Training Patterns Research Articles

Dietary Patterns, Gut Microbiota and Sports Performance in Athletes: A Narrative Review.

The intestinal tract of humans harbors a dynamic and complex bacterial community known as the gut microbiota, which plays a crucial role in regulating functions such as metabolism and immunity in the human body. Numerous studies conducted in recent decades have also highlighted the significant potential of the gut microbiota in promoting human health. It is widely recognized that training and nutrition strategies are pivotal factors that allow athletes to achieve optimal performance. Consequently, there has been an increasing focus on whether training and dietary patterns influence sports performance through their impact on the gut microbiota. In this review, we aim to present the concept and primary functions of the gut microbiota, explore the relationship between exercise and the gut microbiota, and specifically examine the popular dietary patterns associated with athletes' sports performance while considering their interaction with the gut microbiota. Finally, we discuss the potential mechanisms by which dietary patterns affect sports performance from a nutritional perspective, aiming to elucidate the intricate interplay among dietary patterns, the gut microbiota, and sports performance. We have found that the precise application of specific dietary patterns (ketogenic diet, plant-based diet, high-protein diet, Mediterranean diet, and high intake of carbohydrate) can improve vascular function and reduce the risk of illness in health promotion, etc., as well as promoting recovery and controlling weight with regard to improving sports performance, etc. In conclusion, although it can be inferred that certain aspects of an athlete's ability may benefit from specific dietary patterns mediated by the gut microbiota to some extent, further high-quality clinical studies are warranted to substantiate these claims and elucidate the underlying mechanisms.

Comprehensive Bio-Screening of Phytochemistry and Biological Capacity of Oregano (Origanum vulgare) and Salvia triloba Extracts against Oral Cariogenic and Food-Origin Pathogenic Bacteria.

This study utilized phytochemical screening to conduct the qualitative analysis of plant extracts, aiming to identify various classes of secondary metabolites. Moreover, the antibacterial activity of different types of Oregano vulgare and Salvia triloba extracts was determined. To achieve the aim of this study, aqueous, ethanolic, and enzymatic extracts were prepared and screened for phytochemical capacity and antioxidant activities. The determination of the antibacterial activity included phenotypic screening of antibiotic susceptibility pattern of oral and food pathogenic bacterial strains, determination of the minimum inhibitory concentration and minimum bactericidal concentration-via microdilution broth test and in vitro valuation of antibacterial efficacies-of the anti-biofilm properties of the studied herbal extractions. Results: Our study evaluated the phytochemical composition and the antioxidant, antibacterial, and anti-biofilm properties of O. vulgare and S. triloba extracts. The analyzed samples contained bioactive compounds, such as phenolics and flavonoids, contributing to the observed strong antioxidant effect. Furthermore, they exhibited notable activity against oral biofilm formation and demonstrated significant antibacterial efficacy against dental caries' microorganisms as well as food pathogens. Despite methodological variations, all extracts showed significant antioxidant capacity and promising antibacterial activity against various pathogens, including resistant strains, while also inhibiting biofilm formation. Although limited to two plant species and facing methodological constraints, this study lays the groundwork for future research, indicating the therapeutic potential of O. vulgare and S. triloba extracts. Further exploration is needed to report on underlying mechanisms and validate efficacy through clinical trials.

The role of atrial pacing site in reducing filling pressures by accelerated pacing in virtual HFpEF patients

Abstract Background Patients with heart failure with preserved ejection fraction (HFpEF) and pacemakers have been shown to benefit from moderately accelerated pacing rates compared to the standard 60bpm. HFpEF patients often present with prolonged P-wave duration (PWD) and atrial fibrillation. Right atrial appendage pacing (RAAP) lengthens PWD, RA septal (RASP) has a neutral effect, while Bachmann’s bundle pacing (BBP) normalizes or shortens PWD compared to sinus rhythm. The mechanical and hemodynamic effects of atrial pacing site selection remain unknown. Aim To investigate the impact of RAAP, RASP and BBP on cardiac mechanics and hemodynamics in an established cohort of virtual HFpEF patients undergoing accelerated pacing. Method HFpEF with prolonged PWD was simulated using the validated CircAdapt computational model of the human cardiovascular system. First, left ventricular (LV) diastolic dysfunction was simulated by decreasing LV myocardial relaxation rate and compliance, such that mean left atrial (LA) pressure (mLAP) was 15mmHg. Second, PWD was prolonged by prescribing a gradual electrical activation delay from RA to LA of 140ms. We then simulated atrial pacing with three different lead positions (Fig.1, left) and gradually increased pacing rate with a constant 120-ms paced atrioventricular (AV) delay. The CircAdapt model computed regional LA strain and mLAP with RAAP, RASP, and BBP. Cardiac output (5L/min) and mean arterial pressure (92mmHg) were kept constant in all simulations. Results Simulated BBP demonstrated the most synchronous LA mechanical activation and function, characterized by uniform segmental LA strain patterns and highest LA reservoir strain (LASR) (Fig.1, middle, with colored arrows highlighting the mechanical dyssynchrony). Hemodynamically (Fig.1, right), BBP acutely decreased mLAP (-1.0mmHg) at the initial heart rate of 60bpm, while RAAP increased it (+0.3mmHg). As pacing rate accelerated, BBP reduces filling pressure (mLAP: -2.8mmHg), more than RASP (-1.8mmHg) or RAAP (-1.2mmHg) at 80bpm. At high pacing rates, RASP and RAAP adversely increase mLAP, due to the prolonged atrial contraction duration and shorter LV filling time. Conclusion Our simulations of different atrial pacing sites in virtual HFpEF patients suggest that BBP yields the most synchronous LA activation pattern and a more substantial reduction in filling pressures with accelerated atrial pacing compared to RASP. Reduction in mLAP with accelerated pacing was attenuated with RAAP. While our study concentrated on a specific paced AV interval, the favorable outcomes observed with BBP could be further improved by including different, rate-adaptive, AV protocols.Fig 1.

Endogenous Type I-C CRISPR-Cas system of Streptococcus equi subsp. zooepidemicus promotes biofilm formation and pathogenicity.

Streptococcus equi subsp. zooepidemicus (SEZ) is a significant zoonotic pathogen that causes septicemia, meningitis, and mastitis in domestic animals. Recent reports have highlighted high-mortality outbreaks among swine in the United States. Traditionally recognized for its adaptive immune functions, the CRISPR-Cas system has also been implicated in gene regulation, bacterial pathophysiology, virulence, and evolution. The Type I-C CRISPR-Cas system, which is prevalent in SEZ isolates, appears to play a pivotal role in regulating the pathogenicity of SEZ. By constructing a Cas3 mutant strain (ΔCas3) and a CRISPR-deficient strain (ΔCRISPR), we demonstrated that this system significantly promotes biofilm formation and cell adhesion. However, the deficiency in the CRISPR-Cas system did not affect bacterial morphology or capsule production. In vitro studies showed that the CRISPR-Cas system enhances pro-inflammatory responses in RAW264.7 cells. The ΔCas3 and ΔCRISPR mutant strains exhibited reduced mortality rates in mice, accompanied by a decreased bacterial load in specific organs. RNA-seq analysis revealed distinct expression patterns in both mutant strains, with ΔCas3 displaying a broader range of differentially expressed genes, which accounted for over 70% of the differential genes observed in ΔCRISPR. These genes were predominantly linked to lipid metabolism, the ABC transport system, signal transduction, and quorum sensing. These findings enhance our understanding of the complex role of the CRISPR-Cas system in SEZ pathogenesis and provide valuable insights for developing innovative therapeutic strategies to combat infections.

Influence of lateral variations in décollement strength on the structure of fold-and-thrust belts: Insights from viscous wedge models

Fold-and-thrust belts (FTBs) evolve over a mechanically weak basal décollement that separates the overlying intensely deformed rocks from the underlying less deformed ones. Although deformation structures in FTBs commonly show lateral continuity, a closer inspection reveals distinctive variations in structural style (e.g., fold style) along and across mountain belts. This study uses laboratory-scale viscous models to investigate the influence of lateral décollement strength variations on the spatio-temporal evolution of strain patterns in FTBs. These experiments, simulating crustal-scale deformation, show notable changes in the mode of tectonic wedge growth, including the topographic evolution and ductile strain pattern distribution. For example, the deformation front propagates faster over weakly coupled décollement than the laterally adjacent strongly coupled segment, leading to along-strike variations of the topographic slope and curved outline of the deformation front. Constrictional strain, characteristic of regions of weak coupling, is transient and replaced by flattening strain beyond ∼20 % bulk shortening. The latter prevails in regions over strong décollement, whereas complex strain histories mark the transition zone between weak and strong décollements. Based on our modelling results, we propose that variations in décollement strength may cause the segmentation of deformation processes and the development of transverse faults in FTBs.

Kinematics of Two Special Endurance Trials: A Methodological Contribution to 400-m Performance.

This study aimed to determine changes in the kinematics of sprint steps based on progressive muscular fatigue during high-intensity 350-m and 500-m trials. Twelve elite healthy male 400-m sprinters with a minimum of six years of regular sprint training experience were recruited. They were divided into two groups for the experiment: a 350-m and a 500-m trial group. Time and kinematics of sprinting step motion for specific segments, i.e., starting to final stages of each trial, were obtained using the Opto Jump-Microgate optical measurement system. The starting phase of each sprint was defined as the section without muscular fatigue (noF), and the final phase was the sprint under muscular fatigue (onF). Each last 25 m of the 50-m evaluated section containing ten complete running steps was selected for detailed statistical analysis. Various patterns of temporal and spatial variables of sprinting efforts were observed between 350-m and 500-m trials. Each trial result was influenced by significant individual changes (p < 0.05). All variables indicated that the two distances differed significantly in terms of running kinematics. This was confirmed by significant differences in the mean step frequency (p < 0.001), which presented a difference of 11.75%, and the mean step speed (p < 0.001). As a result of these changes, a hierarchical intermittent endurance training pattern was defined. The research concluded that special endurance (intermittent sprints) based on 350 m differed significantly in kinematics from sprints over 500 m. Therefore, it should be assumed that the distance of 350 m is more similar in its kinematics to the 400-m competition. This should encourage coaches and athletes to apply a 350-m distance in training developing special endurance, especially in the pre-competitive and competitive periods.

Engineering the Band Topology in a Rhombohedral Trilayer Graphene Moiré Superlattice.

Moiré superlattices have become a fertile playground for topological Chern insulators, where the displacement field can tune the quantum geometry and Chern number of the topological band. However, in experiments, displacement field engineering of spontaneous symmetry-breaking Chern bands has not been demonstrated. Here in a rhombohedral trilayer graphene moiré superlattice, we use a thermodynamic probe and transport measurement to monitor the Chern number evolution as a function of the displacement field. At a quarter filling of the moiré band, a novel Chern number of three is unveiled to compete with the well-established number of two upon turning on the electric field and survives when the displacement field is sufficiently strong. The transition can be reconciled by a nematic instability on the Fermi surface due to the pseudomagnetic vector field potentials associated with moiré strain patterns. Our work opens more opportunities to active control of Chern numbers in van der Waals moiré systems.

A methodological approach for collecting simultaneous measures of muscle, aponeurosis, and tendon behaviour during dynamic contractions.

Skeletal muscles and the tendons that attach them to bone are structurally complex and deform non-uniformly during contraction. While these tissue deformations dictate force production during movement, our understanding of this behaviour is limited due to challenges in obtaining complete measures of the constituent structures. To address these challenges, we present an approach for simultaneously measuring muscle, fascicle, aponeurosis, and tendon behaviour using sonomicrometry. To evaluate this methodology, we conducted isometric and dynamic contractions in in situ rabbit medial gastrocnemius. We found comparable patterns of strain in the muscle belly, fascicle, aponeurosis, and tendon during the isometric trials to those published in the literature. For the dynamic contractions, we found that our measures using this method were consistent across all animals and aligned well with our theoretical understanding of muscle-tendon unit behaviour. Thus, this method provides a means to fully capture the complex behaviour of muscle-tendon units across contraction types.

The gut microbiome, resistome, and mycobiome in preterm newborn infants and mouse pups: lack of lasting effects by antimicrobial therapy or probiotic prophylaxis

BackgroundEnhancing our understanding of the underlying influences of medical interventions on the microbiome, resistome and mycobiome of preterm born infants holds significant potential for advancing infection prevention and treatment strategies. We conducted a prospective quasi-intervention study to better understand how antibiotics, and probiotics, and other medical factors influence the gut development of preterm infants. A controlled neonatal mice model was conducted in parallel, designed to closely reflect and predict exposures. Preterm infants and neonatal mice were stratified into four groups: antibiotics only, probiotics only, antibiotics followed by probiotics, and none of these interventions. Stool samples from both preterm infants and neonatal mice were collected at varying time points and analyzed by 16 S rRNA amplicon sequencing, ITS amplicon sequencing and whole genome shotgun sequencing.ResultsThe human infant microbiomes showed an unexpectedly high degree of heterogeneity. Little impact from medical exposure (antibiotics/probiotics) was observed on the strain patterns, however, Bifidobacterium bifidum was found more abundant after exposure to probiotics, regardless of prior antibiotic administration. Twenty-seven antibiotic resistant genes were identified in the resistome. High intra-variability was evident within the different treatment groups. Lastly, we found significant effects of antibiotics and probiotics on the mycobiome but not on the microbiome and resistome of preterm infants.ConclusionsAlthough our analyses showed transient effects, these results provide positive motivation to continue the research on the effects of medical interventions on the microbiome, resistome and mycobiome of preterm infants.

Dynamic stress analysis of rock joints under railway loading

The proper estimation of stresses generated by the passage of a train is fundamentally important to the serviceability and longevity of railways, and yet very limited knowledge is available where the track substructure is built on a jointed rock mass. The present study introduces an analytical solution for estimating the ground stresses arising from moving wheel loads, causing a change in the three-dimensional stress state in the track formation, in relation to the stress variation with depth and along the longitudinal track section – that is, the direction of train passage. Based on 21 case histories, an array of field measurements and numerical simulations covering a wide range of freight tonnage, train speeds and different formation conditions were considered to validate the proposed analytical solution. The proposed methodology (analytical solution) was then applied to a jointed rock subgrade to determine the normal and shear stresses acting along a specific discontinuity plane. The main analytical outcome demonstrates that the orthogonal vertical and shear stresses present different and phase-shifted history plots for homogeneous ground conditions with principal stresses rotation. However, conversely for a jointed subgrade, the normal and shear stresses along the discontinuity have the same history plot pattern and are in phase. As a practical guide, the results from this study would help to define which cyclic loads should be applied in laboratory tests to simulate realistic traffic patterns of trains travelling over a jointed rock subgrade.

Design of an enhanced spatial resolution indoor plantar strain and gait analysis setup for biomedical applications

In the recent years, measurement of plantar strain and gait analysis has gained huge attention and plays a pivotal role in monitoring posture related ailments or Diabetic Foot Ulcer (DFU). Fibre Bragg Grating Arrays (closely spaced FBGs), another category of optical sensors, are employed in this study to understand the strain and gait of human foot. These arrays offer critical advantages such as enhanced sensitivity, data acquisition, multiplexing abilities, and sensor location effect compensation. For experimentation, five arrays were distributed among different regions (Upper, Medial, and Heel) of foot and their strain patterns for six volunteers (three male and three female) were recorded for eighty seconds. The data was analysed in two ways, Combined (data of array sensors were averaged to target the whole area) and Individual (for independent analysis of each sensor in array). Incoherent transitions and strain patterns of FBGs within the same arrays observed in individual analysis, explains enhanced resolution capabilities of the arrays. Strain mapping of sensor behaviour also confirmed the identification of various forms of gait i.e., Heel Strike, Flat Foot, Heel Off, and Zero Contact. The average standard deviation values for the arrays was reported below 0.16.

Transmission patterns of multiple strains producing New Delhi metallo-β-lactamase variants among animals and the environment in live poultry markets

The widespread transmission of blaNDM among livestock and the live poultry industry attracts considerable public attention. However, studies have not yet addressed its impact on public health in live poultry markets (LPMs). Herein, we investigated the prevalence and genomic epidemiology of blaNDM-positive bacteria in various niches, and explored the transmission patterns of blaNDM within LPMs. Samples were collected between 2019 and 2022 from two LPMs in China. blaNDM was most prevalent in wastewater (35/66, 53.03%). All vegetable samples were negative for blaNDM. blaNDM was mainly distributed among Escherichia coli (266/336, 79.17%), Klebsiella pneumoniae (62/336, 18.45%), and Acinetobacter baumannii (3/336, 0.89%). Some novel hosts, including Pseudomonas monteilii and Pseudomonas otitis, were also identified. Diverse variants blaNDM-1, blaNDM-5, blaNDM-9, blaNDM-13, and blaNDM-27 were identified. The blaNDM-positive E. coli ST2659 was dominant. blaNDM was found to coexist with mcr-1 (4/51, 7.84%). Horizontal gene transfer plays a vital role in blaNDM transmission within the LPMs. Some blaNDM-harboring clones transfer among animals and the environment through the food chain and close contact. More efforts are needed to curb the transmission trend of blaNDM among humans, animals, and the environment within LPMs.

The transition of surgical simulation training and its learning curve: a bibliometric analysis from 2000 to 2023.

Proficient surgical skills are essential for surgeons, making surgical training an important part of surgical education. The development of technology promotes the diversification of surgical training types. This study analyzes the changes in surgical training patterns from the perspective of bibliometrics, and applies the learning curves as a measure to demonstrate their teaching ability. Related papers were searched in the Web of Science database using the following formula: TS=((training OR simulation) AND (learning curve) AND (surgical)). Two researchers browsed the papers to ensure that the topics of articles were focused on the impact of surgical simulation training on the learning curve. CiteSpace, VOSviewer and R packages were applied to analyze the publication trends, countries, authors, keywords and references of selected articles. Ultimately, 2461 documents were screened and analyzed. The USA is the most productive and influential country in this field. Surgical endoscopy and other interventional techniques publish the most articles, while surgical endoscopy and other interventional techniques is the most cited journal. Aggarwal Rajesh is the most productive and influential author. Keyword and reference analyses reveal that laparoscopic surgery, robotic surgery, virtue reality (VR) and artificial intelligence (AI) were the hotspots in the field. This study provided a global overview of the current state and future trend in the surgical education field. The study surmised the applicability of different surgical simulation types by comparing and analyzing the learning curves, which is helpful for the development of this field.

Delusive chirality and periodic strain pattern in moiré systems

Geometric phase analysis (GPA) is a widely used technique for extracting displacement and strain fields from scanning probe images. Here, we demonstrate that GPA should be implemented with caution when several fundamental lattices contribute to the image, in particular in twisted heterostructures featuring moiré patterns. We find that in this case, GPA is likely to suggest the presence of chiral displacement and periodic strain fields, even if the structure is completely relaxed and without distortions. These delusive fields are subject to change with varying twist angles, which could mislead the interpretation of twist angle-dependent properties.

Factors Affecting Students’ Intention to Donate Cash Waqf: The Mediating Role of Literacy in Indonesia

Purpose - This paper analyzes the factors that influence the intention to donate cash waqf among students with waqf literacy as a mediating variable. Design/Methodology/Approach - Using structural equation modeling (SEM-PLS) to test the proposed model, based on a sample of 80 Islamic Economics students at public and private universities in Bandung Indonesia Findings - The findings show that first, the intention to give cash waqf to students is affected by the level of education and the level of religiosity, while the variable access to information media is not significant; second, the literacy level of cash waqf among students is influenced by the level of education and access to information media, while the level of religiosity is not significant; and thirdly the level of cash waqf literacy does not mediate the influence of education level, level of access to information media and level of religiosity on cash waqf intentions. Practical implications - This study highlights the important role of education level in increasing the level of literacy and cash waqf intentions for students. The implication is that universities are invited to offer innovative patterns of waqf education and training. By providing a clear understanding of cash waqf awareness among college students, this research can assist in designing appropriate literacy educational campaigns. Originality/Value - This study is the first to study factors affecting the students' intention of cash waqf: the mediating role of literacy with literacy as a mediating variable. This research study can assist in designing appropriate educational literacy campaigns.

Diagnostic value of left ventricular layer strain and specific regional strain patterns in cardiac amyloidosis and Fabry disease.

Layer-specific left ventricular (LV) strain alterations have been suggested as a specific finding in Fabry disease (FD). Our study aimed to assess the diagnostic value of layer-specific radial strain (RS) indices compared to the established LV regional strain pattern in cardiac amyloidosis (CA) and FD, i.e. apical sparing and posterolateral strain deficiency (PLSD). We retrospectively analysed the global, subendocardial, subepicardial LV radial strain, the corresponding strain gradient, as well as the regional and global longitudinal strain. The diagnostic accuracy of the diverse LV strain analyses was comparatively assessed using receiver operating characteristic curve and multivariable regression analyses. In 40 FD and 76 CA patients, CA featured more reduced layer strain values [global RS -12.3 (-15.6 to -9.6) in CA vs. -16.7 (-20.0 to -13.6) in FD; P < 0.001; subendocardial RS -22.3 (-27.4 to -15.9) vs. -28.3 (-31.8 to -23.6), P < 0.001; subepicardial RS -6.6 (-8.6 to -4.7) in CA vs. -8.9 (-11.7 to - 6.5) in FD; P < 0.001]. Global radial and longitudinal strain held an area under the curve (AUC) of 0.75 (0.66-0.84) and AUC 0.73 (0.63-0.83). While the apical sparing and PLSD strain pattern showed the highest accuracy as single parameters [AUC 0.87 (0.79-0.95) and 0.81 (0.72-0.89), P < 0.001], the combination of subendocardial RS and the apical sparing pattern featured the highest diagnostic accuracy [AUC 0.92 (0.87-0.97)]. Combining radial strain-derived parameters to the established strain pattern apical sparing and PLSD improve the diagnostic accuracy in the echocardiographic assessment in suspected storage disease.

A Roadmap for Sustainable Community-Engaged Partnerships in Athletic Training and Using a Traveling Athletic Training Camp as an Implementation Strategy.

Improving access to athletic trainers and increasing diversity in the profession have been major goals of the Strategic Alliance, with a particular interest in the secondary school setting. Within many marginalized communities, individuals are often faced with a lack of resources, high rates of poverty, and limited access to health care. This social and economic climate often extends to inequitable athletic training services and patterns of disparate health. Widely used and recognized strategies to cultivate diversity and address health inequities include community-engaged partnerships; however, these approaches are not well implemented across the athletic training discipline. Successful community-engaged partnerships link communities and universities, and they are rooted in intentionality to address intermediate and long-term health equity outcomes. Athletic training professionals and scholars frequently encounter gaps in resources and process-oriented methods to participate in community-engaged efforts that could include a roadmap or pathway to follow. To bridge this gap, our aims were 2-fold: (1) to disseminate a roadmap for building sustainable community-engaged partnerships in athletic training with the intent of promoting diversity, equity, inclusion, and social justice across athletic training education, research, and professional service and (2) to demonstrate how the roadmap can be implemented using a community-based athletic training education camp as an example. Implementation of the athletic training camp using the roadmap took place at secondary schools where community-engaged partnerships have been established throughout a geographic region known as the Alabama Black Belt, a region burdened with poor health outcomes, limited athletic trainer presence, and lower quality of life, exacerbated by racial and socioeconomic inequalities. Implementing this roadmap as a strategy to build sustainable community-engaged partnerships offers an innovative, interactive, and effective approach to addressing community needs by exposing secondary school students to the athletic training profession, advancing equitable athletic training research practices, and upholding and promoting the principles of diversity, equity, inclusion, and social justice in athletic training education.

Cardiac Contractility Drives Cardiac Interoception

Introduction: Many of the receptors for the exteroceptive senses (e.g. vision, hearing, smell, touch, pain, etc.) are known. Interoception allows us to sense threats to our “milieu interior.” The mechanisms by which threats induce a feeling of anxiety manifested by heart palpitations, shortness of breath (dyspnea), and gastric awareness (queasiness) are unknown. Using photoplethysmography, we reported that individual variation in cardiac interoception, i.e. the ability to feel a change in one’s heartbeat without taking one’s pulse, correlated with contrasts in heart rate and stroke volumes among the population. Hypothesis: Because increases in heart rate and stroke volume raise cardiac contractility, we postulated that stretch activated piezo channels in the heart are responsible for cardiac interoception. Methods: Interoceptive awareness was determined with a ten point Likert scale in which volunteers rated their ability to feel their heartbeat. Interoceptive accuracy was measured by asking subjects to count their heartbeats without taking their pulse over four time intervals in random order. Simultaneously, heart rates were surreptitiously recorded with a two lead electrocardiogram (EKG). Cardiac interoceptive accuracy was calculated using the formula 1/4 Σ [1 − (|EKG measured heartbeats − subjectively reported heartbeats|) / EKG measured heartbeats]. We used 2D speckle tracking ultrasound to measure the relationship between cardiac strain patterns, normalized for heart rate (HR) and body surface area (BSA), and cardiac interoception in 12 resting men and women. We also measured gastric interoception by determining the percent increase in the volume of water some subjects could drink after reporting water satiety. Results: Cardiac interoceptive awareness correlated with interoceptive accuracy ( r = 0.73, p = 0.009). Speckle tracking of cardiac chambers with echocardiography demonstrated that global longitudinal left ventricular (LV) strain correlated with total left ventricular preload (atrial reservoir strain, r = 0.83, p = 0.0009) and early diastolic filling (atrial conduit strain, r = 0.88, p = 0.0002). LV strain also correlated with interoceptive awareness ( r = 0.58, p = 0.046). When stratified, individuals with the highest interoceptive accuracy (n = 6), compared to those with the lowest interoceptive accuracy (n = 6), had less LV strain, [values expressed as (percent change/BSA)*HR ± S.E.M., 828 ± 81 vs. 582 ± 47, p = 0.026]. Cardiac interoceptive awareness also correlated with gastric interoception accuracy, (n = 6) r = 0.90, p = 0.015. Conclusions: Changes in the activation of cardiac stretch receptors by cardiac filling and contractility could modify interoceptive awareness in men and women at rest. Contrasts in stretch receptor activation in other contractile organs (e.g. stomach) could explain variation in interoceptive awareness in the population. Henry M. Jackson Foundation for the Advancement of Military Medicine. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Modeling of Fiber Optic Strain Responses to Shear Deformation of Fractures

Identifying distributed strain sensing (DSS) patterns (or signatures), particularly those arising from different hydraulic fracture geometries, has gained significant attention and research effort. Recent works have generated a catalogue of signatures for planar hydraulic fractures in an elastic rock formation. Yet, in numerous cases (e.g., fault motion and some geothermal reservoir stimulation), the main mode of deformation is a shear on a fracture or a network of natural fractures (particularly during low pressure injection/circulation). However, the specific fiber signatures that result from such shear deformation have not been studied. In this study, we use a three-dimensional poroelastic hydraulic fracture simulator to capture the strain signatures resulting from the shear deformation of fractures in various orientations with respect to the monitoring well. Five key cases are examined: one where the fracture strike is perpendicular to the fiber, another with the strike running parallel to the fiber, a third case where the fracture strike is at 45 degrees to the fiber, a fourth case with a strike slip fault perpendicular to the fiber, and a fifth case where fiber is intersecting the fracture. Theoretically and physically meaningful results were obtained in all five cases, which completely differ from the heart-shaped signature of tensile fracture propagation. It was discovered that the strain pattern changes with the shear deformation direction with respect to the fiber. The model is then used to simulate the response of a fracture network at Utah FORGE to injection to assess whether a signature might be expected in response to the planned injection and circulation rates, and, if so, what strain pattern might be expected. The simulation confirms that a strain response can indeed be observed. More importantly, the fiber response that would be detected in the monitoring well would be a combination of strain signatures from dilation and shear deformation of differently oriented natural fractures. The results in this study provide useful insights on the application of fiber to other stimulation and/or circulation scenarios where shear deformation of a fracture or fracture network plays a major role.

Resistance Rates of Mycobacterium tuberculosis Complex Isolates to Primary Anti-tuberculosis Drugs: A 5-Year Retrospective Study

Background/Aim: Tuberculosis remains a major global health problem with a high morbidity and mortality rate, approximately a quarter of the population is infected with tuberculosis. Drug susceptibility testing is an essential tool for identifying and managing drug-resistant tuberculosis. This study was conducted to evaluate the drug susceptibility pattern of Mycobacterium tuberculosis complex strains isolated from a university hospital. Methods: A total of 10900 samples sent to the microbiology laboratory with the suspicion of tuberculosis clinically between January 2018 and January 2022 were analyzed retrospectively. The automated BACTEC MGIT 960 (Becton Dickinson, USA) was used for sample culture and susceptibility testing. The obtained data were statistically analyzed with the Statistical Package for Social Sciences (SPSS version 20). Results: Out of the 154 isolated positive samples, males and females constituted equal parts of the study population (50%). The majority of tuberculosis cases were in the age group 56–75 years (42.2%), Pulmonary TB was detected in (90.3%) of the patients, while extrapulmonary TB cases were observed in (9.7%). As a result of susceptibility studies on positive samples, isoniazid resistance was 5.2%; streptomycin resistance 1.3%; ethambutol resistance was detected at a rate of 0.6%, while no rifampicin resistant sample was found. Both streptomycin and isoniazid resistance were seen together in 1.3% of the samples. Conclusion: A similar resistance pattern of the first-line antituberculosis drugs was observed in other studies conducted in different provinces of Turkey. The absence of multi-drug resistant and extensively drug-resistant tuberculosis in our study indicates that the tuberculosis surveillance program implemented in our region was successful.