Outline-based Morphometrics Research Articles

Evaluation of the wing cell contour to distinguish between Stomoxys bengalensis and Stomoxys sitiens (Diptera: Muscidae) using outline-based morphometrics

The blood-sucking flies of the genus Stomoxys Geoffroy, 1762 (Diptera: Muscidae) are significant ectoparasites that can cause irritation and transmit pathogens to both animals and humans. Within the genus Stomoxys, two species, Stomoxys bengalensis and Stomoxys sitiens, have similar morphology and coexist in the same habitat. Accurate species identification of these flies is crucial for understanding disease vectors and implementing effective control measures. In this study, we assessed the effectiveness of outline-based geometric morphometrics (GM) by analyzing the wing cell contour of the first posterior cell (R5) to distinguish between species and sexes of S. bengalensis and S. sitiens. Our results demonstrate that the outline-based GM method is highly effective in distinguishing between species and sexes of these flies based on contour shape, with accuracy scores ranging from 90.0% to 97.5%. Therefore, outline-based GM emerges as a promising alternative to landmark-based GM or as a supplementary tool in conjunction with traditional morphology-based methods for species identification.

Classification of planetary craters using outline-based morphometrics

The morphologies of craters on planetary surfaces reveal clues about the geologic mechanisms by which they originate and subsequently evolve, as well as the materials and physical variables inherent to the environment in which they formed. We carried out a quantitative multivariate analysis of shape descriptors derived from the outlines of craters formed by volcanic processes on Mars, Io, and Earth and by impact cratering on the Moon using elliptic Fourier analysis (EFA) and the Zahn-Roskies (Z-R) shape function. Canonical variate analysis (CVA) was used to construct a statistical model of differences between the crater groups to classify craters produced by various volcanic and impact processes.The classification model from canonical variate analysis of EFA shape descriptors yielded a 90% rate of success for the assignment of group membership among 406 examined craters. It correctly classified 138 of 154 (90%) ionian paterae,154 of 155 (99%) lunar impact craters, 31 of 35 (89%) terrestrial basaltic shield calderas, 32 of 38 (84%) terrestrial ash-flow calderas, and 12 of 24 (50%) martian basaltic shield calderas. The classification model from canonical variate analysis of Z-R shape function descriptors classified 84% of the total population of the examined craters correctly. The analysis correctly classified 96% of ionian paterae, 100% lunar impact craters, 51% terrestrial basaltic shield calderas, and 63% martian calderas, but only 16% of the terrestrial ash-flow calderas were correctly classified.Canonical variate analysis of EFA and Z-R results shows that the shapes of ash-flow calderas and paterae on Io differ the least of all groups included in this study, and basaltic shield calderas and martian calderas analyzed together also have few differences. The Z-R model successfully classifies more ionian patera and impact craters than the EFA classification model but performs poorly at classifying the other crater groups. This result shows that the descriptors convey different shape information. The Z-R model is robust in its ability to classify end-member differences in complexity while the EFA model is robust in its ability to reliably classify among more groups.These differences and similarities in shape confirm previously understood commonalities related to the origin and evolution of various types of craters. In general, basalt shield calderas on Earth and Mars are morphologically similar and are thought to have similar origins; this study confirms that the 2-D shapes of their craters are quantitatively correlated. Similarities have been noted between terrestrial ash-flow calderas and paterae on Io, principally in their large sizes, shallow magma chambers and complex evolution; this study confirms their shapes are also similar. Impact craters and ionian paterae are most dissimilar, as are their evolutions. This study demonstrates rigorous landform shape analysis can greatly increase our understanding of the diversity in craters and the processes involved in their formation.

Landmark and outline-based geometric morphometrics analysis of three Stomoxys flies (Diptera: Muscidae).

Adult flies of the genus Stomoxys Geoffroy, 1762 (Diptera: Muscidae), especially S. pullus Austen, 1909, S. uruma Shinonaga et Kano, 1966 and S. indicus Picard, 1908, are morphologically similar and sometimes difficult to distinguish when using external morphological characteristics. These species may act as vectors and/or potential vectors of many pathogens (virus, bacteria and protozoa). Their correct identification is important to target the vectors involved in the transmission of the pathogens and also helps in the fly control program.The aim of the present study was to distinguish three species which are difficult to separate using traditional diagnostic characters for species of Stomoxys such as colour patterns and body proportions. Modern morphometrics, both landmark and outline-based, was used to access wing geometry of S. pullus, S. uruma and S. indicus. A total of 198 and 190 wing pictures were analysed for landmark- and outline-based approaches, respectively. Wing shape was able to separate species and sexes of the three Stomoxys flies with highly significant difference of Mahalanobis distances. The cross-validated classification scores ranged from 76% to 100% for landmark and 77% to 96% for outline-based morphometrics. The geometry of wing features appears to be a very useful, low-cost tool to distinguish among the vectors S. pullus, S. uruma and S. indicus.

Morphometry of Equine Distal Sesamoid Bone on Clinically Normal Horses

Shape is a fundamental morphological descriptor, one method of its estimation being from digitally processed images. Elliptic Fourier method is an outline-based morphometrics that has some advantages: it does not require either landmarks or previous knowledge about the shape variation of the objects under study; it can visualize the contour of information and reconstruct the original shape; it can be mathematically normalized to size, rotation and starting point of the contour trace; and it can be conducted automatically using computer software. Hence, the variation could be decomposed into several mutually independent quantitative characteristics. In this manner, unacceptable errors based only on human visual judgment of shape, which is frequently deceptive and misled by size factors, can be effectively eliminated. The present study evaluated the shape of equine distal sesamoids (“navicular bone”) in 28 feet belonging to “Cavall Pirinenc Catala” by means of Elliptic Fourier methods. No allometric relationship (low coefficient of regression) was demonstrated between area and form of the bone. At the same time, different morphological patterns appeared. Shape differences were centred on the proximal ridge, which moved from a crescentic, oval lucency, slightly oval, to a flat contour, and no concave ridge was detected. So breed does not seem to be very prone to suffer from navicular diseases, as some authors have stated that there is an apparent predisposition according to shape of the proximal articular border. These results provide a baseline to further examine shape quantification in sesamoid morphology.

Outline-based morphometrics, an overlooked method in arthropod studies?

Modern methods allow a geometric representation of forms, separating size and shape. In entomology, as well as in many other fields involving arthropod studies, shape variation has proved useful for species identification and population characterization. In medical entomology, it has been applied to very specific questions such as population structure, reinfestation of insecticide-treated areas and cryptic species recognition. For shape comparisons, great importance is given to the quality of landmarks in terms of comparability. Two conceptually and statistically separate approaches are: (i) landmark-based morphometrics, based on the relative position of a few anatomical “true” or “traditional” landmarks, and (ii) outline-based morphometrics, which captures the contour of forms through a sequence of close “pseudo-landmarks”.Most of the studies on insects of medical, veterinary or economic importance make use of the landmark approach. The present survey makes a case for the outline method, here based on elliptic Fourier analysis. The collection of pseudo-landmarks may require the manual digitization of many points and, for this reason, might appear less attractive. It, however, has the ability to compare homologous organs or structures having no landmarks at all. This strength offers the possibility to study a wider range of anatomical structures and thus, a larger range of arthropods.We present a few examples highlighting its interest for separating close or cryptic species, or characterizing conspecific geographic populations, in a series of different vector organisms. In this simple application, i.e. the recognition of close or cryptic forms, the outline approach provided similar scores as those obtained by the landmark-based approach.

Morphometry of Middle Bronze Age palstaves. Part II – spatial distribution of shapes in two typological groups, implications for production and exportation

For archaeologists, metallic artifacts are key materials to assess Middle Bronze Age production areas and cultural exchanges. Here, a set of 629 bronze palstaves excavated in northern France, belonging to Breton and Norman typological groups, was treated by (open) outline-based morphometrics with orthogonal polynomial regression. Using robust statistics developed for outlier detection, these Norman and Breton palstave outlines can be divided into two groups: those for which the shape fluctuates close to the standard shape, called “congruent” axes, and those which are far enough from this standard to be considered as “non-congruent”, although they possess most of the features of the typological group. The highest density of discovery (whether congruent and non-congruent in shape) is in the extreme east of Brittany for the Breton axes, while the Norman axes are concentrated in northern Normandy, hence the choice of names. However, the distribution of congruent and non-congruent artifacts appears to be spatially dependent for the Norman group, and to a lesser extent for the Breton group, as there are proportionally more congruent specimens inside the supposed production areas than outside. This contradicts the generally accepted archaeological scheme which hypothesizes that all axes in a group originate from the same production center, and that some items were exported from there to supply neighboring regions. Other minor production centers probably existed, copying the original model with greater shape variation.