Paddy Field Soil Research Articles

Top-down predators shape soil bacterial community composition while bottom-up nutrients drive bacterial abundance

Although the top-down and bottom-up concept in microbial food-webs has been a primary interest in ecology, less is still known about it in soil ecosystems. Protists are the primary top-down predators of bacterial communities, altering their compositions, while the bottom-up resources are the primary factors limiting bacterial growth. Here, we hypothesized that the top-down predators modulate soil bacterial community composition, while the bottom-up nutrients control the bacterial growth and population. To precisely control nutrient levels, we used an inert soil substitute consisting of a combination of calcined clay and sand. Nutrients equivalent to the reference paddy field soil were added to microcosms as a control treatment. To investigate the effects of C, N, and P, six additional bottom-up treatments in the absence and double amounts of the nutrients were prepared. Four top-down treatments (no protist addition, Acanthamoeba castellanii, Vermamoeba vermiformis, and Heteromita globosa) were set up for each bottom-up treatment. A total of 252 microcosms under 28 treatments were incubated. Bacterial communities were analyzed using high-throughput sequencing and real-time PCR in the 1st, 3rd, and 5th weeks. The results revealed that the top-down predators significantly altered the bacterial community composition, and the bacterial population was predominantly controlled by the bottom-up nutrients. Analysis of absolute abundance data demonstrated that both top-down and bottom-up factors shaped the bacterial community structure (community composition and population). Random forest analysis classified the amplicon sequence variants associated with the treatments, showing that mostly similar families were affected by both bottom-up and top-down factors. In conclusion, the results of this study fully supported our hypothesis that top-down predators alter community composition, while bottom-up factors influence bacterial population dynamics.

Spatial and temporal correlation between soil and rice relative yield in small-scale paddy fields and management zones

Spatial and temporal correlation between soil and rice relative yield in small-scale paddy fields and management zones

Evaluation of climate conditions and ecological traits that limit the distribution expansion of alien Lolium rigidum in Japan

Invasive alien plants cause severe global problems; therefore, determining the factors that lead to the success or failure of invasion is a critical question in the field of invasion ecology. In this study, we aimed to determine the factors underlying differences in the distribution range of alien plants in Japan by investigating why Lolium multiflorum thrives in a wide range of habitats while L. rigidum is mainly distributed on sandy beaches. We initially evaluated environmental niche suitability through species distribution modelling and subsequently examined whether species traits influence the differences in range expansion between the two species. We used MaxEnt modelling to identify potential environmental niches for both species. The analysis revealed that L. rigidum was considerably less suited to the Japanese climate compared to L. multiflorum, with high summer precipitation in Japan identified as one of the climatic factors limiting the distribution of L. rigidum. Given that these winter annual plants remain dormant as seeds during summer, in subsequent experiments, we buried seeds in paddy field soil and sandy beach sand during summer and evaluated their survival rate in autumn. The survival rate of L. rigidum seeds was significantly lower than that of L. multiflorum, particularly in paddy soil. Factors contributing to seed mortality may include the decay or early germination of L. rigidum seeds under Japan’s high rainfall conditions. This study emphasises the importance of considering local environmental factors alongside climate niche modelling in the risk assessment of invasive species. Moreover, the integration of species distribution modelling for large-scale evaluations and manipulation experiments for fine-scale assessments proved effective in identifying climatic conditions and species traits influencing the success or failure of alien species invasion.

Isolation of Heavy Metal-Tolerant and Anti-Phytopathogenic Plant Growth-Promoting Bacteria from Soils.

In this study, by isolating multifunctional soil bacteria that can promote plant development, resist heavy metals, exhibit anti-phytopathogenic action against plant diseases, and produce extracellular enzymes, we hope to improve the effectiveness of phytoremediation techniques. To isolate multifunctional soil bacteria, we used soils with diverse characteristics as isolation sources. To look into the diversity and structural traits of the bacterial communities, We conducted amplicon sequencing of the 16S rRNA gene on five types of soils and predicted functional genes using Tax4Fun2. The isolated bacteria were evaluated for their multifunctional capabilities, including heavy metal tolerance, plant growth promotion, anti-phytopathogenic activity, and extracellular enzyme activity. The genes related to plant growth promotion and anti-phytopathogenic activity were most abundant in forest and paddy soils. Burkholderia sp. FZ3 and FZ5 demonstrated excellent heavy metal resistance (≤ 1 mM Cd and ≤ 10 mM Zn), Pantoea sp. FC24 exhibited the highest protease activity (24.90 μmol tyrosine·g-DCW-1·h-1), and Enterobacter sp. PC20 showed superior plant growth promotion, especially in siderophore production. The multifunctional bacteria isolated using traditional methods included three strains (FC24, FZ3, and FZ5) from the forest and one strain (PC20) from paddy field soil. These results indicate that, for the isolation of beneficial soil microorganisms, utilizing target gene information obtained from isolation sources and subsequently exploring target microorganisms is a valuable strategy.

Health Risk Assessment for Potential Toxic Elements in the Soil and Rice of Typical Paddy Fields in Henan Province.

The accumulation of potential toxic elements in agricultural soil and rice is of particular concern in China. However, studies on the risk assessment of these elements in regional soil-rice systems remain limited. The aim of this study is to evaluate the pollution status and potential health risk of potential toxic elements in typical paddy soil and rice in Henan Province. A total of 80 soil samples and corresponding rice samples were collected to determine the contents of Cd, Pb, As, Cr, Cu, Zn, and Ni, and to assess their potential health risks to local consumers. Results showed that the average contents of these elements in soils were below the national risk screening values in GB15618-2018. Only the average content of Cr in rice exceeded the limit in GB 2762-2022 specified by the national food safety standard. The rates of exceeding the limits for Cd, Pb, As, and Cr in rice samples were 13.89%, 15.28%, 15.28%, and 27.78%, respectively. The health risk assessment indicated that rice intake for both adults and children caused carcinogenic and non-carcinogenic health risks to varying degrees. Local residents are advised to purchase rice from outside the study area to meet their daily needs and strictly regulate the pollution of potential toxic elements within the area.

Enhanced methane and energy generation from sewage water using microbial fuel cells with paddy field soil substrate

Microbial fuel cells (MFCs) have potential in wastewater treatment, biogas production and clean energy generation. MFCs provide an interdisciplinary research approach incorporating engineering and natural sciences. This study explores MFCs’ capabilities to produce electricity and biogas from wastewater and field soil substrates with different compositions. A two-chamber MFC system was operated anaerobically. Household sewage water used as the organic substrate with different soil amounts. Six different process feed compositions, labeled MFC-1–6, were investigated. MFC-1 exhibited the highest biogas generation volume of 245 cm³ and 42 mW/cm² power density. MFC-5 and −6 yielded 100 cm³ and 130 cm³, respectively. Wastewater treatment was effective on day 20, with pH, conductivity, turbidity, and total dissolved solids decreased to 7.3, 2.6 mS, 326 NTUs, and 1114 mg/L, respectively. Since, MFC-1 autonomously generated −800 mV, an external battery supplied an additional 600 mV to meet the methane generation voltage requirements. MFCs’ effectiveness in addressing wastewater treatment and renewable energy production was highlighted.

Design and experiment of bionic skateboard for rice direct seeding machine based on loach body surface

Aiming at the problem of heavy adhesion and large resistance of the rice direct seeding machine slide in the disturbed saturated paddy field environment, the non-smooth surface of the loach body was observed by microscopic observation; the simulation analysis was carried out using Fluent software, revealing the principle of reducing adhesion and drag of the non-smooth surface; the Box-Behnken response surface method was used to design the experiment, and the regression equation of the total resistance of the groove non-smooth surface FBand the groove width w, groove depth hand incoming flow velocity was obtained. vThe optimization analysis results show that when wis 4 mm、v1.25 m/s, 4.5 mm、hthe maximum drag reduction rate reaches 10.052%; based on the optimal parameters, the rice direct seeding machine bionic slide was trial-produced, and the indoor paddy field soil trough test was carried out, and the final drag reduction rate reached 10.23%. This study can provide new ideas for the development of adhesion and drag reduction technology for paddy field soil contact parts.

Isolation, Screening and Biocidal Activity of Secondary Metabolites from Fungi of Paddy Field Soil Samples in Selected Alanganallur Sub Regions, Madurai, Tamil Nadu

Isolation, Screening and Biocidal Activity of Secondary Metabolites from Fungi of Paddy Field Soil Samples in Selected Alanganallur Sub Regions, Madurai, Tamil Nadu

Characteristics of the Soil Organic Carbon Pool in Paddy Fields in Guangdong Province, South China

To understand the role of paddy soils in the global carbon cycle, it is necessary to analyze the characteristics of the organic carbon pool at different soil depths. It was hypothesized that soil organic carbon fractions including labile organic carbon fraction I (LOCF-I), labile organic carbon fraction II (LOCF-II), and recalcitrant organic carbon (ROC) distributed differently within the soil profile. In this study, soil was collected from 27 typical rice fields in Guangdong Province, south China. The carbon fractions of the paddy field soils were analyzed and compared over a 0–60 cm depth profile. The relationship between carbon content and the physical and chemical properties of the soils was further analyzed using correlation analysis and structural equation modeling. The results showed that soil total organic carbon concentration in paddy fields was increased by 22.1% during the last four decades. In the soil organic carbon pool of 0–60 cm profile, the proportion of 67.31 to 70.31% in ROC, 21.75 to 22.06% in LOCF-I, and 7.7 to 10.63% was recorded, respectively, indicating that ROC was the dominating fraction. Storage of soil total organic carbon and fractions all decreased with the increase in soil depth. Correlation and path analysis showed that total nitrogen was the main driving factor affecting the soil carbon fractions, whereas pH and soil bulk density indirectly affected the content of carbon fractions by influencing total nitrogen. The results imply the importance of soil total nitrogen in paddy carbon management of rice cultivation.

Macro-Nutrient Prediction of Paddy Field Soil Using Artificial Neural Network and NIR Spectroscopy

Understanding soil fertility, influenced by macronutrients like nitrogen, phosphorus, and potassium, is essential for adaptive agriculture implementation based on various soil conditions. Near-infrared spectroscopy technology provides non-destructive, rapid soil property measurements without chemicals, applicable both in-field and in-laboratory. However, the wide NIR spectrum range and neural network complexities can hinder Artificial Neural Network (ANN) training and inference, leading to time and resource inefficiency, especially without sophisticated computing devices. This study examines data reduction methods to enhance ANN performance in predicting soil macronutrients using NIR spectra. Multiple Linear Regression (MLR) and Principal Component Analysis (PCA) were applied to select wavelengths from the 1000–2500 nm for ANN input, comparing their performance. About 237 NIR reflectance data from paddy soil were transformed into absorbance data. MLR used forward selection to identify wavelengths with correlations higher than 0.9, while PCA selected wavelengths corresponding to the loading factor peaks for each principal component. These selected wavelengths served as inputs for the ANN model. The ANN’s performance was assessed using correlation and determination coefficients, RMSE, RPD, and model consistency. For nitrogen, the PCA+ANN model with reflectance spectra performed better (RPD 2.4-4.8) than the MLR+ANN model (RPD 2.2-2.6) using fewer wavelengths (5-9 for PCA+ANN vs. 9-12 for MLR+ANN). For phosphorus estimation, the PCA+ANN model also excelled (RPD 2.3-7.0 vs. 2.3-2.4) with fewer wavelengths (4-7 vs. 7). For potassium estimation, the PCA+ANN model showed superior performance (RPD 4.3-9.5 vs. 4.2-4.4), using the same number of wavelengths (4-8 vs. 4-6).

Phytoavailability, translocation, and accompanying isotopic fractionation of cadmium in soil and rice plants in paddy fields

Rice consumption is a major pathway for human cadmium (Cd) exposure. Understanding Cd behavior in the soil-rice system, especially under field conditions, is pivotal for controlling Cd accumulation. This study analyzed Cd concentrations and isotope compositions (δ114/110Cd) in rice plants and surface soil sampled at different times, along with urinary Cd of residents from typical Cd-contaminated paddy fields in Youxian, Hunan, China. Soil water-soluble Cd concentrations varied across sampling times, with δ114/110Cdwater lighter under drained than flooded conditions, suggesting supplementation of water-soluble Cd by isotopically lighter Cd pools, increasing Cd phytoavailability. Both water-soluble Cd and atmospheric deposition contributed to rice Cd accumulation. Water-soluble Cd’s contribution increased from 28‐52% under flooded to 58‐87% under drained conditions due to increased soil Cd phytoavailability. Atmospheric deposition’s contribution (12‐72%) increased with potential atmospheric deposition flux among sampling areas. The enrichment of heavy Cd isotopes occurred from root-stem-grain to prevent rice Cd accumulation. The different extent of enrichment of heavy isotopes in urine indicated different Cd exposure sources. These findings provide valuable insights into the speciation and phytoavailability changes of Cd in the soil-rice system and highlight the potential application of Cd isotopic fingerprinting in understanding the environmental fate of Cd.

Special Issue: Recent advances in biology and fertility studies of paddy field soil

Special Issue: Recent advances in biology and fertility studies of paddy field soil

Quantifying the impact of factors on soil available arsenic using machine learning

Arsenic (As) can accumulate in edible plant parts and thus pose a serious threat to human health. Identifying the contributions of various factors to soil available As is crucial for evaluating environmental risks. However, research quantitatively assessing the importance of soil properties on available As is scarce. In this study, we utilized 442 datasets covering total As, available As, and properties of farmland soils. The five machine learning models were employed to predict soil available As content, and the model with the best predictive performance was selected to calculate the importance of soil properties on available As and interpret the model results. The Random Forest model exhibited the best predictive performance, with R2 for the test set of dryland and paddy fields being 0.83 and 0.82 respectively, while also outperforming other machine learning models in terms of accuracy. Concurrently, evaluating the contribution of soil properties to soil available As revealed that increases in soil total arsenic, pH, organic matter (OM), and cation exchange capacity (CEC) led to higher soil available As content. Among these factors, soil total As had the greatest impact, followed by CEC. The influence of pH on soil available As was greater in dryland compared to OM, while in paddy fields, it was smaller than OM (p < 0.01). Sensitivity analysis results indicated that reducing soil total As content had the greatest effect on available As. In both dryland and paddy field soils, reducing soil total As had the most pronounced effect on available As, leading to reductions of 10.09% and 8.48%, respectively. Therefore, prioritizing the regulation of soil total As and CEC is crucial in As contamination management practices to alter As availability in farmland soils.

Editorial: Recent advances in biology and fertility studies of paddy field soil

Editorial: Recent advances in biology and fertility studies of paddy field soil

How does the biochar-supported sulfidized nanoscale zero-valent iron affect the soil environment and microorganisms while remediating cadmium contaminated paddy soil?

In previous studies, iron-based nanomaterials, especially biochar (BC)-supported sulfidized nanoscale zero-valent iron (S-nZVI/BC), have been widely used for the remediation of soil contaminants. However, its potential risks to the soil ecological environment are still unknown. This study aims to explore the effects of 3% added S-nZVI/BC on soil environment and microorganisms during the remediation of Cd contaminated yellow-brown soil of paddy field. The results showed that after 49 d of incubation, S-nZVI/BC significantly reduced physiologically based extraction test (PBET) extractable Cd concentration (P < 0.05), and increased the immobilization efficiency of Cd by 16.51% and 17.43% compared with S-nZVI and nZVI/BC alone, respectively. Meanwhile, the application of S-nZVI/BC significantly increased soil urease and sucrase activities by 0.153 and 0.446 times, respectively (P < 0.05), improving the soil environmental quality and promoting the soil nitrogen cycle and carbon cycle. The results from the analysis of the 16S rRNA genes indicated that S-nZVI/BC treatment had a minimal effect on the bacterial community and did not appreciably alter the species of the original dominant bacterial phylum. Importantly, compared to other iron-based nanomaterials, incorporating S-nZVI/BC significantly increased the soil organic carbon (OC) content and decreased the excessive release of iron (P < 0.05). This study also found a significant negative correlation between OC content and Fe(II) content (P < 0.05). It might originate from the reducing effect of Fe-reducing bacteria, which consumed OC to promote the reduction of Fe(III). Accompanying this process, the redistribution of Cd and Fe mineral phases in the soil as well as the generation of secondary Fe(II) minerals facilitated Cd immobilization. Overall, S-nZVI/BC could effectively reduce the bioavailability of Cd, increase soil nutrients and enzyme activities, with less toxic impacts on the soil microorganisms.

Investigation of the Microbial Diversity in the Oryza sativa Cultivation Environment and Artificial Transplantation of Microorganisms to Improve Sustainable Mycobiota.

Rice straw is not easy to decompose, it takes a long time to compost, and the anaerobic bacteria involved in the decomposition process produce a large amount of carbon dioxide (CO2), indicating that applications for rice straw need to be developed. Recycling rice straw in agricultural crops is an opportunity to increase the sustainability of grain production. Several studies have shown that the probiotic population gradually decreases in the soil, leading to an increased risk of plant diseases and decreased biomass yield. Because the microorganisms in the soil are related to the growth of plants, when the soil microbial community is imbalanced it seriously affects plant growth. We investigated the feasibility of using composted rice stalks to artificially cultivate microorganisms obtained from the Oryza sativa-planted environment for analyzing the mycobiota and evaluating applications for sustainable agriculture. Microbes obtained from the water-submerged part (group-A) and soil part (group-B) of O. sativa were cultured in an artificial medium, and the microbial diversity was analyzed with internal transcribed spacer sequencing. Paddy field soil was mixed with fermented paddy straw compost, and the microbes obtained from the soil used for O. sativa planting were designated as group-C. The paddy fields transplanted with artificially cultured microbes from group-A were designated as group-D and those from group-B were designated as group-E. We found that fungi and yeasts can be cultured in groups-A and -B. These microbes altered the soil mycobiota in the paddy fields after transplantation in groups-D and -E compared to groups-A and -B. Development in O. sativa post treatment with microbial transplantation was observed in the groups-D and -E compared to group-C. These results showed that artificially cultured microorganisms could be efficiently transplanted into the soil and improve the mycobiota. Phytohormones were involved in improving O. sativa growth and rice yield via the submerged part-derived microbial medium (group-D) or the soil part-derived microbial medium (group-E) treatments. Collectively, these fungi and yeasts may be applied in microbial transplantation via rice straw fermentation to repair soil mycobiota imbalances, facilitating plant growth and sustainable agriculture. These fungi and yeasts may be applied in microbial transplantation to repair soil mycobiota imbalances and sustainable agriculture.

Effects of fertilization applications on soil aggregate organic carbon content and assessment of their influencing factors: A meta-analysis

Fertilizer application plays an important role in agricultural soil organic carbon (SOC) and its sustainable management. However, there is a lack of consensus about the mechanisms regarding fertilizer application in the soil on the procedure of carbon sequestration and sink enhancement in the aggregates. In this study, the extent by which various fertilization treatments, fertilization durations, climate conditions, soil texture, and land-use types affected the organic carbon (OC) content of different particle-size aggregates was quantitatively assessed using a meta-analysis of 696 treatment comparisons from 36 published studies. Based on the meta-analysis results, fertilization enhanced the OC contents of macroaggregates and clay and silt particles, particularly through replenishing organic material. Long-term fertilization increased the OC content of macroaggregates (>0.25 mm), while the OC content of the clay and silt particles (<0.053 mm) reached a maximum at 15.4 years of fertilization, after which it reached carbon saturation. Organic fertilizer effectively increased the OC content of small macroaggregates (0.25–2 mm) and microaggregates (0.053–0.25 mm) at lower temperatures and precipitation. Compared with inorganic fertilization, combined organic–inorganic fertilization increased OC contents of all sizes of aggregates in sandy or clay loam soils, but decreased OC contents of microaggregates and clay and silt particles in silt loam soils. All fertilization treatments increased the OC content of all particle-size aggregates in upland soils. In contrast, inorganic fertilization and mixed organic–inorganic fertilization reduced the OC content of microaggregates (0.053–0.25 mm) and clay and silt particles (<0.053 mm) in paddy field soils. In conclusion, the application of organic fertilizers is more conducive to the accumulation of OC in agricultural soil aggregates. Whereas the limiting factors for the accumulation of OC content in soil aggregates vary under different conditions, organic fertilizer inputs should be given more consideration, especially in dryland and coarse-textured soils.

Cadmium phytoremediation potential of Houttuynia cordata: Insights from growth, uptake, and rhizosphere mechanisms

Cadmium (Cd) pollutes 7.0 % of China’s land area. This study examined the potential of Houttuynia cordata for Cd phytoremediation because of its ability to accumulate Cd in its growth matrix. H. cordata were planted in plastic pots filled with paddy field soils having low (LCd), medium (MCd), and high (HCd) Cd levels of 0.19, 0.69, and 2.91 mg/kg, respectively. After six months of growth, harvested plant parts were evaluated for Cd uptake and tolerance mechanisms. Metabolomics and metagenomics approaches were employed to investigate the soil rhizosphere mechanism. Results showed that the average plant biomass increased as soil Cd increased. The biomass Cd contents surpassed the allowable Cd limits for food (≤ 0.2 mg/kg) and medicinal uses (≤ 0.3 mg/kg). Cd contents were higher in H. cordata roots (30.59–86.27 mg/kg) than in other plant parts (0.63–2.90 mg/kg), with significantly increasing values as Cd soil level increased. Phenolic acids, lipids, amino acids and derivatives, organic acids, and alkaloids comprised the majority (69 in MCd vs HCd and 73 % in LCd vs HCd) of the shared upregulated metabolites. In addition, 13 metabolites specific to H. cordata root exudates were significantly increased. The top two principal metabolic pathways were arginine and proline metabolism, and beta-alanine metabolism. H. cordata increased the abundance of Firmicutes and Glomeromycota across all three Cd levels, and also stimulated the growth of Patescibacteria, Rozellomycota, and Claroideoglomus in HCd. Accordingly, H. cordata demonstrated potential for remediation of Cd-contaminated soils, and safety measures for its production and food use must be highly considered.

Shallow wet irrigation reduces nitrogen leaching loss rate in paddy fields by microbial regulation and lowers rate of downward migration of leaching water: a 15N-tracer study.

China consumes 35% of the world's fertilizer every year; however, most of the nitrogen fertilizers, which are essential for rice cultivation, are not used effectively. In this study, factors affecting the nitrogen leaching loss rate were studied in typical soil and rice varieties in South China. The effects of various irrigation measures on rice growth and nitrogen leaching loss were investigated by conducting experiments with eight groups. These groups included traditional irrigation (TI) and shallow wet irrigation (SWI). The TI is a common irrigation method for farmers in South China, maintaining a water layer of 5-8cm depth. For SWI, after establishing a shallow water layer usually maintaining at 1-2cm, paddy is irrigated when the field water level falls to a certain depth, then this process is then repeat as necessary. The nitrogen distribution characteristics were determined using 15N isotope tracing. In addition, the effects of nitrification, denitrification, and microbial composition on soil nitrogen transformation at different depths were studied by microbial functional gene quantification and high-throughput sequencing. The results revealed that in the SWI groups, the total nitrogen leaching loss rate reduced by 0.3-0.8% and the nitrogen use efficiency (NUE) increased by 2.18-4.43% compared with those in the TI groups. After the 15N-labeled nitrogen fertilizer was applied, the main pathways of nitrogen were found to be related to plant absorption and nitrogen residues. Furthermore, paddy soil ammonia-oxidizing archaea were more effective than ammonia-oxidizing bacteria for soil ammonia oxidation by SWI groups. The SWI measures increased the relative abundance of Firmicutes in paddy soil, enhancing the ability of rice to fix nitrogen to produce ammonium nitrogen, thus reducing the dependence of rice on chemical fertilizers. Moreover, SWI enhanced the relative abundance of nirS and nosZ genes within surface soil bacteria, thereby promoting denitrification in the surface soil of paddy fields. SWI also promoted ammonia oxidation and denitrification by increasing the abundance and activity of Proteobacteria, Nitrospirae, and Bacteroidetes. Collectively, SWI effectively reduced the nitrogen leaching loss rate and increase NUE.

Provincial-scale Soil As Migration and Transformation and Rice Safe Planting Zoning: A Case Study of Guizhou Province

To explore the distribution characteristics of paddy soil and rice AS content, as well as the health risks of rice consumption, and to evaluate the safe planting ability of rice, 209 paddy soil samples and 1 567 groups of paddy soil-rice samples were collected, their As content and basic soil physical and chemical properties were determined, and the single-factor pollution index method was used to evaluate the pollution degree of the samples. The results showed that:① the soil of paddy fields in Guizhou Province was mainly neutral, and its fertilizer retention capacity and organic matter content were above the medium level, and the soil was relatively fertile. The range of ω(As) in paddy soil was 0.042-91.75 mg·kg-1, the geometric mean was 10.03 mg·kg-1, and the cumulative effect of paddy soil As was lower than that of natural soil As (P<0.05) by independent sample T. Compared with the screening value (0.2 mg·kg-1) of the Soil Pollution Risk Management and Control Standard for Agricultural Land (GB 15618-2018), the excess rate of soil samples was 15.37%. ② The ω (As) range of rice grain samples was 0.001-0.937 mg·kg-1, the geometric average value was 0.108 mg·kg-1, 10.21% of the rice grain samples exceeded the limit value of "Limit of Contaminants in Food (trial)" (GB 2762-2022), and the locations where the exceedances are mainly found are in the central and northern parts of Qiannan Prefecture, as well as around industrial and mining activity zones in the southern counties and districts of Zunyi. ③ As ingested through rice posed non-carcinogenic risk and carcinogenic risk for adults and children, and the impact on children was greater than that of adults. There is no strict control area for safe rice planting in Guizhou Province, and rice can be safely planted.