Spectrometric Parameters Research Articles

A Thin-foil Proton Recoil spectrometer for DT neutrons using annular silicon detectors

The use of Thin-foil Proton Recoil (TPR) spectrometers to measure neutrons from Deuterium-Tritium (DT) fusion plasma has been studied previously and is a well established technique for neutron spectrometry. The study presented here focuses on the optimisation of the TPR spectrometer configurations consisting of Δ E and E silicon detectors. In addition an investigation of the spectrometer's ability to determine fuel ion temperature and fuel ion density ratio in ITER like DT plasmas has been performed.A Python code was developed for the purpose of calculating detection efficiency and energy resolution as a function of several spectrometer geometrical parameters. An optimisation of detection efficiency for selected values of resolution was performed regarding the geometrical spectrometer parameters using a multi-objective optimisation, a.k.a. Pareto plot analysis. Moreover, the influence of detector segmentation on spectrometer energy resolution and efficiency was investigated. The code also produced response functions for the two selected spectrometer configurations. The SPEC code was used to simulate the spectrometer's performance in determining the fuel ion temperature and fuel ion density ratio nt/nd.The results presented include the selected spectrometer configuration with calculated energy resolution and efficiency. For a selected spectrometer resolution of 5% a maximum efficiency of around 0.003% was achieved. Moreover, the detector segmentation allows for a 20% increase in spectrometer efficiency for an energy resolution of 4.3%. The ITER requirements for a 20% accuracy on the nt/nd ratio determination and 10% on the temperature determination within a 100 ms sampling window can be achieved using a combination of several TPR's of same type, in order to boost efficiency.

Visible wave spectrometric features of scots pine seeds: the basis for designing a rapid analyzer

The study is intended for engineers and small-scale forestry farmers because it offers a simple solution for forest seeds quality improvement by selection before sowing (especially aerial sowing). For coniferous trees seeds VIS spectroscopy is a very important tool that determines the energy of radiation, indicating viability and origin. However, despite many suggestions of potential for practical use, there are no specific recommendations given. Visible diffuse reflectance spectra of single seeds were scanned with spectrometer USB 4000 (Ocean Optics Inc., USA) from 450 to 1000 nm at 1 nm intervals. It is found that in the range from 660 to 715 nm the spectral allocate of seed coat color classes has three non-intersecting characteristic groups differing in the magnitude of the diffuse reflection and forming a possible grading zone. The results of this study are limited to this specific sample and cannot be interpreted for seeds of all coniferous species. There is a need for design of a device for rapid analysis of seeds by spectrometric parameters, which will have a practical application and which will allow a comprehensive sequential studies following the “spectrogram - germination - growth in the nursery-growth in the field” path.

Automated optimization of XCMS parameters for improved peak picking of liquid chromatography-mass spectrometry data using the coefficient of variation and parameter sweeping for untargeted metabolomics.

Accurate peak picking and further processing is a current challenge in the analysis of untargeted metabolomics using liquid chromatography-mass spectrometry (LC-MS) data. The optimization of these processes is crucial to obtain proper results. This study investigated and optimized the detection of peaks by XCMS, a widely used R package for peak picking and processing of high-resolution LC-MS metabolomics data by their coefficient of variation using neat standard solutions of drug like compounds. The obtained results were additionally verified by using fortified pooled plasma samples. Settings of the mass spectrometer were optimized by recommendations in literature to enable a reliable detection of the investigated analytes. XCMS parameters were evaluated using a comprehensive parameter sweeping approach. The optimization steps were statistically evaluated and further visualized after principal component analysis (PCA). Concerning the lower concentrated solution in methanol samples, the optimization of both mass spectrometer and XCMS parameters improved the median coefficient of variation from 24% to 7%, retention time fluctuation from 9.3seconds to 0.54seconds, and fluctuation of the mass to charge ratio (m/z) from m/z 0.00095 to m/z 0.00028. The number of parent compounds and their related species annotated by CAMERA increased from 88 to 113 while the total amount of features decreased from 3282 to 428. Optimized MS settings such as increased resolution led to a higher specificity of peak picking. PCA supported these findings by showing the best clustering of samples after optimization of both mass spectrometer and XCMS parameters. The results implied that peak picking needs to be individually adapted for the experimental set up. Reducing unwanted variation in the data set was most successful after combining high resolving power with strict peak picking settings.

Simple spectral reduction algorithm used for the echelle spectrometer.

The structure of the echelle spectrometer equipped with a prism cross-dispersion element is very complex because of the nonlinear interaction between the prism and the echelle grating, which makes it difficult to extract the wavelength information from the two-dimensional spectrogram. According to the dispersion equations of both the grating and the prism direction, a wavelength calibration model is derived, establishing a relationship between the pixel position and the wavelength. It is found that a calibration process is always needed in repeated experiments due to the slight change of the spectrometer parameters caused by the environment. To solve this problem and improve the accuracy of the reduction, a simple spectral reduction algorithm involving calibration and compensation is proposed, which combines the least-square method and the polynomial fitting method. The algorithm was implemented with LabVIEW software, and the result showed that the absolute error of each wavelength was less than 0.02nm.

Design of experiments for the optimization of electrospray ionization in the LC-MS/MS analysis of ciguatoxins.

Liquid chromatography (LC) coupled to mass spectrometry (MS) is being widely applied as an analytical tool in the field of marine biotoxins both for regulated and for new and emerging compounds. LC-MS/MS recently became the reference method for the control of lipophilic toxins in the European Union, and new methods are being developed and optimized to extend the applicability of this technique to other toxin groups. In this work, conditions for the analysis of ciguatoxins (CTXs) by LC-MS/MS were investigated using standard solutions of CTX1B and CTX3C, which are structurally representative compounds for the rest of the main congeners of Pacific group toxins (P-CTXs). Preliminary studies were carried out for the selection of precursor and product ions used for multiple reaction monitoring. Two transitions based on the chemical structures of CTXs were set up, and mass spectrometer parameters were adjusted for selected reactions monitored. The electrospray ionization source has been carefully optimized through a design of experiments that consisted of a two-level fractional factorial design of resolution IV for the screening of adequate source conditions and of response surface designs for optimization of the main interactions between factors. The statistical approach allowed maximizing the sensitivity on the MS analyzer that provides a good specificity in P-CTX detection, which can be also used for confirmation purposes.

Influence of focusing voltage on sensitivity of quadrupole mass spectrometer

Sensitivity is an important performance parameter of the quadrupole mass spectrometer. The ion source parameters, quadrupole parameters and collector parameters of the quadrupole mass spectrometer all affect the sensitivity of the mass spectrometer, and the focusing voltage of the ion source is a core factor. In this paper, the theoretical analysis of the electromagnetic field of the focusing voltage is carried out. The relationship between the focusing voltage and the sensitivity is simulated by using SIMION software. And the simulation results are verified by experiments. Finally, the proposed method is proposed to improve the sensitivity of the mass spectrometer by adjusting the focusing voltage.

Simultaneous Analysis of 20 Mycotoxins in Grapes and Wines from Hexi Corridor Region (China): Based on a QuEChERS⁻UHPLC⁻MS/MS Method.

The aim of this study is to develop and validate an improved analytical method for the simultaneous quantification of 20 types of mycotoxins in grapes and wines. In this research, the optimization of tandem mass spectrometer (MS/MS) parameter, ultra-high pressure liquid chromatography (UHPLC) separation, and QuEChERS procedure, which includes wine/water ratio, the amount and type of salt, clean-up sorbent, were performed, and the whole separation of mycotoxins was accomplished within 7 min analyzing time. Under optimum conditions, recoveries ranged from 85.6% to 117.8%, while relative standard deviation (RSD) remained between 6.0% and 17.5%. The limit of detection (LOD, 0.06–10 μg/L) and the limit of quantification (LOQ, 0.18–30 μg/L) were lower than those permitted by legislation in food matrices, which demonstrated the high sensitivity and applicability of this efficient method. Finally, 36 grapes and 42 wine samples from the Hexi Corridor region were analyzed. Penicillic acid (PCA), mycophenolic acid (MPA), cyclopiazonic acid (CPA), fumonisin B1 (FB1) and zearalenone (ZEN) were detected in a small number of grape samples with lower concentrations between 0.10 μg/L and 81.26 μg/L. Meanwhile, ochratoxin A (OTA), aflatoxin B2 (AFB2), MPA, CPA, and ZEN were detected in some wine counterparts with concentrations ranged from 0.10 μg/L to 4.62 μg/L. However, the concentrations of the detected mycotoxins were much lower than the maximum legal limits set of other products.

Experimental and theoretical mass spectrometric quantification of diffusion parameters and 3D structural determination of ions of L-tryptophyl-l-tryptophan in electrospray ionization conditions in positive operation mode

The issue of this work focuses on development of quantitative model equations connecting experimental mass spectrometric (MS) parameters, for instance, MS intensity, of analyte peaks with thermodynamic, kinetic and diffusion parameters of the corresponding ions with their 3D molecular and electronic structures. Our more recent contribution to this field has convincingly argued that equation D=1.3194.10−17.A.I2¯−(I¯)2(I−I¯)2, obtained on the base on stochastic dynamics, is applicable to electrospray ionization (ESI), collision induced dissociation (CID) and matrix–assisted laser desorption/ionization (MALDI) mass spectrometric (MS) methods, respectively. It connects MS diffusion parameters of ions with the intensities of their peaks in the MS spectrum. There has been evidenced, that experimental diffusion parameters correspond quantitatively to theoretical quantum chemical diffusion coefficients, obtained on the base on Arrhenius' formalism. If next research effort continues to confirm the validity of the shown above quantitative relation to the behavior of the MS intensity of the analyte, with respect to time, this might imply that the mass spectrometry represents not only an irreplaceable method for quantification in the analytical practice, but it provides exact experimental information about the 3D structure of the analytes. According to the latter line of research, the first aspect of this study is an experimental and theoretical MS quantification of diffusion parameters and 3D structural determination of ions of L-tryptophyl–l–tryptophan (H–Trp–Trp–OH) under ESI(+)–MS conditions. Another issue details experimentally and theoretically the process of molecular cyclization of H–Trp–Trp–OH. This aspect of the chemical reactivity of the dipeptide has provided very important insights into major reaction paths, yielding to permuted oxazolone. Taking a step into a broad application of our model relationship stated above to different areas of science, we should underline that, no doubt, the paper contributes significantly to methodological development of mass spectrometric based methods for 3D structural analysis, exploiting herein a nontrivial case of MS fragment behavior of the peptide H–Trp–Trp–OH under ESI–MS conditions.

Assessment of ethyl sulphate in hair as a marker for alcohol consumption using liquid chromatography-tandem mass spectrometry.

Ethyl glucuronide (EtG) and ethyl sulphate (EtS) are 2 non-oxidative and direct metabolites of ethanol. EtG is known to accumulate in hair and has proved to be a reliable biomarker for detection of chronic alcohol consumption. EtS has been analysed in blood and urine but has never been reported in hair. This article presents the first analytical assay based on liquid chromatography coupled to tandem mass spectrometry for the quantification of EtS in hair. Sample preparation, chromatographic, and mass spectrometric parameters, such as solid-phase extraction, column type, and transitions were optimised. The method was validated according to the guidelines of the European Medicine Agency, fulfilling the requirements for limit of quantification (LOQ), linearity, accuracy, precision, carry-over, matrix effects, and recovery. Linearity ranged from 5 to 500pgmg-1 and the LOQ was achieved at 5pgmg-1 . The novel method was successfully applied to hair samples (n=40) from patients treated for alcohol use disorders. EtS concentrations in hair ranged from 24 to 1776pgmg-1 , while EtG concentrations in hair ranged from 1 to 1149pgmg-1 . Hair concentrations of EtS and EtG were compared to assess the relationship between both biomarkers. There was a significant and positive correlation between EtS and EtG in hair, suggesting that EtS can be used as a biomarker for alcohol consumption. Relatively high basal EtS levels were observed in alcohol-abstinent persons, comparable to what has been reported for EtG. The developed analytical procedure offers an alternative method to prove alcohol consumption using hair analysis.

Solvent composition dependent signal reduction of molecular ions generated from aromatic compounds in (+) atmospheric pressure photoionization mass spectrometry.

The ionization process is essential for successful mass spectrometric (MS) analysis because of its influence on selectivity and sensitivity. In particular, certain solvents reduce the ionization of the analyte, thereby reducing the overall sensitivity in atmospheric pressure photoionization (APPI). Since the sensitivity varies greatly depending on the solvents, a fundamental understanding of the mechanism is required. Standard solutions were analyzed using a (+)-APPI Q Exactive ion trap mass spectrometer (Thermo Scientific). Each solution was infused directly into the APPI source at a flow rate of 100μL/min and the APPI source temperature was 300°C. Other operating mass spectrometric parameters were maintained under the same conditions. Quantum mechanical calculations were carried out using the Gaussian 09 suite program. Density functional theory was used to calculate the reaction enthalpies (∆H) of the reactions between toluene and other solvents. The experimental and theoretical results showed good agreement. The abundances of analyte ions were well correlated with the calculated ∆H values. Therefore, the results strongly support the suggested signal reduction mechanism. In addition, linear correlations between the abundance of toluene and analyte molecular ions were observed, which also supports the suggested mechanism. A solvent composition dependent signal reduction mechanism was suggested and evaluated for the (+)-APPI-MS analysis of polyaromatic hydrocarbons (PAHs) generating mainly molecular ions. Overall, the evidence provided in this work suggests that reactions between solvent cluster(s) and toluene molecular ions are responsible for the observed reductions in signal.

Spectral Response of Few Important Textural Variants of Chromitite and its Potential in Estimating Relative Grades of Chromitite – A Case Study for Chromitite of Nuggihalli Schist Belt, India

We have collected, processed and analysed the reflectance spectra of representative chromitite samples of spot type, clot type and disseminated type textural variants to understand the diagnostic spectral features of each of these samples. We have found that the reflectance spectrum of each textural variant is distinct from the spectra of other variants despite having few common absorption features. Spectral features of chromitite samples are governed by the spectra of two dominant minerals, chromite and chlorite. Spectral features of chromitite at 550 nm and 1100 nm are governed by electronic transition process in Fe 3+ and crystal field effect in Fe 2+ ions present in chromite structure respectively. On the other hand, spectral features at 1400 nm, 1900 nm and 2300 nm are related to the vibration of O–H, H–OH and metal hydroxide bonds in chlorite. Amongst these features, the spectral feature at 1100 nm (due to Fe 2+ in chromite grains) is common to all three major textural varieties of chromitite samples studied here. Electron probe micro analysis (EPMA) data of chromite and chlorite grains of each texture are used to relate the presence and abundance of Fe 2+ (in chromite grains) with absorption feature. Width of the 1100 nm feature has a correlation value 0.95, while depth of the same feature has a correlation value 0.94 with the abundance of chromite mineral estimated using modal analysis of chromite samples. Therefore, spectrometric parameter of 1100 nm spectral feature of chromitite can be used as proxy for estimating modal abundance of chromite in chromitite samples after estimating deposit specific correlation coefficient.

Analysis of microRNA and modified oligonucleotides with the use of ultra high performance liquid chromatography coupled with mass spectrometry

The present study highlights the application of ultra high performance liquid chromatography coupled with mass spectrometry for the selective separation and sensitive quantification of microRNAs and modified phosphorothioate oligonucleotide. The Central Composite Design was used for comprehensive optimization of mass spectrometer parameters (for tandem mass spectrometer and quadrupole–time-of-flight mass spectrometer). Ion pair chromatography was used in order to separate the studied compounds. Furthermore, the optimization of concentration of ion pair reagents in the mobile phase was done with respect to mass spectrometry sensitivity and liquid chromatography separation. The greatest sensitivity for studied compounds was determined for the mixture of 100 mM hexafluoroisopropanol, 5 mM N,N-dimethylbutylamine and methanol. This mobile phase also provided the best separation results in the shortest time for two of the four columns used in the study. Finally, the Hypersil GOLD aQ was selected for routine analysis of microRNA and modified phosphorothioate oligonucleotide in serum samples. These compounds were extracted from the sample with the use of combined liquid-liquid and solid phase extraction. The method developed during the study was then applied for the qualitative and quantitative analysis with limits od quantification equal to 49–63 nM.

Simultaneous determination of neurotoxic shellfish toxins (brevetoxins) in commercial shellfish by liquid chromatography tandem mass spectrometry

An analytical method using liquid chromatography tandem mass spectrometry has been developed for the determination of neurotoxic shellfish poisoning (NSP) toxins (brevetoxins) in shellfish. The tested parameters included extraction solvent, clean-up method, mobile phase and mass spectrometric detection parameters including multiple reaction monitoring (MRM) conditions. The toxins were extracted with 80% methanol in water and cleaned up using Strata-X SPE cartridges. Chromatographic separation of brevetoxins (PbTx-1, PbTx-2 and PbTx-3) was carried out on a C18 column (100 mm × 2.1 mm, 2.6 μm) with gradient elution. The developed method was validated according to the requirements of SANTE 11945/2015. The average recoveries of the brevetoxins ranged from 75.9% to 105.8%, the intra-day precisions were in the range of 0.4–8.6%, the inter-day precisions were in the range of 0.7–6.3% and the limits of quantification (LOQs) were 25 μg/kg for each toxin. The validated method was successfully applied to real shellfish samples, including mussel, oyster and blood cockle. Overall, the method has shown excellent sensitivity and reproducibility, and it could be used as a reference method for the monitoring of individual brevetoxins in shellfish samples.

Multiparameter Optimization for Mineral Mapping Using Hyperspectral Imagery

A multi parameter optimization model is proposed for the mineral mapping of hyperspectral imagery. This model can provide guidance for selecting system parameters when a new sensor is designed or offer performance estimation in mineral mapping with a given imaging system. A multivariate regression analysis is performed to investigate the quantitative relationship between mineral identification capability and imaging spectrometer parameters. The objective function of the proposed optimization model is set to maximize performance with the constraint on system parameters due to user requirements and technology maturity. The number of minerals identified is selected to measure the data usefulness. The parameters of an imaging system include ground sample distance, signal-to-noise ratio, modulation transfer function, and spectral resolution. A set of evaluation experiments is conducted using hyperspectral data collected in Dongtianshan area, Xinjiang, China. The predicted error is less than 15%, and the best mineral mapping can be obtained using the optimized system parameters.

Screening of stationary phase selectivities for global lipid profiling by ultrahigh performance supercritical fluid chromatography

The performance of seven sub-2-μm particle packed columns (2-picolylamine, 2-PIC; charged surface hybrid fluoro-phenyl, CSH-FP; high strength silica C18 SB, HSS-C18; diethylamine, DEA; 1-aminoanthracene, 1-AA; high density diol and ethylene bridged hybrid; BEH) was examined for lipid separation in ultra-high performance supercritical fluid chromatography (UHPSFC) coupled to quadrupole time-of-flight mass spectrometry. Based on the results of the column screening a method for profiling of multiple lipid species from the major lipid classes was developed.Stationary phases containing β-hydroxy amines, i.e. 1-AA, DEA and 2-PIC, yielded strong retention and poor peak shapes of zwitterionic lipids with primary amine groups, such as phosphatidylserines, phosphatidylethanolamines and its lyso forms. The BEH and HSS-C18 columns showed strong retention of polar and nonpolar lipids, respectively. The Diol column retained the majority of major lipid classes and also produced symmetric peaks. In addition, this column also produced the highest resolution within and between major lipid classes. An injection solvent composed of methanol:chloroform (1:2, v:v) and the addition of 20 mM ammonium formate in the mobile phase improved chromatographic separation and mass spectrometry detection in comparison to ammonium acetate or absence of additive. Finally, chromatographic and mass spectrometric parameters were optimized for the Diol column using a design of experiments approach. The separation mechanism on the Diol column depended on the lipid functionality and the length and degree of unsaturation of the acyl groups. The developed method could resolve 18 lipid classes and multiple lipids within each class, from blood serum and brain tissue in 11 min.

Mass spectrometric recommendations for Quan/Qual analysis using liquid-chromatography coupled to quadrupole time-of-flight mass spectrometry

BackgroundHigh-throughput simultaneous quantitative and qualitative (Quan/Qual) analysis is attractive to combine targeted with non-targeted analysis, e.g. in pharmacometabolomics and drug metabolism studies. This study aimed to investigate the possibilities and limitations of high-throughput Quan/Qual analysis by ultra-high performance liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS), to develop a widely applicable Quan/Qual UHPLC-HRMS method and to provide recommendations for Quan/Qual method development. MethodsA widely applicable 4.25-min UHPLC method for small-molecules was used to investigate and optimize mass spectrometric parameters of a Synapt G2S for Quan/Qual analysis. The method was applied on a rat metabolomics study investigating the effect of the fasting state and administration of a dosing vehicle on the rat plasma metabolic profile. ResultsHighly important parameters for high-throughput Quan/Qual analysis were the scan mode and scan rate. A negative correlation was found between the amount of qualitative information that a method can provide and its quantitative performance (accuracy, precision, sensitivity, linear dynamic range). The optimal balance was obtained using the MSE scan mode with a short scan time of 30 ms. This 4.25-min Quan/Qual analysis method enabled quantification with accuracy and precision values ≤ 20% at the lowest quality control (QC) level and ≤15% at higher QC levels for 16 out of 19 tested analytes. It provided both parent m/z values and fragmentation spectra for compound identification with limited loss of chromatographic resolution and it revealed biologically relevant metabolites in its application to the metabolomics study. ConclusionQuan/Qual method development requires balancing between the amount of qualitative data, the quality of the quantitative data and the analysis time. Recommendations are provided for MS resolution, scan mode, scan rate, smoothing and peak integration in Quan/Qual method development and analysis.

X-ray fluorescence determination of Sn, Sb, Pb in lead-based bearing alloys using a solution technique

For the quantitative analysis of the principal elements in lead-antimony-tin alloys, directly X-ray fluorescence (XRF) method using solid metal disks introduces considerable errors due to the microstructure inhomogeneity. To solve this problem, an aqueous solution XRF method is proposed for determining major amounts of Sb, Sn, Pb in lead-based bearing alloys. The alloy samples were dissolved by a mixture of nitric acid and tartaric acid to eliminated the effects of microstructure of these alloys on the XRF analysis. Rh Compton scattering was used as internal standard for Sb and Sn, and Bi was added as internal standard for Pb, to correct for matrix effects, instrumental and operational variations. High-purity lead, antimony and tin were used to prepare synthetic standards. Using these standards, calibration curves were constructed for the three elements after optimizing the spectrometer parameters. The method has been successfully applied to the analysis of lead-based bearing alloys and is more rapid than classical titration methods normally used. The determination results are consistent with certified values or those obtained by titrations.

Simple screening procedure for 72 synthetic cannabinoids in whole blood by liquid chromatography\u2013tandem mass spectrometry

PurposeIn recent years, many synthetic cannabinoids (SCs) have appeared on the drug market. Despite the increasing number of SCs, there are few comprehensive screening methods for their detection in biological specimens. In this context, the purpose of this study was to develop a fast and simple liquid chromatography–tandem mass spectrometry screening procedure for detection and identification of SCs in whole blood.MethodsThe elaborated qualitative screening method allows the simultaneous detection and identification of 72 compounds from different chemical groups: naphthoylindoles, naphthoylindazoles, benzoylindoles, phenylacetylindoles, tetramethylcyclopropylindoles, indole-3-carboxylic acid esters, indole-3-carboxylic acid amides, indazole-3-carboxylic acid amides, and others. Whole-blood samples (0.2 mL) were precipitated with acetonitrile (0.6 mL). The separation was achieved with the gradient of the mobile phase composition (0.1% formic acid in acetonitrile and 0.1% formic acid in water) and the gradient of the flow rate (0.5–0.8 mL/min) in 16 min. Detection of all compounds was based on dynamic multiple reaction monitoring.ResultsMass spectrometer parameters for all compounds were presented. All of the compounds were well-separated by their retention times and/or transitions. The limits of detection (LODs) for 50 compounds were in the range 0.01–0.48 ng/mL.ConclusionsEstimated LODs make this assay suitable for the analysis of biological material. The procedure can be easily expanded for more substances, which is an indispensable advantage in the dynamically developing drug market. It can have wide application in various analytical forensic and clinical laboratories.

ИСПОЛЬЗОВАНИЕ ОТЕЧЕСТВЕННЫХ БИОПРЕПАРАТОВ ДЛЯ ПОВЫШЕНИЯ МЯСНЫХ КАЧЕСТВ БЫЧКОВ

Scientifically substantiated and experimentally proved the feasibility of the zootechnical application of the developed biological products Prevention-N and Prevention N E in technology of beef production for the realization of bio-resource potential meat quality of calves of black-motley breed. Against the background of the use of biopreparations, activation of growth and development of bulls during cultivation, rearing and fattening was established, which led to higher slaughter and meat qualities of carcasses and, as a result, the yield of valuable cuts: spinogrudnogo – by 6.1 and 4.0 kg (P<0.01-0.001), lumbar – by 2.6 and 1.7 kg (P<0.05-0.01) and hip – by 8.6 and 7.1 kg (P<0.001). The quality of meat carcasses was proved by organoleptic, biochemical and spectrometric parameters and, consequently, the safety of the tested preparations. It was found that the implementation of the biological potential of the body of bulls due to the activation of hematopoiesis, cellular and humoral factors nonspecific resistance of biopreparations. The novelty of the obtained data is confirmed by the patents of the Russian Federation for invention № 2602687 and № 2622765 registered in the State register of inventions of the Russian Federation on 26.10.2016 and 19.06.2017, respectively.

Comprehensive evaluation of the crystallogenic properties of human saliva

The purpose of the study was clarification of the possibilities of visual quantitative study of crystalloscopic and teziographic facias of oral fluid of the person and their spectrometric analysis. Material and methods. We studied own and initiated crystallization of the oral liquid of 95 healthy adults without dental pathology (age 24-27 years). crystalloscopic and teziographic facias was evaluated by visuametric and spectrometric parameters. In teziographic test 0.9% sodium chloride solution was used as the basic substance. Results. On the basis of study of own and initiated the crystallogenesis of the oral fluid of healthy adults we stated quantitative visuametric and spectrometric patterns. For the first time we proposed combination of visuametric and instrumental methods in assessment of dehydration biological fluid to verify its results. The obtained norms can be used for further crystalloscopic researches in gastroenterology, stomatology etc.