Menstruation Phase Research Articles

A study of pain perception by cold pressure test in young females during the different phases of menstrual cycle

Background: Menstruation is a cyclic physiological phenomenon showing fluctuations of various Gonadal hormones. Gonadal hormones (estrogen and progesterone) influences pain sensitivity, the former strongly influences nociceptive actions; whereas the latter prevents neuropathic pain some previous study has shown changes in pain sensitivity during menstruation. However, there is only little evidence present on effect of Gonadal hormones on experimental pain Sensitivity. Aims and Objectives: The aim of the present study is to differentiate in Sensation of pain stimulus in terms of - pain threshold, pain tolerance, pain intensity, pain unpleasantness in females with normal menstruation during different phases through using cold presser test. Materials and Methods: A total of 55 normal healthy females were enrolled in the study as per the eligibility criteria. Cold presser test was used as a pain stimulus source. The participants were instructed to hold their least dominant hand in the water bath as long as possible and were requested to inform the first sensation of pain which denoted the participants’ pain threshold. Time from pain threshold to the point where participants could no longer cope with pain and indicate stop, was recorded as pain tolerance. At this point, participants were directed to note pain intensity and unpleasantness on the visual analogue scale. Results: The results showed that high significant pain intensity, pain unpleasantness, and pain tolerance level were highly significant during follicular phase than luteal phase. Conclusion: In our study, we concluded pain perception difference across during different phase of menstruation in the form of high significant pain intensity, pain unpleasantness, and pain tolerance during follicular phase due to the hormonal fluctuation and the differences in autonomic nervous system reactivity these would be the underlying mechanism for these findings.

Relationship between menstruation phase and fatal self-directed violence among females of the reproductive age group

Relationship between menstruation phase and fatal self-directed violence among females of the reproductive age group

Cost of Menstrual Hygiene Practices in Gujarat, India

Menstrual cycles are an integral part of a nearly two-third of a woman’s life, and occur every month. Menstruation is a public health concern because irrespective of whether a woman can afford hygienic absorbents or not, she undergoes menstruation phase every month. Therefore, menstruation requires to be examined from public health policy perspective. Choice of menstrual products is not only subject to budget constraint but also to “other regarding preferences”, as can be explained by behavioural economics theories. This study therefore, seeks to get insights into the determinants of choice of menstrual product. The study attempts to devise a method of estimating the minimum yearly cost of menstrual hygiene and also provides an estimate for the same, which can be helpful for the policy prescription. The study is undertaken in Gujarat (India), from a cross-section of districts (developed, developing and tribal), and also a cross-section of socioeconomic status and age. A total of 1025 responses are collected and analysed to get insights into product preferences and resultant cost. The study finds that it is economical to make use of menstrual cups, however, cultural constrains might restrict its use.

PENGARUH PEMBERIAN JUS BIT TERFORTIFIKASI FESO4 INSTAN (JUS BEEFE) DALAM MENANGGULANGI ANEMIA ATLET REMAJA PUTRI

Prolonged physical exercise has the risk of increasing free radicals which will affect the breakdown of red blood cells and promote the risk of decreased hemoglobin. Adolescent female athletes have a risk of decreasing hemoglobin due to menstruation. This situation can get worse if the athletes have iron defi-ciency or anemia as the result of poor diet choice. Increased radicals and anemia affect the amount of oxygen carried to the tissues and the maximum volume of oxygen an individual can use to produce energy (VO2 max). Beets have benefits for athlete's performance and have been developed into an instant drink. The purpose of this study was to determine the effect of an instant FeSO4 fortified beet juice (BeeFe juice) in overcoming anemia on female adolescent athletes. This research using an experimental study with controlled trial pre-posttest design. Twenty-nine adolescent female athletes, aged 13-19 years, were divided into two groups. Fe tablet supplementation (containing Fe 60 mg) was given to 15 athletes in the control group and BeeFe juice (containing Fe 17 mg) was given to 14 athletes in the treatment group du-ring the luteal and menstruation phase (±14 days). Hemoglobin levels, Malondialdehyde (MDA) and maxi-mal oxygen volume (VO2 max) were examined before and after the intervention. Data were analyzed using paired t-test and independent t-test IBM SPSS version 22. This research has obtained Ethical Appro-val with number KE/FK/0633/EC/2018 from the Ethical Commission of FKKMK UGM. The results showed no significant difference between the group given Fe tablets and the group given BeeFe juice on hemo-globin levels (P>0.05), MDA levels (P>0.05), and VO2 max levels (P>0.05) in the menstrual and luteal phases of menstruation. It can be concluded that BeeFe juice has the same effectiveness as commercial Fe tablets. BeeFe juice can be an alternative food ingredient in iron supplementation.

Abstract 12055: Immediate Effect of Yoga Based Om-Meditation on Heart Rate Variability During Different Phases of Menstruation

Introduction: Stress may play a role in causing menstrual irregularities predisposing to increased risk for cardiovascular disease (CVD). Stress and cyclic variations in endogenous hormone levels during the menstrual cycle (MC) may affect heart rate variability (HRV), a measure of cardiac autonomic function (CAF) and mental stress. Strategies that reduce stress and improve HRV may prevent menstrual irregularities and thus CVD. We evaluated the effect of yoga-based OM-meditation on HRV during different phases of MC in young healthy females. Methods: A non-randomized controlled study was conducted on healthy young females (n=20) with normal menstrual cycle. Meditation group subjects (n=10) practiced Om-meditation while control group subjects (n=10) relaxed quietly for 10 minutes. Each subject received an intervention thrice during menstrual, proliferative and secretory phase. Data was collected before and immediately after every intervention. BMI, blood pressure (BP), heart rate (HR) and HRV were measured. Frequency domain method was used for an analysis of HRV. Results: The mean age of participants was 19.2±0.79 and 19.3±0.82 years; and mean period of MC was 31.1±2.76 and 30.3±2.4 days in meditation and control group respectively. There was no change in the baseline values of BMI, BP and HR between two groups and were within normal range. Between group analysis (ANCOVA) of HRV spectrum showed that Om-meditation had significantly decreased low frequency (LF) component of HRV (p<0.05) and LF/HF ratio (p<0.05); and increased high frequency (HF) component (p<0.05) of HRV during all the three phases of MC. Conclusion: These results suggest that practice of Om-meditation for 10 minutes can induce beneficial changes in HRV by reducing sympathetic activity and shifting autonomic balance towards parasympathetic dominance. Om-meditation may reduce mental stress and optimize CAF during different phases of MC. However, this needs an evaluation in a larger trial.

Bayesian Estimation of the Variation in Strength and Aerobic Physical Performances in Young Eumenorrheic Female College Students during a Menstrual Cycle.

Background: The purpose of the present investigation was to examine changes in strength and aerobic physical performances in young eumenorrheic female college students during the menstruation phase and different testing occasions within a menstrual cycle. Methods: A repeated measure experimental design used to investigate the variation in physical performance from different testing occasions compared to the menstruation phase. Twelve eumenorrhea female college students volunteered to participate in this study. The participants were 19.8 ± 0.8 (±SD) years old, with the body mass of 61.4 ± 11.6 kg, the height of 162.6 ± 5.1 cm, and BMI of 23.2 ± 3.8. All participants reported regular monthly menstrual cycles of 26–33 days, none of whom reported taking oral contraceptives in their entire life. None of the participants was an athlete, and their level of activity was limited to physical education classes and recreational activities. The menstrual cycles during the two cycles before testing had to be between 26 and 35 days to participate in this study. Second, there had to be no current or ongoing neuromuscular diseases or musculoskeletal injuries. Third, no one should be taking any dietary or performance-enhancing supplements that could have affected testing results during this study. The participants tested on one-repetition maximum (1RM) bench press, 1RM leg press, push-up to failure, leg press with 60% of 1RM to failure, and running 1600 m time trial. The participants were tested on four occasions based on the classical model of the menstrual cycle (i.e., 28 days; early follicular phase (menstruation phase) on day 2 (T1), late follicular phase on day 8 (T2), ovulation phase on day 14 (T3), and mid-luteal phase on day 21 (T4)). Data were analyzed using the Bayesian hierarchical model (Bayesian Estimation) with Markov Chain Monte Carlo simulation using the decision-theoretic properties of the high-density interval (HDI) + ROPE decision rule. Results: The Bayesian estimated difference from the four testing occasions neither showed that the most credible parameter values (95% HDI) were sufficiently away from the null value nor showed that the most credible parameter values are close to the null value (Rope odds ratio among all tests were spread in 12.7% < 0 < 87.3% with an effect size ranging between d = −0.01 and 0.44). Hence, no decision can be made as to whether strength and aerobic physical performances change during the menstruation phase compared to the other testing occasions within a menstrual cycle. Conclusions: It was noticed that different studies concluded different results, which make the research in menstrual cycle difficult. However, the results from this study and published studies suggest that future research should investigate and profile motivation and autonomic nervous system activity during the menstruation phase and examine the interaction effect of the three on performance compared to other testing occasions within a menstrual cycle.

Relationship between weight management and menstrual status in female athletes: a cross-sectional survey

ABSTRACT The aim of this study was to investigate the effects of weight management on menstrual status in female athletes. A total of 225 collegiate athletes and 27 para-athletes who belonged to teams affiliated with the Japanese Paralympic Committee were included in this cross-sectional survey. A self-reported questionnaire (containing information on the demographic characteristics, medical history, lifestyle habits, weight management, menstruation status, physical symptoms related to menstrual cycle, and the influence of physical symptoms experienced during the luteal phase of menstruation during training or competition.) was used to assess the results. In the collegiate athletes, the rate of regular menstrual cycle was significantly lower in those with weight loss than in those without (56.7% vs. 75.0%, P < .05). Furthermore, stress fractures were found significantly more often in those with weight loss than those without (36.1% vs. 20.3%, P < .05). In the para-athletes, 46.2% of experience in weight loss had irregular menstruations (P < .01), and all of them had physical symptoms that negatively affected their training or competition (P < .05). To prevent menstrual dysfunction related to energy deficiency in female athletes with weight management, menstrual status must be considered.

A study on influence of different phases of menstrual cycle on hematological parameters

Menstruation is the most common phenomenon observed in fertile women. Menstrual cycle (MC) is of 3 phases: proliferative phase, secretory phase and menstruation phase. It is controlled by endocrine system. Natural fluctuations in sex steroid hormones during MC causes changes in hematological parameters. The aim of the present study to assess the impact of different phases of MC on hematological parameters. The study was conducted in KIMS, Hubli, from 01st March 2011 to 31st March 2012. Women aged between 20-30 years with regular menstrual cycle of 27-30 days were included in the study. During each visit, the subjects’ blood was collected and analyzed using KX-21 SYSMEX for various hematological parameters. A total of 50 healthy young women were included in the study. On statistical comparing of hematological parameters, hematocrit, hemoglobin, neutrophil count and eosinophil count showed a significant difference while no statistically significant difference was observed in RBC, leucocyte count, lymphocyte count, monocyte count, erythrocyte sedimentation rate (ESR) and platelet count between different phases of MC. To conclude, the hematological parameters during the MC are highly dependent on the phasic changes in the immune response mechanism and sex steroid hormones.

The comparison of the effect of flaxseed oil and vitamin E on mastalgia and nodularity of breast fibrocystic: a randomized double-blind clinical trial

BackgroundFibrocystic changes are a common benign condition in women aged 20–50. The medical intervention aims to stop fibrocystic disease progress and relieve the breast’s pain and tenderness. In the long-term, reversing the fibrocystic changes is also desirable.MethodsIn this randomized double-blind clinical trial, the effect of flaxseed oil on the severity of pain and breast nodularity was investigated against vitamin E. This study was conducted on 100 women with mastalgia. The intervention group received Flaxseed oil pearls and the control group received vitamin E pearl 200 IU twice a day for 2 months. The duration and severity of breast pain were evaluated by Cardiff chart and VAS (Visual Analogue Scale). The nodularity was assessed by Lucknow-Cardiff scale at baseline, then the first and second months of intervention.ResultsAt baseline, there was no statistically significant difference between the two groups in characteristics. The breast pain improved in both groups during the first and second months of intervention (P-value within group< 0.001). However, the mean breast pain was not significantly different between the two groups at the end of the first and second month (P1= 0.54, P2= 0.73). Furthermore, the breast pain during four phases of the menstrual cycle showed no difference between vitamin E and flaxseed oil groups (menstruation phase= 0.76, follicular phase= 0.48, the first week of luteal phase= 0.86, the second week of luteal phase=0.30). The breast nodularity also decreased during the first and second months of intervention, yet no significant difference between the two groups was found (p= 0.9).ConclusionsThis study showed that flaxseed oil and vitamin E both could be effective in breast pain-relieving and decreasing nodularity with minimal side effects in contrast with the baseline. But there are no significant differences between these two agents. Larger scale prospective studies are needed to evaluate these effects in the long-term.Trial registrationIRCT201612243014N18, Registration date: 2017-10-15.

Association Between Trait Empathy and Resting Brain Activity in Women With Primary Dysmenorrhea During the Pain and Pain-Free Phases.

Empathy refers to the ability to understand someone else's emotions and fluctuates with the current state in healthy individuals. However, little is known about the neural network of empathy in clinical populations at different pain states. The current study aimed to examine the effects of long-term pain on empathy-related networks and whether empathy varied at different pain states by studying primary dysmenorrhea (PDM) patients. Multivariate partial least squares was employed in 46 PDM women and 46 healthy controls (HC) during periovulatory, luteal, and menstruation phases. We identified neural networks associated with different aspects of empathy in both groups. Part of the obtained empathy-related network in PDM exhibited a similar activity compared with HC, including the right anterior insula and other regions, whereas others have an opposite activity in PDM, including the inferior frontal gyrus and right inferior parietal lobule. These results indicated an abnormal regulation to empathy in PDM. Furthermore, there was no difference in empathy association patterns in PDM between the pain and pain-free states. This study suggested that long-term pain experience may lead to an abnormal function of the brain network for empathy processing that did not vary with the pain or pain-free state across the menstrual cycle.

The Frequency of Catamenial Epilepsy in Female Epileptic Patients of Reproductive Age Group Presented to the Tertiary Care Hospital.

Background and aimCatamenial epilepsy is the type of seizures during the reproductive phase of menstruation due to hormonal changes during the different phases of menstruation. This study aims to evaluate the frequency of epileptic seizures in women during the menstruation cycle and its management.Material and methodsThis study was conducted at the neurology department of Jinnah Postgraduate Medical Centre (JPMC), Karachi, Pakistan. The study's duration was six months, from the 22nd of January 2020 to the 22nd of July 2020. The sample size for catamenial epilepsy in female epileptic patients of reproductive age was 78%. After approval by the ethical committee of JPMC, data collection started. Data was collected and analyzed in the Statistical Package for the Social Sciences (SPSS, version 22; IBM Inc., Armonk, USA). Mean, and the standard deviation was calculated for age, duration of epilepsy, duration of antiepileptic, and antiepileptic drug. A Chi-square test was applied, and p≤0.05 was considered a statistically significant difference.ResultsA total of 184 female patients of reproductive age were selected for this study. The mean duration of epilepsy was 15.96 ± 8.85 months. The mean duration of antiepileptic drugs was 11.16 ± 7.53 months. In 73 patients (39.7%), EEG showed increased seizure activity during particular phases of the menstrual cycle. Catamenial epilepsy was seen in 73 patients (39.7%). The stratification according to age, duration of epilepsy, duration of antiepileptic drugs, the antiepileptic drug was done to observe the effect of these modifiers on catamenial epilepsy.ConclusionCatamenial epilepsy is relatively common epilepsy. The physician should evaluate patients when the seizures are refractory to the treatment. The females should manage a seizure diary, which will be beneficial in the management of epilepsy. In women with epilepsy, catamenial epilepsy should be considered in the diagnosis when the seizures are refractory to optimal treatment.

Effects of repeated menstrual pain on empathic neural responses in women with primary dysmenorrhea across the menstrual cycle.

Primary dysmenorrhea (PDM) is cyclic menstrual pain in the absence of pelvic anomalies, and it is thought to be a sex‐hormone related disorder. Existing study has focused on the effects of menstrual cramps on brain function and structure, ignoring the psychological changes associated with menstrual pain. Here we examined whether pain empathy in PDM differs from healthy controls (HC) using task‐based functional magnetic resonance imaging (fMRI). Fifty‐seven PDM women and 53 matched HC were recruited, and data were collected at the luteal and menstruation phases, respectively. During fMRI scans, participants viewed pictures displaying exposure to painful situations and pictures without any pain cues and assessed the level of pain experienced by the person in the picture. Regarding the main effect of the pain pictures, our results showed that compared to viewing neutral pictures, viewing pain pictures caused significantly higher activation in the anterior insula (AI), anterior cingulate cortex, and the left inferior parietal lobule; and only the right AI exhibited a significant interaction effect (group × picture). Post‐hoc analyses confirmed that, relative to neutral pictures, the right AI failed to be activated in PDM women viewing painsss pictures. Additionally, there was no significant interaction effect between the luteal and menstruation phases. It suggests that intermittent pain can lead to abnormal empathy in PDM women, which does not vary with the pain or pain‐free phase. Our study may deepen the understanding of the relationship between recurrent spontaneous pain and empathy in a clinical disorder characterized by cyclic episodes of pain.

Cortisol Awakening Response and Heart Rate Variability in the Menstrual Cycle of Sportswomen

ABSTRACT Purpose: This study aimed to examine the activities of heart rate variability (HRV) and cortisol secretion as markers of the autonomous nervous system (ANS) and the hypothalamic-pituitary-adrenal axis (HPA) during the menstrual cycle in female athletes. Method: Saliva samples of the participants (n = 28) were collected successively at 0, 15, 30, and 60 min after awakening to assess cortisol awakening response (CAR) during each of four phases of the menstrual cycle (the menstruation, ovulation, luteal, and premenstrual phases). Diurnal saliva samples were collected at noon, in the evening (17:00) and 22:00 for cortisol analysis. HRV was measured in the menstrual and premenstrual phases at rest (12:00–14:00). Results: There was no difference between CAR parameters in the menstrual phases. CAR parameters and diurnal cortisol levels were significantly correlated with each other throughout the menstrual cycle. Mean cortisol was a strong predictor of the area under the curve (AUC: S = 0.042; R-Sq = 98.4%). Time-dependent parameters of HRV (standard deviation of the N–N interval [SDNN; P = .049] and the root mean square of the successive R–R differences [rMSSD; P = .038]) were significantly higher in the menstrual phase. There were no correlations between CAR and HRV parameters. Conclusions: We suggest that the hypothalamic-pituitary-adrenal axis activity and the autonomic nervous system work in a separate manner that is not affected by the menstrual phases in sportswomen. The present study contributed to a better understanding of the relationship between the dynamics of cortisol release and the autonomic nervous system in different phases of the menstrual cycle in female athletes.

Serum Glucose Level According to Menstrual Cycle Phases in Young Students at the College of Science for Women

Objective: This study was carried out to present data on serum fasting glucose level in healthy young women with regular menstrual cycle who have moderate active state across their three different stages of the menstrual cycle (menstruation, proliferation and secretory) phases. Subjects and Method: A total of 50 non-diabetes women with normal regular menstrual cycle were involved in this study, the subjects ages ranged from 20-24 years, fasting venous blood (3ml) was collected at early morning during menstruation phase (days 1-5), proliferation phase (6-14 days) and the secretory phase (15- 28days) for each subject, serum glucose measurement were conducted by colorimetrictechnique. Results: The present study shows non-significant (p&lt;0.05) increase in the mean of glucose level during menstruation and proliferative phase (92.8±12.7, 92.6±10.2 mg/dl) respectively in the same subject comparison with secretory phase (90.48±8.3mg/dl) in the same subject. Conclusion: This study revealed that serum glucose level does not change along stages of normal and regular menstrual at rest or moderate active young women.

Gaps or links between hormonal therapy and schizophrenia? (Review).

Schizophrenia is considered the most severe and debilitating psychiatric disorder. During the 80's, first reports on abnormalities of the schizophrenic brain which could be objectively observed on MRI, CT scans and other imagistic techniques were published. This showed that schizophrenia is a disorder that goes beyond the functional aspect of the symptomatology. The ties between psychiatry and endocrinology are easily observed, even empirically, by any mental health practitioner, and mirrored by endocrinology specialists. Disorders related to menstruation phase of the menstrual cycle have a code in DSM-V, people expect women ‘to have mental disturbances’ during puberty, pregnancy, menopause and other periods of life known to cause a hormonal storm. Leaving aside those simple and common beliefs, any mental health specialist can observe the differences between men and women when it comes to psychopathology, and the differences between male and female patients when it comes to a severe disorder such as schizophrenia. Males present more severe symptoms; their evolution is worse and they tend to have more medico-legal issues. On the contrary, the current available treatments for schizophrenia tend to have some side effects easily observed by endocrinologists: from gynecomastia to breast asymmetry in women, hyperprolactinemia, weight gain and other metabolic disorders, the clinic shows us regularly what the science has already told us; that the impact of hormones on the developing brain, starting in utero and going on through life may hold the key to finding better treatments for debilitating disorders such as schizophrenia. This mini-review is focused on the role of estrogen in the evolution of schizophrenia and on reporting trials that showed how hormonal therapy (used mainly for breast cancer and osteoporosis) can improve the outcome of patients with schizophrenia.

683-P: Menstrual Cycle and Glycemic Differences during Prolonged, Fasting Exercise in Females with Type 1 Diabetes

Glycemic control may differ throughout the menstrual cycle, with increased incidence of hyperglycemia in the luteal phase. However, it is unclear if glycemic differences exist in the follicular vs. luteal phase during prolonged fasted exercise in T1D. The aim of this study was to determine if the early follicular vs. luteal phase impacts blood glucose levels during and post-exercise. Females with T1D (n = 7, age 28±6 years, A1c 6.9±0.6%) completed two 2-hr aerobic exercise sessions (∼45% VO2peak), during the luteal (days 18-24) and early-follicular phases (days 1-6) of menstruation. Carbohydrate (CHO) intake (0.3g/kg/hr) occurred every 30-min if glucose was between 72-180 mg/dL. No differences existed between the two phases for starting glucose, changes in glucose, CHO consumption and CHO oxidation during exercise (Table). Baseline glucagon was elevated in the luteal (28±25pg/mL) vs. follicular phase (13±12pg/mL; P&amp;lt;0.05). Following exercise, overnight time in hyperglycemia (&amp;gt;180 mg/dL) was greater in the luteal (59±44%) vs. follicular phase (21±30%; P&amp;lt;0.05). Overall, glycemic responses during prolonged fasting exercise are similar between luteal and follicular phases in women with T1D, as are CHO intake needs. However, after exercise in the luteal phase, glycemia tends to be elevated and appears to contribute to greater time in hyperglycemia. Disclosure S.M. McGaugh: None. D. Zaharieva: Speaker’s Bureau; Self; Ascensia Diabetes Care, Insulet Corporation, Medtronic. R. Pooni: None. N.C. D’Souza: None. J.E. Yardley: Research Support; Self; Abbott, Dexcom, Inc., LifeScan Canada. Speaker’s Bureau; Self; Dexcom, Inc. M. Riddell: Advisory Panel; Self; Zucara Therapeutics Inc. Consultant; Self; Lilly Diabetes. Research Support; Self; Dexcom, Inc., Insulet Corporation. Speaker’s Bureau; Self; Medtronic, Novo Nordisk A/S.

0834 Different Neural Circuits Were Associated With Different Parts of Relationships Among Sleep, Pain and Anxiety in Women with Primary Dysmenorrhoea

Abstract Introduction Although the relationships amongst sleep, pain and anxiety have been evidenced, the underlying neurological mechanisms remain elusive. Primary dysmenorrhea is a good model of spontaneous pain with clear painful (menstruation, state) and pain free (e.g., peri-ovulatory phase, trait) conditions. We sought to investigate the state- and trait-related neural signatures that link sleep and anxiety in primary dysmenorrhea. Methods Thirty female subjects with primary dysmenorrhea and 30 healthy female controls completed resting-state functional magnetic resonance imaging, the State-Trait Anxiety Inventory and sleep quality assessments during menstruation and peri-ovulatory phases. As we have reported that there was altered default mode network in the primary dysmenorrhea, posterior parietal cortex (PPC) was therefore chosen as the seed to elucidate the functional connectivity that may correlate with sleep and anxiety. Results The primary dysmenorrheic subjects exhibited sleep disturbances throughout the menstrual cycle with increased state anxiety. The primary dysmenorrheic subjects demonstrated significantly higher correlations between the sleep quality and the PPC-insula and -putamen functional connectivity during the peri-ovulatory phase. During menstruation, the primary dysmenorrheic subjects demonstrated significantly lower correlations between their state-anxiety scores and the PPC-occipital cortex functional connectivity. Conclusion After regressing out the effects of trait anxiety, the correlation between sleep quality and the PPC-putamen functional connectivity was not significant, indicating anxiety-mediated sleep-pain relationship. The PPC-insula functional connectivity, by contrast, was remained significant. Furthermore, state anxiety was associated with the PPC-occipital cortex functional connectivity that was significantly impaired in primary dysmenorrheic subjects. These findings hinted pain and anxiety mediating sleep quality in different neurological circuits. Support Taipei City Goverment(10401-62-046 and 10501-62-046)

IMPROVING KNOWLEDGE OF ADOLESCENT WOMEN ABOUT MENSTRUATION THROUGH HEALTH PROMOTION

Menstrual Health Promotion Activities for Adolescent Women at the 2nd Junior High School of Buduran, Sidoarjo is a form of community service in the form of counseling aimed at growing and enhancing healthy behavior in adolescents, especially in recognizing the phase of menstruation in adolescent women. The implementation of these activities on December 4, 2018 took place at the 2nd Junior High School of Buduran, Sidoarjo. The target is 7th grade students of the 2nd Junior High School of Buduran, Sidoarjo. Before the activity is carried out, there is a process of compiling the activity for 3 weeks before the activity is carried out, starting from the selection of health counseling materials to the submission of licensing to the relevant parties. As an evaluation, the activity was attended by 44 students and 1 teacher, participants participated in the activity with enthusiasm and conducive, the activity can be carried out on time smoothly. Keywords: Women’s youth knowledge, health promotion, menstruation.

Dose-dependent effects of estrogen on prediction error related neural activity in the nucleus accumbens of healthy young women.

Whereas the effect of the sex steroid 17-beta-estradiol (E2) on dopaminergic (DA) transmission in the nucleus accumbens (NAc) is well evidenced in female rats, studies in humans are inconsistent. Moreover, linear and inverted u-shaped dose response curves have been observed for E2's effects on hippocampal plasticity, but the shape of dose response curves for E2's effects on the NAc is much less characterized. Investigation of dose response curves for E2's effects on DA-related neural activity in the human NAc. Placebo or E2 valerate in doses of 2, 4, 6 or 12 mg was orally administered to 125 naturally cycling young women during the low-hormone menstruation phase on two consecutive days using a randomized, double-blinded design. The E2 treatment regimen induced a wide range of E2 levels, from physiological (2- and 4-mg groups; equivalent to cycle peak) to supraphysiological levels (6- and 12-mg groups; equivalent to early pregnancy). This made it possible to study different dose response functions for E2's effects on NAc activity. During E2 peak, participants performed a well-established reversal learning paradigm. We used trial-wise prediction errors (PE) estimated via a computational reinforcement learning model as a proxy for dopaminergic activity. Linear and quadratic regression analyses predicting PE-related NAc activity from salivary E2 levels were calculated. There was a positive linear relationship between PE-associated NAc activity and salivary E2 increases. The randomized, placebo-controlled elevation of E2 levels stimulates NAc activity in the human brain, likely mediated by dopaminergic processes.

Menstrual cycle-associated effects on some acute phase reactants parameters of Students in Usmanu Danfodiyo University Sokoto, North Western Nigeria.

The menstrual cycle is the cycle of natural variations that occurs in the uterus and ovary as an essential part of making sexual reproduction possible. It is characterized by hormonal changes but the changes that occur in some active phase reactants (APR) parameters have not been fully elucidated. The aim of this study was to compare the serum albumin, ESR, and C-reactive protein levels in follicular and luteal phases of menstruation. A total of 90 healthy regularly menstruating women where used for this study. Forty-five of the study participants were in their follicular phase while the other 45 where in their luteal phase. Four milliliters of blood were withdrawn from each patient under aseptic condition and two milliliters was dispensed into EDTA container while the other two milliliters were dispensed into a plane container. The EDTA anticoagulated blood was used for ESR and full blood count while the serum from the plain tubes was used for analysis of C-reactive protein and Serum Albumin. Sysmex K-3 auto-analyser (Sysmex, Kobe Japan) was used for te determination of full blood count, the Westergren method was used for ESR estimation, Bromo Cresyl Green method was used for serum albumin and ELISA method was used for CRP determination. Data analysis was carried out using SPSS version 23. This study showed a statistically significant difference in the ESR (p= 0.03) among menstruating women in the follicular and luteal phases of menstruation. Sociodemographic factors had no statistically significant effect on the APR parameters of menstruating women in the follicular and luteal phases of menstruation (p> 0.05). There were no statistically significant differences between acute phase proteins of menstruating women in the follicular phase and luteal phases (p> 0.05). Also, age and marital status did not affect the acute phase proteins among menstruating women in the follicular phase and luteal phases (p> 0.05). There is need to generate data on menstrual disorders and their impact on women's health status, quality of life and social integration. It is vital that evaluation and treatment of menstrual complaints should be given a higher priority in primary care programs. There is need to invest in public enlightenment program to increase awareness in secondary schools to increase the level of awareness among adolescents as well as young females.