Paramagnetic Radical Research Articles

Efficient degradation of ciprofloxacin by peroxymonosulfate activated with Co-loaded waste biomass: Efficiency, stability, and mechanism

Waste-derived biochar (BC) loaded with transition metals holds promising potential for remediation of wastewater, owing to the high efficiency and minimal risk of metal leaching. Herein, gold passion fruit shell (PFS) derived biochar loaded with cobalt (Co-PFSBC) was synthesized for removal of ciprofloxacin (CIP) from water by activating peroxymonosulfate (PMS). Both the pyrolysis temperature and the Co doping load significantly influence the physicochemical property of Co-PFSBC, thereby affecting the removal efficiency of CIP. 98.51 % of CIP (10 mg/L) is removed within 120 min under the optimal conditions with 100 mg/L of 4 %Co-PFSBC-400 and 1.5 mM of PMS. In addition, 4 %Co-PFSBC-400 shows excellent catalytic performance for a wide range of refractory organics. Based on the quenching experiment and electron paramagnetic resonance (EPR), both radical and non-radical process (singlet oxygen, 1O2) are involved in the 4 %Co-PFSBC-400/PMS system. The total concentration (2.44 mM) of 1O2 generated in the first 30 min reaction indicates that 1O2 plays a major contribution in the CIP degradation. Moreover, the toxicity of CIP and its degradation intermediates were calculated and a plausible mineralization pathway is proposed according to the intermediates.

Quantitative spatial visualization of X-ray irradiation via redox reaction by dynamic nuclear polarization magnetic resonance imaging

The dose of X-ray irradiation is commonly measured by point assessment with an ionization chamber dosimeter. However, to achieve spatially accurate delivery of X-ray to avoid the exposure to normal tissues, an accurate imaging method for spatially and quantitatively detecting exposure is required. Herein, we present a novel method to visualize X-ray exposure using low-field dynamic nuclear polarization magnetic resonance imaging (DNP-MRI) with nitroxyl radical tempol as the chemical dosimeter. In this system, gel phantoms containing glutathione (GSH) and the paramagnetic tempol radical were used to monitor the deposited X-ray-irradiation via the redox reaction. The tempol radical level was evaluated by DNP-MRI whose signal intensity was linearly correlated with the radical concentration. The radical level in the presence of GSH decreased in proportion to the dose of X-irradiation deposited. In an imaging experiment simulating clinical radiotherapy, we used a clinical linear accelerator with a radiotherapy planning software to confirm the utility of the exposure imaging. The X-ray exposure and its distribution were clearly visualized on the gel phantom image acquired by DNP-MRI. The results were consistent with those specified in the radiotherapy plan where the intensity of the radiation beam was modulated. This exposure estimation will be useful for determining an accurate irradiation field and reducing off-target exposure in clinical settings.

Activation of peroxymonosulfate by boron nitride loaded with Co mixed oxides and boron vacancy for ultrafast removal of drugs in surface water

The mediation of vacancy in catalysts is crucial for the enhancement of oxidant activation. Here the boron nitride loaded with Co mixed oxides (Co2O3-CoO) and boron vacancy (Bv) catalyst (Co/BN-X) was prepared to degrade sulfamethoxazole (SMX) by activating peroxymonosulfate (PMS). Under dark condition, Co/BN-3+PMS system can completely remove SMX in surface water within 15 min, and its removal efficiency constant (0.5154 min−1) was 4.0 and 6.7 times greater than those of Co/BN-2+PMS (0.1284 min−1) and Co/BN-1+PMS (0.0771 min−1), respectively. The system showed excellent performance in different influencing factors and cyclic experiments, and exhibited good practical application potential in secondary effluent. Electron paramagnetic resonance, radical quenching and electrochemical tests certified that singlet oxygen (1O2) was the major active species, followed by •O2–, and SO4•–, further elaborating the activation pathway of PMS in Co/BN-3+PMS system. The density functional theory (DFT) calculations confirmed that CoO and PMS-O2 sites were the main reaction sites, and the existence of Bv reduced the adsorption energy of Co/BN-3 for PMS. This work reveals the synergistic effect between Co oxide sites and Bv on the catalyst surface and offers a potential modification method to accelerate Fenton-like reaction.

Biochar assisted synthesis of α-MnO2 nanowires with superior performance for non-radical activation of peroxymonosulfate

Non-radical activation of peroxymonosulfate (PMS) is a promising water treatment technology for removing refractory organics, but its mechanism is still ambiguous and the development of highly efficient catalyst is still a challenge. Herein, α-MnO2 nanowires were synthesized by a hydrothermal method with the assistance of biochar and their performance for PMS activation was studied. The characterization results show that the addition of biochar had little effect on the crystal phase and valence state of MnO2 but significantly reduced the size of nanowires. These nanowires had mesoporous structure, high surface area and oxidation ability, resulting in their superior performance for removing organic pollutants with PMS activation. The degradation of phenol by α-MnO2-PMS followed first order kinetics with an activation energy of 14.82 kJ mol−1. Electron paramagnetic resonance and radical scavenging experiments demonstrated that this activation process followed the non-radical mechanism. The quenching effect of scavengers led to the misleading role of singlet oxygen. In fact, surface activated PMS (MnO2–PMS*) rather than singlet oxygen was the reactive species. Weak acidic condition was conducive for the formation of MnO2–PMS* complexes. This catalyst exhibited good reusability and high activity for removing various organic pollutants, and the common substances in real water had little effect on its performance, suggesting its potential application in wastewater treatment.

Enhancing degradation of sulfamethoxazole by layered double hydroxide/carbon nanotubes catalyst via synergistic effect of photocatalysis/persulfate activation

A Co3Mn-LDHs and carbon nanotube (Co3Mn-LDHs/CNT) composite catalyst was constructed for permonosulfate (PMS) activation and degrading sulfamethoxazole (SMX) under Vis light irradiation. The introduction of CNTs into Co3Mn-LDHs facilitate the exciton dissociation and carrier migration, and the e− and h+ were readily separated from Co3Mn-LDHs/CNT in the photocatalysis process, which promoted the production rate of reactive oxygen species (ROS), so the Co3Mn-LDHs + Vis + PMS system exhibited better activity with an SMX degradation ratio of 61.25% than those of Co3Mn-LDHs + Vis system (42.30%) and Co3Mn-LDHs + PMS system (48.30%). After 10 cycles, the degradation rate of SMX only decreased by 7.16%, indicating the good reusability of the Co3Mn-LDHs/CNTs catalyst. The results of electron paramagnetic resonance (EPR) analysis and radical quenching experiments demonstrated that that the SO4•− played crucial role for SMX removal in Co3Mn-LDHs/CNTs + Vis + PMS system, and both e− and h+ made an important contribution to activating PMS to produce ROS. Overall, this work provided an excellent catalyst for photo-assisted PMS activation and suggested the activation mechanism for organic pollutant remediation.

Direct observation of photoinduced sequential spin transition in a halogen-bonded hybrid system by complementary ultrafast optical and electron probes

A detailed understanding of the ultrafast dynamics of halogen-bonded materials is desired for designing supramolecular materials and tuning various electronic properties by external stimuli. Here, a prototypical halogen-bonded multifunctional material containing spin crossover (SCO) cations and paramagnetic radical anions is studied as a model system of photo-switchable SCO hybrid systems using ultrafast electron diffraction and two complementary optical spectroscopic techniques. Our results reveal a sequential dynamics from SCO to radical dimer softening, uncovering a key transient intermediate state. In combination with quantum chemistry calculations, we demonstrate the presence of halogen bonds in the low- and high-temperature phases and propose their role during the photoinduced sequential dynamics, underscoring the significance of exploring ultrafast dynamics. Our research highlights the promising utility of halogen bonds in finely tuning functional properties across diverse photoactive multifunctional materials.

Non-radical activation of peroxymonosulfate by modified activated carbon for efficient degradation of oxytetracycline: Mechanisms and applications

The application of heterogeneous metal-free catalysis for non-radical peroxymonosulfate (PMS) oxidation is of great significance because of its low environmental effects and mild oxidant dosage. The purpose of this study was to investigate the activation mechanism of PMS using acid-modified activated carbon and oxytetracycline (OTC) as a representative pollutant. Modified activated carbon (MAC)/PMS system was capable of achieving 100 % degradation efficiency of OTC within 60 min, with an observed rate constant (kobs) of 0.0414 min−1. The degradation of OTC is dominated by non-radical oxidation pathways involved in mediated singlet oxygen (1O2) and electron-transfer process through electron paramagnetic resonance (EPR) and radicals quenching studies. The activation of PMS is attributed to the structural defects of MAC, persistent free radicals, and oxygen functional groups such as C-OOH. Moreover, the MAC/PMS system consistently demonstrates high and reliable contaminant removal in various water matrices. A continuous-flow device utilizing MAC shows excellent performance in purifying micro-polluted water. Additionally, the degradation pathways of pollutants and changes in toxicity during the degradation process were identified through density functional theory (DFT) calculations, liquid chromatography-mass spectrometry (LC-MS), and toxicological analysis. Finally, the cultivation of green beans, peas, and wheat was found to significantly decrease the toxicity of contaminated water through degradation. This study proposes a low-cost method to enhance the activation pathway of non-radical PMS by utilizing modified activated carbon materials for pollutant remediation.

A urea-based approach to synthesize single atom iron catalysts for catalyzing peroxymonosulfate to degrade antibiotics

Understanding the role and catalytic mechanism of single atomic catalysts (SACs) in activating peroxymonosulfate (PMS) and subsequently eliminating antibiotics in environmental water is crucial for designing more efficient catalysts. Despite C-N-metal SACs exhibiting excellent performance, their application is constrained by the intricate N-doped pretreatment process and the high cost of carrier materials. To handle these challenges effectively, a new approach, by using molten urea as both solvent and nitrogen source and polyethylene glycol as carbon source, was employed to synthesize the u-Fex-N-C (x = 1, 2, 3) catalysts. In which, u-Fe1-N-C had achieved an impressive 92 % degradation ratio for oxytetracycline (OTC) and remains unaffected by pH in the range of 3–11. It further exhibited catalytic degradation activity towards a broad spectrum of environmental pollutants, including sulfamethoxazole (SMZ), naproxen (NPX), ofloxacin (OFL), rhodamine B (RhB), and bisphenol A (BPA), and the degradation efficiencies reached 67 %, 83 %, 77 %, 99 %, and 93 %, respectively. According to a series of the characterization, the prepared u-Fe1-N-C possessed a unique FeN4 structure. Electron paramagnetic resonance (EPR) and radical quenching experiments proved that 1O2 and Fe (Ⅳ) played a key role in the current reaction system. In-situ Raman and electrically coupled oxidation systems also proved that the non-radical degradation pathway involving electron transfer in the u-Fe1-N-C/PMS system was the primary way for activating PMS. The degradation efficiency of OTC from real water samples still reached 84 %. This work provides a simple and cost-effective strategy for synthesizing SACs, offering promising applied prospects in the field of water treatment and environmental remediation.

Hollow CoP/carbon as an efficient catalyst for the peroxymonosulfate activation derived from phytic acid assisted metal-organic framework

The catalyst's composition and rationally designed structure is significantly interlinked with its performance for wastewater remediation. Here, a novel hollow cobalt phosphides/carbon (HCoP/C) as an efficient catalyst for activating peroxymonosulfate (PMS) was prepared. The ZIF-67 was synthesized first, followed by phytic acid (PA) etching and then heat treatment was used to get HCoP/C. The PA was used as an etching agent and a source of phosphorus to prepare HCoP/C. To analyze catalytic performance, another solid cobalt phosphides/carbon (SCoP/C) catalyst was prepared for comparison. In contrast to SCoP/C, the HCoP/C exhibited higher catalytic efficiency when used to activate PMS to degrade Bisphenol A (BPA). The results showed that about 98 % of targeted pollutant BPA was removed from the system in 6 min with a rate constant of 0.78 min−1, which was 4 times higher than the solid structure catalyst. The higher catalytic performance of HCoP/C is attributed to its hollow structure. In the study, other parameters such as BPA concentration, temperature, pH, and different catalyst amount were also tested. Moreover, the electron paramagnetic resonance (EPR) and radical quenching analysis confirmed that sulfate radicals were dominant in the HCoP/C/PMS system.

Efficient peroxymonosulfate activation by nanoscale zerovalent iron for removal of sulfadiazine and sulfadiazine resistance bacteria: Sulfidated modification or not

Whether it’s necessary to extra chemical synthesis steps to modify nZVI in peroxymonosulfate (PMS) activation process are worth to further investigation. The 56 mg/L nZVI/153.65 mg/L PMS and 56 mg/L sulfidated nZVI (S-nZVI) (S/Fe molar ratio = 1:5)/153.65 mg/L PMS) processes could effectively attain 97.7% (with kobs of 3.7817 min−1) and 97.0% (with kobs of 3.4966 min−1) of the degradation of 20 mg/L sulfadiazine (SDZ) in 1 min, respectively. The nZVI/PMS system could quickly achieve 85.5% degradation of 20 mg/L SDZ in 1 min and effectively inactivate 99.99% of coexisting Pseudomonas. HLS-6 (5.81-log) in 30 min. Electron paramagnetic resonance tests and radical quenching experiments determined SO4•−, HO•, 1O2 and O2•− were responsible for SDZ degradation. The nZVI/PMS system could still achieve the satisfactory degradation efficiency of SDZ under the influence of humic acid (exceeded 96.1%), common anions (exceeded 67.3%), synthetic wastewater effluent (exceeded 90.7%) and real wastewater effluent (exceeded 78.7%). The high degradation efficiency of tetracycline (exceeded 98.9%) and five common disinfectants (exceeded 96.3%) confirmed the applicability of the two systems for pollutants removal. It’s no necessary to extra chemical synthesis steps to modify nZVI for PMS activation to remove both chemical and biological pollutants.

Oxygen-enriched vacancy spinel Mn-Co oxides by deep thermal reduction for enhanced antibiotics degradation efficiency

In this research work, flower-like MnCo2O4.5 was synthesized by introducing a short-chain surfactant as a surface-active agent. This structure was utilized as a precursor, and a gas atmosphere pyrolytic deep reduction strategy was employed to construct a sea urchin-like catalyst (DTR-Vo-MCO) with abundant oxygen vacancies. A series of characterizations were conducted to reveal the catalyst's microstructure and crystal phase composition. DTR-Vo-MCO achieved more than 90% degradation of OTC within 10 min, and the efficiency was 1.5 times that of Vp-MCO. The substantial presence of oxygen defects on the DTR-Vo-MCO surface expedited charge transfer processes, resulting in a remarkable enhancement of PMS activation efficiency. Electron paramagnetic resonance spectroscopy (EPR) and radical quenching experiments indicated that the contributions of radicals to oxytetracycline (OTC) degradation were in the order of 1O2 > SO4•– > O2•– > •OH. 1O2 and SO4•– played dominant roles in OTC degradation. The possible oxytetracycline degradation pathways were proposed based on LC-MS analysis. In summary, this work presents an efficient method for a highly oxygen-deficient catalyst, expanding the application of binary transition metal materials in environmental remediation.

Magnetic CoFe alloy@N-CNTs as fenton-like activator for the efficient abatement of imidacloprid: Kinetics, transformation mechanisms, and toxicity assessment

A magnetic bimetal-carbon nanohybrid, CoFe alloy@N-CNTs was designed to activate peroxydisulfate (PDS) for the degradation of imidacloprid (IMI). Under optimal conditions, 5 mg/L of IMI were completely removed within 6 h with a mineralization degree of 42.9 %. Moreover, a high rate of IMI removal was observed over a wide pH range and in the presence of different anions and humic acids. Stability tests conducted over five cycles and material characterizations confirmed the stability of CoFe alloy@N-CNTs. Furthermore, radical and non-radical oxidation contributed to the degradation process based on the results of quenching experiments, electron paramagnetic resonance (EPR), radical quantification and X-ray photoelectron spectroscopy (XPS). Hydroxylation was identified as the main degradation pathway through mass spectrometry observation, frontier electron densities (FEDs) theory calculations, fluorescence intensities and mineralization degree. Additionally, the predicted ecotoxicity of IMI and its intermediates towards three aquatic organisms using ECOSAR software was not harmful or low toxic, verifying the potential application of this technique from an ecological risk perspective. This study provides valuable insights for designing metal–carbon nanohybrids as efficient Fenton-like catalyst in water treatment.

A novel sugarcane residue-derived bimetallic Fe/Mn-biochar composite for activation of peroxymonosulfate in advanced oxidation process removal of azo dye: Degradation behavior and mechanism

Metal-biochar composites with varying Fe/Mn ratios were synthesized for activating peroxymonosulfate (PMS) oxidative degradation of azo dye Orange G (OG) were investigated. The 1Fe2Mn-BC composite based on 1:2 molar ratio impregnation of Fe/Mn salt concentrations of efficient sugarcane harvest residue showed the best catalytic performance under aqueous pH 3–10 and had the least impacts from coexistence of inorganic anions and humic acids. The OG degradation by 1Fe2Mn-BC/PMS system optimized at catalyst:oxidant:pollutant ratio of 1:1.25:0.5 on g/L basis was found to follow pseudo-first-order kinetic and achieved 100 % degradation efficiency within 30 min. Apparent activation energy for OG degradation by 1Fe2Mn-BC/PMS was 16.91 kJ mol−1, and increasing reaction temperature from 293 to 313 K enhanced degradation rate constant by 49 %. Electron paramagnetic resonance (EPR) and radical quenching experiments revealed dominant non-radical 1O2 and radical O2− pathways in the OG oxidation removal by the 1Fe2Mn-BC/PMS system, although SO4− and OH radicals also participated. XPS spectra of 1Fe2Mn-BC before and after OG degradation indicated dominant surface composition of Fe(III) and Mn(II) valence states over other FeMn-biochar composites. The novel optimized one-step pyrolysis Fe/Mn bimetal biochar composite provides an efficient PMS activation for advanced oxidation process (AOP) treating of azo dyes in wastewater decontamination.

Activation of Z-scheme heterojunction of peroxymonosulfate for augmented visible-light-induced photocatalytic decomposition of tetracycline and enhanced antimicrobial efficacy with O-doped g-C3N4@Co3O4

The widespread use of graphitic carbon nitride (CN) for environmental pollutant removal has been hampered by intrinsic limitations, notably facile electron-hole recombination and a limited surface area. This work successfully synthesized OCN/Co3O4 composite materials. Furthermore, the implementation of a peroxymonosulfate (PMS) reaction system remarkably enhanced the material’s capability to degrade Escherichia coli (E. coli) and tetracycline hydrochloride (TC). Compared with CN, the composite material possessed a notably larger specific surface area, affording it a profusion of reactive sites. Additionally, the replacement of carbon atoms with oxygen atoms in the original CN structure profoundly altered its electronic configuration. The characterization through UV–Vis diffuse reflectance spectra, photoluminescence, transient photocurrent responses, and electrochemical impedance spectroscopy also confirmed the excellent optical and electrochemical attributes of OCN/Co3O4. Experimental results illustrated OCN/Co3O4/Vis/PMS completely degraded E. coli at an initial concentration of 1 × 107 CFU mL−1 within 1 h and reduced TC with the adding concentration of 10 mg/L by 99.2% in only 20 min. An analysis was conducted on reactive oxygen species (ROS) using electron paramagnetic resonance spectroscopy and radical quenching experiments. The test results demonstrated the generation of ROS including •O2−, •OH, h+, and SO4•− within the reaction system. This work explored the degradation mechanism in the OCN/Co3O4/Vis/PMS system, providing innovative insights for the design of eco-friendly, efficient catalysts as well as the enhancement of TC and E. coli degradation through the synergistic activation of photocatalytic PMS.

In-situ heterogeneous electro-Fenton based on B, Fe-modified carbon felts for phosphorus nutrients treatment in dredged sediment

A novelty in-situ heterogeneous electro-Fenton based on boron-modified and co-modified carbon felts (CF) such as B-CF, B, Fe-CF and B, Co-CF was employed for phosphorus nutrients treatment in dredged sediment. During which B, Fe-CF (1:1) was the optimal cathode for phosphorus nutrients and organic matter treatment with organic phosphorus (OP) and non-apatite inorganic phosphorus (Fe-P) conversion rates of 26.84% and 63.21%, respectively, since the generated H2O2 on bifunctional cathode could produce ·OH with material surface reduced Fe(II). Characterized by SEM, XRD and XPS, B and Fe were successfully loaded on the electrode with elemental percentages of 0.99% and 4.40%, while B2O3 and Fe2O3 were detected. The operating parameters like initial pH and current density were explored. B, Fe-CF had a high stability and outstanding catalytic capacity for 5 cycles of phosphorus nutrient treatment. The possible mechanism was also proposed based on the electron paramagnetic resonance (EPR) and radical quenching experiment, which demonstrated that ⋅OH and SO4·- mainly contributed to phosphorus nutrients treatment. In this work, a highly efficient and stable carbon material was successfully prepared to treat refractory pollutants in dredging sediment, which provides new ideas and methods for subsequent resource utilization.

Interrogating the CISS effect in chiral and paramagnetic organic radicals: the impact of the molecular spin over the total spin polarization

Enantioenriched PTM radical self-assembled monolayers on gold/nickel are prepared. Their spin filtering ability is not observed experimentally. The lack of CISS observation is explained by DFT quantum transport calculations.

The synergistic effect between Golden root and Cannabis Sativa L. Determination of the antioxidant activity of the extracts

The study aimed to compare the chemical composition and in vitro antioxidant activity of an extract obtained from Cannabis sativa L and golden root (Rhodiola rosea L), which are both used as natural anxiolytics. The phytochemical profile (HPLC - DAD analysis), total polyphenol content (TPC), and antioxidant capacity (AOC) of the combination of Rhodiola rosea extract and Cannabis sativa oil were investigated to study their effects. To determine the DPPH radicalscavenging activity, an electron paramagnetic radical spectrometer (EPR-X-band) was used as a promising technique. The results indicated that the new extract derived from the combination of Rhodiola rosea and Cannabis sativa was richer in phenolic compounds and exhibited higher antioxidant activity. These findings provide valuable insights for potential in vivo studies that involve the simultaneous use of the two plants.

CoFe2O4/WS2 as a highly active heterogeneous catalyst for the efficient degradation of sulfathiazole by activation of peroxymonosulfate

In this study, MFe2O4/WS2 (M = Co, Cu, Mn, Ni) catalysts were synthesized to activate peroxymonosulfate (PMS) for sulfathiazole (STZ) degradation by decorating with WS2 on the surface of a series of spinel-type transition metal oxides. It was found that the CoFe2O4/WS2/PMS exhibited a greater ability to degrade sulfathiazole (STZ) than other systems. More specifically, the catalyst facilitated Fe3+/Fe2+ recycling to activate PMS efficiently and maintained synergies between CoFe2O4 and WS2 to degrade pollutants. The CoFe2O4/WS2 dosage, PMS concentration, solution pH, inorganic anions, and natural organic matter, which could affect the catalytic efficiency, were inspected. The experimental results manifested that the catalyst displayed outstanding performance in the pH range from 5 to 9. Besides, electron paramagnetic resonance spectroscopy and radical quenching experiments confirmed the presence of HO•, SO4•−, O2•−, and 1O2. Based on detected intermediates, the plausible degradation pathway of STZ was proposed.

Streamlined fabrication of AuPtRh trimetallic nanoparticles supported on Ti3C2MXene for enhanced photocatalytic activity in cephalosporins degradation

The escalating prevalence of cephalosporin antibiotics in wastewater poses a serious threat to public health and environmental balance. Thus, it is crucial to develop effective methods for removing cephalosporin antibiotics from water sources. Herein, we propose the use of AuPtRh trimetallic nanoparticles supported on Ti3C2MXene as a photocatalyst for the degradation of cephalosporin antibiotics. Initially, AuPtRh nanoparticles were uniformly grown onto Ti3C2MXene sheets using one-step reduction technique. The prepared AuPtRh/Ti3C2MXene exhibited a complete degradation of cefixime and ceftriaxone sodium, while an impressive degradation efficiency of 91.58 % for cephalexin was achieved after 60 min of exposure to visible light, surpassing the performance of its individual AuPtRh nanoparticles and Ti3C2MXene. The enhanced photoactivity of AuPtRh/Ti3C2MXene was resulted from improved light absorption capacity and efficient generation, separation, and transfer of charge carriers driven by the formation of heterojunction between AuPtRh and Ti3C2MXene. Electron paramagnetic resonance and radicals trapping experiments results revealed that •O2– and h+ are the principal reactive species governing the degradation of cephalosporins. The photocatalyst exhibited excellent stability and could be reused four times without significant loss in efficiency. Our study highlights the potential of MXene composites for environmental remediation, offering insights into designing sustainable AuPtRh/Ti3C2MXene photocatalyst for water pollutant degradation.

Detecting adulteration of honey with sugar syrup using EPR spectroscopy: A feasibility study

The food adulteration is a common practice nowadays. Honey is an important food product that is getting adulterated. The substances most often incorporated into honey are sugar syrups as they are readily available. The added syrups lower the quality of the honey. Since the sugars have the same chemical composition, as the sugars in honey, there may not be many changes in the chemical parameters of pure honey and honey adulterated with sugar syrup. In this work, we are analyzing the feasibility of using Electron Paramagnetic Resonance (EPR) spectra of the samples for detection of honey adulteration with sugar syrup. The EPR spectrums of the samples are obtained by incorporating a paramagnetic nitroxide radical.