Physiological Norm Research Articles

Evaluation of electrognathography indicators in patients with decreased height of the lower part of the face and symptoms of intolerance to the structural materials of dentures

Purpose of the study: to conduct a comparative assessment of electrognathography indices in elderly patients with a decrease in the height of the lower part of the face with symptoms characteristic of intolerance to the structural materials of dentures, before and during the diagnostic and treatment stages of using occlusal-stabilizing devices. Material and methods. We examined 76 patients aged 61 to 74 years with a decrease in the height of the lower part of the face and the presence of symptoms characteristic of intolerance to the structural materials of dentures. Based on the results of a short “Hamburg” examination, 30 patients were selected with the possibility of dysfunctional disorders of the temporomandibular joint, for whom occlusal-stabilizing devices were made. Results. A study of elderly patients with a decrease in the height of the lower part of the face and symptoms characteristic of intolerance to the structural materials of dentures revealed the effectiveness of the use of occlusal-stabilizing devices at the treatment and diagnostic stages due to a decrease in complaints of dry mouth more than in twice, a decrease in the intensity of burning of the mucous membrane, a decrease in the number of complaints accompanying dysfunctional disorders of the temporomandibular joint, as well as an improvement in electrognathography indicators to physiological norms. Conclusion. The obtained results of treatment with occlusal-stabilizing devices for patients with a decrease in the height of the lower part of the face, presenting complaints characteristic of intolerance to the structural materials of dentures, at the treatment and diagnostic stages indicate the feasibility and effectiveness of their use in this category of people.

Evaluation of the efficiency of the hepatoprotector glucuronate in toxic hepatitis of various etiology

The objective was to study the effectiveness of the use of hepatoprotector glucuronate at the early stage of intensive therapy of toxic hepatitis of various etiologies.Materials and methods. We studied 120 patients with toxic hepatitis who were treated at the Republican research center for emergency medicine in 2022–2023. The study was carried out in two groups of patients. Group I – 60 patients admitted in 2023, who, in addition to traditional therapy, received a complex hepatoprotector glucuronate (Jetepar®)*. Group II (comparison group) – 60 patients who applied in 2022 and received only traditional therapy. Biochemical blood parameters were studied upon admission and over time on day 5. The severity of intellectual impairment was assessed using cognitive scales and the Reitan test on days 2 and 5 from the start of treatment.Results. Upon admission, both groups showed signs of toxic liver damage. In dynamics by the 5th day in patients of group I, there was a decrease in ALT by 63.7%, AST by 66.4%, alkaline phosphatase by 54.2% from the initial values, which was 3.9 and 2.6, 2. 1 lower than in the comparison group. The level of free ammonia in patients of group I on the 5th day decreased by 52%, and lactate by 57% from the initial level, almost to the physiological norm, while in the comparison group (II) only by 24.8% and 38.1%, which was 2.2 and 1.5 times worse than in the main group. Screening of the level of intelligence using two cognitive scales and the Reitan test showed that in patients in the comparison group, cognitive function indicators on the 5th day were 1.4, 1.5 and 1.2 times lower than in the main group.Conclusion. The use of the hepatoprotector glucuronate improves liver parameters and cognitive functions in patients with toxic hepatitis.

CHANGES IN LIPID AND CARBOHYDRATE METABOLISM IN PATIENTS WITH OSTEOARTHRITIS WITH ACCOMPANYING ARTERIAL HYPERTENSION AND TYPE 2 DIABETES, DEPENDING ON BSMI VDR GENE POLYMORPHISM

It is known that Osteoarthritis (OA) is a common chronic disease of the musculoskeletal system, which leads to a significant loss of working capacity and disability of people of working age, which worsens the quality of life of the population and causes economic burden and losses to all countries of the world. In addition, OA is known as a disease with an inherent high level of comorbidity. Scientists single out diseases of cardiovascular diseases, digestive systems and metabolic disorders as those that are most often combined with OA and complicate the course of each other in manifestations, diagnostic and treatment processes. Today, modern science needs to understand the presence of a common genetic origin. It is known that the polymorphic variant of the VDR vitamin D receptor gene has a predisposition to common osteochondral diseases. However, the issue of participation in pathogenetic mechanisms of development and progression remains controversial. A polymorphic variant of the VDR gene has a predisposition to osteochondral diseases. However, its involvement in the pathogenetic mechanisms of the development and progression of osteoarthritis remains controversial, therefore The aim of the study is to investigate the specifics of the impact of comorbid pathology on carbohydrate and lipid metabolism and the state of the vascular wall in patients with osteoarthritis, taking into account the polymorphic variant of the VDR gene (Bsml, rs1544410). Object and research methods. 100 patients were examined and also divided into groups: 1st group – patients with OA; II – OA with arterial hypertension; ІІІ – OA, arterial hypertension (AG) and type 2 diabetes mellitus, a blood sample was taken and, depending on age, comorbidity, the presence of the polymorphic variant BsmI (rs1544410) of the VDR gene, indicators of carbohydrate and lipid metabolism, the level of end metabolites of nitric oxide and endothelin-1 were studied and the impact on radiological changes and the functional state of the knee joints was evaluated. Control – 30 people. Results of the study and their discussion. It has been established that osteoarthritis is characteristic of persons of working age. With increasing age, it is characteristic to be burdened by comorbid pathologies with worsening of the course of the main disease. Expected violation of carbohydrate metabolism was observed in the III group of patients, although dynamic probable changes were also in the II group (p<0.05). Lipid metabolism in the I group was probably higher than the group of control, but was within the physiological norm, while groups II and III had significant pathological changes compared to the indicators of the I group and practically healthy persons. Hypercholesterolemia became a trigger for a decrease in the total level of stable total nitrogen monoxide metabolites and an increase in endothelin-1, which forms endothelial dysfunction and leads to the progression of AG and worsening of the course of OA. After the genotyping and division into genotypes of the polymorphic variant BsmI of the VDR gene, conclusions were obtained about the unfavorable allele A in the exchange processes of the above indicators. Conclusions. Features of violations of carbohydrate and lipid metabolism, the condition of the endothelium, against the background of comorbidity and the presence of an unfavorable allele A of the BsmI polymorphic variant of the VDR gene should be taken into account in the complex diagnostic and treatment process of patients with OA.

Typical Pathological Process in Glutamate Neurotoxicity: the Role of Reactive Nitrogen and Oxygen Species

The disruption of major regulatory mechanisms of nerve cells can lead to glutamate neurotoxicity/excitotoxicity. This phenomenon most often occurs in hypoxia/ischemia, during inflammatory processes, activation of immune and autoimmune responses. Pathological changes in the brain in the early stages of diseases are nonspecific. The distinction between pathological change and physiological norm is small. These typical pathological processes are common with a variety of diseases. They may also occur in ischemic and hemorrhagic strokes. The main objective of this work was to analyze some physiological and cytochemical processes that are associated with the neurotransmitter glutamate, as well as with highly reactive and highly toxic compounds such as reactive nitrogen and oxygen species. Reactive nitrogen (•NO) and oxygen (•O2–) species can affect almost all major components of cells and subcellular structures. At low concentrations, they perform a regulatory function. Mechanism analysis of toxic effects of glutamate, reactive nitrogen and oxygen species was essential for the development of new methods of protection against the damaging effects of the said substances thereby using these methods in treatment of ischemic and hemorrhagic strokes.

Nitrogen metabolism in blackmoiled cattle of Kuzbass

A research analysis of nitrogen metabolism in black-and-white cattle was carried out in the conditions of the Kuzbass region. Healthy animals at the age of 2nd lactation with a productivity level from 7300 to 10500 kg were selected for the experimental group. Laboratory studies were carried out at the Department of Veterinary Genetics and Biotechnology of the Novosibirsk State Agrarian University. Indicators of nitrogen metabolism in blood serum were assessed photometrically on a biochemical semi-automatic analyzer Photometer 5010 V5+. Reagent kits from Vector-Best and Olvex-Diagnosticum were used. When biometric data processing, standard descriptive statistics techniques and robust indicators were used. The Anderson-Darling test was used to assess the normality of data distribution. .When calculating correlation relationships, the Pearson coefficient was used. Calculations were performed in the “RStudio” programming environment. The obtained average values of nitrogen metabolism indicators were within the physiological norm. The strongest positive associations were observed between urea levels and uric acid, and urea and creatinine. No connection was found between other indicators of nitrogen metabolism. There were no differences in milk yield between cows with high and low levels of nitrogen metabolism. Graphs of the range of nitrogen content in black-and-white cows during lactation are presented. Calculations of reference indicators based on data from Kuzbass dairy cattle make it possible to more accurately assess the clinical condition of animals and timely detect deviations from established standard values. It is also necessary to take into account reference limits when monitoring the quality and completeness of cattle.

Нелинейные главные компоненты биохимического профиля молочного скота пяти пород

Abstract. The purpose of this study was to identify and explain patterns of biochemical parameters in dairy cows, taking into account the characteristics of their breed. Methods. A comparison was made of samples of animals of five breeds (Holstein, Tagil, Suksun, Sychevsk, Istoben) according to 17 biochemical parameters. The total number of animals studied was 407. To analyze the obtained results, statistical analysis methods were used, including the method of nonlinear principal component analysis using the CATPCA (Categorical PCA) algorithm. Scientific novelty. The chosen method made it possible to summarize a large number of biochemical indicators and identify processes in which the studied groups of cows differed to a greater or lesser extent. Results. Five principal components were identified and interpreted, explaining a total of 67.4 % of the total variance. Some observed patterns may indicate the development of pathological conditions in animals. Similar biochemical patterns were observed, on the one hand, in cows of the Tagil, Holstein and Suksun breeds, and on the other, in the Istoben and Sychev breeds. The animals of the Suksun breed were closest to the physiological norm. More pronounced changes associated with a negative energy balance were observed in Holstein cows.

Biochemical and morphological blood parameters and the level of natural resistance of imported cows in Armenia

The article presents the results of a comparative study morphological and biochemical features of blood and indicators natural resistance of imported Holstein cows, Simmental and brown Swiss cows, in the conditions of the farm LLC “Agroholding Armenia” near the city of Spitak, mountainous area of the Republic Armenia. For the purpose of conducting the experiment, 3 groups of animals were formed I – Holstein, II – Simmental and III – brown Swiss, 15 heads each one. The groups were compiled using the method of analog groups. Conditions the contents and feeding were the same and in accordance with the accepted According to the technology, the cows had an average fatness. The results of our research have been confirmed, what are the fluctuations morphological and biochemical parameters of the blood of the bred farms of imported breeds have minor difference from existing physiological norms, but according to the content of cow globulin groups II and III were inferior to the norm, which may be due to pedigree features in the existing conditions of feeding and maintenance. Hematological date and indicators of natural resistance they testify to the best adaptive qualities and adaptive the possibilities of imported animals.

Optometrische Vision Therapy

Purpose. Vision therapy in a 26-year-old female student with subjective vision impairment because of reduced flexibility of accommodation, esophoria, and lack of divergence. Material and Methods. Regular vision therapy based on a detailed training concept with training under optometric supervision and home exercises was performed over a period of twelve weeks. The baseline examination included history as well as analysis of the visual system with optometric function tests, refraction, and integrative analysis. The training progress was controlled biweekly. Results. The parameters that differed from the physiological norm data at baseline increased as follows: maximum amount of accommodation OD/OS from 6.7/7.7 D to 8.0/8.7 D; flexibility of accommodation monocular OD/OS from 6/6 to 13/12 turns per minute, binocular from 7 to 13 turns per minute; positive/negative relative accommodation from −0.75/+1.25 D to −1.75/+1.75 D; vergence ranges base in distance/near (break points) from 3/6 cm/m to 6/10 cm/m. Conclusion. All described parameters were improved after vision therapy. The accommodation flexibility and positive and negative accommodation were increased to the physiological norm range. The subjective complaints, such as headaches and rapid fatigue when reading, were eliminated by the training. Keywords: VISION THERAPY, CONSTANT ESOPHORIA, REDUCED ACCOMMODATION FLEXIBILITY, LACK OF DIVERGENCE

Adaptation Potential and Body Mass Index in Assessing the Vital Activity of the Population in Mountain Regions.

We aimed to evaluate the functional state of the population of the midlands and highlands of Alai region (Kyrgyzstan) on the basis of body mass index (BMI) and adaptation potential (AP). The characteristic of people with overweight and underweight are presented. A clean dependence of AP on BMI was revealed. In Alai region (Kyrgyzstan), 285 citizens of Gulcha village (1.540 m above sea level) and 312 citizens of Sary-Tash village (3.200 m above sea level) were examined in July-August 2023. Blood pressure (BP) was generally measured at rest in the seated position. The BMI and AP were calculated. In the midlands and highlands BP correspond to the physiological norm. The AP scores in the midlands region for men was 2.32 points, for women - 2.38 points, but in highlands areas these values were slightly higher: for men - 2.52 points, for women - 2.42 points. The total BMI values for men in the midlands and highlands were 23.39 and 23.24 kg/m2 respectively, for women in the midlands this indicator became higher than normal (26.53 kg/m2), but in the highlands it corresponded to the norm (24.78 kg/m2). It is necessary to determine the value of AP, BMI and type of self-regulation of blood circulation when assessing the functional state of residents living in mountain areas. In the highlands overweight, which is one of the significant risk factors for health disorders, with tension of regulatory mechanisms can become a trigger for the development of cardiovascular diseases.

ИЗМЕНЕНИЕ МИНЕРАЛЬНОГО СОСТАВА МОЛОКА КОРОВ ПОД ВЛИЯНИЕМ БРИКЕТОВ-ЛИЗУНЦОВ

The aim of the study is to investigate the degree of influence of environmentally friendly lick briquettes Amirasol G(S)-Z and Amirasol G(S)-L on the mineral composition of milk, milk productivity and milk fat content of Caucasian brown cows of the mountain biogeochemical province of the Republic of Dagestan in the autumn-winter and spring-summer periods of keeping. During the use of lick briquettes in the diets of cows during the year, their positive effect on the mineral composition of milk, milk productivity and milk fat content was revealed. Moreover, a reliable increase was established in the milk of the experimental group of cows in the concentration of macroelements potassium, sodium, magnesium, calcium, phosphorus, respectively, by 12.17; 155.10; 52.82; 46.18; 93.96 %; microelements iron, zinc, manganese, copper, cobalt, iodine (CBI), selenium, respectively, by 101.84; 113.96; 256.01; 130.47; 265.85; 530.0; 275.91 % compared to the control group, compared to the average level of the physiological norm – by 19.66; 39.70; 5.84; 82.12; 92.96; 98.94; 83.17; 80.11; 94.08; 47.67; 210.89;109.73 %. In the control group of cows, the concentration of macro- and microelements was below the lower limit of the norm, with the exception of potassium and manganese, which were higher by 29.01 and 10.04 %, respectively, compared to the lower limit of the physiological norm. The use of lick briquettes in the diets of dairy cows during the year also contributed to an increase in milk productivity by 6.23 %, milk fat content by 5.0 % (0.20 abs.%), an additional 0.49 liters of milk per head per day were obtained on average for the group. The economic effect was 80,460 rubles, or 2,682 rubles per head during the experiment.

Показатели крови перепелов при скармливании муки из высушенных личинок Lucilia Caesar

The aim of the research was to study the effect of compound feed with the addition of flour from dried larvae of the Lucilia Caesar fly on the morphological and biochemical parameters of the blood of young quails. The experiment was conducted on 160 Phoenix quail at the age of 10 days, of which two groups were formed according to the principle of balanced groups. The results of the tests showed that all the studied blood parameters of the experimental bird were within the physiological norm. When feeding compound feed with a protein supplement from flour of the Lucilia Caesar larva, the content of albumins in the blood serum of quails of the second group decreased by 2.0 g/l (p≤0.05)., globulins - by 1.0 g/l (p≤0.05), urea - by 0.24 mmol/l (p≤0.01). Probably, a decrease in albumins and urea in the blood of quails of the second group indicates that they are involved in protein synthesis. Gamma-glutamyltransferase (GGT) is an enzyme involved in amino acid metabolism. Catalyzes the transfer of a gamma - glutamyl residue from a gamma-glutamyl peptide to an amino acid, another peptide, or, during hydrolysis, to water. The activity of the enzyme in the blood increases in liver diseases. In our experiment, a decrease in the GGT content by 2.66 u/l in the blood of a bird receiving flour from the larvae of the Lucilia Caesar fly probably indicates an activation of amino acid synthesis in the body, which is quite consistent with the data of the average daily increments of the experimental bird. Lactate dehydrogenase (LDH) in the blood is an enzyme localized inside cells. The composition necessarily includes zinc ions. The main function is to catalyze the oxidation of lactic acid to pyruvate. In diseases accompanied by tissue damage and cell destruction, LDH activity in the blood increases. In this regard, it is an important marker of tissue destruction. According to our data, LDH levels in the blood of the experimental bird of the second group significantly decreased by 2909.75 mmol/l (p≤0.05), which indicates an improvement in the functional activity of the liver.

Показатели крови перепелов при скармливании муки из высушенных личинок Lucilia Caesar

The aim of the research was to study the effect of compound feed with the addition of flour from dried larvae of the Lucilia Caesar fly on the morphological and biochemical parameters of the blood of young quails. The experiment was conducted on 160 Phoenix quail at the age of 10 days, of which two groups were formed according to the principle of balanced groups. The results of the tests showed that all the studied blood parameters of the experimental bird were within the physiological norm. When feeding compound feed with a protein supplement from flour of the Lucilia Caesar larva, the content of albumins in the blood serum of quails of the second group decreased by 2.0 g/l (p≤0.05)., globulins - by 1.0 g/l (p≤0.05), urea - by 0.24 mmol/l (p≤0.01). Probably, a decrease in albumins and urea in the blood of quails of the second group indicates that they are involved in protein synthesis. Gamma-glutamyltransferase (GGT) is an enzyme involved in amino acid metabolism. Catalyzes the transfer of a gamma - glutamyl residue from a gamma-glutamyl peptide to an amino acid, another peptide, or, during hydrolysis, to water. The activity of the enzyme in the blood increases in liver diseases. In our experiment, a decrease in the GGT content by 2.66 u/l in the blood of a bird receiving flour from the larvae of the Lucilia Caesar fly probably indicates an activation of amino acid synthesis in the body, which is quite consistent with the data of the average daily increments of the experimental bird. Lactate dehydrogenase (LDH) in the blood is an enzyme localized inside cells. The composition necessarily includes zinc ions. The main function is to catalyze the oxidation of lactic acid to pyruvate. In diseases accompanied by tissue damage and cell destruction, LDH activity in the blood increases. In this regard, it is an important marker of tissue destruction. According to our data, LDH levels in the blood of the experimental bird of the second group significantly decreased by 2909.75 mmol/l (p≤0.05), which indicates an improvement in the functional activity of the liver.

Influence of the Glutaraldehyde-based and Quaternary Ammonium Compounds Based Aerosol Disinfectant on Some of the Physiological and Zootechnical Indices in Broiler Chickens

Introduction. A huge number of disinfectants are used for aerosol disinfection of poultry premises, most of them are recommended for preventive or terminal disinfection carried out in the absence of poultry. At the same time, some products available to veterinary services are recommended for concurrent disinfection and are used in the presence of poultry, although, not all of them meet the requirements on composition, purpose and mode of use. The one of interest is a glutaraldehyde-based and quaternary ammonium compounds (QAC) based disinfectant, which has already been investigated in the scientific sources, however the influence of this agent on the physiological and zootechnical status of poultry treated therewith has not been fully studied. The aim of this research is to study the physiological status and productive qualities of the broiler chickens exposed to direct aerosol treatment with the glutaraldehydeand QAD based disinfectant.Materials and Methods. The research was conducted from October to November 2019 in the industrial poultry farm settings in a large flock of broiler chickens of the Cobb 500 cross, divided into two groups — experimental and control one. In the experimental poultry house, on the 10th day after putting the broiler chickens in the house, a preventive aerosol disinfection was carried out by means of the Nebulo cold fogger in the presence of poultry. A 0.5% solution of glutaraldehydeand QAD based disinfectant was used in a dosage of 5 ml per 1 m3 (the maximum permissible concentration exceeding the recommended one). In the control poultry house the aerosol disinfection was not carried out. The biochemical tests of blood serum (alanine aminotransferase (ALT), aspartate transaminase (AST), total bilirubin, total protein, creatinine, blood urea, blood uric acid) were made using a biochemical analyser. The zootechnical indices were also studied (livability, mortality, average daily gain, feed and water consumption during the full growing life cycle, feed conversion ratio).Results. The time history of the blood serum biochemical indices showed that the use of the glutaraldehydeand QAD based aerosol disinfectant in the specified above dosage in the presence of broiler chickens did not cause a negative, toxic effect on chickens’ metabolic indicators — all changes fluctuated slightly within the physiological norm. The zootechnical monitoring data have revealed the positive effect of the aerosol disinfection on the growth and maturing of chickens, enhancement of the livestock livability and poultry average daily gain due to the decrease of microbial burden on the growing organisms of chickens. The broiler chickens livability during the entire growing period in the experimental group was 0.84% higher than in the control group. Also, the average daily gain by the end of the growing period in the experimental group was 1.52% higher than in the control group, and the average liveweight of one bird was 2 137.7 g (experimental group) and 2 111.1 g (control group). At the same time, the feed costs in the experimental group were 0.11% less than in the control group.Discussion and Conclusion. It was found that the use of a glutaraldehydeand QAD based aerosol disinfectant, even in the maximum permissible concentration in the presence of poultry, not only didn’t have a negative effect on the physiological and zootechnical status of the broiler chickens, but, on the contrary, fostered creation of the more comfortable conditions for their keeping and growing. The obtained research results allow us to confidently recommend this method of aerosol disinfection to be used in the animal and poultry keeping premises.

Registration of jaw centric relation with different deprogramming devices

BACKGROUND: There are several methods to register jaw centric relation for diagnosis and therapy of dysfunctional disorders of the temporomandibular joint. However, there is insufficient information in the literature regarding the choice of the optimal way to determine this indicator. AIM: To compare different methods for recording jaw centric relation according to the data of the computed tomography of temporomandibular joint. MATERIALS AND METHODS: The study includes participants with increased abrasion of hard tooth tissues and symptoms of musculoskeletal dysfunction of the temporomandibular joint. The main group (80 people) has been recorded jaw centric relation using deprogramming devices (Lucia jig — 1stsubgroup, Kois deprogrammer — 2ndsubgroup, sheet calibrator — 3rd subgroup, deprogrammer in combination with M. Rocаbado kinesiotherapy elements — 4th subgroup). The control group included 20 participants, who had not had deprogramming before registration of jaw centric relation. The results were monitored using computed tomography of temporomandibular joint and subsequent assessment of the sizes of the TMG articular gap in the coronary and sagittal sections at 3 stages of the study: diagnostic, after registration of jaws central relation and prosthetic treatment and 6 months after prosthetic rehabilitation. Since there were no more than 50 observations in each group, the Shapiro–Wilk W-test was used to verify compliance with the norm. RESULTS: According to the data obtained, the parameters of the temporomandibular joint gap have changed in the main group of the 1th subgroup, where indicators are approaching the physiological norm (98% of cases). In the 2ndsubgroup, 90% of the participants show a tendency to conform to the physiological norm. The exception is 10% of the participants with no tendency of improvement. The smaller part (75%) of the participants of the 3rd subgroup observed compliance of the parameters of the articular gap with the criteria of the norm. The dimensions of the anterior part of the articular gap of the temporomandibular joint exceed the dimensions of the distal part, which indicates the anterior position of the temporomandibular joint. Some patients in this group (25%) showed no positive results of computed tomography measurements after jaws centric relation registration. In the 4th subgroup, the patients returned to normal values (100% of the cases). The positive effect of prosthetic treatment and restoration of the position of the articular heads of the temporomandibular joint were obtained in 45 % of the participants in the control group. However, in 55% of the participants the location of the articular gap of the temporomandibular joint did not improve. CONCLUSIONS: Lucia jig in combination with the M. Rokabado’s complex of cranial-postural kinesiotherapy is the most effective method to register jaw centric relation within this study. The remaining methods are also applied among prosthetic dentists and gnathologists. However, in each clinical situation, it is necessary to consider temporomandibular joint pathology indications, type and degree of severity as well as the technical and financial capacity of both doctor and patient.

ИЗУЧЕНИЕ ЭЛЕМЕНТНОГО СОСТАВА ПОБЕГОВ СМОРОДИНЫ ЧЕРНОЙ И ЭКСТРАКТА ЛИСТЬЕВ И ПОБЕГОВ СМОРОДИНЫ СУХОГО

Aim. The study of new promising types of plant raw materials in order to establish the possibility of its further use in medicine is one of the priorities. Numerous studies have shown the presence of a rich elemental composition of plant raw materials, which can be used in medical and pharmaceutical practice to create phytopreparations and biologically active additives to correct the physiological norms of the content of elements in the human body [1]. In this regard, a comparative study of the elemental composition of black currant shoots and extract of leaves and shoots of dry currant is of practical interest for the further development of dosage forms and the formation of a scientific justification for their use in medical practice. The qualitative and quantitative composition of the elements of black currant shoots and extract of leaves and currant shoots was studied using X-ray fluorescence spectrometry on the X-ray fluorescence spectrometer of the QUANT'X brand of Thermo Scientific company. The conducted studies have shown the identity of the composite elemental composition of black currant shoots and extract of leaves and shoots of dry black currant. It has been established that in the shoots of black currant and in the extract of leaves and shoots of dry currant, elements accumulate in significant amounts: potassium, calcium, sodium, phosphorus, magnesium and iron, which is of interest for further development and production of medicines or biologically active additives based on them. Black currant shoots and extract of leaves and shoots of dry currant can act as sources of biologically available macro-, micro- and ultra-trace elements necessary for the human body.

Biochemical indicators of pig blood when using a complex probiotic feed supplement

The use of probiotics in animal feed is one of the most promising alternatives to the application of antibiotics. In order to establish the effectiveness of the use of a complex probiotic supplement (Lactobacillus spp., Lactococcus spp., Spirulinaceae, Saccharomyces cerevisiae, Actinomycetaceae, Aspergillus spp. and Penicillium spp.) in pig farming, two groups of pigs of the Poltava Meat breed were formed by the analog method. In the ration of the fattening pigs of the experimental group, 1.0% of the mass of compound feed was replaced with a complex probiotic supplement, which was fed during the 45 days of the experimental period at the age of 135 to 180 days. In experimental pigs, biochemical blood parameters were determined (the content of protein, lipids, cholesterol, triglycerides, activity of aspartate aminotransferase and alanine aminotransferase, the content of creatinine, glucose, calcium, phosphorus, albumins and globulins) using test kits from the company "Filisit Diagnostics" (UA). As a result of the research, it was established that with the use of a complex probiotic feed supplement, the content of total protein in the blood serum of the pigs of the experimental group was higher by 18.4%, glucose by 21.4%, aspartate aminotransferase activity by 20.4%, alanine aminotransferase by 38.0% compared to control group. In both groups, regardless of the established difference, the named indicators were within the physiological norm. The concentration of cholesterol in the blood of pigs receiving the probiotic supplement decreased by 43.2%, but the concentration of calcium (by 26.2%) and phosphorus (by 17.9%) increased. In the experimental group, the blood indicators related to the immunity of pigs improved – the content of α1 and α2 globulins decreased by 4.1% and 7.4%, respectively. Also, in the experimental group, the concentration of albumins increased by 7.5%, which probably indicates an improvement in the functional state of the liver. As a result of feeding with a complex probiotic supplement in the experimental group the albumin-globulin ratio improved, which increased by 35.0%. Thus, the introduction of a complex probiotic feed additive into the ration of pigs changed the structure of the influence of exogenous and endogenous factors on metabolism, had a positive effect on the protein profile of the blood, and caused an increase of average daily gains in experimental animals by 5.8% compared to the control group.

Investigation of the effect of bio-ap-irga dietary supplements on the composition and properties of yogurts

Today, consumers pay special attention to the quality of their products. They also expect a high level of innovation. Thus, the dairy sector's supply is increasingly focusing on the use of various additives with proven health benefits. The modern market of functional products consists of 65% dairy products. The basis of the technology of functional food products is the modification of traditional products, which ensure an increase in the content of useful ingredients in them to a level correlated with the physiological norms of consumption. Many scientific studies from different regions of the world are engaged in research, and their goal is to identify herbal supplements that have a beneficial effect on the human body. A specific feature of plant raw materials is the ability to synthesize a large number of various chemical compounds of various natures that have physiological activity. This article presents the results of studies on the effect of a biologically active additive obtained by using whey protein concentrate, saskatoon berries and mountain ash. The results of the physico-chemical, organoleptic parameters of yogurt obtained using the developed dietary supplement are presented. It was found that the content of proteins, vitamin C, and polyphenols in the finished yoghurts significantly increased. An increase in antioxidant properties was noted. The data obtained indicate the biological value of the developed yogurt was enriched with BIO-AP-IRGA dietary supplement.

Study of the retina macular region microvascular bed parameters in healthy people of the older age group according to optical coherence tomography with angiography function

BACKGROUND: To date, there is insufficient data on normal blood flow indicators in the retinal choroid plexuses in healthy people of the older age group. AIM: To create a normative basis for average indicators of vessel density in the superficial and deep plexuses of the retina, parameters of the foveal avascular zone and retinal thickness in the macular region in healthy people of the older age group according to optical coherence tomography with angiography. MATERIAL AND METHODS: The retrospective study included 73 people (146 eyes) aged from 58 to 79 years with preserved visual functions and somatic parameters corresponding to the age norm. Optical coherence tomography with angiography function analyzed the density of vessels in the superficial and deep retinal plexuses, the area and perimeter of the foveal avascular zone, the density of vessels in the area up to 300 μm from the foveal avascular zone, and the thickness of the retina. Statistical processing of the data was performed in MS Office Excel 2016. The data was presented in the format M±σ, where M was the average value of the indicator, σ was the standard deviation. RESULTS: In the study group, the overall average retinal thickness in the macular area was 279.9±14.8 µm. The overall average density of vessels of the superficial retinal plexus in the macular zone of the retina was 48.7±2.5%. The overall average density of vessels in the deep retinal plexus in the macular area was 51.9±3.7%. The area of the foveal avascular zone in healthy people was on average 0.322±0.123 mm2; the density of vessels within 300 µm from the foveal avascular zone was 53.8±3.3%. CONCLUSION: The age-related physiological norm for blood flow indicators in the retinal choroid plexuses in healthy people of the older age group has been established.

RECOVERY OF FOOTBALL PLAYERS BODIES AFTER INTENSIVE PHYSICAL ACTIVITY

The article contains recommendations for providing food in accordance with physiological norms for the process of restoring the energy used for the recovery of the athlete's body and adjusting the diet depending on the content of necessary nutrients (proteins, minerals, water, mineral products) in the provision of plastic substances.

An Attenuated Vaccine Virus of the Neethling Lineage Protects Cattle against the Virulent Recombinant Vaccine-like Isolate of the Lumpy Skin Disease Virus Belonging to the Currently Established Cluster 2.5.

Lumpy skin disease (LSD) is an emerging transboundary and highly infectious viral disease mainly affecting cattle. The fact that it was initially confined to Africa and then spread beyond its geographical range to other regions, including the Middle East, Turkey, Europe, the Balkans, Russia and Asia, is an indication of the underestimation and neglect of this disease. Vaccination is considered the most effective way to control the spread of LSDV, when combined with other control measures. LSD is now on the rise in Southeast Asia, where the circulating virus belongs to recombinant lineage 2.5. In this study, we evaluated the efficacy of an attenuated LSDV strain belonging to the Neethling cluster 1.1 by challenge with a virulent recombinant vaccine-like LSDV isolate "Mongolia/2021" belonging to cluster 2.5. Some of the vaccinated animals showed an increase in body temperature of 1-1.5 °C above the physiological norm, without clinical signs, local reactions, vaccine-induced viremia or generalization, demonstrating the efficacy and safety of the vaccine strain against a recombinant strain. Furthermore, all the vaccinated animals showed strong immune responses, indicating a high level of immunogenicity. However, the control group challenged with "Mongolia/2021" LSD showed moderate to severe clinical signs seen in an outbreak, with high levels of virus shedding in blood samples and nasal swabs. Overall, the results of the present study demonstrate that the attenuated LSDV Neethling strain vaccine has a promising protective phenotype against the circulating strains, suggesting its potential as an effective tool for the containment and control of LSD in affected countries from Southeast Asia.