Osteological Data Research Articles

Ancestors of the Oriental Horse in Eurasia: Origin and Distribution

This study discusses the origin and dispersal of the Oriental agile horse, using a range of data—historical, faunal, genetic, and iconographic. It focuses on the Akhal-Teke horses as the model breed of the Oriental horse. Their unambiguous ancestors were horses ridden by the Pazyryk chieftains (400-200 BC). Findings about the Oriental horses, based on the analysis of the Akhal-Teke and Pazyryk breeds, are compared with osteological and iconographic data relating to horses from adjacent territories. This paper looks at horse breeding in Iran and at the Nisaean breed— the earliest one mentioned in written sources. Using the criteria outlined by the prominent Russian horse expert W.O. Witt, the exterior of the Oriental horse is described, and its homeland and dispersal across the neighboring areas are reconstructed. The likely homeland was Central Asia from the Caspian coast to Fergana, and the time of origin is between the beginning of horse riding and military campaigns. The Oriental horse was possibly an outcome of a cross between the domesticated horse from the Middle Volga and the tarpan of the Eurasian or Asian steppes.

The role of birds at Çatalhöyük revealed by the analysis of eggshell

The exceptional eggshell assemblage from Çatalhöyük was studied using an integrated approach combining morphology (by optical and scanning electron microscopy) and palaeoproteomics (by mass spectrometry). We provide taxonomic classification for 90 fragments, of which only 11 remain undetermined. The striking predominance of Anseriformes (probably including greylag geese, as well as ducks and swans) in all types of deposits examined, particularly middens and burial fills, suggests that these eggs were exploited as food and, at the same time, had a special significance for the inhabitants of the site. We detected the presence of crane eggshell in contexts associated with both the world of the living and the world of the dead (consistent with the well-known importance of this bird at Çatalhöyük), as well as the possible but infrequent occurrence of bustards and herons. Overall, we suggest that eggshell analysis, together with osteological data and the archaeological context, can provide the basis for a nuanced understanding of the relationship between humans and birds in the past.

Creating a body-subject in the Late Moche Period (CE 650 – 850). Bioarchaeological and biogeochemical analyses of human offerings from Huaca Colorada, Jequetepeque Valley, Peru

ABSTRACT Human offerings in the archaeological record are commonly defined by their community affiliation, the ceremonial events following their death and the places where they are interred. The deposition of an individual links kin members to the landscape but also seems to mark time and memory. Here we argue that inter-generational memory, created through cyclical depositions of local, coastal community members at Huaca Colorada, reflects political alliances during the Late Moche Period of northern Peru. Using multiple lines of evidence, which include osteological, isotopic and burial context data, this article interprets the human offerings among the Moche of the Andes and argues that the significance of foundation offerings lies not exclusively in the spectacle of sacrifice, but in creating memory that maintains or transforms sacred landscapes.

From the gallows to the grave - investigating the cause of death of seven skeletons from Renaissance Odense.

In 1982, archaeologists recovered seven skeletons at the site of Søndergade, OBM8230 in Odense, Denmark. The skeletons were isolated burials outside of cemetery context. This article examines the osteological and historical data of these seven skeletons and their burial location with the aim of examining the circumstances of their deaths. The chronological dating of the skeletons suggests that the seven individuals were buried around or after the Lutheran Reformation (AD 1536) up until the middle of the 17th century. From historical evidence, we can place the skeletons outside the western city gate, which is also where the wheel and gallows were located in Renaissance Odense. Based on the burial location and position in the grave of these skeletons, we hypothesize that the seven individuals were executed criminals. The results of the skeletal analysis show that all were young males. Two of the individuals have osteological evidence of decapitation. The remaining five individuals were most likely hanged based on historical and archaeological evidence, possibly for the crime of theft (Secher 1929). The historical, archeological and osteological analyses of these seven skeletons support the hypothesis that being buried in unconsecrated ground was a punishment in the form of expulsion of the community in death as the law indicates, rather than the absolution of the criminal's sins in life.

Osteobiography as Microhistory: Writing from the Bones Up

Osteobiography has much potential to address how bodies both emerge from, and contribute to, historical process. Necessarily multiscalar, if not always explicitly framed as such, osteobiography can be used to enhance the connection between individual life histories and macroscale historical themes. Conceptualizing osteobiography as a microhistory extends analysis beyond the individual through entanglements with populations, objects, landscapes, and social phenomena. Microhistory, which has been variously defined and employed by historians and archaeologists alike, is here envisioned as an investigation of the intersections between biography and large-scale, long-term phenomena. In this framing, the skeletal body is viewed as a node conjoining overlapping temporalities, materials, and biographies, thereby combining the lived experience of individuals with other emergent scales. This article explores the possibilities afforded by a microhistorical approach to skeletal remains from an early medieval site in Bohemia (modern Czech Republic). The body is recognized as a site not only for the coalescence of scales but also of different forms of evidence, as textual, archaeological, and osteological data inform the analysis. The osteobiographical narratives that emerge emphasize the relational, the historical, and the contingent in our interpretations of human remains.

Inferring Age at Death for Japanese and Thai Skeletal Samples under a Bayesian Framework of Analysis: A Test of Priors and Their Effects on Estimation

A critical step in age-at-death estimation is to identify the age-at-death distribution to which the unknown skeletal remains most likely belong. Age estimation based on a frequentist approach assumes that the age distribution of the target population is same as that of the reference sample. In a Bayesian framework, researchers have greater flexibility, with the freedom to specify mortality information via a prior distribution and integrate it with osteological data to produce more accurate age estimates. The selection of an optimal prior remains challenging, as forensic anthropologists analyze unidentified individuals often originating from unknown populations. Understanding the effects of the prior choice on the final age produced for various populations is essential to the interpretation of these estimates.In this article we investigate the effects of three different priors on age estimates using scores of three traditional methods collected on Asian skeletal samples representing peoples from Japan and Thailand. We test a uniform prior, which assumes the equal chance of death regardless of age, as well as various informative priors, derived from a Japanese mortality database and skeletal collections. We combine each of the priors with the parameters of the cumulative probit regression model to obtain age estimates. The results of our analyses show that the informative prior outperforms when it is carefully chosen to reflect the geographic and temporal origins of the target population. While the uniform prior produces the least-biasedage estimates, one should be cautious, as it can generate unrealistically old and inaccurate ages.

Darevskia parvula (Lantz & Cyren, 1913) ve Darevskia adjarica (Darevsky & Eiselt, 1980)'nın karşılaştırmalı iskelet osteolojisi (Squamata: Lacertidae)

A detailed description of cranial and postcranial osteology of Darevskia parvula and D. adjarica is provided osteological data for further comparative studies of other lizards. The descriptions and comparisons are based on 12 specimens, of which 10 adult and 2 juveniles for each species. Darevskia parvula and D. adjarica are highly conservative species and they have similar osteological features. The differences in the current study are the number of teeth for the cranial skeleton and the number of vertebrae for the postcranial. Darevskia parvula has a lower number of teeth than D. adjarica.

8. The Old and The Young in Early Medieval Iceland: The Evidence for Three-generational Families in the Household Cemeteries of Skagafjorður, Northern Iceland

In recent years, a number of early Christian cemeteries have been excavated in the region of Skagafjörður, North Iceland. They belonged to, and were managed by, occupants of individual farms and were in use from around AD 1000-1120. These household cemeteries differ from the sparse pagan burial record in that they are ‘all inclusive’, i.e. they include individuals of both sexes and all ages, and essentially represent all inhabitants of a household. An interesting feature of the cemeteries is the large number of interred infants and relatively large number of ‘older’ individuals. By looking at the osteological data in conjunction with the placement of burials within cemeteries a more detailed picture of the Medieval household emerges, including the perception of the young and the aged and the possible role of three-generation families in the transference of knowledge and life experience. This paper discusses how this unique material can add to the predominantly philological and historical discussions on the nature, composition and social interactions of the Medieval Icelandic household.

Cerogobius petrophilus (Perciformes: Gobiidae), a new gobiid genus and species from the Red Sea.

A new genus and species of cryptobenthic goby, Cerogobius petrophilus is described from the Red Sea based on nine specimens not exceeding 2.5 cm in total length, collected from a stone-rubble habitat at Thuwal, Saudi Arabia, at depths of 8-15 m. It was also observed underwater at the southern tip of Ras Mohammed and Marsa Alam in Egypt. Cerogobius petrophilus sp. nov. is unique among other gobies in its habitat, and in this regard it superficially resembles some species of blennies, occupying tight holes in stones covered with short algae. Molecular phylogenetic data suggest a close relationship between Cerogobius petrophilus sp. nov. and Hetereleotris, but the former differs from the latter morphologically in head shape with specific proportions of orbit and snout, forward-set position of eyes, a moderately large mouth, a long horn-like tentacle at the nostrils in the middle of snout, caudal peduncle deep and short, and in details of cephalic sensory system. A full description of the new genus and species is provided and is accompanied with osteological data that potentially can be informative in further comparisons with Hetereleotris.

Sex assessment from the proximal femur in a Spanish population based on three-dimensional computed tomography metric analysis

Background/Aim. The studies published in recent years have shown that the linear measurements on the three-dimensional computed tomography (3D-CT) clinical images of the hip bone, skull or breastbone can serve as a reliable alternative method for sex estimation. In spite of the fact that the proximal femur exhibited high dimorphism when examining the skeletal material, there is still a lack of morphometric studies dealing with the CT imaging of this anatomical region that would confirm the relevance of the previously obtained results. The aim of this study was to validate the reliability and precision of some proximal femur measurements obtained in vivo from the 3D-CT models and to compare the accuracies of our findings with those formerly reported by other relevant research. Methods. A total of 146 CT scans (73 male and 73 females) were selected to take 6 measurements using the traditional osteometric methods. The 3D reconstruction was done at 1mm and 1.25 mm thick slices with OsiriX (v.4.1). The univariate and multivariate discriminant functions (DFs) were formulated for assessing sex. Results. The vertical diameter of neck and the vertical diameter of head were found to contribute the most when considered independently (90.4%?91.8%). When combining these with the other dimensions, the prediction accuracy increased up to 97.3%. The accuracy of CT measurements is in accordance with those obtained in the traditional morphometric studies on the skeletonized femurs of contemporary populations. The 3D-CT approach showed remarkably higher percentage of predictive ability in comparison with the 2D technique. Conclusion. 3D-CT is a suitable tool for the objective quantification of osteological data. The medical scans and measurements on living individuals offer a valuable source of data from which the highly reliable skeletal standards can be developed for estimating sex, even from the fragmented remains. The method proposed here can be highly useful especially in the identification of mass disaster victims when the direct osteometry is difficult to apply and maceration of the remains is not an option.

Elites and Human Sacrifices at Paquimé: A Bioarchaeological Assessment

Various forms of violence have been implicated in the development and cultural trajectory of Paquimé, the center of the Casas Grandes culture in northwestern Chihuahua, Mexico. However, these inferences have received little systematic bioarchaeological investigation. This study focuses on the most complex burial discovered at Paquimé, a subfloor tomb containing the remains of at least 12 individuals in two distinct burial layers. Here we evaluate the hypothesis that the commingled layer of human remains represents a sacred offering to the elite individuals below through a comparative analysis of demography, health indicators, trauma, post-mortem processing, and taphonomy. Results revealed that there are significant differences between the two burial layers, particularly in terms of post-mortem processing, health status, and age-at-death structure. The commingled remains consisted primarily of unhealthy older sub-adults (and one young adult female) whose skeletal remains exhibit extreme post-mortem processing. The articulated burials, on the other hand, were all adults who received elaborate mortuary treatment, were not processed, and appear to have been well-buffered against disease stress during life. Both the osteological and contextual data from this burial tomb are consistent with sacrificial deposits in Mesoamerica and suggest that the commingled remains were companion sacrifices for the elites below. Thus, this study provides bioarchaeological evidence for ritual violence at Paquimé, which likely reflects the outcome of culturally specific ritual and political actors at the site.

Rising from the ashes: resurrection of the Malagasy chameleons Furcifer monoceras and F. voeltzkowi (Squamata: Chamaeleonidae), based on micro-CT scans and external morphology.

The taxonomy of the Malagasy chameleon Furcifer rhinoceratus (Gray, 1845) is poorly resolved. The aim of this study is to clarify the taxonomic status of Chamaeleon voeltzkowi Boettger, 1893 and Chamaeleon monoceras Boettger, 1913 both only known from single or very few specimens mostly collected more than 100 years ago and currently considered as synonyms of Furcifer rhinoceratus. Using osteological data from micro-X-ray computed tomography (micro-CT) combined with traditional morphological characters and morphometrics we resurrect both taxa from the synonymy of F. rhinoceratus as F. voeltzkowi and F. monoceras, respectively. Compared to F. rhinoceratus, F. monoceras is smaller, has a relatively shorter tail, a longer and thinner rostral appendage, a poorly developed gular crest and no ventral crest, whereas F. voeltzkowi has a smaller rostral appendage, higher casque and the dorsal crest is continuous with the tail crest. Compared to the broad rostral appendage formed by the anterior protuberance of the premaxillary process of the maxilla, which has serrated edges in F. rhinoceratus, F. monoceras presents a long rostral appendage with a smooth dorsal edge that progressively narrows, and the nasal aperture is extended along the elongated appendage; F. voeltzkowi presents a smaller but curved rostral appendage with a crenate edge. The prefrontal and postorbitofrontal approach one another forming a large, laterally closed supraorbital fontanelle in F. rhinoceratus while in F. monoceras they do not approach, leaving a laterally open fontanelle, and in F. voeltzkowi the fontanelle is diminutive. Furcifer voeltzkowi also differs from the similar F. labordi by a smaller size of the rostral appendage, less bulging casque and body pholidosis. The former exhibits a conspicuous white lateral band comprising heterogeneous scalation. Furcifer labordi, on the other hand, has a homogeneous scalation with a remarkable reticulate pattern. Osteologically, the shape of the prefrontal and the connection of the postorbitofrontal with the parietal also differ greatly between the two. Using micro-CT scans we detected key differences that would be otherwise impossible to determine. We also provide a brief morphological and osteological description of the species and strongly recommend efforts to rediscover these two poorly known taxa in order to enable additional studies and to assess their conservation status.

Phylogenetic analysis shows the general diversification pattern of deep-sea notacanthiforms (Teleostei: Elopomorpha)

The Notacanthiformes is an ancient group of deep-sea ray-finned fishes comprising 27 species in two families; Halosauridae and Notacanthidae. Although many studies have tried to reconstruct the phylogenetic relationships among the major clades of Elopomorpha, little is known about the evolutionary history of notacanthiforms. Molecular and morphological data were used to test previous hypotheses regarding the phylogenetic relationships among notacanthiform taxa, and to unravel the origin and evolution of this group. The molecular analyses of notacanthids showed similar results to those previously obtained employing osteological data, which proposed the existence of the Lipogenyinae (Lipogenys) and Notacanthinae (Notacanthus + Polyacanthonotus) subfamilies. Nevertheless, when the external morphology data is considered Lipogenys is more related to Notacanthus than Polyacanthonotus. The analyses could not fully resolve the inner relationships of the halosaurids. The time-calibrated tree of the order Notacanthiformes shows a long process of diversification spanning from the upper Cretaceous, to 50 million years after the K-Pg extinction, with the gradual emergence of all the modern families and genera of the group. This is the first specific phylogeny of the order Notacanthiformes, combining different analyses and data in order to obtain a wider perspective of the evolution and diversification of this group of fishes.

Resurrection and re-description of Plethodontohyla laevis (Boettger, 1913) and transfer of Rhombophryne alluaudi (Mocquard, 1901) to the genus Plethodontohyla (Amphibia, Microhylidae, Cophylinae)

The systematics of the cophyline microhylid frog genera Plethodontohyla and Rhombophryne have long been intertwined, and their relationships have only recently started to become clear. While Rhombophryne has received a lot of recent taxonomic attention, Plethodontohyla has been largely neglected. Our study is a showcase of just how complex the taxonomic situation between these two genera is, and the care that must be taken to resolve taxonomic conundrums where old material, multiple genus transitions, and misattribution of new material obfuscate the picture. We assessed the identity of the historic names Dyscophus alluaudi (currently in the genus Rhombophryne), Phrynocara laeve and Plethodontohyla laevis tsianovohensis (both synonyms of Rhombophryne alluaudi) based on an integrative taxonomic approach harnessing genetics, external morphology, osteological data obtained via micro-Computed Tomography (micro-CT) and bioacoustics. We show that (1) the holotype of Dyscophus alluaudi is a member of the genus Plethodontohyla; (2) the Rhombophryne specimens from central Madagascar currently assigned to Rhombophryne alluaudi have no affinity with that species, and are instead an undescribed species; and (3) Phrynocara laeve and Dyscophus alluaudi are not synonymous, but represent closely related species, whereas Plethodontohyla laevis tsianovohensis is tentatively confirmed as synonym of D. alluaudi. We resurrect and re-describe Plethodontohyla laevis, and re-allocate and re-describe Plethodontohyla alluaudi on the basis of new and historic material.

Sensing the Dead: Mortuary Ritual and Tomb Visitation at Nabataean Petra

The visual prominence of mortuary structures within the Nabataean capital city of Petra clearly indicates that the deceased maintained a constant presence amongst the living. However, current systematic archaeological exploration of these mortuary features documents a more intimate view of the interaction between the living and the dead. Since 1998, the Petra North Ridge Project has sought to record the mortuary practices and gather data on health and diet of non-elite residents of 1st cent. ad Petra. Artifactual, taphonomic, and osteological data indicate that engagement of the city’s inhabitants with the dead occurred through the senses of smell, touch, taste, and sight. Nabataean visits to their familial tombs included ritual feasting and reorganization of the mortuary space, accompanied by the scents of incense, perfumed oils, and quicklime. In addition, mourners left behind material goods that served to illuminate, bedazzle, and entertain the dead. Through this repeated exchange of sensory stimulation, the Nabataeans established and preserved a dynamic relationship with their deceased ancestors.

A systematic reappraisal of the Rufous Potoo Nyctibius bracteatus (Nyctibiidae) and description of a new genus

The Rufous Potoo (Nyctibius bracteatus Gould, 1846) is the smallest member of the family Nyctibiidae, which comprises seven species endemic to the Neotropical region. Morphologically, it is highly distinct from its congeners, not only in its smaller size, but also in possessing a strange marking on its iris and having entirely rufous plumage, which is lightly vermiculated and has pronounced white spots on the wing coverts, breast, belly, flanks and undertail coverts. Molecular studies have shown extraordinarily high levels of genetic divergence amongst potoo species, and a recent genome-scale molecular phylogeny of potoo species placed N. bracteatus as sister to all other potoos with high confidence. Similarly, osteological data reveal that the species has several unique and plesiomorphic characters. Therefore, its distinctive morphology, in combination with unique behavioral traits, and its recurrent placement as the earliest branching terminal in phylogenetic treatments of the family, convinced us to erect a new genus for the species, Phyllaemulor.

A new species of Varanus (Anguimorpha: Varanidae) from the early Miocene of the Czech Republic, and its relationships and palaeoecology

Skeletal remains of a new early Miocene (Ottnangian, MN 4 mammal zone) monitor lizard, Varanus mokrensis sp. nov., are described from two karst fissures in the Mokrá-Western Quarry (1/2001 Turtle Joint; 2/2003 Reptile Joint), Czech Republic, providing the first documented example of a European varanid for which osteological data permit a well-supported assignment to the genus Varanus. The new species is morphologically similar to the Recent Indo-Asiatic varanids of the Varanus bengalensis group. It differs from all other Varanus species on the basis of a single autapomorphy and a combination of 11 characters. As a distinguishing feature of V. mokrensis, the parietal and squamosal processes of the postorbitofrontal form a narrowly acute angle. The teeth show distinct, smooth cutting edges along the mesial and distal margins of the apical portion of their crowns. This feature is not observed in most extant Asiatic Varanus species and may represent a plesiomorphic condition. The results of parsimony phylogenetic analyses, with and without character reweighting, reveal poor resolution within Varanus. A Bayesian analysis shows V. mokrensis to be closely related to extant representatives of the Indo-Asiatic Varanus clade, with close affinities to the V. bengalensis species group. The topology of the Bayesian tree supports the hypothesis that Miocene monitors from Mokrá are representatives of a lineage that is ancestral to the well-defined clade of extant African varanids, including the early Miocene V. rusingensis. In addition, our results support a Eurasian origin for the varanid clade. The extant African Varanus species probably originated in the late Oligocene. The radiation of African varanids probably occurred during the late Oligocene to early Miocene time interval. The occurrence of Varanus in the early Miocene of Mokrá-Western Quarry corresponds to the warm phase of the Miocene Climatic Optimum. Remains of a diverse aquatic and heliophobe amphibian fauna at the 2/2003 Reptile Joint site indicate more humid conditions than those at the 1/2001 Turtle Joint site.http://zoobank.org/urn:lsid:zoobank.org:pub:B1553295-8AC7-42F0-91C4-51C4C13F1C9D

Reassessing museum archaeological collections: unprecedented osteological and ceramic data for the Sucuriju site at the Urubu River, Central Amazon, Brazil

Abstract This article presents laboratory studies of the archaeological collection from the Sucuriju site which has been stored for decades at the technical reserve of the Museu Paraense Emílio Goeldi. These materials result from Mário Simões’ investigations at the Urubu River area, in the municipality of Itacoatiara, Amazonas, Brazil, in the late 1970’s and 80’s. Recently, this region has been hypothesized as a cultural frontier and is considered key to understanding ethnogenetic and cultural interaction processes in late pre-Columbian times. The reassessment of this old collection, which remained untouched for over 20 years, yielded unprecedented osteological (dental) data for the site. The new analysis of Sucuriju ceramics enabled a better understanding of the local ceramic complexes. We highlight the need for integration between past and current research in this region in order to improve archaeological data both at local and regional scales.

Discovery of the oldest Gobius (Teleostei, Gobiiformes) from a marine ecosystem of Early Miocene age

Gobiidae (Gobiiformes, Teleostei) is among the largest families of vertebrates. These fishes are distributed worldwide and contribute significantly to species diversity in marine habitats and reefs. However, their fossil record is sparse prior to the Miocene and little is known about the course of diversification of the clade. Here we report exceptionally well-preserved skeletal remains of the oldest known Gobius from an Early Miocene (Burdigalian) marine ecosystem of Central Europe (Czech Republic). Gobius jarosi Přikryl & Reichenbacher sp. nov. is dated to 19.1–20.4 Ma by biostratigraphical analysis of calcareous nannoplankton from small fragments of the holotype matrix. Gobius jarosi sp. nov. is characterized by a pterygiophore formula of 3-22110 and a premaxilla with a distinctive postmaxillary process, has 11 abdominal and 16–17 caudal vertebrae, six thin spines in the first dorsal fin and one spine and 12 soft rays in the second dorsal fin, one spine and 11 rays in the anal fin, and two anal fin pterygiophores preceding the first haemal spine. Large ctenoid scales cover the body except for its anterior portion and the head. A comparative analysis of meristic and osteological data suggests close affinities between G. jarosi sp. nov. and the extant species G. niger, G. roulei and G. vittatus. Accompanying fish fossils and nannoplankton assemblages indicate that G. jarosi sp. nov., like G. roulei and G. vittatus, lived in an inshore to offshore marine ecosystem. The discovery of such an early member of the lineage leading to the present-day species of Gobius has important implications for the origin and evolution of the Gobiidae, and indicates that diversification of the European Gobiidae began in, but not before, the Early Miocene.http://zoobank.org/urn:lsid:zoobank.org:pub:3229739B-3428-4DFB-9588-4CE875D732D8

Diamond in the rough: a new species of fossorial diamond frog (Rhombophryne) from Ranomafana National Park, southeastern Madagascar

We describe a new species from the cophyline microhylid genus Rhombophryne, a group of fossorial and terrestrial frogs endemic to Madagascar. Found during herpetofaunal surveys of moist montane forest in the remote north of Ranomafana National Park, Rhombophryne nilevina sp. n. exemplifies two difficulties that hinder taxonomic progress in Malagasy cophyline frogs: micro-endemicity and highly secretive habits. Known from only two adult male specimens, this new species is nonetheless easily distinguishable from all other known Rhombophryne using morphological data, and osteological data collected here via X-ray Micro-Computed Tomography, or “micro-CT”. This species is now the largest known Rhombophryne, and the only one known from Ranomafana National Park, which will make it the southern-most member of the genus pending a forthcoming taxonomic revision involving Plethodontohyla and Rhombophryne. Pairwise distances of the mitochondrial 16s rRNA marker show a minimum genetic distance of 4.9% from other nominal Rhombophryne. We also describe recordings of an advertisement call, emitted from a burrow by the holotype. Rhombophryne nilevina sp. n. is not known to be found syntopically with other Rhombophryne, nor to be present elsewhere in Ranomafana National Park, but it probably does co-occur with a few ecologically similar Plethodontohyla species. Although the type locality is within a protected area, we suggest an IUCN listing of Data Deficient for R. nilevina sp. n., as its area of occupancy is largely undetermined within the park.