Paired Analysis Research Articles

A Study of a Media Literacy-Facilitated Class Model to Improve Korean Undergraduates’ Critical Thinking and English Writing: Focusing on Modern Satiric Grotesque and Metaphor*

This study explored a media literacy-facilitated learning model to activate EFL undergraduates’ writing performance and critical thinking. While cultivating learners’ media literacy ability, it designed a learning environment to stimulate critical thinking through metaphors and grotesque pictures. The participants (<i>N</i> = 57) tried to find each metaphor while seeing grotesque images from the movies of <i>Parasite</i> (Bong, 2019) and <i>Joker</i> (Philips, 2019). In the process, they learned how to respect peers’ ideas through similarities and differences. They could extend their existing knowledge when allowed to review the social or economic issues that the grotesque images revealed. They reproduced short sentences online in the group Kakao talk room when given specific words aligned with the pictures. When planning their writing, selecting the appropriate words, and correcting their sentences, they reorganized their thoughts and produced a theme-based essay. These activities affected their language performance as the learners could acquire long-term retention about vocabulary use. A paired t-test and One-way ANOVA analysis showed their language proficiency improvement. Their writing shows specific forms (to <i>verb</i>, and <i>to noun</i>) more frequently to express the metaphors of a <i>mask</i>. Accordingly, this model activated critical thinking through metaphor and the satirical grotesque.

The Effect of Using Powerpoint Media on The Ability to Solve Mathematical Problems of Flat Shapes

This study aims to determine the effect of using Powerpoint media on the ability of fourth-grade students of SDN 2 Singocandi to solve mathematical problems based on Polya's theory. Based on the results of tests and interviews, it appears that student's ability to solve mathematical problems is still lacking. This research uses quantitative methods with a survey research type. The population in this study were fourth-grade students of SDN 2 Singocandi. The sample taken in this study was all students totaling 20 students. The sampling technique was carried out using a saturated sampling technique which was. Data collection techniques included written tests, interviews, and documentation. This research design is pre-test and post-test. The analysis technique used is the paired sample t-test analysis technique. The results showed that the use of PowerPoint learning media affected the skills of solving mathematical problems on flat material, which was categorized as (1) Students in the high category who can solve problems understand, plan, implement, and check answers by Polya's steps. (2) Students in the medium category can understand the problem, make a solution plan, and implement the plan, but do not check the answer. (3) Students in the low category were unable to solve the problem according to the steps of Polya's theory.

Characterization of longitudinal nasopharyngeal microbiome patterns in maternally HIV-exposed Zambian infants.

Previous studies of infants born to HIV-positive mothers have linked HIV exposure to poor outcomes from gastrointestinal and respiratory illnesses, and to overall increased mortality rates. The mechanism behind this is unknown, but it is possible that differences in the nasopharyngeal (NP) microbiome between infants who are HIV-unexposed or HIV-exposed could play a role in perpetuating some outcomes. We conducted a longitudinal analysis of 170 NP swabs of healthy infants who are HIV-exposed (n=10) infants and their HIV(+) mothers, and infants who are HIV-unexposed, uninfected (HUU; n=10) .and their HIV(-) mothers. These swabs were identified from a sample library collected in Lusaka, Zambia between 2015 and 2016. Using 16S rRNA gene sequencing, we characterized the maturation of the microbiome over the first 14 weeks of life to determine what quantifiable differences exist between HIV-exposed and HUU infants, and what patterns are reflected in the mothers' NP microbiomes. In both HIV-exposed and HUU infants, Staphylococcus and Corynebacterium began as primary colonizers of the NP microbiome but were in time replaced by Dolosigranulum, Streptococcus, Moraxella and Haemophilus. When evaluating the interaction between HIV exposure status and time of sampling among infants, the microbe Staphylococcus haemolyticus showed a distinctive high association with HIV exposure at birth. When comparing infants to their mothers with paired analyses, HIV-exposed infants' NP microbiome composition was only slightly different from their HIV(+) mothers at birth or 14 weeks, including in their carriage of S. pneumoniae, H. influenzae, and S. haemolyticus. Our analyses indicate that the HIV-exposed infants in our study exhibit subtle differences in the NP microbial composition throughout the sampling interval. Given our results and the sampling limitations of our study, we believe that further research must be conducted in order to confidently understand the relationship between HIV exposure and infants' NP microbiomes.

The effect of rehabilitation measures on the severity of endotoxicosis and its relationship with changes in hemorheology in acute poisoning

Introduction. The use of multidimensional statistical analysis methods (factorial and cluster methods) to study the pathogenesis and sanogenesis of acute poisoning (AP) with psychopharmacological agents (pPPhA), neurotoxicants (pNT) and corrosive substances (pCS) did not allow us to judge the presence of a direct connection between the hemorhealogy system (HR). We received a positive answer to this question earlier with the help of pair correlation analysis (PCA). New data on the sanogenetic effect of the applied methods of rehabilitation treatment in terms of changes in the indicators of HR and endotoxicosis (ET) and the relationship between them are also interesting. The purpose of the work is to assess the initial state of the indicators of HR and ET and the effect on them of a combination of medicinal and non–medicinal products used as part of rehabilitation measures for AP. Material and methods. Paired correlation analysis of the block structure of the obtained correlation matrices was applied to 52 patients with severe pPPhA, pNT and pCS. This analysis established the relationship between two indicators – both before the start of rehabilitation measures (the use of ethylmethylhydroxypyridine succinate, laser hemotherapy, hyperbaric oxygenation and mesodiencephalic modulation in accordance with the type of AP), and after their completion, before the discharge of patients from the hospital. Results. Statistically significant correlations were established between the indicators of HR and ET both before the start of treatment (0.44–0.94, without sign) and after its completion (0.49–0.99, without sign), which allowed us to confirm at a higher level a direct link between the development of HR and ET disorders, as well as to draw a conclusion about the sanogenetic effect of rehabilitation treatment. Limitations. The study has no limitations for further use of this methodological approach. Conclusion. The obtained data can serve as a justification for the introduction into practice of the proposed methods of mathematical analysis of the results of treatment measures.

Objective Comparison of the First-Person-View Live Streaming Method Versus Face-to-Face Teaching Method in Improving Wound Suturing Skills for Skin Closure in Surgical Clerkship Students: Randomized Controlled Trial.

The use of digital online teaching media in improving the surgical skills of medical students is indispensable, yet it is still not widely explored objectively. The first-person-view online teaching method may be more effective as it provides more realism to surgical clerkship students in achieving basic surgical skills. This study aims to objectively assess the effectiveness of the first-person-view live streaming (LS) method using a GoPro camera compared to the standard face-to-face (FTF) teaching method in improving simple wound suturing skills in surgical clerkship students. A prospective, parallel, nonblinded, single-center, randomized controlled trial was performed. Between January and April 2023, clerkship students of the Department of Surgery, Pelita Harapan University, were randomly selected and recruited into either the LS or FTF teaching method for simple interrupted suturing skills. All the participants were assessed objectively before and 1 week after training, using the direct observational procedural skills (DOPS) method. DOPS results and poststudy questionnaires were analyzed. A total of 74 students were included in this study, with 37 (50%) participants in each group. Paired analysis of each participant's pre-experiment and postexperiment DOPS scores revealed that the LS method's outcome is comparable to the FTF method's outcome (LS: mean 27.5, SD 20.6 vs FTF: mean 24.4, SD 16.7; P=.48) in improving the students' surgical skills. First-person-view LS training sessions could enhance students' ability to master simple procedural skills such as simple wound suturing and has comparable results to the current FTF teaching method. Teaching a practical skill using the LS method also gives more confidence for the participants to perform the procedure independently. Other advantages of the LS method, such as the ability to study from outside the sterile environment, are also promising. We recommend improvements in the audiovisual quality of the camera and a stable internet connection before performing the LS teaching method.

450: Lateral pelvic lymph nodal boost in locally advanced rectal cancer: A matched pair analysis

450: Lateral pelvic lymph nodal boost in locally advanced rectal cancer: A matched pair analysis

Detection of Congenital Toxoplasmosis and Cytomegalovirus Infections Using Paired Sample Serodiagnosis from Suspected Cases at a Tertiary Teaching Hospital in Malaysia.

Congenital toxoplasmosis and congenital cytomegalovirus (cCMV) infections are noteworthy in Malaysia and can cause serious health problems in neonates. The prompt and effective detection and treatment related to both illnesses may mitigate the possibility of adverse consequences from both infections. A total of 219 neonates with suspected clinical indications of congenital toxoplasmosis and/or cCMV infections from January 2022 to December 2022 were enrolled. The first samples for IgM and IgG antibodies were screened by electrochemiluminescence immunoassay. For positive results indicative of congenital toxoplasmosis and cCMV infections, second serum samples were requested and tested within a period of 2-4 weeks after testing the first sample. From the 219 first serum samples, the overall seroprevalence of congenital toxoplasmosis antibodies in suspected cases was 53%; meanwhile, the overall seroprevalence of cCMV in the suspected cases was 98.6%. The results of the paired serum sample collected for investigating congenital toxoplasmosis cases revealed that 47% of the cases presented no serological evidence of exposure while the remaining 53% of cases might have acquired passive immunity from the mother. For cCMV, the number of cases with no serological evidence of exposure was 1.4%, whereas acute infection was 1.8% and possible passive immunity from the mother represented 96.8%. This study found a high seroprevalence of congenital toxoplasmosis and cCMV infections, probably because they are suspected cases. This study also indicates that using paired sample analysis in the categorisation of cases can aid in accurate diagnosis and more effective treatment.

Use of video-recordings of site-based interviews for quality assurance in a study of subjects with schizophrenia and persistent negative symptoms

Site-independent ratings derived from audio-digital recordings of site-based interviews are often used for quality assurance monitoring to affirm ratings reliability in CNS clinical trials. The present study of subjects with schizophrenia and persistent negative symptoms used video instead of audio recordings of site-based interviews and thereby facilitated visual observation of the subject by the remote rater. “Paired” site-independent scores of the Positive and Negative Syndrome Scale (PANSS) and Brief Negative Symptom Scale (BNSS) were obtained from video-recordings of site-based interviews.The intraclass correlation between site-based and paired site-independent ratings was r = 0.839 for the total PANSS scores (n = 1006) and r = 0.871 for the total BNSS scores (n = 892); <5 % of paired scores deviated outside the acceptable confidence intervals. Ratings “outliers” were identified and remediated.We examined the pattern of paired scoring deviations for the BNSS, total PANSS, PANSS symptom subscales, and the Marder negative symptom factor. Each metric revealed a bidirectional pattern of scoring deviations such that mean site-based ratings were higher than site-independent ratings when symptom severity was high but lower than site-independent ratings when symptom severity was low.The pattern of bidirectional paired scoring deviations observed in this analysis has previously been noted in paired ratings analyses of subjects experiencing an acute exacerbation of psychosis in schizophrenia and major depressive disorder as well. The bidirectional pattern may reflect inherent differences between live ratings and remotely scored recorded ratings.This analysis affirms the utility of video-recordings of site-based ratings for surveillance in trials with subjects with schizophrenia and persistent negative symptoms.

Comparison of the SBAR method and modified handover model on handover quality and nurse perception in the emergency department: a quasi-experimental study

BackgroundEffective information transfer during nursing shift handover is a crucial component of safe care in the emergency department (ED). Examining nursing handover models shows that they are frequently associated with errors. Disadvantages of the SBAR handover model include uncertainty of nursing staff regarding transfer of responsibility and non-confidentiality of patient information. To increase reliability of handover, written forms and templates can be used in addition to oral handover by the bedside.AimsThe purpose of this study is to compare the ‘Situation, Background, Assessment, Recommendation (SBAR) method and modified handover model on the handover quality and nurse perception of shift handover in the ED.MethodsThis research was designed as a semi-experimental study, with census survey method used for sampling. In order to collect data, Nurse Perception of Hanover Questionnaire (NPHQ) and Handover Quality Rating Tool (HQRT) were used after translating and confirming validity and reliability used to direct/collect data. A total of 31 nurses working in the ED received training on the modified shift handover model in a one-hour theory session and three hands-on bedside training sessions. This model was implemented by the nurses for one month. Data was analyzed with SPSS (version 26) using paired t-tests and analysis of covariance.ResultsResults indicated significant difference between the modified handover model and SBAR in components of information transfer (P < 0.001), shared understanding (P < 0.001), working atmosphere (P = 0.004), handover quality (P < 0.001), and nurse perception of handover (P < 0.001). The univariate covariance test did not show demographic variables to be significantly correlated with handover perception or handover quality in SBAR and modified methods (P > 0.05).ConclusionsThe results of this study can be presented to nursing managers as a guide in improving the quality of nursing care via implementing and applying the modified handover model in the nursing handover. The resistance of nurses against executing a new handover method was one of the limitations of the research, which was resolved by explanation of the plan and goals, as well as the cooperation of the hospital matron, and the ward supervisor. It is suggested to carry out a similar investigation in other hospital departments and contrast the outcomes with those obtained in the current study.

Comparison of physiological and clinical reactions to COVID-19 and influenza vaccination

BackgroundPublic reluctance to receive COVID-19 vaccination is associated with safety concerns. By contrast, the seasonal influenza vaccine has been administered for decades with a solid safety record and a high level of public acceptance. We compare the safety profile of the BNT162b2 COVID-19 booster vaccine to that of the seasonal influenza vaccine.MethodsWe study a prospective cohort of 5079 participants in Israel and a retrospective cohort of 250,000 members of MHS selected randomly. We examine reactions to BNT162b2 mRNA COVID-19 booster and to influenza vaccinations. All prospective cohort participants wore a smartwatch and completed a daily digital questionnaire. We compare pre-vaccination and post-vaccination smartwatch heart-rate data, and a stress measure based on heart-rate variability. We also examine adverse events from electronic health records.ResultsIn the prospective cohort, 1905 participants receive the COVID-19 booster vaccine; 899 receive influenza vaccination. Focusing on those who receive both vaccines yields a total of 689 participants in the prospective cohort and 31,297 members in the retrospective cohort. Individuals reporting a more severe reaction after influenza vaccination tend to likewise report a more severe reaction after COVID-19 vaccination. In paired analysis, the increase in both heart rate and stress measure for each participant is higher for COVID-19 than for influenza in the first 2 days after vaccination. No elevated risk of hospitalization due to adverse events is found following either vaccine. Except for Bell’s palsy after influenza vaccination, no elevated risk of adverse events is found.ConclusionsThe more pronounced side effects after COVID-19 vaccination may explain the greater concern associated with it. Nevertheless, our comprehensive analysis supports the safety profile of both vaccines.

FUSION: A web-based application for in-depth exploration of multi-omics data with brightfield histology.

Spatial -OMICS technologies facilitate the interrogation of molecular profiles in the context of the underlying histopathology and tissue microenvironment. Paired analysis of histopathology and molecular data can provide pathologists with otherwise unobtainable insights into biological mechanisms. To connect the disparate molecular and histopathologic features into a single workspace, we developed FUSION (Functional Unit State IdentificatiON in WSIs [Whole Slide Images]), a web-based tool that provides users with a broad array of visualization and analytical tools including deep learning-based algorithms for in-depth interrogation of spatial -OMICS datasets and their associated high-resolution histology images. FUSION enables end-to-end analysis of functional tissue units (FTUs), automatically aggregating underlying molecular data to provide a histopathology-based medium for analyzing healthy and altered cell states and driving new discoveries using "pathomic" features. We demonstrate FUSION using 10x Visium spatial transcriptomics (ST) data from both formalin-fixed paraffin embedded (FFPE) and frozen prepared datasets consisting of healthy and diseased tissue. Through several use-cases, we demonstrate how users can identify spatial linkages between quantitative pathomics, qualitative image characteristics, and spatial --omics.

Dampak Hubungan Bilateral Indonesia dengan Jepang Sebelum dan Sesudah Indonesia-Japan Economic Partnership Agreement (IJEPA)

his study aims to determine whether or not there is a difference in the volume of Indonesian exports to Japan, Indonesian imports from Japan, Indonesian foreign investment from Japan and exchange rates before and after IJEPA. This study uses secondary data obtained from the Central Statistics Agency and the Investment Coordinating Board with a period of 9 years before and after IJEPA starting in 2000 - 2017 with a paired sample t-test analysis (paired t test). The results showed that there was no significant difference between Indonesian exports to Japan before and after IJEPA and there were significant differences between Indonesian imports from Japan, Indonesian foreign investment from Japan and the exchange rate before and after IJEPA.

SHORT-TERM OUTCOME OF A MODERN MONOBLOCK TITANIUM FLUTED TAPERED REVISION STEM FOR COMPLEX PRIMARY AND REVISION TOTAL HIP ARTHROPLASTY

For over a decade, modular titanium fluted tapered (TFT) stems have demonstrated excellent clinical success for femoral revision total hip arthroplasty (THA) surgery. The aim of this study was to report the short-term outcomes of a novel modern monoblock TFT stem used for revision and complex primary THA with a minimum of 2 years follow-up.We identified 126 patients who received a single monoblock TFT stem - 26 patients for complex THA (failed fracture fixation) and 100 patients for revision THA. The reasons for revision THA included 40 for previous prosthetic joint infection (PJI), 42 for aseptic loosening, 9 for trunnionosis, 9 for periprosthetic fractures. The Paprosky grading for femoral bone loss at the time of surgery and the measured subsidence of femoral stems at 3 months follow-up were determined. We evaluated the number and indications for re-operations.The mean time from surgery was 3.9 years (range 2.0 to 6.9 years). A paired t-test analysis showed significant improvement from pre-operative versus post-operative clinical outcome scores (p&lt;0.001) for HHS (38.76 +/- 15.24vs. 83.42 +/- 15.38), WOMAC (45.6 ± 19.0 vs. 69.9 ± 21.3) and SF-12 Physical component (31.7 ± 8.1 vs. 37.8 ± 11.3) and SF-12 Mental component (48.2 ± 12.2 vs. 51.6 ± 12.5). The Paprosky grading for femoral bone loss was Grade 1 (3.9%), Grade 2 (35.7%), Grade 3A (47.6%), Grade 3B (11.1%) and Grade 4 (1.6%) cases. There were 18 re-operations (14.7%) with 13 for PJI (7 treated with implant retention, 6 treated with a two-staged revision), 4 for instability and one for acetabular aseptic loosening. There were no aseptic failures of the stem.This novel modern monoblock TFT stem provided reliable femoral fixation and has increasingly supplanted the use of modular TFT stems for complex primary and revision surgery in our institution.

PENGARUH MEDIA BIG BOOK TERHADAP PERKEMBANGAN KOSAKATA BAHASA INGGRIS SISWA KELAS I SD NEGERI 040443 KABANJAHE

The study aims to determine the development of English vocabulary of children aged 6-7 years through Big Book media on students in class I of SD Negeri 040443 Kabanjahe. This study uses an experimental research method because it will look for the effect of certain treatments. The type of research is the Quasi Experiment. The quasi-experimental research design used in this research is the Nonequivalent control group design. In this design there are two groups used, namely the experimental group and the control group. The population used in this research was all class I138students at SD Negeri 040443 Kabanjahe, totaling 40 children, with the sample being classes B1 and B2 determined by purposive sampling. Data collection techniques by conducting observations, questions, and answers (interviews), and taking physical evidence such as photographs (documents), and RPPH. Before being used, the instrument was tested for validity and reliability. Validity testing with the Corrected Item Total Correlation technique and reliability testing with Cronbach Alpha's technique. All calculations use the help of the SPSS program version 18.0 for windows. Furthermore, to meet the requirements of the hypothesis test, normality calculations were carried out with the Kolmogorov-Smirnov test and homogeneity calculations with the Lavene Statistic test on both samples (class B1 and class B2). Data analysis techniques were carried out with descriptive statistics and continued with inferential statistics using the t-test or t-test, with the formula paired sample t-test and a significance value of α = 0.05 (5%). Based on the results of the study, it was concluded that the use of Big Book media in the English learning process on vocabulary proved to have a significant effect on the development of children's English vocabulary in the experimental group in class B1. The level of vocabulary development of children who were given treatment was higher compared to the group of children who were not given treatment, in the experimental group and the control group there was a "quite significant" difference. Based on calculations carried out with the help of the SPSS version 18.0 for Windows program, it is known that the value of t in the SPSS output after the paired samples T-test analysis was -5.315. Where in the t value there is a minus sign (-) which is a sign that there is a difference between the two groups. Therefore, the presence of this minus sign indicates a signal that there is a difference between the two groups (experimental and control).

Research on the Socio-Spatial Resilience Evaluation and Evolution of the Central Area of Beijing in Transitional China

In recent decades, the increasing threats of global climate change, natural disasters, and epidemics have brought extensive attention to resilience theory. However, most studies focus on the physical aspects of cities, overlooking the significance of the social perspective. This study addresses this gap by using social areas as the core spatial unit for analysis. By constructing a scientific indicator system and employing the set pair analysis method, this study comprehensively evaluates the multidimensional resilience levels of Beijing’s central area during its transitional period (1990–2020). The findings reveal that socio-spatial divisions, which emphasize the network structure of social relations and the dynamic changes in social spaces, are more suitable than traditional administrative divisions as fundamental units for resilience assessment. During Beijing’s transitional period, the comprehensive resilience of the central area and all social areas steadily increased, although social capital resilience faced the challenges of loss and slow recovery. Social areas with a unique internal connection, such as those based on ethnicity, exhibited more positive social capital resilience development. Additionally, social areas with lower population diversity and greater homogeneity tended to have higher comprehensive resilience and a more positive trend in social capital resilience development, whereas highly heterogeneous social areas faced significant constraints in resilience development. These findings may provide valuable insights for cities and communities to enhance their capacity to address future uncertainties and assist policymakers in making informed governance decisions.

Differential EBV protein-specific antibody response between responders and non-responders to EBVSTs immunotherapy.

Epstein-Barr virus (EBV) is associated with a diverse range of lymphomas. EBV-specific T-cell (EBVST) immunotherapies have shown promise in safety and clinical effectiveness in treating EBV-associated lymphomas, but not all patients respond to treatment. To identify the set of EBV-directed antibody responses associated with clinical response in patients with EBV-associated lymphomas, we comprehensively characterized the immune response to the complete EBV proteome using a custom protein microarray in 56 EBV-associated lymphoma patients who were treated with EBVST infusions enrolled in Phase I clinical trials. Significant differences in antibody profiles between responders and non-responders emerged at 3 months post-EBVST infusion. Twenty-five IgG antibodies were present at significantly higher levels in non-responders compared to responders at 3 months post-EBVST infusion, and 10 of these IgG antibody associations remained after adjustment for sex, age, and cancer diagnosis type. Random forest prediction analysis further confirmed that these 10 antibodies were important for predicting clinical response. Differential IgG antibody responses were directed against LMP2A (four fragments), BGRF1/BDRF1 (two fragments), LMP1, BKRF2, BKRF4, and BALF5. Paired analyses using blood samples collected at both pre-infusion and 3 months post-EBVST infusion indicated an increase in the mean antibody level for six other anti-EBV antibodies (IgG: BGLF2, LF1, BGLF3; IgA: BGLF3, BALF2, BBLF2/3) in non-responders. Overall, our results indicate that EBV-directed antibodies can be biomarkers for predicting the clinical response of individuals with EBV-associated lymphomas treated with EBVST infusions.

Innovating Ferro-sonication approach for extracting microplastics from wastewater

For accurate and reliable analysis of microplastics (MPs) in wastewater (WW), it is imperative to comprehend the significance of pre-treating WW before analysis. The suspended solids (SS) in the matrix tend to adhere to the MPs during filtration, which interferes with the detection of the MPs. In this regard, the present study aims to develop and optimize a pretreatment method to improve the extraction efficiency of MPs from WW by reducing the SS. A combination of the Fenton reaction and ultrasonication, ferro-sonication (Fe-UlS), was proposed to digest and eliminate the SS from WW. This hybrid pretreatment, Fe-UlS, was optimized for ultrasonication amplitude, treatment time, and hydrogen peroxide dose using response surface methodology (RSM) with a Box-Behnken design, achieving a desirability of 0.984. The optimum conditions for the Fe-UlS, such as the (1:1) Fenton reagent ratio (0.05 M FeSO4: 30 % H2O2), ultrasonication amplitude (31 %), and total process time (30 min) were found to be statistically significant (p < 0.05). The developed method was then employed for the extraction of spiked polyethylene (PE), polypropylene (PP) and polyethylene terephthalate (PET) MPs in real WW and found efficient in removing 83 % of the TSS present in the primary influent were in 30 min at a temperature of 45 °C. Also, the method did not affect the physio-chemical characteristics of the MPs; however, the thermal analysis of PE and PP MPs showed a statistically significant decrease in the melting temperature, as proven by paired t-test analysis. Further, a non-targeted liquid chromatography-mass spectrometry (LC-MS) analysis proved that Fe-UlS is a stable process, as it did not cause any leaching of MPs under the optimum pretreatment conditions. Finally, Laser Direct-Infrared Imaging (LD-IR) analysis was conducted to validate the developed Fe-UlS pretreatment approach for MP analysis in real WW. About 3434 MPs were detected in 100 mL of WW primary influent, within the size range of 9 to 500 μm. This hybrid pretreatment approach not only streamlines WW sample processing but also reduces the required concentration of Fenton reagent and processing time, yielding accurate and reliable results for monitoring MPs in WW.

High-Dose Drug-Coated Balloon Versus Polymer-Based Drug-Eluting Stent for Femoropopliteal Artery Disease Treatment.

Clinical trials have demonstrated that high-dose drug-coated balloon (HD-DCB) and polymer-based drug-eluting stent (PB-DES) treatments for femoropopliteal (FP) artery disease have favorable outcomes. However, which one would be better remained unrevealed. This study used the databases of 2 large-scale multicenter prospective drug-coated balloon (DCB) and drug-eluting stent (DES) registries. The study included 2470 patients with symptomatic FP lesion treated with IN.PACT Admiral DCB or Eluvia DES at 69 centers. A propensity-score-based paired analysis was conducted. Primary endpoint was 1-year restenosis rate. Secondary endpoints were 1-year reocclusion rate, target lesion revascularization (TLR), acute thrombosis, bypass conversion, major amputation, major adverse limb event (MALE), and all-cause death. A total of 1535 patients were treated with HD-DCB, and 935 patients were treated with PB-DES. The propensity-score matching extracted 678 pairs, with no remarkable intergroup difference in baseline characteristics. The 1-year restenosis rate was significantly lower in the PB-DES group than in the HD-DCB group (16.0% vs 22.0%, p=0.016). The other endpoints (reocclusion rate, TLR, acute thrombosis, bypass conversion, major amputation, MALE, and all-cause death) did not differ between the groups. No baseline characteristics had any significant interaction effect on the association of HD-DCB vs PB-DES with restenosis risk (all p>0.05). This study demonstrated that the 1-year TLR, reocclusion rate, and other endpoints did not differ between the PB-DES group and the HD-DCB group despite the lower restenosis in the PB-DES group. One-year restenosis rate was significantly lower in the polymer-based DES group than in the high-dose DCB group for foemoropopliteal disease. However, there is no difference in the other endpoints between two groups.

Effects of Sacubitril/Valsartan on Survival in Patients with Heart Failure and Significant Valvular Heart Disease.

Although the benefits of sacubitril/valsartan in heart failure with reduced ejection fraction (HFrEF) are well established, patients with hemodynamically significant mitral regurgitation (MR) were excluded from pivotal trials. We aimed to assess the effects of sacubitril/valsartan on survival in patients with HFrEF and concomitant significant MR. All patients from a single center who underwent echocardiography between June 2008 and December 2020, with a left ventricular ejection fraction (LVEF) of less than 40% and hemodynamically significant MR were recruited. Patients were categorized according to drug use and year of the index echocardiogram into the angiotensin receptor/neprilysin inhibitor (ARNI), non-ARNI before 2017, and non-ARNI after 2017 groups. Patients in the ARNI and non-ARNI after 2017 groups were compared directly, whereas patients in the non-ARNI before 2017 group were matched to the ARNI group in a 3:1 ratio. The outcome of interest was all-cause mortality. Death was compared between the groups using univariate and multivariate Cox proportional hazard models. After exclusion by criteria and matching, there remained 610 patients in the ARNI group, 434 in the non-ARNI after 2017 group, and 1,722 in the non-ARNI before 2017 group. During follow-up, all-cause mortality was significantly lower in the ARNI group compared with both non-ARNI after 2017 and non-ARNI before 2017 groups. Multivariate analysis of both pairs of comparison between groups found the use of ARNI to be significantly associated with increased survival. In patients with HFrEF and concomitant significant MR, treatment with sacubitril/valsartan was associated with lower risks of all-cause death.

Identification of regulatory networks and crosstalk factors in brown adipose tissue and liver of a cold-exposed cardiometabolic mouse model

BackgroundActivation of brown adipose tissue (BAT) has gained attention due to its ability to dissipate energy and counteract cardiometabolic diseases (CMDs).MethodsThis study investigated the consequences of cold exposure on the BAT and liver proteomes of an established CMD mouse model based on LDL receptor-deficient (LdlrKO) mice fed a high-fat, high-sucrose, high-cholesterol diet for 16 weeks. We analyzed energy metabolism in vivo and performed untargeted proteomics on BAT and liver of LdlrKO mice maintained at 22 °C or 5 °C for 7 days.ResultsWe identified several dysregulated pathways, miRNAs, and transcription factors in BAT and liver of cold-exposed Ldlrko mice that have not been previously described in this context. Networks of regulatory interactions based on shared downstream targets and analysis of ligand-receptor pairs identified fibrinogen alpha chain (FGA) and fibronectin 1 (FN1) as potential crosstalk factors between BAT and liver in response to cold exposure. Importantly, genetic variations in the genes encoding FGA and FN1 have been associated with cardiometabolic-related phenotypes and traits in humans.DiscussionThis study describes the key factors, pathways, and regulatory networks involved in the crosstalk between BAT and the liver in a cold-exposed CMD mouse model. These findings may provide a basis for future studies aimed at testing whether molecular mediators, as well as regulatory and signaling mechanisms involved in tissue adaption upon cold exposure, could represent a target in cardiometabolic disorders.Graphical abstract