Water pH Research Articles

Perencanaan Tahapan Persiapan Dan Perhitungan Reklamasi Di Lahan Bekas Tambang Air Rikai PT Timah Tbk Kabupaten Bangka

The research location chosen was the Air Rikai ex-mining land, which is an alluvial ex-mining land within the IUP of PT Timah Tbk. The negative impact that arises from mining is in the form of land damage. This research aims to overcome damage and also ensure that reclamation activities can be carried out well. The method used in this research is a quantitative method. The data taken is in the form of soil samples, soil pH and water pH data, topographic data (x,y,z coordinates) and bathymetric data for the depth of the pit. The results of data processing and analysis show that the condition of the land to be reclaimed has an area of 13 Ha and is divided into 2, namely voids with a total area of 1.57 Ha and land area of 11.43 Ha. Land use is carried out by stockpiling overburden with a required volume of overburden of 35,511.54 m³. Land leveling with a volume of pushed soil of 35,280 m³. Arrangement of the edge of the pit with a volume of soil moved of 230.29 m³. Construction of drainage channels with an excavation volume of 214.59 m³. Installation of concrete buis type culverts with dimensions of 50 x 50 cm and a length of 50 cm. In the revegetation, 1,517 oil palm trees were planted, then 463 sea cypress trees were planted along the underside and LCC seeds to help loosen the soil. Treating acid mine drainage with 350 water hyacinths. The costs required for reclamation are divided into 2, namely, Direct Costs and Indirect Costs. The direct costs required are IDR 679,286,160 and the indirect costs required are IDR 213,975,140. The total costs required for reclamation of land with an area of 13 Ha are IDR 893,261,300.

Flare Pollution Loads and Carbon-Dioxide Effect on Rainwater Acidity in Niger-Delta: A Review, Investigation and Model for Safe Living Quarter

Carbon-dioxide does not only affect climate change, but also contribute tremendously in acidification of rain water. Hazard identification and risk assessment are fundamental components of effective risk management, specifically in sensitive areas where adverse effects can have significant consequences. This study provides novel methodology for environmental and safety assessment of flared gases in sensitive areas such as residential homes. Distancing Sampling Technique (DST) was used to investigate the sensitivity of rain water pH at distances away from flare site in order to develop a Risk Management Model for sensitive regions. First, a review on rain water acidity was made around flaring and non-flaring areas in Niger-Delta states, which revealed Moderate-High acidity effect around flaring zones and no effect on non-flaring zone. Secondly, Flared Gas Quantification, pH Experimental Evaluation (PEE) and Risk Assessment Matrix (RAM) were the three systematic approaches used respectively to quantify, measure and evaluate the effects of CO2 and other flare pollutants around the area of study. An average of 809,300,000 Mscf of associated petroleum gases were flared around the oil and gas producing areas in Delta State, causing a release of around 43x106 tons of CO2 from 2012-2022. Experimental results showed the range of pH from 4.56 ± 0.06 to 5.10 ± 0.06 for the 33 samples of harvested rainwater in Kwale community, Delta state causing a deviation of 16.38 to 30.05% from standard. The developed and validated model suggests 4.81KM radius as the safe distance for human habitation from flare sites. Based on these findings, carbon-capture and sequestration projects must be activated in Niger-Delta to curb the menace.

Laser-Induced Graphene for Electrochemical Analysis of Food and Agricultural Contaminants

Carbon-nanomaterial sensors offer promising routes toward low-cost, high-throughput electrochemical sensors. Carbon nanomaterials, such as graphene and carbon nanotube (CNT), have been studied with various fabrication methods to produce sensors, including high-quality thin film graphene and CNT by chemical vapor deposition. However, the complexity and cost associated with their fabrication have hindered their implementation into single-use test strip sensors. Even high-yield mechanical and/or chemical exfoliation of graphene from graphite followed by solution phase printing of electrodes can be costly due to the complexity of ink formulation and the need for post-print annealing requirements. However, laser-induced graphene (LIG) avoids the need to exfoliate graphene from graphite or to create a solution-phase graphene ink by converting sp3 carbon found in carbon-rich substrates into conductive sp2-hybridized carbon found in graphene through a laser scribing technique. The resulting LIG also exhibits a high surface area and is porous with a high density of edge plane sites, which are beneficial for biofunctionalization and heterogeneous charge transport during electrochemical sensing. Furthermore, we have demonstrated that the surface area and wettability of the LIG can be tuned to greatly improve the sensitivity of electrochemical enzymatic biosensors (detection limits down to the picomolar range) and reduce the water layer buildup between the ion-selective membrane and the electrode, which improves the accuracy of the sensor readings. The fabricated LIG electrodes have been used in a wide variety of electrochemical sensing applications, including in food safety (foodborne pathogens and other contaminants), environmental monitoring (agrochemicals), and health monitoring sensors (salivary potassium and lactate, and urinary potassium and ammonium). Different electroanalytical methods are used, including amperometric, potentiometric, coulometric, and impedimetric, to quantify the analytes. We demonstrate how the ISE (ion-selective electrodes) can be functionalized with PVC-based ion-selective membranes for potassium, nitrate, nitrite, ammonium, sodium, and pH in various biological solutions, including soil and water for nutrient/fertilizer monitoring in farm fields. Additionally, we demonstrate how the hydrophilic LIG graphene can be used to create other highly sensitive electrochemical biosensors including enzymatic pesticide biosensors and Salmonella spp. immunosensors. All these electroanalytical sensors were validated in real-world samples and demonstrated to have limits of detection and linear sensing ranges that are relevant to their sensing applications. Results demonstrate the utility of LIG-based sensors for rapid and sensitive electrochemical measurement of contaminants as a readily modified platform for scalable production of electroanalytical devices applied toward point-of-service environmental and food monitoring.

Consistent responses of the shrimp Litopenaeus vannamei gut microbiota to Enterocytozoon hepatopenaei infection across spatially distant farms

The gut microbiota of aquatic animals is supposed to be sourced from rearing water. However, bacterioplankton communities exhibit a biogeographic pattern, raising the question whether the gut microbiota respond consistently to shrimp health status across spatially distant farms. Here, we collected shrimp Litopenaeus vannamei samples infected with Enterocytozoon hepatopenaei (EHP) from six geographically distant farms with varying local conditions. The gut microbiota of healthy shrimp differed significantly across aquaculture locations, while EHP infection effect was also detectable at each location. Therefore, we ruled out the location effect by removing taxa that featured location. After this optimization, we identified 23 EHP-discriminatory taxa that accurately diagnosed EHP infection, achieving an overall accuracy of 90.5%. Structural equation model revealed that water pH and NO3− levels were the governing factors for the bacterioplankton community. However, the shrimp gut microbiota was not strongly affected by the bacterioplankton community in their rearing water. EHP infection consistently increased deterministic processes and destabilized the biotic network in the shrimp gut microbiota. Collectively, our findings provide evidence that EHP infection causes consistent changes, e.g., altered structure, increased determinism, and destabilized network, in the gut microbiota across spatially distant farms. In particular, we established a diagnosis model for diagnosing EHP infection that is independent of aquaculture location.

Development of Smart Hydroponics System using AI-based Sensing

This paper proposes a smart hydroponic system that operates automatically using a fuzzy logic algorithm, integrating IoT functionalities to support smart agriculture. The system allows for remote monitoring and control via the internet, providing real-time data on water levels, pH levels, temperature, and nutrient solution temperature. Precise dosing and temperature control are critical for optimal plant growth, and the system schedules temperature measurements to ensure stability. Unstable temperature can affect pH levels, thereby impacting nutrient absorption. The proposed system employs sensors to continuously monitor the electrical conductivity (EC) and pH levels of the nutrient solution. Fuzzy control is utilized to regulate the nutrient solution pump, automatically adjusting EC and pH levels to promote optimal plant growth. This approach reduces the time burden on producers and provides more precise control over the nutrient solution, resulting in improved growth outcomes. The main contributions of this work are as follows: the development and implementation of an AI-based system integrating a controller, IoT environment, fuzzy logic algorithm, and NFT (nutrient film technology) hydroponics; the creation of a user-friendly interface for farmers through the Smart-Hydroponic application, enabling hybrid monitoring and control of hydroponic farms; the establishment of an IoT-based cloud environment for sensor data monitoring; the implementation of a smart hydroponic system for nutrient sensing, monitoring, and control; and a comparative analysis between smart and conventional hydroponics based on morphological results.

Skin barrier, phenotypic and genotypic characterisation of autosomal recessive ichthyosis in TGM1-deficient Jack Russell Terriers and response to topical ceramide.

Autosomal recessive ichthyosis leads to structural or biochemical changes that impair skin barrier function. To assess (1) the phenotype and genotype in a litter of Jack Russell Terriers with autosomal recessive congenital ichthyosis (ARCI), and (2) the defective skin barrier and determine if a topical ceramide can modulate the barrier. A healthy dam and litter of Jack Russell Terrier puppies (healthy male, affected male and female), one affected adult Jack Russell Terrier and one unrelated healthy Jack Russell Terrier. A severe cornification defect was identified via examination of affected puppies. As the phenotype worsened, the affected puppies received a topical application of ω-0-acylceramide for 10 days. Before humane euthanasia, the skin barrier was evaluated via transepidermal water loss (TEWL), corneometry and pH in affected dogs. Genomic testing was performed, and skin samples were analysed by light and electron microscopy. Affected puppies were homozygous for the 1980 bp LINE-1 insertion in the TGM1 (transglutaminase 1) gene; the unaffected littermate and the dam were heterozygous carriers. ARCI puppies were underweight and had a severe hyperkeratotic phenotype that impaired mobility. TEWL was markedly higher in affected dogs. The cutaneous pH of affected puppies was higher than the normal littermate. Treatment of the skin with ω-0-acylceramide normalised the pH to match the littermate and decreased TEWL. Electron microscopy revealed marked attenuation of the cornified envelope. Dogs with TGM1-deficient ARCI have an impaired skin barrier. Topical therapy can partially repair the barrier defect.

Effect of Physical Factors on Milt Quality of Malabar Grouper, Epinephelus Malabaricus in Summer and Winter Season

A study was conducted to test the effect of physical factors such as temperature, salinity, and pH on spermatozoa motility of Malabar grouper (Epinephelus malabaricus). Mature and healthy male fishes were collected from the Red Sea during the summer and winter seasons for milt collection. Milt quality was assessed for colour, volume, sperm cell concentration, motility, and viability. Sperm motility was tested under different water temperatures, salinity, and pH media. A significant difference (P < 0.01) in sperm cell concentration (density), sperm motility (%), and viability (%) was observed between the summer and winter seasons. Changes in water temperature, pH, and salinity influenced sperm motility (%) and motility duration (min). Low pH and salinity caused adverse effects on spermatozoa motility. The results of the study are useful for the germplasm conservation of Malabar grouper, E.malabaricus.

Identification of high-performing soil groups in grazing lands using a multivariate analysis method

Understanding and quantifying the complex relationships between soil properties and vegetation health is important for sustainable land management and optimising agricultural productivity. This study tested a spatial data-driven framework to identify the soil groups associated with pasture health using publicly available gridded soil attribute layers from Soil and Landscape Grid of Australia (SLGA) over two adjacent southeast Australian river catchments. Principal component analysis (PCA) followed by isocluster unsupervised classification was applied to seventeen SLGA soil attribute layers to identify dominant soil patterns, which showed good spatial agreement with the Enhanced Vegetation Index (EVI) data derived from Landsat-8 imagery. The soil class demonstrating the highest EVI values (HVR class) and the lowest EVI values (LVR class) were determined. A comparison of these classes with soil types defined in the New South Wales Soil Landscape maps confirmed that the HVR class is predominated by agriculturally productive, basalt-derived 'Ant Hill' soils. The cation exchange capacity (CEC) ranging from ∼10 to 20 meq/100 g, clay content ranging from 20 % to 30 %, pHc (pH in calcium chloride solution) between 5 and 6, pHw (pH in water) between 5.5 and 6.5 and SOC between 2.5 % to 4 % were associated with higher EVI values in the HVR soil class within the study area. This study demonstrated an effective framework for identifying key soil attributes that affect agricultural productivity and pinpointing critical locations for grazing land productivity.

Evaluating Surface Water Quality of Euphrates River in Al-Najaf Al-Ashraf, Iraq with Water Quality Index (WQI)

The present study illustrates the rapid pollution of Euphrates River, inwards Al-Najaf Al-Ashraf governance (Al-Kufa River) in Iraq, which is one of the most important rivers in the region. The river faces formidable pressure due to encroachments, discharge of untreated domestic and industrial waste, drainage water from cultivated orchards, and dumping of solid waste. This study was conducted to assess the temporary and locative organic pollution in the region. Water Quality Index (WQI) of the Al-Kufa river was determined according to the Canadian Council of Ministers of Environment (CCME) method by calculating seven parameters (water temperature, DO, COD, EC, TDS, turbidity, and pH) in two sampling stations. Surface water samples were collected in a period of four months (September to December) in 2023. WQI level, correlation analysis between parameters, and Iraqi and CCME drinking water specification standards were employed to classify the surface water status. The results show that the ecological condition can be classified as marginal with WQI= 49 and poor with WQI= 42 in stations 1 and 2, respectively.

Rough and porous silica-modified fiber membranes decorated with oleic acid for ultra-high flux and pH-responsive oil/water separation

The design of fiber membranes with both ultra-high flux and pH-responsive oil/water separation is challenging but of great significance to environmental protection and human health. In this work, the pH-responsive fiber membranes were prepared by immersing the silica-modified polyacrylonitrile fiber substrates into oleic acid solution. The obtained membranes exhibited highly rough surface structures (Ra = 14.26 µm), large pore sizes (5.35 µm), high porosities (86.04%), and pH-responsive functional groups, respectively. The pH-responsive fiber membranes also had superhydrophobic surfaces and excellent selective separation for oil/water mixtures, for example, a trichloromethane permeation flux of 87,829 L m-2h-1 for immiscible oil/water separation, and a water permeation flux of 69,802 L m-2h-1 after turning the pH of water from 7 to 13. The above data were much higher than those of stimulus-responsive fiber membranes used for oil/water separation. In addition, the pH-responsive fiber membranes were also used for separating water-in-oil emulsions and oil-in-water emulsions, and achieved the highest separation flux of 15,120 L m-2h-1. This work provides a simple strategy for the preparation of a stimulus responsiveness and high-performance material that can be used in the direction of treating practical oily wastewater.

A Method Development to Enhance Water Alkalinity for Reducing Health Risks Associated with Unsafe Water

Background: Water is essential for life, sustaining both biological and human activities. It plays a crucial role in the physiological functions of the human body and shapes human civilization. Water’s unique properties and its pH, a measure of its acidity or alkalinity, influence its quality and impact on health. The pH of drinking water is particularly important for microbial activity and human health. This study aimed to investigate the effectiveness of increasing water alkalinity, with a focus on improving pH levels in tap water through the use of clay pots. Methods: The study was conducted in Sulaymaniyah city, with tap water sourced from the Dokan Dam. Ceramic clay pots, selected for their availability and affordability, were used to treat the tap water. The experiment measured the pH of untreated tap water and compared it to water stored in clay pots over a 24-hour period for 12 weeks. A paired t-test was used to analyze the difference in pH levels between untreated and treated water across two seasons, autumn and winter. Results: The results showed a significant increase in the pH levels of tap water after treatment with clay pots. In autumn, pH values rose by an average of 2.86, while in winter, the increase averaged 2.66. Statistical analysis confirmed highly significant differences in pH between untreated and treated water for both seasons (p = 0.000002), demonstrating the effectiveness of clay pots in raising water alkalinity. Conclusion: The study concluded that clay pots offer a simple and cost-effective method to increase the alkalinity of tap water, improving its quality. This approach can benefit public health, particularly in regions with limited access to advanced water treatment technologies. Further research is needed to explore the long-term effects and broader applications of using clay pots for water treatment.

Design and Development of Automated Nutrient Regulation System for Hydroponics Unit

Hydroponics is a method of growing plants without soil. Hydroponics, a subset of hydro culture, is a growing plant without soil, using mineral nutrient solutions in a water solvent. In soil less culture especially, hydroponics, the problem of regular maintenance of nutrient solution and required technical knowledge. The automation in hydroponics further helps to boom up the hydroponic cultivation. Automated nutrient regulation in hydroponics environment with automatic nutrient feed technology was developed and tested in the present study. The experiment was carried out an automated controlling and regulation of pH, TDS, temperature in polyhouse hydroponic system using Arduino-Uno controller, solenoid valves containing nutrients, water, pH-up, and pH-down and sensors. In this study, the sensors are connected to Arduino-Uno controller in which liquid crystal display (LCD) of Arduino-Uno circuit controls and regulates the system based on the coding which has been programmed previously. Calibration was done for all the parameters in the automated system. TDS and pH values of nutrient solution were recorded by using TDS and pH sensor probes respectively were fed to Arduino which adjusts the values, if required, through actuators. The prototype model was able to monitor and control the pH and TDS of hydroponic water in the required range with high accuracy.

Effects of earthworm and arbuscular mycorrhizal fungal inoculation on carbon component accumulation and allocation in rocky desertification soils.

Exploring the responses of carbon component accumulation and allocation to arbuscular mycocorrhizal fungi (AM) and earthworm inoculation can provide reference for improving carbon sequestration potential and bioremediation efficiency in rocky desertification soils. In this study, we chose Fraxinus malacophylla as the host plant to inoculate with Funneliformis mosseae (FM), earthworm (E), and E+FM, using no earthworm and mycorrhizae addition as CK to examine the spatiotemporal variations in soil carbon components (i.e., total organic carbon, microbial biomass carbon, easily oxidized organic carbon, and recalcitrant organic carbon) and their allocation (i.e., microbial biomass carbon/total organic carbon, easily oxidized organic carbon/total organic carbon, and recalcitrant organic carbon/total organic carbon). The results showed that 1) The respective and interactive inoculation of E and AM significantly promoted the accumulation of each carbon component. In contrast with the control, the average carbon component levels under three inoculation treatments were ranked as E+FM>E>FM. The three inoculation treatments significantly promoted soil microbial carbon/total organic carbon (30.5%-68.5%) and easily oxidized carbon/total organic carbon (31.2%-39.2%), but decreased recalcitrant organic carbon/total organic carbon (2.9%-16.2%). 2) The spatiotemporal variation in accumulation and allocation of soil carbon components varied between the inoculation treatments. The maximum value of each carbon component occurred in June. The increase in each carbon component was significantly higher in E+FM (33.0%-122.1%) than that in E (31.2%-95.4%) and FM (9.2%-41.3%). The maximum value of microbial biomass carbon/total organic carbon and easily oxidized organic carbon/total organic carbon was observed in June, while that of recalcitrant organic carbon/total organic carbon was recorded in December. In contrast with CK, the amplitude of variation in the proportion of carbon components in total organic carbon under the three inoculation treatments was ranked as E+FM>E>FM. The accumulation and allocation of all carbon components decreased (9.7%-146.2%) along the soil profile. The level of carbon components in the E treatment decreased the smallest. The microbial biomass carbon/total organic carbon and easily oxidized carbon/total organic carbon decreased the least and the recalcitrant organic carbon/total organic carbon decreased the greatest under the E+FM treatment. 3) Changes in soil physicochemical properties under the three inoculation treatments significantly affected the accumulation and allocation of organic carbon components. Soil pH was negatively correlated with carbon component accumulation and allocation, whereas other soil variables were positively correlated with them. 4) The results of principal component analysis showed that soil water content, total nitrogen, and total phosphorus were the main factors driving carbon component accumulation, while soil water content, total phosphorus, and pH were the main factors controlling carbon component allocation. Therefore, we concluded that the earthworms, AM fungi and their interaction affected the accumulation and allocation of carbon components in Yunnan rocky desertification soils, which would primarily depend on the changes of soil water content, acid-base property, as well as nitrogen and phosphorus conditions.

Novel Sensor Using ISFET and Pt Electrodes for Water pH and Flow Speed Measurement

Novel Sensor Using ISFET and Pt Electrodes for Water pH and Flow Speed Measurement

Synthesis of Cellulose-Based Hydrogel-Nanocomposites for Medical Applications.

This study focused on synthesizing a cellulose-based hydrogel nanocomposite as a green hydrogel by adding a microcrystalline cellulose (MC) solution to carboxymethyl cellulose sodium (CMC-Na) with citric acid as a cross-linker. Y2O3 nanoparticles were incorporated during hydrogel preparation in different ratios (0.00% (0 mmol), 0.03% (0.017 mmol), 0.07% (0.04 mmol) and 0.10% (0.44 mmol)). FTIR analysis confirmed the cross-linking reaction, while XRD analysis revealed the hydrogels' amorphous nature and identified sodium citrate crystals formed from the reaction between citric acid and CMC-Na. The swelling test in deionized water (pH 6.5) at 25 °C showed a maximum swelling percentage of 150% after 24 h in the highest nanoparticle ratio. The resulting cellulose hydrogels were flexible and exhibited significant antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). The synthesized cellulose-based hydrogel nanocomposites are eco-friendly and suitable for medical applications.

Physical and chemical properties and sensory evaluation of camel meat and new camel meat jerky.

Alxa Bactrian camel meat is an organic diet that provides balanced nutrition and is easy to digest and absorb. Despite its potential, it is currently underutilized. To develop a new type of camel jerky, this study utilized a single-factor design method to optimize the formula and fermentation process parameters of Alxa Bactrian camel jerky. Additionally, the physical and chemical composition, nutritional components, protein degradation, and microbial changes of the camel jerky were analyzed to evaluate the nutritional benefits of the new camel jerky created using modern fermentation technology. The results show that the optimal addition amounts of camel fermented jerky ingredients are 2.2% black pepper, 3% salt, 1.8% white sugar, and 9% cooking wine, whereas the optimal fermentation conditions by compound starter culture are 0.07% starter, 23 h of fermentation time, and a 30°C fermentation temperature. Compared to the single starter, the compound starter significantly increased the protein content, total amino acids, unsaturated fatty acids, trace elements, and a*, L*, and e-values of jerky; however, it also decreased the water activity (a w), thiobarbituric acid, and pH values of jerky during storage. Compound starter strains can compensate for the shortage of single starters and improve the overall quality of meat products. These findings could provide new insights and serve as a reference for the development of camel meat products and hold significant importance for the growth of the camel industry.

Comparing Conventional and Modern Methods for The Phycocyanin Extraction from Spirullina sp

Spirulina platensis, a blue-green algae abundant in tropical regions, is rich in minerals, vitamins, fibers, and pigments, with low nucleic acid content. It has unique chromoproteins called phycobiliproteins, notably phycocyanin, used in various applications. This study aims to optimize phycocyanin extraction using different solvents (distilled water and sodium phosphate buffer pH 6.7) and methods (freeze-thaw and sonication). Spirulina platensis biomass was extracted in both solvents, then some of them was freeze for 24 and 48 hours followed by thawing overnight. The other was sonicated for 2.5 minutes, 50 Hz then soaked for 1, 2, and 3 hours. All of the samples were centrifuged at 6000 rpm for 10 minutes and the absorbance was measured using a UV-Vis spectrophotometer at wavelengths of 280, 620, and 650 nm. with freeze-thawing for 48 hours yielded the highest phycocyanin concentration (0.55%), with a yield of 11.07 and purity of 0.21. Sonication improved phycocyanin concentration, yield, and purity significantly, yielding 1.108, 25.85, and 0.26, respectively.

Versatility of Juçara (Euterpe edulis) extracts as corrosion inhibitors of mild steel in different environments: 1 mol L−1 HCl and CO2-saturated formation water

Developing a sustainable and environmentally friendly inhibitor remains an essential and challenging goal for corrosion control. Here, different extracts from the same biomass (Juçara - Euterpe edulis) were applied as a new inhibitor for mild steel in different corrosive medium: 1 mol L−1 HCl (pH 0) and simulated CO2-saturated formation water (pH 5). Depending on the extraction process, the anticorrosive capacity of extracts may increase, a fact verified in gravimetric tests, reaching 94.4% maximum inhibition efficiency for the acidic medium (1000 mg L−1 of Juçara pulp in 48 h of immersion) and 86.1% inhibition efficiency for the saline medium (600 mg L−1 of Juçara pulp extract assisted by ultrasound in 24 h of immersion). Gravimetric tests, electrochemical tests, and surface and biochemical characterization analyses were carried out to better understand these extracts' performance. This work provides an interesting application of the same biomass effectively acting in different corrosive medium, depending on the extraction method.

Remediation of Heavy Metal Pollution from Coal Mine Effluent Using Metal-Organic Frameworks (MOF): Impact of Water Media, Operational Factors and Metal Characteristics

The energy sector is the sector that generates the highest amount of environmental contamination, especially in water sources, mostly in the case of coal-based energy production. The aim of this study was to examine a significant contamination source, heavy metal contamination, in coal mining effluents. The current investigation introduces an MOF platform based on zirconium clusters and isophthalic acid with NH2-MIP-SO3H mixed amine and sulfonic acid functional groups in order to remove the most common heavy metal ions in coal mining effluents, including Hg, Cd, Pb, and Cu ions. The water matrix and the operational conditions were identified to be very influential in the removal process, such as the pH of water, the initial metal concentration and operating time. NH2-MIP-SO3H offers a great removal efficiency of metals starting from 745.83 mg/g for Cd, 673.67 mg/g for Cu, 589.85 mg/g for Hg, and 481.66 mg/g for Pb ions, with the Langmuir equation for equilibrium and pseudo-second-order equation for kinetics being the ideal models to express the equilibrium and kinetic data, respectively. A significant impact of water pH was found to occur, with the NH2-MIP-SO3H platform performing best at pH 6. Reuse of NH2-MIP-SO3H demonstrates excellent reusability, sustaining 90% of initial performance over eight regeneration cycles. The interaction of functional group-functional metal was the dominant mechanism in the removal process. The NH2-MIP-SO3H unique approach to heavy metal removal provides a very hopeful outlook for additional investigations in larger-scale studies.

Evaluation of analytical greenness metric for an eco-friendly method developed through the integration of green chemistry and quality-by-design for the simultaneous determination of Nebivolol hydrochloride, Telmisartan, Valsartan, and Amlodipine besylate

In recent years, the field of analytical chemistry has witnessed a notable shift towards the adoption of greener chromatographic methods, aiming to minimize the environmental impact. An effective strategy involves substituting conventional harmful organic solvents with environmentally friendly alternatives, reducing the use of hazardous chemicals that contribute to environmental concerns. However, separating drug substances without the use of buffers and organic solvents presence is a big challenge. To overcome this challenge, a combination of quality-by-design (QbD) and green analytical chemistry (GAC) was employed in this study for method development. A high-performance liquid chromatography (HPLC) method was successfully developed and validated for the simultaneous determination of Nebivolol hydrochloride, Telmisartan, Valsartan, and Amlodipine besylate. The method utilized a mobile phase composed of a mixture of 0.1 % formic acid in water (pH: 2.5) and ethanol. A regular octadecyl silica (ODS) column was employed, and UV detection at 220 nm was utilized. The method exhibited linearity within the concentration range of 25–75 μg/mL for Telmisartan and 150–450 μg/mL for Nebivolol Hydrochloride, Valsartan, and Amlodipine besylate and the correlation coefficient was greater than 0.999 for all the analytes. Limits of detection (LOD) and quantification (LOQ) were determined as 0.01 and 0.04 μg/mL for Telmisartan, 0.06 and 0.20 μg/mL for Nebivolol Hydrochloride, 0.08 and 0.25 μg/mL for Amlodipine besylate, and 0.14 and 0.46 μg/mL for Valsartan, respectively. The developed method underwent thorough validation, encompassing various parameters such as linearity, accuracy, precision, LOD, LOQ, robustness, and ruggedness. The mean recovery values were observed to range between 98.86 % and 99.89 %. The accuracy demonstrated was consistently above 98.98 % for both intra-day and inter-day precisions were with the relative standard deviations less than 2 %. To establish its robustness, a quality-by-design-based experimental design (DoE) approach was implemented. Additionally, the method's environmental friendliness was evaluated using the Analytical Greenness metric (AGREE) an analytical eco scale, both confirming its alignment with sustainable practices and reduced ecological impact. The sustainability of the solvent used in the current study was evaluated by Green Solvents Selecting Tool (GSST) Further, the developed method greenness was evaluated with the green analytical tools such as Analytical method greenness score (AMGS) and using the recently released White Analytical Chemistry (WAC) using RGB assessment tool. By employing this greener approach to chromatography method, this study contributes to the ongoing efforts in analytical chemistry to promote sustainable practices and minimize the environmental footprint of analytical methods.