Physalis Ixocarpa Brot Research Articles

Adventitious shoot formation and plant regeneration from tissues of tomatillo (Physalis ixocarpa Brot.)

Cultured hypocotyl explants of tomatillo (Physalis ixocarpa Brot.), were evaluated with regard to their morphogenic responses to combinations of benzyladenine (BA, 0–5 μM) with either naphthaleneacetic acid (NAA, 0–50 μM) or 2,4-dichlorophenoxyacetic acid (2,4-D, 0–50 μM). The induction of shoots or roots was dependent on the cytokinin/auxin combination.

Induced mutation in tomatillo (Physalis ixocarpa Brot.)

To induce mutation in tomatillo (Physalis ixocarpa Brot.) commonly grown for its fruit value, seeds were treated with three alkylating agents (dimethyl sulphate, diethyl sulphate and methyl ethane sulphonate) and gamma rays. Seven viable and five chlorophyll mutant types were screened both from M1 and M2 generations. The isolated mutant lines are described and evaluated with reference to their beneficial value.

In vitro haploid plantlet induction in Physalis ixocarpa brot. through microspore embryogenesis

Anthers of Physalis ixocarpa Brot. exised from 2-3 mm long flower buds were treated for 2 d at 3°C, and were cultured on the basal medium of NN (1969), supplemented with plant hormones. They formed embryoids from microspores within 6 weeks. Upon transfer to a regeneration medium, embryoids grew into functional plants with the haploid number of chromosomes (n=12).