Abstract Open-pollinated progeny from 15 peach (Prunus persica) cultivars, two peach × P. kansuensis hybrids, and one peach almond (P. amygdalus) hybrid were evaluated for their cold hardiness and for tolerance to Cytospora canker following artificial inoculation with Leucostoma persoonii. Winter hardiness was negatively correlated with canker necrotic length (r = −0.26**) and positively correlated with canker rating (r = 0.26**), as indicated by qualitative ratings. The half-sib families differed for canker necrotic length following fall inoculation, indicating that individuals with increased tolerance to L. persoonii canker could be selected from the population. Progeny from the cultivar Yennoh exhibited the shortest canker necrotic length following fall inoculation, and all the inoculated branches were visually healthy. ‘Yennoh’, a plant introduction from Russia, may have a higher tolerance to Leucostoma than has previously been found in U.S. germplasm.