously decreased the viscosity of the topical formulation (10,900 cp.). In terms of antimicrobial activity, 2% P. brutia treated cotton fabrics decreased the proliferation of Aspergillus brasiliensis 78.8%, which were more effective than that of Pycnogenol® formulation (62.9%). As for wound healing, 2% P. brutia treated cotton fabrics increased HaCaT keratinocyte cell proliferation and accelerated the cell-free gap closure compared to Pycnogenol® and untreated control groups. The obtained results indicate the utilization of pine bark for developing an eco-friendly natural antifungal finish for medical textiles.

Growth, decorative and nutritional values of ornamental cabbage (Brassica oleracea L.) in flowerbed conditions

Ornamental cabbages are cultivars of cabbages grown for decorative purposes due to the varied shape and colour of their internal leaves. The aim of the study was to determine the course of growth and to assess the decorative and nutritional value of two cultivars of ornamental cabbage (‘Pigeon Red’, ‘Coral Prince’) grown in a flowerbed along with annual plants: the French marigold (Tagetes patula nana L.) and flossflower (Ageratum houstonianum Mill.), using a pine bark mulch. The impact of the marigold and flossflower on the cabbage height and foliage changed with the plant growth. Cabbages growing without the proximity to the other species had the smallest diameter and formed the least leaves; and the plant’s height was lower in September. The cabbage height, foliage and leaf colour were modified by the weather in the subsequent years of research. The proximity of the marigold or flossflower and mulching of the soil with bark did not affect the colour of the leaves. The nutritional value of the ornamental cabbage was high, and the content of the mineral components in the leaves depended largely on their colour. The coloured leaves were more abundant in nitrogen, phosphorus, potassium and magnesium, while the green leaves contained more calcium and sulfur, and slightly more dry matter.

The Potential Effect of Pests on Forest Fire: Flammability of Mongolian Pine Bark with Resinosis on Boles

Wildfires and pests are natural disturbance agents in many forest ecosystems that often contribute to ecological succession, nutrient cycling, and forest species composition. Mongolian pine (Pinus sylvestris var. mongolica) is a coniferous species that plays an important role as an ecological barrier, and is widely spread in northern China. Its wood is loose; its branches, leaves, and cones contain a high level of resin and volatile oils that make the species highly flammable and the stands dominated by the species are very vulnerable to fire. Recently, resinosis on boles of Pinus sylvestris became an epidemic in China. To explore the potential effects of pests on fire, we compared the flammability of Mongolian pine barks with or without resinosis on boles using a cone calorimeter and several combustion analyses. We found that the barks from boles with resinosis had a greater oil content than the healthy trees. The study also indicated that the ignition times of the barks from boles with and without resinosis were 6.00 s (±1.73) and 22.67 s (±1.15), respectively, and that the heat release rate curves were parabolic, with peaks 225.19 and 75.27 kW/m2, respectively, for the two bark types. Additionally, because resinosis was on the low- to mid-bole of infested trees, the barks from boles with resinosis tended to be ignited much easier than those without resinosis. This clearly evidenced that pests could affect fire severity and behavior by increasing forest flammability. More information about the role that pests play in the different forest cover types is needed to increase our understanding of fire danger and to develop sound forest management policies.

SYNTHESIS AND STUDY OF THE PROPERTIES OF XEROGELS DERIVED FROM SULFATED PINE ETHANOL LIGNIN

Organic xerogels based on lignin and tannins isolated from pine bark and wood were first obtained by condensation with formaldehyde and furfuryl alcohol in the presence of hydrochloric acid. The use of pine sulfated ethanol lignin made it possible for the first time to obtain sulfur-containing (up to 1.3% wt.) lignin-(tannin)-formaldehyde and lignin-(tannin)-furfuryl xerogels. The density of the obtained gels increases with the addition of tannins to lignin and varies in the range 0.13–0.39 g/cm3. Xerogels synthesized by condensation with furfuryl alcohol are stronger than those obtained using formaldehyde. The presence of sulfur in xerogels was confirmed by elemental and chemical analysis and IR spectroscopy. It was shown by scanning electron microscopy, that lignin-formaldehyde xerogels are formed from large polymer chains, consisting of interconnected aggregates of micron-sized particles and have large pores. The addition of tannins to the polycondensation system is accompanied by the formation of a more compact spatially crosslinked gel structure. BET method showed that all xerogels have low porosity, and lignin-furfuryl samples have a larger average pore diameter (7.2–14.5 nm) compared to lignin-formaldehyde samples (3.03–6.80 nm).

Environmental Aspect of the Cement Manufacturing in the Świętokrzyskie Mountains (Southeastern Poland)

The research area located in south-eastern Poland is exposed to alkaline pressure from a cement plant in Nowiny, which has been operating there since 1960. Both biotic and abiotic components of the natural environment remain in the zone of the plant’s negative impact. Their reaction, described according to the concept of geo- and bioindicators, provides valuable information on the state of the natural environment in this area. In 2016–2018, the properties of precipitation were being investigated by determining its pH, EC (specific electrolytic conductivity), and chemical composition in terms of the presence of selected heavy metals. Additionally, the chemical composition of samples of two-year-old pine needles, bark, and wood, as well as soil taken from the immediate vicinity of the cement plant, was analyzed. The analysis performed using the Inductively Coupled Plasma-Mass Spectrometer-Time-of-Flight (ICP-MS-TOF) showed the presence of heavy metals. The highest contents were recorded in soils (Pb 123 mg·kg−1; Cr 138 mg·kg−1; Cu 18 mg·kg−1; Zn 699 mg·kg−1), while the lowest ones were found in the wooden cores of drilled trees (Pb 0.1 mg·kg−1; Cr 1 mg·kg−1; Cu 0.1 mg·kg−1; Zn 4.1 mg·kg−1). On the basis of the research conducted on precipitation, it was found that the pH and EC values were increased, and the presence of heavy metals (Pb, Cr, Ni, and Cu) indicating a local source of pollution was indicated.

A Placebo-Controlled, Pseudo-Randomized, Crossover Trial of Botanical Agents for Gulf War Illness: Curcumin (Curcuma longa), Boswellia (Boswellia serrata), and French Maritime Pine Bark (Pinus pinaster).

This report is part of a larger study designed to rapidly and efficiently screen potential treatments for Gulf War Illness (GWI) by testing nine different botanicals. In this placebo-controlled, pseudo-randomized, crossover clinical trial of 20 men with GWI, we tested three botanical agents with putative peripheral and central anti-inflammatory actions: curcumin (Curcuma longa), boswellia (Boswellia serrata), and French maritime pine bark extract (Pinus pinaster). Participants completed 30 +/− 3 days of baseline symptom reports, followed by 30 +/− 3 days of placebo, 30 +/− 3 days of lower-dose botanical, and 30 +/− 3 days of higher-dose botanical. Participants then repeated the process with a new botanical until completing up to three botanical cycles. Data were analyzed using linear mixed models. Curcumin reduced GWI symptom severity significantly more than placebo at both the lower (p < 0.0001) and higher (p = 0.0003) dosages. Boswellia was not more effective than placebo at reducing GWI symptoms at either the lower (p = 0.726) or higher (p = 0.869) dosages. Maritime pine was not more effective than placebo at the lower dosage (p = 0.954) but was more effective than placebo at the higher dosage (p = 0.006). This study provides preliminary evidence that curcumin and maritime pine may help alleviate symptoms of GWI. As a screening study, a final determination of the efficacy of these compounds for all individuals with GWI cannot be made, and further studies will need to be conducted to determine strength and durability of effects, as well as optimal dosage. These results suggest that GWI may, at least in part, involve systemic inflammatory processes. This trial was registered on ClinicalTrials.gov (NCT02909686) on 13 September 2016.

Potencial dos substratos pó-de-coco e casca de pinus compostada na promoção de crescimento de mudas de umbuzeiro

The objective of this work was to evaluate the potential of the processed coconut fiber (coconut powder) and composted pine bark, isolated and in combination, in promoting the growth of umbuzeiro seedlings. This work was conducted in a greenhouse in a completely randomized block experimental design, with five treatments and four replications. The treatments consisted of the following formulations, in volumetric proportions: 100% pine bark (CP); 75% CP + 25% coconut powder (PC); 50% CP + 50% CP; 25% CP + 75% CP and; 100% PC). The coconut powder substrates or the formulation containing 75% coconut powder + 25% composted pine bark provided Imbu seedlings with higher vegetative quality. The lower density, higher total porosity and higher percentage of available water were the physical characteristics of the substrates that most influenced the growth of Imbu seedlings.

Corticioid basidiomycetes associated with bark beetles, including seven new Entomocorticium species from North America and Cylindrobasidium ipidophilum, comb. nov.

Seven new Entomocorticium species (Peniophoraceae) are described based on morphology and phylogenetic analyses. Along with the type species (E. dendroctoni), Entomocorticium comprises eight species of nutritional symbionts of pine bark beetles in North America. Entomocorticium cobbii is the mycangial associate of the southern pine beetle, Dendroctonus frontalis, and E. parmeteri is the mycangial associate of the western pine beetle, D. brevicomis. Entomocorticium whitneyi, E. portiae, E. kirisitsii, E. oberwinkleri and the previously described E. dendroctoni have been isolated from galleries of D. ponderosae and D. jeffreyi in western North America. Entomocorticium sullivanii forms an ambrosia-like layer of basidia and basidiospores in the pupal chambers of Ips avulsus in the southeastern USA. Entomocorticium is phylogenetically placed within Peniophora, a corticioid genus of wood decay fungi with wind-dispersed basidiospores. At least four species of Entomocorticium produce basidiospores on basidia with reduced sterigmata that apparently do not forcibly discharge basidiospores. Another basidiomycete, Gloeocystidium ipidophilum, was described from Ips typographus galleries in Europe, but it is phylogenetically and taxonomically placed in another genus of wood decay fungi as Cylindrobasidium ipidophilum (Physalacriaceae). The free-living wood-decay fungus Phlebiopsis gigantea (Phanerochaetaceae) has been occasionally associated with bark beetles but is unrelated to C. ipidophilum or Entomocorticium.

Investigation of Phenolic Content in Five Different Pine Barks Species Grown in Turkey by HPLC-UV and LC-MS.

Investigation of phenolic content from different pine bark species grown in Turkey was performed using a reversed-phase high pressure liquid chromatography with ultraviolet (RP-HPLC-UV) method. All phenolic constituents were separated in <26min on reversed-phase C18 column with gradient mobile phase that consists of orthophosphoric acid, methanol and acetonitrile. Detections were made on an UV detector at 280nm and at a flow rate of 1mL/min. Samples were prepared according to Masqueller's conventional sample preparation method with slight modifications. To avoid the reduction in extraction efficiency the sample preparation step was carried out under argon atmosphere. The linearity of the method was between 0.9994 and 0.9999. The detection limits for the five phenolic constituents ranged from 0122 to 0.324mg/L. Catechin and taxifolin were found in all pine barks at a concentration of 0.065±0.002-1.454±0.004 and 0.015±0.001-23.164±0.322mg/g, respectively. Epicatechin was determined in four pine barks between 0.027±0.001 and 0.076±0.002mg/g, ferulic acid in two pine barks between 0.010±0.001 and 0.022±0.001mg/g and epicatechin gallate in only one of the pine barks at 0.025±0.001mg/g. Finally, the total amount of phenolic compounds and antioxidant capacities of the pine barks were found to be very high.