Modulating electrode area in microbial fuel cell enhanced floating beds: synergistic effects on bioelectricity generation and perfluorooctanoic acid degradation.

Peroxisome proliferator-activated receptor gamma (PPAR-γ) is a key regulator of adipose tissue expansion, coordinating adipocyte physiological differentiation and maturation. In this study we evaluated the PPAR-γ effects of perfluorooctanoic acid (PFOA), a perfluorinated alkylated environmental obesogen, associated with an increased risk of diabetes, during adipogenic differentiation of 3T3-L1 murine cells. In particular, using in vitro monoculture (differentiating preadipocytes exposed to PFOA) or co-culture systems (differentiating preadipocytes in contact with PFOA-induced dysfunctional mature adipocytes) we examined adipocytes morphology and biological functions such as adipogenesis, insulin sensitivity and lipolytic response to adrenergic compounds. Exposure to PFOA (0.1-10μM) promoted adipocyte hypertrophy inducing the formation of larger lipid vacuoles. These effects were also evident in the co-culture system, where paracrine factors released by PFOA-induced hypertrophic adipocytes influenced preadipocytes differentiation. Furthermore, both in the mono-culture and in the co-culture systems, PFOA exposure induced a metabolically unhealthy adipocyte phenotype affecting insulin sensitivity. Finally, findings obtained in mono-culture system evidence that these effects induced by PFOA may be related to its capability to affect lipid metabolism and pro/anti-inflammatory adipokine expression. This study, therefore, draws attention on PFOA as an obesogenic factor promoting adipocyte metabolic dysregulation and dysfunctional paracrine signaling.

Differential toxicity of perfluorooctane sulfonate (PFOS) in wild-type and Oatp1d1 mutant zebrafish larvae.

Hexafluoropropylene oxide trimer acid is an unsafe substitute to perfluorooctanoic acid: The perspectives of intestinal microflora and hepatotoxicity in frog.

Health effects of PFASs on five major human cancers: A network toxicology perspective on molecular pathogenesis.

Presence of per- and polyfluoroalkyl substances (PFASs) in the Nandu River Estuary, Hainan Island.

Micro/nanoplastics enhance multigenerational reproductive toxicity of legacy and alternative per- and polyfluoroalkyl substances in the marine rotifer Brachionus plicatilis.

Development of an air sampling method for quantifying 12 legacy and emerging PFAS with the OSHA Versatile Sampler (OVS) tube and analysis by HPLC-MS/MS.

Spatiotemporal distribution of legacy and alternative per- and Polyfluoroalkyl substances (PFASs) in major rivers of the Pearl river delta.

Seasonal dynamics of emerging and legacy PFAS in the Beibu Gulf: Insights from a combined source apportionment and predictive modeling approach.

Per- and polyfluoroalkyl substances (PFASs) profile in sludge biosolid fertiliser intended for agricultural use in Japan.

Conflicting results of ecological and health risk assessment of perfluorinated compounds in major river basins in China.

Sex-dependent acute oral toxicity and in vivo distribution of 6:2 chlorinated polyfluorinated ether sulfonate (6:2 Cl-PFESA) in rats.

Deep defluorination of perfluorooctanoic acid via synergistic enrichment-oxidation using copper-doped nanoscale zero-valent iron: Roles of copper and insights in environmental matrices

Algal-bacterial granular sludge confronting perfluorooctanoic acid: integrated performance and antibiotic resistance genes dynamics

Revealing overlooked per- and polyfluoroalkyl substances (PFAS) in the Pearl River estuary using a comprehensive analytical approach.

Despite the growing concern over per- and polyfluoroalkyl substances (PFAS) exposure in companion animals, dietary intake through commercial pet food remains poorly characterized. In this study, we conducted a comprehensive analysis of 100 commercially available pet food products for dogs and cats in Japan, encompassing both dry and wet types, to quantify 34 target PFAS compounds. PFAS were frequently detected, with concentrations varying by food type, ingredients, and country of origin. Fish-based products contained elevated levels of perfluorooctanesulfonic acid (PFOS), perfluoroundecanoic acid (PFUnDA), and perfluorotridecanoic acid (PFTrDA), and regional patterns suggested the influence of Asian-specific PFAS sources such as F-53B. Hazard quotient (HQ) assessments based on EFSA tolerable weekly intakes indicated that average HQs exceeded 1 for both dogs and cats in several products, signaling potential health risks. However, due to the lack of species-specific toxicokinetic information for dogs and cats, the EFSA-based risk characterization presented here should be interpreted as a preliminary assessment. Although dry food had higher PFAS concentrations when converted to feeding amounts, estimated exposure was higher for wet food due to higher consumption. These findings provide the first evidence of ingredient-driven and country-of-origin-dependent PFAS contamination in pet food. Fish used as ingredients are a significant source of exposure to PFAS. They also highlight the urgent need for regulatory oversight and toxicological evaluations specific to companion animals. These animals serve as vulnerable populations and as sentinels of human co-exposure in domestic environments.

A mediator-free microbial electrochemical biosensor based on Escherichia coli nucleotide metabolism for rapid toxicity assessment of emerging contaminants.

Atmospheric deposition and co-occurrence patterns of organophosphate flame retardants (OPFRs) and perfluorooctanoic acid (PFOA) in urban snow.

Tracking the impact of PFAS regulations: Long-Term analysis of roe deer livers from 1998 until 2022.