Photosynthetic Index Research Articles

Variations in Growth and Photosynthetic Traits of Polyploid Poplar Hybrids and Clones in Northeast China.

To evaluate differences among 19 different ploidy hybrid poplar clones grown in northeast China, 21 traits related to growth traits and photosynthetic characteristics were detected and analyzed. Abundant phenotypic variations exist among and within populations, and these variations are the basis of forest tree genetic improvements. In this research, variance analysis showed that the traits except the net photosynthesis rate among the different ploidies and all the other traits exhibited significant differences among the ploidies or clones (p < 0.01). Estimation of phenotypic coefficients of variation, genotypic coefficients of variation, and repeatability is important for selecting superior materials. The larger the value, the greater the potential for material selection improvement. The repeatability of the different traits ranged from 0.88 to 0.99. The phenotypic and genotypic coefficients of variation of all the investigated traits ranged from 6.88% to 57.40% and from 4.85% to 42.89%, respectively. Correlation analysis showed that there were significant positive correlations between tree height, diameter, and volume. Transpiration rate, intercellular carbon dioxide concentration, and stomatal conductance were significantly positively correlated with each other but negatively correlated with instantaneous water use efficiency. Growth traits were weakly correlated with photosynthetic indexes. The rank correlation coefficient showed that most of the growth indicators reached a significant correlation level among different years (0.40-0.98), except 1-year-old tree height with 4-year-old tree height and 1-year-old ground diameter with 3-year-old tree height, which indicated the potential possibility for early selection of elite clones. Principal analysis results showed that the contribution rate of the first principal component was 46.606%, and 2-year-old tree height, 2-year-old ground diameter, 3-year-old tree height, 3-year-old ground diameter, 3-year-old diameter at breast height, 3-year-old volume, 4-year-old tree height, 4-year-old ground diameter, 4-year-old diameter at breast height, and 4-year-old volume showed higher vector values than other traits. With the method of multiple-trait comprehensive evaluation to evaluate clones, SX3.1, SY3.1, and XY4.2 were selected as elite clones, and the genetic gains of height, basal diameter, diameter at breast height, and volume of selected clones ranged from 12.85% to 64.87% in the fourth growth year. The results showed fundamental information for selecting superior poplar clones, which might provide new materials for the regeneration and improvement of forests in Northeast China.

Influence of Different Intensities of Tillage on Physiological Characteristics and Productivity of Crop-Rotation Plants.

The aim of this study was to evaluate the effect of different intensities of tillage on the physiological characteristics and productivity of plants in crop rotation. Five tillage practices (DP-deep ploughing (22-24 cm); SP-shallow ploughing (16-18 cm); SH-shallow harrowing (8-10 cm); DH-deep harrowing (14-16 cm); and DD-direct drilling) were investigated in a long-term experiment in Dotnuva. The crop rotation was as follows: winter oilseed rape → spring wheat → spring barley → field pea → winter wheat. The simplification of conventional tillage negatively affected the photosynthetic indices of the majority of the crop rotation plants. The most favorable conditions for the photosynthetic processes in the plants were identified in the deep-ploughing treatment. The photochemical activity was negatively influenced and leaf senescence was accelerated under direct drilling. Direct drilling significantly decreased the grain yield of winter oilseed rape, spring wheat, and spring barley by 10.5%, 12.8%, and 17.2%, respectively, compared to deep ploughing. The grain yield of winter wheat was similar under deep ploughing and direct drilling; conversely, under shallow ploughing, shallow harrowing, and deep harrowing, the yield tended to decrease compared to deep ploughing.

Transcriptome and hormones analysis provide insights into elongated fruit somaclonal mutant in ‘Akihime’ strawberry

Fruit shape is an important agronomic trait. However, the regulatory mechanism of strawberry fruit shape remains largely unknown. In this study, we firstly compared cultivated strawberry ‘Akihime’ with its elongated fruit somaclonal mutant (finger strawberry). The plant height, crown diameter, leaf length and some photosynthetic indexes were no significant changes between ‘Akihime’ and finger strawberry, while the fruit shape of them had obvious differences. Histological observation showed that fruit shape changes mainly resulted from the change of cell area, especially cell length, at small green fruit stage of ‘Akihime’ and finger strawberry. Next, the endogenous hormone contents of small green fruit of ‘Akihime’ and finger strawberry were investigated by HPLC-MS/MS. We found that the auxin content in the finger strawberry dramatically increased compared with ‘Akihime’, while the content of gibberellin and brassinosteroids had no significant difference between ‘Akihime’ and finger strawberry. Finally, small green fruits of ‘Akihime’ and finger strawberry were used for identifying key genes responsible for the elongated fruit phenotype through RNA-seq. A total of 674 differentially expressed genes (DEGs) were identified, including 248 up-regulated genes and 426 down-regulated genes. GO and KEGG enrichment analysis showed the DEGs were mainly enriched in DNA replication, cell cycle, plant hormone synthesis and signal transduction. Interestingly, auxin-related genes such as FaIAA33, FaSAUR76, FaSAUR34, FaARF and some transcription factors such as FaMYBs, FabHLHs, FaNACs were differentially expressed in ‘Akihime’ and finger strawberry, which may play a crucial role in the formation of elongated fruit mutant phenotype. These results will provide some insights into the regulatory mechanism of strawberry fruit shape and are helpful for appearance quality improvement of strawberry.

Effects of Nitrogen and Phosphorus Addition on Agronomic Characters, Photosynthetic Performance and Anatomical Structure of Alfalfa in Northern Xinjiang, China

The productivity of alfalfa is associated with a large amount of nitrogen (N) and phosphorus (P); the addition of exogenous N and P fertilizers can fully exploit the growth potential of alfalfa. However, there is uncertainty about the relationship between changes in alfalfa productivity and photosynthetic physiology and anatomy. We conducted field fertilization experiments on alfalfa in the second and third years under drip irrigation, as well as measurement of the photosynthetic physiology, anatomical structure and agronomic traits of alfalfa at different levels of N (0, 120 kg·ha−1) and different levels of P2O5 (0, 50, 100 and 150 kg·ha−1). The results showed that the dry matter yield (DMY), crude protein (CP), net photosynthetic rate (Pn) and specific leaf weight (SLW) were increased by 2.10~11.82%, 4.95~11.93%, 4.71~7.59% and 2.02~7.12% in the N application treatment compared with the non-N application treatment, while the DMY, CP, Pn and SLW were increased by 3.19~17.46%, 1.99~8.42%, 6.15~24.95% and 2.16~11.90% in the P application treatment compared with the non-P application treatment. N and P increase the thickness of the spongy tissue (ST) of alfalfa, which will facilitate the entry and exit of gas and water, and will further affect the photosynthetic indexes, such as stomatal conductance (Gs) and transpiration rate (Tr), of alfalfa leaves. Increased palisade tissue (PT) thickness will also enhance the adaptability of plant leaves to strong sunlight, thereby increasing the maximum net photosynthetic rate (Pmax) and light saturation point (LSP). Fertilization treatment showed the highest utilization efficiency for low light and better adaptation to strong light, but the Rd decreased. The comprehensive scores of principal component analysis for anatomical structure, photosynthetic performance and agronomic traits were N1P2 &gt; N0P2 &gt; N1P3 &gt; N1P1 &gt; N0P3 &gt; N0P1 &gt; N1P0 &gt; N0P0. Therefore, the application of N and P fertilizers contributed to the adaptive changes in alfalfa leaf anatomy and the improvement of photosynthetic capacity, which were beneficial to the improvement of alfalfa dry matter yield, growth traits and nutritional quality, with the most obvious improvement effect obtained with the application of 120 kg·ha−1 of N and 100 kg·ha−1 of P2O5.

Effects of Different Irrigation Modes on the Growth, Physiology, Farmland Microclimate Characteristics, and Yield of Cotton in an Oasis

In order to determine the effects of different water-saving irrigation techniques on physiological growth, farmland microclimate, and yield of cotton (Gossypium Spp.), a two-year field experiment was carried out in an oasis area of Northwest China, and three irrigation methods were tested, including on-film irrigation (T1), under-film drip irrigation (T2), and automatic irrigation (T3). The results showed that the relative humidity, plant height, leaf area, stem thick, and photosynthetic index with the T3 treatment were significantly higher than those with T2 and T1. The air and soil temperature with T3 (except seedling stage) were considerably lower than those with T2 and T1. According to the fitting and statistical analysis of each index and yield, except for air and soil temperature, the other indices were positively correlated with yield. Based on the analysis of each index, the T3 treatment had the most significant regulatory effect on cotton’s physiological growth and farmland microclimate. Compared with T1, the irrigation amounts of T2 and T3 decreased by 16.43% and 25.90%, but the yield increased by 38.96% and 46.28%, respectively. The automatic irrigation strategy showed significant advantages in water saving and yield increase, which could provide some reference for cotton drip irrigation in similarly arid areas.

Developmental and phytochemical changes in pot marigold (Calendula officinalis L.) using exogenous application of polyamines

Polyamines (PAs) are natural active compounds having more than two amino groups that play important roles in many physiological and developmental processes in plants. The purpose of this research was to see how foliar polyamine spray affected growth and photosynthetic indices, as well as secondary metabolites and antioxidant activity of the aqueous and methanolic extracts of pot marigold (Calendula officinalis L.). The experiment lasted for three months and was arranged in a randomized complete design with four replications. Three separate concentrations (0.5, 1 and 2.5 mM) of spermine (SPM), spermidine (SPD), and putrescine (PUT) were sprayed at four/five fully expanded leaf stage and some physiochemical attributes were evaluated. The treatments caused a significant increase in morphological and photosynthetic parameters and total oil. There were also significant variations in total phenolic and flavonoid content. Compared to other polyamines, 1 mM SPD foliar spraying showed the greatest effect. Furthermore, the highest antioxidant capacity (DPPH* scavenging assay, ferric reducing antioxidant power (FRAP), Trolox equivalent antioxidant capacity (TEAC) and β-carotene bleaching activity) was observed in the 1 mM SPD treatment. The results showed that the calendula essential oils (EOs) were rich in sesquiterpenes hydrocarbons (55.92–95.94%), with c-Cadinene and d-Cadinene as the major sesquiterpenes in the EOs. Also, the flowers were rich sources of carotenoids (lutein, flavoxanthin and luteoxanthin) following polyamines application. Hence, it can be inferred that polyamines specially spermidine would find a wide range of application in pharmaceutical industries due to its impact on antioxidant properties of phenolic and flavonoid compounds.

Tetraploidy in Citrus wilsonii Enhances Drought Tolerance via Synergistic Regulation of Photosynthesis, Phosphorylation, and Hormonal Changes.

Polyploidy varieties have been reported to exhibit higher stress tolerance relative to their diploid relatives, however, the underlying molecular and physiological mechanisms remain poorly understood. In this study, a batch of autotetraploid Citrus wilsonii were identified from a natural seedling population, and these tetraploid seedlings exhibited greater tolerance to drought stress than their diploids siblings. A global transcriptome analysis revealed that a large number of genes involved in photosynthesis response were enriched in tetraploids under drought stress, which was consistent with the changes in photosynthetic indices including Pn, gs, Tr, Ci, and chlorophyll contents. Compared with diploids, phosphorylation was also modified in the tetraploids after drought stress, as detected through tandem mass tag (TMT)-labeled proteomics. Additionally, tetraploids prioritized the regulation of plant hormone signal transduction at the transcriptional level after drought stress, which was also demonstrated by increased levels of IAA, ABA, and SA and reduced levels of GA3 and JA. Collectively, our results confirmed that the synergistic regulation of photosynthesis response, phosphorylation modification and plant hormone signaling resulted in drought tolerance of autotetraploid C. wilsonii germplasm.

Interactive effect of soil moisture content and phosphorus fertilizer form on chickpea growth, photosynthesis, and nutrient uptake

Water shortage and soil nutrient depletion are considered the main factors limiting crops productivity in the Mediterranean region characterized by longer and frequent drought episodes. In this study, we investigated the interactive effects of P fertilizer form and soil moisture conditions on chickpea photosynthetic activity, water and nutrient uptake, and their consequent effects on biomass accumulation and nutrient use efficiency. Two P fertilizer formulas based on orthophosphates (Ortho-P) and polyphosphates (Poly-P) were evaluated under three irrigation regimes (I1: 75% of field capacity, I2: 50% FC and I3: 25% FC), simulating three probable scenarios of soil water content in the Mediterranean climate (adequate water supply, medium, and severe drought stress), and compared to an unfertilized treatment. The experiment was conducted in a spilt-plot design under a drip fertigation system. The results showed significant changes in chickpea phenotypic and physiological traits in response to different P and water supply regimes. Compared with the unfertilized treatment, the stomata density and conductance, chlorophyll content, photosynthesis efficiency, biomass accumulation, and plant nutrient uptake were significantly improved under P drip fertigation. The obtained results suggested that the P fertilizer form and irrigation regime providing chickpea plants with enough P and water, at the early growth stage, increased the stomatal density and conductance, which significantly improved the photosynthetic performance index (PIABS) and P use efficiency (PUE), and consequently biomass accumulation and nutrient uptake. The significant correlations established between leaf stomatal density, PIABS, and PUE supported the above hypothesis. We concluded that the Poly-P fertilizers applied in well-watered conditions (I1) performed the best in terms of chickpea growth improvement, nutrient uptake and use efficiency. However, their effectiveness was greatly reduced under water stress conditions, unlike the Ortho-P form which kept stable positive effects on the studied parameters.

Influence of reduced N-fertilizer application on foliar chemicals and functional qualities of tea plants under Toxoptera\xa0aurantii infestation

BackgroundThe tea aphid, Toxoptera aurantii (Boyer de Fonscolombe) is a polyphagous pest predominant in tea orchards and has become the most pernicious pest deteriorating tea quality. Nitrogen (N) is essential to plant growth improvement, and it can significantly impact plant defensive ability against aphid infestation. This study was designed to quantify the influence of reduced N-fertilizer application on foliar chemicals and functional quality parameters of tea plants against the infestation of T. aurantii. In this study, the tea seedlings (cv. Longjing43) were applied with normal level (NL) of N-fertilizer (240 kg N ha−1) along with reduced N-fertilizer levels (70%NL and 50%NL), and with and without T. aurantii infestation.ResultsThe results showed that N-fertilizer application significantly affected plant biomass and photosynthetic indexes, foliar soluble nutrients and polyphenols, tea catechins, caffeine, essential amino acids, volatile organic compounds of tea seedlings, and the population dynamics of T. aurantii. Compared with the normal N-fertilizer level, the reduced N-fertilizer application (70%NL and 50%NL) significantly decreased all the foliar functional quality components of tea seedlings without aphid infestation, while these components were increased in tea seedlings with aphid infestation. Moreover, the transcript expression levels of foliar functional genes (including CsTCS, CsTs1, and CsGT1) were significantly higher in the NL, and significantly lower in the 50%NL for tea seedlings without aphid infestation, while the transcript expression levels were significantly higher in 50%NL in aphid inoculated tea seedlings.ConclusionThe results demonstrated that the reduced N-fertilizer application could enhance foliar chemicals and functional quality parameters of tea plants especially with T. aurantii infestation, which can relieve soil nitrogen pressure and reduce pesticide use for control of tea aphid infestation in tea plantations.

Photosynthetic Properties and Structure of Leaves of Licorice Seedlings in Response to Drought Stress

Licorice (Glycyrrhiza uralensis Fisch.) is a perennial herb that has high drought resistance, and is widely used in food, pharmaceutical and cosmetics industries. Understanding the response mechanism of licorice leaves to drought stress can help to develop drought-tolerant medicinal crops. In this experiment, the changes of photosynthetic parameters and structure of licorice leaves under drought stress were investigated. After 10 days of treatment with different soil containing water of 80, 65, 50 and 35%, the photosynthetic indexes, leaf surface stomata, glands morphology, leaf structure and chloroplast structure of licorice seedlings were measured respectively. The results showed that under light stress (LS), licorice adapted to drought stress by adjusting stomatal number, morphology and gland density, while severe stress (SS) inhibited the electron transfer between photosystems, leading to inactivation of the oxygen emitting complex, destruction of chloroplast structure and insufficient CO2 supply, resulting in the decrease of photosynthesis. This paper explains the response mechanism of licorice leaves to drought stress, which provides a foundation for further exploring its mechanism and improving the yield.

Decoding the potential of a new Pseudomonas putida strain for inducing drought tolerance of tomato (Solanum lycopersicum) plants through seed biopriming

A total of 11 potential plant growth promoting rhizobacteria previously isolated from naturally stressed environments were evaluated for various traits of interest for a beneficial symbiosis with plants, including colonization ability, biofilm formation, motility, exopolysaccharide production and salt tolerance. The vast majority of the strains were found to possess multiple plant growth promoting traits. Nevertheless, the intensity varied among isolates, with those originated from tomato plants being more efficient colonizers. The strain SAESo11, genetically characterized as a Pseudomonas putida member was selected for further investigation of its potential to alleviate drought stress in tomato seedlings. Inoculation with SAESo11 mitigated the negative effects of drought stress as indicated by growth and photosynthetic indices. Furthermore, bacterial inoculation enhanced H2O2 content and malondialdehyde levels in colonized plants. Drought treatment did not further alter the oxidative status of these plants. Similarly, total phenolic content and antioxidant enzyme activity were induced in plant tissues in response to drought stress only at the absence of inoculum. These results indicated that inoculation with the selected strain imposed plants at a priming state, that enabled them to respond more robustly at the exposure to drought stress and efficiently attenuated the drought-induced injury. This state of plant alertness mediated by SAESo11 occurred at no cost to growth, highlighting its role as a potential plant priming agent.

Kaolin Film Increases Gas Exchange Parameters of Coffee Seedlings During Transference From Nursery to Full Sunlight.

Increases in water use efficiency (WUE) and the reduction of negative impacts of high temperatures associated with high solar radiation are being achieved with the application of fine particle film of calcined and purified kaolin (KF) on the leaves and fruits of various plant species. KF was applied on young Coffea arabica and Coffea canephora plants before their transition from nursery to full sunlight during autumn and summer. The effects of KF were evaluated through the responses of leaf temperature (Tleaf), net CO2 assimilation rate (A), stomatal conductance (gs), transpiration (E), WUE, crop water stress index (CWSI), index of relative stomatal conductance (Ig), initial fluorescence (F0), and photosynthetic index (PI) in the first 2–3 weeks after the plant transitions to the full sun. All measurements were performed at midday. In Coffea plants, KF decreased the Tleaf up to 6.7°C/5.6°C and reduced the CWSI. The plants that were not protected with KF showed lower A, gs, E, and Ig than those protected with KF. C. canephora plants protected with KF achieved higher WUE compared with those not protected by 11.23% in autumn and 95.58% in summer. In both Coffea sp., KF application reduced F0, indicating reduced physical dissociation of the PSII reaction centers from the light-harvesting system, which was supported with increased PI. The use of KF can be recommended as a management strategy in the transition of Coffea seedlings from the nursery shade to the full sunlight, to protect leaves against the excessive solar radiation and high temperatures, especially in C. canephora during the summer.

Short-term effects of combined freeze–thaw and saline–alkali stresses on the physiological response in highland barley (Hordeum vulgare).

Highland barley (Hordeum vulgare L.), as the dominant crop on the Qinghai-Tibetan Plateau, is a typical representative of plants adapted to extreme environmental conditions. However, the harsh environment, severe salinisation and frequent freezing and thawing in the Qinghai-Tibetan Plateau are main limiting factor for crop growth in this region. The physiological response of highland barley to salinisation and freeze-thaw stresses was studied in this paper. Under the combined stresses of 60mmol/LNaCl·60mmol/LNaHCO3 and freeze-thaw cycles (10, -5, and 10°C), the changes in the relative moisture content, relative electrical conductivity, soluble protein, malondialdehyde (MDA) and photosynthetic indices Pn and E in seedling leaves of eight groups of treatments (CK, S, A, S-A, CK (FT), S (FT), A (FT), and S-A (FT)) were analysed. Results showed that a single stress did not cause a change in the MDA content. All of the combined stresses in S-A, CK (FT), S (FT), A (FT), and S-A (FT) treatments increased the MDA content of barley seedlings, and the MDA content of S-A (FT) reached 28.438 at T2 (-5°C) μmol/g. During the freeze-thaw cycle, the cell membrane of seedlings was damaged more seriously by alkali stress, which showed a significant increase in relative conductivity. The relative moisture content value of seedlings was more than 100% because the seedlings could absorb more moisture due to mechanical injury. The protein content of osmoregulatory substances in highland barley seedlings increased with increasing stress, indicating resistance to stress. Moreover, the effect of freeze-thaw stress on photosynthesis was more significant. The changes in indices proved that an appropriate amount of salt stress could improve the resistance of the plant cell membrane. Alkali stress had a significant effect on the growth of highland barley seedlings. Freezing and thawing can aggravate the damage of saline-alkali stress to highland barley seedlings, resulting in changes in the biological membrane permeability and photosynthesis of seedlings. The fluctuation of osmoregulation substance content confirmed that highland barley seedlings had a certain degree of stress resistance. Freeze-thaw cycles will aggravate the damage of land salinisation to highland barley seedlings. To better reduce the impact and loss of land salinisation and freeze-thaw disasters on agriculture in the Qinghai-Tibetan Plateau, priority should be given to solving freeze-thaw stress in the process of grain production.

Biochemical and Physiological Responses of Harmful Karenia mikimotoi to Algicidal Bacterium Paracoccus homiensis O-4.

Harmful algal blooms caused by Karenia mikimotoi frequently occur worldwide and severely threaten the marine environment. In this study, the biochemical and physiological responses of K. mikimotoi to the algicidal bacterium Paracoccus homiensis O-4 were investigated, and the effects on the levels of reactive oxygen species (ROS), malondialdehyde content, multiple antioxidant systems and metabolites, photosynthetic pigments, and photosynthetic index were examined. The cell-free supernatant in strain O-4 significantly inhibited K. mikimotoi cell growth. The bacterium caused the K. mikimotoi cells to activate their antioxidant defenses to mitigate ROS, and this effect was accompanied by the upregulation of intracellular antioxidant enzymes and non-enzyme systems. However, the overproduction of ROS induced lipid peroxidation and oxidative damage within K. mikimotoi cells, ultimately leading to algal death. In addition, the photosynthetic efficiency of the algal cells was significantly inhibited by O-4 and was accompanied by a reduction in photosynthetic pigments. This study indicates that O-4 inhibits K. mikimotoi through excessive oxidative stress and impaired photosynthesis. This research into the biochemical and physiological responses of K. mikimotoi to algicidal bacteria provides insights into the prophylaxis and control of harmful algal blooms via interactions between harmful algae and algicidal bacteria.

The impact of competitive stress on photosynthetic indices in apple tree leaves

The impact of competitive stress on photosynthetic indices in apple tree leaves

Different responses of two Chinese cabbage (Brassica rapa L. ssp. pekinensis) cultivars in photosynthetic characteristics and chloroplast ultrastructure to salt and alkali stress.

Salt and alkali stress affected the photosynthetic characteristics of Chinese cabbages. A salt-tolerant cultivar maintained its tolerance by ensuring the high ability of photosynthesis. The synthesis of organic acids and carbohydrates in leaves played important roles in improving the photosynthetic capacity of alkali-tolerant plants. Soil salinization has become an increasingly serious ecological problem, which limits the quality and yield of crops. As an important economic vegetable in winter, however, little is known about the response of Chinese cabbage to salt, alkali and salt-alkali stress in photosynthetic characteristics and chloroplast ultrastructure. Thus, two Chinese cabbage cultivars, 'Qinghua' (salt-tolerant-alkali-sensitive) and 'Biyu' (salt-sensitive-alkali-tolerant) were investigated under stresses to clarify the similarities and differences between salt tolerance and alkali tolerance pathways in Chinese cabbage. We found that the root of Qinghua, the leaf ultrastructure and net photosynthetic rate (Pn), stomatal conductance (Gs), water use efficiency (WUE), maximum photochemical quantum yield of PSII (Fv/Fm) and nonphotochemical quenching (NPQ) were not affected by salt stress. However, Biyu was seriously affected under salt stress. Its growth indexes decreased by between 60 and 30% compared with the control and the photosynthetic indexes were also seriously affected under salt stress. This indicated that the salt-tolerant cultivar Qinghua improved the photosynthetic fluorescence ability to promote the synthesis of organic matter resulting in salt tolerance. In contrast, under alkali treatment, the root of Biyu was affected by alkali stress, but could still maintain good growth, and root and leaf structure were not seriously affected and could maintain the normal operations. Biyu improved its tolerance by improving the water use efficiency, regulating the synthesis of organic acids and carbohydrates, ensuring the synthesis of organic matter and ensured the normal growth of the plant.

Amelioration of AsV toxicity by concurrent application of ZnO-NPs and Se-NPs is associated with differential regulation of photosynthetic indexes, antioxidant pool and osmolytes content in soybean seedling

Arsenic is a significant food safety and environmental concern due to its mutagenic and carcinogenic effect on living organism. Soybean (Glycine max [L.] Merrill) is a global staple crop grown intensively in arsenic-contaminated regions of the world (e.g., Southern Province of China). Therefore, the objective of this study was to investigate whether Se-NPs and/or ZnO-NPs could be used as an eco-friendly and efficient amendment to reduce arsenic uptake and toxicity in soybean. Ten-days-old seedling, grown in vermiculite, were transferred to hydroponic media and further grown till V2 growth stage appeared. AsV (25 μM Na2HAsO4) stressed plants were treated with ZnONP (25 μM ZnO) and SeNP (25 μM Se) separately and in combination, which were grown for another 10 d. The result demonstrated that arsenic-treated soybean plants displayed a reduction in photosynthetic efficiency, increased proline and glycine betaine accumulation in tissues, and altered antioxidant activity compared to an untreated control. The application of zinc oxide and selenium nanoparticles, both independently and in tandem, reduced arsenic stress in root and shoot tissues and rescued plant health. This was reflected through increased levels of reduced glutathione content, ascorbic acid, and various photosynthesis- and antioxidant-relevant enzymes. In addition, nanoparticle-treated soybean plants displayed higher expression of defense- and detoxification-related genes compared to controls. Cellular toxicants (i.e., oxidized glutathione, reactive oxygen species, and malondialdehyde) were reduced upon nanoparticle treatment. These data collectively suggest that selenium and zinc oxide nanoparticles may be a solution to ameliorate arsenic toxicity in agricultural soils and crop plants.

Effects of Epichloësinensis Endophyte and Host Ecotype on Physiology of Festuca sinensis under Different Soil Moisture Conditions.

This study explored the effects of the Epichloë sinensis endophyte on growth, photosynthesis, ionic content (K+ and Ca2+), phytohormones (abscisic acid—ABA, cytokinin—CTK, indolE−3-acetic acid—IAA, and gibberellin—GA), and elements—C, N, P (in the shoot and root) in two ecotypes of Festuca sinensis (ecotypes 111 and 141) under different soil water conditions (35% and 65% relative saturation moisture content (RSMC)). The results showed that 35% RSMC inhibited the plants’ growth, and compared with 65% RSMC, there was a significant (p < 0.05) decrease in the growth and photosynthesis indices, the contents of CTK and GA, Ca2+ concentration, and the contents of C, N, and P (in both the aboveground and underground parts) under 35% RSMC. E. sinensis had beneficial effects on host growth and stress tolerance. Under both 35% and 65% RSMC, the presence of E. sinensis significantly (p < 0.05) increased host plant height, tiller number, root length, root volume, shoot dry weight, chlorophyll content, and the rate of photosynthesis of both ecotypes. Furthermore, the shoot C, N, and P contents in plants infected with E. sinensis (E+) from the two ecotypes, under both conditions of RSMC, were significantly (p < 0.05) higher than those in corresponding plants that were not infected with E. sinensis (E−). Under 35% RSMC, the contents of ABA, K+, Ca2+, and root P contents in E+ plants were significantly (p < 0.05) higher than those in corresponding E− plants in both ecotypes. However, under 65% RSMC, root C, N, and P contents in E+ plants of ecotype 111 and 141 were significantly (p < 0.05) higher than those in corresponding E− plants. In addition, the host ecotype also had effects on host growth and stress tolerance; the growth and photosynthetic indices of ecotype 141 were significantly (p < 0.05) higher than those of ecotype 111 under 35% RSMC, which suggested that ecotype 141 is more competitive than ecotype 111 under water deficiency conditions. These findings suggest that the endophyte improved the host plant resistance to water deficiency by maintaining the growth of the plant, improving photosynthesis, accumulating K+ and Ca2+, promoting nutrient absorption, and adjusting the metabolism of plant hormones.

Effects of Application Methods of Boron on Tomato Growth, Fruit Quality and Flavor

The effect of application methods with different boron levels on the growth, fruit quality and flavor of tomato (Solanum lycopersicum L., cv. ‘Jinpeng No.1’) were investigated under greenhouse conditions. Seven treatments used included two application methods (leaf and root application) with four boron levels (0, 1.9, 3.8 and 5.7 mg∙L−1 H3BO3). Experimental outcomes revealed that both application methods significantly increased net photosynthetic rate and chlorophyll content, and stabilized leaf structure of tomato. Leaf spray of 1.9 mg∙L−1 H3BO3 was more effective at improving plant growth and photosynthetic indices in tomato compared to other treatments. Additionally, root application of 3.8 mg∙L−1 H3BO3 resulted in better comprehensive attributes of fruit quality and flavor than other treatments in terms of amounts of lycopene, β-carotene, soluble protein, the sugar/acid ratio and characteristic aromatic compounds in fruit. The appropriate application of boron can effectively improve the growth and development of tomato, and change the quality and flavor of fruit, two application methods with four boron levels had different effects on tomato.

The Effect of Mineral-Based Mixtures Containing Coal Fly Ash and Sewage Sludge on Chlorophyll Fluorescence and Selected Morphological Parameters of Deciduous and Coniferous Trees

Coal fly ash (CFA), which is generated in huge quantities in coal-fired power plants, is a problem worldwide. Mixtures with ash and sewage sludge alter morphological and biochemical characteristics of plants. In this experiment, the response of pine, spruce, beech and alder growing for four years to mineral mixtures based on coal fly ash and high salinity sewage sludge (SS) was studied. The four-year experiment determined the chlorophyll a fluorescence of the tested plants, their height and yield, the salinity level of the tested mixtures and their phytotoxicity. Mixtures of coal ash with sewage sludge proved to be more beneficial to plants than their separate application. After four years, among the studied species, the highest increase in height and biomass was recorded for European alder and Scots pine. These species were also characterized by high photosynthetic indices. Mixtures containing 29% SS created optimal conditions for the development of the studied tree species. Grey alder and ponderosa pine can be recommended for reclamation of degraded areas where CFA and SS mixtures are used.